GTS1 / YGL181W Overview
- Standard Name
- GTS1 1
- Systematic Name
- YGL181W
- SGD ID
- SGD:S000003149
- Aliases
- LSR1 12 , FHT1 13
- Feature Type
- ORF , Verified
- Description
- Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations 1 2 3 4 5 6 7 8 9 10 11
- Name Description
- Glycine Threonine Serine repeat protein 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 396
- Mol. Weight (Da)
- 44407.5
- Isoelectric Point
- 7.53
- Median Abundance (molecules/cell)
- 3323 +/- 1064
- Half-life (hr)
- 6.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all GTS1 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription factor-binding GTPase activator involved in endocytosis and regulation of phosphatidylinositol biosynthesis; localizes to nucleus and actin patches
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription factor binding (IPI)
- enables GTPase activator activity (ISS, IMP)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in endocytosis (IMP)
- involved in negative regulation of phosphatidylinositol biosynthetic process (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in regulation of transcription by RNA polymerase II (IPI)
Cellular Component
- Manually Curated
- located in actin cortical patch (IDA)
- located in cellular bud neck (HDA)
- located in nucleus (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
142 total interactions for 103 unique genes
Physical Interactions
- Affinity Capture-MS: 19
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 7
- Biochemical Activity: 2
- Co-purification: 1
- FRET: 9
- Protein-RNA: 2
- Reconstituted Complex: 2
- Two-hybrid: 29
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Rescue: 4
- Negative Genetic: 38
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 10
- Positive Genetic: 2
- Synthetic Growth Defect: 2
- Synthetic Lethality: 6
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- Gts1p is a transcriptional regulator with an N-terminal Zn-finger and ArfGAP homology domain, a ubiquitin-associated domain, and a C-terminal glutamine-rich region. The cellular level of Gts1p fluctuates periodically, and GTS1 induction affects a variety of phenomena including heat tolerance, endocytosis, glycolytic and respiratory oscillations, production of reactive oxygen species (ROS), and apoptosis. Gts1p appears to affect cell aggregation by binding and inhibiting the transcriptional repressor Sfl1p, which allows the expression of flocculation-related genes. The C-terminal polyglutamine sequence forms Gts1p protein aggregates which trap HSP90 heat-shock proteins, translation elongation factors EF-2, eEF1B, and EF-1 alpha, and mitochondrial proteins Yhb1p and Por1p. Gts1p enhances heat tolerance by activating the HSP104 heat-shock protein and the TPS1 trehalose-phosphate synthase. Gts1p also regulates the production of the HSP70-family proteins Ssa1p and Ssa2p, which inhibit the nuclear localization of Gts1p.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.