DOT6 / YER088C Overview
- Standard Name
- DOT6 1
- Systematic Name
- YER088C
- SGD ID
- SGD:S000000890
- Aliases
- PBF2 4
- Feature Type
- ORF , Verified
- Description
- Protein involved in rRNA and ribosome biogenesis; activated in stochastic pulses of nuclear localization; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; relative distribution to the nucleus increases upon DNA replication stress 1 2 3 4 5 6 7
- Name Description
- Disruptor Of Telomeric silencing 1
- Paralog
- TOD6 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein activated in stochastic pulses of nuclear localization; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; relative distribution to the nucleus increases upon DNA replication stress
- Length (a.a.)
- 670
- Mol. Weight (Da)
- 73064.6
- Isoelectric Point
- 10.15
- Median Abundance (molecules/cell)
- 3048 +/- 769
- Half-life (hr)
- 3.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all DOT6 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding protein involved in chromatin silencing, tRNA gene-mediated silencing, and regulation of RNA polymerase II transcription
View computational annotations
Molecular Function
- Manually Curated
- enables sequence-specific DNA binding (IDA, HDA)
Biological Process
- Manually Curated
- involved in rDNA heterochromatin formation (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in subtelomeric heterochromatin formation (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is resistant to rapamycin; both null mutation and overexpression decrease silencing at rDNA locus and mating type cassette; in systematic studies mutants exhibit sensitivity to tunicamycin and bleomycin, and resistance to hydroxyurea, cycloheximide and fluconazole
Classical Genetics
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- competitive fitness: decreased
- competitive fitness: increased
- haploinsufficient
- innate thermotolerance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- toxin resistance: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The dot6 null mutant is viable; the null mutant of paralog tod6 is viable; the dot6 tod6 double mutant shows decreased repression of rRNA processing genes.
141 total interactions for 121 unique genes
Physical Interactions
- Affinity Capture-MS: 29
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 1
- Biochemical Activity: 2
- Co-localization: 1
- Co-purification: 2
- Two-hybrid: 1
Genetic Interactions
- Dosage Rescue: 9
- Negative Genetic: 76
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 2
- Positive Genetic: 8
- Synthetic Growth Defect: 1
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- DOT6 encodes a helix-turn-helix transcription factor of the Myb family. Dot6p binds PAC (RNA Pol A and C) motifs of the form 5'-GCGATGAGMT-3', which are present in roughly half of all promoters of genes involved in ribosome biogenesis. PAC sites function as Dot6p/Tod6p-dependent repressor elements under stress or poor nutrient conditions. Dot6p functions as a transcriptional repressor whose repressive activity is alleviated by phosphorylation by protein kinase A (PKA) in response to glucose availability. PKA promotes ribosome biogenesis gene expression through inactivation of Dot6p. Glucose deprivation is thereby sufficient to cause repression of ribosome biogenesis. Sch9p responds to glucose availability and phosphorylates Dot6p, possibly reinforcing regulation through PKA. Dot6p appears to effect repression of ribosome biogenesis gene expression at least in part by recruitment of the Rpd3L histone deacetylase complex to ribosome biogenesis gene promoters.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.