DOA1 / YKL213C Overview
- Standard Name
- DOA1 1
- Systematic Name
- YKL213C
- SGD ID
- SGD:S000001696
- Aliases
- UFD3 2 , ZZZ4 12
- Feature Type
- ORF , Verified
- Description
- WD-repeat protein involved in Ub-mediated proteolysis; K48-, K29-specific Ub chain binding protein involved in Ub homeostasis; substrate-recruiting adaptor for Cdc48p in mitochondria-associated degradation; inhibits degradation of Ufd2p-dependent substrates; facilitates proteolysis of Cse4p, a centromeric H3-like protein; required for ribophagy; promotes NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon replication stress 2 3 4 5 6 7 8 9 10 11
- Name Description
- Degradation Of Alpha 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 715
- Mol. Weight (Da)
- 79478.0
- Isoelectric Point
- 4.63
- Median Abundance (molecules/cell)
- 4561 +/- 2166
- Half-life (hr)
- 12.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all DOA1 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ubiquitin-binding protein; involved in ubiquitin homeostasis, proteasome-mediated ubiquitin-dependent protein catabolism, mitochondria-associated ubiquitin-dependent protein catabolism, ribophagy, and NHEJ during stationary phase; localizes to both the nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables K48-linked polyubiquitin modification-dependent protein binding (IDA)
- enables ubiquitin binding (IDA, IMP)
Biological Process
- Manually Curated
- involved in double-strand break repair via nonhomologous end joining (IMP)
- involved in G1 to G0 transition (IMP)
- involved in mitochondria-associated ubiquitin-dependent protein catabolic process (IMP)
- involved in proteasome-mediated ubiquitin-dependent protein catabolic process (IMP, IGI)
- involved in ribophagy (IMP)
- involved in ubiquitin recycling (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows decreased formation of [PSI+] prion, increased sensitivity to DNA-affecting factors (MMS, aminopterin, UV radiation), increased resistance to anesthetic isoflurane and reduced ribophagy under nitrogen starvation; in systematic studies mutation confers sensitivity to numerous chemicals, including cycloheximide, tunicamycin, boric and oleic acids; at the same time mutants are resistant to caffeine, camptothecin, fenpropimorph, fluconazole, mycophenolic acid, quinine
Classical Genetics
- null
- acid pH resistance: decreased
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound excretion: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: increased
- endocytosis: decreased
- heat sensitivity: increased
- ionic stress resistance: decreased
- lipid particle morphology: abnormal
- metal resistance: decreased
- metal resistance: increased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- sporulation efficiency: decreased
- sporulation: absent
- sporulation: decreased
- stress resistance: decreased
- toxin resistance: decreased
- UV resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- viable
- overexpression
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1252 total interactions for 706 unique genes
Physical Interactions
- Affinity Capture-MS: 47
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 22
- Co-crystal Structure: 2
- Co-purification: 5
- Protein-peptide: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 16
- Two-hybrid: 12
Genetic Interactions
- Dosage Growth Defect: 3
- Dosage Lethality: 1
- Dosage Rescue: 9
- Negative Genetic: 853
- Phenotypic Enhancement: 13
- Phenotypic Suppression: 17
- Positive Genetic: 162
- Synthetic Growth Defect: 61
- Synthetic Lethality: 13
- Synthetic Rescue: 11
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.