CCT2 / YIL142W Overview


Standard Name
CCT2 1
Systematic Name
YIL142W
SGD ID
SGD:S000001404
Aliases
BIN3 1 , TCP2
Feature Type
ORF , Verified
Description
Subunit beta of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo 2 3
Name Description
Chaperonin Containing TCP-1 3
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
CCT2 is located on the left arm of chromosome IX between SSL2 TFIIH subunit and AXL2 plasma membrane protein; dubious ORF YIL142C-A overlaps 5' end of CCT2 on the opposite strand; coding sequence is 1584 nucleotides long with 12 SNPs, one of which causes Thr/Ala polymorphism at residue 136
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Cct2p is 527 amino acids long, longer-lived, high in abundance; acetylated on 3 lysines, ubiquitinylated on 5 lysines, phosphorylated on 14 residues
Length (a.a.)
527
Mol. Weight (Da)
57197.0
Isoelectric Point
5.96
Median Abundance (molecules/cell)
19276 +/- 3563
Half-life (hr)
11.3

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all CCT2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Subunit of the chaperonin-containing T-complex (TriC) that mediates protein folding

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene; reduction of function leads to sensitivity to translation-interfering drugs, such as azetidine-2-carboxylic acid, cycloheximide, hygromycin
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast CCT2 is homologous to human CCT2 and has been used to study mutations that cause Leber congenital amaurosis

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Cct2p interacts physically with proteins involved in meiotic cell cycle; CCT2 interacts genetically with genes involved in mitotic cell cycle

343 total interactions for 298 unique genes

Physical Interactions

  • Affinity Capture-MS: 115
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 6
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-purification: 4
  • Reconstituted Complex: 3

Genetic Interactions

  • Dosage Rescue: 14
  • Negative Genetic: 163
  • Positive Genetic: 16
  • Synthetic Lethality: 7
  • Synthetic Rescue: 6
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
CCT2 promoter is bound by Med2p, Rpd3p, and Stp1p in response to heat; CCT2 transcription is regulated by Gcr1p
Regulators
4
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
27
Additional
27
Reviews
8

Resources