The complete sequence of S. cerevisiae is that of strain
S288C. Therefore, the first step is to be sure that you have
sequenced that strain. Data on other strains are interesting to
remember as notes but cannot result in changing the systematic
sequence edition in the yeast databases. If the strain you sequenced
is different from S288C, you can submit your piece of sequence to
Genbank/EMBL/DDBJ with a clear indication of your strain. Your
sequence will then be linked to the locus in SGD although it will not
be the systematic sequence stored in the database. It is only by
strictly following this principle that we can distinguish sequence
errors that need to be corrected from natural polymorphisms that
should not be inserted in the sequence.
Although nucleotide omission is the most frequent type of sequencing
error, it is not because the protein reported by an author is longer
than the predicted one that it is correct. There are several cases of
real in-frame stop codons verified by direct sequencing of the DNA of
S288C (B. Dujon, personal communication). Therefore, the second
step is to provide the experimental demonstration that
the sequence change you propose is real. Sequencing a PCR reaction
amplified on S288C DNA and covering the region of interest is the best
method.
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