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Complex: Core mediator complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Curated by
IntAct
ComplexAc
CPX-3226
Systematic Name
CSE2:GAL11:MED1:MED2:MED4:MED6:MED7:MED8:MED11:NUT1:NUT2:PGD1:RGR1:ROX3:SIN4:SOH1:SRB2:SRB4:SRB5:SRB6:SRB7
SGD ID
SGD:S000217835
Description
Plays an essential role in gene expression regulation by acting as a bridge between DNA-binding transcription factors and the RNA polymerase II (RNAPII) transcription machinery, serving as a central scaffold within the pre-initiation complex. The Mediator complex is also targeted by sequence-specific, DNA-binding transcription factors and appears to regulate RNAPII at both the initiation and elongation stages of transcription. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and unfolds to an extended conformation and partially surrounds RNAPII specifically interacting with the unphosphorylated form of its C-terminal domain. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. Reversibly associates with the CKM complex (CPX-1853). Mediator lacking the CKM complex has a stimulatory effect on basal transcription. In contrast, Mediator containing the sub-complex represses basal transcription.

Complex Diagram

This diagram displays the protein subunits (blue) of the complex and how they interact with each other. Interactions with other relevant participants such as small molecules (purple), sub-complexes (yellow), and other subunits (red) are also shown. If a participant appears in the diagram with no lines connected to it, then its specific interactions with other participants are currently unknown.

PDB Structure

When available from the Protein Data Bank (PDB), the physical structure of the macromolecular complex is displayed. Click on the image or link for further information about the physical structure.

1YKE 1YKE 1YKH 1YKH 2HZM 2HZM 2HZS 2HZS 3FBI 3FBI 3FBN 3FBN 3J1O 3J1O 3RJ1 3RJ1 4GWP 4GWP

Subunits

This table lists all participants of the complex (proteins, small molecules, nucleic acids, etc.) and their respective stoichiometry. A brief description is also provided for each participant in the table.

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Subunit Description Stoichiometry

Gene Ontology

Gene Ontology (GO) terms that describe the function of a complex, the biological process in which it participates, or its cellular location. Click on a term for its definition and other objects annotated to that term.

Shared Annotations

This diagram displays Gene Ontology terms (green) and subunits (blue) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). The shared GO terms or subunits can be visualized separately using the radio button at the bottom of the graph. Note: No diagram is shown ("No shared annotations") if there are less than 2 shared annotations (either GO terms or subunits of other complexes) between this complex and any other complexes.

References

List of references used specifically to curate the information on this page.

External Identifiers

EMDB: 2634 | EMDB: 5407

Resources

IntAct | ModelArchive | PDB:1YKE | PDB:1YKH | PDB:2HZM | PDB:2HZS | PDB:3FBI | PDB:3FBN | PDB:3J1O | PDB:3RJ1 | PDB:4GWP ... Show all