Yeast Genetics and Molecular Biology 1998
College Park, Maryland
August 1998

Name: Polymenis, Michael
Mailing Address: MGH Cancer Center, Massachusetts General Hospital, Bldg. 149, 13th St., Charlestown, MA 02129, U.S.A.
Email Address: polymeni@helix.mgh.harvard.edu
Phone and Fax numbers: (617)-726-5626, (617)-724-9648

074

An essential and rate limiting function for the G1 cyclin Cln3p in cell proliferation.


Michael Polymenis , Emmett V. Schmidt
MGH Cancer Center, Massachusetts General Hospital, Bldg. 149, 13th St., Charlestown, MA 02129, U.S.A.

Cell proliferation requires coordination of the overall growth of the cell with cell division. In yeast this is achieved by dependence of cell division on the attainment of a critical growth rate at a point called START. We report genetic interactions between the G1 cyclin CLN3 and genes affecting ribosomal availability. Importantly, translational de-repression of Cln3p synthesis is sufficient to overcome the START arrest imposed by limitation of protein synthesis in cdc63 cells. Analysis of proliferating cells that lack CLN3 reveal a rate limiting role for Cln3p in overall cell proliferation when the ribosome content is low. Thus, we define a unique function for Cln3p in coordinating START with overall cellular biosynthesis thereby revealing conditions when cell division, not cell growth, limits cell proliferation.

Return to YGM 1998 Abstract Index