Yeast Genetics and Molecular Biology 1998
College Park, Maryland
August 1998

Name: Ono, Bun-ichiro Ono
Mailing Address: Biotechnology, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, 525-8577, Japan
Email Address: ono@se.ritsumei.ac.jp
Phone and Fax numbers: (81) 77-561-2772, (81) 77-561-2659

056

Structural and functional relationships of ESU1 and GAL11 of the budding yeast Saccharomyces cerevisiae .


Bun-ichiro Ono (1) , Kiyohiro Nakano (1), Vladimir N. Noskov (2), Kazuo Negishi (3)
(1) Biotechnology, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, 525-8577, Japan; (2) Department of Genetics, Sankt Petersburg University, Sankt Petersburg, Russia; (3) Gene Research Center, Okayama University, Okayama 700-8530, Japan

An ORF (1,272 bp), designated ESU1 for enhancement of omnipotent suppression, was identified as the 3'-terminus of the GAL11 gene (3,243 bp) known to code for a component of RNA polymerase II holoenzyme. Contrasting to ESU1 , GAL11 lowered the level of omnipotent suppression. ESU1 was shown to be transcribed using a TATA-like sequence near the center of GAL11 and translated using ATG codons following this TATA-like sequence. Since these ATG codons are in the same reading frame as GAL11 , Esu1p must have the same amino acid sequence, and presumably conformation also, as the C-terminus of Gal11p. Our data suggest that Esu1p activates transcription and that Gal11p antagonizes the action of Esu1p. Based on these findings, we can rationalize the opposite effects of ESU1 and GAL11 on omnipotent suppression by the level of mRNA having immature translational termination signal. Besides such a functional relationship of ESU1 and GAL11 , their structural relationship is also of interest because it may provide a clue for evolution of genes coding for multi-domain peptides. The work was supported partly by HFSPO and partly by the Ministry of Education, Science and Welfare of Japan.

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