Nmt1p catalyzes the co-translational transfer of myristate from CoA to the N-terminal Gly residue of ~12 cellular proteins. The NMT1 gene is essential for vegetative growth. nmt1-181 encodes a mutant enzyme with a reduced affinity for myristoylCoA, produces global defects in protein N-myristoylation, and results in growth arrest at or above 30oC. nmt1-181 cells can be rescued up to 33oC by removing inositol from synthetic media. MyristoylCoA is produced by the *alpha*6 *beta*6 fatty acid synthase (Fas). FAS1 and FAS2 encode the *beta* and *alpha* subunits of Fas. Since expression of FAS1 and FAS2is regulated by inositol, we investigated their transcription using FAS1-lacZ and FAS2 lacZ reporter plasmids in isogenic NMT1 and nmt1-181 strains. Transcription of FAS1 in NMT1 cells decreased in the presence or absence of inositol as incubation temperature was raised from 24 to 33oC. Expression of FAS2 in NMT1 cells also decreased in the presence of inositol but did not change over this temperature range when inositol was absent. When assayed at 30oC, nmt1-181 cells demonstrated significantly higher levels of FAS1 and FAS2 transcription in the absence of inositol compared to isogenic NMT1 cells. Furthermore, nmt1-181 cells show increased expression of INO2 in the presence of inositol and decreased expression of OPI1 in the absence of inositol. We hypothesize that cellular N-myristoylproteins affect expression of FAS genes through their regulation of INO2 and OPI1 expression.