Proper distribution of duplicated nuclei between mother and daughter cells requires the concerted action of a multitude of proteins. Mutations in the genes involved in this process, frequently cause pleiotropic defects due to the high degree of interdependencies between the corresponding proteins. Deletion of N1264, a novel gene identified during systematic sequencing causes several severe deficiencies in haploid segregants of two strains. Here, the slow growing cells are frequently multinucleate or anucleate. Morphological abnormalities like elongation or irregular shape of cells, are observed to different degrees in the two strain backgrounds. The formation of multibudded cells and a reduced ability to undergo cytokinesis are additional consequences of N1264 loss. The wildtype phenotype was restored upon transformation with the wildtype gene on a single copy plasmid. In a third strain background , deletion of N1264 is lethal in haploid segregants. We constructed a N1264-GFP(green fluorescent protein) fusion by insertion of a PCR generated GFP-marker double cassette at the 3' end of the gene's genomic locus. The resulting strain is phenotypically indistinguishable from the wildtype. Preliminary studies on the localization of the fusion protein revealed a few distinct fluorescent spots per cell.