Abstract #70

The histone deacetylase Rdp3p coordinates induced and repressed expression changes in yeast cells responding to stress. Adriana Alejandro-Osorio¹, Dominic Porcaro², Audrey Gasch^2,3. 1) Department of Biomolecular Chemistry, University of Wisconsin- Madison, Madison, WI; 2) Department of Genetics, University of Wisconsin-Madison, Madison, WI; 3) Genome Center of Wisconsin.
   Yeast cells respond to stress by mounting a large gene expression program called the environmental stress response (ESR), which consists of ~600 genes that are repressed and ~300 genes that become induced in response to diverse stresses. How the cell coordinates these ~900 gene expression changes is not understood. We find that the histone deacetylase Rdp3p is required for proper expression of the ESR genes, including genes induced and repressed in the response. Different subsets of ESR genes show different dependencies on Rdp3p: the repression of one class of ESR genes is largely dependent on Rdp3p, while the repression of another class of genes encoding ribosomal proteins is only partially dependent on Rdp3p under certain conditions. Interestingly, genes induced as part of the ESR require Rdp3p activity for full induction in response to stress and also for suppression in the absence of stress, suggesting that Rdp3p plays reciprocal regulatory roles under different conditions. Computational analysis of our data coupled with other large-scale datasets implicates transcriptional regulators that function with or act independently of Rdp3p. Together, these data allow us to construct a model transcriptional regulatory network that coordinates expression of the ESR genes.

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