Browse Workshop Speaker & Poster Abstracts

XXIInd International Conference on Yeast Genetics and Molecular Biology: Workshop Speaker & Poster Abstracts

Workshop & Poster Abstracts Topic 1 - Evolutionary and comparative genomics

1-1

The MAL locus of Hansenula polymorpha is organized similarly to respective gene clusters of other yeasts and hides a divergent promoter with a biotechnological potential.
Tiina Alamäe, Katrin Viigand, Birgit Rätsep, Kersti Tammus
Department of Genetics, University of Tartu, 23 Riia Str, Tartu, 51010, Estonia

1-2

Complete DNA sequences of the mitochondrial genomes of the pathogenic yeasts Candida orthopsilosis and C. metapsilosis.
Peter Kosa (1), Matus Valach (1), Lubomir Tomaska (2), Kenneth H. Wolfe (3), Jozef Nosek (1)
(1) Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak republic; (2) Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak republic; (3) Department of Genetics, University of Dublin, Trinity College, Dublin, Ireland

1-3

General structure of subtelomeres is conserved in hemiascomycetes.
Cecile Fairhead, Bernard Dujon
Struct and Dynamics of Genomes, Institut Pasteur, 25 rue du Dr Roux, Paris, 75015, France

1-4

Convergent evolution of the mitochondrial protein translocation machinery.
Andrew Perry, Joanne Hulett, Nickie Chan, Peter Walsh, Vladimir Likic, Paul Gooley, Trevor Lithgow
Biochem. & Mol. Biol., University of Melbourne, Flemington Road, Parkville, 3010, Australia

1-5

Evolution of petite-positivity/negativity in yeasts.
Veronika Fekete (1), Silvia Poláková (1), Marika Čierna (1), Jure Piskur (2), Pavol Sulo (1)
(1) Department of Biochemistry, Comenius University, Mlynska Dolina, Bratislava, 842 15, Slovakia; (2) Cell and Organism Biology, Lunds University, 22362 Lund, Sweden

1-6

Genome sequence survey and design of microarrays for Candida parapsilosis.
Tristan Rossignol, Mary E. Logue, Geraldine Butler
Department of Biochemistry, Conway Institute, UCD, Belfield, Dublin, Dublin 4, Ireland

1-7

The Yarrowia lipolytica transposon content.
Serge Casaregola (1), Cécile Neuvéglise (1), Christophe Ozanne (1), Patrick Wincker (2), Claude Gaillardin (1)
(1) Microbiol. et Genet. Molec., INRA/CNRS, INA-PG, Thiverval-Grignon, 78850, France; (2) Genoscope, Centre National de Séquençage, Evry, France

1-8

Fungal phylogenomics with mitochondrial and nuclear genes: are fission yeasts Archiascomycota?
Yu Liu (1), Naiara Rodriguez-Ezpeleta (2), Henner Brinkmann (2), Herve Philippe (2), B. Franz Lang (2)
(1) Department de Biochemie, Universite de Montreal, Montreal, H3T1J4, Canada; (2) Department de Biochemie, Universite de Montreal, Edouard Montpetit, Montreal, H3T1J4, Canada

1-9

Comparative analysis of genomes of cultured Saccharomyces yeasts.
Elena S. Naumova (1), Yulia V. Ivannikova (1), Nikolai N. Martynenko (2), Gennadi I. Naumov (1)
(1) Lab. of yeast genetics, GosNIIgenetika, I-Dorozhnyi,1, Moscow, 117545, Russia; (2) Moscow State University of Food Production, Moscow 125080, Russia

1-10

Phylogenomics of Fungi: Nuclearia is the deepest fungal divergence.
Emma Steenkamp (1), Yu Liu (2), B. Franz Lang (3)
(1) Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, 0001, South Africa; (2) Biochimie, Universite de Montreal, 2900, E. Montpetit, Montreal, H3C 3J7, Canada; (3) Canadian Institute for Advanced Research, Centre Robert Cedergren, Départment de Biochemie, Université de Montréal, 2900 Boulevard Edouard Montpetit, Montréal, Québec, H3T 1J4, Canada

1-11

Brettanomyces/Dekkera, a novel model organism to elucidate yeast evolution and comparative genomics.
Jure Piskur (1), Jouzas Siurkus (2), Mikael R. Andersen (2), Gloria Joergensen (2), Ela Rozpedowska (1), Silvia Polakova (3), Anamaria Merico (4), Ileana Vigentini (4), Pavol Sulo (5), Dorte Joerck-Ramberg (2), Ken Wolfe (6), Concetta Compagno (4)
(1) Cell and Organism Biology, Lund University, Soelvegatan 35, Lund, 22362, Sweden; (2) Technical University of Denmark; (3) Technical University of Denamrk and Comenius University, Bratislava; (4) University of Milan; (5) Comenius University, Bratislava; (6) Trinity College, Dublin

1-12

The mitochondrial DNA of lager brewing strains.
Sandra Rainieri, Yukiko Kodama, Yoshihiro Nakao, Wataru Miki
Institute for Advanced Technol, Suntory Research Center, 1 1 1 Wakayamadai, Osaka, 618 8503, Japan

1-13

Chromosomal structure of lager brewing yeasts assessed with a newly developed lager brewing yeast DNA microarray.
Yukiko Kodama (1), Yoshihiro Nakao (1), Tomoko Fujimura (1), Norihisa Nakamura (1), Wataru Miki (1), Takehiko Ito (2), Katsuhiko Shirahige (3), Toshihiko Ashikari (4)
(1) Inst. for Advanced Technology, Suntory Ltd., 1-1-1, Wakayamadai, Osaka, 618-8503, Japan; (2) Mitsubishi Research Institute, INC., 2-3-6, Otemachi, Chiyoda-ku, Tokyo, 100-8141 Japan; (3) Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan; (4) Florigene, 16 Gipps Street Collingwood VIC 3066, Australia

1-14

Why does Kluyveromyces lactis not grow anaerobically?
Ishtar Snoek (1), Yde Steensma (2)
(1) Wassenaarseweg 64, 2333 AL Leiden, Netherlands; (2) IBL, yeastgenetics, Leiden university, Wassenaarseweg 64, Leiden, 2333 AL, Netherlands

1-15

Looking for promiscuous DNA in hemiascomycetous genomes.
Odile Ozier-Kalogeropoulos (1), Christine Sacerdot (1), Serge Casaregola (2), Fredj Tekaia (1), Norio Gunge (3), Kohsai Fukuda (3), Bernard Dujon (1)
(1) Struct. et Dyn. des Genomes, Institut Pasteur, 25 rue du Dr Roux, Paris, 75724, France; (2) Laboratoire de Microbiologie et Genetique Moleculaire, INRA UMR1238, CNRS/INA-PG UMR 2585, 78850 Thiverval-Grignon, France; (3) Sojo-University, Ikeda 4-22-1, Kumamoto, 860-0082, Japan

1-16

Transcriptional analysis of coexisting genomes in interspecific hybrids of Saccharomyces cerevisiae and Saccharomyces bayanus.
Duccio Cavalieri (1), Julia Oh (2), Christine Queitsch (3), Christian Landry (4)
(1) Department of Pharmacology, University of Florence, Viale Pieraccini 6, Florence, 50139, Italy; (2) Department of Biological Sciences, Stanford University, Stanford CA, 94305 USA; (3) Bauer Center for Genomics Research, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138 USA; (4) Organismic and Evolutionary Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138 USA

1-17

Microarray karyotyping of commercial wine yeast strains reveals shared, as well as unique, genomic signatures.
Barbara Dunn (1), R. Paul Levine (2), Gavin Sherlock (1)
(1) Dept of Genetics, Stanford University Med Ctr, 300 Pasteur Ave, Stanford, CA, 94305, USA; (2) Dept of Biological Sciences, Stanford University, Stanford, CA 94305 USA

1-18

Evolutionary conservation and divergence of fungal promoter sequences.
Riccarda Rischatsch, Sylvia Voegeli, Peter Philippsen
Applied Microbiology, Biozentrum Basel, Klingelbergstr. 50, Basel, CH-4056, Switzerland

1-19

Exploring genome plasticity and adaptive evolution in natural and de novo laboratory hybrid yeast species.
Barbara Dunn (1), Giani Liti (2), Ed Louis (2), R. Frank Rosenzweig (3), Gavin Sherlock (1)
(1) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA; (2) Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH United Kingdom; (3) Division of Biological Sciences, The University of Montana, 32 Campus Drive #4824, Missoula MT, USA

Workshop & Poster Abstracts Topic 2 - Biotechnology and ecology of yeasts

2-1

Identification of genes contributing to a 'High Nitrogen Efficiency' (HNE) phenotype in yeast.
Jennie Gardner (1), Miguel de Barros Lopes (2), Vladimir Jiranek (1)
(1) School of Agriculture and Wine, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia; (2) School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, GPO Box 2471, Adelaide, SA, 5001, Australia

2-2

Adaptive evolution in enhancing wine yeast.
Colin McBryde (1), Miguel de Barros Lopes (2), Vladimir Jiranek (1)
(1) School of Agriculture and Wine, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia; (2) School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, GPO Box 2471, Adelaide, SA, 5001, Australia

2-3

Genetic and environmental control of sexual development in hemiascomycetous yeast Hansenula polymorpha CBS4732.
Kancho Lahchev
Genetics of Microorganisms, Institute of Microbiology, Acad.G.Bontchev 26, Sofia, 1113, Bulgaria

2-4

S. cerevisiae transcriptional response to the presence of a competing yeast in wine fermentation.
Oreto Antúnez, José E. Pérez-Ortín
Bioquímica y Biología Molecula, Universitat de València, Dr. Moliner 50, Burjassot, E46100, Spain

2-5

Genetic stability of wine yeast and their hybrids.
Alina Kunicka, Katarzyna Rajkowska
Inst. Ferm. Technol. Microbiol, Technical University of Lodz, Wolczanska, Lodz, 90-924, Poland

2-6

Yeast extracts with increased RNA content.
Peter Dekker, Carlos Ribeiro
R&D, DSM Food-Specialties, PO Box 1, Delft, 2600 MA, The Netherlands

2-7

Analysis of global gene expression profiles of a sake yeast during ethanol fermentation.
Hong Wu, Hitoshi Shimoi
Genetic Engineering Division, Nat. Res. Institute of Brewing, 3-7-1, Kagamiyama, Higashihiroshima, 739-0046, Japan

2-8

Genetic engineering of a yeast strain fermenting both pentose sugars, L-arabinose and D-xylose.
Beate Wiedemann, Eckhard Boles
Institut fuer Mikrobiologie, J. W. Goethe-Universitaet, Marie-Curie-Str. 9, Frankfurt / Main, 60439, Germany

2-9

High-level secretion of recombinant proteins from S. cerevisiae by co-expression of genes from 2-micron vectors.
Chris Finnis, Gill Shuttleworth, Darrell Sleep
Molecular Biology Department, Delta Biotechnology Ltd., 59 Castle Boulevard, Nottingham, NG7 1FD, United Kingdom

2-10

Characterisation of yeast genes involved in flavour determination in wine.
Kate Howell (1), Mathias Klein (2), Jan H Swiegers (1), Yoji Hayasaka (1), Gordon M Elsey (3), Graham H Fleet (4), Peter B Høj (5), Isak S Pretorius (1), Paul Chambers (1), Miguel A de Barros Lopes (6)
(1) Biosciences Group, Australian Wine Research Inst., PO Box 197, Glen Osmond, Adel, SA, 5064, Australia; (2) Present address, Department of Biotechnology, Aachen University of Technology (RWTH), D-52074 Aachen, Germany; (3) School of Chemistry, Physics and Earth Sciences, Flinders University, GPO Box 2100, Adelaide SA 5100, Australia; (4) Food Science and Technology, School of Chemical Engineering and Industrial Chemistry, University of New South Wales, Sydney NSW 2052, Australia; (5) Present address:, The Australian Research Council, GPO Box 2702 Canberra ACT 2601 Australia; (6) Present address, School of Pharmacy and Medical Sciences, University of South Australia, GPO Box 2471, Adelaide SA 5001, Australia

2-11

Cloning and partial sequencing of the NADPH-cytochrome P450 reductase gene of Candida bombicola.
Inge N.A. Van Bogaert, Sofie De Maeseneire, Erick J. Vandamme, Wim Soetaert
Lab. Ind. Microb. & Biocat., Ghent University, Coupure Links 653, Ghent, 9000, Belgium

2-12

Screening the probiotic potential of food-bourne yeast strains.
Nicolas Pagé, Dominique de Maleprade, Sophie Meier, Olivia Morandi, Fanny Scalfo, Josette Sidoti, Christof Gysler
Food Science, Nestlé Research Centre, Vers-chez-les-blanc, Lausanne 26, CH-1000, Switzerland

2-13

Development of yeast-based fluorogenic biosensors for detection of DNA-damaging agents in environmental samples.
Susanna Boronat, Benjamín Piña
Molecular and Cellular Biology, IBMB-CSIC, Jordi Girona, 18, Barcelona, 08034, Spain

2-14

Propagation and Fermentation Trials with High Gravity Resistant Mutant Lager Yeast Strains.
Linda Mulder (1), Anne Huuskonen (2), Wim Geurts (1), John Londesborough (2), Michael Walsh (1)
(1) Brewing Science & Technology, Heineken Technical Services, Burg. Smeetsweg 1, Zoeterwoude, 2382 PH, the Netherlands; (2) Bioprocess Physiology, VTT Biotechnology, P.O.Box 1500, FIN-02044 VTT, Finland

2-15

An evolved recombinant plasmid-carrying Saccharomyces cerevisiae strain presenting an improved lactose consumption phenotype.
Pedro Guimarães, Nelson Lima, José A. Teixeira, Lucília Domingues
Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, Braga, 4715-057, Portugal

2-16

Analysis of the genome-wide gene expression of heterologous protein producing fission yeast strains.
Hideki Tohda, Hiromichi Kumagai, Yuko Giga-Hama
ASPEX Division, Asahi Glass Co., Ltd., Kanagawa-ku, Yokohama, 221-8755, Japan

2-17

Differentiation of dairy yeast Kluyveromyces lactis var. lactis and its closest wild relatives from European population 'krassilnikovii'.
Gennadi I. Naumov, Nataliya N. Sukhotina, Elena S. Naumova
Lab. of yeast genetics, GosNIIgenetika, I-Dorozhnyi, 1, Moscow, 117545, Russia

2-18

Construction of Lager brewing yeast DNA microarray and genome wide gene expression analysis during the lager brewing process.
Yoshihiro Nakao (1), Yukiko Kodama (1), Tomoko Fujimura (1), Wataru Miki (1), Takehiko Ito (2), Katsuhiko Shirahige (3), Toshihiko Ashikari (4)
(1) Inst. for Advanced Technology, SUNTORY LIMITED, 1-1-1 Wakayamadai, Osaka, 618-8503, Japan; (2) Mitsubishi Research Institute, INC., 2-3-6, Otemachi, Chiyoda-ku, Tokyo, 100-8141 Japan; (3) Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku, Yokohama, 226-8501, Japan; (4) Florigene, 16 Gipps Street Collingwood VIC 3066, Australia

2-19

Global analysis of gene expression of the wine yeast EC1118 during rehydration and following inoculation.
Bruno Blondin, Tristan Rossignol, Julien Storai, Olivier Postaire
Microbiology, INRA - Agro.M, 1, place Viala, Montpellier, 34060, France

2-20

A homozygous diploid subset of commercial wine yeast strains.
Heather Brown, Keith Richards, Soon Lee, John Bradbury, Rod Dunbar, Richard Gardner
School of Biological Sciences, University of Auckland, 5 Symonds Street, Auckland, New Zealand

2-21

Efficient gene deletion systems in the Schizosaccharomyces pombe.
Kyotaro Hirashima (1), Tomoko Iwaki (2), Hideki Tohda (1), Hiromichi Kumagai (1), Yuko Giga-Hama (1), Kaoru Takegawa (2)
(1) ASPEX Division, Asahi Glass Co., Ltd., 1150 Hazawa Kanagawa, Yokohama, 221-8755, Japan; (2) Kagawa Univ. Mikicho Kagawa Japan, 761-0795

2-22

Characterization of the starch binding domain of Lipomyces kononenkoae alpha-amylase LKA1.
Ricardo Cordero Otero (1), Sakkie Pretorius (2), Nivetha Ramachandran (1)
(1) Inst. for Wine Biotechnol., University of Stellenbosch, Victoria St., Stellenbosch, 7600, South Africa; (2) IWBT, and The Australian Wine Research Institute, Adelaide, Australia

2-23

Monitoring the transcriptome and the proteome of the wine yeast EC1118 during alcoholic fermentation.
Tristan Rossignol (1), Dominique kobi (2), Laurence Gutfreund (2), Bruno Blondin (1)
(1) Microbiology, UMR SPO INRA-Agrgo.M, 1, place Viala, Montpellier, 34060, Montpellier, France; (2) Tepral Kronenbourg, 68, Route d'Oberhausbergen, 67037 Strasbourg

2-24

A universal and comparative heterologous expression system for fungal epoxide hydrolase encoding genes.
Michel Labuschagne (1), Catherine Madzak (2), Jacobus Albertyn (1)
(1) Biotechnology, University of the Free State, Nelson Mandela Ave., Bloemfontein, 9300, South Africa; (2) UMR Microbiologie et Génétique Moléculaire INRA - CNRS - INAPG, CBAI, 78850 Thiverval Grignon, France

2-25

Isolation and heterologous expression of an epoxide hydrolase from Rhodotorula mucilaginosa.
Michel Labuschagne (1), Catherine Madzak (2), Jacobus Albertyn (1)
(1) Biotechnology, University of the Free State, Nelson Mandela Ave., Bloemfontein, 9300, South Africa; (2) UMR Microbiologie et Génétique Moléculaire INRA - CNRS - INAPG, CBAI, 78850 Thiverval Grignon, France.

2-26

Lipomyces kononenkoae LKA1 alpha-amylase Gln⁴⁴³ is critical for enzyme activity.
Ricardo Cordero Otero (1), Sakkie Pretorius (2), Nivetha Ramachandran (1)
(1) Inst. for Wine Biotechnol., University of Stellenbosch, Victoria St., Stellenbosch, 7600, South Africa; (2) IWBT; The Australian Wine Research Institute, Adelaide, Australia

2-27

Role of interference competition in the maintenance of diversity in yeast Saccharomyces cerevisiae.
Dominika M. Wloch-Salamon, J. Arjan G. M de Visser, Rolf F. Hoekstra
Laboratory of Genetics, WAGENINGEN UNIVERSITY, Arboretumlaan 4, Wageningen, 6703 BD, Netherlands

2-28

Towards cancer vaccine made from recombinant yeast cells.
Walid Omara (1), Yaroslav Terentjev (2), Steve Oliver (2), Lubomira Stateva (2)
(1) Faculty of Life Sciences, The Michael Smith Building, Oxford Road, The University of manchester, Manchester, Uk. , M13 9PT; (2) Biochemistry, The university of Manchester, Oxford Road, Manchester, M13 9PT, UK

2-29

Polymorphism of rDNA fragments identified by PCR-RFLP method in yeast isolates from cheeses.
Malgorzata Robak, Xymena Polomska, Michal Piegza, Wojciech Barszczewski, Maria Wojtatowicz
Biotechnol.&Food Microbiol., Agricultural University, Norwida 25, Wroclaw, 50-375, Poland

2-30

QTL Analysis of brewing performances in bottom fermenting yeast.
Osamu Kobayashi (1), Shoko Nakagawa (1), Shigehito Ikushima (1), Kaori Hashimoto (1), Shinji Kawasaki (2), Keiko Tanaka (1)
(1) Central Labs for Frontier Tech, Kirin Brewery Co., Ltd., 1-13-5, Fukuura, Yokohama-shi, 236-0004, Japan; (2) National Inst Agrobiol Science, 2-1-2, Kan-nondai, Tsukuba-shi, 305-8602, Japan

2-31

Pathways of GABA assimilation and effect on Saccharomyces cerevisiae metabolism during wine fermentation.
Benoît Bach, Carole Camarasa, Sylvie Dequin
UMR SPO-Microbiologie, INRA, 2 place Pierre Viala, Montpellier, 34060, France

2-32

Growth, substrate consumption and metabolite formation during aerobic batch cultivations of Kluyveromyces marxianus CBS 6556 on different carbon sources.
Gustavo Graciano Fonseca (1), Nuno Miguel Barbosa de Carvalho (1), Andreas Karoly Gombert (2)
(1) Chemical Engineering, University of Sao Paulo, C.P. 61548, Sao Paulo, SP, 05424-970, Brazil; (2) University of Sao Paulo, Department of Chemical Engineering, C.P. 61548, 05424-970, Sao Paulo-SP, Brazil

2-33

Engineering NADH oxidation for decreasing ethanol yield from glucose in Saccharomyces cerevisiae.
Stephanie Heux, Jean Marie Sablayrolles, Rémy Cachon, Sylvie Dequin
UMR SPO-Microbiologie, INRA, 2 place Pierre Viala, Montpellier, 34060, France

2-34

Human thyroid peroxidase is retained and aggregated during expression in Pichia pastoris and Saccharomyces cerevisiae.
Pavla Vasicova, Jiri Hasek, Ivana Malcova-Janatova
Lab.Cell Reproduction, Institute of Microbiology ASCR, Videnska 1083, Prague 4, 14220, Czech Republic

2-35

Generation of gene disruption cassettes with long flanking regions via fusion PCR.
Andreas Karoly Gombert (1), Björn Johansson (2), Nelson Lima (3), José Teixeira (3), Lucília Domingues (3)
(1) Chemical Engineering, University of Sao Paulo, C.P. 61548, Sao Paulo, SP, 05424-970, Brazil; (2) University of Minho, Department of Biology, Braga, Portugal; (3) University of Minho, Department of Biological Engineering, Braga, Portugal

2-36

Genetic structure of vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis.
Dorit Schuller, Paula Sampaio, Célia Pais, Margarida Casal
Departamento de Biologia, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal

2-37

Dissemination and survival of commercial wine yeast in the vineyard: a large-scale, three years study.
Eva Valero (1), Dorit Schuller (2), Brigitte Cambon (1), Margarida Casal (2), Sylvie Dequin (1)
(1) Institut National de la Recherche Agronomique, UMR Sciences pour l'Oenologie, Place Viala, Montpellier, 34060, France; (2) Departamento de Biologia, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal

2-38

Use of a yeast display library of human antibody recognition sequences to develop detection assays for potential cancer biomarkers.
Barbara Garvik, Nicole Urban, Nathalie Scholler
Public Health Sciences, Fred Hutchinson Cancer Rsh Ctr, 1100 Fairview Ave. N, Seattle, WA, 98109, USA

2-39

Yeast Saccharomyces cerevisiae and Pichia pastoris strains - producers of intracellular bovine interferon-gamma.
Anastasia Gradoboeva (1), Mikhail Smirnov (2), Marina Padkina (1)
(1) Laboratory of biochemical genetics, Biological Institute, Oranienbaumskoe sh., Saint Petersburg, 198904, Russian Federation; (2) Bolshoi pr. P.S.,29-A, 197198, Saint-Petersburg, Russia

2-40

In vivo gene shuffling of hydroxynitrile lyases.
Hélène Fouché (1), Katja Prutej (2), Peter Remler (2), Helmut Schwab (2), Rhona H. Borts (3), Kathleen N. Smith (1)
(1) Eucodis France, Faculté de Médecine Necker, 156 rue de Vaugirard, 75730 Paris Cedex 15, France; (2) Angewandte Biokatalyse Kompetenzzentrum GmbH, Petersgasse 14, A-8010 Graz, Austria; (3) University of Leicester, University Rd, Leicester LE1 7RH, UK

2-41

Effect of low temperature fermentation on yeast nitrogen metabolism.

Gemma Beltran (1), Maite Novo (1), Nicolas Rozès (1), Albert Mas (1), Ricardo Cordero (2), José M. Guillamón (1)

Workshop & Poster Abstracts Topic 3 - Morphogenesis, cytoskeleton, and cell wall

3-1

Polymorphism of the cell wall protein ccw7 encoding gene HSP150 in film-forming and sedimenting wine yeast strains.
Mónika Kovács (1), Igor Stuparevič (2), Vladimir Mrsa (2), Anna Maráz (1)
(1) Department of Microbiology and Biotechnology, Faculty of Food Science, Corvinus University of Budapest, Hungary; (2) Department of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia

3-2

The role of endocytosis during sporulation in Saccharomyces cerevisiae.
Masayo Morishita (1), JoAnne Engebrecht (2)
(1) Molecular & Cellular Biology, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA; (2) Molecular & Cellular Biology, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA

3-3

Systematic analysis of yeast cortical tag proteins for functionality and localization.
Anne-Brit Obermayer, Inken Wollenweber, Hans-Ulrich Mösch
Dept. of Biology-Genetics, Philipps-University, Karl-von-Frisch-Str., Marburg, D-35032, Germany

3-4

Structure and function of Awa1p, which is responsible for cell surface hydrophobicity of sake yeasts.
Masashi Shimizu, Kiyoshi Ito, Hitoshi Shimoi
Genetic Engineering Division, Nat. Res. Institute of Brewing, 3-7-1, Kagamiyama, Higashihiroshima, 739-0046, Japan

3-5

Study of the interaction of ubiquitin-protein ligase Rsp5p with actin cytoskeleton proteins Bee1p and Lsb1p in S. cerevisiae.
Marta Stawiecka (1), Teresa Zoladek (1)
(1) Department of Genetics, IBB PAS, Warsaw, Poland

3-6

Role of Nrg1 in the regulation of morphogenesis in Candida albicans.
Silvia Argimon, Claire L. Russell, Abigail Mavor, Claire Manson, Susan MacKaskill, Alistair J.P. Brown
Aberdeen Fungal Group, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

3-7

Interaction of Chs4p and Chs3p mediates Chitin synthase III activation in Saccharomyces cerevisiae.
Abigail Reyes, Maria Sanz, Rosario Valle, Angel Duran, Cesar Roncero
Microbiologia y Genetica, CSIC/Universidad de Salamanca, Avda. Campo Charro, Salamanca, 37007, Salamanca, Spain

3-8

Methylglyoxal, a metabolite derived from glycolysis, causes growth inhibition through actin-depolarization.
Kazuhiro Maeta, Shingo Izawa, Yoshiharu Inoue
Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan

3-9

CaMOB2, CaTAO3, CaHYM1 and CaKIC1 are required for hyphal morphogenesis in Candida albicans.
Yunkyung Song, Soyeon Lee, Seon Ah Cheon, Jeong-Yoon Kim
Department of Microbiology, Chungnam National University, Yuseong-gu, Daejeon, 305-764, Korea

3-10

Regulation of the CRH1 expression in the compensatory response to cell wall damage in Saccharomyces cerevisiae.
Estefania Rodriguez, Raul Garcia, Clara Bermejo, Patricia Arias, Jose Manuel Rodriguez-Peña, Carmen Rivas, Cesar Nombela, Javier Arroyo
Microbiology, Faculty of Pharmacy, U.C.M., Pza.Ramon y Cajal, Madrid, 28040, Spain

3-11

Morphology and physiology of colonies of wild Saccharomyces cerevisiae strains.
Vratislav Stovicek (1), Libuse Vachova (2), Zdena Palkova (1)
(1) Dept. Genetics and Microbiol., Charles University, Vinicna 5, Prague, 12844, Czech Republic; (2) Institute of Microbiology, Academy of Sciences, Videnska 1083, Prague, 14220, Czech Republic

3-12

Cell polarity in the fission yeast ras1 mutant.
Alena Pichova (1), Karel Sigler (1), Kenji Tanaka (2), Masayuki Yamamoto (3)
(1) Cell and Mol. Microbiology, Institute of Microbiology, Videnska 1083, Praha, CZ 142 20, Czech Republic; (2) Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8, Kazusakamatari, Kisarazu-shi, Chiba 292-0818, Japan; (3) Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

3-13

Nitrogen limitation leads to evolution of pseudo-invasive growth in Saccharomyces cerevisiae but not elimination of the Crabtree effect.
Renata Usaite (1), Jens Nielsen (1), Birgitte Regenberg (2)
(1) Center for Microbial Biotechnology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark; (2) Biozentrum, Universität Frankfurt am Main, Marie-Curie-Str., Frankfurt am Main, 60439, Germany

3-14

Spore shaping in yeast.
Aleksander Benjak, Michael Knop
Cell Biology and Biophysics, EMBL, Meyerhofstr. 1, Heidelberg, 69117, Germany

3-15

Altered morphogenesis of Saccharomyces cerevisiae isw2 mutant alpha cells is caused by the autocrine activation of the mating pathway.
Petra Trachtulcova, Ivana Frydlova, Ivana Malcova-Janatova, Jiri Hasek
Lab.Cell Reproduction, Institute of Microbiology ASCR, Videnska 1083, Prague 4, 14220, Czech Republic

3-16

Phenotypic switching of Saccharomyces cerevisiae wild strain.
Jaroslav Lev (1), Liba Vachova (2), Zdena Palkova (1)
(1) Dep. of Genetics, Microbiology, Faculty of Sciences, Vinicna 5, Praha, 128 43, Czech Republic; (2) Institute of Microbiology, Academy of Sciences, Videnska 1083, Praha 4, Czech Republic

3-17

Analysis of the different colony morphotypes of the feral strains of Saccharomyces cerevisiae.
Martin Kuthan, Zdena Palkova
Dept. Genetics and Microbiol., Charles University, Vinicna 5, Prague, 12844, Czech Republic

3-18

Cellular function of the SPB proteins Cnm67 and Spc72 in Saccaromyces cerevisiae and the filamentous fungus Ashbya gossypii.
Claudia Birrer, Tineke van den Hoorn, Peter Philippsen, Dominic Hoepfner
Applied Microbiology, Biozentrum, University Basel, Klingelbergstr. 50, Basel, 4056, Switzerland

3-19

Function of homologs of yeast dynamins in the filamentous ascomycete Ashbya gossypii.
Ivan Schlatter, Peter Philippsen, Dominic Hoepfner
Applied Microbiology, Biozentrum, University Basel, Klingelbergstr. 50, Basel, 4056, Switzerland

3-20

The dynamic cytoskeleton of the filamentous fungus Ashbya gossypii.
Philippe Laissue (1), Hans-Peter Schmitz (2), Philipp Knechtle (1), Peter Philippsen (1)
(1) Angewandte Mikrobiologie, Biozentrum, Universitaet Basel, Klingelbergstr.50/70, Basel, CH-4056, Switzerland; (2) Universität Osnabrück , AG Genetik, Barbarastr. 11 , D-49076 Osnabrück, Germany

3-21

Cdc42 and Rac1 have fundamentally different roles in Candida albicans development.
Martine Bassilana, Robert Arkowitz
Inst.Signaling,Dev.Bio.&Cancer, CNRS UMR6543,Fac. des Sciences, Parc Valrose, Nice, 06108, France

3-22

Regulation of the Cdc42 GTPase module during cell fusion in S. cerevisiae.
Sophie Barale (1), Derek McCusker (2), Robert A. Arkowitz (1)
(1) Inst. Sig., Dev. Bio, & Cancer, CNRS UMR 6543, Parc Valrose, Nice, 06108, France; (2) Dept of Biology, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, California 95064, USA

3-23

Characterization of two putative Candida albicans zinc finger transcription factors possibly involved in regulating cell wall function.
Tobias Schwarzmueller (1), Alisina Sarabi (1), Steffen Rupp (2), Karl Kuchler (1)
(1) Medical Biochemistry, Medical University of Vienna, Dr. Bohr Gasse 9/2, Wien, 1030, Austria; (2) Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB, Nobelstraße 12, D-70569 Stuttgart, Germany

Workshop & Poster Abstracts Topic 4 - New developments in methodologies, rational drug design

4-1

Yeast models to study sulfadrug resistance in pathogens.
Ian Macreadie (1), Peter Iliades (1), Steven Meshnick (2)
(1) Health Sciences and Nutrition, CSIRO, 343 Royal Parade, Parkville, Vi, 3052, Australia; (2) Department of Microbiology, University of North Carolina, Chapel Hill, NC, USA

4-2

Optimizing of a sensitive method for detection of hazardous agents in the environment.
Ekaterina Peycheva, George Miloshev
Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev, bl.21, Sofia, 1113, Bulgaria

4-3

The improved method for identification of drug-target molecules under yeast inducible expression system.
Kaoru Ogawa, Makiyo Hinata, Toru Arai, Kappei Tsukahara, Junro Kuromitsu, Takeshi Nagasu
Tsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3, Tokodai, Tsukuba, 300-2635, Japan

4-4

McRAPD - a new approach to rapid and accurate identification of pathogenic yeast species.
Vladislav Raclavsky (1), Jitka Trtkova (1), Lucia Smilkova (1), Anna Konieczna (1), Radek Plachy (2), Petr Hamal (3)
(1) Dept. of Biology, Palacky University, Hnevotinska 3, Olomouc, 775 15, Czech Republic; (2) Haemato-Oncology Ward, University Hospital, I.P.Pavlova 6, 775 20 Olomouc, Czech Republic; (3) Dept. of Microbiology, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic

4-5

Manipulation of chromosomes in Saccharomyces cerevisiae : Construction of one-gene-chromosome and conditional chromosome splitting with homing endonuclease PI-SceI.
Kazuo Yamagishi, Minetaka Sugiyama, Yoshinobu Kaneko, Satoshi Harashima
Dept. Biotechnology, Osaka Univ., 2-1 Yamadaoka, Suita, 565-0871, Japan

4-6

FlAsH labelling in living yeast cells.
Christian A. Wurm, Martin Andresen, Stefan Jakobs
Dept. NanoBiophotonics, MPI Biophysical Chemistry, Am Fassberg 11, Goettingen, 37077, Germany

4-7

Successive truncation of yeast genome toward the construction of 'minimal genome' yeast.
Kiriko Murakami (1), Yuki Ito (1), Eriko Tao (1), Minetaka Sugiyama (2), Yoshinobu Kaneko (2), Satoshi Harashima (2), Takahiro Sumiya (3), Atsushi Nakamura (3), Masafumi Nishizawa (1)
(1) Dept. Microbiol. Immunol., Keio Univ. School of Medicine, Shinjuku, Tokyo, 160-8582, Japan; (2) Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan; (3) RIISE, Hiroshima University, Higashi-Hiroshima, Japan

4-8

Improvements of genome-wide screening system for identification of drug target pathway using yeast deletion set.
Makiyo Hinata (1), Hirotaka Matsuzuki (2), Kappei Tsukahara (1), Yasuto Yokoi (2), Junro Kuromitu (1), Ken Aoshima (2), Takeshi Nagasu (1)
(1) Tsukuba Research Laboratories, Eisai Co., Ltd, 5-1-3, Tokodai, Tsukuba, 300-2635, JAPAN; (2) Mitsui Knowledge Industry Co., Ltd., Harmony Tower 21St Floor 32-2, Honcho 1-Chome, Nakano-ku, Tokyo 164-8721 Japan

4-9

A compendium of genome-wide drug hypersensitivity screens provides insight into compound mode-of-action.
Ainslie B. Parsons (1), Andres Lopez (1), Renee Brost (1), David Williams (2), Raymond Anderson (2), Timothy Hughes (1), Charles Boone (1)
(1) Best Institute, University of Toronto, 112 College St, Toronto, On, M5G 1L6, Canada; (2) Department of Chemistry, University of British Columbia, Vancouver, Canada

4-10

Personal Robotics for Omics with High Density Yeast Arrays.
Charles Boone (1), Amy Tong (1), Pawel Kusan (2), Harry Singer (3), Carl Singer (3)
(1) Best Institute, University of Toronto, 112 College St, Toronto, ON, M5G 1l6, Canada; (2) Engineering Services Inc., Toronto Ontario Canada M5R 2J7; (3) Singer Instrument Co Ltd, Roadwater, Watchet, Somerset TA23 ORE England

4-11

Genetic screening of the yeast gene disruption library using an efficient plasmid transfer method.
Rodney Rothstein, Ivana Sunjevaric, Robert J.D. Reid
Genetics & Development, Columbia University Med. Ctr., 701 W 168th Street, New York, NY, 10032-2704, USA

Workshop & Poster Abstracts Topic 5 - Control of cell growth and proliferation

5-1

Regulation of APC/C by a protein complex containing the 14-3-3 homologs Bmh1 and Bmh2.
Mark Hall, Brian Billings, Mary Jeong, Juan Martinez, Anindya Chatterjee
Biochemistry Department, Purdue University, 175 S. University St, West Lafayette, IN, 47907, United States

5-2

Mitochondrial permeability transition in yeast as a means to dissipate energy.
Victoriano Pérez-Vázquez, Andrés Rojas-Hernández, Sergio Guerrero-Castillo, Salvador Uribe
Inst de Fisiología Celular, Univ Nal Autónoma de México, Ciudad Universitaria, México, DF, 04510, Mexico

5-3

Two RNA binding proteins Msa1 and Msa2 inhibitory to sexual differentiation in fission yeast.
Hee Tae Jeong, Yasuo Oowatari, Katsunori Tanaka, Hideyuki Matsuda, Makoto Kawamukai
Life & Environmental Science, Shimane University, 1060 Nishikwatsu, Matsue, 690-8504, Japan

5-4

The TOR and EGO protein complexes control microautophagy in yeast.
Frédérique Dubouloz, Valeria Wanke, Elisabetta Cameroni, Olivier Deloche, Claudio De Virgilio
Dep. Microbiol. and Mol. Med., University of Geneva, CMU, 1 rue Michel-Servet, Geneva 4, CH-1211, Switzerland

5-5

A role for Ppz1p-mediated regulation of the Trk1p high affinity potassium transporter in cell cycle progression.
Stephanie Merchan, Ramon Serrano, Lynne Yenush
IBMCP, Uni. Politecnica de Valencia, Camino de vera, Valencia, 46022, Spain

5-6

Bridge-induced translocation (BIT) by targeted DNA integration in yeast.
Valentina Tosato, Carlo V. Bruschi
Microbiology Laboratory, ICGEB, AREA, Padriciano 99, Trieste, I-34012, Italy

5-7

Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant.
Cleslei F. Zanelli, Sandro R. Valentini
Biological Sciences, School of Pharmacy - UNESP, Rod. Araraquara-Jau, Araraquara, SP, 14801-902, Brazil

5-8

Ccr4-Not complex mRNA deadenylase activity contributes to DNA damage responses in Saccharomyces cerevisiae.
Ana Traven, Andrew Hammet, Nora Tenis, Jorg Heierhorst
Molecular Genetics, St.Vincents's Institute, 9 Princes Street, Melbourne, 3065, Australia

5-9

Role of Sic1 as component of the cell sizer mechanism involved in the nutrient dependent regulation of the G1 to S transition in Saccharomyces cerevisiae.
Vittoria Zinzalla, Riccardo L. Rossi, Andrea Mastriani, Marco Vanoni, Lilia Alberghina
Dip.Biotecnologie e Bioscienze, Università di Milano-Bicocca, P.za della Scienza 2, Milano, 20126, Italy

5-10

A role for mRNA processing proteins Kem1 and Pat1 in spindle positioning during mitosis.
Christian Leisner, Mahamadou Faty, Yves Barral
Institute of Biochemistry, ETH Hoenggerberg, Schafmattstrasse 18, Zurich, 8093, Switzerland

5-11

The universal solvent DMSO (dimethyl sulfoxide) affects genome stability in S. cerevisiae.
Anna Chelstowska, Joanna Rytka
Dept. of Genetics, Inst.Biochemistry Biophysics, Pawinskiego 5A, Warsaw, 02-106, Poland

5-12

Periodic gene expression program of the fission yeast cell cycle.
Gabriella Rustici (1), Juan Mata (1), Katja Kivinen (2), Pietro Lio (2), Chis J. Penkett (1), Gavin Burns (1), Jacqueline Hayles (3), Alvis Brazma (2), Paul Nurse (4), Jurg Bahler (1)
(1) The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, U.K.; (2) EMBL Outstation-Hinxton, European Bioinformatics Institute, Cambridge CB10 1SD, U.K.; (3) Cancer Research UK London Research Institute, London WC2A 3PX, U.K.; (4) The Rockefeller University, New York 10021, USA

5-13

Regulation of Cdc14 phosphatase activity by Separase.
Ethel Queralt-Badia, Chris Lehane, Frank Uhlmann
chromosome segregation lab, Cancer Research, 44, LIF, London, WC2A 3PX, United Kingdom

5-14

The ribosome and the secretory apparatus: allies in cell growth and division.
Elisabeth Hoflehner (1), Thomas Karl (1), Karin Pachler (1), Burghard Scheibe (2), Michaela Eder (1), Eva Mitterhumer (1), Alena Pichova (3), Friedrich Lottspeich (4), Monika Zobawa (4), Elisabeth Hochleitner (4), Michael Breitenbach (1), Lore Koller (1)
(1) Dept. of Cell Biology, University of Salzburg, Hellbrunnerstr. 34, Salzburg, 5020, Austria; (2) Amersham Biosiences, D-79106 Freiburg, Germany; (3) Institute of Microbiology, Czech Academy of Science, Prague, Czech Republic; (4) MPI of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany

5-15

Ribosomal proteins of the ribosome subunit interface: does topology determine function?
Eva Mitterhumer (1), Karin Pachler (1), Kamil Önder (2), Thomas Karl (1), Albert Thür (1), Michael Löffler (3), Elisabeth Hoflehner (1), Peter Eckl (1), Klaus Richter (1), Michael Breitenbach (1), Lore Koller (1)
(1) Dept. of Cell Biology, University of Salzburg, Hellbrunnerstr. 34, Salzburg, 5020, Austria; (2) LKH, Dept. of Dermatology, Müllner Hauptstraße 48, A-5020 Salzburg; (3) AKH/I.M.III Währinger Gürtel 18-20, A-1090 Vienna, Austria

5-16

Screening for new components of the TOR-pathway in yeast.
Johan Kriel, Ole Lagatie, Matthias Versele, Johan Thevelein
Dep. of Molecular Microbiology, Katholieke Universiteit Leuven, Kasteelpark Arenb 31, Leuven-Heverlee, B-3001, Belgium

5-17

Replication Fork Block protein Fob1 co-operates with Cdc14 in resolving yeast chromosome XII distal regions.
Felix Machin, Jordi Torres-Rosell, Luis Aragon
Clinical Science Centre, Medical Research Council, Du Cane Road, London, W12 0NN, United Kindom

5-18

Growth control of the eukaryote cell: A systems biology study in yeast.
Juan I. Castrillo (1), Leo A. Zeef (1), David C. Hoyle (2), Nianshu Zhang (1), Andrew Hayes (1), David C. J. Gardner (1), Michael Cornell (3), June Petty (1), Marie Brown (4), Leanne Wardleworth (1), Sarah R. Hart (1), Simon J. Gaskell (4), Norman W. Paton (3), Kathryn S. Lilley (5), Douglas B. Kell (4), Stephen G. Oliver (1)
(1) FLS. Michael Smith Building, The University of Manchester, Oxford Road, Manchester, M13 9PT, UK; (2) School of Engineering, Computer Science and Mathematics, Harrison Building, University of Exeter, North Park Rd., Exeter, EX4 4QF, UK.; (3) School of Computer Science, Kilburn Building. The University of Manchester. Oxford Road. Manchester M13 9PL, UK.; (4) School of Chemistry, Faculty of Engineering and Physical Sciences, The University of Manchester. PO Box 88, Manchester M60 1QD, UK.; (5) Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Building O, Downing Site, Cambridge, CB2 1QW, UK.

5-19

A putative lipid-binding protein and two cyclin-dependent kinases act in a pathway coordinating nuclear divisions and gametogenesis in S. cerevisiae.
Gela G. Tevzadze, Jessica V. Pierce, Rochelle E. Esposito
Mol Genetics and Cell Biology, The University of Chicago, 920 East 58th Str, Chicago, IL, 60637, USA

5-20

Involvement of Slk19 in Chromosome Segregation and Mitotic Exit.
Tao Zhang, Hong Hwa Lim, Chee Seng Cheng, Uttam Surana
Cell Cycle Control, IMCB, 61 Biopolis Drive, Singapore, 138673, Singapore

5-21

Proteomic and transcriptomic analysis of FAR1 mutants in Saccharomyces cerevisiae.
Lorenzo Querin (1), Rossella Sanvito (2), Valeria Mapelli (1), Fulvio Magni (2), Alain Van Dorsselaer (3), Marzia Galli Kienle (2), Lilia Alberghina (1), Marco Vanoni (1)
(1) Biotecnologie e Bioscienze, University of Milano Bicocca, Piazza della Scienza, Milano, 20126, Italy; (2) University of Milano Bicocca, DIMESAB, Monza, Italy; (3) CNRS, University Louis Pasteur, LSMBO, Strasbourg, France

5-22

The cyclin dependent kinase Pho85 regulates G1 progression through the transcriptional repressor Whi5.
Jonathan Millman, Dongqing Haung, Richelle Sopko, Brenda Andrews
Molecular and Medical Genetics, University of Toronto, 1 King's College, Toronto, ON, L9H 1M9, Canada

5-23

The role of the Anaphase Promoting Complex in the cell cycle of the filamentous fungus A. gossypii.
Nicoleta Sustreanu, Peter Philippsen, Amy S. Gladfelter
Applied Microbiology, Biozentrum, Basel University, Kingelbergstrasse 50, Basel, CH-4056, Switzerland

5-24

Novel genes required for meiotic chromosome segregation identified in a high-throughput knock-out screen.
Juraj Gregan, Kirsten Rabitsch, Kim Nasmyth
IMP, IMP, Dr. Bohr-Gasse 7, Vienna, A-1030, Austria

5-25

(How) Does DNA enter the cohesin ring?
Stephan Gruber, Kim Nasmyth
IMP, IMP, Dr. Bohr-Gasse 7, Vienna, 1030, Austria

5-26

Mnd2 is an Antagonist of the Meiosis Specific APC/C-Ama1, Essential to Sustain normal Chromosome Structure during Meiotic Prophase.
Alexandra M. Penkner, Martin Xaver, Franz Klein
Vienna Biocenter II, Max Perutz Laboratories, Department of Chromosome Biology, Dr. Bohr-Gasse 1, A-1030 Vienna, Austria

Workshop & Poster Abstracts Topic 6 - DNA replication, recombination and repair

6-1

The mating-type-switch activating protein Sap1 is also required for replication fork blockage at the ribosomal DNA in Schizosaccharomyces pombe.
Eva Mejía-Ramírez, Alicia Sánchez-Gorostiaga, Dora B. Krimer, Jorge B. Schvarzman, Pablo Hernández
Biología Celular y del Desarr., CIB, CSIC, Ramiro de Maeztu, 9, Madrid, 28040, Spain

6-2

The mechanism of homology search during double-strand break repair.
Martin Kupiec, Batia Liefshitz, Shira Goldstein, Adi Barzel
Mol. Micro & Biotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel

6-3

Role of ELG1 in genome stability, telomere length regulation and telomeric silencing.
Shay Ben-Aroya, Sarit Smolikov, Yuval Mazor, Oren Parnas, Martin Kupiec
Mol. Micro & Biotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel

6-4

Variation in telomere length control, senescence and genetic instability in Saccharomyces yeasts.
Gianni Liti, Svasti Haricharan, Nicole Guzman, Parvez Lokhandwala, Edward Louis
Genetics, University of Leicester, University Road, Leicester, LE17RH, UK

6-5

Genomic instability, DNA damage and cell cycle arrest associated with rad54 rdh54 diploids.
Atul Kulkarni (1), Giordano Liberi (2), Hannah Klein (1)
(1) Biochemistry, NYU Medical Center, 550 First Avenue, New York, NY, 10016, USA; (2) FIRC Institute of Molecular Oncology, Via Adamello 16, 20141 Milan, Italy

6-6

Molecular analysis of transcription-associated recombination (TAR) and mutation (TAM).
Belen Gomez, Andres Aguilera
Genetics, University of Seville, Reina Mercedes,6, Seville, 41012, Spain

6-7

Two Rad51-dependent recombination repair pathways in fission yeast.
Yufuko Akamatsu, Nami Haruta, Yumiko Kurokawa, Hiroshi Iwasaki
Super-Molecular Biology, Yokohama City University, Suehiro, Tsurumi, Yokohama, 230-0045, Japan

6-8

The role of rrp1 gene in the repair of DNA damage in Schizosaccharomyces pombe.
Dorota Dziadkowiec (1), Pawel Karpinski (1), Hiroshi Iwasaki (2)
(1) Inst. Genetics & Microbiology, Wroclaw University, Przybyszewskiego 63, Wroclaw, 51-148, Poland; (2) Grad. Sch. of Integrated Sci., Yokohama City University, 1-7-29, Suehiro-cho, Tsurumi, Yokohama, 230-0045, Japan

6-9

Chromosomal dynamics in Saccharomyces cerevisiae : influence of genetic context on the types of obtained rearrangements.
Emilie Fritsch, Jean Luc Souciet, Serge Potier, Jacky de Montigny
Microbiologie et Génétique, Institut de Botanique, 28 rue Goethe, Strasbourg, 67000, France

6-10

Increased frequency of site-specific DSBs by addition of nuclear localization signal to the yeast mitochondrial I-SceI endonuclease.
Sandra Gregoric (1), Mladen Mercep (1), Carlo V. Bruschi (2), Kresimir Gjuracic (1)
(1) Biology, PLIVA Research Institute Ltd., Prilaz b. Filipovica, Zagreb, 10000, Croatia; (2) ICGEB, Area Science Park, Padriciano 99, I-34012 Trieste, Italy

6-11

Isolation of new TEL1 separation-of-function alleles.
David Faucher, Raymund J. Wellinger
Microbiology, Sherbrooke University, 3001 12th avenue, Sherbrooke, QC, J1H 5N4, Canada

6-12

Studies on the structure of telomerase RNA.
Nancy Levesque, Alain T. Dandjinou, Raymund J. Wellinger
Microbiology, Universite de Sherbrooke, 3001 12th avenue, Sherbrooke, Qc, J1H 5N4, Canada

6-13

DNA duplex destabilization: A highly conserved property of regulatory regions of yeast genome.
Prashanth Ak, Craig Benham
Genome Center, University of California, Davis, 1 Shileds Avenue, Davis, CA, 95616, USA

6-14

Molecular genetic analysis of some artificial interspecific Saccharomyces cerevisiae x S. uvarum hybrids.
Zsuzsa Antunovics, Matyas Sipiczki
Genetics and Molecular Biology, University of Debrecen, Egyetem 1, Debrecen, 4010, Hungary

6-15

Dual role of MSH1 in maintaining the stabilty of mitochondrial DNA of Saccharomyces cerevisiae.
Marta Fikus-Krynska, Piotr Koprowski, Piotr Dzierzbicki, Zygmunt Ciesla
Laboratory of DNA Repair, IBB PAS, Pawinskiego 5a, Warsaw, 02-106, Poland

6-16

The genotoxic effects of yIme2 and hCDK2 in S. cerevisiae cell cycle.
Moran Szwarcwort Cohen, Zohar Kasulin Boneh, Yona Kassir
Biology, Technion, Technion city, Haifa, 32000, Israel

6-17

Localization of Spo11 and Mre11 recombinase on yeast chromosomes during meiosis.
Kazuto Kugo (1), Hiroyuki Sasanuma (1), Saori Mori (2), Yuki Katou (2), Katsuhiko Shirahige (2), Takehiko Shibata (3), Kunihiro Ohta (1)
(1) Genetic Dynamics Research Unit, RIKEN, 2-1 Hirosawa, Wako, 351-0198, Japan; (2) Center for Biological Resources and Informatics, Division of Gene Research, and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan; (3) Shibata Distinguished Senior Scientist Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

6-18

How are repeated gene clusters maintained?
Takehiko Kobayashi, Austen Ganley
Division of Genome Dynamics, Natl. Inst. for Basic Biol., Myodaijicho, Okazaki, 444-8585, Japan

6-19

Study of the influence of mutations in regulatory genes of the PHO system on mutation rates of the cells of yeast Saccharomyces cerevisiae.
Arseni Smirnov (1), Anastasia Fizikova (2), Elena Sambuk (2)
(1) 188309 Russia, Leningradskaya region, Gatchina, Slepneva st., 4-2-9; (2) Genetics and Selection, St.Petersburg state university, Universitetskaya, St. Petersburg, 199034, Russian Federation

6-20

Analyses of functional elements and their conservation between Saccharomyces and Kluyveromyces telomerase RNAs.
Majdi Kabaha, Irit Schwartz, Yogev Brown, Sivan Pearl, Mira Abraham, Yehuda Tzfati
Department of Genetics, Hebrew University of Jerusalem, Givat Ram, Jerusalem, 91904, Israel

6-21

The SQ/TQ-cluster domain protein Mdt1 has lesion-specific DNA damage response functions in Saccharomyces cerevisiae.
Brietta L. Pike, Nora Tenis, Jörg Heierhorst
Molecular Genetics Unit, St. Vincent's Institute, 9 Princes Street, Melbourne-Fitzroy, 3065, Australia

6-22

Identification of a small molecule that activates DNA replication checkpoint in yeast and ATM/ATR pathway in mammalian cells.
Vaidehi Krishnan, Hong Hwa Lim, San Ling Si-Hoe, Uttam Surana
Cell Cycle Control, IMCB, 61 Biopolis Drive, Singapore, 138673, Singapore

6-23

In vitro characterisation of Saccharomyces castellii Cdc13.
Jenny Rhodin, Marita Cohn
Cell- and Organism Biology, Lund University, Sölvegatan 35, Lund, S-223 62, Sweden

6-24

Essential role of the Smc5/6 complex in segregation of the rDNA locus.
Jordi Torres-Rosell, Félix Machín, Sarah Farmer, Stefan Uhle, Giacomo de Piccoli, Luis Aragón
Cell Cycle Group, MRC Clinical Sciences Centre, Du Cane Road, London, W12 0NN, UK

6-25

Roles of sgs1 and srs2 helicases in cag/ctg repeat intability in Sacharomyces cerevisiae.
Alix Kerrest, Bernard Dujon, Guy-Franck Richard
Génétique moléculaire, Institut Pasteur, rue du docteur Roux, Paris, 75015, France

6-26

Rercuitment of the budding yeast Rad9 protein to sites of DNA damage.
Aisling O'Shaughnessy (1), Geraldine Toh (2), Ian Dobbie (2), Stefano Maffini (2), Muriel Grenon (2), Anne O'Rorke (2), Noel Lowndes (2)
(1) Genome Stability LaboratoryDepartment of Biochemistry, National University of Ireland, Galway.; (2) Biochemistry, NUI Galway, Ireland, University Road, Galway, N/A, Ireland

6-27

A mutation in Dbf4 motif M impairs interactions with DNA replication factors and confers increased resistance to genotoxic agents.
Angela A. Varrin, Ajai A. Prasad, Rolf-Peter Scholz, Matthew D. Ramer, Bernard P. Duncker
Biology, University of Waterloo, 200 University Ave., Waterloo, ON, N2L 3G1, Canada

6-28

A conserved triple-helical stem in a yeast telomerase RNA pseudoknot is essential for telomerase function.
Kinneret Shefer (1), Valentin Gorkovoy (1), Yogev Brown (1), Mira Abraham (1), Sivan Pearl (1), Nikolai Ulyanov (2), Yehuda Tzfati (1)
(1) Department of Genetics, Hebrew University of Jerusalem, Givat Ram, Jerusalem, 91904, Israel; (2) Department of Parmaceutical Chemistry, University of California, San Francisco, CA 94143-2280, USA

6-29

Systematic identification and characterization of genes that suppress gross chromosomal rearrangements.
Pamela Kanellis, Aimee Galacia, Melanie Partington, Daniel Durocher
Molecular and Medical Genetics, Samuel Lunenfeld/U of Toronto, 600 University Ave, Toronto, ON, M5G1X5, Canada

6-30

The mRNA deadenylase Ccr4 contributes to the tolerance of replication stress through a pathway that involves the DNA damage transcriptional repressor Crt1.
Robert N. Woolstencroft, Michael A. Cook, Mike Tyers, Daniel Durocher
Samuel Lunenfeld Research Inst, Mount Sinai Hospital, 600 University Ave, Toronto, ON, M5G 1X5, Canada

6-31

Further characterization of the Saccharomyces cerevisiae Pso2 protein.
Zuzana Dudásová (1), Andrej Dudás (1), Danusa Vlasáková (1), Dana Vigasová (2), Daniela Gabč ová (3), Miroslav Chovanec (1)
(1) Lab. of Molecular Genetics, Cancer Research Institute, Vlárska 7, Bratislava, 833 91, Slovak Republic; (2) Faculty of Natural Sciences, Department of Biology, University of Matthiae Belii, Banská Bystrica, Slovak Republic; (3) Faculty of Natural Sciences, Department of Genetics, Comenius University, 842 15 Bratislava, Slovak Republic

6-32

Effect of sodium selenite on induction of DNA damage in Saccharomyces cerevisiae.
Lucia Letavayová (1), Viera Vlč ková (2), Danusa Vlasáková (1), Eva Marková (1), Miroslav Chovanec (1), Jela Brozmanová (1)
(1) Laboratory of Molecular Genetics, Cancer Research Institute, Vlárska 7, 833 91 Bratislava, Slovak Republic; (2) Department of Genetics, Faculty of Natural Sciences, Mlynská dolina B-1, Bratislava, 842 15, Slovak Republic

6-33

The Saccharomyces cerevesiae RAD9 gene is required, mainly, for repair of the transcribed strand through up-regulation of DNA repair genes.
Nisreen Al-Moghrabi, Ibtehaj Al-Sharif, Abdelilah Aboussekhra
Biological & medical research, Kingfaisl specialist hospital, Takkassosy road, Riyadh, 11211, Kingdom of Saudi Arabia

6-34

Repair of DNA interstrand cross-links in G1 phase Saccharomyces cerevisiae cells requires DNA Polymerase Zeta.
Sovan Sarkar, Peter McHugh
Cancer Research UK Labs, WIMM, Oxford University, J.R Hospital, Oxford, OX3 9DS, UK

6-35

New insight into mediator function of yeast Rad52 protein.
Lumir Krejci (1), Iben Plate (2), Binwei Song (3), Michael Sehorn (3), Wendy Bussen (3), Uffe H. Mortensen (2), Patrick Sung (3)
(1) Masaryk University, Kotlarska 2, Brno 60200, Czech Republic and Yale University, 333 Cedar St, SHM C130A, New Haven, CT 06511, USA; (2) BioCentrum-DTU, Soeltofts Plads Bldg 223, DK-2800 Lyngby, Denmark; (3) Yale University, 333 Cedar St, SHM C130A, New Haven, CT 06511, USA

6-36

Control of ploidy occurs at the individual chromosome level in the yeast S. cerevisiae.
Carlo V. Bruschi, Sanjeev K. Waghmare
Microbiology, ICGEB, AREA, Padriciano 99, Trieste, I-34012, Italy

6-37

Srs2 and Sgs1 DNA helicases associate with Mre11 in different sub-complexes following checkpoint activation and CDK1-mediated Srs2 phosphorylation.

Irene Chiolo (1), Walter Carotenuto (1), Giulio Maffioletti (2), John H.J. Petrini (3), Marco Foiani (1), Giordano Liberi (1)
(1) FIRC Institute of Molecular On, IFOM, Via Adamello, 16, Milano, 20139, Italy; (2) The University of Dundee, Dow Street, Dundee, DD1 5EH, UK; (3) Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY, 10021, USA

Workshop & Poster Abstracts Topic 7 - Cell signaling and stress responses

7-1

Characterization of S. cerevisiae Hog1 protein activation upon bacterial LPS-exposure.
Joana M. Marques (1), Ricardo J. Rodrigues (2), Teresa Gonçalves (1)
(1) CNC, Inst.Microbiology, FMUC, Rua Larga, Coimbra, 3004-504, Portugal; (2) CNC, Inst. Biochemistry, FMUC, Rua Larga, Coimbra, 3004-504, Portugal

7-2

The regulatory role of Mss11p in Ca2+-dependent flocculation and haploid invasive growth in Saccharomyces cerevisiae.
Michael C. Bester (1), Ricardo R. Cordero Otero (1), Isak S. Pretorius (2) and Florian F. Bauer (1)
(1) Institute for Wine Biotechnology, Stellenbosch University, Stellenbosch, ZA-7600, South Africa; (2) Australian Wine Research Institute, Urrbrae Glen Osmond, Adelaide, SA 5064, Australia

7-3

Improving genetic tools to direct gene cloning in the osmoresistant yeast Zygosaccharomyces rouxii.
Lenka Pribylova (1), Jacky de Montigny (2), Serge Potier (2), Hana Sychrova (1)
(1) Dept. Membrane Transport, Inst. Physiology CzAcadSci, Videnska 1083, Prague, 14220, Czech Republic; (2) Lab. Microbiologie et Genetique, Inst. Botanique ULP-CNRS, 8 rue Goethe, 67083 Strasbourg, France

7-4

A proteome analysis of the yeast response to the herbicide 2,4-dichlorophenoxyacetic acid.
Miguel C. Teixeira (1), Pedro M. Santos (1), Alexandra R. Fernandes (2), Isabel Sá-Correia (1)
(1) Biological Sciences Research Group, CEBQ, Instituto Superior Tecnico, 1049-001 Lisbon, Portugal; (2) Biological Sciences Research Group, CEBQ, Instituto Superior Tecnico, 1049-001 Lisbon, Portugal; Universidade Lusófona de Humanidades e Tecnologias, Campo Grande, 376, 1749-029 Lisbon, Portugal

7-5

The adaptive response of Saccharomyces cerevisiae to weak acids involves the Haa1p-regulon.
Alexandra R. Fernandes (1), Rita C. Vargas (2), Nuno P. Mira (2), Inês Canelhas (2), Isabel Sá-Correia (2)
(1) Biological Sciences Research Group, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001 Lisboa, Portugal., Universidade Lusófona de Humanidades e Tecnologias, Campo Grande,376, 1749-029 Lisboa, Portugal; (2) Biological Sciences Research Group, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001 Lisboa, Portugal.

7-6

New mechanisms regulating gene PHO3 and PHO5 expression in yeast Saccharomyces cerevisiae depending on medium composition.
Vladimir A. Savinov (1), Anastasia Y. Fizikova (2), Elena V. Sambuk (3)
(1) St.-Petersburg, 192283, Budapeshtskaya st., 91-1, 129, Russia; (2) St.-Petersburg, 197373, pr. Aviakonstructorov, 44-2, 120, Russia; (3) St.-Petersburg, 195112, Novocherkassky pr., 26, 117, Russia

7-7

Aquaporin expression and freeze tolerance in yeast.
An Tanghe, Johan Thevelein, Patrick Van Dijck
Labo Moleculaire Celbiologie, Instit. Plantk. en Microbiol., K. park Arenberg 31, Heverlee, 3001, Belgium

7-8

Characterisation of Cdc42p-mutants affecting pheromone response and invasive growth in S. cerevisiae.
Melanie Bolte, Tim Koehler, Diana Kruhl, Hans-Ulrich Moesch
Genetics, Philipps University Marburg, Karl-von-Frisch-Str, Marburg, 35032, Germany

7-9

Regulation of the TEA/ATTS transcription factor Tec1 by the MAP kinases Fus3 and Kss1.
Stefan Brückner (1), Tim Köhler (2), Barbara Heise (1), Hans-Ulrich Mösch (1)
(1) Genetics, Philipps University Marburg, Karl von Frisch Str., Marburg, 35032, Germany; (2) Degussa AG, Rodenbacher Chaussee 4, 63457 Hanau, Germany

7-10

Molecular basis of cold perception: a rigidification of the plasma membrane induces transcription via the HOG MAP Kinase pathway in Saccharomyces cerevisiae.
Joaquin Panadero, Claudia Pallotti, Sonia Rodriguez-Vargas, Francisca Randez-Gil, Jose Antonio Prieto
Department of Biotechnology, Instituto de Agroquimica y Tecnologia de los Alimentos (CSIC), P.O.Box 73, E-46100-Burjassot, Valencia, 46100, Spain

7-11

Genetic regulation mediated by thiamin pyrophosphate-binding motif.
Kazuto Nosaka (1), Mari Onozuka (2), Yuko Kawasaki (3), Hiroyuki Konno (1), Kenichi Akaji (1)
(1) Chemistry, Kyoto Pref. Univ. Med., Kita-ku, Kyoto, 603-8334, Japan; (2) Biochemistry and Molecular Biology, Kyoto Pref. Univ. Med., Kamigyo-ku, Kyoto 602-8566, Japan; (3) Doshisha Women's College, Kamigyo-ku, Kyoto 602-0893, Japan

7-12

The cyclin-dependent protein kinase Pho85 regulates Rim15 function.
Valeria Wanke, Elisabetta Cameroni, Ivo Pedruzzi, Frédérique Dubouloz, Claudio De Virgilio
Dep. Microbiol. and Mol. Med., University of Geneva, CMU, 1 rue Michel-Servet, Geneva 4, CH-1211, Switzerland

7-13

Tor and the cAMP-PKA signaling convey separate inputs to ribosomal protein gene promoters.
Sara A. Zurita-Martinez, Maria E. Cardenas
Mol. Genetics and Microbiology, Duke University Medical Center, Research Drive, Durham, NC, 27707, USA

7-14

Adaptive Al-resistance in Rhodotorula glutinis IFO1125.
Akio Tani, Takaya Kawahara, Yoko Yamamoto, Kazuhide Kimbara, Fusako Kawai
Res. Inst. Biores., Okayama Univ., Chuo 2-20-1, Okayama, 710-0046, Japan

7-15

Transcriptional profiling in the short and long term response to nitrogen availability: direct and indirect targets of both activators Gln3 and Gat1.
Bart Scherens, André Feller, Fabienne Vierendeels, Francine Messenguy, Evelyne Dubois
Microbiology, IRMW-ULB, Av. E. Gryzon,1, Brussels, 1070, Belgium

7-16

Cph1 negatively regulating MDR1 involved in drug resistance in Candida albicans.
Yun-Liang Yang (1), Yeong-Yi Kao (1), Chia-Geun Chen (2), Chia-Li Su (2), Ming-Yang Tsao (2), Hsiu-Jung Lo (2)
(1) Dept Biol. Sci. & Tech., National Chiao Tung University, 75 Pao-Ai Street, Hsin-Chu, 300, Taiwan, ROC; (2) Division of Clinical Research, National Health Research Institutes, Miaoli, Taiwan, R.O.C.

7-17

Metabolic engineering of threonine / methionine biosynthetic pathway and its effect in stress response in S. cerevisiae.
M.J.R. Palero, Isabel L. Calderon
Department of Genetics, University of Seville, Avd. Reina Mercedes, Seville, 41012, Spain

7-18

The role of CDC25 in the control of cell cycle and the activity of two stress transcriptional factors: Skn7 and Hsf1.
Claudia Martinez-Anaya, Sergio Perez-Landero, Luz Maria Martinez, Jorge Nieto-Sotelo
Plant Molecular Biology, Inst. de Biotecnologia, UNAM, Av. Universidad 2001, Cuernavaca, 62210, Mexico

7-19

Effect of pH and NaCl on the plasma membrane fluidity of the marine yeast Debaryomyces hansenii.
Vera Montiel (1), Martina Turk (2), Ana Plemenitas (2), Jose Ramos (1)
(1) Microbiology, University of Cordoba, Campus de Rabanales, Cordoba, Es, E14080, Spain; (2) Institute of Biochemistry, Medical Faculty, University of Ljubljana, Slovenia

7-20

Involvement of Yap1p in calcineurin-mediated G₂ cell-cycle regulation in Saccharomyces cerevisiae.
Hiroshi Yokoyama, Masaki Mizunuma, Michiyo Okamoto, Josuke Yamamoto, Dai Hirata, Tokichi Miyakawa
Molecular Biotechnology, AdSM, Hiroshima University, 1-3-1, Higashi-Hiroshima, 739-8530, Japan

7-21

Saccharomyces cerevisiae as a model to explore inter-kingdom communication between bacteria and fungi.
Olive Gleeson (1), Fergal O'Gara (2), John Morrissey (3)
(1) Department of Microbiology, University College Cork, Cork. Ireland; (2) Biomerit Research Centre, Department of Microbiology, University College Cork, Cork. Ireland; (3) Department of Microbiology, University College Cork, College Road, Cork, Ireland

7-22

Early yeast response to stress induced by the antimalarial drug artesunate involves the Pdr1p/Pdr3p-mediated transcriptional activation of TPO1 and PDR5 genes.
Marta Alenquer, Sandra Tenreiro, Isabel Sá-Correia
Biolog. Scienc. Research Group, Instituto Superior Técnico, Av. Rovisco Pais, Lisbon, 1049-001, Portugal

7-23

The Hal3 family in Saccharomyces cerevisiae : novel phosphatase-unrelated functions.
Amparo Ruiz, Ivan Muñoz, Raquel Serrano, Asier González, Joaquin Ariño
Bioquimica Biologia Molecular, Universitat Autonoma Barcelona, Campus UAB, Bellaterra, 08193, Spain

7-24

Effects of tea tree oil on cell integrity signalling in yeast.
Andrea Straede, Jürgen J. Heinisch
Department of Genetics, University of Osnabrück, Barbarastr.11, Osnabrück, 49069, Germany

7-25

Protein kinase C and its interactions in Kluyveromyces lactis.
Sabrina Koch, Hans-Peter Schmitz, Rosaura Rodicio, Jürgen J. Heinisch
Department of Genetics, University of Osnabrück, Babarastrasse 11, Osnabrück, 49069, Germany

7-26

The role of Sod1 and Sod2 proteins in the protection of Saccharomyces cerevisiae cells against multiple stresses.
Dorota Dziadkowiec (1), Anna Krasowska (1), Anna Liebner (1), Karel Sigler (2)
(1) Inst. Genetics & Microbiology, Wroclaw University, Przybyszewskiego 63, Wroclaw, 51-148, Poland; (2) Institute of Microbiology, Academy of Science of the Czech Republic, 142 20 Prague 4, Czech Republic

7-27

Rgd1, the RhoGAP of Rho3 and Rho4, is a phosphorylated protein that interacts with various phospholipids.
Helder Fernandes, Olivier Roumanie, Xavier Gatti, Sandra Claret, Didier Thoraval, Marc Crouzet, François Doignon
LMBS, IBGC UMR-CNRS 5095, 146,Rue Léo Saignat, Bordeaux, 33076, France

7-28

Arsenic-activation of the yeast AP-1-like transcription factors Yap1 and Yap8.
Yujun Di, Markus J. Tamás
CMB/Microbiology, Göteborg University, Box 462, Göteborg, 40530, Sweden

7-29

Low-pH response in Saccharomyces cerevisiae : implication of the RhoGAP Rgd1p and the PKC pathway.
Xavier Gatti, Sandra Claret, Helder Fernandes, Olivier Roumanie, François Doignon, Marc Crouzet, Didier Thoraval
LBMS, IBGC UMR CNRS/U Bordeaux2 5095, 146 rue léo saignat, Bordeaux, 33076, France

7-30

The cellular response to arsenic exposure.
Markus J. Tamás (1), Michael Thorsen (1), Jean Labarre (2)
(1) CMB - Microbiology, Göteborg University, Box 462, Göteborg, 405 30, Sweden; (2) Service de Biochimie et de Génétique Moléculaire, DBJC, CEA/Saclay, 91191 Gif-sur-Yvette, France

7-31

Retrophosphorylation of Mkk1 and Mkk2 by the MAP kinase Slt2: Fine-tuning of the cell integrity pathway?
Maria Jimenez, Victor J. Cid, Maria Molina
Microbiologia II, Universidad Complutense, Plaza Ramon y Cajal, Madrid, 28040, Spain

7-32

Multiple signaling pathways triggered by alkaline stress in S. cerevisiae.
Raquel Serrano (1), Dolores Bernal (1), Antonio Casamayor (1), Humberto Martin (2), Joaquin Ariño (1)
(1) Bioquimica Biologia Molecular, Universitat Autonoma Barcelona, Edifici V. CampusUAB, Bellaterra, 08193, Spain; (2) Dept. Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza de Ramon y Cajal s/n, E-28040 Madrid, Spain

7-33

Salt tolerance in halophilic black yeast Hortaea werneckii may be influenced by putative ENA-ATPases.
Alenka Gorjan, Ana Plemenitas
University of Ljubljana, Faculty of Medicine, Institute of Biochemistry, Ljubljana, Slovenia

7-34

The minimum domain of Pho81 is not sufficient to control the Pho85-Rim15 effector branch involved in phosphate starvation-induced stress responses.
Erwin Swinnen, Joris Winderickx
Biology, KUL, Kasteelpark Arenberg, Heverlee, 3001, Belgium

7-35

Yap4 and Yap6 co-operate in mediating the yeast environmental stress response.
Tracy Nevitt, Jorge Pereira, Claudina Rodrigues-Pousada
Genomics and Stress Lab, ITQB, Av Republica, Oeiras, 2980, Portugal

7-36

Arrestin-related proteins mediate pH signaling in filamentous fungi and yeast.
Silvia Herranz (1), Jose M. Rodriguez (1), Henk-Jan Bussink (2), Joanna Rudnicka (2), Juan C. Sanchez (1), Herbert N. Arst (2), Miguel A. Peñalva (1), Olivier Vincent (1)
(1) Molecular Microbiology, C.I.B. - C.S.I.C., Ramiro de Maeztu, 9, Madrid, 28040, Spain; (2) Department of Infectious diseases, Faculty of medicine, Imperial college of Science, Techonology & Medecine, Du Cane Road, London W12 ONN, UK

7-37

A subunit of RNA polymerase II is involved in translation regulation through binding components of translation initiation complex.
Liat Harel, Lea Duek, Mordechai Choder
Molecular Microbiology, Technion, Rappaport building, Haifa, 31096, Israel

7-38

Sch9 and Rim15 control subsets of the rapamycin-induced transcriptional response in the yeast Saccharomyces cerevisiae.
Bart Smets, Johnny Roosen, Joris Winderickx
Biology, KU Leuven, Kasteelpark Arenberg, 31, Heverlee, 3001, Belgium

7-39

Characterization of the transcriptional response to Mg²⁺ starvation in Saccharomyces cerevisiae.
Gerlinde Wiesenberger, Jochen Stadler, Rudolf Schweyen
MFPL, Department of Genetics, University of Vienna, Dr. Bohrgasse 9, Vienna, 1030, Austria

7-40

The role of the yeast thioredoxin system in protection against reductive stress induced-protein aggregation.
Jonathan D. Rand, Chris M. Grant
Faculty of Life Sciences, The University of Manchester, Oxford Rd, Manchester, M13 9PT, United Kingdom

7-41

Hypoxia response in the pathogenic yeast Cryptococcus neoformans.
Vladislav Raclavsky (1), Jiri Pavlicek (1), Misako Ohkusu (2), Jitka Trtkova (1), Vendula Husickova (1), Radko Novotny (3), Jiri Kunert (1), Kanji Takeo (2), Susumu Kawamoto (2)
(1) Dept. of Biology, Palacky University, Hnevotinska 3, Olomouc, 775 15, Czech Republic; (2) Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba City, 260-8673, Japan; (3) Dept. of Microscopy Methods, Palacky University, Hnevotinska 3, Olomouc, 775 15, Czech Republic

7-42

Decoding physiological and regulatory mechanisms in the oxidative stress response by high resolution phenomics.
Jonas Warringer, Elke Ericson, Luciano Fernandez, Anders Blomberg
Microbiology, Gothenburg University, Medicinaregatan 9c, 41390, 1390, Sweden

7-43

Downstream components responsible for the higher stress resistance of the Saccharomyces cerevisiae fil1 adenylate cyclase mutant.
Cristina Vianna, Barbara Leyman, Johan Thevelein, Patrick Van Dijck
Molecular Microbiology, VIB and K.U. Leuven, Kasteelpark Arenberg, Leuven, B-3001, Belgium

7-44

A Proteomic Approach Identifies Sixty Cytoplasmic Proteins With Constitutively Oxidized Cysteine Residues. Effects Of H2O2, And Thiol-Redox Control Deficiency.
Natacha Le Moan, Frédérique Tacnet, Sophie Le Maout, Michel B. Toledano
LSOC SBMS DBJC, CEA SACLAY, Bat 142, Gif-sur-Yvette, 91191, France

7-45

Changes in the Candida albicans proteome induced by growth on different carbon sources.
Zhikang Yin, David Stead, Laura Selway, Janet Walker, Philip Cash, Alistair Brown
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK

7-46

Desiccation stress response in Saccharomyces cerevisiae.
Sooraj Ratnakumar (1), Bharat Rash (2), Andrew Hayes (2), Stephen Oliver (2), Alan Tunnacliffe (1)
(1) Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK; (2) Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK

7-47

Identification of wheat cDNAs conferring deoxynivalenol resistance in yeast.
Doris Lucyshyn (1), Hanna Weindorfer (1), Marc Lemmens (2), Gerhard Adam (1)
(1) DAPP - IAGC, BOKU Vienna, Muthgasse 18, Vienna, A-1190, Austria; (2) IFA-Tulln, Institute for Agrobiotechnology, Department of Plant Production, Konrad Lorenz Strasse 20, A-3430 Tulln, Austria

7-48

Multiple regulations and complex nucleocytoplasmic oscillatory behavior of Msn2, the transcriptionnal activator of the general stress response in Saccharomyces cerevisiae.
Michel Jacquet (1), Cécilia Garmendia (1), Emmanuelle Boy-Marcotte (1), Hervé Garreau (1), Sylvie Lallet (2), Albert Goldbeter (3)
(1) IGM UMR CNRS 8621, Université Paris-Sud, bat 400, Orsay, 91405, France; (2) Institut Jacques Monod, UMR 7592 CNRS, Universités Paris 6&7, 2, Place Jussieu, 75251 PARIS Cedex 05, FRANCE; (3) Faculté des Sciences, Université Libre de Bruxelles, Campus Plaine, CP 231, B-1050 Brussels, Belgium

7-49

Control of yeast MAPK function by the dual specificity phosphatase Msg5.
Marta Flandez, Clara Bermejo, Eva Tapia, M. Jose Marin, Humberto Martin, Maria Molina
Microbiologia II, Universidad Complutense, Plaza Ramon y Cajal, Madrid, 28040, Spain

7-50

Yap4p phosphorylation under osmotic stress.
Jorge Pereira, Tracy Nevitt, Claudina Rodrigues-Pousada
Genomics and Stress Laboratory, ITQB, UNL, apartado 127, Oeiras, 2781-901, Portugal

7-51

The genome-wide kinetics of S. cerevisiae response to oxidative stress induced by cumene hydroperoxide.
Ana Martins, Wei Sha, Pedro Mendes, Vladimir Shulaev
VA Bioinformatics Institute, VA Tech, Washington Street, Blacksburg, VA, 24060, USA

7-52

Analysis of bacterial toxin-antitoxin expression in yeast.
Meredith Prysak (1), Mohan Liu (1), Masayori Inouye (2), Nancy Woychik (1)
(1) Mol. Gen., Micro. & Immunol., UMDNJ-Robert Wood Johnson MS, 675 Hoes Lane, Piscataway, NJ, 08854, USA; (2) Department of Biochemistry, UMDNJ-Robert Wood Johnson MS, 675 Hoes Lane, Piscataway, NJ 08854 USA

7-53

How to sense methylglyoxal in yeast: activation of stress-activated protein kinase pathway in Schizosaccharomyces pombe.
Yoshifumi Takatsume, Shingo Izawa, Yoshiharu Inoue
Graduate School of Agriculture, Kyoto University, Gokasho, uji, Kyoto, 611-0011, Japan

7-54

Development of cadmium-detectable whole-cell systems using the methylotrophic yeast Hansenula polymorpha.
Jeong-Nam Park, Min Jeong Sohn, Doo-Byoung Oh, Joo-Hyung Heo, Sang Ki Rhee, Hyun Ah Kang
Metabolic Engineering Lab., KRIBB, 52Eoeun-dong,Yuseong, Daejeon, 305-333, Korea

7-55

Cellular processes affecting S. cerevisiae tolerance to hypochlorous acid.
Gabriel Perrone, Stefan Kirsch, Ian Dawes
School Biotech. & Biomol. Sci., University of New South Wales, Anzac Parade, Sydney, 2052, Australia

7-56

Genetic interaction between protein phosphatases SIW14, PPZ1 and all of protein kinases in Saccharomyces cerevisiae.
Masataka Hirasaki, Masaya Horiguchi, Yoshinobu Kaneko, Satoshi Harashima
Dept. Biotechnology, Grad. Schl. Eng., Osaka Univ., Yamadaoka 2-1, Suita, Osaka, 565-0871, Japan

7-57

Global transcriptional response of the methylotrophic yeast Hansenula polymorpha to environmental stresses caused by cadmium and sulfur starvation.
Min Jeong Sohn (1), Jeong-Nam Park (1), Sang Yup Lee (2), Hyun Ah Kang (1)
(1) Metabolic Engineering Lab, KRIBB, 52 Eoeun-dong,Yuseong, Daejeon, 305-333, South Korea; (2) Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, South Korea

7-58

Activation of mating MAP kinase by Kluyveromyces lactis killer protein.
Hiroko Kitamoto
Genetic diversity, NIAS, 2-1-2 Kannondai, Tsukuba, 305-8602, Ibaraki, Japan

7-59

Cdc25 protein overexpression reveals an unexpected nuclear localization.
Renata Tisi (1), Francesco Brunetti (2), Enzo Martegani (1)
(1) Dip. Biotecn. e Bioscienze, Universita' di Milano Bicocca, P.za della Scienza 2, Milan, 20126, Italy; (2) Dip. Biologia, Universita' di Milano, via Celoria 26, Milan, Italy

7-60

Ribosomal resistance to trichothecene toxins.
Shamsozoha Abolmaali (1), Doris Lucyshyn (1), Bernd Busch (1), Amir Mousavi (2), Rudolf Mitterbauer (1), Marc Lemmens (3), Hermann Buerstmayr (3), Gerhard Adam (1)
(1) Institute of Applied Genetics, BOKU Vienna, Muthgasse 18, Vienna, 1190, Austria; (2) National Research Center for Genetic, Engineering and Biotechnology (NRCGEB) Tehran, Iran; (3) BOKU Department for Agrobiotechnology (IFA Tulln), Tulln, Austria

7-61

Characterization of plant glucosyltransferases by heterologous expression in yeast.
Wolfgang Schweiger (1), Brigitte Poppenberger (1), Doris Lucyshyn (1), Franz Berthiller (2), Rainer Schuhmacher (2), Rudolf Krska (2), Rudolf Mitterbauer (1), Gerhard Adam (1)
(1) Institute of Applied Genetics, BOKU Vienna, Muthgasse 18, Vienna, 1190, Austria; (2) BOKU - Department for Agrobiotechnology, CD Laboratory for Mycotoxin Research, Tulln, Austria

7-62

Translational regulation in the unfolded protein response of Saccharomyces cerevisiae.
Tom Payne (1), Colin Hanfrey (2), Anthony J. Michael (2), Simon V. Avery (1), David B. Archer (1)
(1) School of Biology, University of Nottingham, University Park, Nottingham, NG7 2RD, UK; (2) Division of Food Safety Science, Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK.

7-63

Studying on zinc toxicity and antioxidant defense mechanism in Schizosaccharomyces pombe.
C. Tarhan, M. Pekmez and A. Topal Sarikaya

7-64

Yeast response to plasma membrane perturbation by chitosan.
Anna Zakrzewska (1), Andre Boorsma (1), Stanley Brul (2), Klaas J. Hellingwerf (1), Frans M. Klis (2)
(1) SILS Microbiology, University of Amsterdam, Nieuwe Achtegracht, Amsterdam, 1018WV, The Netherlands; (2) Molecular Biology and Microbial Food Safety, Swammerdam Institute of Life Sciences, Nieuwe Achtergracht 166, 1018WV Amsterdam, The Netherlands

7-65

Role of stress-defence enzymes in yeast colony development.
Michal Cap (1), Libuse Vachova (2), Zdena Palkova (1)
(1) Dept.Genetics and Microbiology, Charles University, Vinicna 5, Prague, 128 44, Czech Republic; (2) Institute of Microbiology, Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic

7-66

A role of amino acids in the ammonia signalling of yeast colonies.
Marketa Begany (1), Libuse Vachova (1), Zdena Palkova (2)
(1) Institute o Microbiology, Academy of Sciences, Videnska 1083, Prague 4, CZ, 14220, Czech Republic; (2) Department of Genetics and Microbiology, Charles University,Vinicna 5, 128 44 Prague 2, Czech Republic

7-67

Snf1 protein kinase is regulated by a phosphorylation-independent mechanism.
Daniel Bosch Ibáñez, Christer Larsson, Lena Gustafsson, Joakim Norbeck
Chemical & Biological Sciences, Chalmers University of Techno., Medicinaregatan 9C, Gothenburg, 40530, Sweden

7-68

Cell physiology and proteomic profile of yeast cells grown under limiting calcium concentration.
Valeria Mapelli (1), Stefano Busti (1), Rossella Sanvito (2), Farida Tripodi (1), Lorenzo Querin (1), Fulvio Magni (2), Alain Van Dorsselaer (3), Marzia Galli Kienle (2), Lilia Alberghina (1), Marco Vanoni (1)
(1) Biotecnologie e Bioscienze, University of Milano Bicocca, Piazza della Scienza, Milano, 20126, Italy; (2) University of Milano Bicocca, DIMESAB, Monza, Italy; (3) CNRS, University Louis Pasteur, LSMBO, Strasbourg, France

7-69

Relationship of DFG16 to the Rim101p pH-response pathway in Saccharomyces cerevisiae and Candida albicans.
Karen Barwell, Aaron Mitchell
Department of Microbiology, Columbia University, 701 W. 168th St, New York, NY, 10027, USA

7-70

Methionine sulfoximine-treatment and carbon starvation elicit Snf1-independent phosphorylation of the transcriptional activator Gln3 in Saccharomyces cerevisiae.
Jennifer J. Tate, Rajendra Rai, Terrance G. Cooper
Molecular Sciences, University of Tennessee, 858 Madison Ave., Memphis, TN, 38163, U.S.A.

7-71

In vivo specificity of Ure2 protection from heavy metal ion and oxidative cellular damage in Saccharomyces cerevisiae.
Rajendra Rai, Terrance G. Cooper
Molecular Sciences, University of Tennessee, 858 Madison Ave., Memphis, TN, 38163, U.S.A.

7-72

Relationship between calcium metabolism and sugar-induced activation of plasma membrane ATPase in the yeast Saccharomyces cerevisiae.
Maria José M. Trópia (1), Anamaria S. Cardoso (1), Renata Tisi (2), Luciano G. Fietto (1), Juliana L. R. Fietto (1), Enzo Martegani (2), Ieso M. Castro (1), Rogelio L. Brandão (1)
(1) Farmácia, Universidade Fed. Ouro Preto, LBCM/NUPEB/ICEB II, Ouro Preto, MG, 35400-000, Brazil; (2) Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, Piazza della Scienza 2, Milan, 20126, Italy

7-73

Using ethanol-tolerant mutants and microarrays to chacterize the molecular response of Saccharomyces cerevisiae to ethanol stress.
Dragana Stanley (1), Sarah Fraser (1), Paul J. Chambers (1), Peter Rogers (2), Grant A. Stanley (1)
(1) School of Molecular Sciences, Victoria University, Hoppers Lane, Werribee, 3030, Australia; (2) Carlton and United Breweries Ltd., 4-6 Southhampton Crescent, Abbotsford, Victoria, Australia 3067

7-74

Polyol-induced osmotic stress system - the application to study yeast osmostress signaling.
Dagmara Medrala, Sara Karlgren, Bodil Nordlander, Nina Pettersson, Stefan Hohmann
Dept. of CMB/Microbiology, Göteborg University, Medicinaregatan 9E, Göteborg, 40530, Sweden

7-75

Comparison between S. cerevisiae and K. lactis reveals divergent evolution of the Snf1-Cat8-Sip4 signaling network.
Jorrit-Jan Krijger (1), Christian Wiedemuth (1), Lydia Schild (1), Godefroid Charbon (2), Rosaura Rodicio (3), Karin D. Breunig (1)
(1) Institute of Genetics, University of Halle, Weinbergweg 10, Halle, D-06099, Germany; (2) Laboratoire de Genetique Moleculaire, Facultes Universitaires Notre-Dame de la Paix, 5000 Namur, Belgium; (3) Bioquimica y Biologica Molecular, Universidad de Orvieto, Spain

7-76

Ptr3, a WD40- and Ring/U-box-containing protein involved in amino-acid signalling, is down-regulated in a SCFGrr1-dependent manner.
Cathy Jean, Antonio Urrestarazu, Fadi Abdel-Sater, Bruno André
ULB, IBMM, Pr. Jeener & Brachet, Gosselies, 6041, Belgium

7-77

γ-irradiation activates the S. cerevisiae oxidative stress regulator Yap1 in a H₂O₂-dependent manner.
Mikael Molin (1), Jean-Philippe Renault (2), Gilles Lagniel (1), Michel Toledano (3), Serge Pin (2) and Jean Labarre (1)
(1) Laboratoire de PhysioGénomique, SBGM/DBJC/DSV; (2) Laboratoire de Radiolyse, SCM-URA 331 CNRS/DRECAM/DSM; (3) Laboratoire Stress Oxydants et Cancers, SBMS/DBJC/DSV CEA/Saclay, F-911 91 Gif-sur-Yvette Cedex, France

7-78

Survivalin the absence of oxygen: A comparative assessment of the transcriptionalresponse of S. cerevisiae and the obligatory-aerobic filamentousfungus Trichoderma reesei for energy production under transit anoxicconditions.
Ari J. S. Ferreira, Eric D. Bonaccorsi, Marluce Mantovani, Hamza El-Dorry
Departmentof Biochemistry, Institute of Chemistry, Avenida Prof. Lineu Prestes 748, Sao Paulo SP 05508-900 Sao Paulo, Brazil

Workshop & Poster Abstracts Topic 8 - What do conventional models not tell us?

8-1

The External Mitochondrial Alternative Dehydrogenases from Kluyveromyces Lactis.
Nuria Tarrío (1), María Esperanza Cerdán (2), María Isabel González Siso (2)
(1) Department of Cellular and Molecular Biology. University of A Coruña. Campus A Zapateira s/n. Spain; (2) Cellular and Molecular Biology, University of A Coruña, Campus A Zapaterira, A Coruña, 15071, Spain

8-2

Readthrough of nonsense mutations within the ERG1 gene restores growth and leads to pleiotropic phenotypes in Saccharomyces cerevisiae.
Christoph Ruckenstuhl, Christoph Nusshold, Christoph Jandl, Silvia Lang, Olga Oskolkova, Petra Koefinger, Andreas Uellen, Friederike Turnowsky
IMB, Karl - Franzens Universitaet, Universitaetsplatz 2, Graz, 8010, Austria

8-3

Everything you always wanted to know about wine yeasts but were afraid to ask.
Julia Oh (1), Duccio Cavalieri (2)
(1) Stanford Genome Technology Center, 855 California Avenue, Palo Alto, CA 94304, USA; (2) Department of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy

8-4

Prion cross-talk: an approach for the identification of new prions.
Irina L. Derkatch, Catherine J. Potenski, Nancy K. Horstman
Dept. Microbiology, NYU School of Medicine, 550 First Ave MSB217, New York, NY, 10016, USA

8-5

Evolutionary origin of multidrug transporter genes in five related yeast species.
Yvetta Gbelska (1), Jorrit-Jan Krijger (2), Karin D. Breunig (2)
(1) Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, 842 15 Bratislava,Slovak Republic; (2) Institut für Genetik, Martin-Luther Universität Halle-Wittenberg, 06099 Halle, Germany

8-6

Saccharomyces kluyveri is becoming a popular organism to study degradation of several anti-cancer drugs.

Jure Piskur (1), Gorm Andersen (1), Birgit Andersen (1), Michael Sandrini (1), Stina Lundgren (2), Olof Bjoernberg (1), Klaus D. Schnackerz (1), Doreen Dobritzsch (2)

Workshop & Poster Abstracts Topic 9 - Protein biosynthesis, maturation, modification and degradation

9-1

Respiratory deficiency of sup45 mutants may be compensated epigenetically.
Kirill Osipov, Kirill Volkov, Ludmila Mironova
Dep. of Genetics and Breeding, St.Petersburg State University, Universitetskaya emb, St. Petersburg, 199034, Russian Federation

9-2

The Nha1 antiporter from the Saccharomyces cerevisiae is an extremely stable plasma membrane protein.
Hana Flegelova (1), Rosine Haguenauer-Tsapis (2), Hana Sychrova (1)
(1) Dpt. of Membrane Transport, Institute of Physiology, Videnska 1083, Prague 4, 142 20, Czech Republic; (2) Institut Jacques Monod-CNRS, Universites Paris VI and VII, 2 place Jussieu, 75251 PARIS Cedex 05, France

9-3

Expression of mitochondrial release factor in relation to respiratory competence in yeast.
Joanna Towpik (1), Jan Kutner (1), Magdalena Boguta (1)
(1) Institute Biochemistry and Biophysics PAS, Pawinskiego 5A, 02-106 Warsaw , Poland

9-4

Extrachromosomal antisuppressor [ ASP⁺] of yeast Saccharomyces cerevisiae is localized in nucleus.
Veniamin A. Startsev, Sergey P. Zadorsky, Julia V. Sopova, Sergey G. Inge-Vetchtomov
Dep. of Genetics and Breeding, St.Petersburg State University, Universitetskaya emb, St. Petersburg, 199034, Russia

9-5

SUMOylation of budding yeast kinetochore proteins.
Ben Montpetit, Phil Hieter
Michael Smith Laboratories, University of British Columbia, #301-2185 East Mall, Vancouver, BC, V6T 1Z4, Canada

9-6

The role of N-terminal region of eRF3 from Saccharomyces cerevisiae in translation termination.
Valery Urakov, Natalia Kochneva-Pervukhova, Igor Valouev, Michael Ter-Avanesyan
Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya, Moscow, 121552, Russia

9-7

Mitochondrial Lon proteases recognize and initially cleave folded endogenous substrates.
Gabriela Onrovič ová (1), Tong Liu (2), Kamal Singh (2), Oleksandr Gakh (3), Dusan Pereč ko (1), Jiří Janata (3), Nathalia Parkhomenko (3), Zvi Granot (4), Joseph Orly (4), Carolyn Suzuki (2), Eva Kutejová (1)
(1) Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravska cesta 21, 845 51 Bratislava, Slovak Republic; (2) UMDNJ- New Jersey Medical School, Department of Biochemistry and Molecular Biology, 185 South Orange Avenue, Newark, NJ 07103 USA; (3) Institute of Microbiology, Academy of Sciences of the Czech Republic Vídeň ská 1083, 142 20 Prague 4, Czech Republic; (4) Department of Biological Chemistry The Alexander Silberman Institute of Life Sciences The Hebrew University of Jerusalem Jerusalem 91904, Israel

9-8

Identification and characterization of binding motifs that contribute to NAC:ribosome complex formation.
Renee D. Wegrzyn, Thomas Rauch, Diana Hofmann, Elke Deuerling
Universitaet Heidelberg, ZMBH, Im Neuenheimer Feld, Heidelberg, 69120, Germany

9-9

Interactions of eIF3 Subunit NIP1/c with eIF1 and eIF5 Promote Pre-initiation Complex Assembly and Regulate Start Codon Selection.
Leos Valasek (1), Klaus Nielsen (2), Fan Zhang (2), Christie Fekete (2), Alan Hinnebusch (2)
(1) Division of Cellular and Molecular Microbiology , Institute of Microbiology AS CR, Prague, 142 20, the Czech Republic; (2) Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA

9-10

Hsp70's Role in Prion Propagation- Mutational Analysis Reveals the Importance of the ATPase Domain.
Harriet M. Loovers, Gary W. Jones
Biology, NUI Maynooth, Maynooth, Co. Kildare, 00000, Ireland

9-11

Stabilization of Gcn4p in starved S. cerevisiae cells is mediated by dissociation of Pho85p/Pcl5p complex and depends on Pho81p and Pcl7p.
Katrin Bömeke, Ralph Pries, Virginia Korte, Gerhard H. Braus
Microbiology and Genetics, Georg-August University, Grisebachstr. 8, Goettingen, 37077, Germany

9-12

Identification of abnormal protein substrates of the yeast Rsp5 ubiquitin ligase under stress conditions.
Hiroyuki Hiraishi (1), Takashi Shimada (2), Taka-aki Sato (2), Hiroshi Takagi (1)
(1) Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, Fukui, 910-1195, Japan; (2) Proteomics Research Center Life Science Laboratory, Life Science Bu, Shimadzu Co., 3-17-1 Azuma, Tsukuba, Ibaraki 305-0031, Japan

9-13

Manifestation and propagation of prion-like antisuppressor determinant [ ISP⁺]in Saccharomyces cerevisiae depend on SIS2 gene functions.
Anna Aksenova (1), Ivan Munoz (2), Kirill Volkov (1), Joaquin Arino (1), Ludmila Mironova (1)
(1) Dept of Genetics, St. Petersburg University, Universitetskaya nab, St. Petersburg, 199034, Russia; (2) Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, Bellaterra 08193, Barcelona, Spain

9-14

Selection of protease-deficient strains of fission yeast Schizosaccharomyces pombe for production of protease-susceptible proteins.
Alimujiang Yidiresi, Kewei Bi, Hideki Tohda, Hiromichi Kumagai, Yuko Giga-Hama
ASPEX Division, Asahi Glass Co., Ltd., 1150 Hazawa Kanagawa, Yokohama, 221-8755, Japan

9-15

Cytosolic and nuclear pools of Pan1p are required for growth of yeast on oleate medium.
Joanna Kaminska, Monika Wysocka, Iwona Smaczynska de Roij, Joanna Rytka, Teresa Zoladek
Genetics, Institute Biochem Biophys PAS, Pawinskiego 5A, Warsaw, 02-106, Poland

9-16

Sumoylation of a nucleolar protein Ebp2 in Saccharomyces cerevisiae.
Chiharu Shirai, Masanobu Nariai, Keiko Mizuta
Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashihiroshima, 739-8528, Japan

9-17

Manifestation of the prion like determinant [ ISP⁺] depends on mutations in SUP45 gene.
Nikolai Rovinskii, Anna Aksenova, Ludmila Mironova
Department of Genetics, St. Petersburg State Universit, Univ. nab. 7/9, St. Petersburg, 199034, Russia

9-18

Magnesium homeostasis in yeast: The N-terminal domain of Alr1p is essential for its Mg²⁺-specific degradation.
Marcin Wachek, Michael Aichinger, Rudolf Schweyen, Anton Graschopf
MFPL, Department of Genetics, University of Vienna, Dr. Bohrgasse 9, Vienna, A-1030, Austria

9-19

eRF3 translation termination factors: from yeast to mammals.
Oleg Tarasov (1), Vadim Schepachev (1), Alexandra Petrova (1), Svetlana Moskalenko (1), Alexej Kostygov (2), Ekaterina Rodchenkova (2), Natalia Abramson (2), Galina Zhouravleva (1)
(1) Dept. of Genetics and Breeding, St.Petersburg State University, Universitetskaja 7/9, St. Petersburg, 199034, Russian Federation; (2) Zoological Institute, Russian Academy of Sciences, Universitetskaya emb. 1, St. Petersburg, 199034, Russian Federation

9-20

Mouse mGSPT2 protein causes guanidine hydrochloride-dependent nonsense-suppression in yeast Saccharomyces cerevisiae.
Alexandra Petrova, Olga Zemlyanko, Galina Zhouravleva
Dept. of Genetics and Breeding, St.Petersburg State University, Universitetskaya emb, Saint-Petersburg, 199034, Russian Federation

9-21

Incompatibility of prion [ PSI⁺] and mutations in SUP45 gene of the yeast Saccharomyces cerevisiae.
Denis Kiktev, Tatyana Galkina, Sergey Inge-Vechtomov, Galina Zhouravleva
Dept. of Genetics and Breeding, St Petersburg State University, Universitetskaya nab, Saint-Petersburg, 199034, Russian Federation

9-22

Spontaneous entering of GFP-TNFR1 fusion protein into the peroxisomes of Pichia pastoris.
Gorazd Hribar (1), Ana Lenassi Zupan (1), Vladka Gaberc Porekar (1), Viktor Menart (2)
(1) Biosynthesis&Biotransformation, National Inst. of Chemistry, Hajdrihova 19, Ljubljana, SI-1000, Slovenia; (2) Lek Pharmaceuticals, d.d., Verovskova 57, SI-1000 Ljubljana, Slovenia, National Inst. of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia

9-23

Search for modifications of translation termination accuracy in sup45 nonsense mutants of yeast Saccharomyces cerevisiae.
Svetlana Moskalenko (1), Olga Murina (2), Galina Zhouravleva (2)
(1) 199034 St.Petersburg Universitetskaya nab.7/9, Russia; (2) Genetics and Selection, St.Petersburg State University, Universitetskaya 7/9, St.Petersburg, 199034, Russian Federation

9-24

Rot1p is a glycosylated membrane protein localized in the ER-nuclear envelope network.
M. Angeles Juanes, J. Carlos Igual, M. Carmen Bañó
Bioquimica i Biol. Molec., Universitat de València, Doctor Moliner 50, Burjassot, 46100, Spain

9-25

Structural Basis of the Infectivity of HET-s Prion Protein from Podospora anserina.
Marie-Lise Maddelein (1), Christianne Ritter (2), Ansgar Siemer (3), Thorsten Luehrs (2), Mattias Ernst (3), Beat Meier (3), Sven Saupe (1), Roland Riek (2)
(1) IBGC, CNRS UMR 5095, 1 rue Camille Saint Saens , 33077 Bordeaux 2, France; (2) The Salk Institute , 10010 North Torrey Pines Road, La Jolla CA 92037 USA; (3) Eidgenoessische Technische Hochschule, Zuerich, Switzerland

9-26

Analysis of the 26S proteasome biogenesis using temperature-sensitive RP-mutants of Saccharomyces cerevisiae.
Erika Isono, Naoko Kamata, Akio Toh-e
Dept. of Biological Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-0033, Japan

9-27

The expression of RPN2 encoding a non-ATPase subunit of the 19S RP of the 26S yeast proteasome is regulated by proteasome.
Akio Toh-e, Tomoko Oguchi, Erika Isono, Yasushi Saeki, Wng I Chung
Biological Sciences, The University of Tokyo, 7-3-1 Hongo, Tokyo, 113-0033, Japan

9-28

Novel GCD genes with functions required for translation initiation.
Mercedes Tamame, Pilar Martin, Ruben Barrera, Diego Iglesias
Microbiology and Genetics, CSIC/University of Salamanca, Campus Unamuno, Salamanca, 37007, Spain

9-29

Mutations in SUP35 gene coding for translation termination factor eRF3 lead to NMD defect.
Svetlana Chabelskaya (1), Michel Philippe (2), Galina Zhouravleva (1)
(1) Department of Genetics and Breeding, St Petersburg State University, Universitetskaya emb. , 7/9, 199034, St Petersburg, Russia, Université de Rennes 1, CNRS UMR 6061, IFR 140, 2 av. Pr. Léon Bernard 35043 Rennes Cedex, France; (2) Université de Rennes 1, CNRS UMR 6061, IFR 140, 2 av. Pr. Léon Bernard 35043 Rennes Cedex, France

9-30

Identification of proteins differentially translated by ribosomal stalk mutants supports a translation regulatory role for the yeast ribosome.
Antonio Jiménez-Díaz, Jesús Revuelta, Miguel Remacha, Juan P.G. Ballesta
Centro de Biología Molecular, U.A.M. and C.S.I.C., Canto Blanco, Madrid, 28049, Spain

9-31

The SFP1 is a potential host gene for yeast prion-like determinant [ ISP⁺].
Sofia Rodionova, Tatiana Rogoza, Kirill Volkov, Ludmila Mironova
Department of Genetics, St. Petersburg University, Univ. nab. 7/9, St.Petersburg, 199034, Russia

9-32

[PIN⁺] -independent aggregates contain yeast protein Ade2 fused with the prion-forming domain of Sup35 protein.
Aleksey Pogoda, Vasiliy Manukhov, Vladimir Alenin, Andrey Borchsenius, Sergey Inge-Vechtomov
Department of Genetics, St. Petersburg University, University emb. 7/9, St. Petersburg, 199034, Russian Federation

9-33

Phenotypic characterization of sup35 mutations obtained in vitro.
Andrey S. Borchsenius, Andrey I. Ermakov, Sergey G. Inge-Vechtomov
Department of Genetics, St. Petersburg University, University emb. 7/9, St. Petersburg, 199034, Russian Federation

9-34

Chimeric Sup35SP protein forms aggregates [ PIN⁺] - independently in Saccharomyces cerevisiae.
Julia Sopova, Dmitry Akhmedov, Sergey Zadorsky, Sergey Inge-Vechtomov
Genetics and Breeding, Saint Petersburg University, University emb., Saint Petersburg, 199034, Russian Federation

9-35

Structural analysis of N-linked oligosaccharides assembled on recombinant proteins secreted from Yarrowia lipolytica Yloch1 and Yloch1 Ylmnn4 mutants.
Min Hee Choi (1), Yunkyung Song (1), Hyun Ah Kang (2), Jeong-Yoon Kim (1)
(1) Department of Microbiology, Chungnam National University, Yuseong-gu, Daejeon, 305-764, Korea; (2) Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea

9-36

Activation of Sapp1p isoenzyme from Candida parapsilosis.
Jiří Dostál, Helena Dlouhá, Petr Maloň , Iva Pichová, Olga Hrusková-Heidingsfeldová
Dept.of Protein Biochemistry, ÚOCHB, Flemingovo nám.2, Prague, 16610, Czech Republic

9-37

The DNA damage inducible UbL-UbA protein Ddi1 participates in Mec1-mediated degradation of Ho endonuclease.
Ludmila Kaplun, Regina Tzirkin, Dina Raveh
Life Sciences, Ben Gurion University, P.O.Box 653, Beersheba, 84105, Israel

9-38

Influence of [ PSI⁺]-factor on complementation of ade2 alleles in Saccharomyces cerevisiae.
Sergey G. Inge-Vechtomov, Alexander M. Zekhnov, Andrey S. Borchsenius
Department of Genetics, St. Petersburg University, University emb., 7/9, St. Petersburg, 199034, Russian Federation

9-39

The gerontogene Sir2p is required for active retention of damaged proteins during cytokinesis.
Nika Erjavec, Thomas Nyström
CMB Microbiology, Gothenburg University, Medicinaregatan 9c, Gothenburg, 40530, Sweden

Workshop & Poster Abstracts Topic 10 - Organelles biogenesis and inheritance

10-1

Mechanism of mitochondrial adenine nucleotide homeostasis.
Xiaowen Wang, Blanka Kucejova, Xin Jie Chen
Department of Molecular Biolog, University of Texas Southweste, 5323 Harry Hines Blv, Dallas, TX, 75390-9148, USA

10-2

The effect of phosphatidylcholine depletion on Gut2 function in yeast.
Pieter Rijken, Ben de Kruijff, Toon de Kroon
Biochemistry of Membranes, Utrecht University, Padualaan 8, Utrecht, 3584 CH, The Netherlands

10-3

Saccharomyces cerevisiae COX24 codes for a protein required for COX1 processing.
Mario H. Barros (1), Alexander Tzagoloff (2)
(1) Genetica, UNESP, Rv Domingos Sartori, Botucatu, SP, 18607-741, Brazil; (2) Columbia University - Amsterdam Av., New York, NY 10027 USA

10-4

Vacuolar turnover of mitochondria in cells lacking ATG gene products.
Dalibor Mijaljica (1), Carlos Rosado (1), Rodney J. Devenish (1), Mark Prescott (1)
(1) Biochemistry, Monash University, Wellington Road, Melbourne, 3800, Australia

10-5

Mutations in yeast mitochondrial RNA polymerase genes partially suppress RNA processing defects due to the lack of mitochondrial degradosome function.
Agata Rogowska (1), Olga Puchta (1), Anna M. Czarnecka (1), Aneta Kaniak (2), Piotr P. Stepien (1), Pawel Golik (1)
(1) Department of Genetics, Warsaw University, Pawinskiego 5A, Warsaw, 02-106, Poland; (2) Institute of Biochemistry and Biophysics PAS, Pawinskiego 5A, 02-106 Warsaw, Poland

10-6

Inp1p is a peroxisomal membrane protein required for peroxisome inheritance in Saccharomyces cerevisiae.
Monica Fagarasanu (1), Andrei Fagarasanu (1), Yuen Yi C. Tam (1), John D. Aitchison (2), Richard A. Rachubinski (1)
(1) Cell Biology, University of Alberta, 114 St - 89 Ave, Edmonton, AB, T6G2H7, Canada; (2) The Institute for Systems Biology, Seattle, Washington 98103, USA

10-7

Inp2p is a peroxisomal protein required for the Myo2p-driven transport of peroxisomes during cell division in Saccharomyces cerevisiae.
Andrei Fagarasanu (1), Monica Fagarasanu (1), Gary Eitzen (1), John D. Aitchison (2), Richard A. Rachubinski (1)
(1) Cell Biology, University of Alberta, 114 St - 89 Ave, Edmonton, AB, T6G2H7, Canada; (2) The Institute for Systems Biology, Seattle, Washington 98103, USA

10-8

Integration of the peroxisome into the physiology of Saccharomyces cerevisiae.
Daniel Lockshon, Lauren Surface, Brian Kennedy
Biochemistry, University of Washington, Box 357350, Seattle, WA, 98195, USA

10-9

In vitro activity and substrate specificity of the mitochondrial degradosome complex.
Michal Malecki (1), Robert Jedrzejczak (2), Piotr P. Stepien (1), Pawel Golik (1)
(1) Department of Genetics, Warsaw University, Pawinskiego 5A, Warsaw, 02-106, Poland; (2) National Cancer Institute/MCL/Argonne National Laboratory, Biosciences Division, 9700 South Cass Avenue, Bldg.202, Rm.Q142, Argonne, IL 60439, USA

10-10

The role of cdk pho85p in mitochondrial distribution in yeast S. cerevisiae.
Anastasia Fizikova (1), Elena Sambuk (2), Ksenia Zakharova (2)
(1) 197373,44/2 Aviakonstruktorov pr. ap.120, St.Petersburg; (2) Genetics and Selection, St-Petersburg State University, Universitetskaya, St.-Petersburg, 199034, Russian Federation

10-11

Novel insights into mitochondrial c-type cytochrome maturation: a membrane-bound flavoprotein and a candidate assembly complex.
Delphine Bernard (1), Sophie Cheruel (2), Sabeeha Merchant (3), Bernard Guiard (1), Patrice Hamel (3)
(1) Genetics of Cellular Functions, CNRS - CGM, Ave de la terrasse, Gif-sur-Yvette, 91198, FRANCE; (2) IBBMC, UMR8619 - Bât 430 - Université de Paris-Sud , 91405 ORSAY CEDEX, France; (3) Department of Chemistry and Biochemistry- UCLA- , 607 Charles E. Young Drive East , Los Angeles, CA 90095-1569 , USA

10-12

Relationship between control of mitochondrial volume and mitochondrial cation homeostasis: Insights from a genomic screen for nigericin-resistant mutants.
Blanka Kucejova (1), Martin Kucej (1), Silvia Petrezselyova (2), Lenka Abelovska (1), Dominka Fricova (1), Adriana Rycovska (1), Jozef Nosek (1), Lubomir Tomaska (2)
(1) Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 84215 Bratislava, Slovakia; (2) Department of Genetics, Comenius University, Mlynska dolina B-1, Bratislava, 84215, Slovakia

10-13

A new function of the ER: contribution to peroxisome formation.
Dominic Hoepfner (1), Danny Schildkegt (2), Ineke Braakman (2), Peter Philippsen (3), Henk F. Tabak (2)
(1) Biomedical Research, Novartis Pharma AG, Postfach, Basel, 4002, Switzerland; (2) Department of Cellular Protein Chemistry, Utrecht University, Padualaan 8, NL-3548 CH Utrecht, The Netherlands; (3) Lehrstuhl für Angewandte Mikrobiologie, Biozentrum, Universität Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland

10-14

Molecular interactions of the mitochondrial tim12 pivotal subunit with the substrate and small tim partners.
Carine De Marcos Lousa (1), Catherine Baud (2), Girogos Panayotou (3), Kostas Tokatlidis (2)
(1) IMBB-FORTH, P.O. Box 1527 Vassilika Vouton, 71110 Heraklion, Crete, Greece; (2) IMBB, FORTH, Vasslika Vouton,1527, Heraklion, 71110, Greece; (3) Flemming institute, B.S.R.C. "Alexander Fleming", P.O. Box 74145, 16602 Varkiza Greece

10-15

Investigation of the physiological and molecular functions of the mitochondrial carrier Sal1p.
Juraj Laco, Igor Zeman, Jordan Kolarov
Dept. of Biochemistry, Comenius University, Mlynska dolina CH-1, Bratislava, 84215, Slovakia

Workshop & Poster Abstracts Topic 11 - Regulation of gene expression and chromatin remodelling

11-1

Mode of action for Mss11p: a central factor in the FLO11 transcription machinery.
Dewald van Dyk (1), Isak S. Pretorius (2), Florian F. Bauer (1)
(1) Inst. for Wine Biotechnology, Stellenbosch University, Victoria Street, Stellenbosch, 7600, South Africa; (2) The Australian Wine Research Institute, Waite Road, Urrbrae, Adelaide SA 5064, South Australia, Australia

11-2

Point mutation in C128 subunit of the yeast RNA polymerase III compensates deffect in regulation of the tRNA biosynthesis in the absence of Maf1.
Malgorzata Ciesla, Karol Balicki, Magdalena Boguta
Genetics, IBB PAS, Pawinskiego 5A, Warsaw, 02-106, Poland

11-3

Effect of the Prion [ PSI⁺] on Expression of COX5b Gene Encoding Subunit of Cytochrome Oxidase in Saccharomyces cerevisiae.
Agnieszka Jankowska (1), Isabelle Hatin (2), Olivier Namy (2), Jean-Pierre Rousset (2), Magdalena Boguta (1)
(1) Institute of Biochemistry and Biophysics, PAS, Pawinskiego 5a, 02-106 Warsaw, Poland; (2) Institute of Genetics and Microbiology, University Paris-Sud, 91405 Orsay, France

11-4

Cap-binding translation initiation factor 4E from Candida albicans functionally complements the cdc33 mutation in Saccharomyces cerevisiae and confers temperature sensitivity to the heterologous host.
Zuzana Feketova (1), Vaclav Vopalensky (2), Tomas Masek (2), Martin Pospisek (2)
(1) Dep. of genetics and microbiology, Vinicna 5, Prague 2, 128 44 Czech Republic; (2) Dep. of genetics and microbiol, Charles University, PrfUK, Vinicna 5, Prague 2, CZ, 128 44, Czech Republic

11-5

Chromatin remodeling factors and the histone variant H2A.Z/Htz1p counteract in regulating FLO11 expression in Saccharomyces cerevisiae.
Claudia Fischer, Oliver Valerius, Gerhard H. Braus
Microbiology and Genetics, Georg-August-University, Grisebachstrasse 8, Goettingen, 37077, Germany

11-6

Chromatin structure of yeast Saccharomyces cerevisiae could be revealed by a new technique - Yeast Comet Assay.
Milena Kirilova, George Miloshev
Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad.G.Bonchev,bl.21, Sofia, 1113, Bulgaria

11-7

Constitutive expression of yeast phospholipid biosynthetic genes by variants of Ino2 activator defective for interaction with Opi1 repressor.
Willm-Thomas Heyken, Antje Repenning, Hans-Joachim Schueller
Dept. of Genetics, University of Greifswald, Jahnstrasse 15a, Greifswald, D-17487, Germany

11-8

Influence of low glycolytic activities in gcr1 and gcr2 mutants on the expression of other metabolic pathway genes in Saccharomyces cerevisiae.
Hiroshi Uemura, Hiromi Sasaki
Inst. for Biological Resources, AIST, Higashi 1-1-1, Tsukuba, Ib, 305-8566, Japan

11-9

New roles for the TFIIS cleavage-elongation factor of RNA Polymerase II.
Maxime Wery (1), Elena Shematorova (2), Pierre Thuriaux (1)
(1) SBGM, CEA-Saclay, Bat. 144, Gif-sur-Yvette, F-91191, France; (2) Shemyakin-Ovchinnikov Institute, Russian Academy of Sciences, Moscow, Russia

11-10

Analyses of transcriptional regulation by the nuclear actin-related protein Arp6 of Saccharomyces cerevisiae.
Takahito Yoshida (1), Hitoshi Iwahashi (2), Kazuto Kugou (3), Kunihiro Ohta (3), Masahiko Harata (1)
(1) Molecular and Cell Biology, Tohoku University, Tsutsumidori 1-1, Aoba-ku Sendai, 981-8555, Japan; (2) Human Stress Signal Research Center, Natl Inst of Advanced Industrial Science and Technology, Central-6, Higashi 1-1, Tsukuba 305-8566, Japan; (3) Genetics Dynamics Research Unit-Lab, RIKEN, Hirosawa 2-1, Wako-shi 351-0198, Japan

11-11

Functional interaction between RNAP II and the Ssu72 CTD phosphatase.
Mariela Reyes-Reyes, Michael Hampsey
Biochemistry, UMDNJ-GSBS, 675 Hoes Lane, Piscataway, NJ, 08854, USA

11-12

Pub1 interacts with Nab2 in an RNA-independent manner in S. cerevisiae.
Luciano H. Apponi (1), Michelle T. Harreman (2), Anita H. Corbett (2), Sandro R. Valentini (1)
(1) Dept. of Biological Sciences, School of Pharmacy - UNESP, Rod. Araraquara-Jau, Araraquara, SP, 14801-902, Brazil; (2) Dept. of Biochemistry, Emory University School of Medicine, Clifton Rd NE, Atlanta, GA, 30322, USA

11-13

Analysis of two DAS genes encoding dihydroxyacetone synthase in the methylotrophic yeast Pichia methanolica.
Tomoyuki Nakagawa, Shuki Fujimura, Takashi Ito, Mika Chikui, Takashi Miyata, Tatsuro Miyaji, Noboru Tomizuka
Dept. Food Sci. & Technol., Tokyo Univ. of Agriculture, Yasaka 196, Abashiri, 099-2493, Japan

11-14

Analysis of FLD1 gene encoding glutathione-dependent formaldehyde dehydrogenase in the methylotrophic yeast Pichia methanolica.
Shuki Fujimura (1), Tomoyuki Nakagawa (1), Takashi Ito (1), Mika Chikui (1), Tatsuro Miyaji (1), Hiroya Yurimoto (2), Nobuo Kato (2), Yasuyoshi Sakai (2), Noboru Tomizuka (1)
(1) Dept. Food Sci. & Technol., Tokyo Univ. of Agriculture, Yasaka 196, Abashiri, 099-2493, Japan; (2) Div. Appl. Life Sci., Kyoto Univ., Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan

11-15

SWI/SNF- GCN5 -Dependent chromatin remodeling determines induced expression of GDH3, one of the paralogous genes responsible for ammonium assimilation and glutamate biosynthesis in Saccharomyces cerevisiae.
Lina Riego, Amaranta Avendaño, Cristina Aranda, Cecilia Ishida, Guillermo Romero, Alicia Gonzalez
Genetica Molecular, Instituto Fisiologia Celular, Circuito Exterior, Mexico City, 04510, Mexico

11-16

Chromatin organization and combinatorial action of Gln3p and Gcn4p in the UGA3 - GLT1 bidirectional promoter of Saccharomyces cerevisiae.
Cristina Aranda, Cecilia Ishida, Lourdes Valenzuela, Alexander DeLuna, Lina Riego, Paola Ballario, Alicia Gonzalez
Genetica Molecular, Instituto Fisiologia Celular, Circuito Exterior, Mexico City, 04510, Mexico

11-17

Gcn4 participates in nitrogen catabolite repression in Saccharomyces cerevisiae.
Guillermo Romero, Silvia Cordero, Cristina Aranda, Alexander DeLuna, Lina Riego, Alicia Gonzalez
Genetica Molecular, Instituto Fisiologia Celular, Circuito Exterior, Mexico City, 04510, Mexico

11-18

Arrest of DNA synthesis prevents G₂/M specific transcription by interfering with Ndd1 function.
Helene Klug, Gustav Ammerer
Department of Biochemistry, University of Vienna, Dr. Bohrgasse 9, Wien, A, 1030, Austria

11-19

Gene expression in the absence of Topoisomerases I and II in S. cerevisiae.
Maria L. Garcia Rubio, Andres Aguilera
Genetics, University of Seville, Av. Reina Mercedes 6, Seville, 41012, Spain

11-20

The role of histone deacetylation in transcriptional regulation of G2/M specific promoters.
Jiri Veis, Helene Klug, Manfred Koranda, Gustav Ammerer
Department of Biochemistry, University of Vienna, Dr. Bohrgasse 9, Vienna, 1030, Austria

11-21

Networks of gene regulation of one carbon metabolism in Saccharomyces cerevisiae.
Johnny Lee (1), Cristy Gelling (1), Geoff Kornfeld (1), Ian Dawes (1)
(1) Ramaciotti Centre for Gene Function Analysis & School of Biotechnology and Biomolecular Science, University of New South Wales, Sydney, NSW 2052, Australia

11-22

Cbf1-1p of Schizosaccharomyces pombe - testing of an ancestral partnership.
Martin Př evorovský, Jan Rynes, Tomás Grousl, Petr Folk, Frantisek Pů ta
Physiology and Dev. Biology, Fac. of Science, Charles Univ., Vinič ná 7, Praha 2, 128 00, Czech Republic

11-23

Role of the Sir3 and Orc1 BAH domains in transcriptional silencing.
Jessica Connelly (1), Peihua Yuan (1), Hao-Chi Hsu (2), Zhizhong Li (2), Rui-Ming Xu (2), Rolf Sternglanz (1)
(1) Biochemistry and Cell Biology, Stony Brook University, Life Sciences Bldg., Stony Brook, NY, 11794-5215, USA; (2) Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

11-24

Transcriptional regulation by the Cup9 DNA binding protein.
A. Irina Mutiu, Christopher J. Brandl
Biochemistry, University of Western Ontario, 1151 Richmond Street, London, ON, N6A5C1, Canada

11-25

The role of the histone methyltransferase Dot1 in gene silencing.
Floor Frederiks, Tibor van Welsem, Fred van Leeuwen
Cellular Biochemistry, Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, 1066 CX, The Netherlands

11-26

Mediator and SAGA coactivators are recruited independently by Met4 to its target promoters.
Christophe Leroy (1), Laetitia Cormier (2), Laurent Kuras (2)
(1) Centre Génétique Moléculaire, Bat.26 av. de la terrasse, 91 198 Gif-sur-Yvette Cedex France; (2) Centre Génétique Moléculaire, CNRS, av.de la terrasse, Gif-sur-Yvette, 91 198, France

11-27

Presumed pre-mRNA processing factor Prp45p of S. cerevisiae - analysis of the essential function.
Katerina Abrhamova, Tereza Simonova, Ondrej Gahura, Petr Folk, Frantisek Puta
Physiology and Dev. Biology, Charles University, Vinicna 7, Prague, 128 00, Czech Republic

11-28

The role of histones and their modifications in the heritability of cell function.
Kitty Verzijlbergen, Tibor van Welsem, Fred van Leeuwen
Cellular Biochemistry, Netherlands Cancer Institute, plesmanlaan 121, Amsterdam, 1066 CX, The Netherlands

11-29

MRN1 - encoding a novel mRNA binding protein - is a multicopy suppressor of RSC - Nhp6 synthetic lethality.
Louis Düring, Steen Holmberg
Department of Genetics, University of Copenhagen, Oesterfarimagsgade2A, Copenhagen, DK-1353, Denmark

11-30

Genome-wide expression profiling of carbon metabolism reveals an essential role of the protein kinase Snf1 in the regulation of methanol metabolism and peroxisome biogenesis in Hansenula polymorpha.
Doo-Byoung Oh (1), Ohsuk Kwon (1), Yong Kyung Kim (1), Yun Wi Oh (1), Cheol-Goo Hur (1), Gerd Gellissen (2), Sang Ki Rhee (1), Hyun Ah Kang (1)
(1) Metabolic Engineering Lab., KRIBB, 52 Eoeun-dong, Daejeon, 305-333, Korea; (2) Rhein Biotech GmbH, Eichsfelder Str. 11, 40595 Dusseldorf, Germany

11-31

Cellular arsenic stress responses mediated by YAP8.
Catarina Amaral (1), Regina Menezes (1), Frédéric Devaux (2), Claudina Rodrigues-Pousada (1)
(1) Stress and Genomics, ITQB, Apt127, OEIRAS, 2781-901, Portugal; (2) Laboratoire de Genetique Moleculaire, CNRS UMR 8541, Ecole Normale Superieure, 46 rue d'Ulm, 75230 Paris cedex 05, France

11-32

Characterization of jumonji-domain interacting proteins in yeast.
Susanna Tronnersjö, Christine Hanefalk, Gou-Zhen Hu, Niklas Nordberg, Darius Balciunas, Eva Murén, Hans Ronne
IMBIM and VBSG, Uppsala University and SLU, Box 582, Uppsala, SE-751 23, Sweden

11-33

Med31 (Soh1) Mediator subunit and TFIIS elongation factor play redundant roles in transcription initiation at GAL1 promoter.
Benjamin Guglielmi, Julie Soutourina, Cyril Esnault, Michel Werner
SBGM, CEA/Saclay, Bâtiment 144, Gif Cedex, 91191, France

11-34

The bHLH transcription activator Sck1, controlled by casein kinase I, binds to the promoter of the glucose permease gene RAG1 of K. lactis.
Helen Neil, Micheline Wésolowski-Louvel, Marc Lemaire
Microbiology and Genetic, University Claude Bernard Lyon, 10 rue Dubois, Villeurbanne, 69622, France

11-35

Functional characterization of Yaf9p, a component of the chromatin remodelling complexes NuA4 and SWR, with suppressor genetics and molecular tools.
Michele M. Bianchi (1), Eugenia Piccinni (1), Ania Chelstowska (2), Rodolfo Negri (1), Laura Frontali (1)
(1) Cell and Developmental Biology, University of Rome La Sapienza, p.le Aldo Moro, Rome, 00185, Italy; (2) Dept. of Genetics, Institut of Biochemistry and Biophysiscs, Warshaw, Poland

11-36

The RRPE and PAC promoter motifs function in the transcriptional regulation of the genes in the yeast ribosome and rRNA biosynthesis (RRB) regulon.
Michael McAlear, Christopher Wade
Molecular Biology and Biochem., Wesleyan University, 237 Church St, Middletown, CT, 06459, USA

11-37

Analysis of transcriptional profiles of S. pombe sep10 and sep15 mutant strains, which encode subunits of the Mediator complex.
Ida Miklos (1), Zsolt Szilagyi (1), Zsigmond Benko (1), Klara Enczi (1), Gyula Batta (1), Matthias Sipiczki (1), Jurg Bahler (2)
(1) Department of Genetics, University of Debrecen, Egyetem tér 1, Debrecen, 4010, Hungary; (2) Wellcome Trust Sanger Institute, Hingston, Cambridge CB10 1SA, United Kingdom

11-38

Mating type information regulates telomeric gene silencing in the yeast Saccharomyces cerevisiae.
Yuval Mazor, Martin Kupiec, Anat Krauskopf
Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel

11-39

Hir1 and Ssn6 coregulators function in combination on the Mac1-dependent CTR1 transcription.
Alexandra Voutsina, Kalliopi Gkouskou, Despina Alexandraki
Biology-Yeast Mol. Genetics, FORTH-IMBB and Univ. of Crete, P.O. BOX 1527, Heraklion-Crete, 71110, Greece

11-40

The role of RSC in 2 micron plasmid maintenance.
Annette A. Alcasabas, James A. H. Murray
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, United Kingdom

Workshop & Poster Abstracts Topic 12 - Addressing human diseases, aging and apoptosis with yeast

12-1

Identification of sequences responsible for lost of activity of the human Tre2 oncogene product by functional complementation of the yeast msb3 msb4 mutant.
Christelle Bizimungu, Daniel Portetelle, Micheline Vandenbol
Unite de Biol Anim et Microb, FUSAGx, Ave M. Juin, 6, Gembloux, 5030, Belgium

12-2

Six fold Life Span Extension by Double Mutations in SIR2 and SCH9 or RAS2/CYR1.
Paola Fabrizio, Valter Longo
Biological Sciences, University of Southern California, 3715 McCLintock, Los Angeles, CA, 90089, USA

12-3

A mammalian phospholipase A₂ in yeast: a global view.
Mojca Mattiazzi (1), Uros Petrovič (1), Joseph L. DeRisi (2), Igor Krizaj (1)
(1) Biochem. and Mol. Biol., Jozef Stefan Institute, Jamova 39, Ljubljana, SI-1000, Slovenia; (2) University of California, San Francisco, CA, USA

12-4

TRIP6, a novel substrate of mammalian Snf1 orthologue, AMP-activated protein kinase.
M. Carmen Solaz-Fuster, Jose V. Gimeno-Alcañiz, Pascual Sanz
Inst. Biomedicina de Valencia, C.S.I.C., Jaime Roig, 11, Valencia, E-46010, Spain

12-5

Conformational memory preserved in a weak-to-strong or strong-to-weak [ PSI⁺] conversion during transmission to Sup35 prion variants.
Colin Crist, Hiroshi Kurahashi, Toru Nakayashiki, Yoshikazu Nakamura
Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan

12-6

Mutations in AAC2, equivalent to human adPEO-associated ANT1 mutations, lead to defective oxidative phosphorylation in Saccharomyces cerevisiae and affect mitochondrial DNA stability.
Iliana Ferrero (1), Flavia Fontanesi (1), Luigi Palmieri (2), Pasquale Scarcia (2), Tiziana Lodi (1), Claudia Donnini (1), Anna Maria Viola (1), Anna Limongelli (3), Valeria Tiranti (3), Massimo Zeviani (3)
(1) Department of Genetics Anthrop, University of Parma, Parco Area delle Sci, Parma, 43100, Italy; (2) Department of Pharmaco-Biology, University of Bari, 70125 Bari, Italy; (3) Division of Molecular Neurogenetics, National Neurological Institute "C. Besta" 20126 Milano

12-7

A screen to identify yeast genes involved in apoptosis.
Alexandra Schauer, Christoph Ruckenstuhl, Heide Knauer, Heike Fussi, Elisabeth Hulla, Laura Leitner, Cornelia Rumpf, Julia Spielhofer, Kai-Uwe Fröhlich
IMB, Karl - Franzens Universitaet, Universitaetsplatz 2, Graz, A, 8010, Austria

12-8

Saccharomyces cerevisiae as a model for the study of the mammalian PI3K-PTEN-Akt pathway.
Isabel Rodriguez-Escudero (1), Françoise M. Roelants (2), Cesar Nombela (1), Jeremy Thorner (2), Maria Molina (1), Victor J. Cid (1)
(1) Dpto. de Microbiologia II, Universidad Complutense, Pza. Ramon y Cajal, Madrid, 28040, Spain; (2) Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, California 94720 USA

12-9

Saccharomyces cerevisiae as a host cell model for the study of enteropathogenic Escherichia coli (EPEC) virulence factors.
Isabel Rodriguez-Escudero (1), Philip R. Hardwidge (2), Cesar Nombela (1), B. Brett Finlay (2), Victor J. Cid (1), Maria Molina (1)
(1) Dpto. de Microbiologia II, Universidad Complutense, Pza. Ramon y Cajal, Madrid, 28040, Spain; (2) Michael Smith Laboratories. #301 - 2185 East Mall. University of British Columbia. Vancouver, British Columbia. Canada, V6T 1Z3

12-10

Oligopeptide repeats of Sup35 and [PSI+] variability.
Irina S. Shkundina (1), Vitaly V. Kushnirov (1), Mick F. Tuite (2), Michael D. Ter-Avanesyan (1)
(1) Experimental Cardiology, Cardiology Research Centre, 3rd Cherepkovskaya, Moscow, 121552, Russia; (2) Research School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK

12-11

Using budding yeast as a genetic model to identify the underlying cellular defects in the human primary immunodeficiency Wiskott-Aldrich Syndrome.
Alan Munn (1), Gang Ren (1), Juan Wang (2), Bostjan Kobe (3), Ross Brinkworth (3), Barbara Winsor (4)
(1) Inst for Molecular Bioscience, The University of Queensland, 306 Carmody Road, St Lucia, Q, 4072, Australia; (2) School of Pharmacy, The University of Wisconsin, Maddison, WI, 53705-2222, USA; (3) Department of Biochemistry and Molecular Biology, The University of Queensland, St Lucia, Q, 4072, Australia; (4) "odèles Levure de Pathologies Humaines, FRE2375 du CNRS, IBMC, 67084 Strasbourg, France

12-12

Analysis of ribosomal protein S19, a factor involved in human Diamond-Blackfan Anemia.
Britta Meyer, Markus Buchhaupt, Peter Kötter, Karl-Dieter Entian
Institute of Microbiology, J.W.G. University Frankfurt, Marie-Curie-Str.9, Frankfurt am Main, 60439, Germany

12-13

Does a sensescence factor matter?
Renata Zadrag, Grzegorz Bartosz, Tomasz Bilinski
Institute of Biology, Univeristy of Rzeszow, Rejtana 16, Rzeszow, 35 310, Poland

12-14

Neutral lipid homeostasis in the yeast Saccharomyces cerevisiae.
Julia Petschnigg, Christoph Kurat, Regina Leber, Klaus Natter, Harald Scholz, Heimo Wolinski, Nermina Malanovic, Katrin Fantur, Astrid Gruber, Kathrin Winkler, Sepp D. Kohlwein
Biochemistry, IMB, Schubertstrasse 1, Graz, 8010, Austria

12-15

Yeast as a tool for in vivo study of human mitochondrial tRNA mutations: a study of nuclear suppressors in yeast.
Laura Frontali, Silvia Francisci, Cristina De Luca, Veronica Morea, Romina Oliva, Anna Tramontano
Cell and Developmental Biolog, University of Rome La Sapienza, P.ale A. Moro 5, Rome, 00185, Italy

12-16

A novel rapid in vitro and in vivo coupled screen for anti-prion compounds put in light Tannic acids as a modulator of amyloidogenesis.
Mona Boyé-Harnasch, Christophe Cullin
IBGC, CNRS Université Bordeaux2, 1 rue Camille saint, Bordeaux, 33000, France

12-17

Analysis of Shigella effector proteins in Saccharomyces cerevisiae.
John Rohde, Philippe Sansonetti, Claude Parsot
PMM, Institut Pasteur, 28 rue du Dr. Roux, Paris, 75724, France

12-18

The cloning and expression of human ALS mutant Cu,Zn SOD into Schizosaccharomyces pombe.
Semian Karaer (1,2), Cagatay Tarhan (1), Aysegul Topal Sarikaya (1,2)
(1) Molecular Biology and Genetics, Istanbul University, Vezneciler, 34118 Istanbul, Turkey; (2) Research and Application Centre for Biotechnology and Genetic Engineering, 34118 Istanbul, Turkey

12-19

Role of YGR076c in yeast mother cell-specific aging.
Gino Heeren, Stefanie Jarolim, Mark Rinnerthaler, Peter Laun, Elfriede Palle, Michael Breitenbach
Department of Cell Biology, University of salzburg, Helbrunnerstrasse 34, Salzburg, 5020, Austria

12-20

Functional dissection of sequence-specific NKX2-5 mutations associated with human heart septation defects using a yeast-based system.
Alberto Inga (1), Stella Marie Reamon-Buettner (2), Juergen Borlak (2), Michael A. Resnick (3)
(1) Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA; Laboratory of Experimental Oncology B, Department of Translational Oncology, National Cancer Research Institute, IST, Genoa, Italy; (2) Department of Drug Research and Medical Biotechnology, Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany; (3) Chromosome Stability Section, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA

12-21

Functional analysis of Saccharomyces cerevisiae HSP31 gene, homolog of human DJ-1.
Arkadiusz Micialkiewicz (1), Adrianna Skoneczna (2), Marek Skoneczny (1)
(1) Department of Genetics, IBB PAS, Pawinskiego 5a, Warsaw, 02-106, Poland; (2) Department of Mutagenesis and DNA Repair, IBB PAS, Pawinskiego 5A, Warsaw, 02-106, Poland

12-22

TCTP (YKL056c), a highly conserved eukaryotic gene functionally interacting with both the cytoskeleton and mitochondria.
Michael Breitenbach (1), Mark Rinnerthaler (1), Elfriede Palle (1), Simona Perju (1), Peter Laun (1), Gino Heeren (1), Stefanie Jarolim (1), Frank Madeo (2)
(1) Dept. of Cell Biology, University of Salzburg, Hellbrunnerstrasse 3, Salzburg, 5020, Austria; (2) IMB, Karl-Franzens University, Universitaetsplatz 2, A-8010 Graz, Austria

12-23

The relation of resistance/sensitivity to heat shock and the mother cell specific life span of S. cerevisiae.
Stefanie Jarolim (1), Gabriel Perrone (2), Ian W. Dawes (2), Michael Breitenbach (1), Peter Laun (1)
(1) Dept. of Cell Biology, University of Salzburg, Hellbrunnerstrasse 3, Salzburg, 5020, Austria; (2) School of Biotechnology and Biomolecular Sciences , University of New South Wales , Sydney 2052 NSW Australia

12-24

Protein carbonylation as a marker of aging: studying the influence of the chromatin deubiquitinating enzyme Ubp10.
Maurizio Bèttiga (1), Luciano Calzari (1), Ivan Orlandi (1), Carlo Marvelli (1), Lilia Alberghina (1), Thomas Nyström (2), Marina Vai (1)
(1) Biotechnology and Biosciences, Università Milano-Bicocca, piazza della Scienza, Milan, 20126, Italy; (2) Department of Cell and Molecular Biology-Microbiology, Göteborg University, Medicinaregatan 9C, 405 30 Göteborg, Sweden

12-25

Cellular role of the mitochondrial K⁺/H⁺ exchange activity mediated by MKH1.
Karin Nowikovsky, Rudolf J. Schweyen
MFPL, Department of Genetics, University of Vienna, Dr. Bohr Gasse 9, Vienna, 1030, Austria

12-26

Studying the functions of human genes in S. cerevisiae.
Zorana Vujovic (1), Kuangyu Yen (1), Jill Wishart (1), Manda Gent (1), Mike Osborn (2), Ross Miller (2), Nianshu Zhang (1), Stephen Oliver (1)
(1) Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester, M13 9PT, UK; (2) Molecular Immunology Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK

12-27

Functional analysis of human signal peptidase in yeast.
Kuangyu Yen, Nianshu Zhang, Stephen Oliver
Faculty of Life Science, The University of Manchester, Oxford Road, Manchester, M15 6HR, United Kingdom

12-28

A study of SPR-2: a human transcription factor which suppresses yeast ipl1.
Jill A. Wishart (1), Nianshu Zhang (1), Michael Osborn (2), Ross Miller (2), Andrew Hayes (1), Thomas Grotkjaer (1), Leanne Wardleworth (1), Stephen G. Oliver (1)
(1) The Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester, M13 9PT, United Kingdom; (2) Molecular Immunology Programme, The Babraham Institute, Babraham, Cambridge, CB2 4AT, United Kingdom

12-29

Differentiation in yeast colonies: Ammonia regulated localisation of the cell death.
Libuse Vachova (1), Zdena Palkova (2)
(1) Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic; (2) Department of Genetics and Microbiology, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic

12-30

Genome-wide analysis of the effects of protein prenylation inhibition on gene expression in yeast cells.
Giampiero Porcu (1), Cathal Wilson (2), Antonella Ragnini (1)
(1) Dept. of Biology, University of Rome, Tor Vergat, Viale della Ricerca, Rome, 00133, Italy; (2) Department of Cell Biology and Oncology Consorzio Mario Negri Sud, S.M. Imbaro Chieti, Italy

12-31

A Genome-wide Screen Reveals Deletion of Tor Pathway Components Extends Chronological Life Span.
Trey Powers (1), Matt Kaeberlein (1), Brian K. Kennedy (2), Stan Fields (3)
(1) Department of Genome Sciences, University of Washington, Box 357360, Seattle, WA, 98195, United States; (2) Department of Biochemistry, University of Washington, United States; (3) Departments of Genome Sciences and Medical Genetics, University of Washington; The Howard Hughes Medical Institute, United States

12-32

Reconstruction of human human pathogenic mutations in complex I from the obligate aerobic yeast Yarrowia lipolytica.
Stefan Kerscher, Aurelio Garofano, Ljuban Grgic, Noushin Kashani-Poor, Pamela Okun, Ulrich Brandt
Biochemie I - Haus 26, Uniklinik Frankfurt, Theodor-Stern-Kai 7, Frankfurt/Main, 60590, Germany

12-33

Apoptosis-like cell death of Schizosaccharomyces pombe induced by hydrogen peroxide or acetic acid is accompanied with mitochondrial fragmentation and it is prevented by Bcl-x_L expression.
Vladimír Pevala, Jordan Kolarov
Department of Biochemistry, Comenius University, Mlynska dolina CH-1, Bratislava, 842 15, Slovakia

12-34

The effects of some human pathogenic mutations in mitochondrial tRNA genes can be studied and suppressed in yeast.
Céline Besagni (1), Cristina De Luca (2), Silvia Francisci (2), Laura Frontali (2), Monique Bolotin-Fukuhara (1)
(1) Laboratoire de Génétique Moléculaire, Bat. 400, Université Paris Sud, Orsay, France; (2) Dept. Cell and Dev. Biology, University of Rome La Sapienza, P.le Aldo Moro 5, Rome, It, 00185, Italy

12-35

A mutation in the ERG3 gene causes selective resistance to the polyene nystatin but not to amphotericin B in S. cerevisiae.
Katarína Mazánová, Vlasta Klobucníková, Peter Kohút, Peter Griac, Ivan Hapala
Dept. Bioenergetics, Institute of Animal Biochemistry and Genetics, Moyzesova 61, Ivanka pri Dunaji, 90028, Slovak Republic

12-36

AAC is involved in the permeabilization of the outer mitochondrial membrane during acetic acid-induced cell death in Saccharomyces cerevisiae.
Clara Pereira (1), Stephén Manon (2), Maria João Sousa (1), Manuela Côrte-Real (1)
(1) Departamento de Biologia, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal; (2) C.N.R.S./Université de Bordeaux 2, France

12-37

Uth1p is involved in the autophagic degradation of mitochondria.
Ingrid Kissova (1), Maika Deffieu (2), Giséle Velours (2), Bénédicte Salin (2), Jacques Schaeffer (2), Stéphen Manon (2), Nadine Camougrand (2)
(1) Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Mlynska dolina CH-1, 84215 Bratislava, Slovak Republic; (2) UMR5095 CNRS/Université de Bordeaux 2, 33700 Bordeaux, France

Workshop & Poster Abstracts Topic 13 - Vesicular transport, lipids and membrane remodeling

13-1

Multiple functions of the vacuolar sorting protein Ccz1p in Saccharomyces cerevisiae.
Marta Hoffman-Sommer, Aleksandra Jaszcza, Joanna Rytka, Róza Kucharczyk
Department of Genetics, IBB PAS, Pawinskiego 5a, Warsaw, 02-106, Poland

13-2

The yeast mitochondrial phosphatidylethanolamine-synthesizing machinery interacts with components governing function and morphology of mitochondria.
Sabine Rosenberger, Ruth Nebauer, Guenther Daum
Institute of Biochemistry, Graz University of Technology, Petersgasse 12/2, Graz, A-8010, Austria

13-3

Cellular processes affected by a phospholipase A₂ in yeast.
Uros Petrovic (1), Mojca Mattiazzi (1), Jernej Sribar (1), Heimo Wolinski (2), Harald Scholz (2), Sepp D. Kohlwein (2), Igor Krizaj (1)
(1) Dept. of Biochem. & Mol. Biol., Jozef Stefan Institute, Jamova 39, Ljubljana, SI-1000, Slovenia; (2) Institute of Molecular Biosciences, University of Graz, Schubertstrasse 1, Graz, A-8010, Austria

13-4

Enhanced level of Pis1p in S. cerevisiae allows for better growth of cells with unsaturated fatty acids deficiency caused by defect in Rsp5 ubiquitin ligase.
Pawel Kaliszewski (1), Thierry Ferreira (2), Beata Gajewska (3), Anna Szkopinska (4), Thierry Berges (2), Teresa Zoladek (1)
(1) Department of Genetics, Institute of Biochemistry and Biophysics PAS, Warsaw, Poland; (2) Universite de Poitiers, Faculte des Sciences, Genetique de la Levure UMR CNRS, Poitiers, France; (3) Department of Biochemistry, Faculty of Medicine, Medical University of Warsaw; (4) Department of Lipid Biochemistry, Institute of Biochemistry and Biophysics PAS, Warsaw, Poland

13-5

Characterization of protein-protein interactions in the Yeast Conserved Oligomeric Golgi (COG) complex.
Vladimir Lupashin, Pierre Fotso, Yuliya Koryakina, Elena Suvorova, Oleksandra Pavliv, Arnold Tsiomenko
Physiology , slot 505, UAMS, 200 South Cedar St, Little Rock, AR, 72205, USA

13-6

Identification and characterization of Hansenula polymorpha ELO1 and ELO2 genes required for chain elongation of very long-chain fatty acids.
Phatthanon Prasitchoke (1), Yoshinobu Kaneko (1), Toshihiro Nagao (2), Yuji Shimada (2), Satoshi Harashima (1)
(1) Dept. Biotechnology, Grad. Schl. Eng., Osaka Univ., Yamadaoka 2-1, Osaka, 565-0871, Japan; (2) Osaka Municipal Tech. Res. Inst., Osaka, 536-8553, Japan

13-7

Targeting sequences of the yeast Pmp3p/Sna1p and it homologues.
Wojtek Pokrzywa, Jean-François Hochstenbach, Pierre Morsomme
Biochimie Physiologique, University of Louvain, Croix du Sud 2/20, Louvain-la-Neuve, 1348, Belgium

13-8

The organisation of the preautophagosomal structure during the Cvt pathway requires Trs85 (Gsg1), a component of the TRAPP complexes.
Khuyen Meiling-Wesse (1), Ulrike D. Epple (1), Henning Barth (1), Anika Appelles (1), Christiane Voss (1), Eeva-Liisa Eskelinen (2), Michael Thumm (1)
(1) Biochemistry II, University of Goettingen, Heinrich-Düker-Weg12, Goettingen, 37073, Germany; (2) Institute of Biochemistry, University of Kiel; Otto Hahn Platz 9; D-24118 Kiel, Germany

13-9

Cloning and expression of flax fad2 desaturase in yeast.
Anna Krasowska, Dorota Dziadkowiec, Anna Polinceusz, Marcin Lukaszewicz
Inst. of Genetics & Microbiol., Wroclaw University, Przybyszewskiego 63, Wroclaw, 51-148, Poland

13-10

A novel SNARE complex implicated in vesicular traffic to the prevacuolar compartment.
Blair Doneske, Kirilee Wilson, Travis Rice-Stitt, James McNew
Biochemistry and Cell Biology, Rice University, 5100 Main Street, Houston, TX, 77005, United States of America

13-11

Identification and characterization of a novel family of lipases required for steryl ester hydrolysis in Saccharomyces cerevisiae.
René Köffel, Rashi Tiwari, Linda Corbino, Roger Schneiter
University of Fribourg, Institute of Biochemistry, Chemin du Musée 5, Fribourg, 1700, Switzerland

13-12

Saccharomyces cerevisiae mutants with altered levels of the sphingolipid class of M(IP)2Cs display differential sensitivities to the antifungal plant defensin DmAMP1.
Karin Thevissen (1), Isabelle François (1), An Aerts (1), Jola Idkowiak-Baldys (2), Yang-Ju Im (2), Jon Takemoto (2), Johnny Roosen (3), Joris Winderickx (3), Sabina Accardo (4), Dirk De Vos (4), Bruno Cammue (1)
(1) CMPG, Katholieke Universiteit Leuven, Kasteelpark Arenberg, Heverlee, 3360, Belgium; (2) Dept. of Biology, Utah State University, Logan, UT, US; (3) Laboratory of Functional Biology, K.U. Leuven, Heverlee, Belgium; (4) Centre for Surface Chemistry and Catalysis, K.U. Leuven, Heverlee, Belgium

13-13

Arf1p, Chs5p, and the ChAPs are required to bud specialized vesicles from the Golgi in Saccharomyces cerevisiae.
Mark Trautwein (1), Robert Gauss (1), Joern Dengjel (2), Anne Spang (1)
(1) Friedrich-Miescher-Laboratory, Max-Planck-Society, Spemannstr. 39, Tübingen, 72076, Germany; (2) Department of Immunology, Institute of Cell Biology, University of Tuebingen, Auf der Morgenstelle 15, D-72076, Germany

13-14

Functional genomics of monensin sensitivity in yeast.
Gunilla Barmark, Marie Gustavsson, Jimmy Larsson, Eva Murén, Hans Ronne
Uppsala University and SLU, IMBIM and VBSG, Box582, Uppsala, SE-751 23, Sweden

13-15

Roles of phosphoinositide-bis-phosphate in Saccharomyces cerevisiae filamentous growth.
Isabelle Guillas, Robert A. Arkowitz
Inst. Sig., Dev. Bio, & Cancer, CNRS UMR 6543, Parc Valrose, Nice, 06108, France

13-16

A highly conserved binding site in VAP for the FFAT motif of lipid binding proteins.
Chris Loewen, Tim Levine
Division of Cell Biology, Institute of Ophthalmology, Bath Street, London, EC1V 9EL, UK

13-17

Sfh1p, homologue of the yeast phosphatidylinositol/phosphatidylcholine transfer protein Sec14p.
Roman Holic, Dana Tahotna, Peter Griac
Bioenergetics, Inst of Animal Biochem Genet, Moyzesova 61, Ivanka pri Dunaji, 900 28, Slovakia

13-18

Effect of cerulenin on neutral lipid metabolism in Saccharomyces cerevisiae.
Zuzana Mrózová, Tibor Czabany, Martin Valachovic, Ivan Hapala
Dept. Bioenergetics, Institute of Animal Biochemistry and Genetics, Moyzesova 61, Ivanka pri Dunaji, 90028, Slovak Republic

13-19

Identification of Fungal Sphingolipid C9-Methyltransferases by Phylogenetic Profiling.
Philipp Ternes (1), Petra Sperling (1), Sandra Albrecht (1), Stephan Franke (2), James M. Cregg (3), Dirk Warnecke (1), Ernst Heinz (1)
(1) Biozentrum Klein Flottbek und Botanischer Garten, Universität Hamburg, Ohnhorststr. 18, 22609 Hamburg, Germany; (2) Organische Mikroanalytik, Universität Hamburg, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany; (3) Keck Graduate Institute, 535 Watson Drive, Claremont, CA 91711, U.S.A.

Workshop & Poster Abstracts Topic 14 - Membrane transport, receptors and sensors

14-1

Molecular chaperons of SMF and Zn+2 metal ion transporters.
Noa Alumot (1), Adiel Cohen (1), Nathan Nelson (2)
(1) Biochemistry, Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel; (2) Department of Biochemistry, The George S. Wise Faculty of Life Sciences, Tel Aviv, University, Tel Aviv 69978, Israel

14-2

Targetting of the S. cerevisiae Na⁺/H⁺ antiporter Nha1 to the plasma membrane depends on the final step in ergosterol biosynthesis.
Katerina Malinska, Hana Sychrova
Dept. Membrane Transport, Institute of Physiology CAS, Videnska 1083, Prague 4, 142 20, Czech Republic

14-3

S. cerevisiae Kha1p is not a plasma membrane K⁺/H⁺ antiporter.
Lydie Maresova, Hana Sychrova
Dept. Membrane Transport, Inst. Physiol., CzAcadSci, Videnska 1083, Prague 4, 142 20, Czech Republic

14-4

Isolation of the NHA1 gene from two Debaryomyces hansenii strains.
Klara Velkova, Hana Sychrova
Dept. of Membrane Transport, Institute of Physiology CAS, Videnska 1083, Prague 4, 142 20, Czech Republic

14-5

Indentification of amino-acid residues important for the substrate specificity range of yeast plasma membrane Na⁺/H⁺-antiporters.
Olga Zimmermannova, Martin Zavrel, Hana Sychrova
Membrane Transport, Institute of Physiology, Videnska 1083, Prague 4 - Krc, 14220, Czech Republic

14-6

Riboflavin uptake in S. cerevisiae is catalysed by the monocarboxylate transorter homolog Mch5p.
Petra Reihl, Juergen Stolz
Dept. of Cell Biology, Regensburg University, Universitaetsstr. 31, Regensburg, D-93040, Germany

14-7

Characterisation of two putative vacuolar yeast sugar permeases.
Carsten Fruehbeis, Eckhard Boles
Institut fuer Mikrobiologie, J. W. Goethe-Universitaet, Marie-Curie-Str. 9, Frankfurt/Main, 60439, Germany

14-8

Characterisation of the hexose transporter-packaging chaperone Gsf2.
Doreen Ochmann, Tanja Hamacher, Eckhard Boles
Institut fuer Mikrobiologie, J.W. Goethe Universitaet, Marie-Curie-Straße 9, Frankfurt am Main, 60439, Germany

14-9

Connection between the glucose sensor Rag4 and the KlRgt1 repressor in Kluyveromyces lactis.
Micheline Wésolowski-Louvel, Martina Hnatova, Stéphane Rolland, Marc Lemaire
UMR5122, UCBL/CNRS/INSA, 10, rue Dubois, Villeurbanne, 69622, France

14-10

Maltotriose utilization in lager strains: MTT1 encodes a maltotriose transporter.
Judith Dietvorst (1), John Londesborough (2), Yde Steensma (3)
(1) Institute of Biology Leiden, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands; (2) VTT Biotechnology, P.O. Box 1500, FIN-02044, Finland; (3) Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands

14-11

sn-Glycerol-3-phosphate transport in Saccharomyces cerevisiae.
Yulia G. Popova, Elena Lonati, Johan M. Thevelein
Molecular Microbiology, KULeuven, VIB, Kasteelpark Arenberg, Leuven, B-3001, Belgium

14-12

Characterising functional domains in a Saccharomyces cerevisiae maltotriose transporter.
Annel Smit (1), Isak S Pretorius (2), Ricardo R Cordero Otero (1)
(1) Viticulture Oenology, Inst. Wine Biotechnology, Victoria street, Stellenbosch, 7600, South Africa; (2) The Australian Wine Research Institute, Adelaide, Australia

14-13

Cytoplasmic leucine inhibits extracellular amino acid sensing by Ssy1p, suggesting non-transporting, transporter-like sensor mechanism.
Boqian Wu, Kim Ottow, Peter Poulsen, Richard F. Gaber, Eva Albers, Morten C. Kielland-Brandt
C. Lab., Carlsberg Laboratory, Gl Carlsberg Vej 10, Copenhagen Valby, DK-2500, Denmark

14-14

Dual regulation of methionine transport by ubiquitination in Saccharomyces cerevisiae.
Alexandra Menant, Régine Barbey, Dominique Thomas
Centre génétique moléculaire, CNRS, Av de la Terrasse, Gif-sur-Yvette, 91198, France

14-15

A high-conductance channel mediates Mg²⁺ transport in S. cerevisiae mitochondria.
Julian Weghuber (1), Rainer Schindl (2), Christoph Romanin (2), Rudolf J. Schweyen (1)
(1) Genetics, Vienna Biocenter, Dr. Bohrgasse 9, Vienna, A, 1030, Austria; (2) Institute for Biophysics, University of Linz, A-4040 Linz, Austria

14-16

Ammonia signalling in Saccharomyces cerevisiae colonies: Role of SPS sensor.
Karel Harant (1), Libuse Vachova (1), Zdena Palkova (2)
(1) Institute of Microbiology, AVCR, Videnska 1083, Prague 4, 142 20, Czech Republic; (2) Department of Genetics and Microbiology, Charles University, Vinicná 5, 128 44 Prague2, Czech Republic

14-17

Relationships between leucine and threonine transport in yeast.
James R. Mattoon (1), Margaret Hensley (1), Carlos A. Stella (2)
(1) Biotechnology Center, Univ.of Colorado,Colo. Springs, 1420 Austin Bluffs P, Colorado Springs, CO, 80933-7150, USA; (2) Biochemistry Dept., School of Medicine, Buenos Aires University, Buenos Aires, Argentina

14-18

Ato protein synthesis is induced in liquid yeast cultures.
Dita Strachotova (1), Libuse Vachova (1), Zdena Palkova (2)
(1) Institute of Microbiology, Academy of Sciences, Videnska 1083, Prague 4, 14220, Czech Republic; (2) Department of Genetics and Microbiology, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic

14-19

S. cerevisiae putative ammonium exporters Ato are localised in lipid rafts.
Marketa Ricicova, Zdena Palkova
Genetics and Microbiology, Charles University, Vinicna 5, Prague 2, 128 44, Czech Republic

14-20

Distinct V H⁺ ATPases pump H⁺ into the lumen of the ER and Golgi of Saccharomyces cerevisiae and energise their membranes.
Solange S. Samarão, Carlos E.S. Teodoro, Thais M. Granato, Anna L. Okorokova-Facanha, Lev A. Okorokov
LFBM/CBB, UENF, Alberto Lamego 2000, Campos Goytacazes, RJ, 28013602, Brazil

14-21

The role of proline uptake in yeast mitochondria and the feast-famine regime.
Maria Luigia Pallotta
Department of Health Sciences, University of Molise, Via De Sanctis, Campobasso, 86100, Italy

14-22

DhTRK and DhHAK, Two genes encoding for K⁺ transporters in Debaryomyces hansenii.
Catarina Prista (1), Juan Carlos González-Hernández (2), José Ramos (3), Maria Conceição Loureiro-Dias (2)
(1) ULHT, 1749-024 Lisboa, Portugal and DBEB-Instituto Superior de Agronomia, 1349-017 Lisboa, Portugal; (2) DBEB-Instituto Superior de Agronomia, 1349-017 Lisboa, Portugal; (3) Dep. Microbiología, Universidad de Córdoba, Campus de Rabanales, C6, E-14071 Córdoba, Spain

14-23

Cloning and expression of genes coding for glycerol transport in the salt-tolerant yeast Debaryomyces hansenii.
Juan Carlos González-Hernández (1), Catarina Prista (2), Maria Conceição Loureiro-Dias (1)
(1) Departamento de Botânica e Engenharia Biológica, Instituto Superior de Agronomia, 1349-017 Lisboa, Portugal; (2) Departamento de Botânica e Engenharia Biológica, Instituto Superior de Agronomia, 1349-017 Lisboa, Portugal and Universidade Lusófona de Humanidades e Tecnologias, 1749-024 Lisboa, Portugal

14-24

Two novel xylose/glucose transporter genes from Candida intermedia PYCC 4715.
Maria J. Leandro, Paula Gonçalves, Isabel Spencer-Martins
CREM, Biotechnology Unit, FCT, New University of Lisbon, Quinta da Torre, Caparica, 2829-516, Portugal

14-25

Influence of an additional genomic mutation on localisation and signalling capacity of several Gap1 alleles in Saccharomyces cerevisiae.
Ole Lagatie, Johan Thevelein
Dept. of Mol. Cell Biology, KULeuven, Kasteelp Arenberg 31, Leuven-Heverlee, 3000, Belgium

14-26

Analysis of the mKir2.1 channel activity in potassium influx defective Saccharomyces cerevisiae strains determined as changes in growth characteristics.
Guido Hasenbrink (1), Sarah Schwarzer (1), Lucie Kolocna (2), Jost Ludwig (3), Hana Sychrova (4), Hella Lichtenberg-Frate (1)
(1) Ag Molecular Bioenergetics, University of Bonn, Kirschallee 1, Bonn, 53115, Germany; (2) Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic; (3) Physiologisch-chemisches Institut, Universität Tübingen, Hoppe-Seyler-Str. 4, 72076 Tübingen, FRG; (4) Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic

14-27

Role of the Npr1 protein kinase in the regulation of the activity of the Mep1, Mep2 and Mep3 ammonium transporters in Saccharomyces cerevisiae.
Mélanie Boeckstaens, Bruno André, Anna Maria Marini
ULB, IBMM, Pr. Jeener & Brachet, Gosselies, 6041, Belgium

14-28

A specific transcriptional mechanism compensates for defects in particular multidrug resistance transporters by induction of their homologues of overlapping specificity.
Marcin Kolaczkowski (1), Anna Kolaczkowska (2), André Goffeau (3), Scott Moye-Rowley (4)
(1) Department of Biophysics, Wroclaw Medical University, Chalubinskiego 10, Wroclaw, PL-50-368, Poland; (2) Institute of Biochemistry and Molecular Biology, University of Wroclaw, Tamka 2, PL-50-137 Wroclaw, Poland; (3) Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix du Sud 2-20, B-1348 Louvain-la-Neuve, Belgium; (4) Department of Physiology and Biophysics, University of Iowa, 6-530 Bowen Science Bldg., Iowa City, IA 52242, USA

Workshop & Poster Abstracts Topic 15 - Nuclear architecture and transport

15-1

Cytosolic pool of Rsp5 ubiquitin ligase is sufficient for proper intracellular tRNA distribution in Saccharomyces cerevisiae.
Piotr Cholbinski (1), Malgorzata Lewandowska (1), Anita K. Hopper (2), Teresa Zoladek (1)
(1) Department of Genetics, IBB PAS, Warsaw, Poland; (2) The Pennsylvania State University College of Medicine, Hershey, PA, USA

15-2

The Ctf18 alternative RF-C complex positions yeast telomeres but does not affect their replication time.
Shin-ichiro Hiraga, Anne Donaldson
Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, U. K.

15-3

Under lock and key: Small DNA-protein complexes can present the gene keys to the gene locks composed from DNA.
Bogdan A. Kurchii (1)
(1) Institute of Plant Physiology and Genetics, 31/17 Vasylkivska St., 03022 Kiev, Ukraine

15-4

A subunit of RNA polymerase II Rpb4 is localized in the cytoplasmic P-bodies and is required for the decay of a specific class of mRNAs.
Rona Lotan, Liat Harel, Daniel Melamed, Lea Duek, Mordechai Choder
Molecular Microbiology, Technion - Israel Institute of, Efron, Haifa, 31096, Israel

15-5

Ethanol-responsive change in the localization of Rat8/Dbp5 in Saccharomyces cerevisiae : analysis of mutants that are hypersensitive to ethanol regarding Rat8-localization.
Shingo Izawa, Yoshiharu Inoue
Graduate School of Agriculture, Kyoto University, Gokasho, Uji, Kyoto, 6110011, Japan

15-6

The Role of the Yku70/80p Heterodimer in Maintaining Nuclear Architecture in Saccharomyces cerevisiae.
Marcus Marvin, Sue Hardy, Pawan Noel, Edward Louis
Genetics, University of Leicester, University Road, Leicester, LE1 7RH, England

15-7

The cytoplasmic AAA-protein Drg1p is essential for ribosome biogenesis in Saccharomyces cerevisiae.
Brigitte Pertschy, Gertrude Zisser, Eva Wehrschütz-Sigl, Ida van der Klei, Gregor Högenauer, Helmut Bergler
Molecular Biosciences, Karl Franzens University Graz, Universitätsplatz 2, Graz, 8010, Austria

15-8

Nuclear import and export of the transcriptional repressor Whi5.
Joy L. Nishikawa, Xiaojing Tang, Mike Tyers
Samuel Lunenfeld RschInstitute, Mount Sinai Hospital, 600 University Ave, Toronto, ON, M5G1X5, Canada

15-9

Karyopherin-specific translocation pathways through the nuclear pore complex.
David Goldfarb (1), Nataliya Shulga (1), Laura J. Terry (2), Susan Wente (2)
(1) Biology, University of Rochestser, 436 Hutchison Hall, Rochester, NY, 14627, USA; (2) 2Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232-8240 USA

15-10

Unloading of Condensin from rDNA is compromised in lte1 mutants in S. cerevisiae.

Workshop & Poster Abstracts Topic 16 - Bioinformatics corner: Tools and databases

16-1

YEASTRACT: a database to search for transcription regulatory associations in Saccharomyces cerevisiae.
Miguel C. Teixeira (1), Pedro Monteiro (2), Pooja Jain (2), Sandra Tenreiro (1), Alexandra R. Fernandes (3), Nuno P. Mira (1), Marta Alenquer (1), Ana T. Freitas (2), Arlindo L. Oliveira (2), Isabel Sá-Correia (1)
(1) Biological Sciences Research Group, CEBQ, Instituto Superior Tecnico, 1049-001 Lisbon, Portugal; (2) Knowledge Discovery and Bioinformatics/ Algorithms for Optimization and Simulation group, INESC-ID, Lisbon, Portugal; (3) Biological Sciences Research Group, CEBQ, Instituto Superior Tecnico, 1049-001 Lisbon, Portugal, Universidade Lusófona de Humanidades e Tecnologias, Campo Grande, 376, 1749-029 Lisboa, Portugal.

16-2

e-Fungi: An e-Science infrastructure for comparative functional genomics in fungal species.
Mike Cornell (1), Intikab Alam (1), M. Nedim Alpdemir (1), Darren Soanes (2), Han Min Wong (3), Magnus Rattray (1), Simon Hubbard (4), Nick Talbot (2), Brian Lings (3), David Hoyle (3), Norman W. Paton (1), Stephen G. Oliver (4)
(1) School of Computer Science, University of Manchester, Oxford Road, Manchester, M13 9PL, UK; (2) Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter, EX4 4PS, UK; (3) School of Engineering, Computer Science and Mathematics, University of Exeter, Harrison Building, North Park Road, Exeter, EX4 4QF, UK; (4) Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK

16-3

T-profiler: scoring the activity of pre-defined groups of genes using gene expression data.
Andre Boorsma (1), Barrett Foat (2), Daniel Vis (1), Frans Klis (1), Harmen Bussemaker (2)
(1) SILS-Microbiology, University of Amsterdam, Nwe achtergracht 166, Amsterdam, 1018 WV, Netherlands; (2) Department of Biological Sciences, Columbia University, New York, New York 10027, U.S.A.

16-4

Presenting the UniProt Knowledgebase - a comprehensive protein database, and Integr8 - a gateway to the bioinformatics information and analysis of complete proteomes.
Kati K.M. Laiho (1), Vivien Junker (2), Ivo Pedruzzi (2), Marc Feuermann (2), Amos Bairoch (2), Rolf Apweiler (1)
(1) EMBL Outstation, European Bioinformatics Inst., WellcomeTrust Campus, Cambridge, CB101SD, United Kingdom; (2) Swiss Institute of Bioinformatics, 1 rue Michel-Servet, Geneva 4, CH-1211, Switzerland

16-5

New genetic and physical interaction data in the Saccharomyces Genome Database.
Maria C. Costanzo (1), Rose Oughtred (2), Kara Dolinski (2), Teresa Reguly (3), Ashton Breitkreutz (3), Lorrie Boucher (3), Nizar Batada (3), Bobby-Joe Breitkreutz (3), Rama Balakrishnan (1), Karen R. Christie (1), Selina S. Dwight (1), Stacia R. Engel (1), Dianna G. Fisk (1), Jodi E. Hirschman (1), Eurie L. Hong (1), Robert Nash (1), Anand Sethuraman (1), Marek S. Skrzypek (1), Chandra L. Theesfeld (1), Gail Binkley (1), Qing Dong (1), Stuart Miyasato (1), Shuai Weng (1), David Botstein (2), Mike Tyers (3), J. Michael Cherry (1)
(1) Department of Genetics, Stanford University, 300 Pasteur Drive, Stanford, CA, 94305, USA; (2) Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA; (3) Samuel Lunenfeld Research Institute and University of Toronto, Toronto, ON, Canada

16-6

Saccharomyces cerevisiae Morphological Database.
Taro L. Saito, Jun Sese, Yoichiro Nakatani, Fumi Sano, Masashi Yukawa, Yoshikazu Ohya, Shinichi Morishita
Dept. of Computational Biology, Univ. of Tokyo, 5-1-5 Kashiwa-no-ha, Kashiwa, 277-8561, Japan

16-7

ScDSAT: The Saccharomyces cerevisiae DataSet Analysis Tool.
Mark Temple (1), Andy Lail (2), Abhirami Ratnakumar (2), Louis Lam (2), Beniamin Paduch (2), Mike Bain (1), Ian Dawes (1)
(1) Ramaciotti Centre for Gene Functional Analysis; (2) School of Biotechnology and Biomolecular Sciences, University of NSW, Sydney, NSW, 2052, Australia

16-8

The Swiss-Prot Fungal Proteome Annotation Program (FPAP).
Ivo Pedruzzi (1), Vivien Junker (1), Marc Feuermann (1), Kati K.M. Laiho (2), Rolf Apweiler (2), Amos Bairoch (1)
(1) Swiss Institute of Bioinformatics (SIB), CMU, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland; (2) EMBL Outstation - European Bioinformatics Institute (EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, United Kingdom

16-9

The maxd suite: tools for microarray experimental annotation, dissemination and analysis.
Michael H. Wilson (1), David Hancock (1), Andy Brass (1), Giles Verlarde (2), Douglas Kell (2), Stephen G. Oliver (3)
(1) School of Computer Science, The University of Manchester, Oxford Road, Manchester, M13 9PL, United Kingdom; (2) School of Chemistry, The University of Manchester, Faraday Building, PO Box 88, Sackville Street, Manchester, M60 1QD, United Kingdom; (3) Faculty of Life Sciences, The Michael Smith Building, The University of Manchester, Oxford Road, Manchester, M13 9PT, United Kingdom

16-10

Searching full-text journal articles at the Saccharomyces Genome Database.
Eurie L. Hong (1), Qing Dong (1), Rob Nash (1), Christopher Lane (1), Shuai Weng (1), Rama Balakrishnan (1), Karen R. Christie (1), Maria C. Costanzo (1), Kara Dolinski (2), Stacia R. Engel (1), Dianna G. Fisk (1), Jodi E. Hirschman (1), Rose Oughtred (2), Marek Skrzypek (1), David Botstein (2), J. Michael Cherry (1)
(1) Genetics, Stanford University, 300 Pasteur Drive, Stanford, CA, 94043, USA; (2) Department of Molecular Biology, Princeton University, Princeton, NJ 08544 USA

Workshop & Poster Abstracts Topic 17 - Systems biology and complex regulatory networks

17-1

The adaptive filter of the yeast galactose pathway.
Serge SMIDTAS, Vincent Schachter, Francois Kepes
BioInformatic, Genoscope, 2 rue Gaston Cremieu, Evry, 91000, France

17-2

Yeast chemogenomic approach to identify cellular pathways affected by the antifolates Methotrexate and Sulfanilamide.
Jose L. Revuelta, Javier Botet
Microbiologia y Genetica/IMB, Universidad de Salamanca/CSIC, C. M. Unamuno, Salamanca, 37007, Spain

17-3

Visualising the control of the respiratory clock in yeast.
Douglas Murray (1), Hiroaki Kitano (2)
(1) Kitano Symbiotic Systems Biology Project. Japan Science and Technology Agency. The Systems Biology Institute. 9S3 Shinanomachi Research Park. Keio School of Medicine. 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan; (2) Kitano Symbiotic Systems Project, 6-31-15 Jingumae, M-31 Suite 6A, Shibuya-ku, Tokyo 150-0001, Japan

17-4

The cycling of reserve carbohydrates in S. cerevesiae, a mathematical model.
Sven Kroppenstedt (1), Jacky L. Snoep (2), Florian F. Bauer (1)
(1) Institute for Winebiotechnolog, University of Stellenbosch, Private Bag XI, Matieland, 7602, South Africa; (2) Triple-J Group for Molecular Cell Physiologie, Dept. of Biochemistry, University of Stellenbosch, Private bag XI, Matieland 7602, South Africa

17-5

In vivo robustness/sensitivity analysis of cell cycle-related genes in S. cerevisiae.
Hisao Moriya, Hiroaki Kitano
JST Kitano Project, The Systems Biology Institute, 35 Shinano-machi, Shinjyuku-Ku, 160-8582, Japan

17-6

Remodeling of yeast genome expression under conditions of defective RNA polymerase III-dependent transciption.
Christine Conesa (1), Roberta Ruotolo (2), Pascal Soularue (3), Tiffany A. Simms (4), David Donze (4), André Sentenac (1), Giorgio Dieci (2)
(1) Service de Bioc. et Génét. Mol., CEA/Saclay, France; (2) Dep. of Bioc. and Biol. Mol., University of Parma, p.area Scienze 23/A, Parma, 43100, Italy; (3) Service de Génom. Fonct., CEA/Evry, France; (4) Dept. of Biol. Sciences, Baton Rouge, LA, USA

17-7

Contribution of metabolome studies for functional proteomics : the example of sulfur metabolism in yeast.
Alexandra Lafaye (1), Gilles Lagniel (2), Karin Vido (2), Christophe Junot (1), Eric Ezan (1), Jean Labarre (2)
(1) SPI/DRM CEA Saclay; (2) SBGM/DBJC, CEA Saclay, Centre de Saclay, Gif-Sur-Yvette, 91191, France

17-8

Two-dimensional transcriptome analysis in chemostat cultures: Combinatorial effects of oxygen availability and macronutrient limitation in Saccharomyces cerevisiae.
Siew Leng Tai (1), Viktor M. Boer (1), Pascale Daran-Lapujade (1), Michael C. Walsh (2), Johannes H. de Winde (3), Jean-Marc Daran (1), Jack T. Pronk (1)
(1) Department of Biotechnology, Delft University of Technology, Julianalaan 67, Delft, 2628 BC, The Netherlands; (2) Heineken Technical Services, Research and Development, Burgemeester Smeetsweg 1, 2380BB Zoeterwoude, The Netherlands; (3) DSM Life Sciences, Division of Bakery Ingredients, Technology Cluster, 2600MA Delft, The Netherlands

17-9

Early expression of yeast genes affected by chemical stress.
Vivienne Fardeau, Véronique Tanty, Mathilde Garcia, Claude Jacq, Fréderic Devaux
Biologie UMR8541, ENS, 46, rue d'Ulm, Paris, 75005, France

17-10

The synthetic genetic interaction spectrum of essential genes.
Armaity Davierwala (1), Jennifer Haynes (2), Zhijian Li (1), Renee Brost (1), Mark Robinson (1), Lisa Yu (3), Sanie Mnaimneh (1), Nicolle Preston (1), Hongwei Zhu (1), Yiqun Chen (1), Xin Cheng (1), Grant Brown (3), Charles Boone (1), Brenda Andrews (1), Tim Hughes (1)
(1) BBDMR, University of Toronto, 112 College St., Toronto, ON, M5G 1L6, Canada; (2) Department of Medical Genetics and Microbiology, University of Toronto; (3) Department of Biochemistry, University of Toronto

17-11

Model-driven analysis of transcriptional and metabolic networks in Saccharomyces cerevisiae.
Markus J. Herrgard, Qiang Hua, Byung-Kwan Cho, Vasiliy Portnoy, Baek-Seok Lee, Bernhard O. Palsson
Department of Bioengineering, UC San Diego, 9500 Gilman Dr, La Jolla, CA, 92093-0412, USA

17-12

Exploration of gene function and identification of kinase targets by systematic analysis of gene overexpression phenotypes.
Richelle Sopko (1), Dongqing Huang (1), Nicolle Preston (2), Gordon Chua (2), Huiming Ding (2), Kimberly Kafadar (3), Mike Snyder (4), Tim Hughes (2), Martha Cyert (3), Charlie Boone (2), Brenda Andrews (1)
(1) Department of Medical Genetics and Microbiology, University of Toronto, Toronto ON, Canada; (2) Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada; (3) Department of Biological Sciences, Stanford University, Stanford, California, USA; (4) Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, USA

17-13

Modeling of budding yeast G1 to S transition with Cln3/Far1 threshold control.
Riccardo L. Rossi, Vanoni Marco, Lilia Alberghina
Dip.Biotecnologie e Bioscienze, Università di Milano-Bicocca, P.za della Scienza 2, Milano, 20126, Italy

17-14

Systems-based analysis of Abf1 reveals coordination of mitotic growth and meiotic development via a highly dynamic protein-DNA interaction profile.
Ulrich Schlecht (1), Christa Niederhauser-Wiederkehr (1), Philippe Demougin (1), Nicolas Robine (2), Valérie Borde (2), Alain Nicolas (2), Michael Primig (1)
(1) Bioinformatics & Biochemistry, Biozentrum, University Basel, Klingelbergstr. 50, Basel, CH-4056, Switzerland; (2) CNRS UMR7147, Institut Curie, 25, rue d'Ulm, Pavillon Trouillet Rossignol, 75005 Paris, France

17-15

Mathematical modeling enlightens the dynamic response of yeast cells to hyperosmotic shock.

Workshop & Poster Abstracts Topic 18 - Other topics

18-1

Steryl ester hydrolases in yeast.
Andrea Wagner, Heidemarie Müllner, Guenter Deutsch, Guenther Daum
Institute of Biochemistry, Graz University of Technology, Petersgasse 12/2, Graz, 8010, Austria

18-2

Characterization of peroxisomes from the methylotrophic yeast Pichia pastoris.
Tamara Wriessnegger (1), Georg Guebitz (2), Erich Leitner (3), Elisabeth Ingolic (4), Guenther Daum (1)
(1) Institute of Biochemistry, Graz University of Technology, Petersgasse 12/II, Graz, 8010, Austria; (2) Institute of Environmental Biotechnology; Graz University of Technology, Austria; (3) Institute of Food Chemistry and Food Technology; Graz University of Technology, Austria; (4) Research Institute for Electron Microscopy; Graz University of Technology, Austria

18-3

Molecular and biochemical identification of a phenolic acid decarboxylase from Dekkera/Brettanomyces species.
Victoria Harris, Vladimir Jiranek, Paul Grbin
School of Agriculture and Wine, University of Adelaide, Waite Campus, PMB1, Glen Osmond, SA, 5064, Australia

18-4

Faf1p associates with proteins required for biogenesis of small subunits of ribosomes.
Bozenna Rempola, Iwona Karkusiewicz, Iga Piekarska, Joanna Rytka
Biophysics, IBB PAS, Pawinskiego 5A, Warsaw, 02-106, Poland

18-5

Ypi1, an inhibitory subunit of Glc7, is a phosphoprotein which interacts with Sds22.
Leda Pedelini (1), Pascual Sanz (2), Mª Adelaida García-Gimeno (2)
(1) Instituto de Biomedicina, Jaime Roig #11, 46010-Valencia, Spain; (2) Instituto de Biomedicina, CSIC, Jaime Roig #11, Valencia, 46010, Spain

18-6

PCR-mediated deletion of a defined chromosomal region in haploid Saccharomyces cerevisiae.
Minetaka Sugiyama (1), Toshimasa Nakazawa (1), Takahiro Sumiya (2), Atsushi Nakamura (2), Masafumi Nishizawa (3), Yoshinobu Kaneko (1), Satoshi Harashima (1)
(1) Dept. Biotech., Grad. Sch. Eng., Osaka Univ., 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan; (2) Infor. Media Center, Hiroshima Univ., 1-7-1 Kagami-yama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan; (3) Dept. Microbiol. Immunol., Keio Univ. Sch. Med., 35 Shinanomachi, Shinjuku, Tokyo, 160-8582, Japan

18-7

Drug susceptibilities, resistance mechanisms and their modulation in clinical yeast isolates.
Jana Cernicka (1), Zuzana Kozovska (1), Martina Hnatova (1), Monika Sojakova (2), Gyorgy Hajos (3), Julius Subik (1)
(1) Microbiology and Virology, Comenius University, Mlynska dolina B2, Bratislava, 842 15, Slovak Republic; (2) Comenius University in Bratislava, Faculty of Medicine, 1st Department of Obstetrics and Gynecology, Antolska 11, 851 07 Bratislava, Slovak Republic; (3) Hungarian Academy of Sciences, Chemical Research Center, Institute of Chemistry, Pusztaszery ut 59-67, 1025 Budapest, Hungary

18-8

CaNdt80 Involved in Drug Resistance by Regulating the Expression of CDR1 in Candida albicans.
Jang Shiun Wang (1), Chia-Geun Chen (1), Hsin-I Shih (2), Yun-Liang Yang (2), Hsiu-Jung Lo (1)
(1) Division of Clinical Research, National Health Research Institutes, Taiwan; (2) Department of Biological Science and Technology, National Chiao Tung University, Taiwan

18-9

Non-fatal Candida albicans cph1/cph1 efg1/efg1 transcription factor mutants forming filaments and causing inflammation in a mouse model of systemic infection.
Chia Geun Chen (1), Yun Liang Yang (2), Hsiao Hsu Cheng (1), Chia Li Su (1), Shiu Feng Huang (3), Chiung Tong Chen (4), Yu Tien Liu (5), Ih Jen Su (1), Hsiu Jung Lo (1)
(1) Division of Clinical Research, NHRI, 35 Keyan Road, Miaoli County, 350, Taiwan; (2) Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan; (3) Division of Molecular & Genomic Medicine, NHRI, Miaoli County; (4) Division of Biotechnology & Pharmaceutical Research, NHRI, Miaoli County, Taiwan; (5) Depertment of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan

18-10

Stable production of Aspergillus niger β-galactosidase by delta-mediated chromosomal integration in flocculent Saccharomyces cerevisiae.
Carla Oliveira, José Teixeira, Nelson Lima, Lucília Domingues
Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal

18-11

Characterization of strains between amylolytic yeast Schwanniomyces occidentalis and thermotolerant yeast Kluyveromyces marxianus constructed by protoplast fusion.
Vichai Leelavatcharamas (1), Masao Kishida (2), Haruhiko Kawasaki (2)
(1) Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand; (2) Life & Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka, 599-8531, Japan

18-12

Binding of the nucleolar protein Nep1 to a specific site in ribosomal RNA and investigation of Δ snr57 and multicopy RPS19 as nep1 suppressors.
Markus Buchhaupt, Peter Kötter, Karl-Dieter Entian
Institute of Microbiology, JWG University Frankfurt, Marie-Curie-Str. 9, Frankfurt am Main, 60439, Germany

18-13

Expression of plant β-glucosidase genes in Schizosaccharomyces pombe.
Bedia Palabiyik (1), Asim Esen (2), Güler Temizkan (1)
(1) The University of Istanbul, Faculty of Science, Department of Molecular Biology and Genetics, Vezneciler, Istanbul, 34118, Turkey; (2) Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA

18-14

New launch of Yeast Genetic Resource Center in Japan and its current activity.
Yoshinobu Kaneko (1), Aki Tada (1), Masayo Morita (2), Taro Nakamura (2), Satoshi Harashima (1), Chikashi Shimoda (2)
(1) Department of Biotechnology, Osaka University, Yamadaoka 2-1, Suita, 565-0871, Japan; (2) Department of Biology, Osaka City University, Sugimoto 3-3-138, Osaka 558-8585, Japan

18-15

Analysis of the complete mitochondrial genome of Nadsonia fulvescens var. elongata.
Ilona Pfeiffer (1), Judit Kucsera (2), Wladyslaw Golubev (3)
(1) Department of Microbiology, Faculty of Science, University of Szeged, Szeged, Hungary; (2) Microbiology, University of Szeged, Kozep fasor, Szeged, H-6726, Hungary; (3) Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia

18-16

Polymorphism in the mtDNA of the Cryptococcus neoformans species complex.
Judit Kucsera, Judit Litter, Nikolette Dobos, Andrea Keszthelyi, Zsuzsanna Hamari, Ilona Pfeiffer
Department of Microbiology, University of Szeged, Kozepfasor 52, Szeged, H-6726, Hungary

18-17

Genetic instability of a commercial Saccharomyces cerevisiae strain.
Dorit Schuller (1), Hugo Alves (1), Brigitte Gambon (2), Sylvie Dequin (2), Margarida Casal (1)
(1) Departamento de Biologia, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal; (2) Institut National de la Recherche Agronomique, UMR Sciences pour l'Oenologie, Place Viala, Montpellier, 34060, France

18-18

Studies on the use of the methylotrophic yeast Pichia pastoris for expressing the ORF3 protein of Hepatitis E virus.
Sunil Lal (1), Deepti Gupta (1), Lena Hammar (2), Holland Cheng (2)
(1) Virology Group, International Centre for Genetic Engg. & Biotechnology, New Delhi 110067, India; (2) Dept of Biosciences, Karolinska Institute, Huddinge, Sweden

18-19

Dimorphic yeast Yarrowia lipolytica shows phenotypic markers of apoptosis when exposed to hypoxia or treated with hydrogen peroxide.
Marek Mentel, Miroslava Baculíková, Gabriela Cellengová, Jordan Kolarov
Department of Biochemistry, Comenius University, Mlynska dolina CH-1, Bratislava, 842 15, Slovakia

18-20

Yeast Aconitase in Two Locations and Two Metabolic Pathways: Seeing Small Amounts is Believing.
Neta Regev-Rudzki, Sharon Karniely, Ophry Pines
Department Molecular Biology, Hebrew University of Jerusalem, Ein-Karem, Jerusalem, 91120, Israel