Effect of triclosan on a yeast strain dependent on a mitochondrial enoyl-ACP reductase of bacterial origin.
Alexander J. Kastaniotis, Fumi Okubo, J. Kalervo Hiltunen
Department of Biochemistry, University of Oulu, Linnanmaa campus, Oulu, 90570, Finland
Mitochondrial fatty acid synthesis of type II (FASII) is an evolutionarily conserved process in eukaryotes that is essential for respiration in Saccharomyces cerevisiae. The physiological role of this process, however, is not clearly understood. We have previously demonstrated that the respiratory deficient phenotype of a deletion of the ETR1 mitochondrial enoyl-thioester reductase gene can be at least partially complemented by the non-homologous fabI enoyl-ACP reductase gene from Escherichia coli if it is engineered to carry a mitochondrial localization sequence (mtFabI). The FabI protein is sensitive to the widely available drug triclosan. We have created a strain that has mtFabI integrated in the yeast genome, replacing the endogenous ETR1 gene. This strain is respiratory competent, but unlike the ETR1 wild type strain, it is sensitive to triclosan on media containing only a non-fermentable carbon source. On our poster, we will present work in progress on the effects of triclosan on the morphology of yeast cells grown into stationary phase.
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