The regulation of FAB1, a phosphatidylinositol 3-phosphate 5-kinase in Sacchromyces cerevisiae.
John P. Phelan, Frank T Cooke
Biochemistry and Mol Biology, UCL, Gower Street, London, WC1E 6BT, UK
Fab1 is a phosphatidylinositol 3-phosphate 5-kinase found in S.cerevisiae. It catalyses the conversion of phosphatidylinositol 3-phosphate to phosphatidylinositol 3,5 bisphosphate. When FAB1 is deleted from yeast, several phenotypes are observed: a swollen vacuole, inability to grow at 37C, defects in cell wall integrity, an acidified vacuole , loss of phosphatidylinositol 3,5 bisphosphate synthesis and misssorting of proteins at the multivesicular body. The aim of this study was to use the Yeast 2 Hybrid technique to identify effectors and regulators of Fab1 in an attempt to shed light on the mechanics behind the above phenotypes. Data will be presented on the results of this investigation.
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