Study of the relationship between yeast Htl1p and RSC complex.
Shan-Li Wang (1), Ali Tsai (2), Mingyuan Cheng (2)
(1) Institute of Genetics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan;
(2) Genetics, National Yang-Ming University, Shih-Pai, Taipei, 112, Taiwan
The chromatin remodeling RSC complex is a 1 Md complex comprising of at least 15 subunits. Recently, association of yeast Htl1p with RSC complex was established by identification of HTL1 interacting genes. Rsc8p was identified to interact with Htl1p by two-hybrid screening, in vitro binding assay and co-immunoprecipitation experiment. Further characterization of yeast cells deprived of Htl1p demonstrated that the 1 Md RSC complex is less stable especially at high temperature. When htl1 mutant was shifted to serine-deprived medium, like wild type cells, the transcription of CHA1 gene, known to be regulated by RSC complex, was repressed. However, persistant repression was observed only in htl1 mutant in serine-deprived medium. In addition, the chromatin profile of CHA1 promoter region was altered in htl mutant.
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