YGM 2004 Complete List of Abstracts

Abstract NumberSession TopicAbstract Title
1Epigenetic mechanismsHeterochromatin spreading at yeast telomeres occurs in M-phase.
Kristen Martins-Taylor, Scott Holmes
MB&B, Wesleyan University, Lawn Ave., Middletown, CT, 06459, USA
2Epigenetic mechanismsOn the formation of chromatin boundaries and the evolution of silencing.
Jasper Rine, Michael Kobor, Josh Barbiarz, Jennifer Gin, Bilge Ozaydin, Jessica Cande, Lenny Teytelman, Jason Zemansky
Molecular and Cell Biology, University of California, 522 Barker Hall, Berkeley, Ca, 94720-3202, USA
3Epigenetic mechanismsTranscriptional Silencing affects transcription at a step between activator binding and Pol II recruitment.
Lingyi Chen, Jonathan Widom
BMBCB, Northwestern University, 2153 Sheridan Road, Evanston, IL, 60208, USA
4ChromatinMcm10 plays a direct role in transcriptional silencing in Saccharomyces cerevisiae.
Ivan Liachko, Nancy L. Douglas, Bik K. Tye
Molecular Biology and Genetics, Cornell University, 327 Biotech Bldg, Ithaca, NY, 14853, USA
5ChromatinStructure and function of the Asf1 histone chaperone.
Carl Mann, Jean-Yves Thuret, Raphaël Guerois, Florence Mousson, Francoise Ochsenbein
SBGM and SBFM, CEA/Saclay, Bat. 144, Gif-sur-Yvette, 91191, France
6ChromatinGlucose repression and derepression mediated by H3 and H4 tails.
Juan Jose Infante, Rhiannon Biddick, Chris Tachibana, Elton T. Young
Biochemistry, University of Washington, Box 357350, Seattle, WA, 98195-7350, United States
7TranspositionMorphogenesis of the Ty3 viruslike particle.
Michael Aye (1), Pierre Baldi (2), Nadejda Beliakova-Bethell (1), Becky Irwin (1), Yimeng Dou (2), Min Zhang (1), Suzanne Sandmeyer (1)
(1) Biological Chemistry, University of California, University, Irvine, CA, 92697-1700, USA; (2) Information and Computer Science
8Protein sorting and turnoverGenome wide screen reveals a new role for acetylation in nuclear membrane targeting of proteins.
Athulaprabha Murthi, Anita. K. Hopper
Dept of Biochem & Mol Biol, Penn State University, 500 Univ Dr, Hershey, PA, 17033, U.S.A
9Protein sorting and turnoverCleanin' house: protein quality control in the nucleus.
Richard G. Gardner, Zara W. Nelson, Daniel E. Gottschling
Division of Basic Sciences, Fred Hutchinson CRC, 1100 Fairview Ave N., Seattle, WA, 98109, USA
10ReplicationCyclin B-Cdk activity stimulates meiotic re-replication in budding yeast.
Randy Strich (1), Michael Mallory (1), Michal Jarnik (1), Katrina Cooper (2)
(1) Cell & Developmental Biology, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA, 19111, USA; (2) Dept. of Biochemistry, Drexel College of Medicine, Philadelphia, PA
11RecombinationTid1 negatively regulates association of recombinase Dmc1 with chromatin when initiation of meiotic recombination is blocked.
Teresa M. Holzen (1), Parisha P. Shah (2), Heidi A. Olivares (3), Douglas K. Bishop (4)
(1) Committee on Genetics, University of Chicago, 920 E. 58th St., Chicago, IL, 60637, USA; (2) Department of Molecular Genetics and Cell Biology; (3) Department of Radiation and Cellular Oncology; (4) Department of Radiation and Cellular Oncology, Committee on Genetics, Dept. of Molecular Genetics and Cell Biology
12Mating/Sporulation/MeiosisSegregation of non-exchange chromosomes in the budding yeast Saccharomyces cerevisiae.
Rebecca Boumil (1), Benedict Kemp (2), Mara Stewart (3), Dean Dawson (3)
(1) Department of Molecular Biology, Massachusetts General Hospital Boston, MA; (2) Biology Department Dartmouth College Hanover, NH 03755; (3) Molecular Microbiology, Tufts University, 136 Harrison Ave., Boston, MA, 02111, USA
13CentromeresThe role of the centromeric histone variant in kinetochore specification and assembly.
Kimberly A. Collins, Sue Biggins
Basic Sciences, FHCRC, 1100 Fairview Ave. N, Seattle, WA, 98109, USA
14CentromeresSuppressor analysis of histone mutants defective in chromosome segregation.
Hasna Kanta, Marsha Rhoads, Inés Pinto
Biological Sciences, University of Arkansas, SCEN 601, Fayetteville, AR, 72701, USA
15Chromosome structureBudding yeast Pds5p regulates sister chromatid cohesion maintenance and is sumoylated to promote cohesion dissolution.
Cristina Aguilar (1), Kristen Stead (1), Christian Davidson (1), Ke Zheng (1), Gary Fortin (1), Pamela Meluh (2), Vincent Guacci (1)
(1) Basic Science, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA; (2) Memorial Sloan-Kettering, Program in Molecular Biology, New York, NY 10021
16Nucleo/cytoplasmic transportStress-induced oscillatory shuttling of Msn2 and Msn4 in and out the nucleus.
Cecilia Garmendia (1), Georges Renault (1), Sylvie Lallet (1), Albert Goldbeter (2), Michel Jacquet (1)
(1) IGM, Université Paris-Sud, bat 400, Orsay, 91405, France; (2) Unité de Chronobiologie théorique, Faculté des Sciences, Université Libre de Bruxelles, B-1050 Brussels, Belgium
17TranscriptionThe Nup84 nuclear pore subcomplex mediates segregation of the opposing Rap1 roles in transcriptional repression and activation.
Balaraj Menon (1), Nayan Sarma (1), Satish Pasula (1), Stephen Deminoff (2), Kristine Willis (3), Kellie Barbara (1), Brenda Andrews (3), George Santangelo (3)
(1) Department of Biological Sciences, University of Southern Mississippi, 118 College Drive, Hattiesburg, MS 39406; (2) Department of Molecular Genetics, The Ohio State University, 484 W. 12th Ave., Columbus, Ohio 43210; (3) Medical Genetics, University of Toronto, 1 Kings College Cir., Toronto, ON, M5S 1A8, Canada
18TranscriptionAmino acid substitutions in the switch 2 region of the Rpb1 subunit of S. cerevisiae RNAPII confer downstream shifts in transcription start site selection.
Robert C. Majovski, Alfred S. Ponticelli
Biochemistry, University at Buffalo, 3435 Main St., Buffalo, NY, 14214, United States
19TranscriptionGal80's linked interactions: a new model for the GAL gene switch.
Vepkhia Pilauri, Cuong Diep, James Hopper
Biochem & Mol Biol, Penn State Univ, 500 University Drive, Hershey, PA, 17033, USA
20TranscriptionThe role of Swi/Snf at the HO promoter: new insights.
Doyel Mitra, David Stillman
Department of Pathology, University of Utah, 30 North 1900 East, Salt Lake City, UT, 84132, U.S.A.
21GenomicsGrowth-dependent gene expression in Saccharomyces cerevisiae.
Birgitte Regenberg (1), Thomas Grotkjær (2), Ole Winther (3), Christoffer Bro (2), Jan von Köller (2), Anders Fausbøll (4), Mats Åkesson (5), Lars Kai Hansen (3), Jens Nielsen (2)
(1) Biozentrum, J. W. Goethe-Universität, Marie-Curie-Str. 9, Frankfurt am Main, 60439, Germany; (2) Center for Microbial Biotechnology, Building 223 Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; (3) Informatics and Mathematical Modelling, Building 321 Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; (4) Center for Biological Sequence Analysis, Building 208 Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; (5) Novo Nordisk A/S, BioProcess Laboratories, Novo Allé, DK-2880 Bagsværd, Denmark
22Signal transductionComprehensive genome-wide characterization of the pheromone response.
Corey Nislow (1), Angela M. Chu (1), Fred Naider (2), Ronald W. Davis (1), Guri Giaever (1)
(1) Department of Biochemistry, Stanford Genome Tech Center, 855 California Ave., Palo Alto, CA, 94304, United States; (2) Department of Chemistry, College of Staten Island and Institute for Macromolecular Assemblies, City University of New York, Staten Island, New York 10314
23Signal transductionDifferential regulation of a Tec1-Ste12 transcription factor complex during invasive growth and mating in Saccharomyces cerevisiae.
Song Chou, Haoping Liu
Biological Chemistry, Univ. of California, Irvine, 19182 Jamboree Rd., Irvine, CA, 92697, U.S.A.
24Signal transductionHow the Fus3 MAP kinase ensures signaling specificity during mating and filamentous growth in S. cerevisiae.
Marie Bao, Monica Schwartz, Hiten Madhani
Biochemistry and Biophysics, UCSF, 600 16th St. N374, San Francisco, CA, 94143, USA
25Signal transductionMaintenance of MAPK signaling specificity: Origins of cross-talk between the HOG pathway and the mating pheromone and filamentous growth pathways..
Patrick J. Westfall, Jeremy Thorner
Biochemistry and Molecular Bio, UC Berkeley, 528 Barker Hall, Berkeley, CA, 94720, USA
26Signal transductionThe signaling mucin Msb2 interacts with the HOG pathway osmosensor Sho1 and polarity establishment GTPase Cdc42 to promote MAPK-dependent activation of the filamentous growth pathway.
Paul J. Cullen (1), Ellie Graham (2), Walid Sabbagh (3), Lee Bardwell (3), George F. Sprague, Jr. (2)
(1) Institute of Molecular Biology, University of Oregon, Eugene OR 97403, Address starting September 1st: Department of Biological Sciences, SUNY at Buffalo, Buffalo, NY 14260; (2) Institute of Molecular Biology, University of Oregon, Eugene OR 97403; (3) Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697
27Signal transductionTurgor restoration : the critical event in signal cessation of the HOG pathway.
Bodil Nordlander (1), Peter Gennemark (2), Markus J Tamás (1), Sara Karlgren (1), Dag Wedelin (2), Stefan Hohmann (1)
(1) Cell and Molecular Biology, Göteborg University, Box 462, Göteborg, S-40530, Sweden; (2) Computing Science, Chalmers University of Technology, Rännvägen 6B, 41296 Göteborg, Sweden
28GenomicsIdentification of transcription factor targets by activation-based strategies.
Gordon Chua (1), Richelle Sopko (2), Jeff Pootoolal (1), Owen Ryan (1), Armaity Davierwala (1), Stuart Yang (1), Quaid Morris (1), Brenda Andrews (2), Charlie Boone (1), Tim Hughes (1)
(1) B. and B. Medical Genetics, University of Toronto, 112 College Street, Toronto, ON, M5G 1L6, Canada; (2) Department of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Medical Sciences Building, Rm 4287, Toronto, Ontario, M5S 1A8, Canada.
29GenomicsQuiescent yeast cells exhibit a rapid response to oxidative stress.
Anthony D. Aragon, Gabriel A. Quinones, Margaret Werner-Washburne
Biology, University of New Mexico, Castetter Hall, Albuquerque, NM, 87131, USA
30ProteomicsGlobal proteomic & metabolic profiling of yeast cells.
Edward Marcotte, Peng Lu, John Prince, Anu Rangan, Sherwin Chan, Dean Appling, David Hoffman
Inst. for Cell & Molec Biology, University of Texas at Austin, 2500 Speedway, Austin, TX, 78712, USA
31ProteomicsProteomic analysis of eukaryotic translation complexes.
Tracey C. Fleischer, Connie M. Weaver, Jennifer L. Jennings, Andrew J. Link
Microbiology & Immunology, Vanderbilt School of Medicine, 1161 21st Ave South, Nashville, TN, 37232, USA
32Cell Biology: OtherGenetic and proteomic analysis of ribosome assembly.
John Woolford, Piyanun Harnpicharnchai, Tiffany Miles, Edward Horsey, Jelena Jakovljevic, Lan Tang
Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh,PA, PA, 15213, USA
33ProteomicsIdentification of yeast kinase substrates using protein chips.
Jason Ptacek (1), Geeta Devgan (2), Heng Zhu (2), Xiaowei Zhu (3), Greg Michaud (4), Barry Schweitzer (4), Paul Predki (4), Michael Snyder (2)
(1) Mol. Biophysics and Biochem, Yale University, P.O. Box 208103, New Haven, CT, 06520, USA; (2) Department of Molecular, Cellular and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520; (3) Department of Compuational Biology and Bioinformatics, Yale University, P.O. Box 208103, New Haven, CT 06520; (4) Protometrix, Inc., 688 East Main St., Branford, CT 06405
34Global Analysis: OtherGenome stability determinants in viable yeast: old new and surprises.
Forrest A Spencer (1), Cheryl D Warren (1), Karen Yuen (2), Ou Chen (1), Philip A Hieter (2)
(1) Institute of Genetic Medicine, Johns Hopkins University, 720 Rutland Ave, Baltimore, MD, 21205, USA; (2) CMMT, Univ of British Columbia, 950 W 28th Ave, Rm 2026, Vancouver, BC V5Z 4H4 CANADA
35Global Analysis: OtherIntergenic regions, ARS elements, and transcription: a role for molecular shielding.
Yves Sucaet (1), Christi Magrath (2)
(1) Troy State University, Biological and Environmental Science, Troy, AL 36082; (2) Biological & Environmental, Troy State University, University Blvd., Troy, AL, 36082, United States of America
36Chromosome structureMolecular origin and fate of segmental duplications in the Saccharomyces cerevisiae genome.
Romain Koszul, Bernard Dujon, Gilles Fischer
Génétique Mol. des Levures, Institut Pasteur, 25 rue du Dr Roux, PARIS, 75724, FRANCE
37Other yeastsCytoplasmic dynein couples mitosis and morphogenesis in Candida albicans.
Kenneth Finley, Judith Berman
Genetics, Cell Biology & Dev., University of Minnesota, 321 Church St SE, Minneapolis, MN, 55455, USA
38Metabolism/membrane traffickingPolarization of plasma membrane lipids contributes to hyphal morphogenesis in Candida albicans.
Stephen Martin, Javier Alvarez, James Konopka
Molecular Genetics & Microbiol, SUNY Stony Brook, Life Sciences Bldg., Stony Brook, NY, 11794-5222, USA
39CytoskeletonThe asymmetry of proteins at the neck of budding yeast is dependent on the proper assembly of septins.
Lukasz Kozubowski, Jennifer Larson, Kelly Tatchell
Biochemistry and Mol. Biol., LSU Health Sciences Center, 1501 Kings Hwy., Shreveport, LA, 71130, USA
40CytoskeletonA genetic dissection of Aip1p identified sites of interactions with actin and cofilin.
Michael G. Clark, Brian Haarer, David C. Amberg
Dept. of Biochem. & Mol. Bio., SUNY Upstate Medical Univ., 750 East Adams St., Syracuse, NY, 13210, USA
41Cell cycle/Growth control/MetabolismDynactin complex is involved in a novel checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae.
Yoshikazu Ohya, Masaya Suzuki, Ryoji Igarashi, Takahiko Utsugi, Masashi Yukawa
Grad. Sch. of Frontier Science, University of Tokyo, Bldg. FSB-101, 5-1-5, Kashiwa, 277-8562, Japan
42Protein sorting and turnoverThe yapsins are a family of GPI-linked aspartyl proteases required for cell wall integrity in Saccharomyces cerevisiae.
Damian Krysan (1), Paula Magnelli (2), Claudia Abeijon (2), Robert Fuller (3)
(1) Pediatric Infectious Disease, University of Michigan, 1500 E. Medical Center Dr. Ann Arbor MI 48109-0244; (2) Molecular and Cell Biology, Boston University School of Dental Medicine, 700 Albany St. Boston MA 02118; (3) Bilogical Chemistry, University of Michigan, 1301 E. Catherine St., Ann Arbor MI 48109
43RNA turnoverRibosome stalling at an upstream open reading frame regulates nonsense-mediated mRNA decay.
Anthony Gaba (1), Allan Jacobson (2), Matthew S. Sachs (1)
(1) Environ. Biomolec. Systems, Oregon Health & Science Univ, 20000 NW Walker Road, Beaverton, OR, 97006, US; (2) Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655-0122
44RNA processingRNA 3' end Processing by the Nuclear Exosome Controls NAB2 mRNA Levels.
Kelly Roth, Maria Wolff, Marie Rossi, Scott Butler
Microbiology and Immunology, University of Rochester, 601 Elmwood Ave., Rochester, NY, 14642, USA
45RNA turnoverPolyadenylation and degradation of hypomodified tRNAiMet.
Sujatha Kadaba (1), Annette Krecic (1), Tamyra Trice (1), Anna Krueger (2), Alan Hinnebusch (3), James Anderson (1)
(1) Biological Sciences, Marquette University, 530 n 15th St, Milwaukee, WI, 53233, United States; (2) Department of Biology, Johns Hopkins University, Baltimore, MD 21218 USA; (3) Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892 USA
46TranscriptionMaf1 and the integration of signaling pathways mediating transcriptional repression by RNA polymerase III.
JaeHoon Lee, Neelam Desai, Rajendra Upadhya, Ian Willis
Department of Biochemistry, Albert Einstein Col. Medicine, 1300 Morris Park Ave, Bronx, NY, 10461, USA
47Signal transductionActivation of eIF2alpha kinase GCN2 requires GCN1-ribosome interaction and attendant modulation of A-site function by GCN1.
Evelyn Sattlegger, Alan G Hinnebusch
National Institutes of Health, NICHD, 9000 Rockville Pike, Bethesda, MD, 20892-2427, USA
48Epigenetic mechanismsThe NatA Na-acetyltransferase complex modulates conversion of Sup35 to the prion ([PSI+]) state.
Rochele R. Yamamoto, Tricia R. Serio
MCB, Brown University, 69 Brown Street, Providence, RI, 02912, USA
49Evolution/Comparative genomicsSynteny patterns: A message from the past for today's interpretation of Yeast genomes.
Sophie Bracht (1), Fred Dietrich (2), Sylvia Voegeli (1), Anita Lerch (1), Riccarda Rischatsch (1), Iza Kaminski (1), Peter Philippsen (1)
(1) Applied Microbiology, Biozentrum, University Basel, Klingelbergstr. 50, Basel, 4056, Switzerland; (2) Department of Molecular Genetics and Microbiology, Duke University, USA
50Evolution/Comparative genomicsGenome Evolution in the Hemiascomycete Yeasts.
The Génolevures 2 Sequencing Consortium (1), Jean-Luc Souciet (2)
(1) Génoscope (Evry), INA-PG (Thiverval-Grignon), Institut Pasteur (Paris), University of Paris XI (Orsay), University of Bordeaux 2, University of Lyon 1, University of Strasbourg 1.; (2) FRE 2326 ULP/CNRS, Institut de Botanique, 28 rue Goethe, Strasbourg, F-67083, FRANCE
51Evolution/Comparative genomicsExploring Genome Plasticity and Adaptive Evolution in de novo Hybrid Yeast Species.
Barbara Dunn (1), Gianni Liti (2), Edward J. Louis (2), Frank Rosenzweig (3), Gavin Sherlock (1)
(1) Dept. of Genetics, Stanford University Med. Sch., 300 Pasteur Ave, Stanford, CA, 94305-5120, USA; (2) Dept. of Genetics, University of Leicester, LE1 7RH Leicester UK; (3) Divn. of Biological Sciences, Univ. of Montana, Missoula, MT 59812
52Mutagenesis/RepairA distinctive mutation spectrum associated with transcription in Saccharomyces cerevisiae.
Malcolm J. Lippert (1), Jennifer A. Freedman (2), Melissa A. Barber (1), Sue Jinks-Robertson (2)
(1) Biology Department, Saint Michael's College, 1 Winooski Park, Colchester, VT, 05439, USA; (2) Biology Department, Emory University, 1510 Clifton Rd., Atlanta, GA 30322 USA
53Mutagenesis/RepairTowards understanding the mechanisms of palindromic gene amplification.
Alison Rattray, Anne Welcker, Brenda Shafer, Jeffrey Strathern
GRCBL, NCI-FCRDC, PO Box B, Frederick, MD, 21702, USA
54Mutagenesis/RepairA biochemical characterization of the MSH2-MSH6 and MLH1-PMS complexes of Saccharomyces cerevisiae.
Marc Mendillo, Dan Mazur, Richard Kolodner
Ludwig Inst For Cancer Res., UC San Diego, MC 0669, La Jolla, CA, 92093, USA
55RecombinationMechanism of illegitimate recombination in Kluyveromyces lactis.
Andreas Kegel, Sidney Carter, Stefan Äström
Developmental Biology, Stockholm University, Svante Arrheniusv 17, Stockholm, 10691, Sweden
56TranspositionTy1 mobilizes subtelomeric Y´ elements in telomerase-negative S. cerevisiae survivors.
Patrick H. Maxwell (1), Candice Coombes (2), Alison E. Kenny (1), Joseph L. Lawler (2), Jef D. Boeke (2), M. Joan Curcio (1)
(1) Developmental Genetics, Wadsworth Center, 150 New Scotland Ave, Albany, NY, 12208, USA; (2) Dept of Molecular Biology & Genetics, The Johns Hopkins University School of Medicine, 617 Hunterian Bldg, 725 N. Wolfe St., Baltimore, MD 21205
57TranspositionTy1 copy number oscillation in Saccharomyces.
David J. Garfinkel (1), Sharon P. Moore (1), Gianni Liti (2), Karen M. Stefanisco (1), Katherine M. Nyswaner (1), Caroline Chang (1), Edward J. Louis (2)
(1) National Cancer Institute, National Institutes of Health, PO Box B, Frederick, MD, 21702-1201, USA; (2) Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK
58Protein sorting and turnoverVisualizing cisternal maturation in living yeast.
Catherine A. Reinke, Brooke J. Bevis, Eugene Losev, Daniel E. Strongin, Benjamin S. Glick
Mol. Gen. and Cell Bio., University of Chciago, 920 E. 58th St., Chicago, IL, 60637, USA
59Metabolism/membrane traffickingIntact ArfGAP function is required for the generation of COPI vesicles.
Stephen Lewis (1), Pak Phi Poon (1), Richard Singer (2), Gerald Johnston (1), Anne Spang (3)
(1) Microbiology & Immunology, Dalhousie University, 5850 College Street, Halifax, NS, B3H 1X5, Canada; (2) Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, NS B3H 1X5, Canada; (3) Friedrich Miescher Laboratorium, Max-Planck-Gesellschaft, Spemannstrasse 39 D-72076, Tubingen, Germany
60Protein sorting and turnoverDistinct machinery is required for the ER-associated degradation of a multispanning membrane protein and a soluble lumenal protein.
Gregory Huyer (1), Wachirapon F. Piluek (1), Jeffrey L. Brodsky (2), Susan Michaelis (1)
(1) Cell Biology, Johns Hopkins Medical School, 725 N. Wolfe Street, Baltimore, MD, 21205, USA; (2) Dept. of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260
61Cell Biology: OtherImmunity to yeast killer toxin K28: interaction of retrograde internalised toxin with the preprotoxin in the cytosol.
Frank Breinig, Tanja Sendzik, Manfred Schmitt
Applied Molecular Biology, Saarland University, Building 2, Saarbruecken, 66123, Germany
62Protein sorting and turnoverA role for ubiquitin in the cytosol to vacuole trafficking pathway.
Bonnie Baxter (1), Hagai Abeliovich (2), David Goldfarb (1)
(1) Department of Biology, University of Rochester, 435 Hutchison Hall, Rochester, NY, 14627, USA; (2) Hebrew University of Jerusalem, Faculty of Agriculture, P.O.B. 12, Rehovot, 76100 Israel
63Mitochrondria/Vacuoles/PeroxisomesPhosphoinositide- and GTP hydrolysis-dependent segregation of two AAA family ATPases from ergosterol- and ceramide-rich membrane domains is required for membrane fusion.
Tatiana Boukh-Viner, Tong Guo, Robert Kyskan, Svetlana Milijevic, Christopher Gregg, Andre Cerracchio, Sandra Haile, Alex Alexandrian, Vivianne Wong, Jonathan Solomon, Vladimir Titorenko
Department of Biology, Concordia University, 7141 Sherbrooke Str., Montreal, PQ, H4B 1R6, Canada
64Metabolism/membrane traffickingPhospholipid metabolism regulated by a transcription factor sensing phosphatidic acid on the endoplasmic reticulum.
Christopher Loewen (1), Laura Gaspar (2), Steve Jesch (2), Christine Delon (3), Nicholas Ktistakis (3), Susan Henry (2), Timothy Levine (4)
(1) Division of Cell Biology, Institute of Ophthalmology, UCL, Bath Street, London EC1V 9EL, UK; (2) Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA; (3) Signalling Programme, Babraham Institute, Babraham, Cambridge CB2 4AT, UK; (4) Cell Biology, Institute of Ophthalmology UCL, 11-43 Bath Street, london, EC1V 9EL, UK
65Metabolism/membrane traffickingOsmotic shock induced regulation of the signaling lipid PI3,5P2.
Jason E. Duex, Johnathan J. Nau, Lois S. Weisman
Biochemistry, University of Iowa, 2117 MERF, Iowa City, IA, 52246, United States
66Protein sorting and turnoverEssential role for phosphoinositide phosphatases in the regulation of PI(3)P in yeast.
William Parrish, Christopher Stefan, Scott Emr
Cellular and Molecular Medicin, UCSD, 9500 Gilman Dr., San Diego, CA, 92093-0668, USA
67Cell cycle/Growth control/MetabolismCln3 activates G1-specific transcription via phosphorylation of the SBF-bound repressor, Whi5.
Robertus de Bruin (1), W. Hayes McDonald (2), Tatyana Kalashnikova (1), John R. Yates, III (2), Curt Wittenberg (3)
(1) Molecular Biology, The Scripps Research Institute, 10550 N. Torrey Pine, La Jolla, CA, 92037, USA; (2) Dept. Cell Biology; (3) Depts. of Molecular Biology and Cell Biology, MB3, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA
68TranscriptionA Reb1p-CLB2 UAS complex is modulated by an activity associated with glutaminyl tRNA synthetase that is likely a protease.
Lakmal Kotelawala, Jodi Reynolds, Eric Phizicky, Elizabeth J. Grayhack
Biochemistry & Biophysics, University of Rochester, 601 Elmwood Avenue, Rochester, NY, 14642, USA
69TranscriptionThe Paf1 complex: associated with RNA polymerase II and important for post-transcriptional processes.
Kristi Penheither (1), Cherie Mueller (1), Stephanie Porter (1), Judith Jaehning (2)
(1) Molecular Biology Program, University of Colorado HSC, 4200 E. 9th Ave., Denver, CO, 80262, USA; (2) Department of Biochemistry and Molecular Genetics, UCHSC
70TranscriptionA functional requirement for the Saccharomyces cerevisiae Paf1 complex in RNA 3' end formation.
Kathryn E. Kumer, David M. Mauger, Karen M. Arndt
Biological Sciences, University of Pittsburgh, 4259 Fifth Ave., Pittsburgh, PA, 15260, U.S.A.
71TranscriptionSen1 helicase is a transcription termination factor for RNA polymerase II.
Eric Steinmetz, David Brow
Biomolecular Chemistry, University of Wisconsin, 1300 University Ave, Madison, WI, 53706, USA
72TranscriptionSsu72, a component of the CPF 3'-processing machinery in yeast, is an RNAPII CTD phosphatase with specificity for serine-5.
Krishnamurthy Shankarling (1), Xiaoyuan He (2), Mariela Reyes-Reyes (1), Claire Moore (2), Michael Hampsey (1)
(1) Department of Biochemistry, RWJ Medical School, UMDNJ, 683, Hoes Lane West, Piscataway, NJ, 08854, USA; (2) Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111
73Informatics/Computational biologyA network analysis of synthetic lethal genetic interaction.
Sharyl L. Wong (1), Lan V. Zhang (1), Gabriel F. Berriz (1), Debra S. Goldberg (1), Oliver D. King (1), Amy H. Y. Tong (2), Zhijian Li (2), Guillaume Lesage (3), Brenda Andrews (2), Howard Bussey (3), Marc Vidal (4), Charles Boone (2), Frederick P. Roth (1)
(1) Biol Chem & Molec Pharm. Dept., Harvard Medical School, 250 Longwood Ave, Boston, MA, 02115, USA; (2) Banting and Best Dept. of Medical Research and Dept. of Medical Genetics and Microbiology, University of Toronto, Canada.; (3) Dept. of Biology, McGill University, Montreal PQ, Canada.; (4) Dept. of Cancer Biology, Dana-Farber Cancer Institute and Dept. of Genetics, Harvard Medical School, Boston, MA, USA.
74Cell cycle/Growth control/MetabolismNetwork analysis of the cell cycle response of Saccharomyces cerevisiae to linoleic acid hydroperoxide.
Mark D. Temple, Nazif Alic, Moritz Durchdewald, Chii S. Fong, Ian W. Dawes
Ramaciotti Ctr Gene Func Anal, School of Biotech & Biomol Sci, University of NSW, Sydney, NSW 2052, Australia.
75Informatics/Computational biologyComprehensive analysis of gene regulatory pathways and mechanistic model building through an integrated computational platform for Saccharomyces cerevisiae.
Xufei Qian (1), Shubhada Godbole (2), Michael Baitaluk (1), Erdem Kurul (1), Amarnath Gupta (1), Animesh Ray (2)
(1) San Diego Supercomputer Center, University of California San Diego, La Jolla, California; (2) Systems Biology, Keck Graduate Institute, 535 Watson Drive, Claremont, CA, 91711, USA
76
Presentation Cancelled
77Informatics/Computational biologyGenome-scale reconstruction of Saccharomyces cerevisiae.
Natalie Duarte, Markus Herrgard, Bernhard Palsson
Bioengineering, Univ of California, San Diego, 9500 Gilman Dr, La Jolla, CA, 92037-0412, USA
78Signal transductionMating by the numbers: quantitative measurements and computational modeling of the pheromone response.
Kirsten R. Benjamin (1), Larry Lok (1), Ty Thomson (2), Drew Endy (2), Roger Brent (1)
(1) Alpha Project, Molecular Sciences Institute, 2168 Shattuck Ave., Berkeley, CA, 94704, USA; (2) Division of Biological Engineering & Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139
79Cell cycle/Growth control/MetabolismSir2-independent life span extension by calorie restriction or SCH9 deletion.
Matt Kaeberlein (1), Kathryn Kirkland (2), Stanley Fields (1), Brian Kennedy (2)
(1) Department of Genome Sciences, University of Washington, HSB K-222, Seattle, WA, 98195, USA; (2) Department of Biochemistry, University of Washington, Seattle WA 98195
80Cell cycle/Growth control/MetabolismPhysiological state of the glucose-limited chemostat corresponds to early diauxic shift.
Matthew J. Brauer, Alok J. Saldanha, David Botstein
Inst. for Integrative Genomics, Princeton University, Washington Rd., Princeton, NJ, 08544, USA
81Signal transductionThe Schizosaccharomyces pombe G alpha protein Gpa2 regulates glucose-induced cAMP production through a direct interaction with adenylate cyclase.
F. Douglas Ivey, Charles S. Hoffman
Biology Department, Boston College, 140 Commonwealth Ave, Chestnut Hill, MA, 02467, USA
82Signal transductionPak1 protein kinase regulates both activation and nuclear localization of Snf1-Gal83 protein kinase.
Kristina Hedbacker, Seung-Pyo Hong, Marian Carlson
Genetics and Development, Columbia University, 701 W 168th St, New York, NY, 10032, USA
83Signal transductionTap42 and Tip41, two PP2A-interacting proteins, act in concert to regulate a subset of rapamycin-induced genes.
Katrin Düvel, James Broach
Dept. of Molecular Biology, Princeton University, Washington Road, Princeton, NJ, 08544, USA
84Cell cycle/Growth control/MetabolismGetting started: a role for RPB4 in the growth response to fresh medium.
Warren Heideman, Matthew Slattery, Dominic Porcaro, Dritan Liko
Pharmaceutical Sciences, University of Wisconsin, 777 Highland Ave, Madison, WI, 53705, United States
85ACytoskeletonCharacterizing the role of the actin-interacting MAPKKK Ssk2p in the recovery of the actin cytoskeleton after osmotic stress.
Blaine T. Bettinger, Tatiana Yuzyuk, David C Amberg
Biochemistry & Molecular Bio, SUNY Upstate Medical Univ., 750 E. Adams St., Syracuse, NY, 13207, USA
86BCytoskeletonGenetic interactions with RVS161 and RVS167.
Helena Friesen, Christine Humphries, Oliver Schub, Brenda Andrews
Dept. of Medical Genetics, University of Toronto, 1 King's College Cir, Toronto, M5S 1A8, Canada
87CCytoskeletonIn vitro and in vivo analyses of mutations that alter the target-binding of Abp1 SH3 domains.
Jennifer Haynes (1), Bianca Garcia (2), Arianna Rath (2), Alan Davidson (2), Brenda Andrews (1)
(1) Molecular and Medical Genetics, University of Toronto, 1 King's College Cir, Toronto, ON, M5S 1A8, CANADA; (2) Department of Biochemistry, University of Toronto
88ACytoskeletonFrom function to shape - the role of a Formin in tip-branching and hyphal formation in Ashbya gossypii.
Andreas Kaufmann, Michael Koehli, Pierre Philippe Laissue, Peter Philippsen, Hans-Peter Schmitz
Applied Microbiology, Biozentrum University of Basel, Klingelbergstrasse 5, Basel, 4056, Switzerland
89BCytoskeletonAdvances in the use of FRET for protein structure determination and its application to the study of the spindle pole body.
Eric Muller, Brian Snydsman, Bryan Sundin, Bethany Fox, Dale Hailey, Trisha Davis
Dept. Biochemistry, Box 357350, University of Washington, 1959 NE Pacific St., Seattle, WA, 98195, US
90CCytoskeletonTwo novel sub-domains within the cargo-binding domain of myosin V.
Natasha Pashkova (1), Natalie L. Catlett (1), Jennifer L. Novak (1), Robert E. Cohen (1), Guanming Wu (2), Renne Lu (2), Lois S. Weisman (1)
(1) Department of Biochemistry, University of Iowa, 51 Newton Road, Iowa City, IA, 52242, USA; (2) Boston Biomedical Research Institute, Watertown, MA, 02472, USA
91ACytoskeletonThe regulation of FAB1, a phosphatidylinositol 3-phosphate 5-kinase in Sacchromyces cerevisiae.
Phelan John P, Frank T Cooke
Biochemistry and Mol Biology, UCL, Gower Street, London, WC1E 6BT, UK
92BCytoskeletonRole of Nucleotide binding in Yeast Septins.
Ashok Rajendran (1), Satish Nagaraj (1), Alina Vrabioiu (2), Christine Field (2), Mark longtine (1)
(1) Biochemistry & Molecular Biol., Oklahoma State University, 246 NRC, Stillwater, OK, 74078, United States of America; (2) Department of Cell Biology, Harvard Medical School, #107, Louis Pasteur Avenue, Boston, MA 02115.
93CCytoskeletonProtein-protein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae.
Matthias Versele, Björn Gullbrand, Raymond Chen, Jeremy Thorner
Dept. of Molec. & Cell Biology, Univ. of California, Berkeley, Rm. 16, Barker Hall, Berkeley, CA, 94720-3202, USA
94AMitochrondria/Vacuoles/PeroxisomesMutagenesis screen of Saccharomyces cerevisiae Cox11p.
Graham Banting, D. Moira Glerum
Medical Genetics, University of Alberta, 833MedSci, Edmonton, AB, T6G 2H7, Canada
95BMitochrondria/Vacuoles/PeroxisomesSUMO Modification of Mitochondrial Protein Mgm101p.
Leah Jablonski, Anat Kohn, Elaine Sia
Biology, University of Rochester, 334 Hutchison Hall, Rochester, NY, 14127, USA
96C
Poster Withdrawn/Cancelled
97AMitochrondria/Vacuoles/PeroxisomesEffect of triclosan on a yeast strain dependent on a mitochondrial enoyl-ACP reductase of bacterial origin.
Alexander J. Kastaniotis, Fumi Okubo, J. Kalervo Hiltunen
Department of Biochemistry, University of Oulu, Linnanmaa campus, Oulu, 90570, Finland
98BMitochrondria/Vacuoles/PeroxisomesSynthetic lethality to elucidate cardiolipin function.
Guiling Li, Miriam Greenberg
BIOLOGICAL SCIENCES, WAYNE STATE UNIVERSITY, 5047 GULLEN MALL, DETROIT, MI, 48202, UNITED STATES
99CMitochrondria/Vacuoles/PeroxisomesA signaling pathway between mitochondria and vacuole affects iron and copper metabolism.
Liangtao Li, Jerry Kaplan
Pathology, University of Utah, 50 No. Medical Drive, Salt Lake City, UT, 84132, U.S.A

100AMitochrondria/Vacuoles/PeroxisomesThe existence in Saccharomyces cerevisiae of a protective mechanism in response to methylglyoxal: the role of D-lactate movement across the inner mitochondrial membrane and its function on gluconeogenesis.
Maria Luigia Pallotta
S.A.V.A, University of Molise, Via De Sanctis, Campobasso, 86100, Italy
101BMitochrondria/Vacuoles/PeroxisomesParticipation of Msh1p in mitochondrial genome stability: repair, recombination, and morphology.
Shona A. Mookerjee, Elaine A. Sia
Department of Biology, University of Rochester, 334 Hutchison Hall, Rochester, NY, 14627, USA
102CMitochrondria/Vacuoles/PeroxisomesComplementation of S. cerevisiae COX6 null mutations with mammalian homologs.
James J. O'Donnell III, Stephanie C. Schroeder
Biological Sciences, Webster University, 470 E. Lockwood Ave, St Louis, MO, 63119, USA
103AMitochrondria/Vacuoles/PeroxisomesA search for proteins involved in mitochondrial genome replication and maintenance.
NAINA PHADNIS, ELAINE SIA
BIOLOGY, UNIVERSITY OF ROCHESTER, 334 HUTCHISON HALL, Rochester, NY, 14620, USA
104BMitochrondria/Vacuoles/PeroxisomesInteraction of yeast mitochondrial phosphatidylethanolamine with components governing function and morphology of mitochondria.
Sabine Rosenberger, Ruth Nebauer, Ruth Birner-Grünberger, Günther Daum
Institute of Biochemistry, TU Graz, Petersgasse12, Graz, A-8010, Austria (Europe)
105CMitochrondria/Vacuoles/PeroxisomesThe role of Ilv5p interactors in mitochondrial DNA repair.
Rey Sia (1), Anthony Mirando (1), Elaine Sia (2)
(1) Dept. of Biological Sciences, SUNY College at Brockport, 350 New Campus Dr, Brockport, NY, 14420, USA; (2) Dept. of Biology, University of Rochester, RC Box 270211, Rochester, NY 14627
106AMitochrondria/Vacuoles/PeroxisomesThe essential role of mtDNA in the maintenance of an energized inner mitochondrial membrane.
Christopher P. Smith, Peter E. Thorsness
Molecular Biology, University of Wyoming, 1000 E. University A, Laramie, WY, 82072, USA
107BMitochrondria/Vacuoles/PeroxisomesAbsent RNA species in mrs3/4 null mutant mitochondria.
Yan Zhang, Elise R. Lyver, Simon A. B. Knight, Andrew Dancis
Medicine, Div. Hem/Onc, University of Pennsylvania, 421 Curie Blvd., Philadelphia, PA, 19104, USA
108CProtein sorting and turnoverStabilization of yeast Gcn4p in starved cells is dependent on Pcl7p and Pho81p.
Katrin Bömeke, Ralph Pries, Virginia Korte, Gerhard H. Braus
Mol. Microbiology & Genetics, Microbiology & Genetics, Grisebachstr. 8, Göttingen, 37077, Germany
109AProtein sorting and turnoverEffects of ubiquitin system on formation and maintenance of a yeast prion.
Tatiana A. Chernova (1), Kim D. Allen (2), E. Paula Tennant (2), Keith D. Wilkinson (1), Yury O. Chernoff (2)
(1) Department of Biochemistry, Emory University, 1510 Clifton Rd., Atlanta, GA, 30322, USA; (2) School of Biology and IBB, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, GA, 30332, USA
110BProtein sorting and turnoverEssential Role for Ubiquitin Modification and Ubiquitin Recognition in the MVB Sorting Pathway.
Tony Chu (1), David J. Katzmann (2), Srimonti Sarkar (1), Anjon Audhya (1), Christopher Stefan (1), Scott Emr (1)
(1) CMM & HHMI, UCSD, 9500 Gilman Dr., La Jolla, CA, 92093, USA; (2) Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First St. SW, Rochester
111C
Poster Withdrawn/Cancelled
112AProtein sorting and turnoverSelectivity of protein oxidative damage during replicative aging in Saccharomyces cerevisiae.
Nika Erjavec, Thomas Nyström
Dept. of Microbiology, Cell and Molecular Biology, Box 462, Göteborg, 40530, Sweden
113BProtein sorting and turnoverCharacterization of a novel ubiquitin-like protein family involved in multiple intra-cellular processes.
Rina Glozman, Zvulun Elazar, Gad Galili
Biological Chemistry, Weizmann Institute of Science, Hertzel, Rehovot, 76100, Israel
114CProtein sorting and turnoverMolecular characterization of the HpYPS1 and HpYPS7 genes encoding functional yeast aspartyl endoproteases in the methylotrophic yeast Hansenula polymorpha.
Eun Jung Kim (1), Min Jeong Sohn (1), Eun-Young Cho (1), Jeong-Yoon Kim (2), Sang-Ki Rhee (1), Hyun Ah Kang (1)
(1) Korea Research Institute of, Bioscience and Biotechnology, Yusong-gu, Daejeon, 305-600, Korea; (2) Dep. Of Microbiology, Chungnam national University, Daejeon 305-764, Korea.
115AProtein sorting and turnoverThe differential treatment of model misfolded membrane-spanning proteins by components of ER quality control.
Margaret Kincaid, Antony Cooper
School of Biological Sciences, Univ. of Missouri- Kansas City, 5007 Rockhill Road, Kansas City, MO, 64114, USA
116BProtein sorting and turnoverBiochemical Screen for Substrates of Yeast E3 Rsp5.
Bart Kus, Aaron Gajadhar, Daniela Rotin, Aled Edwards
Banting & Best Dept of Med Res, University of Toronto, 112 College st., Toronto, On, M5G 1L6, Canada
117CProtein sorting and turnoverThe role of Sur4p in CAPP-dependent endocytic signaling.
Jeanelle M. Morgan, Joseph T. Nickels
Biochemistry, Drexel Univ. College of Med., 245 N. 15th Street, Philadelphia, PA, 19102, United States
118AProtein sorting and turnoverThe tailpiece of the secretory IgM heavy chain is recognized as a secretion signal by yeast cargo receptors Erv14p and Erv29p.
Guy Nadel, Maya Gordon, Olga Burdelova, Yechiel Elkabetz, Shoshana Bar-Nun
Biochemistry, Tel-Aviv university, Levanon 20, Tel-Aviv, 76352, Israel
119BProtein sorting and turnoverAkr1p-dependent palmitoylation of the Yck2p C-terminus is sufficient for Sec-dependent plasma membrane targeting.
Praveen Babu, Lucy C. Robinson
Biochemistry and Mol. Biology, LSU Health Sciences Center, 1501 Kings Highway, Shreveport, LA, 71130, USA
120CProtein sorting and turnoverSts1: A novel regulator of protein degradation by the ubiquitin/proteasome system.
Lizbeth Romero-Pérez, Dave Lamberston, Li Chen, Kiran Madura
Biochemistry and Molecular Bio, UMDNJ/RWJMS, 683 Hoes Lane, Piscataway, NJ, 08854, USA
121AProtein sorting and turnoverCharacterization of the role of the Ras/PKA pathway in the regulation of autophagy.
Joseph Stephan, Yelena Budovskaya, Paul Herman
Molecular Genetics, Ohio State University, 484 West 12th Av, Columbus, OH, 43210, USA
122BProtein sorting and turnoverThe Yeast Casein Kinase Yck3p Is Palmitoylated, then Sorted to the Vacuolar Membrane with AP-3-dependent Recognition of a YXXΦ Adaptin Sorting Signal.
BEIMENG SUN (1), LINYI CHEN (2), WEI CAO (1), AMY F. ROTH (3), NICHOLAS G. DAVIS (1)
(1) Department of Pharmacology, Wayne State University, 421 E. Canfield, Detroit, MI, 48202, USA; (2) Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109; (3) Department of Surgery, Wayne State Unviersity, School of Medicine, Detroit, MI 48202
123CProtein sorting and turnoverA novel function of Rsp5 ubiquitin ligase: Involvement of degradation of stress-induced abnormal proteins.
Chikara Hoshikawa, Mirei Hisano, Hiroshi Takagi
Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, Fukui, 910-1195, Japan
124ASignal transductionAmino-acid signalling in yeast: casein kinase I and the protease-like Ssy5 protein are key determinants of endoproteolytic activation of the membrane bound Stp1 transcription factor.
Fadi Abdel-Sater, Antonio Urrestarazu, Mohamed El Bakkoury, Bruno André
Molecular Cell Physiology, Free University of Brussels, Pr Jeener & Brachet, Gosselies, 6041, Belgium
125BSignal transductionYeast map kinases with different requirements for a gated scaffold enhance signaling specificity.
Laura Flatauer, Sheena Zadeh, Lee Bardwell
Developmental & Cell Biology, Univ.of California, Irvine, 5205 McGaugh Hall, Irvine, CA, 92697-2300, USA
126CSignal transductionMsg5 and Dig2 affect the nuclear localization of the S. cerevisiae Fus3 MAPK.
Ernest Blackwell, David Stone
Lab for Molecular Biology, University of Illinois-Chicago, 900 S. Ashland Ave., Chicago, IL, 60607, USA
127ASignal transductionGlucose repression mechanism in baker's yeast does not correlate to the ATP:AMP ratio.
Daniel Bosch Ibáñez, Karin Otterstedt, Joakim Norbeck, Christer Larsson, Lena Gustafsson
Molecular Biotechnology, Chalmers University, Medicineragatan 9c, Gothenburg, 40530, Swede
128BSignal transductionEvolutionary proteomics: a method for identifying substrates of the cAMP-dependent protein kinase in S. cerevisiae.
Yelena V. Budovskaya, Paul K. Herman
Molecular Genetics, The Ohio State University, 484 W.12th Avenue, Columbus, OH, 43202, USA
129CSignal transductionGenome-wide screening for genes involved in the regulation of di-/tripeptide transport in Saccharomyces cerevisiae.
Houjian cai, Jeffrey Becker
Department of Microbiology, University of Tennessee, M407 WLS building, Knoxville, TN, 37916, USA
130ASignal transductionRegulation of Glc7 protein phosphatase-1 activity.
John F. Cannon, Anuprita Ghosh
Molecular Micro & Immunology, University of Missouri, 1 Hospital Drive, Columbia, MO, 65212, USA
132CSignal transductionProtein-protein interactions in the HOG pathway - a novel approach.
Ilse Dohnal (1), Suhal M. Salah (2), Gustav Ammerer (2)
(1) Ludwig Boltzmann Forschungsstelle f. Biochemie; (2) Inst. f. Biochem & Mol. Zellb., University of Vienna, Dr. Bohrgasse 9/5, Vienna, 1030, Austria
133ASignal transductionThe role of Bmh and Ssb proteins in glucose repression.
Kenneth M. Dombek, Elton T. Young
Biochemistry, University of Washington, Box 357350, Seattle, WA, 98195-7350, United States
134BSignal transductionDissecting cross talk between yeast signaling pathways: key role of inositol pyrophosphates.
Evelyne Dubois, André Feller, Bart Scherens, Fabienne Vierendeels, Francine Messenguy
Microbiology, Research Institute J-M Wiame, Avenue E. Gryzon, 1, Brussels, 1070, Belgium
135CSignal transductionGenetic, biochemical, and topological characterization of Msb2: discovery of multiple hyperactive alleles.
Ellie S. Graham, Erin K. van Olden, Paul J. Cullen, George F. Sprague
Institute of Molecular Biology, University of Oregon, Franklin Blvd, Eugene, OR, 97403, USA
136ASignal transductionRoles of three upstream kinases in activating different forms of Snf1 protein kinase.
Seung-Pyo Hong, Marian Carlson
Genetics and Development, Columbia University, 701 W. 168th St, HSC, New York, NY, 10032, USA
137BSignal transductionThe invasive growth induced by the absence of the Isw2p-Itc1p chromatin-remodeling complex in Saccharomyces cerevisiae is mediated by activation of the pheromone-response pathway.
Petra Trachtulcova, Ivana Frydlova, Ivana Janatova, Jiri Hasek
Cell. Mol. Microbiol., Institute of Microbiology ASCR, Videnska 1083, Prague 4, 14220, The Czech Republic
138CSignal transductionCharacterization of mouse heavy metal transcription factor MTF-1 in yeast.
Yong Hwan Jin, Hanan Al-Refai, Elena Craft, Jonathan Freedman
NSEES, Duke University, A 304, LSRC, Durham, NC, 27708, USA
139ASignal transductionYeast Ypk protein kinase acts at the downstream of TOR2 pathway.
Yoshiaki Kamada, Yoshinori Ohsumi
Dept. of Cell Biology, Natl. Inst. for Basic Biology, Myodaiji-cho, Okazaki, 444-8585, Japan
140BSignal transductionOxidative stress triggers yeast and human C-type cyclin destruction through activation of a MAP kinase cascade.
Elizabeth Krasley (1), Kun Lee (1), Katrina Cooper (2), Michael Mallory (1), Roland Dunbrock (1), Randy Strich (1)
(1) Cell & Developmental Biology, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA, 19111, USA; (2) Dept. of Biochemistry, Drexel College of Medicine, Philadelphia, PA
141CSignal transductionRole for the conserved S. cerevisiae RAM signaling network in cell wall maintenance.
Cornelia Kurischko, Gretchen Weiss, Francis C. Luca
School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA, 19104, USA
142ASignal transductionA conserved protein interaction network involving the yeast MAP kinases Fus3 and Kss1.
Anasua B.Kusari (1), Douglas M. Molina (2), Walid Sabbagh Jr. (2), Chang S. Lau (2), Lee Bardwell (2)
(1) Biology, Keck Graduate Institute, 535 Watson Drive, Claremont, CA, 91711, USA; (2) Department of Developmental and Cell Biology, University Of California, Irvine, CA 92697
143BSignal transductionFunctional analysis of the Rgt2 glucose sensor.
Hisao Moriya (1), Mark Johnston (2)
(1) JST Kitano Project, The Systems Biology Institute, 6-31-15 Jingumae, Shibuya-ku, Tokyo, 150-0001, Japan; (2) Dept. of Genetics, Washington University School of Medicine, 660 S.Euclid St.Louis MO 63110 USA
144CSignal transductionRegulation of HXT genes in Saccharomyces kluyveri.
Kasper Møller (1), Jens Nielsen (2), Mark Johnston (1)
(1) Genetics, Washington University, 4444 Forest Park, St. Louis, MO, 63108, USA; (2) Center for Microbial Biotechnology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark
145ASignal transductionRegulation of biofilm formation in Saccharomyces cerevisiae by three signal transduction pathways.
Giorgia Pirino (1), Marilena Budroni (1), G. Antonio Farris (1), James R. Broach (2)
(1) DiSAABA, sez. Microbiologia, University of Sassari, viale Italia 39, Sassari, 07100, Italy; (2) Department of Molecular Biology, Princeton University, Princeton, New jersey 08544, USA
146BSignal transductionPhysiological studies of Saccharomyces cerevisiae strains deficient in glucose sensing and mitochondrial respiration.
Vijayendran Raghevendran, Lisbeth Olsson, Jens Nielsen
CMB, Biocentrum-DTU, Søltofts plads, Lyngby, 2800, Denmark
147CSignal transductionSphingolipid-dependent recruitment of the Pkh1 and Pkh2 protein kinases to the plasma membrane.
Françoise Roelants, Sarit Shtivelman, Jeremy Thorner
Dept. of Molec. & Cell Biology, Univ. of California, Berkeley, Rm. 16, Barker Hall, Berkeley, CA, 94702-3202, USA
148ASignal transductionBiochemical analysis of the role of Ste5 in MAP kinase signaling specificity.
Monica Schwartz, Hiten Madhani
Department of Biochemistry, UCSF, 600 16th Street, San Francisco, CA, 94143, USA
149BSignal transductionMutant alleles of the essential 14-3-3 gene, BMH1, in Candida albicans distinguish between growth and filamentation.
Glen Palmer, Sumana Ghosh, Bray Denard, Joy Sturtevant
Microbiol, Immunol & Parasitol, LSUHSC School of Medicine, 1100 Florida Ave, New Orleans, LA, 70117, USA
150CSignal transductionGln3 phosphorylation and intracellular localization in nutrient limitation and starvation differ from those generated by rapamycin-inhibition of Tor1/2 in Saccharomyces cerevisiae.
Kathleen H. Cox, Ajit Kulkarni, Jennifer J. Tate, Terrance G. Cooper
Molecular Sciences, University of Tennessee, 858 Madison Ave., Memphis, TN, 38163, U.S.A.
151ASignal transductionTransmembrane domains of the osmosensor Sln1p regulate signaling.
Gregory Tchou, Paul Smith, Alan Zhang, Bahram Razani, Veronica Flores, Michael Gustin
Biochemistry and Cell Biology, Rice University, 6100 South Main St, Houston, TX, 77005, United States
152BSignal transductionMss11p is central to the regulation of FLO11 gene transcription and invasive growth in Saccharomyces cerevisiae.
Dewald van Dyk (1), Isak S. Pretorius (2), Florian F. Bauer (1)
(1) Inst. for Wine Biotechnology, Stellenbosch University, Victoria Street, Stellenbosch, 7600, South Africa; (2) The Australian Wine Research Institute, Waite Road, Urrbrae, SA 5064 Adelaide, Australia
153CSignal transductionThe interaction of Slt2 MAP kinase with members of the Prs protein family is essential for proper signal transduction through the cell wall integrity pathway in Saccharomyces cerevisiae.
Stefano Vavassori (1), Lilian Schweizer (1), Michael Schweizer (1)
(1) School of Life Sciences , Heriot-Watt University , Edinburgh , EH14 4AS , UK
154ASignal transductionInterplay between Sch9p and the TOR regulated signaling pathway in yeast.
John Wagner, Huma Safdar, Matthew Schechter
Biology, Haverford College, 370 Lancaster Ave., Haverford, PA, 19041, USA
155BSignal transductionNovel alleles of the Schizosaccharomyces pombe cAMP phosphodiesterase gene cgs2+ restore glucose regulation of fbp1+ transcription in an activation-defective adenylate cyclase mutant.
Lili Wang, F. Douglas Ivey, Charles S. Hoffman
Biology Department, Boston College, 140 Commonwealth Ave, Chestnut Hill, MA, 02467, USA
156CSignal transductionModeling pheromone-induced cell polarization in yeast.
Tau-Mu Yi (1), Hiroaki Kitano (2)
(1) Developmental and Cell Biology, UCI, 5205 McGaugh Hall, Irvine, CA, 92697, USA; (2) 6-31-15 Jingumae, M-31 Suite 6A Shibuyaku, Tokyo 150-0001, Japan
157ASignal transductionDissecting the glucose signaling network by genome-wide transcriptional analysis in Saccharomyces cerevisiae.
Shadia Zaman, Ying Wang, James Broach
Molecular Biology, Princeton University, 301 LTL, Princeton, NJ, 08544, USA
158BCell cycle/Growth control/MetabolismCaloric restriction in the yeast Saccharomyces cerevisiae, a stairway to heaven?.
Victor, J. Winter, Rene Verwaal, C. Theo Verrips, Arie, J. Verkleij, Johannes Boonstra
Mol. Cell Biol., University Utrecht, Padualaan 8, Utrecht, 3584 CH, Netherlands
159CCell cycle/Growth control/MetabolismSvf1 regulates oxidative stress response and cell survival in yeast.
Jennifer Brace, David VanderWeele, Charles Rudin
Medical Oncology, Johns Hopkins University, 1650 Orleans Street, Baltimore, MD, 21231, USA
160ACell cycle/Growth control/MetabolismCell cycle dependent nuclear localization of yeast RNase III is required for efficient cell division.
Mathieu Catala, Bruno Lamontagne, Stéphanie Larose, Ghada Ghazal, Sherif Abou Elela
Microbiologie et Infectiologie, Université de Sherbrooke, 12e avenue Nord, Sherbrooke, QC, J1H 5N4, Canada
161BCell cycle/Growth control/MetabolismA microarray-based approach to deciphering cell size control in the budding yeast.
Michael A. Cook, Paul Jorgensen, Kin Chan, Chi Yip Ho, Mike Tyers
Medical Genet. and Microbiol., University of Toronto, SLRI, 600 University Ave., Toronto, ON, M5G 1X5, Canada
162CCell cycle/Growth control/MetabolismLoss of CDC55 in Saccharomyces cerevisiae Causes Synthetic Lethality with GRR1 Mutations.
Paula Da Silva, Joseph Nickels
Biochemistry, Drexel Univ College of Med, 245 N 15th St, Philadelphia, PA, 19102, USA
163ACell cycle/Growth control/MetabolismStress Resistance Genes Limit Replicative Life Span Extension in Saccharomyces cerevisiae.
Paola Fabrizio (1), Scott Pletcher (2), Nadege Minois (2), James Vaupel (2), Valter Longo (1)
(1) Andrus Gerontology Ctr., University of So. California, 3715 McClintock Ave, Los Angeles, CA, 90089, USA; (2) Max Planck Institute for Demographic Research, 18057 Rostock, Germany
164BCell cycle/Growth control/MetabolismRole of nutrient availability and substrate abundance in the regulation of the SCF(Grr1) complex.
Julien P. Fey, Stefan Lanker
Molecular & Medical Genetics, Oregon Health & Science Univ., Sam Jackson Park Rd, Portland, OR, 97201, USA
165CCell cycle/Growth control/MetabolismEvidence for a nuclear autonomous cell cycle driven by stable cyclin proteins.
Amy Gladfelter, Katrin Hungerbuehler, Peter Philippsen
Molecular Microbiology, University of Basel-Biozentrum, Klingelbergstrasse50, Basel, 4056, Switzerland
166ACell cycle/Growth control/MetabolismIdentification of Saccharomyces cerevisiae genes involved in furfural tolerance during fermentation.
Steven Gorsich, Patricia Slininger, Zonglin Liu, Nancy Nichols, Bruce Dien
Crop BioProtection, NCAUR/ARS/USDA, 1815 N. University, Peoria, IL, 61604, USA
167BCell cycle/Growth control/MetabolismGid8p (Dcr1p) and Dcr2p function in a common pathway to promote START completion.
Ritu Pathak, Lydia Bogomolnaya, Jinbai Guo, Michael Polymenis
Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX, 77843, USA
168CCell cycle/Growth control/MetabolismTHE G1 CYCLIN CLN3 CONTROLS VACUOLAR BIOGENESIS.
Bong-Kwan Han, Rodolfo Aramayo, Michael Polymenis
Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX, 77843, USA
169ACell cycle/Growth control/MetabolismThe ARF-GAP Gcs1 is required for adaptation to the DNA damage checkpoint.
Jacob C. Harrison (1), Ayelet Arbel-Eden (1), Chiara Lucca (2), Marco Foiani (2), James E. Haber (1)
(1) Rosenstiel Center, Brandeis University, 415 South St, Waltham, MA, 02454, USA; (2) Universita degli Studi di Milano and Instituto FIRC di Oncologia Molecolare, Milan 20133 Italy
170BCell cycle/Growth control/MetabolismRpg1p/eIF3a of Saccharomyces cerevisiae accumulates in stress granules.
Jiri Hasek (1), Ivana Janatova (1), Martin Spryngar (1), Heimo Wolinski (2), Sepp D. Kohlwein (2)
(1) Cell. Mol. Microbiol., Institute of Microbiology ASCR, Videnska 1083, Prague 4, 14220, The Czech Republic; (2) Institute of Molecular Biosciences, University of Graz, Schubertstrasse 1, Graz, A8010 Austria
171CCell cycle/Growth control/MetabolismThe proteasomal substrate Hel48 is functionally linked to the mitotic exit network.
Harish Karnam (1), Iris Velten (1), Martin Ligr (2), Wolfgang K. Hilt (1)
(1) Institut fuer Biochemie, Universitaet Stuttgart, Pfaffenwaldring 55, Stuttgart, BW, 70569, Germany; (2) present address, Box 25, Center for Studies in Physics and Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
172ACell cycle/Growth control/MetabolismThe Yeast Biochemical Pathways tool: visualization of yeast pathways using the Saccharomyces Genome Database.
J. E. Hirschman, R. Balakrishnan, K. R. Christie, M. C. Costanzo, K. Dolinski, S. S. Dwight, S. R. Engel, D. G. Fisk, E. L. Hong, R. Nash, A. Sethuraman, B. Starr, C. L. Theesfeld, R. Andrada, G. Binkley, Q. Dong, D. Botstein, J.M. Cherry
Department of Genetics, Stanford University, 300 Pasteur Drive, Stanford, CA, 94305-5120, USA
173BCell cycle/Growth control/MetabolismRegulation of mitotic progression by the Mps1p and Bub1p spindle checkpoint kinases.
Eric Holinger (1), Mark Winey (1)
(1) Dept. of MCDBiology, University of Colorado, CB347 Porter B415, Boulder, CO, 80309, USA
174CCell cycle/Growth control/MetabolismEstablishment and maintenance of asynchronous mitosis in multinucleated A.gossypii cells.
A. Katrin Hungerbühler, Peter Philippsen, Amy S. Gladfelter
University of Basel, Biozentrum, Klingelbergstrasse, Basel, 4056, Switzerland
175ACell cycle/Growth control/MetabolismHigh copy suppression analysis of mis-localized G1 cyclin Cln3 in the budding yeast Saccharomyces cerevisiae.
Katherine L. Jameson, Mary E. Miller
Biology, Rhodes College, 2000 North Parkway, Memphis, TN, 38112, USA
176BCell cycle/Growth control/MetabolismSlowed DNA synthesis-induced filamentous growth of S. cerevisiae.
Yiwei Jiang, Chris Kang
Medical Biochemistry and Genet, TAMUSHSC, 428 Raynolds Med Bld, College Station, 77843-1114, USA
177CCell cycle/Growth control/MetabolismMultiple levels of regulation exist to convert Rgt1 from a transcriptional repressor to an activator in response to glucose.
Jeong-Ho Kim, Jeffrey Polish, Mark Johnston
Department of Genetics, Washington University, 4444 Forest Park, St. Louis, MO, 63108, USA
178ACell cycle/Growth control/MetabolismThe CDK-activating kinase Cak1 functions as an assembly factor for Cdc28 mitotic complexes promoting chromosome stability.
Ana Kitazono (1), Stephen Kron (2)
(1) Dept. of Mol. Genetics and Cell Biology and Center for Mol. Oncology, The University of Chicago, 924 East 57th Street R322, Chicago IL 60637; (2) Mol. Genetics and Cell Biology, University of Chicago, 924 E. 57th St. R322, Chicago, IL, 60637, USA
179BCell cycle/Growth control/MetabolismMetabolic engineering to eliminate the Crabtree Effect in Saccharomyces cerevisiae: The AlcoFree Yeast.
Arthur L. Kruckeberg
Research & Development, Gothia Yeast Solutions AB, Terrasssgatan 7, Gothenburg, 41133, Sweden
180CCell cycle/Growth control/MetabolismCharacterization of Gis4: A substrate of the SCFGrr1 Ubiquitin Ligase.
Janna La Rue, Julienne Escamilla, Stefan Lanker
Molecular and Medical Genetics, Oregon Health and Science Univ, 3181 SW Sam Jackson, Portland, OR, 97201, USA
181ACell cycle/Growth control/MetabolismLicensing mitotic entry: Mechanisms of concerted phosphorylation and down-regulation of Swe1 by multiple kinases.
Satoshi Asano, Jung-Eun Park, Li-Rong Yu, Krisada Sakchaisri, Sukgil Song, Porntip Supavilai, Timothy Veenstra, Kyung Lee
Metabolism, National Cancer Institute, 9000 Rockville Pike, Bethesda, MD, 20892-4258, U. S. A.
182BCell cycle/Growth control/MetabolismGetting stuck on you: identifying components of the Smt3 pathway in yeast.
Alaron Lewis, Mark Hochstrasser
Cell Biology, Yale University, 260 Whitney Ave, New Haven, CT, 06520, USA
183CCell cycle/Growth control/MetabolismRelationship between Swe1p localization and function.
Aron Marquitz, Trevin Zyla, Daniel Lew
Pharmacology & Cancer Biology, Duke University, C360 LSRC, Durham, NC, 27710, United States
184ACell cycle/Growth control/MetabolismStructure function analysis of the G1 cyclin Cln3 of Saccharomyces cerevisiae.
Mary E. Miller (1), Frederick R. Cross (2), Alison L. Greoger (1)
(1) Biology, Rhodes College, 2000 North Parkway, Memphis, TN, 38112, United States; (2) The Rockefeller University, New York, NY 10021
185BCell cycle/Growth control/MetabolismRole of protein kinase C in regulating polarized bud growth in Saccharomyces cerevisiae.
Masaki Mizunuma, Dai Hirata, Tokichi Miyakawa
Molecular Biotechnology, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima, 739-8530, Japan
186CCell cycle/Growth control/MetabolismPho85 kinase functions as a nutrient-sensing/responding kinase in a broad area of environmental nutritional conditions.
Masafumi Nishizawa (1), Yuki Katou (2), Katsuhiko Shirahige (2), Akio Toh-e (3)
(1) Dept. Microbiol. and Immunol., Keio Univ. School of Medicine, 35 Shinanomachi, Tokyo, 160-8582, Japan; (2) Human Genome Research Group, Riken Genomic Science Center, Yokohama; (3) Dept. Biological Sciences, Tokyo University Graduate School of Science, Tokyo, Japan
187ACell cycle/Growth control/MetabolismLong-term survival of Saccharomyces cerevisiae colonies: Necessity of metabolism reprogramming.
Libuse Vachova (1), Frederic Devaux (2), Helena Kucerova (1), Marketa Ricicova (3), Zdena Palkova (3)
(1) Institute of Microbiology, Academy of Science CR, Vídeňská 1083, 142 20 Prague 4, Czech Republic; (2) Laboratoire de Genetique Moleculaire, CNRS 8541, Ecole Normale Superieure, 75005 Paris, France; (3) Department of Genetics and Microbiology, Charles University, Vinicna 5, 12844 Prague 2, Czech Republic
188BCell cycle/Growth control/MetabolismLife without polo: Localization-specific mitotic functions of budding yeast polo kinase Cdc5.
Jung-Eun Park, Chong Park, Krisada Sakchaisri, Tatiana Karpova, Satoshi Asano, James McNally, Yangil Sunwoo, Sun-Hee Leem, Kyung Lee
Metabolism, National Cancer Institute, 9000 Rockville Pike, Bethesda, MD, 20892-4258, U. S. A.
189CCell cycle/Growth control/MetabolismDeletion of NAD+-dependent isocitrate dehydrogenases extends lifespan.
Trey Powers (1), Stan Fields(1) (2)
(1) University of Washington, Departments of Genome Sciences and Medicine; (2) Howard Hughes Medical Institute
190ACell cycle/Growth control/MetabolismCell cycle initiation by the Swi6 transcription factor without cyclin dependent kinase activity.
Jonathan B. Schaefer (1), Julia M. Sidorova (2), Linda L. Breeden (1)
(1) Division of Basic Sciences, Fred Hutchinson Cancer Researc, 1100 Fairview Ave N, Seattle, WA, 98109-1024, USA; (2) University of Washington, 1959 NE Pacific St, Seattle WA, 98195
191BCell cycle/Growth control/MetabolismLife without chitin.
Martin Schmidt
Biochemistry and Nutrition, Des Moines University, 3200 Grand Avenue, Des Moines, IA, 50312, USA
192CCell cycle/Growth control/MetabolismRole of Hog1 MAPK in cell-cycle regulation in response to hyperosmotic stress.
Atsunori Shitamukai, Toshinaga Yamaguchi, Dai Hirata, Tokichi Miyakawa
Molecular Biotechnology, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, 739-8530, Japan
193ACell cycle/Growth control/MetabolismYeast stress adaptation: A cost-benefit analysis.
Gertien Smits, Pepijn Boeree, Femke Mensonides, Catarina Resende, Jarne Postmus, Gerco van Eikenhorst, Stanley Brul
Molecular Biology, SILS/University of Amsterdam, Nwe Achtergracht 166, Amsterdam, 1018 WV, The Netherlands
194BCell cycle/Growth control/MetabolismCell division defects of Schizosaccharomyces pombe liz1-mutants are caused by defects in pantothenate uptake.
Juergen Stolz (1), Thomas Caspari (2), Antony Carr (2), Norbert Sauer (3)
(1) Lehrstuhl fuer Zellbiologie, Universitaet Regensburg, Universitaetsstr. 31, Regensburg, D-93040, Germany; (2) Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, UK; (3) Molecular Plant Physiology, Friedrich-Alexander Universitaet Erlangen-Nuernberg, Erlangen, D-91058, Germany
195CCell cycle/Growth control/MetabolismCdc34/SCFMet30 regulates G1/S transition independent of G1-CDK activity.
Ning-Yuan Su, Karin Flick, Peter Kaiser
Dep of Biological Chemistry, UC Irvine, 19182 Jamboree Blvd., Irvine, CA, 92697, USA
196ACell cycle/Growth control/MetabolismInteractions between two genes involved in spindle pole body duplication: MPS1 and CDC31.
Suzanne van Kreeveld Naone (1), Andrea Castillo (2), Mark Winey (1)
(1) MCD Biology, University of Colorado, 347 UCB, Boulder, CO, 80309, USA; (2) MCD Biology, University of California, 225 Sinsheimer, Santa Cruz, CA 95064
197BCell cycle/Growth control/MetabolismAnalysis of Cbk1p regulatory sites.
Margaret Barry, Myra Sutanto, Eric Weiss
BMBCB, Northwestern University, 2205 Tech Drive, Evanston, IL, 60208, United States
198CCell cycle/Growth control/MetabolismIdentification of novel proline rich motifs (PRM) required for interaction between Vrp1p and Hof1p, a PCH family protein member involved in cytokinesis.
Gang REN (1), Barbara Winsor (2), Alan MUNN (3)
(1) FRE2375 du CNRS, Strasbourg and Institute for Molecular Bioscience, The University of Queensland; (2) FRE2375, IBMC du CNRS, 15 rue Descartes, Strasbourg, 67084, France; (3) Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, AUSTRALIA
199ACell cycle/Growth control/MetabolismRoles of Yap1p in the Ca2+-induced G2 cell-cycle regulation.
Hiroshi Yokoyama, Masaki Mizunuma, Dai Hirata, Tokichi Miyakawa
Graduate School of ADSM, Hiroshima University, 1-3-1, Higashi-Hiroshima, 739-8530, Japan
200BCell cycle/Growth control/MetabolismBiofilm formation of Saccharomyces cerevisiae wine yeast strains is related to stress responses.
Giacomo Zara, Severino Zara, Monica Sechi, Marilena Budroni
Di.S.A.A.B.A., University of Sassari, v.le Italia, 39, Sassari, 07100, Italy
201CMating/Sporulation/MeiosisFRET analysis of signaling dynamics in the Saccharomyces cerevisiae mating response.
Peggy Baudouin-Cornu, Mike Tyers
SLRI, Mount Sinai Hospital, University Avenue, Toronto, ON, M5G1X5, Canada
202AMating/Sporulation/MeiosisRole of Glc7p in polarized morphogenesis during mating.
Jennifer P. Bharucha (1), James Konopka (2), Kelly Tatchell (1)
(1) Biochemistry & Mol. Biology, LSUHSC, Kings Hwy, Shreveport, LA, 71130, U.S.A.; (2) Department of Molecular Genetics and Microbiology, SUNY Stony Brook, Stony Brook, NY 11794.
203BMating/Sporulation/MeiosisDistinct roles for RSC1 and RSC2 in controlling the mid-late class of sporulation specific genes in Saccharomyces cerevisiae.
David Bungard, Edward Winter
Biochemistry, Thomas Jefferson University, 233 South 10th st, Philadelphia, PA, 19107, USA
204CMating/Sporulation/MeiosisThe role of the spindle checkpoint in yeast meiosis.
Peter Cheslock (1), Ben Kemp (2), Dean Dawson (1)
(1) Genetics, Tufts University, 136 Harrison Avenue, Boston, Ma, 02111, USA; (2) Dartmoth College Biology Dept, 115 Gilman Hall, Hanover, NH 03755 USA
205AMating/Sporulation/MeiosisIme4 promotes sporulation by methylating adenosine residues in polyA RNA.
Mary Clancy, Candace Timpte
Biological Sciences, University of New Orleans, Lakefront, New Orleans, LA, 70148, USA
206BMating/Sporulation/MeiosisFunctional Analysis of Gcs1p in Sporulation.
Jaime E. Connolly (1), JoAnne Engebrecht (2)
(1) Department of Pharmacology, Stony Brook University, Health Sciences Cntr, Stony Brook, NY, 11794-8651, United States; (2) Molecular and Cellular Biology Section, University of California at Davis, Davis, CA 95616
207CMating/Sporulation/MeiosisRole Of PP2A In Regulating Meiosis.
Christina Gallo, Joseph Nickels
Biochemistry, Drexel Univ. College of Med., 245N 15th street, philadelphia, pa, 19102, USA
208AMating/Sporulation/MeiosisIdentification of proteins that interact with the alpha-factor mating pheromone receptor (Ste2p), using the split ubiquitin two hybrid assay.
Douglas P. Gladue, James B. Konopka
Mol. Genetics & Microbiology, SUNY at Stony Brook, 130 Life Sciences, Stony Brook, NY, 11794-5222, USA
209BMating/Sporulation/MeiosisExpression and interaction of Cdc7 and Dbf4 proteins in S.cerevisiae meiosis.
Seth A. Hanson, Joshua H. Olson, Anne M. Galbraith
Biology Department, UW- La Crosse, 1725 State St., La Crosse, WI, 54601, USA
210CMating/Sporulation/MeiosisSynaptonemal complex initiates at the subset of double-strand breaks destined to become crossovers.
Kiersten Henderson, Scott Keeney
Molecular Biology, Sloan-Kettering Institute, 430 E. 67th Street, NYC, NY, 10021, USA
211AMating/Sporulation/MeiosisS. cerevisiae as a model system for studying the interaction of the C. albicans alpha-mating pheromone and its receptor.
A.M. Janiak (1), H. Sargsyan (2), J. Russo (2), F. Neider (2), J.M. Becker (1)
(1) Department of Microbiology, University of Tennessee, 1414 Cumberland Ave., Knoxville, TN, 37996, USA; (2) Department of Chemistry, College of Staten Island, CUNY, Staten Island, NY
212BMating/Sporulation/MeiosisGlucose and Nitrogen regulate the switch from histone deacetylation to acetylation for expression of early meiosis-specific genes in budding yeast.
Lilach Pnueli (1), Ifat Rubin-Bejerano (2), Shira Sagee (1), Osnat Friedman (1), Iris Edry (3), Miriam Cohen (4), Yona Kassir (1)
(1) Deaprtment of Biology, Technion, Technion City, Haifa, 32000, Israel; (2) Whitehead Institute for Biomedical research Cambridge MA; (3) Department of Biological Regulation, Weizmann Institute of Science, Rehovot Israel; (4) Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot Israel
213CMating/Sporulation/MeiosisThe role of endocytosis in spore formation in Saccharomyces cerevisiae.
Masayo Morishita (1), JoAnne Engebrecht (2)
(1) Section of Molecular and Cellular Biology, UC, Davis, One Shields Avenue, Davis, CA95616, USA; (2) Molecular and Cellular Biology, University of California, Davi, One Shields Avenue, Davis, CA, 95616, USA
214AMating/Sporulation/MeiosisAnalyzing the role of the CDC7 and DBF4 genes in meiosis in Saccharomyces cerevisiae.
Glenn Morse, Matthew Lurken, Allison Maass, Rebecca Rohrer, Nicole Skoog, Peter Thielen, Jaren Thomas, Anne Galbraith
Biology, Univ of Wisconsin - La Crosse, 1725 State St., La Crosse, WI, 54601, USA
215B
Poster Withdrawn/Cancelled
216CMating/Sporulation/MeiosisThe activity of meiosis-specific protein kinase Ime2 is regulated by tyrosine phosphorylation.
Matthew Rawluk, Alastair McKean, Catherine Hui, David Stuart
Biochemistry, University of Alberta, 5-61 Med Sci Bldg, Edmonton, AB, T6G 2H7, Canada
217AMating/Sporulation/MeiosisThe importance of developmental gene repression mechanisms.
Mayfebelle Reodica, Melissa J. Straffon, Ian W. Dawes
School of Biotech & Biomol Sci, University of New South Wales, Sydney, New South Wales, 2052, Australia.
218BMating/Sporulation/MeiosisLoss of meiotic re-replication block in yeast cells defective in Cdc28p regulation.
Lyndi Rice, Constitine Plakas, Joseph Nickels
Biochemistry, Drexel Univ. College of Med., 245 N. 15th St., Philadelphia, PA, 19102, USA
219CMating/Sporulation/MeiosisRim101 and Nrg1 bind to a bipartite regulatory element to direct repression of DIT1 and DIT2 during vegetative growth in Saccharomyces cerevisiae.
Karen Rothfels (1), Jason Tanny (2), Eniko Molnar (2), Cosimo Commisso (3), Helena Friesen (3), Jacqueline Segall (3)
(1) Department of Biochemistry, University of Toronto, Toronto, ON; (2) Department of Molecular and Medical Genetics, University of Toronto, Toronto, ON; (3) Department of Biochemistry, University of Toronto, 1 King's College, Toronto, ON, M5S 1A8, Canada
220AMating/Sporulation/MeiosisAnalysis of regulatory modifications to Ime2 during meiosis.
Karen Schindler, Edward Winter
Biochemistry, Thomas Jefferson University, 233 S. 10th St., Philadelphia, PA, 19107, USA
221BMating/Sporulation/MeiosisAPC/CAma1 regulates the destruction of the transcriptional repressor Sum1p during meiosis..
Reza Shirzadi, Katrina Cooper
Biochemistry, Drexel University - COM, 215 N 15. MS 497 NCB, Philadelphia, PA, 19102, USA
222CMating/Sporulation/MeiosisAPC/CAma1p control of meiotic progression.
Grace S. Tan, Katrina F. Cooper
Biochemistry, Drexel U. College of Medicine, 245 N.15th St. MS497, Philadelphia, PA, 19102, USA
223AMating/Sporulation/MeiosisAtf1-Pcr1-M26 complex links stress-activated MAP kinase and cAMP-dependent kinase pathways via chromatin remodeling of cgs2+.
Mari K. Davidson (1), Harish K. Shandilya (1), Kouji Hirota (2), Kunihiro Ohta (2), Wayne P. Wahls (1)
(1) Biochemistry and Mol. Biol., University of Arkansas Med Sci, 4301 West Markham, Little Rock, AR, 72205, USA; (2) Genetic Dynamics Research Unit, RIKEN Institute, Wako, Saitama, Japan
224BMetabolism/membrane traffickingBenzoic acid exerts selective effects on intracellular membrane trafficking pathways in Saccharomyces cerevisiae.
Reut Hazan, Alexandra Levine, Hagai Abeliovich
Biochemistry and Food Science, Hebrew University, P.O. box 12, Rehovot, 76100, Israel
225CMetabolism/membrane traffickingType I myosins, Myo3p and Myo5p, interact with the C-terminus of the endocytic scaffold Pan1p.
Sarah L. Barker, Beverly Wendland
Department of Biology, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, US
226AMetabolism/membrane traffickingYpt31/32 GTPases and their novel F-Box effector protein Rcy1 regulate protein recycling.
Shu Hui Chen, Shan Chen, Fengli Liu, Gregory Jedd, Nava Segev
Biological Sciences, University of Illinois-Chicago, 900 S. Ashland Ave., Chicago, IL, 60607, USA
227BMetabolism/membrane traffickingPoster Award: Honorable Mention  The role of Mon2p in membrane trafficking, protein sorting, and cell growth.
Jem A. Efe, Scott D. Emr
Division of Biology & HHMI, UC San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0668, USA
228CMetabolism/membrane traffickingThe S. cerevisiae acid trehalase is a periplasmic protein with an unusual secretion behavior.
Matthieu Jules, Jean Luc Parrou, Jean M François
Bioengineering Center Gilbert, Depart. Genie Biochimique, 135, Avenue de Range, Toulouse, 31077, France
229AMetabolism/membrane traffickingCharacterization of two yeast gene families whose members regulate TTG resistance.
Kangze He, Aaron Yang, Virginia Aberdeen, William Starmer, Scott Erdman
Department of Biology, Syracuse University, 130 College Place, Syracuse, NY, 13244-1220, USA
230B
Poster Withdrawn/Cancelled
231CMetabolism/membrane traffickingHydrophobicity sorts proteins to the lipid droplet.
Klaus Natter, Julia Petschnigg, Iskandar Dib, Sepp D. Kohlwein
Molecular Biosciences, University Graz, Schubertstr. 1, Graz, A8010, Austria
233BMetabolism/membrane traffickingThe role of the yeast inositol polyphosphate 5-phosphatases in the regulation of vesicular trafficking.
Lisa Ooms (1), Fenny Wiradjaja (1), Alan Munn (2), Robert Piper (3), Peter Mayinger (4), Christina Mitchell (1)
(1) Department of Biochemistry, Monash University, Wellington Rd, Clayton, 3800, Australia; (2) Institute of Molecular and Cell Biology, National University of Singapore, Singapore 117604, Singapore; (3) Department of Physiology and Biophysics, University of Iowa, Iowa City, 52242 IA, USA; (4) Zentrum fur Molekulare Biologie, Universitat Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
234CMetabolism/membrane traffickingPolyunsaturated fatty acid synthesis of yeast.
Takahiro Oura, Kyoko Watanabe, Hiromichi Sakai, Susumu Kajiwara
Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226-8501, Japan
235AMetabolism/membrane traffickingGlycerophosphoinositol transport in yeast.
Jana L. Patton-Vogt, Claudia Almaguer, Wei Cheng, Christi Nolder
Biological Sciences, Duquesne University, 600 Forbes Ave., Pittsburgh, PA, 15282, United States
236BMetabolism/membrane traffickingFunctional analysis of a pleotropic drug resistance (PDR) transporter from the soybean pathogen Phytophthora sojae in yeast transporter mutants.
Yasuko Sakihama (1), Vipaporn Phuntumart (1), Paul Morris (1)
(1) Biological Sciences, Bowling Green State University, Bowling Green, OH 43403
237CMetabolism/membrane traffickingThe Fps1p homologue of the osmotolerant yeast Zygosaccharomyces rouxii.
Xueming Tang, Gerald Kayingo, Bernard A Prior
Department of Microbiology, University of Stellenbosch, Matieland, Stellenbosch, 7602, South Africa
238AMetabolism/membrane traffickingPurine analog sensitivity of the class E VPS mutants of Saccharomyces cerevisiae.
Jennifer Urbanowski, Sarah Connolly, Ronda Rolfes
Department of Biology, Georgetown University, 37th and O Street NW, Washington, DC, 20057-1229, USA
239BCell Biology: OtherThe metabolic role of yeast carnitine acetyl transferases.
Sven Kroppenstedt (1), Jaco Franken (1), Isak S. Pretorius (2), Jan H. Swiegers (2), Florian F. Bauer (1)
(1) Inst. Wine Biotechnology, Stellenbosch University, Victoriastreet, Stellenbosch, 7602, South Africa; (2) Australian Wine Research Institute, Waite Road , Urrbrae Glen Osmond , Adelaide, SA 5064 , Australia
240CCell Biology: OtherTwo isoforms of yeast Hsp70 chaperones are required for different prions [PSI+] and [URE3] propagation in a distinctive way.
Seyung Chung, Daniel C. Masison
LBG, NIDDK, NIH, 9000 Rockville Pike, Bethesda, MD, 20892, U.S.A.
241ACell Biology: OtherThe cell wall protein Flo11/Muc1 is an adhesin that exhibits homotypic interactions.
Anne Dranginis (1), Lois Douglas (1), Jennifer Bayly (2), Li Li (1), Sreevardhini Venkatraman (1)
(1) Biological Sciences, St. John's University, 8000 Utopia Parkway, Jamaica, NY, 11439, USA; (2) University of Stellenbosch, Stellenbosch, South Africa
242BCell Biology: OtherFLO8 dependent adhesion in the S288C derived EUROSARF (BY) strain background.
Lars Fichtner, Gerhard H. Braus
Molecular Microbiology, Georg-August-University, Grisebachstr. 8, Goettingen, D-37077, Germany
243CCell Biology: OtherIdentification of genes that regulate resistance to chemotherapeutic ruthenium complexes.
C. Hunter Russell, Brian P. Dranka, George A. Nelson, Laura K. Stultz, Pamela K. Hanson
Biology, Birmingham-Southern College, 900 Arkadelphia Rd., Birmingham, AL, 35254, U.S.
244A?: OtherPhenotypic analysis of the mKir2.1 channel activity in potassium influx and efflux defective Saccharomyces cerevisiae strains.
Guido Hasenbrink (1), Lucie Kolacna (2), Sarah Schwarzer (1), Jost Ludwig (3), Hana Sychrova (2), Hella Lichtenberg-Frate (1)
(1) IZMB, Molecular Bioenergetics, University of Bonn, Kirschallee 1, Bonn, 53115, Germany; (2) Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic; (3) Physiologisch-chemisches Institut, Universität Tübingen, Hoppe-Seyler-Str. 4, 72076 Tübingen, FRG
245BCell Biology: OtherStructural and functional analysis of the polar landmark proteins Bud8p and Bud9p in Saccharomyces cerevisiae.
Anne-Brit Obermayer, Maria Meyer, Hans-Ulrich Mösch
Dept. Gen. Euk. Microorg., Inst. of Microbiol. and Gen., Grisebachstr. 8, Göttingen, D-37077, Germany
246CCell Biology: OtherExpression of mammalian aquaporins in yeast: structure/function analysis and control of osmotic reponses.
Nina Pettersson, Sara Karlgren, Bodil Nordlander, Stefan Hohmann
Cell- and Molecular Biology, Göteborg University, Box 462, Göteborg, 40530, Sweden
247ACell Biology: OtherBni4p involvement in septum formation.
María Sanz, Ángel Durán, César Roncero
Dept. Microbiología y Genética, Univ. Salamanca/CSIC, Av. Campo Charro s/n, Salamanca, 37007, SPAIN
248BCell Biology: OtherKex1p-processing is important for in vivo toxicity of intracellular expressed alpha-subunits derived from yeast killer toxins K1 and K28.
Tanja Sendzik, Jochen Reiter, Frank Breinig, Manfred Schmitt
Applied Molecular Biology, Saarland University, Building 2, Saarbruecken, 66123, Germany
249CCell Biology: OtherThe function of the yeast molecular chaperone Sse1 is mechanistically distinct from the closely related Hsp70 family.
Lance Shaner (1), Amy Trott (1), Jennifer Goeckeler (2), Jeffrey Brodsky (2), Kevin Morano (1)
(1) Micro. and Mol. Genet., Univ. Texas Med. Schl. Houston, 6431 Fannin, Houston, TX, 77030, USA; (2) Dept. Biological Sciences, Univ. Pittsburgh, Pittsburgh, PA 15260
250ACell Biology: OtherAdaptation of Saccharomyces cerevisiae to lipophilic weak acids is mediated by the Msn2/4p-regulated SPI1 gene.
Alexandra R. Fernandes (1), Tania Simoes (2), Inês Canelhas (2), Isabel Sá-Correia (2)
(1) Biolog. Scienc. Research Group, IST; (2) Biolog. Scienc. Research Group, Instituto Superior Técnico, Av Rovisco Pais, Lisboa, 1049-001, Portugal
251BCell Biology: OtherThe yeast QDR1, QDR2 and QDR3 genes, encoding plasma membrane drug H+-antiporters: function as quinidine resistance determinants and differential expression patterns.
Rita C. Vargas, Sandra Tenreiro, Miguel C. Teixeira, Alexandra R. Fernandes, Isabel Sá-Correia
BSRG, Instituto Superior Técnico, Av. Rovisco Pais, Lisboa, 1049-001, Portugal
252CCell Biology: OtherGenome-wide screens for non-essential chromosome stability mutants in S. cerevisiae.
Karen WY Yuen (1), Cheryl D Warren (2), Teresa Kwok (1), Ou Chen (2), Phil A Hieter (1), Forrest A Spencer (2)
(1) Medical Genetics, CMMT, U. of British Columbia, 950 West 28th Avenue, Vancouver, BC, V5Z 4H4, Canada; (2) McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD 21205
253AReplicationInvestigating the role of S. cerevisiae CLB5 in premeiotic DNA replication.
James DeCesare, Lisa Yu, Chantelle Sedgwick, David Stuart
Biochemistry, University of Alberta, 561 Med. Sci. Bldg., Edmonton, AB, T6G 2H7, Canada
254BReplicationAnalysis of DNA damage responses and checkpoint function in chromatin assembly mutants of Saccharomyces cerevisiae.
Ellen Kats, Richard Kolodner
Genetics, Ludwig Institute, 9500 Gilman Dr., San Diego, CA, 92093, United States
255CReplicationCdc45 plays a critical role in suppressing Top1 poisons in yeast and human cells.
Cynthia S. Lancaster, Robert C.A.M. van Waardenburg, Changxian Shen, Alice A. Gibson, Mary-Ann Bjornsti
Molecular Pharmacology, St Jude Children's Rsrch Hosp, 332 N Lauderdale St, Memphis, TN, 38105, USA
256AReplicationExpansion of CAG repeat tracts in DNA ligase I mutants.
Eric Refsland, Haeyoung Kim, Dennis Livingston
Biochem., Mol. Bio. & Biophys., University of Minnesota, 321 Church St. SE, Minneapolis, MN, 55455, USA
257BTelomeresShort telomeres in Vitamin B6 salvage pathway mutants.
Syed Askree, Joshua Hawk, Michael McEachern
Department of Genetics, University of Georgia, Life Sci. Building, Athens, GA, 30606, USA
258CTelomeresA secondary structure for telomerase RNA in the yeast Saccharomyces cerevisiae.
Alain T. Dandjinou, Nancy Lévesque, Sherif Abou Elela, Raymund J. Wellinger
Département de Microbiologie, Université de Sherbrooke, 3001, 12e Av. Nord, Sherbrooke, Qc, J1H 5N4, Canada
259ATelomeresBiochemical reconstitution and analysis of the telomerase holoenzyme in Saccharomyces cerevisiae.
Nancy Laterreur (1), Sherif Abou Elela (2), Raymund J. Wellinger (2)
(1) 3001, 12th avenue N., Sherbrooke, Qc, Canada, J1H 5N4; (2) Microbiology and Infectiology, University of Sherbrooke, 3001, 12th avenue N, Sherbrooke, Qc, J1H 5N4, Canada
260BTelomeresLinear and circular genomes in mitochondria of the yeast Candida parapsilosis.
Jozef Nosek, Martin Kucej, Adriana Rycovska, Silvia Petreszelyova, Matus Valach, Lubomir Tomaska
Dept. Biochemistry & Genetics, Comenius University, Mlynska dolina CH-1, Bratislava, 842 15, Slovak republic
261CTelomeresImportance of the Yku heterodimer subunits in telomere functions.
Mélissa Ricard, Raymund J. Wellinger
Microbiologie et Infectiologie, Université de Sherbrooke, 3001 12e Avenue Nord, Sherbrooke, Qc, J1H 5N4, Canada
262ATelomeresAmplification of telomeric arrays via rolling-circle mechanism.
Lubomir Tomaska (1), Alexander M. Makhov (2), Lubomir Lanator (1), Darina Luknarova (1), Jack D. Griffith (2), Jozef Nosek (1)
(1) Dept. Biochemistry & Genetics, Comenius University, Mlynska dolina B-1, Bratislava, 84215, Slovak Republic; (2) Lineberger Comprehensive Cancer Center, University of North Carolina, Mason Farm Road, Chapel Hill, NC 27599-7295, USA
263BTelomeresNovel genes involved in telomere maintenance grouped into epistasis groups regarding known telomere genes.
Tal Yehuda, Martin Kupiec, Anat Krauskopf
molecular microbiology, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, 69978, Israel
264CCentromeresCBF3 kinetochore protein abundance and function determine the temperature-sensitivity of ctf13-30.
Jeffrey N. Dahlseid (1), Amanda Stonehouse (1), Erin E. Carlson (2), Anita E. Wichmann (3)
(1) Biology and Chemistry, Gustavus Adolphus College, 800 West College Ave, Saint Peter, MN, 56082, USA; (2) Department of Chemistry, University of Wisconsin, Madison, WI 53706; (3) Department of Chemistry, St. Olaf College, Northfield, MN 55057
265ACentromeresDistinct domains of spindle checkpoint protein Mad1p mediate nuclear pore association and spindle checkpoint/chromosome transmission functions.
James Kastenmayer (1), Marina Lee (2), Andrew Hong (3), Forrest Spencer (2), Munira Basrai (3)
(1) Genetics Branch, Center for Cancer Research, National Cancer Institute/National Institutes of Health, Bldg. 8. Rm 5101, 8901 Wisconsin Ave, Bethesda, MD 20889-5101; (2) Molecular Biology and Genetics, John's Hopkins School of Medicine, Ross Bldg.8, 720 Rutland Ave, Baltimore, MD 21205; (3) Genetics Branch, CRC, National Cancer Center, NIH, 8901 Wisconsin Ave, Bethesda, MD, 20889-5101, USA
266BCentromeresS. cerevisiae Spt4p is a component of kinetochores and heterochromatic loci with roles in chromosome segregation and gene silencing.
Yeonjung Kim (1), Luciana B. Crotti (1), Laura MacColl (2), Grant Hartzog (2), Munira A. Basrai (1)
(1) National cancer institute, National institutes of health, 8901 Wisconsin Ave., Bethesda, MD, 20889-5101, USA; (2) MCD Biology, 349 Sinsheimer Labs, University of California, Santa Cruz, CA 95064
267CCentromeresThe protein phosphatase-1 Glc7 promotes spindle checkpoint exit.
Benjamin A. Pinsky, Sean Y. Tatsutani, Sue Biggins
Division of Basic Sciences, FHCRC, 1100 Fairview Ave N., Seattle, WA, 98109, USA
268ATranspositionSaturation mutagenesis of cis-acting signals required for Ty1 retrotransposition.
Candice Coombes, Eric Bolton, Yolanda Eby, Jef Boeke
Molecular Biology and Genetics, Johns Hopkins University, 725 N Wolfe Street, Baltimore, MD, 21205, United States
269BTranspositionInduction of Ty1 at high temperature by deletion of RFX1, a repressor of damage-inducible genes.
Mary Heaton, Jill Keeney
Biology, Juniata College, 1700 Moore St., Huntingdon, PA, 16652, USA
270CTranspositionMutagenic screen for Ty1-tRNA primer interaction.
Mark B. Lawery, Jill B. Keeney
Biology, Juniata College, 1700 Moore St., Huntingdon, PA, 16652, USA
271ATranspositionThe prolyl isomerase FPR1, is required for efficient Ty1 transposition in S. cerevisiae.
Anuradha Sundararajan (1), Jae-Yong Cho (2), David J. Garfinkel (1)
(1) Movable Genetic Elements, National Cancer Institute, Boyles street, Frederick, MD, 21702, USA; (2) Department of Bioindustry and Technology, Sangji University, Kangwon-do 220-702, Korea
272BTranspositionPossible communication between Reverse Transcriptase and Rnase H during reverse transcription.
Robert Yarrington, Jichao Chen, Eric Bolton, Jef Boeke
MBG, Johns Hopkins University SOM, 725 North Wolfe St, Baltimore, MD, 21205, USA
273CRecombinationDiploid deletion mutants with increased frequency of LOH mimic old cell genomic instability.
Daisy Andersen, Daniel E Gottschling
Basic Sciences, Fred Hutchinson CRC, 1100 Fairview Ave N, seattle, WA, 98109, United States
274ARecombinationOrigin(s) of one-sided double-strand break repair.
Stephen A. Banse, Grace M. Wang, Henriette M. Foss, Franklin W. Stahl, Barclay L. Browne
Institute of Molecular Biology, University of Oregon, 1370 Franklin Blvd, Eugene, OR, 97403, USA
275BRecombinationStudies of the relationship between DNA replication and formation of DNA double-strand breaks during meiosis.
Cyril Buhler, Michael Lichten
Laboratory of Biochemistry, National Cancer Institute, 37 Convent drive, Bethesda, MD, 20892-4255, U.S.A.
276CRecombinationVisualization of a genetic interaction: subnuclear localization of Rdh54 and Rad54 in mitotic cells.
Rebecca Burgess, Michael Lisby, Rodney Rothstein
Genetics and Development, Columbia University, 701 W 168th St 1606, New York, NY, 10032, USA
277ARecombinationSaccharomyces cerevisiae MEC1 suppresses general homologous recombination but is required for DNA damage-associated sister chromatid exchange.
Michael Fasullo, Mingzeng Sun
Cancer Research Division, Ordway Research Institute, 150 New Scotland Ave, Albany, NY, 12208, United States
278BRecombinationSaccharomyces cerevisiae G2 checkpoint and recombinational repair mutants exhibit a synergistic increase in chromosomal translocations.
Michael Fasullo, Li Zeng
Cancer Research Division, Ordway Research Institute, 150 New Scotland Ave, Albany, NY, 12208, United States
279CRecombinationPoster Award: Honorable Mention  Examination of the physical properties and genetic requirements of mating type interconversion.
Peter Houston, Eugenia Xu, James Broach
Dept. of Molecular Biology, Princeton University, LTL 301, Princeton, NJ, 08540, United States of America
280ARecombinationThe Sgs1 helicase does not suppress crossover formation during meiotic recombination in Saccharomyces cerevisiae.
Lea Jessop, Michael Lichten
Laboratory of Biochemistry, National Cancer Institute, NIH, 37 Convent Dr37/6124, Bethesda, MD, 20892-4255, USA
281BRecombination'Is the intermediate in mitotic recombination a double strand break?'.
Gaëlle Lettier (1), Adriana Antunez de Mayolo (2), Michael Lisby (2), Qi Feng (2), Naz Erdeniz (2), Rodney Rothstein (2), Uffe H. Mortensen (1)
(1) CMB, Biocenrum-DTU, Søltofts Plads, Lyngby, 2800, Denmark; (2) Department of Genetics and Development, Columbia University, USA
282CRecombinationEvidence for synthesis-dependent strand annealing and multiple rounds of partner engagement during meiotic recombination.
Melissa S. McMahill (1), Caroline W. Sham (2), Jeremy M. Grushcow (3), Douglas K. Bishop (4)
(1) Committee on Genetics, University of Chicago, 920 E. 58th St., Chicago, IL, 60637, USA; (2) Department of Radiation and Cellular Oncology, University of Chicago, Chicago, IL 60637, USA; (3) Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA; (4) Department of Radiation and Cellular Oncology, and Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
283ARecombinationIs chromosome fragment missegregation responsible for age-induced genomic instability?.
Michael A. McMurray (1), Daniel E. Gottschling (2)
(1) Division of Basic Sciences, Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, Seattle, WA 98109; (2) Division of Basic Sciences, Fred Hutchinson CRC, 1100 Fairview Ave N, Seattle, WA, 98109, United States
284BRecombinationSecuring the Securin: Mnd2 is an inhibitor of the meiotic APC, preventing premature cohesin destruction.
Alexandra Penkner (1), Marco Antonio Mendoza (1), Franz Klein (1)
(1) Institute of Botany, Dept. of Cytology and genetics, Rennweg 14 Vienna-Austria
285CRecombinationEpigenetically inherited high frequency mitotic recombination and genome instability induced by mutations in EST2.
Torsten Nilsson-Tillgren
Institute of Molecular Biology, University of Copenhagen, Oester Farimagsgade, Copenhagen, DK-1353, Denmark
286ARecombinationNuclear localization of Rad52 in Saccharomyces cerevisiae.
Iben Plate (1), Michael Lisby (2), Rodney Rothstein (2), Uffe H. Mortensen (1)
(1) Center for Microbial Biotechnology, Technical University of Denmark; (2) Department of Genetics and Development, Colombia University, USA
287B
Poster Withdrawn/Cancelled
288CMutagenesis/RepairTAP tag method to identify Msh4p, Msh5p partners in DNA double strand break repair.
Nelusha Amaladas, Peggy Hsieh
Genetics & Biochemistry Branch, National Institutes of Health, 5 Memorial Drive, Bethesda, MD, 20892-0538, USA
289AMutagenesis/RepairZymocin-induced DNA damage is lethal in the yeast Saccharomyces cerevisiae.
Tammy Westmoreland, John Olson, William Saito, Jeffrey Marks, Craig Bennett
Dept. of Surgery, Duke University Medical Center, 355 MSRB, Durham, NC, 27710, USA
290BMutagenesis/RepairThe RSC chromatin-remodeling complex functions in DNA repair.
Bob Chai, Brehon C. Laurent
Microbiology and Immunology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY, 11203, USA
291CMutagenesis/Repairpms1 alleles with novel effects on mutation avoidance.
Naz Erdeniz, Micheal Liskay
Molecular and Medical Genetics, OHSU, 3181 SW Sam Jackson, Portland, OR, 97239, USA
292AMutagenesis/RepairThe endonuclease activity of RAD1-RAD10 complex is crucial for the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae.
Ji-Young Hwang, Stephanie Smith, Kyungjae Myung
NHGRI, NIH, 49 Convent Drive, Bethesda, MD, 20892, USA
293BMutagenesis/RepairRNASE H2 FROM SACCHAROMYCES CEREVISIAE: A TRIMERIC ENZYME WITH DISTINCT ACTIVITY.
Ho-Sang Jeong, Robert Crouch
LMG, NICHD/NIH, 6 Center Dr, Bethesda, MD, 20892, USA
294CMutagenesis/RepairCheckpoint mutations suppress the slow growth phenotype of 3' phosphatase-deficient yeast .
Anandi Karumbati, Thomas Wilson
Department of Pathology, University of Michigan, 1301 Catherine Rd, Ann arbor, MI, 48109, USA
295AMutagenesis/RepairTranscription-associated mutation at the CAN1 locus in Saccharoymyces cerevisiae.
Ryan P. Larson, Malcolm J. Lippert
Biology Department, Saint Michael's College, 1 Winooski Park, Colchester, VT, 05439, USA
296BMutagenesis/RepairGenome-wide survey of DNA-damage response pathways via functional profiling in yeast.
William Lee (1), Robert P. St.Onge (2), Ronald W. Davis (2), Corey Nislow (2), Guri Giaever (2)
(1) Genetics, Stanford University, Clark Center W300, Stanford, CA, 94305, USA; (2) Stanford Genome Technology Center, 855 California Ave., Palo Alto, CA 94304
297CMutagenesis/RepairPhenotypic yeast analysis for mutagenesis repair and cytotoxicity assessment.
Marcel Schmitt (1), Georg Gellert (2), Jost Ludwig (3), Hella Lichtenberg-Frate (1)
(1) Molecular Bioenergetics, University of Bonn, Kirschallee 1, Bonn, D-53115, Germany; (2) Staatliches Umweltamt Siegen, Unteres Schloss, 57 072 Siegen, Germany; (3) Physiologisch-Chemisches Institut, Universität Tübingen, Hoppe-Seyler-Str. 3, 72064 Tübingen, Germany
298AMutagenesis/RepairNew Genes for Suppression of Gross Chromosomal Rearrangements (GCRs) Identified by Genome-Wide Screening in Saccharomyces cerevisiae.
Stephanie Smith, Ji-young Hwang, Soma Banerjee, Anju Majeed, Amitabha Gupta, Kyungjae Myung
Genetics and molecular biology, NHGRI, 49 convent Drive, Bethesda, MD, 20892, USA
299BMutagenesis/RepairOverlapping roles for PSO2 and MSH2-EXO1 in DNA repair.
Peter J. McHugh (1), Louise J. Barber (2), John A. Hartley (1), Thomas A. Ward (1)
(1) Cancer Research UK, WIMM, University of Oxford, Oxford, OX3 9DS, UK; (2) Dept. Oncology, UCL Medical School, London W1W 7BS, UK
300CMutagenesis/RepairInduction of DNA double strand breaks by highly specific endonucleases in Schizosaccharomyces pombe.
Stefan Pellenz (1), Emmanuelle Fabre (1), Bernard Dujon (1), Bernd Schäfer (2)
(1) Unité de Génétique moléculaire des levures (URA 2171/CNRS and UFR927/Univ. P. M. Curie) , Institut Pasteur , 25 Rue du Dr. Roux, F-75724 Paris-CEDEX 15, France; (2) Institut für Biologie IV (Mikrobiologie), Rheinisch Westfälische Technische Hochschule Aachen, Worringer Weg 1, D-52056 Aachen, Germany, Rheinisch Westfälische Technische Hochschule Aachen, D-52056 Aachen, Germany
301AMutagenesis/RepairPartial characterization of Blm10p of Saccharomyces cerevisiae, a nuclear protein that functions in relieving oxidative stress.
Leah D. Pride (1), Kevin Doherty (2), Ajay Pramanik (1), Carol Wood Moore (1)
(1) Microbiology and Immunology, CUNY Medical School, 138th and Convent, New York, NY, 10031, United States; (2) Laboratory of Molecular Gerontology, GRC National Institute on Aging, National Institute of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825
302BMutagenesis/RepairA 131-residue mispair binding domain can be transferred from MSH3 to MSH6 to confer MSH3-like mispair recognition and repair properties onto MSH6.
Scarlet S. Shell, Christopher D. Putnam, Richard D. Kolodner
Ludwig Inst. Cancer Research, Univ. California, San Diego, Mail Code 0669, La Jolla, CA, 92093, USA
303CMutagenesis/RepairMutation induction in budding yeast S.cerevisiae with ion beam irradiation.
Youichirou Matsuo (1), Tomohiro Ishibashi (2), Shigehiro Nishijima (1), Yoshihiro Hase (3), Ayako Sakamoto (3), Atsushi Tanaka (3), Kikuo Shimizu (2)
(1) Department of Nuclear Engineering, Graduate School of Engineering, Osaka University, Yamada oka 2-1, 565-0871, Japan; (2) Radioisotope Research Center, Osaka University, Yamada oka 2-4, Suita, 565-0871, Japan; (3) Department of Ion-beam applied Biology, Japan Atomic Energy Research Institute, Takasaki, Gunma 370-1292, Japan
304AMutagenesis/RepairThe role of LSM1 gene and mRNA turnover in postreplication repair.
Tatana Spicakova, James Brown, Martin Brown
Radiation Oncology, Stanford University, 269 Campus Dr West, Stanford, CA, 94305, USA
305BMutagenesis/RepairEctopic expression of the meiosis-specific CDK like kinase, Ime2, results in genome instability.
Moran Szwarcwort-Cohen, Yona Kassir
Deaprtment of Biology, Technion, Technion City, Haifa, 32000, Israel
306CMutagenesis/RepairThe role of linker domain structure in DNA topoisomerase I function.
Marie Van der Merwe, William C Colley, Andrew Larkin, Mary-Ann Bjornsti
Molecular Pharmacology, St Jude Children's Research, 3320 N Lauderdale St, Memphis, TN, 38105, United states of America
307A
Poster Withdrawn/Cancelled
308B
Poster Withdrawn/Cancelled
309CMutagenesis/RepairYeast as a model to study palindromic gene amplification.
Anne J. Welcker, Alison J. Rattray, Brenda K. Shafer, Jeffrey N. Strathern
GRCBL, NCI-FCRDC, PO Box B, Frederick, MD, 21702, USA
310AMutagenesis/RepairReconstitution of bacterial nonhomologous end joining in yeast: a living test tube to study DNA repair.
Phillip L. Palmbos (1), James M. Daley (1), Leana M. Topper (1), Marina Della (2), Louise M. Tonkin (2), Robert S. Pitcher (2), Aidan J. Doherty (2), Thomas E. Wilson (1)
(1) Department of Pathology, University of Michigan, 1301 Catherine Rd., Ann Arbor, MI, 48109-0602, USA; (2) Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, UK
311BChromosome structureThe role of Pds5p SUMOlation in sister chromatid cohesion.
Gary S. Fortin, Vincent Guacci
Basic Science Division, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA
312CChromosome structureChromatin remodeling activities in DNA damage and repair : What happens at the double strand DNA break site?.
Toyoko Tsukuda, Jac Nickoloff, Mary Ann Osley
Molecular Genetics and Microb, University of New Mexico, 915 Camino de salude, Albuquerque, NM, 87131, USA
313AChromosome structureChromosome specific control of ploidy in the yeast Saccharomyces cerevisiae.
Sanjeev Waghmare, Carlo Bruschi
Microbiology Group, ICGEB, Padriciano,99, Trieste, 34012, Italy
314BChromosome Dynamics: OtherThe effects of chromosome gain: revisiting the tolerance of aneuploidy in yeast.
Kirk Anders, Julie Kudrna
Department of Biology, Gonzaga University, 502 East Boone Ave., Spokane, WA, 99258, USA
315CChromosome Dynamics: OtherGenome-wide synthetic genetic interaction screens with rad53-11 identify regulators and effectors of Rad53.
Mohammed Bellaoui (1), Michael Chang (1), Hong Xu (2), Charles Boone (3), Grant Brown (1)
(1) Biochemistry, University of Toronto, 1 King's College Cir, Toronto, ON, M5S 1A8, Canada; (2) Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON, Canada M5S 1A8; (3) Department of Medical Genetics and Microbiology, and Banting and Best Department of Medical Research University of Toronto, Toronto, ON, Canada M5S 1A8
316AChromosome Dynamics: OtherLocking the DNA topoisomerase I protein clamp: a new Top1p poison.
Komaraiah Palle (1), Michael Woo (2), Hong Guo (2), Piero Benedetti (3), Mary-Ann Bjornsti (2)
(1) St. Jude Children's Research Hospital, Molecular Pharmacology, Memphis TN 38105; (2) Molecular Pharmacology, St. Jude Children's Res Hospit, 332 N Lauderdale, Memphis, TN, 38105, USA; (3) University of Padua, Dept of Biology, Padua, Italy
317BChromosome Dynamics: OtherIsolation and characterization of yeast mutants defective in mitotic chromosome segregation.
Sarah Fatland, Stephanie Leeson, Denisa Bellani, Eric Scolaro, Abby Wood, Chris Young, Heidi Sleister
Department of Biology, Drake University, 2507 University Ave., Des Moines, IA, 50311, USA
318CChromatinSuppression of genomic instability induced by chromatin repressors.
Ritu Agarwal, Jef Boeke
Molecular Biology and Genetics, JohnsHopkins Medical Institute, 725 N Wolfe Street, Baltimore, M, 21205, USA
319AChromatinMutations in SET1 affect ribosomal DNA chromatin structure, transcriptional silencing, and histone H3 methylation in S. cerevisiae.
Catalina Alfonso, Mary Bryk
Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX, 77843-2128, USA
320BChromatinHst3 - an unusual sirtuin, may not require deacetylase activity for its function.
Ivana Celic, Jef Boeke
Molecular Biology and Genetics, Johns Hopkins School of Medici, 725 N. Wolfe Street, Baltimore, MD, 21205, USA
321CChromatinNew silencing proteins identified through a screen for Sir2p interactors.
Russell Darst, Melissa Krick, Sandra N. Garcia, Lorraine Pillus
Div. of Biological Sciences, UC San Diego, 9500 Gilman Dr., La Jolla, CA, 92093, USA
322AChromatinTargeting the chromatin remodeling activity of Isw2 complex in vivo.
Marnie E. Gelbart, Jeff Delrow, Toshio Tsukiyama
Division of Basic Sciences, FHCRC, 1100 Fairview Ave N, Seattle, WA, 98109, USA
323BChromatinMulti-copy suppression of rsc - nhp6 synthetic lethality.
Louis Düring-Olsen, Steen Holmberg
Department of Genetics, University of Copenhagen, O. Farimagsgade 2A, Copenhagen, DK 1353 K, Denmark
324CChromatinNew alleles of SIR2 define cell cycle specific silencing functions.
Scott Holmes, Mirela Matecic, Kristen Martins, Merrit Hickman
Molecular Biology, Wesleyan University, Lawn Avenue, Middletown, CT, 06459, USA
325AChromatinA novel anti-silencing function for the conserved E3 ubiquitin ligase Bre1 distinct from it role in histone H2B monoubiquitination.
William W. Hwang, Shivkumar Venkatasubrahmanyam, Hiten D. Madhani
Department of Biochemistry, UCSF, 600 16th St, San Francisco, CA, 94143-2200, USA
326BChromatinDissection of a complex lethal histone H3 mutant allele that may induce 'death by silencing'.
Edel Hyland, Jef Boeke
Molecular Biology and Genetics, Johns Hopkins University, 725 North Wolfe St., Baltimore, MD, 21205, United States
327CChromatinIdentification of novel factors regulating ubiquitylation of histone H2B.
Cheng-Fu Kao, Nick Clark, Mary Ann Osley
Mol. Genetics & Microbiol., Univ. of New Mexico HSC, 915 Camino de Salud, Albuquerque, NM, 87131, USA
328AChromatinCharacterization of the role of Set1 and COMPASS, a histone H3 methyltransferase complex, in transcriptional silencing at the ribosomal DNA locus of Saccharomyces cerevisiae.
Chonghua Li, John Mueller, Mary Bryk
Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX, 77843-2128, USA
329BChromatinSet1-dependent H3K4 methylation controls promoter proximal pause and release (TEC) of RNA polymerase II in yeast.
Antonin Morillon, Nickoletta Karabetsou, Anitha Nair, Jane Mellor
Dept of Biochemistry, Oxford university, South Parks Road, Oxford, OX13QU, UK
330CChromatinGenome-wide dependence of transcriptional regulation by Rpd3p on the histone H3 and H4 amino termini.
Randall Morse, Nevin Sabet, Sam Volo, Cailin Yu, James Madigan
Developmental Genetics, Wadsworth Center/SUNY Albany, 120 New Scotland Ave, Albany, NY, 12201-2002, USA
331AChromatinInteraction with the lrs domain in sir mediated silencing.
Anne Norris (1), Christopher Fry (2), Michael Cosgrove (3), Craig Peterson (2), Jef Boeke (1)
(1) Molecular Biology and Genetics, Johns Hopkins University, 725 North Wolfe St., Baltimore, MD, 21205, United States; (2) Program in Molecular Medicine, University of Massachusettes Medical School, Worchester, MA; (3) Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD
332BChromatinNovel mutations of the FACT chromatin modulator influence activator-specific transcription.
Allyson O'Donnell (1), Gerald Johnston (2), Richard Singer (1)
(1) Biochemistry & Molecular Biol, Dalhousie University, 5850 College Street, Halifax, NS, B3H 1X5, Canada; (2) Department of Microbiology & Immunology, Dalhousie University, Halifax, NS B3H 1X5, Canada
333CChromatinControl of stochasticity in eukaryotic gene expression.
Jonathan M. Raser, Erin K. O'Shea
Biochemistry and Biophysics, Univ. of Calif., San Francisco, 600 16th Street, San Francisco, CA, 94107, USA
334AChromatinInvestigating the roles of cohesins and condensins on telomeric silencing in yeast.
Tania Rozario, Scott Holmes
MB&B, Wesleyan University, Lawn Ave., Middletown, CT, 06459, USA
335BChromatinFunctional links between acetylation and methylation of chromatin.
Myriam Ruault, Erin M. Scott, Lorraine Pillus
Division of Biology, UCSD, 9500 Gilman Drive, La Jolla, CA, 92093, USA
336CChromatinIdentifying histone structural determinants that specify euchromatin in S. cerevisiae.
Rachel Tompa, Ryan Raisner, Hiten Madhani
Biochemistry and Biophysics, UC San Francisco, 600 16th St, San Francisco, CA, 94143, USA
337AChromatinThe role of RAD6/UBC2 and ubiquitylated H2B in yeast mitosis and meiosis.
Dana Underwood, Mary Ann Osley
Health Sciences Center, University of New Mexico, 915 Camino de Salud, Albuquerque, NM, 87131, USA
338BChromatinInvolvement of histone H1 in heterochromatin barriers.
Marie Veron (1), Eric Gilson (2), Pierre-Antoine Defossez (1)
(1) UMR218, Institut Curie sec. recherche, 26 rue d'Ulm, Paris cedex 05, 75 248, France; (2) LBMC ENS Lyon, 46 allée d'Italie 69 364 Lyon cedex 07, France
339CChromatinStudy of the relationship between yeast Htl1p and RSC complex.
Shan-Li Wang (1), Ali Tsai (2), Mingyuan Cheng (2)
(1) Institute of Genetics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan; (2) Genetics, National Yang-Ming University, Shih-Pai, Taipei, 112, Taiwan
340AChromatinA genetic and biochemical characterization of the SIR2 homolog, HST2.
Jeanne M. Wilson, Collin Zimmerman, Lorraine Pillus
Division of Biological Science, UCSD, 9500 Gilman Dr., La Jolla, CA, 92093-0347, USA
341BChromatinGenome-wide analysis of the histone code.
Amy Martin, Michael Parra, John Wyrick
Molecular Biosciences, Washington State University, Fulmer Hall 675, Pullman, WA, 99164-4660, USA
342CChromatinContribution of the histone H3 N-terminus to global and specific gene expression in the yeast Saccharomyces cerevisiae.
Cailin Yu, Randall Morse
Molecular Genetics Program, Wadsworth Center, 120, New Scotland Av, Albany, NY, 12208, U.S.A.
343AChromatinThe directionality and potency of a silencer are affected by its genomic context.
Yanfei Zou, Qun Yu, Ya-Hui Chiu, Xin Bi
Department of Biology, University of Rochester, Hutchison Hall, Rochester, NY, 14627, USA
344BTranscriptionThe N-terminal arm of Mcm1 affects the transcription of a subset of genes associated with the cell wall.
Deepu S. Abraham, Shira B. Eytan, Andrew K. Vershon
Waksman Institute, Rutgers University, 190 Frelinghuysen Rd, Piscataway, NJ, 08854, USA
345CTranscriptionMechanisms by which p53 binds and remodels chromatin in vivo.
Sri Kripa Balakrishnan, Yoshi Odaka, Geraldine Alba, David S. Gross
Biochemistry & Mol. Biology, LSU health sciences center, 1501 Kings Hwy, Shreveport, LA, 71130, USA
346ATranscriptionPhenotypic analysis of the loss of glucose responsiveness in gcr1-delta cells.
Kellie Barbara (1), Kristine A. Willis (2), George Santangelo (2)
(1) Dept. of Biological Sciences, Univ. of Southern Mississippi, 118 College Dr. 5018, Hattiesburg, MS, 39406, USA; (2) Dept. of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Cir, Toronto M5S 1A8, Canada
347BTranscriptionThe transcription of the monocarboxylic acid transporters JEN1 and ADY2 is regulated by the gene DHH1 in Saccharomyces cerevisiae.
Sónia Barbosa, Sandra Paiva, Margarida Casal
Center/ Dep. of Biology, University of Minho, Campus de Gualtar, Braga, 4710 - 057, Portugal
348CTranscriptionThe transcriptional response of yeast shifted from anaerobic growth to aerobic growth.
Anthony G. Beckhouse (1), Vincent J. Higgins (2), Peter J. Rogers (3), Ian W. Dawes (4)
(1) School of Biotech & Biomol Sci, University of NSW, Kensington, Sydney, NSW, 2052, Australia; (2) School of Science, Food & Horticulture, University of Western Sydney, Locked Bag 1797 DC, South Penrith, NSW, Australia, 1797; (3) Carlton & United Breweries Ltd, 4-6 Southampton Crescent, Abbotsford, VIC, Australia, 3067; (4) Clive and Vera Ramaciotti Centre for Gene Function Analysis, University of NSW, Sydney, NSW, Australia, 2052
349ATranscriptionRegulation of transcriptional repression by Nrg1 and Nrg2.
Cristin D. Berkey (1), Valmik K. Vyas (2), Marian Carlson (3)
(1) Genetics and Development, Columbia University, 701 West 168 Street , New York, NY 10032, USA; (2) Integrated Program in Cellular, Molecular and Biophysical Studies, Columbia University, 701 West 168 Street, New York, NY 10032, USA; (3) Genetics and Development, Columbia University, 701 West 168 Street, New York, NY, 10032, USA
350BTranscriptionOver-expression of Cln2 or Cln3 suppresses loss of the Paf1/RNA polymerase II complex.
Joan L. Betz (1), Bao T. Nguyen (1), Judith A. Jaehning (2)
(1) Biology, Regis University, 3333 Regis Blvd., Denver, CO, 80221, USA; (2) Department of Biochemistry and Molecular Genetics, University of Colorado HSC, Denver
351CTranscriptionNuclear-dependent degradation of Msn2 attenuates the general stress response of Saccharomyces cerevisiae.
Sohini Bose, Richard S. Zitomer
Dept. of Biological Sciences, University at Albany/SUNY, 1400 Washington Ave., Albany, NY, 12222, USA
352ATranscriptionMutational analysis of S. cerevisiae TFIIF: functional divergence between S. pombe and S. cerevisiae and identification of mutants conferring upstream shifts in transcription start site selection.
Seth A. Brodie, Mohamed A. Ghazy, Alfred S. Ponticelli
Department of Biochemistry, University at Buffalo, 3545 Main St., Buffalo, NY, 14214, United States
353BTranscriptionTranscription of the yeast iron regulon responds not directly to iron but to iron-sulfur cluster biosynthesis.
Opal Chen (1), Robert Crisp (1), Martin Valachovic (2), Martin Bard (3), Dennis Winge (4), Jerry Kaplan (1)
(1) Department of Pathology, University of Utah, 30 N. Medical Dr, Salt Lake City, UT, 84132, USA; (2) Department of Biology, Indiana University-Prudue University Indianapolis, Indiana; (3) Department of Biology, Indiana University-Prudue University, Indianapolis, Indiana; (4) Departments of Medicine and Biochemistry, University of Utah, Salt Lake City, Utah
354CTranscriptionMutational analysis of the pol II rDNA promoter in S. cerevisiae.
Parmeet Jodhka, Arland Alberts, Matt Haymowicz, Gunisha Sagar, Heather Conrad-Webb
Biology, Texas Woman's University, PO Box 425799, Denton, TX, 76204, USA
355ATranscriptionGal3-Gal80 association is mediated by interaction between Gal3's N- and C-termini: a conformational target for ATP and galactose.
Cuong Diep, Gang Peng, Vepkhia Pilauri, Tamara Vyshkina, Paul Darminio, James Hopper
Biochemistry & Molecular Biol., Penn State College of Med, 500 University Dr., Hershey, Pa, 17033, USA
356BTranscriptionElements involved in cadmium sensitivity at KlHIS4 promoter.
Mónica Lamas-Maceiras (1), Manuel Becerra (2), Silvia Seoane (2), María A. Freire-Picos (2)
(1) Area de Microbiología. Fac. Ciencias Biológicas y ambientales., Univ. de León. 24071 León, Spain; (2) Biología Celular y Molecular, Universidade da Coruña, Campus da Zapateira, A Coruña, 15071, Spain
357CTranscriptionDissecting complex transcriptional responses to single stimuli.
Cristy L. Gelling (1), Johnny Lee (1), Geoffrey D. Kornfeld (2), Ian W. Dawes (2)
(1) School of Biotech & Biomol Sci, University of NSW, Kensington, Sydney, 2052, Australia; (2) Ramaciotti Centre for Gene Function Analysis and School of Biotech & Biomol Sci, University of NSW, Kensington, Sydney, 2052, Australia
358ATranscriptionGenetic interaction between the uncharacterized ORF YER139C and RNA polymerase II core subunits RPB5 and RPB9.
Kevin Morano, Amy Trott, Patrick Gibney, Reginald Hence, Carmen Galvan
Dept. Microbiol. Mol. Genet., Univ. Texas Med. Schl. Houston, 6431 Fannin St., Houston, TX, 77030, USA
359BTranscriptionRole of phosphatidylcholine (PC) turnover products in regulation of the INO1 gene: Sec14 homologues study.
Roman Holic (1), Peter Griac (1)
(1) Bioenergetics, Animal Bioch. and Genetics, Moyzesova 61, Ivanka pri Dunaji, 900 28, Slovakia
360CTranscriptionContext-dependent oxidative stress inducibility of Yap1p-responsive genes.
Kailash Gulshan, Sherry Rovinsky, Scott Moye-Rowley
Physiology and Biophysics, University of Iowa, 51 Newton Road, Iowa City, IA, 52242, United States
361ATranscriptionThe Srb mediator subunit Gal11p and TFIIE are required for Gcn4p function in vivo.
Jia Guo, Caroah Sykes, Jennifer Paris, Mark J. Swanson
School of Biological Sciences, Louisiana Tech University, 1 Adams Blvd, Ruston, LA, 71272, USA
362BTranscriptionScreen for Histone H3 mutants that de-repress the Saccharomyces cerevisiae CHA1 gene under non-inducing conditions.
Qiye He (1), Randal Morse (2)
(1) Department of Biomedical Sciences,Wadsworth Center and SUNY, Albany; (2) Biomedical Sciences, Wadsworth Center, SUNY, Albany, 150 New Scotland Av., Albany, NY, 12208, Unite States of America
363CTranscriptionSite-specific genomic (SSG) and random domain-localized (RDL) mutagenesis in yeast.
Misa Gray, Sarah Piccirillo, Saul M. Honigberg
Div. Cell Biology & Biophysics, University of Missouri-KC, 5100 Rockhill Rd, Kansas City, MO, 64110-2499, USA
364ATranscriptionMsi1p/Cac3p activates transcription only in the absence of fermentation.
Zachary L. Pratt, Devin C. Miller, Stephen D. Johnston
Department of Biology, North Central College, 30 N. Brainard St., Naperville, IL, 60540, USA
365BTranscriptionMutations in septins cause constitutive glucose derepression.
Nataly Kacherovsky, Valentina Voronkova, Diana Yu, Chris Tachibana, Elton T. Young
Biochemistry, University of Washington, Box 357350, Seattle, WA, 98195-7350, United States
366CTranscriptionGenetic interactions between TAF14p and TFIIS.
Rachel N. Fish (1), Judith K. Davie (2), Cindy Pham (1), Lynn M. Ziegler (3), Betty F. Lu (4), Alfred S. Ponticelli (3), Caroline M. Kane (1)
(1) Molecular and Cell Biology, Univ. of California, Berkeley, 408 Barker Hall, Berkeley, CA, 94720-3202, United States; (2) Biochemistry/Molecular Biology, University of Texas, M. D. Anderson Cancer Center, Houston TX 77030; (3) Department of Biochemistry, SUNY Buffalo, Buffalo NY 14214-3000; (4) Department of Immunology, Washington University, St. Louis MO
367ATranscriptionAnalysis of heat shock factor (HSF1) and its in vivo binding partners in Saccharomyces cerevisiae.
Selena B. Kremer, David S. Gross
Biochemistry and Mol. Biology, LSUHSC-Shreveport, 1501 Kings Hwy, Shreveport, LA, 71130, USA
368BTranscriptionMaf1 mediated regulation of RNA polymerase III transcription.
Danuta Oficjalska (1), Olivier Harismendy (1), Wieslaw Smagowicz (2), Magdalena Boguta (2), André Sentenac (1), Olivier Lefebvre (1)
(1) DBJC/SBGM, CEA/Saclay, Batiment 144, GIf-Sur-Yvette, 91191, France; (2) Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02 106 Warsaw, Poland
369CTranscriptionPoster Award: 1st Place  Analysis of intergenic transcription as a regulator of gene expression in S. cerevisiae.
Joseph A Martens, Lisa Laprade, Fred Winston
Genetics, Harvard Medical School, 77 Ave Louis Pasteur, Boston, MA, 02115, USA
370ATranscriptionThe requirements for induction of the hypoxic genes in Saccharomyces cerevisiae.
Thomas A. Mennella, Richard S. Zitomer
Dept. of Biological Sciences, University at Albany/SUNY, 1400 Washington Ave, Albany, NY, 12222, USA
371BTranscriptionThe role of SAGA in ADE gene expression in Saccharomyces cerevisiae.
Rebecca Mitsch, Jennifer Urbanowski, Ronda Rolfes
Department of Biology, Georgetown University, 37th and O Street NW, Washington, DC, 20057-1229, USA
372CTranscriptionDomain characterization of Pdc2.
Dominik Mojzita (1), Stefan Hohmann (2)
(1) Department of Cell and Molecular Biology/Microbiology, Medicinaregatan 9C, S-413 30 Göteborg, SWEDEN; (2) CMB/Microbiology, Gothenburg University, Medicinaregatan 9C, Gothenburg, 41390, Sweden
373ATranscriptionCharacterization of TAR1 Regulation and Protein Function.
Janine A. Mok (1), Paulo S.R. Coelho (2), Anthony C. Bryan (3), Anuj Kumar (4), Gerald S. Shadel (3), Michael P. Snyder (5)
(1) Department of Genetics, Yale University, P.O. Box 208103, New Haven, CT, 06520, USA; (2) Universidade Sai Paulo, Faculdade de Medicina de Ribeirao Preto, Departamento de Biologia Celular, Molecular, e Bioagentes Patogenicos, Av. Bandeirantes 3900, 14049-900, Ribeirao Preto, SP - Brasil; (3) Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Rollins Research Center, Atlanta, GA 30322; (4) Life Science Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109; (5) Department of Molecular, Cellular and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520
374BTranscriptionPhosphorylation of the Ctk1p T-loop es essential for its kinase activity.
Denis Ostapenko, Mark Solomon
Molecular Biophysics & Biochem, Yale University, 333 Cedar St, New Haven, C, 06520, US
375CTranscriptionRetrograde regulation of the multidrug transporter gene PDR5 requires Lge1p, a multifunctional component of the histone H2B ubiquitinating complex.
Xiaoting Zhang (1), Anna Kolaczkowska (1), Frederic Devaux (2), Sneh Panwar (1), Heather Scoville (1), Timothy Hallstrom (1), Claude Jacq (2), Scott Moye-Rowley (1)
(1) Physiology and Biophysics, University of Iowa, 51 Newton Road, Iowa City, IA, 52242, USA; (2) Laboratoire de Genetique Moleculaire, CNRS, Paris, France 75320
376ATranscriptionStructural and functional relationship between nuclear pore complexes and the global transcriptional activators Gcr1 and Gcr2.
Satish Pasula (1), Balaraj B. Menon (1), Nayan J. Sarma (1), Kristine A. Willis (2), George Santangelo (2)
(1) Dept. of Biological Sciences, Univ. of Southern Mississippi, 118 College Dr. 5018, Hattiesburg, MS, 39406, USA; (2) Dept. of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Cir, Toronto M5S 1A8, Canada
377BTranscriptionIdentification and characterization of Elf1, a novel transcription elongation factor in S. cerevisiae.
Donald Prather, Fred Winston
Department of Genetics, Harvard University, 77 Ave Louis Pasteur, Boston, MA, 02115, USA
378CTranscriptionAn array of coactivators is required for optimal recruitment of TBP and RNA Polymerase II by promoter-bound Gcn4p.
Hongfang Qiu (1), Cuihua Hu (1), Sungpil Yoon (1), Krishnamurthy Natarajan (2), Mark J. Swanson (3), Alan G. Hinnebusch (1)
(1) LGRD, NICHD, 6 Center Dr. , Bldg 6A, Rm B1A-13, Bethesda, MD 20892; (2) School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India; (3) Carson Taylor Hall, Room 121, School of Biological Sciences Louisiana Tech University, 1 Arizona, POB 3179, MC 37, Ruston, LA 71272-3045
379AGenomicsPoster Award: 3rd Place  The transcriptional regulation of entry and exit from stationary phase in S. cerevisiae.
Marijana Radonjic, Jean-Christophe Andrau, Patrick P.C.W. Kemmeren, Philip Lijnzaad, Dik van Leenen, Frank C.P. Holstege
Genomics Laboratory, UMC Utrecht, Universiteitsweg 100, Utrecht, 3584 CG, The Netherlands
380BTranscriptionMeiosis-specific transcriptional regulation of the Saccharomyces cerevisiae S-phase cyclin CLB5.
Sheetal Raithatha, Catherine Hui, David Stuart
Biochemistry, University of Alberta, 561 Med Sci Building, Edmonton, AB, T6G 2H7, Canada
381CTranscriptionFunctional interaction between RNAP II and the Ssu72 CTD phosphatase.
Mariela Reyes-Reyes, Michael Hampsey
Biochemistry, RWJMS-GSBS, 675 Hoes Lane, Piscataway, NJ, 08854, USA
382ATranscriptionFeedback regulation of ergosterol biosynthesis through the transcription factors Upc2p and Ecm22p.
Brandon Davies (1), John Miller (2), Stan Fields (2), Jasper Rine (1)
(1) Molecular and Cell Biology, Univ. of California-Berkeley, 522 Barker Hall, Berkeley, CA, 94720, USA; (2) Department of Genome Sciences, University of Washington, Box 357730, Seattle, WA 98195-7730
383BTranscriptionTranscriptional regulation of TUP1 gene in Kluyveromyces lactis.
Ana Rodriguez-Torres
Biologia Celular y Molecular, Universidade da Coruna, Campus da Zapateira, A Coruna, 15071, Spain
384CTranscriptionRole of phospholipase C in transcriptional regulation.
Carlos Romero, Peter Nguyen, Nilanjan Guha, Agnieszka Demczuk, Parima Desai, Ales Vancura
Biological Sciences, St. John's University, 8000 Utopia Parkway, Jamaica, NY, 11439, USA
385ATranscriptionNew Factors in the Transcription of Ribosomal Protein Genes.
Dipayan Rudra, Jonathan R. Warner
Cell Biology, Albert Einstein College of Med, 1300 Morris Park Av, Bronx, NY, 10461, United States of America
386BTranscriptionReverse recruitment: is nuclear substructure an essential feature of eukaryotic transcriptional activation?.
Nayan J. Sarma (1), Satish Pasula (1), Balaraj B. Menon (1), George Santangelo (2)
(1) Dept. of Biological Sciences, Univ. of Southern Mississippi, 118 College Dr. 5018, Hattiesburg, MS, 39406, USA; (2) Dept. of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Cir, Toronto M5S 1A8, Canada
387CTranscriptionModulation of transcription factor function by an amino acid: Activation of Saccharomyces cerevisiae Put3p by proline.
Christopher A. Sellick, Richard J. Reece
School of Biological Sciences, The University of Manchester, Oxford Road, Manchester, M13 9PT, United Kingdom
388ATranscriptionMechanism of regulation of anaerobic genes in Saccharomyceds cerevisiae.
Sertil Odeniel, Arvind Vemula, Sharon Salmon, Charles Lowry
Immunol Microbial Disease, Albany Medical College, 47 New Scotland Ave, Albany, NY, 12208, USA
389BTranscriptionDNA-bound Bas1 recruits Pho2 to promoters to stimulate expression of the ADE genes in Saccharomyces cerevisiae.
Indrani Som, Jennifer Urbanowski, Ronda Rolfes
Department of Biology, Georgetown University, 37th and O Street NW, Washington, DC, 20057-1229, USA
390CTranscriptionRegulation of TBP-TFIIA binding by chromatin regulatory factors.
Debabrata Biswas, Peter Eriksson, Yaxin Yu, David Stillman
Pathology, University of Utah, 50 N. Medical Drive, Salt Lake City, UT, 84132, United States
391ATranscriptionRecruitment of a triad of subunits from the Gal11/tail domain of Srb mediator supports transcriptional activation by Gcn4p in vivo.
Fan Zhang (1), Laarni Sumibcay (1), Alan G. Hinnebusch (1), Mark J. Swanson (2)
(1) Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892; (2) School of Biological Sciences, Louisiana Tech University, 1 Adams Blvd, Ruston, LA, 71272, USA
392BTranscriptionDNA binding as a mechanism of combinatorial control.
Chris Tachibana (1), Tong I. Lee (2), Jane Y. Yoo (2), Jean-Bosco Tagne (2), Nataly Kacherovsky (1), Richard A. Young (2), Elton T. Young (1)
(1) Biochemistry, University of Washington, Box 357350, Seattle, WA, 98195, USA; (2) Whitehead Institute, 9 Cambridge Center, Cambridge MA
393CTranscriptionComplex interplay among regulators of drug resistance in S. cerevisiae.
Bernard Turcotte, Bassel Akache, Sarah MacPherson, Marc-André Sylvain
Dept. Medicine, McGill University, 687 Pine Ave. West, Montréal, QC, H3A 1A1, Canada
394ATranscriptionRole of the N-terminal region of Rap1p in the transcriptional activation of glycolytic genes in Saccharomyces cerevisiae.
Hiroshi Uemura (1), Takayuki Mizuno (1), Tomoko Kishimoto (1), Tomoko Shinzato (1), Robin Haw (1), Alistair Chambers (2)
(1) Inst. Biol. Resources and Func, AIST, Higashi 1-1-1, Tsukuba, 305-8566, Japan; (2) Inst. of Genetics, Univ. of Nottingham, Queen's Medical Centre, Nottingham, NG7 2UH, UK
395BTranscriptionThe SAGA complex represses the transcription of the SER3 gene.
Pei-Yun Jenny Wu, Joseph A. Martens, Fred Winston
Genetics, Harvard Medical School, 77 Ave Louis Pasteur, Boston, MA, 02115, USA
396CTranscriptionFunctions of protein factors involved in ribosomal protein gene transcription.
Yu Zhao, Jonathan R. Warner
Cell Biology, Albert Einstein College of Med, 1300 Morris Park Ave, Bronx, NY, 10461, USA
397ARNA processingCharacterization of the pre-rRNA processing factor Rrp9p and Sof1p in yeast.
Ralph Bax, Chris Vos, Dick Raué
Biochemistry, Vrije Universiteit, De Boelelaan 1083, Amsterdam, 1081 HV, The Netherlands
398BRNA processingProbing the role of RNA helicases in large ribosomal subunit biogenesis.
Kara A. Bernstein (1), Sander Granneman (2), Alicia Lee (2), Susan J. Baserga (3)
(1) Genetics, Yale School of Medicine, 333 Cedar Street, New Haven, CT, 06520, USA; (2) Molecular Biochemistry and Biophysics, 333 Cedar Street, New Haven, CT 06520; (3) Genetics, Therapeutic Radiology, Molecular Biochemistry and Biophysics, 333 Cedar Street, New Haven, CT 06520
399CRNA processingBinding of the nucleolar protein Nep1 to a specific site in ribosomal RNA and isolation of deltasnr57 and multicopy RPS19 as nep1 suppressors.
Markus Buchhaupt, Britta Meyer, Peter Kötter, Karl-Dieter Entian
Institute of Microbiology, JW-Goethe University, Marie-Curie-Str. 9, Frankfurt am Main, 60439, Germany
400ARNA processingYeast Rrp5p, a trans-acting factor involved in both 40S and 60S subunit biogenesis, is an RNA-binding protein with a strong preference for U-rich sequences.
Paulo de Boer, Harmjan Vos, Alex Faber, Chris Vos, Hendrik Raué
Biochemistry and Mol Biology, Vrije Universiteit Amsterdam, de Boelelaan 1083, Amsterdam, 1081 HV, The Netherlands
401BRNA processingInactivation of Rex4p or Rnt1p Redirects Pre-rRNA Processing in Saccharomyces cerevisiae.
Alex Faber (1), Chris Vos (1), Harmjan Vos (1), Ghada Ghazal (2), Sherif Abou Elela (2), Hendrik Raué (1)
(1) Biochemistry and Mol Biology, Vrije Universiteit Amsterdam, De Boelelaan 1083, Amsterdam, 1081 HV, The Netherlands; (2) Groupe ARN/RNA Group, Département de Microbiologie et d'Infectiologie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4
402CRNA processing5-Fluorouracil enhances exosome-dependent accumulation of polyadenylated rRNAs.
Feng Fang, Jason Hoskins, Scott Butler
Microbiology and Immunology, University of Rochester, 601 Elmwood Ave, Rochester, NY, 14642, USA
403ARNA processingA global view of yeast noncoding RNA processing and modification.
Shawna Hiley, Miles Trochesset, Quaid Morris, Tomas Babak, Timothy Hughes
BBDMR, University of Toronto, 112 College St., Toronto, ON, M4T 2J4, Canada
404BRNA processingPoster Award: Honorable Mention  Splicing: A new level of regulation in Saccharomyces cerevisiae.
Linda J. Palmisano (1), Cristy L. Gelling (1), Geoffrey Kornfeld (2), Ian W. Dawes (2)
(1) School of Biotech & Biomol Sci, University of New South Wales, Sydney, New South Wales, 2052, Australia; (2) Ramaciotti Centre for Gene Function Analysis and School of Biotech & Biomol Sci, University of NSW, Sydney, NSW, 2052, Australia
405CRNA processingGuaranteeing tRNA's function: systematic genetic analysis identifies pus4 cellular interactions.
David E. W. Arnolds, Wendy E. Raymond
Department of Biology, Williams College, 59 Lab Campus Drive, Williamstown, MA, 01267, USA
406ARNA processingCharacterization of Nme2p: a unique protein component of RNase MRP.
Kelly Salinas, Sara Wierzbicki, Li Zhou, Mark E. Schmitt
Biochemistry, Upstate Medical University, 750 E. Adams St, Syracuse, NY, 13210, USA
407BRNA processingSbp1p and Tat1p act as multi-copy suppressors of temperature sensitive tyrosyl-tRNA synthetase (tys1-1).
Michael Whitney, Abul Azad, Rebecca Hurto, Anita K. Hopper
Biochemistry and Mol. Biol., Penn State College of Medicine, 500 University Dr, Hershey, PA, 17033, USA
408CRNA turnoverMultiple Transcripts Regulate Saccharomyces cerevisiae JEN1 mRNA Glucose-Triggered Decay.
Raquel Andrade (1), Peter Kötter (2), Karl-Dieter Entian (2), Margarida Casal (3)
(1) Center/Dep. of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal / ICVS, Escola de Ciências da Saúde, U. Minho, 4710-057 Braga, Portugal; (2) Institut für Mikrobiologie, Johann Wolfgang Goethe-Universität Frankfurt, 60439 Frankfurt, Germany; (3) Center/Dep. of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal
409ANucleo/cytoplasmic transportNuclear pore complex function is influenced by glycosylation in Saccharomyces cerevisiae.
Kristy MacDonald, Christina Ott, Laura Davis, Kenneth Belanger
Biology, Colgate University, 13 Oak Dr, Hamilton, NY, 13346, USA
410BNucleo/cytoplasmic transportThe role of integral membrane proteins and the secretory pathway in NPC assembly and morphology.
Alexis Madrid, Karsten Weis
Molecular and Cell Biology, UC Berkeley, 329 LSA, Berkeley, CA, 94720, US
411CNucleo/cytoplasmic transportThe N-terminal domain of the yeast hnRNP, Nab2p, makes critical protein interactions necesarry for proper mRNA and hnRNP export.
Maja Ordanic-Kodani, Deanna Green, Kavita Marfatia, Christie Johnson, Allison Lange, Henry Hagan, Anita Corbett
Biochemistry, Emory University, 1510 Clifton Rd., Atlanta, GA, 30322, United States
412ANucleo/cytoplasmic transportNuclear/Cytosolic Dynamics of Yeast C, C, and A Adding Enzyme, Cca1p.
Hussam Shaheen, Anita Hopper
Biochem. and Molec. Biol, Pennstate College of Med, 500 University Dr, Hershey, PA, 17033, USA
413BNucleo/cytoplasmic transportSystematic analysis of overlapping functions of the Importin-beta family.
Michael Whitney, Anita K. Hopper
Biochem. and Mol. Biol., Penn State College of Medicine, 500 University Dr., Hershey, PA, 17033, USA
414CNucleo/cytoplasmic transportThe FG-repeat asymmetry of the yeast nuclear pore complex is dispensable for bulk nucleocytoplasmic transport in vivo.
Bryan Zeitler, Karsten Weis
Molecular and Cell Biology, UC Berkeley, LSA 329, Berkeley, CA, 94720, USA
415AEpigenetic mechanismsSpecies barrier in URE3 prion transmission : from S.cerevisiae to S.uvarum and S.paradoxus.
Nicolas Talarek, Christophe Cullin, Michel Aigle
IBGC, CNRS/Universite Bordeaux2, 1, rue C.Saint Saens, Bordeaux, 33077, FRANCE
416BEpigenetic mechanismsFormation of boundaries of transcriptionally silent chromatin by nucleosome-excluding structures.
Xin Bi, Qun Yu, Joe Sandmeier, Yanfei Zou
Biology, University of Rochester, 307 Hutchison Hall, Rochester, NY, 14627, USA
417CEpigenetic mechanismsAnalysis of amyloid formation in Saccharomyces cerevisiae.
Andreas Brachmann, Reed B. Wickner
Lab. of Biochem. and Genetics, NIDDK, NIH, 8 Center Drive, Bethesda, MD, 20892-0830, USA
418AEpigenetic mechanismsA genomic method for identification of structures in yeast which are incapable of self-assembly.
Daniel Lockshon, Brian Kennedy
Department of Biochemistry, University of Washington, Box 357350, Seattle, WA, 98195, United States
419BEpigenetic mechanismsThe cell cycle requirement for silencing establishment.
Marc Meneghini, Hiten Madhani
Biochemistry and Biophysics, UCSF, 600 16th St, San Francisco, CA, 94143-2200, U.S.A.
420CEpigenetic mechanismsEvaluation of Pin3, a putative prion in Saccharomyces cerevisiae.
Andrew O'Dell, Susan Liebman
Lab for Molecular Biology, U of Illinois at Chicago, 900 S Ashland Ave, Chicago, IL, 60607, USA
421A
Poster Withdrawn/Cancelled
422BEpigenetic mechanismsMolecular mechanisms of [PSI+] induced phenotypic variation in yeast.
Marenda A. Wilson (1), Stacie Meaux (1), Roy Parker (2), Ambro van Hoof (1)
(1) Microbiol. and Mol. Genet., UTHSC, 6431 fannin, Houston, TX, 77030, USA; (2) HHMI / University of Arizona, Mol. Cell. Biol., Tucson AZ 85721 USA
423CTranslationLAS24 is involved in local anesthetic drug resistance and regulates translation initiation in responding to the TOR and PKA signaling pathway.
Tomoyuki Araki, Yukifumi Uesono, Tomoko Oguchi, Akio Toh-e
Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Tokyo, 113-0033, Japan
424ATranslationPropagation of L-A virus in the yeast Saccharomyces cerevisiae cells depleted of the ribosomal stalk proteins.
Dawid Krokowski, Marek Tchorzewski, Alaksandra Boguszewska, Dariusz Abramczyk, Nikodem Grankowski
Dep. Molecular Biology, MC Sklodowska University, Akademicka 19, Lublin, 20-033, POLAND
425BTranslationCharacterization of yeast Saccharomyces cerevisiae N-terminal protein acetylatransferases.
Bogdan Polevoda, Steven Brown, Fred Sherman
Biochemistry and Biophysics, University of Rochester, 601 Elmwood Ave., Rochester, NY, 14642, USA
426CTranslationPotential roles for Yar1 and Ltv1 proteins in ribosome biogenesis.
Robert M. Seiser (1), Jesse W. Loar (2), Deborah E. Lycan (2)
(1) Biochemistry Department, UW-Madison, 433 Babcock Dr., Madison, WI, 53706, USA; (2) Biology Department, Lewis and Clark College, Portland, OR 97219, USA
427ATranslationRole of Rck2 in global transcriptional and translational regulation upon oxidative stress.
Swarnalatha Swaminathan (1), Tomas Masek (2), Martin Pospisek (2), Per Sunnerhagen (1)
(1) Molecular Biology, Gothenburg University, Medicinaregatan 9C, Gothenburg, 413 26, Sweden; (2) Genetics and Microbiology, Faculty of Science, Vinicna 5, Prague, 128 44, Czech Republic.
428BTranslationMapping eIF5A binding sites for Dys1 and Lia1: in vivo evidence for regulation of eIF5A hypusination.
Gloria M. Thompson, Veridiana S.P. Cano, Sandro R. Valentini
Biological Sciences, São Paulo State University, Rod. Araraquara-Jau, Araraquara, SP, 14801-902, Brazil
429CTranslationComparative and functional study of micro open reading frames (uORF) in the 5' untranslated regions (UTR) of genes in Saccharomyces cerevisiae.
Zhihong Zhang, Fred Dietrich
MGM, Duke University Medical Center, Box 3568 DUMC, Durham, NC, 27710, USA
430AGene Expression: OtherSaccharomyces cerevisiae as a host for heterologous gene expression.
Gia C. Fazio, Seiichi P.T. Matsuda
Biochemistry and Cell Biology, Rice University, 6100 Main St., Houston, TX, 77005, USA
431BGene Expression: OtherProduction of isoprenoids in Saccharomyces cerevisiae.
James Kirby, Dante W. Romanini, Jay D. Keasling
Dept. of Chemical Engineering, UC Berkeley, 401 Latimer Hall, Berkeley, CA, 94720, USA
432CGene Expression: OtherA possible role of TFIIB under non-physiological conditions.
Joana Monteiro (1), Lisete Fernandes (2)
(1) Instituto Gulbenkian de Ciência, Oeiras, Portugal; (2) Instituto Gulbenkian de Ciência, Oeiras, Portugal. Escola Superior de Tecnologia da Saúde de Lisboa, Lisbon, Portugal
433AGene Expression: OtherPurification of the Cucumber necrosis virus replicase from yeast: the role of co-expressed viral RNA in stimulation of replicase activity.
Zivile Panaviene, Peter D. Nagy
Plant Pathology Department, University of Kentucky, 1405 Veterans Dr., Lexington, KY, 40546, USA
434BGene Expression: OtherMetabolic Engineering of Saccharomyces cerevisiae for the increased production of isoprenoids.
Eric Paradise, James Kirby, Sydnor Withers, Jay Keasling
Chemical Engineering, UC Berkeley, 401 Latimer Hall, Berkeley, CA, 94720-1462, United States
435CGene Expression: OtherFunctional analysis of a conserved domain located between helices 7 and 8 of the lactate permease Jen1p in Saccharomyces cerevisiae.
Isabel Soares-Silva (1), George Diallinas (2), Margarida Casal (1)
(1) Center/Dep. of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal; (2) Faculty of Biology, Department of Botany, University of Athens, Panepistimioupolis, Athens 15781, Greece
436AGene Expression: OtherResistance to the antimalarial drug artesunate involves the Pdr1p-mediated activation of TPO1 and PDR5 expression.
Sandra Tenreiro, Marta Alenquer, Isabel Sá-Correia
Biolog. Scienc. Research Group, Instituto Superior Técnico, Av. Rovisco Pais, Lisbon, 1049-001, Portugal
437BGenomicsGlobal genome mapping in wild yeast : from acetate production to the catalytic core of asparaginaseI in oenology.
Philippe Marullo (1), Gael Yvert (2), Marina Bely (1), Pascal Durrens (3), Isabelle Masneuf-Pomarede (1), Denis Dubourdieu (1), Michel Aigle (4)
(1) Faculte d'oenologie, Universite Bordeaux2., 33405 TALENCE FRANCE; (2) INSA, 135 Av. RANGUEIL, 31000 TOULOUSE, FRANCE; (3) IBGC.CNRS.Universite Bordeaux2, 1 rue C. Saint Saens, 33077 Bordeaux FRANCE; (4) IBGC, CNRS/Universite Bordeaux2, Camille Saint Saens, BORDEAUX, 33077, FRANCE
438CGenomicsGenome-wide suppressor analysis of yeast and human genes with Saccharomyces nonessential deletion mutants.
Rinji Akada, Takao Kitagawa, Yuji Torigoe, Koji Ueno, Yoshito Kakihara, Hisashi Hoshida
Dept Appl Chem & Chem Eng, Yamaguchi Univ, Fac Eng, Tokiwadai 2-16-1, Ube, 755-8611, Japan
439AGenomicsPoster Award: Honorable Mention  Yeast genomics in the classroom: identification of S. cerevisiae deletion strains that are hypersensitive to SDS, NaF and high temperature.
Anna Ballew, Bio 55 Students, Martha Cyert, Tim Stearns
Biological Sciences, Stanford University, 337 Campus Drive, Stanford, CA, 94305, USA
440BGenomicsFunctional genomics of monensin sensitivity in yeast.
Gunilla Barmark (1), Marie Gustavsson (2), Jimmy Larsson (3), Eva Murén (3), Hans Ronne (3)
(1) Dep of Plant Biol & Forest Gen, Swedish Univ Agric Sciences, Box 7080, Uppsala, S-750 07, Sweden; (2) Department of Medical Biochemistry and Microbiology, Uppsala University, Box 582, SE-75123 Uppsala, Sweden; (3) Department of Plant Biol. & Forest Genetics, SLU, Box 7080, SE-75007 Uppsala, Sweden AND Department of Medical Biochemistry and Microbiology, Uppsala University, Box 582, SE-75123 Uppsala, Sweden
441CGenomicsQTLs controlling sporulation efficiency in budding yeast.
Giora Ben-Ari (1), Drora Zenvirth (2), Amir Sherman (3), Uri Lavi (3), Jossi Hillel (1), Giora Simchen (2)
(1) Plant Sciences & Genetics, Hebrew University of Jerusalem, Hertzel, Rehovot, 76100, Israel; (2) Department of Genetics, The Hebrew University of Jerusalem, Givat-Ram Campus, Jerusalem 91904, Israel; (3) ARO, Volcani Center, P.O Box 6, Bet Dagan, Israel
442AGenomicsIntegration of chemogenomic and transcriptional profiles to characterize the cellular response to the antifolates Methotrexate and Sulfanilamide in yeast.
Javier Botet, Montserrat Martin, Jose Luis Revuelta
Microbiologia y Genetica, Universidad de Salamanca/CSIC, Doctores de la Reina, Salamanca, 37007, Spain
443BGenomicsThe Yeast Deletion Project v.2.0.
Angela Chu (1), Li Ni (2), Hui Yang (2), Lucy Liu (2), Adam Deutschbauer (1), Guri Giaever (3), Daniel Richards (1), Jef Boeke (4), Micheal Snyder (2), Ronald Davis (3)
(1) Biochemistry, Stanford University, 318 Campus Dr., Stanford, CA, 94305, USA; (2) Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520; (3) Stanford Genome Technology Center, 855 California Avenue, Palo Alto, California, 94304; (4) Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, Baltimore MD 21205
444CGenomicsMapping genetic interaction networks among essential genes via titratable promoter alleles.
Armaity Davierwala (1), Jennifer Haynes (2), Zhijian Li (1), Renee Brost (1), Amy Tong (1), Sanie Mnaimneh (1), Brenda Andrews (2), Charlie Boone (1), Timothy Hughes (1)
(1) BBMDR, University of Toronto, 112 College Street, Toronto, ON, M5G 1L6, Canada; (2) Dept. of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Toronto ON M5S 1A8, Canada
445AGenomicsExpanding the role of Reciprocal Hemizygosity Scanning (RHS).
Jed Dean (1), John McCusker (2), Ron Davis (1), Lars Steinmetz (3)
(1) Biochemistry, Stanford University, 855 California, Palo Alto, CA, 94304, USA; (2) Duke University; (3) EMBL
446BGenomicsMapping the genetic determinants of yeast sporulation efficiency: a quantitative trait.
Adam Deutschbauer (1), Ronald W. Davis (2)
(1) Department of Biochemistry, Stanford University, 318 Campus Drive, Stanford, CA 94305; (2) Department of Biochemistry, Stanford University, 318 Campus Drive, Stanford, CA, 94305, USA
447CGenomicsDifferential expression of genes in enological strains of Saccharomyces cerevisiae manifest in differences in osmo-sensitivity, acetic acid and glycerol formation during sugar induced osmotic stress.
Daniel J. Erasmus, Hennie J. J. van Vuuren
Wine Research Centre, University of British Columbia, 2205 East Mall, Vancouver, BC, V6T 1Z4, Canada
448AGenomicsChemical functional genomics of fungal lipid and membrane homeostasis.
Daniel Chirinos, Virginia Aberdeen, Kangze He, Annapurna Dhanekula, William Starmer, Scott Erdman
Department of Biology, Syracuse University, 130 College Place, Syracuse, NY, 13244-1220, USA
449BGenomicsA genome-wide survey of haploinsufficiency in yeast.
Adam M. Deutschbauer, Daniel F. Jaramillo, Michael Proctor, Jochen Kumm, Ronald W. Davis, Corey Nislow, Guri Giaever
Biochemistry, Stanford Genome Technology Cen, 855 California Ave., Palo Alto, CA, 94304, US
450CGenomicsMicroarray analysis of early aging in yeast.
Jennifer Hardee (1), Rishi Jindal (1), Michelle Wu (1), Michelle Yuen Shimogawa (1), Allen Kuo (1), Laty Cahoon (1), Johanna Hardin (2), Laura L. Mays Hoopes (1)
(1) Biology and Molecular Biology, Pomona College, 609 N College Ave, Claremont, CA, 91711, USA; (2) Department of Mathematics, Pomona College, 610 N College Ave, Claremont, CA 91711 USA
451AGenomicsGenome-wide analysis of heterologous protein production in yeast.
Hisashi Hoshida, Rinji Akada
Appl. Chem. and Chem. Eng., Yamaguchi University, 2-16-1 Tokiwadai, Ube, 755-8611, Japan
452BGenomicsTranscript profiling of Saccharomyces cerevisiae during acetic acid stress, apoptosis and necrosis.
Björn Johansson (1), Mark Ramsdale (2), Manuela Corte-Real (1)
(1) Departamento de Biologia, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal; (2) Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK
453CGenomicsGenome-wide analysis of Saccharomyces cerevisiae mutant strains expressing a recombinant human protein.
Eun Jung Han (1), Eun Jeong Kang (1), Woo Kyu Kang (1), Hyun Ah Kang (2), Jeong-Yoon Kim (1)
(1) Dept. of Microbiology, Chungnam National University, Gung-dong, Yuseong, Daejeon, 305-764, Korea; (2) Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea
454AGenomicsSmall is beautiful and meaningful: Identification and characterization of small ORFs (sORFs) in S. cerevisiae.
James Kastenmayer, Wei-Chun Au, Carole Carter, Munira Basrai
Genetics, CRC, National Cancer Institute/NIH, 8901 Wisconsin Ave., Bethesda, MD, 20889-5101, USA
455BGenomicsGlobal analysis of aging in yeast.
Matt Kaeberlein (1), Kathryn T. Kirkland (2), Joe Rogers (2), Stan Fields (1), Brian K. Kennedy (2)
(1) Departments of Genome Sciences and Medicine, University of Washington, Box 357730, Seattle, WA 98195; (2) Department of Biochemistry, University of Washington, Box 357350, Seattle, WA, 98195, USA
456CGenomicsGenome wide functional analysis of siderophore iron uptake in Saccharomyces cerevisiae.
Simon A. B. Knight (1), Renata Santos (2), Jean-Michel Camadro (2), Andrew Dancis (1), Emmanuel Lesuisse (2)
(1) Medicine, Div. Hem/Onc, University of Pennsylvania, 421 Curie Blvd, Philadelphia, PA, 19104, USA; (2) Institut Jacques Monod, Université Paris 7, 2 place Jussieu, 75251, Paris cedex 05, France
457AGenomicsLarge-scale mutagenesis of the yeast genome using a Tn7-derived multipurpose transposon.
Michael Seringhaus (1), Matthew Biery (2), Robert Sarnovsky (2), Lara Umansky (1), Stacy Piccirillo (1), Sandra Matson (1), Matthew Heidtman (1), Kei-Hoi Cheung (3), Craig Dobry (4), Mark Gerstein (1), Nancy Craig (2), Michael Snyder (1), Anuj Kumar (4)
(1) Dept. of Mol. Biophysics and Biochem., Yale University, P.O. Box 208114, New Haven, CT 06520-8114; (2) Dept. of Mol. Biology and Genetics, Johns Hopkins Univ. School of Med., 725 N. Wolfe Street, Baltimore, MD 21205; (3) Center for Medical Informatics, Yale University School of Medicine, New Haven, CT 06510; (4) MCDB and Life Sciences Inst., University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI, 48109-2216, USA
458BGenomicsGenomic response of Saccharomyces cerevisiae to short-term anaerobiosis: differential response and role of Msn2/4 in galactose and glucose media.
Liang-Chuan Lai (1), Alexander L. Kosorukoff (2), Patricia V. Burke (1), Kurt E. Kwast (1)
(1) Mol. & Int. Physiology, University of Illinois, 407 S. Goodwin Ave, Urbana, IL, 61801, USA; (2) Department of Computer Science, University of Illinois, 201 N. Goodwin Ave., Urbana, IL, 61801
459CGenomicsSynthetic genetic and phenotypic analysis of gene modules involved in chitin synthesis.
Guillaume Lesage (1), Jesse Shapiro (1), Charles Specht (2), Arnaud Firon (1), Anne-Marie Sdicu (1), Patrice Menard (1), Shamiza Hussein (1), Amy H.Y. Tong (3), Charles Boone (3), Howard Bussey (1)
(1) Department of Biology, McGill University, 1205 Dr. Penfield, Montreal, QC, H3A 1B1, Canada; (2) Department of Medicine, Boston University, Boston (MA); (3) Banting and Best Department of Medical Research, University of Toronto, Toronto (ON)
460AGenomicsGenomic analysis of stationary phase and exit in Saccharomyces cerevisiae: transcriptional profiling and identification of novel essential genes.
Juanita Martinez (1), Amanda Archuletta (1), Angelina Rodriguez (1), Anthony Aragon (1), Sushmita Roy (2), Chris Allen (1), Peter Wentzell (3), Margaret Werner-Washburne (1)
(1) Department of Biology, University of New Mexico, Redondo Drive, Albuquerque, NM, 87131, USA; (2) Department of Computer Science, University of New Mexico; (3) Department of Chemistry; Dalhousie University; Halifax, Nova Scotia, Canada
461BGenomicsA genomic approach to deciphering G1 transcription regulatory networks in Saccharomyces cerevisiae.
Jonathan Millman, Brenda Andrews
Medical Genetics &Microbiology, University of Toronto, 1 King's College Cr., Toronto, ON, M5S 1A8, Canada
462CGenomicsAdy2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae.
Sandra Paiva (1), Frederic Devaux (2), Sonia Barbosa (1), Claude Jacq (2), Margarida Casal (1)
(1) Departamento de Biologia, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal; (2) Laboratoire de Genetique Moleculaire, CNRS UMR8541, Ecole Normale Superieure, 46 rue d'Ulm 75230 Paris Cedex 05, France
463AGenomicsGenome-wide approach for identification of host genes involved in replication of tombusvirus replicon in yeast.
Tadas Panavas, Elena Serviene, Jeremy Brasher, Peter D. Nagy
Plant Pathology, University of Kentucky, 1405 Veterans Dr., Lexington, KY, 40503, USA
464BGenomicsApplying yeast genomic strategies to link biologically active compounds to their cellular targets.
Ainslie B. Parsons (1), Andres Lopez (1), Renee Brost (1), Tim Hughes (1), Raymond Andersen (2), Charlie Boone (1)
(1) BBDMR, University of Toronto, 112 College Street, Toronto, ON, M5G 1L6, Canada; (2) Department of Chemistry, University of British Columbia
465CGenomicsA genome-wide screen in Saccharomyces cerevisiae reveals altered transport as a mechanism of resistance to the anticancer drug bleomycin.
Mustapha Aouida (1), Nicolas Pagé (2), Anick Leduc (1), Matthias Peter (2), Dindial Ramotar (1)
(1) University of Montreal, Guy-Bernier Research Centre, 5415 de l'Assomption, Montreal, Quebec, Canada, H1T 2M4. Tel: (514) 252-3400 ext 4684; Fax: (514) 252-3430; (2) Swiss Federal Institute of Technology Zurich, Institute of Biochemistry, ETH Hoenggerberg HPM G 10, CH-8093 Zurich (Switzerland), Tel: +41 1/633-4587 - Fax: +41 1/632 1298
466AGenomicsSurface-associated gene expression in yeast biofilms.
Todd Reynolds (1), Kexin Yu (2), Gerald R. Fink (2)
(1) Department of Microbiology, University of Tennessee, F321 Walters Life Sc, Knoxville, TN, 37996, United States; (2) Whitehead Institute for Biomedical Research, Cambridge, MA, 02144
467BGenomicsExperimental validation of a comparative genomics approach for finding transcription factor binding sites.
Linda Riles, Jay Gertz, Su-Wen Ho, Barak A. Cohen
Genetics, Washington University, 4444 Forest Park, Saint Louis, MO, 63108, USA
468CGenomicsGenetic Dissection of Quantitative Trait Loci.
Gal Hagit Romano, Shira Goldshtein, Martin Kupiec
Molec. Microbiology &biotech, Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel
469AGenomicsA Yeast Overexpression Array: Synthetic Dosage Lethality and the Identification of New Pho85 Substrates.
Richelle Sopko (1), Nicolle Preston (2), Dongqing Huang (1), Gordon Chua (2), Kristine Willis (1), Mike Snyder (3), Charlie Boone (2), Brenda Andrews (1)
(1) Medical Genetics, University of Toronto, 1 Kings College Circ, Toronto, ON, M5S 1A8, Canada; (2) Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada; (3) Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut
470BGenomicsTranscriptome profiling of a Candida albicans mutant with a constitutively activated cAMP-mediated pathway.
WonHee Jung (1), Steffen Rupp (2), Nicole Hauser (2), Lubomira Stateva (1)
(1) Dept of Biomolecular Sciences, UMIST, PO Box 88, Manchester, M60 1QD, UK; (2) Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, 12, Nobelstr 70569 Stuttgart, GERMANY
471CGenomicsGenomic analysis reveals mechanisms underlying genetic instability of polyploid cells.
Zuzana Storchova, Jessica Cande, Amanda Breneman, Joshua Dunn, David Pellman
Pediatric Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA, 02116, USA
472AGenomicsSystematic construction of the viable deletion mutants in the Sigma1278b background using the synthetic genetic array (SGA) technology.
Amy HY Tong (1), Todd Reynolds (2), Gerald Fink (3), Charles Boone (1)
(1) Banting & Best Dept of Med Res, Best Institute, 112 College St, Toronto, ON, M5G 1L6, Canada; (2) Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA.; (3) 4Whitehead Institute, MIT, 9 Cambridge center, Cambridge, MA 02142, USA.
473BGenomicsCombining functional genomics and drug screening to identify new components of the rapamycin signaling pathway.
Kristine Willis, Richelle Sopko, Brenda Andrews
Department of Medical Genetics, University of Toronto, 1 King's College Cir, Toronto, ON, M5S1A8, Canada
474CGenomicsYeast genetic interaction network is a scale-free network.
Xiaofeng Xin (1), Gary Bader (2), Amy Tong (1), Charlie Boone (1)
(1) Dept. of Mol. & Med. Genetics, University of Toronto, 1 King's College Cir, Toronto, ON, M5S 1A8, Canada; (2) Computational Biology Center, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, Box 460, 10021, New York, NY, USA
475AGenomicsIdentification and characterization of bottom fermenting yeast-specific genes.
Satoshi Yoshida (1), Kaori Hashimoto (1), Emiko Shimada (1), Tatsuji Ishiguro (1), Satoru Mizutani (1), Kosuke Tashiro (2), Satoru Kuhara (2), Toshiko Minato (1), Hiroyuki Yoshimoto (1), Osamu Kobayashi (1)
(1) Cent. Labs. for Key Technology, Kirin Brewery Co., Ltd., 1-13-5 Fukuura, Yokohama, 236-0004, Japan; (2) Graduate School of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 Japan
476BGenomicsGenome-wide expression analysis of sugar-induced cell death in the bottom fermenting yeast Saccharomyces pastorianus.
Hiroyuki Yoshimoto (1), Rie Ohuchi (1), Kumiko Ikado (1), Satoshi Yoshida (1), Toshiko Minato (1), Tatsuji Ishiguro (1), Satoru Mizutani (1), Kosuke Tashiro (2), Satoru Kuhara (2), Osamu Kobayashi (1)
(1) Central Labs. for Key Tech., Kirin Brewery, Co., Ltd., Fukuura, Kanazawa-ku, Yokohama-shi, 236-0004, Japan; (2) Graduate School of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
477CGenomicsThe transcriptional response of Saccharomyces cerevisiae to the plasma membrane-perturbing compound chitosan.
Anna Zakrzewska, Andre Boorsma, Klaas J. Hellingwerf, Stanley Brul, Frans M. Klis
SILS-Microbiology, University of Amsterdam, Nwe.Achtergracht 166, Amsterdam, 1018WV, The Netherlands
478AProteomicsFunctional organization of the yeast protein complexes responding to cadmium ions.
Weon Bae, Xian Chen
Bioscience Division (B2), Los Alamos National Laboratory, MS M888, Los Alamos, NM, 87545, USA
479BProteomicsThe GPCR-interactome: a comprehensive membrane protein interaction map of the human G-Protein Coupled Receptors (GPCRs).
Lukas Bürkle (1), Michael Fetchko (1), Daniel Auerbach (2), Igor Stagljar (1)
(1) Vet. Biochem and Mol Biology, Universtiy of Zürich-Irchel, Winterthurerstr. 190, Zurich, CH-8057, Switzerland; (2) Dualsystems Biotech Inc., Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
480CProteomicsProteomics of totally unstructured proteins using Saccharomyces cerevisiae as a prototype organism.
Marc S. Cortese (1), Yugong Cheng (2), Vladimir N. Uversky (3), A. Keith Dunker (4)
(1) Indiana University School of Medicine, Department of Biochemistry and Molecular Biology, 635 Barnhill Dr., MS4023, Indianapolis, IN 46202; (2) Molecular Kinetics, Inc., Indiana University Emerging Technology Center, 351 West 10th Street, Indianapolis, IN 45202; (3) Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064; (4) Center for Computational Biology and Bioinformatics, University of Indiana School of Medicine, Indianapolis, IN 46202
481AProteomicsInvestigating the role of phosphorylation in transcription initiation using protein chips.
Geeta Devgan (1), Jason Ptacek (1), Heng Zhu (1), Greg Michaud (2), Xiaowei Zhu (1), Barry Schweitzer (2), Paul Predki (2), Michael Snyder (1)
(1) Department of MCDB, Yale University, 266 Whitney Avenue, new haven, CT, 06520, USA; (2) Protometrix, Inc., 688 East Main Street, Branford, CT 06405
482BProteomicsA novel proteomics approach to analyze interactions among yeast DNA processing proteins.
Michael Fetchko, Ingrid Stoffel-Studer, Jacqueline Hort, Igor Stagljar
Vet. Biochem and Mol Biology, Universtiy of Zürich-Irchel, Winterthurerstr. 190, Zurich, CH-8057, Switzerland
483CProteomicsA collection of S. cerevisiae C-terminal ORF fusions in a versatile vector.
Daniel Gelperin (1), Martha Wilkinson (2), Michael White (2), Nelson Lopez-Hoyo (1), Yoshiko Kon (2), Lixia Jiang (1), Stacy Piccirillo (1), Haiyuan Yu (3), Mark Gerstein (3), Mark Dumont (2), Eric Phizicky (2), Michael Snyder (1), Elizabeth Grayhack (2)
(1) MCDB, Yale University, 266 Whitney Avenue, New Haven, CT, 06520, USA; (2) Department of Biochemistry and Biophysics, University of Rochester School of Medicine Rochester NY 14642; (3) Department of Molecular Biophysics and Biochemistry, Yale University, New Haven CT 06520-8103
484AProteomicsMerging proteomics and genomics: High throughput genome-wide analysis of DNA sequences that bind transcription factors in S. cerevisiae..
Su-Wen Ho (1), Ghil Jona (2), Linda Riles (1), Michael Snyder (2), Mark Johnston (1)
(1) Genetics, Washington University, 4444 Forest Park, Saint Louis, MO, 63108, USA; (2) Dept. of Mol. Cell. & Dev. Biology, Yale University, P.O.Box 208103, New Haven CT 06520-8103 , USA
485BProteomicsA systematic approach to study membrane protein interactions in yeast.
Kavitha Iyer (1), Kim Engels (1), Safia Thaminy (1), Amy Tong (2), Charlie Boone (2), Max Kotlyar (3), Igor Jurisica (3), Daniel Auerbach (4), Igor Stagljar (1)
(1) Vet. Biochem. and Mol Biology, University of zürich-Irchel, Winterthurerstr. 190, Zürich, CH-8057, Switzerland; (2) Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, M5G 1L6 Canada; (3) Ontario Cancer Institute, Princess Margaret Hospital, Toronto, ON M5G 2M9 Canada; (4) Dualsystems Biotech Inc, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
486CProteomicsAn interaction network of yeast integral membrane proteins.
John P. Miller (1), Russell Lo (2), Cynthia Desmarais (1), Igor Stagljar (3), William S. Noble (1), Stanley Fields (2)
(1) Genome Sciences, University of Washington, 1959 NE Pacific St, Seattle, WA, 98125, United States of America; (2) Howard Hughes Medical Institute, Departments of Genome Sciences and Medicine, University of Washington, Box 357730, Seattle, WA 98195-7730; (3) Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
487AProteomicsCluster analysis of proteomic data reveals a novel component of SAGA.
David W. Powell, Connie M. Weaver, Jennifer L. Jennings, K. Jill McAfee, Andrew J. Link
Microbiology and Immunology, Vanderbilt Medical Center, 1161 21st Avenue S., Nashville, TN, 37232-2362, United States of America
488BProteomicsMixed isotope distribution pattern decomposition for quantitative proteomics.
Steven Seeholzer, Thomas Davis, Anthony Yeung, Adrian Canutescu, Lisa Rutkowski, Randy Strich
Cell and Developmental Biology, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA, 19111, USA
489CProteomicsProtein-protein interactions of the PX domain family in yeast.
Carolina Vollert, Peter Uetz
Institute of Genetics, Forschungszentrum Karlsruhe, POB 3640, Karlsruhe, 76021, Germany
490AProteomicsProteome progression upon amino acid starvation in S. cerevisiae.
Oliver Valerius (1), Verena Pretz (2), Juergen Thiermann (2), Gerhard H. Braus (2)
(1) Dept. of Mol. Microbiol. & Genetics, Grisebachstr. 8, 37077 Goettingen, Germany; (2) Mol. Microbiol. & Genetics, Georg-August University, Grisebachstr. 8, Goettingen, G, 37077, Germany
491BProteomicsA new C-terminal ORF-fusion collection for biochemical analysis of the proteome.
Martha Wilkinson (1), Michael White (1), Daniel Gelperin (2), Yoshiko Kon (1), Lixia Jiang (2), Stacy Piccirillo (2), Haiyuan Yu (3), Nelson Lopez-Hoyo (2), Mark Gerstein (3), Mark Dumont (1), Eric Phizicky (1), Elizabeth Grayhack (1), Michael Snyder (2)
(1) Biochemistry and Biophysics, University of Rochester, 601 Elmwood Ave, Rochester, NY, 14642, USA; (2) Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven CT 06520-8103; (3) Department of Biochemistry and Biophysics, Yale University, New Haven CT 06520-8103
492CTechnologyPoster Award: Honorable Mention  Development of a method to reduce recessive background effects of the gene disruption library.
David Alvaro (1), Michael Lisby (2), Rodney Rothstein (2)
(1) 701 W 168th St, HHSC 1606, New York, NY 10032; (2) Genetics and Development, Columbia University, 701 W. 168th St., New York, NY, 10032, USA
493ATechnologyA novel genetic screening method based on artificially forced protein interactions.
Michael DeVit (1), Meg Branson (1), Paul Cullen (2), George Sprague, Jr (2), Stan Fields (1)
(1) Dept of Genome Sciences, University of Washington, 1959 Pacifc Ave NE, Seattle, WA, 98195, USA; (2) Institute of Molecular Biology, University of Oregon, Eugene, OR 97403
494BTechnology4D-imaging of living yeast cells: analysing protein and organelle dynamics in vivo.
Heimo Wolinski, Klaus Natter, Sepp D. Kohlwein
Molecular Biosciences, University Graz, Schubertstr.1, Graz, A8010, Austria
495CTechnologyA tethered catalysis two-hybrid system to identify protein-protein interactions requiring post-translational modifications.
Min-Hao Kuo (1), Tony Hazbun (2), Dawei Guo (1), Xin-Jing Xu (1), Sze-Ling Ng (1), Stanley Fields (2)
(1) Biochem. Mol. Biol., Michigan State University, 309 BCH Building, De, East Lansing, MI, 48824, United States; (2) Howard Hughes Medical Institute, Departments of Genome Sciences and Medicine, University of Washington, Box 357730, Seattle, WA 98195-7730
496ATechnologySelection and monitoring of wild yeast as starter cultures and study of yeast evolution in industrial fermentations.
M. E. Rodríguez (1), J. J. Infante (2), M. L. Espinazo (1), L. Rebordinos (1), J. M. Cantoral (1)
(1) Microbiology, University of Cadiz, Polígono S. Pedro, Puerto Real, 11510, Spain; (2) University of Washington , Department of Biochemistry BOX 357350 , 1959 NE Pacific St. , Seattle WA, 98195-7350
497BTechnologyImproved microarray methods for profiling the Yeast Deletion strain collection.
Daniel Yuan (1), Xuewen Pan (1), Rafael Irizarry (2), Jef Boeke (1)
(1) Molecular Biology and Genetics, Johns Hopkins Univ Sch Med, 725 N Wolfe St, Baltimore, MD, 21205, USA; (2) Biostatistics, Johns Hopkins Univ School of Public Health, 615 N Wolfe St, Baltimore MD 21205
498CInformatics/Computational biologyReconstruction and validation of Saccharomyces cerevisiae iND750, a fully compartmentalized genome-scale metabolic model.
Natalie C. Duarte (1), Markus J. Herrgard (2), Bernhard O. Palsson (2)
(1) 9500 Gilman Dr, La Jolla, CA 92093-0412; (2) Bioengineering, Univ of California, San Diego, 9500 Gilman Dr, La Jolla, CA, 92037-0412, USA
499AInformatics/Computational biologyBioinformatical recognition of Potassium-channel sequences.
Burkhard Heil (1), Jost Ludwig (1), Thomas Lengauer (2), Hella Lichtenberg-Fraté (1)
(1) IZMB, Molekulare Bioenergetik, Universität Bonn, Kirschallee 1, Bonn, 53115, Germany; (2) Max Planck Institut für Informatik Stuhlsatzenhausweg 85 , 66123 Saarbrücken, Germany
500BInformatics/Computational BiologyCarbon Source and Oxygen Regulation in Saccharomyces cerevisiae - Insights from a Transcriptionally Regulated Metabolic Model.
Markus J. Herrgard, Baek-Seok Lee, Bernhard O. Palsson
Department of Bioengineering, UC San Diego, 9500 Gilman Dr, La Jolla, CA, 92093-0412, USA
501CInformatics/Computational biologyGenomic identification and characterization of multidrug resistance genes in yeast.
Maureen E. Hillenmeyer, William Lee, Ronald W. Davis, Corey Nislow, Guri Giaever
Biomedical Informatics, Stanford University, 251 Campus Drive, Stanford, CA, 94305, United States
502AInformatics/Computational biologyGBrowse at the Saccharomyces Genome Database: a customized view of the S. cerevisiae genome.
Eurie L. Hong (1), Rama Balakrishnan (1), Karen R. Christie (1), Maria C. Costanzo (1), Kara Dolinski (2), Selina S. Dwight (1), Stacia R. Engel (1), Dianna G. Fisk (1), Jodi E. Hirschman (1), Robert Nash (1), Anand Sethuraman (1), Chandra L. Theesfeld (1), Gail Binkley (1), Qing Dong (1), David Botstein (2), J. Michael Cherry (1)
(1) Dept. of Genetics, Stanford University, 300 Pasteur Drive, Stanford, CA, 94305-5120, USA; (2) Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
503BInformatics/Computational biologyAnalysis of transcription profiles based on transcriptional modules.
Andre Boorsma, Ania Zakrzewska, Klaas J. Hellingwerf, Frans M. Klis
Microbiology, Swammerdam Inst Life Sci, Nieuwe Achtergracht, Amsterdam, 1018 WV, The Netherlands
504CInformatics/Computational biologyA method for recovering network structure from genomic expression profiles using Kullback-Leibler distance and cluster consensus share.
Alexander L. Kosorukoff (1), Liang-Chuan Lai (2), Patricia V. Burke (2), Kurt E. Kwast (2)
(1) Computer Science, University of Illinois, 201 N. Goodwin Ave., Urbana, IL, 61801, USA; (2) Mol. & Int. Physiology, University of Illinois, 407 S. Goodwin Ave, Urbana, IL 61801, USA
505AInformatics/Computational BiologyIn silico screening of budding yeast mutants based on morphological features.
Fumi Kumagai-Sano (1), Masashi Yukawa (1), Atsushi Ikenishi (1), Ayaka Saka (1), Daisuke Watanabe (1), Kintake Sonoike (1), Hiroshi Sawai (2), Miwaka Ohtani (3), Yoichiro Nakatani (4), Jun Sese (4), Akihiro Nakaya (4), Shinichi Morishita (4), Yoshikazu Ohya (1)
(1) Integrated Biosciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, 277-8562, Japan; (2) Institute for Bioinformatics Research and Development, Japan Science and Technology Agency; (3) Department of Computer Sciences, University of Tokyo; (4) Department of Computational Biology, University of Tokyo
506BInformatics/Computational biologyAn extensible open source software system for recording and analyzing data from a microarray experiment.
Thomas Milac, Paulben McElwain
Applied Mathematics, University of Washington, 408 Guggenheim Hall, Seattle, WA, 98195, USA
507CInformatics/Computational biologySequence comparison between the transcription factor binding sites and extracted promoter consensus sequences.
Neng-Wen Lo (1), Yi-Feng Chang (2), S. Wesley Changchien (2)
(1) Animal Science & Biotechnology, Tunghai University, Sec 3, Chungkang Rd, Taichung, 40407, Taiwan; (2) Department of Information Management, Chaoyang University of Technology, Taichung, Taiwan
508AInformatics/Computational BiologyPoster Award: Honorable Mention  Novel method for systematic analysis of essential yeast genes.
Masashi Yukawa (1), Daisuke Fukuoka (1), Kintake Sonoike (1), Hiroshi Sawai (2), Shinichi Morishita (3), Yoshikazu Ohya (1)
(1) Integrated Biosciences, University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, 277-8562, JAPAN; (2) Institute for Bioinformatics Research and Development, Japan Science and Technology Agency; (3) Department of Computational Biology, University of Tokyo
509BEvolution/Comparative genomicsSaccharomyces Genome Database: a resource for fungal comparative genomics.
Maria C. Costanzo (1), Rama Balakrishnan (1), Karen R. Christie (1), Kara Dolinski (2), Selina S. Dwight (1), Stacia R. Engel (1), Dianna G. Fisk (1), Jodi E. Hirschman (1), Eurie L. Hong (1), Robert Nash (1), Anand Sethuraman (1), Barry Starr (1), Chandra L. Theesfeld (1), Rey Andrada (1), Gail Binkley (1), Qing Dong (1), David Botstein (2), and J. Michael Cherry (1)
(1) Department of Genetics, Stanford U. School of Medicine, 300 Pasteur Drive, Stanford, CA, 94305-5120, USA; (2) Lewis-Sigler Institute for Integrative Genomics, Carl Icahn Laboratory, Princeton University, Washington Rd., Princeton, NJ 08544, USA
510CEvolution/Comparative genomicsGenomic analysis of experimental evolution in S. cerevisiae.
Maitreya Dunham (1)
(1) Lewis-Sigler Institute, Princeton University
511AEvolution/Comparative genomicsInvestigation of genomic differences among wine yeasts by array karyotyping.
Barbara Dunn (1), R. P. Levine (2), Gavin Sherlock (1)
(1) Dept. of Genetics, Stanford University Med. Sch., 300 Pasteur Ave, Stanford, CA, 94305-5120, USA; (2) Dept. of Biological Sciences, Stanford University, Stanford, CA 94305
512BEvolution/Comparative genomicsPhylogenetic footprinting indicates a role for Rap1p in the differential expression of neighboring genes.
Justin Gerke, Barak Cohen
Genetics, Washington University, 4444 Forest Park Bvd, St. Louis, MO, 63108, United States
513CEvolution/Comparative genomicsEvolutionary chomping of the mating-type chromosome in yeasts with the HO gene.
Jonathan L. Gordon (1), Kenneth H. Wolfe (2)
(1) Genetics Department, Trinity College Dublin, Dublin2, Ireland.; (2) Genetics, Trinity College Dublin, College Green, Dublin, D2, Ireland
514AEvolution/Comparative genomicsContribution of horizontal transfer to the evolution of Saccharomyces cerevisiae and Ashbya gossypii.
Charles Hall, Jackie Ou, Fred Dietrich
MGM, Duke University Mecial Center, 286 Carl Research Dr, Durham, NC, 27710, USA
515BEvolution/Comparative genomicsComparative proteomics: differential protein expression in a winemaking strain of Saccharomyces cerevisiae.
Anthony Heinrich (1), Jelle Lahnstein (2), Van Dyk Derek (3), Connolly Angela (3), Jiranek Vladimir (2), de Barros Lopes Miguel (1)
(1) The Australian Wine Research Institute, PO Box 197, Glen Osmond, SA, 5064, Australia; (2) School of Agriculture and Wine, The University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia; (3) Australian Proteome Analysis Facility, Macquarie University, North Ryde, NSW, 2109, Australia
516C
Poster Withdrawn/Cancelled
517AEvolution/Comparative genomicsPoster Award: 1st Place  The impact of polyploidy on yeast transcription factor evolution.
Devin Scannell (1), Mario Fares (2), Ken Wolfe (1)
(1) Genetics Department, Trinity College Dublin, College Green, Dublin, D2, Ireland; (2) Biology Department, N.U.I. Maynooth, Maynooth , Co. Kildare, Ireland
518BEvolution/Comparative genomicsGene and segmental duplications in Saccharomyces cerevisae.
Joseph Schacherer, Yves Tourrette, Jean-Luc Souciet, Serge Potier, Jacky de Montigny
Lab. de Microbio. et Génétique, Université Louis Pasteur, 28, rue Goethe, Strasbourg, 67083, France
519CEvolution/Comparative genomicsUnderstanding the meaning of protein sequence evolution.
Barry Williams, Jayne Selegue, Sean Carroll
Lab of Molecular Biology, University of Wisconsin, 1525 Linden Dr., Madison, WI, 53706, USA
520AEvolution/Comparative genomicsTraces of ancient duplicated copies of the yeast DUP240 multigene family.
Bénédicte Wirth, Véronique Leh-Louis, Serge Potier, Jean-Luc Souciet, Laurence Despons
FRE 2326 ULP/CNRS, Institut de Botanique, 28 rue Goethe, Strasbourg, F-67083, FRANCE
521BEvolution/Comparative genomicsAdaptive evolution of the gene regulatory network in yeast.
Joshua T Witten, Barak A Cohen
Genetics, Washington University, 4444 Forest Park Pky, St. Louis, MO, 63108, USA
522CHuman diseases/Drug discoveryGenome-wide functional analysis of Helicobacter pylori virulence factor CagA in yeast.
Junko Akada (1), Yuji Torigoe (2), Yoshito Kakihara (2), Hisashi Hoshida (2), Hiroaki Aoki (3), Kazuyuki Nakamura (1), Rinji Akada (2)
(1) Dept Biochem & Molec Sencing, Yamaguchi Univ Sch Med, Minami-kogushi, Ube, 755-8505, Japan; (2) Dept Appl Chem & Chem Eng, Yamaguchi Univ, Fac Eng, Ube 755-8611, Japan; (3) Dept Molec Cardiovascular Biol, Yamaguchi Univ Sch Med, Ube 755-8505, Japan
523AHuman diseases/Drug discoveryIsolating small molecule inhibitors of Pseudomonas aeruginosa essential and virulence genes using a yeast phenotypic screen.
Anthony Arnoldo (1), Stephen Lory (2), Leonardo Brizuela (3), Antonio Bedalov (4), Igor Stagljar (1)
(1) Vet. Biochem. and Mol. Biology, University of Zürich-Irchel, Winterthurerstr. 190, Zürich, CH-8057, Switzerland; (2) Institute Microbiology and Mol. Biology, Harvard Medical School, 200 Longwood Av., Waren Alpert 363, Boston, MA 02115, USA; (3) Harvard Institute of Proteomics, 320 Charles St., Cambridge, MA 02141, USA; (4) Clinical Research and Human Biology Divisions, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
524BHuman diseases/Drug discoveryAn inducible defense mechanism against nitric oxide in Candida albicans.
Breanna D. Ullmann (1), Hadley Myers (1), Wiriya Chiranand (1), Anna L. Lazzell (2), Qiang Zhao (1), Luis A. Vega (1), Jose L. Lopez-Ribot (2), Paul R. Gardner (3), Michael C. Gustin (1)
(1) Biochemistry & Cell Biology, Rice University, 6100 Main St, Houston, TX, 77005, United States; (2) Dept. of Medicine, Div. of Infectious Diseases, The University of Texas Health Science Center at San Antonio., San Antonio, Texas 78245; (3) Division of Critical Care Medicine, Children's Hospital Medical Center, Cincinnati, Ohio 45229
525CHuman diseases/Drug discoveryIdentification of novel nuclear receptor coactivators using a functional yeast based screen.
Katie Freeman (1), Hinayana Bawagan (1), Lakshman Ramamurthy (2)
(1) Comparative Genomics, GlaxoSmithKline, 1250 S. Collegeville, Collegeville, PA, 19426, USA; (2) Bioinformatics, GlaxoSmithKline, RTP, NC
526AHuman diseases/Drug discoveryExtensive MHC class I-restricted CD8 T lymphocyte responses against various yeast genera in humans.
Tanja Heintel (1), Frank Breinig (2), Manfred J. Schmitt (2), Andreas Meyerhans (1)
(1) Department of Virology, Saarland University, Building 47, Homburg/Saar, 66421, Germany; (2) Department of Applied Molecular Biology, Saarland University, 66123 Saarbrücken, Germany
527BHuman diseases/Drug discoveryGenome-wide functional analysis of Campylobacter cytolethal distending toxin in yeast.
Takao Kitagawa, Hisashi Hoshida, Rinji Akada
Dept Appl Chem & Chem Eng, Yamaguchi Univ, Fac Eng, Tokiwadai 2-16-1, Ube, 755-8611, Japan
528CHuman diseases/Drug discoverySurrogate genetic analysis of human cyclin A1 oncogenic activity.
Ed Perkins, Kim Schoonover, Donna Parke
Dept. of Biochemistry, Univ. of Minnesota Med. School, 10 University Drive, Duluth, MN, 55812, United States
529AHuman diseases/Drug discoveryUsing yeast to analyze the human promyelocytic leukemia protein (PML).
Boots Quimby, Vladimir Yong-Gonzalez, Alex Strunnikov, Mary Dasso
LGRD, NIH/NICHD, 9000 Rockville Pike, Bethesda, MD, 20816, USA
530BHuman diseases/Drug discoveryTAC1 (Transcriptional Activator of CDR genes) is a new transcription factor involved in the regulation of the Candida albicans multidrug ABC-transporters CDR1 and CDR2.
Alix Coste, Mahir Karababa, Françoise Ischer, Jacques Bille, Dominique Sanglard
Institute of Microbiology, University Hospital Lausanne, Rue du Bugnon 48, Lausanne, 1011, Switzerland
531CHuman diseases/Drug discoveryA chemogenomic approach to understanding the function of the essential phosphatase Glc7.
Robert P. St.Onge, Daniel F. Jaramillo, Ronald W. Davis, Corey Nislow, Guri Giaever
Biochemistry, Stanford University, 855 California Ave, Palo Alto, CA, 94304, United States
532AOther yeastsSNO1 and SNZ1 expression is associated with stationary phase in Candida albicans.
Priya Uppuluri, W. LaJean Chaffin
Microbiology and Immunology, Texas Tech Univ. HSC, 3601 4th St, Lubbock, TX, 79430, USA
533BOther yeastsUsing Bionumerics software and DNA fingerprinting gels to display the diversity in a collection of yeast isolated from fresh and ensiled forages.
Russell K. Chan, Brenda K. Smiley, Cora R. Wortman, Kris P. Heilmann, Kimberly J. Forrester, William M. Rutherford
Forage Additives Research, Pioneer Hi-Bred Int'l, Inc., P. O. Box 1004, Johnston, IA, 50131, U. S. A.
534COther yeastsPartial purification and characterisation of Candida nodaensis Killer toxin.
Sónia da Silva (1), Cristina Aguiar (1), Paula Verissimo (2), Euclides Pires (2), Candida Lucas (1)
(1) Biology, Minho University, Campus Gualtar, Braga, 4710-057, Portugal; (2) CB-Departamento de Biologia, Universidade do Minho, Braga,Portugal.
535AOther yeastsA transformation system for the yeast, C. oleophila, and the role of its exonuclease encoded by CoEXG1, in biocontrol.
Hila Yehuda (1), Samir Droby (2), Michael Wisniewski (3), Martin Goldway (1)
(1) MIGAL, Galilee Technology Center; (2) Agricultural Research Organization, the Volcani Center; (3) Appalachian Fruit Research Station, USDA-ARS
536BOther yeastsYeast orthologues associated with glycerol transport and metabolism.
Luísa Neves, Rui Oliveira, Cândida Lucas
Department of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal
537COther yeastsNitrogen source regulation of polyene susceptibility in Candida albicans.
Brian Oliver, Ted White
Microbial Pathogenesis, SBRI, 307 Westlake Ave N, Seattle, WA, 98109, USA
538AOther yeastsGene Disruption of the Candida albicans ECM22 gene increases susceptibility to a wide range of antifungal drugs.
Peter Silver, Dr. Brian Oliver, Dr. Ted White
Pathobiology, SBRI and Univ. of Washington, 307 westlake ave. N, Seattle, 98109, USA
539BOther yeastsRapid Identification of Yeasts through the MALDI-Mass Spectrometric Analysis of Whole Cells.
Elena Stamenova, Jim Zhou
Mycology, American Type Culture Collecti, 10801 University Blv, Manassas, VA, 20110, USA
540CGlobal Analysis: OtherAmplitude control of cell cycle waves.
Attila Becskei (1), Monica Boselli (2), Alexander van Oudenaarden (1)
(1) Physics Room 13-2009, MIT, 77Massachusetts Ave., Cambridge, MA, MA 02139, USA; (2) Center for Cancer Research, MIT, Cambridge, MA 02139
541AGlobal Analysis: OtherS. cerevisiae annotation in the genomic era.
Dianna Fisk (1), Robert Nash (1), Rama Balakrishnan (1), Karen Christie (1), Maria Costanzo (1), Kara Dolinski (2), Selina Dwight (1), Stacia Engle (1), Jodi Hirshman (1), Eurie Hong (1), Anand Sethuraman (1), Chandra Theesfeld (1), David Botstein (2), J. Michael Cherry (1)
(1) Saccharomyces Genome Database, Stanford University, 300 Pastuer Dr., Stanford, CA, 94305, USA; (2) Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
542BGlobal Analysis: OtherControlled lysis of yeast in the gut for the delivery of vaccines, probiotics and therapeutic agents.
Kalliope Panoutsopoulou (1), Paul Gitsham (2), Laura Ingram-Edwards (1), Yaroslav Terentjev (1), Stephen G. Oliver (2), Geoff Warhurst (3), Lubomira Stateva (1)
(1) Dept. of Biomolecular Sciences, UMIST, PO BOX 88, Manchester, M60 1QD, UK; (2) School of Biological Sciences, Level 1, Wing A , Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK; (3) Gut Barrier Research Group, Clinical Sciences, Hope Hospital, Salford M6 8HD
543CGlobal Analysis: OtherScaling Up SLAM (Synthetic Lethality on Microarrays): Technical and Biological Lessons.
Carol Tiffany (1), Xuewen Pan (2), Daniel Yuan (2), Courtney Hollender (1), Sharon Sookhai-Mahadeo (2), Xiaoling Wang (2), Brian Peyser (1), Ping Ye (3), Joel Bader (3), Jef Boeke (2), Forrest Spencer (1)
(1) Institute of Medical Genetics, Johns Hopkins University, 720 Rutland Ave, Baltimore, MD, 21205, USA; (2) Molecular Biology & Genetics, Johns Hopkins University, Baltimore, MD 21205; (3) Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218
544A
Improved ZIP1::GFP as a probe for analyzing meiosis in Saccharomyces cerevisiae.
H. Wang, E. White and D.B. Kaback
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, NJ 07103, USA
545B
Assessment of uncharacterised proteins implicated in meiotic DNA processing.
Philip W. Jordan and David R. Leach.
Institute of Cell and Molecular Biology, The University of Edinburgh, Mayfield Road, Edinburgh, EH9 3JR, Great Britain
546C
Modelling the mechanism of spore-number control in yeast meiosis.
Christof Taxis, Zaharoula Kavagiou, Mike Stein, Ernst. E. Stelzer, Michael Knop.
Cell Biology and Cell Biophysics Programme, EMBL, Meyerhofstr. 1, D-69117 Heidelberg, Germany
547A
Regulation recruitment is sufficient for complex combinatorial transcription control.
Nicolas E. Buchler (1), Ulrich Gerland (2), Terence Hwa (3)
(1) Center for Studies in Physics and Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA; (2) Ludwig-Maximillians Uniiversity, Munich, Germany; (3) University of California at San Diego, La Jolla, CA, USA.
548B
Intracellular microbe in baker's yeast.
Timothy Deimling, Zhaojie Zhang, and Theodor Hanekamp.
Department of Molecular Biology, University of Wyoming, University Avenue, Laramie, WY 82071-3944, USA
549C
Mre11p regulates the onset of Telomeric recombination.
Immanual Joseph (1), Mark Tidwell (1), Bibo Li (2), and Arthur J. Lustig (1)
(1) Department of Biochemistry, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA, USA; (2) Laboratory of Molecular Parasitology, The Rockefeller University, New York, NY, USA.
550A
Differential regulation of Tec1 by Fus3 and Kss1 confers signaling-specificity in yeast development.
Tim Köhler (1), Stefan Brüuckner (1), Gerhard Braus (2), Barbara Heise (1), Hans-Ulrich Mösch (1)
(1) Department of Genetics of Eukaryotic Microorganisms, Georg-August-University, Grisebachstr. 8, 37077 Göttingen, Germany; (2) Department of Molecular Microbiology and Genetics, Georg-August-University Goöttingen, Germany
551A
Role of the AP-1 adaptor complex in trafficking between the trans-Golgi network and endosomal system.
Christopher Foote and Steven F. Nothwehr
Division of Biological Sciences, 401 Tucker Hall, University of Missouri-Columbia, Columbia, Missouri, 65211, USA.
552C
Identifying Factors involved in Cellular Response to Arsenic.
Michael Thorsen (1), Erik Kristiansson (2), Olle Nermann (2), Markus Tamas (1)
(1) Department of Cell and Molecular Biology / Microbiology, Göteborg University, Sweden; (2) Department of Mathematical Statistics, Chalmers University of Technology, Göteborg, Sweden
553A
Physical and functional interplay of the deubiquitinating enzyme Ubp2, the E3 ligase Rsp5, and a protein of unknown function, Rup1
Mandy H.Y. Lam (1,2), Mike Davey (1), Jack F. Greenblat (1,2), & Andrew Emili (1,2)
(1) Banting and Best Department of Medical Research, University of Toronto, 112 College St., Toronto, ON, M5G 1L6, Canada; (2) Department of Medical Genetics and Microbiology, University of Toronto. Canada
554B
Proteasome involvement in the repair of DNA double-strand breaks.
Mandy H.Y. Lam (1,2,+), Nevan J. Krogan (1,2,+), Jeffrey Filingham (1,+), Marinella Gebbia (1), Joyce Li (1), Nira Datta (1), Xiaorong Wu (1), Gerand Cagney (1), Jack F. Greenblatt (1,2), & Andrew Emili (1,2)
(1) Banting and Best Department of Medical Research, University of Toronto, 112 College St., Toronto, ON, M5G 1L6, Canada; Department of Medical Genetics and Microbiology, University of Toronto, Canda; (+) These authors contributed equally to this work.
555C
Mechanism of illegitimate recombination in Kluyveromyces lactis.
Andreas Kegel (1), Sidney Carter (1), Monique-Bolotin-Fukuhara (2), Stefan Äström
(1) Stockholms University, Arrheniuslab E3, Svante Arrheniusv. 16, 10691 Stockholm, Sweden; (2) Institut de Genetique et Microbiologie, Batiment 400, Universite Paris-Sud, 91405 Orsay Cedex, France
556A
Applications of functional proteome Yeast ProtoArrays™
Gregory A. Michaud, Fang Zhou, Rhonda Bangham, Michael Salcius, Jaclyn Bonin, Barry Schweitzer and Paul F. Predki
Protometrix, Inc., 688 East Main Street, Branford, CT 06405, USA
557B
Yeast proteins involved in rRNA and ribosome biosynthesis are transcriptionally co-regulated.
Christopher Wade, Mark Umbarger and Michael McAlear
Molecular Biology and Biochemistry Department, Wesleyan University, Lawn Avenue Hall-Atwater Labs, Middletown, CT 06459, USA
558C
Generating membrane curvature during yeast prospore membrane biogenesis due to a lipid modification pathway.
Peter M. Maier (1), Alexandra C. Moreno-Borchart (1), Jens Knudsen (2), Nils J. Færgeman (2) and Michael Knop (1)
(1) European Molecular Biology Laboratory, Meyerhofstr. 1, 69117 Heidelberg, Germany; (2) University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark
559A
Proteome Explorer.
Roger Kramer, Cammie Lesser
Department of Medicine (Microbiology and Molecular Biology) MGH/HMS, 65 Landsdowne St., Rm 417 Cambridge, MA 02139, USA
560B
Downregulation of tRNA splicing in cells with DNA damage.
Ata Ghavidel and Andrew Emili
Banting and Best Department of Medical Research, University of Toronto, 112 College St., Toronto, ON, M5G 1L6, Canada
561C
Protein Complexes and Functional Pathways in Chromatin Metabolism.
Nevan J. Krogan (1,2), Nira Datta (1), Jeffrey Pootoolall (1), Jonathan Weissman (3), Erin O'Shea (3), Charles Boone (1,2), Andrew Emili (1,2), Timothy Hughes (1,2), Jack Greenblatt (1,2)
(1) Banting and Best Department of Medical Research, University of Toronto, 112 College St., Toronto, ON, M5G 1L6, Canada; (2) Dept. of Molecular and Medical Genetics, Univ. of Toronto, Toronto, Ontario, M5G 1L6 Canada; (3) Howard Hughes Medical Institute, Univ. of California, San Francisco, CA 94143, USA
562A
Modeling Mechanisms of Shigella Pathogenesis in Yeast.
Naomi Slagowski, Roger Kramer and Cammie Lesser
Department of Medicine (Microbiology and Molecular Biology) MGH/HMS, 65 Landsdowne St., Rm 417 Cambridge, MA 02139, USA
563B
Horizontal transfer of an intein between fungal phyla.
Russell Poulter, Tim Goodwin, Jeremy Gray, Margaret Butler
Department of Biochemistry, University of Otago, 710 Cumberland Street, Dunedin, New Zealand
564C
Differential loss of alternative copies of duplicated genes in paleopolyploid yeast species.
Devin Scannell, Kevin Byrne, Jonathan Gordon, Simon Wong, Ken Wolfe
Smurfit Institute of Genetics, Trinity College Dublin, College Green, Dublin, D2, Ireland
565A
Poster Award: 4th Place  Efficient plasmid transfer into the yeast gene disruption libraries for use with genetic screens.
Robert J.D. Reid (1), Ivana Sunjevaric (1), Warren P. Voth (2), Wendy Du (2), Aileen Olsen (2), David J. Stillman (2), and Rodney Rothstein (1)
(1) Dept of Genetics & Development, Columbia University College of Physicians and Surgeons, New York, NY, USA; (2) Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT, USA
566B
Search for SUP35 mutations that increase the spontaneous appearance of the [PSI+] prion.
Yakov Vitrenko, Susan Liebman
Dept of Genetics & Development, Columbia University College of Physicians and Surgeons, New York, NY, USA

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