Asymmetric
inheritance of oxidatively damaged proteins during cytokinesis.
Hugo Aguilaniu (1),
Lena Gustafsson (1), Michel Rigoulet (2), Thomas Nyström (3)
(1) Molecular Biotechnology, Chalmers University, Box 462, Göteborg, 40530,
Sweden (hugo.aguilaniu@molbiotech.chalmers.se); (2) Institut de Biochimie et de
Génétiques Cellulaires du CNRS, Université Victor Segalen, Bordeaux, France;
(3) Department of Cell and Molecular Biology - Microbiology, Göteborg
University, Göteborg, Sweden
Carbonylated proteins were visualized in single cells of the budding yeast Saccharomyces cerevisiae, revealing that they accumulate with replicative age. Furthermore, carbonylated proteins were not inherited by daughter cells during cytokinesis. Mother cells of a yeast strain lacking the sir2 gene, a life span determinant, failed to retain oxidatively damaged proteins during cytokinesis. These findings suggest that a genetically determined, Sir2p-dependent asymmetric inheritance of oxidatively damaged proteins, may contribute to free radical defense and the fitness of newborn cells.