Assessment of
uncharacterised genes of Saccharomyces cerevisiae implicated in meiotic
DNA processing pathways by integration of high-throughput genetic data.
Philip Jordan
Institute of Cellular and Molecular Biology, University of Edinburgh, Mayfield
Road, EH9 3JR, Edinburgh, Scotland, (p.jordan@ed.ac.uk)
Although there are a large number of Saccharomyces cerevisiae genes involved in meiotic DNA processing, many of the pathways they operate within and functional links between them are not well distinguished. One major reason for the lack of functional knowledge of meiotic DNA processing (MeDNAP) pathways is the fact that approximately one third of the expressed genes of S. cerevisiae are uncharacterised. To help elucidate functions for each gene expressed in S. cerevisiae many high-throughput studies have been performed. The benefits of high-throughput data are countered by the difficulty of assessing the large data sets they produce. Here a four-stage selection process that integrates and correlates data from a number of sources including protein interaction data, expression data, subcellular localisation and bioinformatics has been used to implicate involvement of uncharacterised genes in MeDNAP pathways. Forty uncharacterised genes have been selected in this study, and their implied involvement in a MeDNAP pathway is being experimentally assessed.