Influence of
separate genome element on cell metabolism in yeast Debaryomyces hansenii.
Inna Sydorovych,
Daria Fedorovych
Departm.of Regulatory Systems, Institute of Cell Biology, Dragomanov 14/16,
Lviv, 79005, Ukraine (inn_bohdan@yahoo.com)
Some groups of genes may placed on separate genome elements for best functional role in cells. A collection of mutants of yeast Debaryomyces hansenii VKM Y-102 with eliminated separate linear DNA was received. Metabolism of mutants was characterised by certain properties concerning respiratore metabolism such as elevated levels of iron and riboflavin, alterations in oxidative status of cells, different growth in media with fermentable and nonfermentable carbon sources. Elimination of DNA fragment may be provoked by the action of some transient metals, such as Mn2+ or Cr6+ and may be connected with changes in cell morphology and structure of cell wall. Also mutant cells are flocculent in liquid growth medium and stretch in despite to round wt cells. Its interesting that described phenotype of D. hansenii mutants with simultaneous elevation of riboflavin biosynthesis and iron transport in cells was reminiscenced phenotype of tup1 deletion mutants in yeast Candida albicans [Lessuisse et al., 2002]. But are no evidences for disruption of Tup1 protein or its homolog in D. hansenii mutant cells. We obtained transformants of mutants D. hansenii with DNA fragment from Y-102 cells that its impossible for tup1 deletion cells, while tup1 mutation leads to plasmid unstability. But additional investigation is in order to response this question.