For long researchers have been searching for the triggering signal/s for
glucose repression. There is probably not only one signal and different
genes will be the target for different signals. It has previously been
shown that glucose repression of some genes are correlated to glycolytic
rate. Into a strain where all the glucose transporters have been deleted
(Null strain) chimeras of the low and high affinity transporters HXT1
and HXT7 have been expressed one at a time. These strains have given us
a series of strains with different glucose consumption rates i.e
glycolytic rates (see poster 12-34). In this study we have initially
been looking at three strains: Wild type, HXT7 only expressing strain
and the Null strain. By growing these strains on ethanol and then pulse
them with 5% glucose we have followed the dynamic expression response of
genes exposed to glucose repression and induction using real-time PCR as
a fast, reliable and sensitive method.