XXIth International Conference on Yeast Genetics and Molecular Biology: Invited Lectures

Poster Session 1 - Vesicular transport and membrane biogenesis

  • 1-1
    •   Ent3p an ENTH domain containing protein is required for protein sorting in the multivesicular body.
    Sylvie Friant, Anne Eugster, Eve-Isabelle Pecheur, Francois Letourneur
    UMR5086 du CNRS, IBCP, 7 passage du Vercors, Lyon, 69367, France (s.friant@ibcp.fr)

  • 1-2
    •   Involvement of Cwh8p in the sphigolipids biosynthesis in yeast S. cervisiae.
    Martine Pittet, Andreas Conzelmann
    Institute for Biochemistry, University of Fribourg, Ch. du Musée 5, Fribourg, 1700, Switzerland (martine.pittet2@unifr.ch)

  • 1-3
    •   A self-organization model for the transitional endoplasmic reticulum.
    Daniel E. Strongin (1), Brooke J. Bevis (1), Jon F. Soderholm (1), Matthias Weiss (2), Benjamin S. Glick (1)
    (1) Molec. Genetics and Cell Bio., University of Chicago, 920 E. 58th St., Chicago, IL 60637, USA (destrong@uchicago.edu); (2) Cell Biology and Cell Biophysics Programme, EMBL, Heidelberg, Germany

  • 1-4
    •   The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle tethering factors at the Golgi apparatus.
    Bojana Panic , James R.C. Whyte, Sean Munro
    Cell Biology, MRC-LMB, Hills Road, Cambridge, CB2 2QH, United Kingdom (bpanic@mrc-lmb.cam.ac.uk)

  • 1-5
    •   Tagging the genes that affect peroxisome degradation in the alkane-utilizing yeast Yarrowia lipolytica.
    Taras Y. Nazarko (1), Jean-Marc Nicaud (2), Andrei A. Sibirny (1)
    (1) Molecular Genetics and Biotech, Institute of Cell Biology, Drahomanov St, 14/16, Lviv, 79005, Ukraine (nazarko@biochem.lviv.ua); (2) Laboratoire de Microbiologie et de Genetique Moleculaire, UMR216, URA1925, INRA Centre de Grignon, BP 01, F-78850 Thiverval-Grignon, France

  • 1-6
    •   The identification of Chs5p, Ymr237p and Yhr112p as new interactors of Arf1p.
    Mark Trautwein (1), Robert Gauss (1), Joern Dengjel (2), Markus Schirle (2), Anne Spang (1)
    (1) Friedrich-Miescher-Laboratory, Max-Planck-Society, Spemannstr. 39, Tuebingen, 71076, Germany (mark.trautwein@tuebingen.mpg.de); (2) Department of Immunology, Institute of Cell Biology, University of Tuebingen, Auf der Morgenstelle 15, D-72076 Tuebingen, Germany

  • 1-7
    •   Activity of recycling golgi mannosyltransferases in the yeast endoplasmic reticulum.
    Leena Karhinen, Marja Makarow
    Institute of Biotechnology, University of Helsinki, Viikinkaari 9, Helsinki, 00014, Finland (leena.karhinen@helsinki.fi)

  • 1-8
    •   Regulation of storage lipid synthesis in yeast in aerobic and anaerobic conditions.
    Zuzana Mrózová, Ivan Hapala
    Inst. Anim. Biochem. Genet., Slovak Academy of Sciences, Moyzesova 61, Ivanka pri Dunaji, 90028, Slovak Republic (zuzana.mrozova@savba.sk)

  • 1-9
    •   Different packaging chaperones are required for ER exit of various hexose transporters in Saccharomyces cerevisiae.
    Tanja Hamacher, Andreas Hippe, Eckhard Boles
    Institut fuer Mikrobiologie, J.W.Goethe-Universitaet, Marie-Curie-Str. 9, Frankfurt am Main, D-60439, Germany (Hamacher@em.uni-frankfurt.de)

  • 1-10
    •   Characterisation of Ent3p, Ent4p, and Ent5p, three yeast epsin homologues.
    Anne Eugster, Eve Pecheur, Sylvie Friant, Francois Letourneur
    Universite Lyon 4, IBCP, 7, Passage du Vercor, Lyon, 69763, France (a.eugster@ibcp.fr)

  • 1-11
    •   Triacylglycerol metabolism in Saccharomyces cerevisiae.
    Guenther Daum, Karin Athenstaedt, Daniel Sorger
    Biochemistry, TU Graz, Petersgasse 12/2, Graz, A-8010, Austria (guenther.daum@tugraz.at)

  • 1-12
    •   PEX15p - a player in de novo peroxisome biogenesis?
    Ylva Huber (1), Karin Großschopf (1), Michaela Riepl (1), Andreas Hartig (2)
    (1) Inst.f.Biochemie u. Molekulare, Zellbiologie, Univ. Wien, Dr. Bohrgasse 9, Vienna, A-1030, Austria (yhub@yahoo.com); (2) Ludwig Boltzmann-Forschungsstelle für Biochemie, Vienna Biocenter, Dr. Bohrgasse 9, A-1030 Vienna, Austria

  • 1-13
    •   The role of sphingolipids in transport and stabilization of the yeast plasma membrane ATPase.
    Roger Schneiter (1), Birgit Timischl (1), Linda Giunta (1), Sonja Reiner (2)
    (1) Division of Biochemistry, University of Fribourg, Chemin du Musee 5, Fribourg, 1700, Switzerland (roger.schneiter@unifr.ch); (2) Institute of Biochemistry, Technical University Graz, Austria

  • 1-14
    •   Novel essential genes of Saccharomyces cerevisiae involved in protein secretion.
    Svetlana Davydenko, Sirkka Keränen
    Biotechnology, VTT, Tietotie 2, Espoo, 02044 VTT, Finland (sirkka.keranen@vtt.fi)

  • 1-15
    •   The beta subunit of the Sec61p ER translocon interacts with the exocyst complex in Saccharomyces cerevisiae.
    Jaana Toikkanen, K. Juha Miller, Jussi Jäntti, Sirkka Keränen
    VTT, Biotechnology, Tietotie 2, Espoo, 02044 VTT, Finland (jaana.toikkanen@vtt.fi)

  • 1-16
    •   Characterizing the S. cerevisiae MAPR homologue, YPL170w/DAP1.
    Chris Banna, Phillip Gray, Jung Choi
    Molecular Biology, Georgia Inst. of Technology, 315 Ferst Dr., Atlanta, GA 30332, USA (gt5214b@prism.gatech.edu)

  • 1-17
    •   Identification of new genes involved in intracellular transport.
    Gunilla Barmark, Marie Gustavsson, Jimmy Larsson, Eva Murén, Hans Ronne
    Dep of Plantbiol & Forrestgen, SLU, Box 7080, Uppsala, 750 07, Sweden (Gunilla.Barmark@vbsg.slu.se)

  • 1-18
    •   Specific role in sporulation of the yeast syntaxin Sso1p.
    Mattias Öyen (1), Jussi Jäntti (2), Sirkka Kärenen (2) and Hans Ronne (1).
    (1) Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Box 7080 S-75007 Uppsala, Sweden. (Mattias.Oyen@vbsg.slu.se) (2) VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Finland

  • 1-19
    •   Use1p is a yeast SNARE protein required for retrograde traffic from the Golgi to the ER.
    Meik Dilcher (1), Subbulakshmi Chidambaram (1), Beate Veith (1), Enno Hartmann (2), Hans-Dieter Schmitt (3), Gabriele Fischer von Mollard (1)
    (1) Biochemistry II, University of Goettingen, Heinrich-Dueker W 12, Goettingen, 37073, Germany (csubbul@gwdg.de); (2) Institut für Biologie, Medizinische Universität zu Lübeck, 23538 Lübeck, Germany; (3) Abteilung Molekulare Genetik, Max-Planck-Institut für biophysikalische Chemie, 37070 Göttingen, Germany

  • 1-20
    •   The Saccharomyces cerevisiae tumour suppressor homologue Sop1p/Sro7p is required for targeting of the sodium transporting ATPase to the cell surface.
    Ingrid Wadskog, Catherine Schowe, Lennart Adler
    Cell and Molecular biology, Göteborg University, Box 462, Göteborg, 405 30, Sweden (ingrid.wadskog@gmm.gu.se)

  • 1-21
    •   t-SNARE phosphorylation and the regulation of Golgi morphology and function.
    Jeffrey Gerst, Adina Weinberger
    Molecular Genetics, Weizmann Institute of Science, Meyer Building, Rehovot, 76100, Israel (jeffrey.gerst@weizmann.ac.il)

  • 1-22
    •   VTC4: a regulator of vacuole size in fission yeast.
    Neil Bone (1), Paul Nurse (2), John Armstrong (1)
    (1) Biological Sciences, Univesity of Sussex, Falmer, Brighton, BN1 9QG, United Kingdom (j.armstrong@sussex.ac.uk); (2) Cancer Research UK, Lincoln's Inn Fields, London, WC2 3PX, UK

  • 1-23
    •   Membrane contact sites between the endocytic and exocytic pathways and non-vesicular lipid traffic.
    Anjana Roy, Tim levine
    Division of Cell Biology, Institute of Ophthalmology, 11-43 Bath St, London, EC1V 9EL, United Kingdom (tim.levine@ucl.ac.uk)

  • 1-24
    •   Sbh1p and the environment of the translocon in the yeast Yarrowia lipolytica.
    Anita Boisramé, Marion Chasles, Anna Babour, Jean-Marie Beckerich, Claude Gaillardin
    Microbiologie et Génétique Mol, Institut National Agronomique, INA, Thiverval-Grignon, 78850, France (beck@grignon.inra.fr)

  • 1-25
    •   The ubiquitin-protein ligase Rsp5p is required to sort membrane proteins into multivesicular bodies.
    J. Morvan, R. Haguenauer-Tsapis, D. Urban-Grimal
    Cell biology, Inst. J. Monod-CNRS-UnivP6-P7, 2, Place Jussieu, Paris Cedex 05, 75251, France (morvan@ijm.jussieu.fr)

  • 1-26
    •   Protein-protein interactions of the PX domain in yeast.
    Carolina S. Vollert, Sebastian Heucke, Peter Uetz
    Institute of Genetics, Forschungszentrum Karlsruhe, PO Box 3640, Karlsruhe, 76021, Germany (carolina.vollert@itg.fzk.de)

  • 1-27
    •   A conserved mechanism targets lipid binding proteins to membrane contact sites between the endoplasmic reticulum and other organelles.
    Christopher JR Loewen, Anjana Roy, Timothy P Levine
    Cell Biology, Institute of Ophthalmology, 11-43 Bath St., London, EC1V 9EL, UK (c.loewen@ucl.ac.uk)

  • 1-28
    •   Mutations in the squalene epoxidase gene of Saccharomyces cerevisiae lead to terbinafine hypersensitivity.
    Friederike Turnowsky, Eva Pitters, Regina Leber, Thomas Kowatz
    IMBM, Karl-Franzens-Universität Graz, Universitätsplatz 2, Graz, A-8010, Austria (friederike.turnowsky@uni-graz.at)

  • 1-29
    •   Pex19p of Hansenula polymorpha.
    Marleen Otzen, Marten Veenhuis, Ida J. van der Klei
    Eukaryotic Microbiology, University of Groningen, Kerklaan 30, Haren, 9750 AA, The Netherlands (M.Otzen@biol.rug.nl)

  • 1-30
    •   Mpp1p, a novel Zn2Cys6 transcription factor essential for peroxisome homeostasis of the methylotrophic yeast Hansenula polymorpha.
    Adriana N. Leao, Arjen M. Krikken, Jan A.K.W. Kiel, Marten Veenhuis
    Eukaryotic Microbiology, University of Groningen, Kerklaan 30, Haren, 9751NN, The Netherlands (a.n.leao@biol.rug.nl)

  • 1-31
    •   Some proteins are degraded preferentially by autophagy under nitrogen starvation.
    Jun Onodera (1), Misuzu Baba (2), Yoshinori Ohsumi (1)
    (1) Department of Cell Biology, National Institute for Basic Biology, and Department of Molecular Biomechanics, School of Life Science, The Graduate University for Advanced Studies, 38 Nishigonaka, Myodajicho, Okazaki 444-8585 Japan (onodera@nibb.ac.jp); (2) Department of Cell Biology, National Institute for Basic Biology, 38 Nishigonaka, Myodajicho, Okazaki 444-8585 Japan

  • 1-32
    •   Bro1, the yeast homologue of the mammalian apoptotic Alix/AIP1 factor, is involved in protein sorting into the multivesicular body (MVB) pathway.
    Elina Nikko, Anne-Marie Marini, Bruno André
    Physiologie cellulaire, Universite Libre de Bruxelles, IBMM, Gosselies, 6041, Belgium (enikko@dbm.ulb.ac.be)

  • 1-33
    •   Paradox of hyper-secretion in a mutant in blocked ER to Golgi transport.
    Pearl Kipnis (1), Peter Lipke (2)
    (1) Biological Sciences, Hunter College of CUNY, 695 Park Avenue, New York, NY 10021, USA (kipnis@genectr.hunter.cuny.edu); (2) (lipke@genectr.hunter.cuny.edu)

  • 1-34
    •   The function of two novel families of metal-ion transporters chaperons.
    Nathan Nelson, Adiel Cohen
    Biochemistry, Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel (nelson@post.tau.ac.il)

  • 1-35
    •   Molecular Characterization of Osh6p: an Oxysterol Binding Protein (OSBP) Homologue in Yeast Saccharomyces cerevisiae.
    Penghua Wang, Han Xiao, Hongzhe Li, Hai Kee Chieu, Hongyuan Yang
    Department of Biochemistry, National Univ of Singapore, 5 Science Drive 2, Sigapore, 119260, Republic of Sigapore (medp1011@nus.edu.sg)

  • 1-36
    •   Role of Sma1p in S.cerevisiae prospore membrane formation.
    Massimiliano Mazza (1), Christian Gunter Riedel (2), Michael Knop (3)
    (1) Cell Biology Program, EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany (mazza@EMBL.de); (2) Research Institute of Molecular Pathology, Gasse 7, 1030 Vienna, Austria; (3) Cell Biology, EMBL Heidelberg, Meyerhofstrasse 1, Heidelberg, 69117, Germany

    Poster Session 2 - Biogenesis and function of mitochondria

  • 2-1
    •   Isolation, heterological cloning and sequencing of the PEL1/PGS1 gene from Kluyveromyces lactis.
    Margita Obernauerova, Silvia Tyciakova, Lucia Dokusova, Kamila Peterkova, Julius Subik
    Dep. Microbiology and Virology, Comenius University, Mlynska dolina B-2, Bratislava, 842 15, Slovak Republic (obernauerova@fns.uniba.sk)

  • 2-2
    •   The special interaction of Gut2p with phosphatidylcholine.
    Pieter Rijken, Marjolein Janssen, Toon de Kroon, Ben de Kruijff
    Biochemistry of Membranes, Institute of Biomembranes, Padualaan 8, Utrecht, NL-3584 CH, The Netherlands (p.j.rijken@chem.uu.nl)

  • 2-3
    •   A genetic approach to identify components involved in phosphatidylethanolamine homeostasis in yeast mitochondria.
    Ruth Nebauer, Ruth Birner, Günther Daum
    Institute of Biochemistry, Technical University Graz, Petersgasse 12/II, Graz, 8010, Austria (ruth.nebauer@tugraz.at)

  • 2-4
    •   Mitochondrial monothiol glutaredoxin Grx5: mechanism of action.
    Jordi Tamarit, Gemma Belli, Elisa Cabiscol, Enrique Herrero, Joaquim Ros
    Ciencies Mediques Basiques, Universitat de Lleida, Rovira Roure 44, Lleida, 25198, Spain (jordi.tamarit@cmb.udl.es)

  • 2-5
    •   Mitochondrial membrane remodelling regulated by a conserved yeast rhomboid.
    G. Angus McQuibban, Matthew Freeman
    Cell Biology, Medical Research Council-LMB, Hills Road, Cambridge, CB2 2QH, United Kingdom (angusm@mrc-lmb.cam.ac.uk)

  • 2-6
    •   Protein insertion into the inner membrane of mitochondria.
    Johannes M. Herrmann (1), Nathalie Bonnefoy (2), Walter Neupert (1), Gregor Szyrach (1)
    (1) Physiological Chemistry, Universitaet Muenchen, Butenandtstrasse 5, Munich, 81377, Germany (hannes.herrmann@bio.med.uni-muenchen.de); (2) Centre de Génétique Moléculaire du CNRS, Avenue de la Terrasse, 91198-Gif sur Yvette, France

  • 2-7
    •   Molecular and genetic approaches to understand the role of COX19, an essential gene for expression of cytochrome oxidase.
    Marina Nobrega (1), Simone Bandeira (1), Alexander Tzagoloff (2)
    (1) IPD-LGMG, Universidade-Vale do Paraiba, Av. Shishima Hifumi, S.J.Campos - SP, 12244000, Brazil (mnobrega@univap.br); (2) Dept.of Biological Sciences, Columbia University, N.Y. 10027, USA

  • 2-8
    •   Linking Mitochondrial Gene Expression and Nuclear Signals Through the Amino-terminal Domain of Mitochodnrial RNA Polymerase.
    Gerald Shadel
    Biochemistry, Emory University School of Med, 1510 Cliften Road NE, Atlanta, GA 30322-3050, USA (gshadel@emory.edu)

  • 2-9
    •   Dependence of mitochondrial protein translation on anionic phospholipids.
    William Dowhan, Xuefeng Su
    Biochem. & Mol. Biol., Univ. of Texas Med. Sch., 6431 Fannin St., Houston, TX 77030, USA (william.dowhan@uth.tmc.edu)

  • 2-10
    •   The mitochondrial Hsp70 Ssc1 is the core-component of an ATP-driven preprotein import motor complex.
    Andreas Strub, Karin Röttgers, Wolfgang Voos
    Institut für Biochemie, University of Freiburg, Hermann-Herder-Str.7, Freiburg, D-79104, Germany (wolfgang.voos@biochemie.uni-freiburg.de)

  • 2-11
    •   Target site cleavage by the homing endonuclease I-SpomI from fission yeast mitochondria.
    Stefan Pellenz (1), Bernard Dujon (1), Bernd Schäfer (2)
    (1) Unité de Génétique Moléculaire des Levures (URA 2171/CNRS and UFR927/Univ. P. M. Curie), Institut Pasteur, 25 Rue du Dr. Roux, F-75724 Paris-CEDEX 15, France (pellenz@pasteur.fr); (2) Institut für Biologie IV (Mikrobiologie), Rheinisch Westfälische Technische Hochschule Aachen, Worringer Weg 1, D-52056 Aachen, Germany, Rheinisch Westfälische Technische Hochschule Aachen, D-52056 Aachen, Germany

  • 2-12
    •   Tar1p, a novel protein encoded in the nuclear rDNA repeat: a new link between the nucleus and mitochondria.
    Nicholas D. Bonawitz, Anthony C. Bryan, Christopher M. Wearn, Gerald S. Shadel
    Department of Biochemistry, Emory University, 1510 Clifton Road, Atlanta, GA 30322, USA (nbonawi@emory.edu)

  • 2-13
    •   Characterization of mitochondrial tRNA mutants of Saccharomyces cerevisiae.
    Cristina De Luca, Lorella D'Amico, Teresa Colombo, Silvia Francisci, Laura Frontali
    Developmental and Cell Biology, University of Rome1, P.ale A. Moro 5, Rome, I00185, Italy (Cristina.DeLuca@uniroma1.it)

  • 2-14
    •   Yeast mitochondrial kinome: From in silico to in vivo localization.
    Lubomir Tomaska (1), Peter Rohac (2), Blanka Kucejova (2), Jozef Nosek (2), H. Yde Steensma (3)
    (1) Department of Genetics, Comenius University, Faculty of Natural Sciences, Mlynska dolina B-1, 84215 Bratislava, Slovakia (tomaska@fns.uniba.sk); (2) Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 84215 Bratislava, Slovakia; (3) Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratorium, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

  • 2-15
    •   Genetic analysis of a preferential action of ionophores on yeast mitochondria.
    Blanka Kucejova (1), Silvia Petrezselyova (2), Jozef Nosek (1), Lubomir Tomaska (2)
    (1) Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 84215 Bratislava, Slovakia (kucejova@fns.uniba.sk); (2) Department of Genetics, Comenius University, Faculty of Natural Sciences, Mlynska dolina B-1, 84215 Bratislava, Slovakia

  • 2-16
    •   Linear mitochondrial genomes: An intraspecific variability of mitochondrial telomeres in Candida parapsilosis.
    Jozef Nosek (1), Adriana Rycovska (2), Matus Valach (2), Lubomir Tomaska (3)
    (1) Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovakia; Institute of Genetics and Microbiology, University of Paris XI, 91405 Orsay Cedex, France; (2) Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovakia (nosek@fns.uniba.sk); (3) Department of Genetics, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovakia

  • 2-17
    •   The yeast cAMP protein kinase TPK3 is involved in mitochondrial biogenesis.
    Anne Devin, Julie Vallortigara, Michel Rigoulet
    Cell energetic metabolism, IBGC du CNRS, 1 rue C. Saint Saens, bordeaux, 33077, France (anne.devin@ibgc.u-bordeaux2.fr)

  • 2-18
    •   A role of Mhr1p-dependent DNA recombination for the formation of homoplasmy in yeast mitochondria.
    Feng Ling, Takehiko Shibata
    Cellular & Molecular Biology L, The Institute of Physical and 2-1 Hirosawa, Wako, 351-0198, Saitama, Japan (ling@postman.riken.go.jp)

  • 2-19
    •   Endogenous substrates-recognition and degradation by the yeast Lon protease.
    Katarina Slezakova (1), Gabriela Durcova (1), Dusan Perecko (1), Natalia Parkhomenko (2), Jiri Janata (2), Eva Kutejova (1)
    (1) Department of Enzymology, Institute of Molecular Biology, Dubravska cesta 21, Bratislava, 845 51, Slovak Republic (slezakova@hotmail.com); (2) Institute of Microbiology, Czech Academy of Sciences, Videnska 1083, 142 20 Praha 4, Czech Republic

  • 2-20
    •   Mitochondrial intramembrane peptidases.
    Karlheinz Esser (1), Martin Ingenhoven (1), Baris Tursun (2), Georg Michaelis (1), Elke Pratje (2)
    (1) Botany, University of Duesseldorf, Universitaetsstr. 1, Duesseldorf, D-40225, Germany (esserkh@uni-duesseldorf.de); (2) Institut fuer Allgemeine Botanik, Ohnhorststr. 18, D-22609 Hamburg, Germany

  • 2-21
    •   Overproduction of Cox2p prevents synthesis of Cox1p and respiratory growth.
    Alessandro Fiori, Xochitl Perez-Martinez, Thomas D. Fox
    Molecular Biology and Genetics, Cornell University, Tower Road, Ithaca, NY 14850, USA (af83@cornell.edu)

  • 2-22
    •   Mss51p acts in two different and independent regions of the mitochondrial COX1 mRNA to promote its translation.
    Xochitl Perez-Martinez, Sarah A. Broadley, Thomas D. Fox
    Molecular Biology and Genetics, Cornell University, 333 Biotech. Bldg., Ithaca, NY 14853, USA (xp24@cornell.edu)

  • 2-23
    •   The small Tim complexes escort mitochondrial inner membrane proteins across the intermembrane space.
    Carla M Koehler, Sean P. Curran, Danielle Leuenberger
    Chemistry and Biochemistry, UCLA, 607 Young Drive East, Los Angeles, CA 90095-1569, USA (koehler@chem.ucla.edu)

  • 2-24
    •   Functional dissection of the Oxa1 translocase.
    Soledad Funes, Walter Neupert, Johannes M. Herrmann
    Physiologische Chemie, LMU Muenchen, Butenandtstrasse 5, Muenchen, 81377, Germany (Soledad.Funes@bio.med.uni-muenchen.de)

  • 2-25
    •   Rpm2p, a component of mitochondrial RNase P, acts as a transcriptional activator in the nucleus.
    Vilius Stribinskis, Steve Ellis, Nancy Martin
    Biochemistry & Molecular Biol., University of Louisville, 319 Abraham Flexner, Louisville, KY 40292, USA (v0stri01@louisville.edu)

  • 2-26
    •   Identification and characterization of Tim50p, a novel component of the mitochondrial TIM23 preprotein translocase.
    Dejana Mokranjac (1), Stefan A. Paschen (1), Holger Prokisch (1), Suzanne C. Hoppins (2), Frank E. Nargang (2), Walter Neupert (1), Kai Hell (1)
    (1) Physiological Chemistry, University of Munich, Butenandtstr. 5, Muenchen, 81377, Germany (hell@bio.med.uni-muenchen.de); (2) Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada

  • 2-27
    •   Comparative mitochondrial genomics and gene expression in the Monoblepharidales.
    Charles Bullerwell, Jessica Leigh, B. Franz Lang
    Biochemistry, Universite de Montreal, 2900, Ed. Montpetit, Montreal, H3C 3J7, Canada (Franz.Lang@Umontreal.ca)

  • 2-28
    •   Expression of the maize mitochondrial URF13 represses galactose-induced Gal1 gene expression in the yeast S. cerevisiae.
    Hamza El-Dorry, Felipe Chambergo, Lia Muschellack, Robert Schumacher, José Ribamar Ferreira Jr.
    Institute of Chemistry, University of São Paulo, Lineu Prestes 748, São Paulo, 05508-900, Brazil (dorry@iq.usp.br)

  • 2-29
    •   Yol027/yLetM1 encodes a mitochondrial protein involved in the control of membrane potential and organelle volume.
    Karin Nowikowski, Gabor Zsurka, Martin Kolisek, Ludmilla Zotova, Rudolf Schweyen
    Microbiology and Genetics, University of Vienna, Dr. Bohrgasse 9, Vienna, A-1030, Austria (rudolf.schweyen@univie.ac.at)

  • 2-30
    •   D-lactate transport and metabolism in isolated Saccharomyces cerevisiae mitochondria.
    Maria Luigia Pallotta, Daniela Valenti, Michelina Iacovino, Salvatore Passarella
    S.A.V.A., University of Molise, Via de Sanctis, Campobasso, 86100, Italy (pallotta@unimol.it)

  • 2-31
    •   Identification of the mitochondrial hydroxyacyl-ACP dehydratase.
    Alexander J. Kastaniotis, Kaija Autio, Raija Sormunen, J. Kalervo Hiltunen
    Department of Biochemistry, University of Oulu, Linnanmaa Campus, Oulu, 90570, Finland (akastani@sun3.oulu.fi)

  • 2-32
    •   A heritable structural alteration of the yeast mitochondrion.
    Daniel Lockshon
    Biochemistry, University of Washington, Box 357350, Seattle, WA 98195, USA (lockshon@u.washington.edu)

  • 2-33
    •   Role of the Hmi1 helicase in mtDNA metabolism.
    Tiina Sedman, Priit Jõers, Silja Kuusk, Juhan Sedman
    Department of Biochemistry, University of Tartu, Vanemuise 46, Tartu, 51014, Estonia (jsedman@ebc.ee)

    Poster Session 3 - Shaping the cell: morphogenesis, cytoskeleton, polarity and cell wall

  • 3-1
    •   Identification of cell surface determinants in Candida albicans.
    Constantin Urban (1), Kai Sohn (1), Friedrich Lottspeich (2), Michael Schweikert (3), Herwig Brunner (1), Steffen Rupp (1)
    (1) IGB, Fraunhofer, Nobelstrasse 12, Stuttgart, 70569, Germany (cur@igb.fhg.de); (2) Max Planck Institute of Biochemistry, Am Klopferspitz, Martinsried 82152, Germany; (3) University of Stuttgart, Department of Zoology, Biological Institute, Am Pfaffenwaldring, Stuttgart 70550, Germany

  • 3-2
    •   Identification of cell wall proteins of the fungal pathogen Candida albicans and other fungi using mass-spectrometric and genome-wide computational approaches.
    Piet W.J. De Groot (1), Henk L. Dekker (2), Albert D. De Boer (1), Klaas J. Hellingwerf (1), Chris G. De Koster (2), Frans M. Klis (1)
    (1) Laboratory for Microbiology, University of Amsterdam, Nieuwe Achtergracht, Amsterdam, 1018 WV, The Netherlands (pgroot@science.uva.nl); (2) Mass Spectrometry Group, SILS-UvA, Amsterdam, The Netherlands

  • 3-3
    •   Homologs of the GPI-anchored beta(1,3)-Glucan transferase Gas1p of Saccharomyces cerevisiae have specific roles during sporulation.
    Enrico Ragni (1), Cristina Carotti (1), Marina Vai (2), Laura Popolo (1)
    (1) Fisiologia e Biochimica Gen, Università di Milano, Via Celoria 26, Milano, 20133, Italy (Enrico.Ragni@unimi.it); (2) Università di Milano - Bicocca, P.le delle Scienze 2 - Milano Italy

  • 3-4
    •   Interaction of Rpg1p with Sla2p in Saccharomyces cerevisiae.
    Filip Janda, Ivana Janatova, Alena Jiraskova, Katerina Malinska, Jiri Hasek
    Lab. of Cell Reproduction, Institute of Microbiology, Videnska 1083, Prague 4, 142 20, Czech Republic (jandaf@biomed.cas.cz)

  • 3-5
    •   Mutation of the PMT1 gene encoding O-mannosyltransferase causes severe defects of the cell-wall structure in Hansenula polymorpha.
    Svyatoslav Sokolov (1), Michael Agafonov (2), Natalya Suzina (3), Tatyana Kalebina (1)
    (1) Molecular Biology, Moscow State University, Vorobevy gory, Moscow, 119992, Russia (Sokolov@protein.bio.msu.ru); (2) Cardiology Research Center, 3rd Cherepkovskaya str. 15A, Moscow, 121552 Russia; (3) Institute of Biochemistry and Physiology of Microorganisms. Pushchino, 142290 Russia

  • 3-6
    •   Deletion of ISW2 induces alpha-mating type-specific invasive growth in S. cerevisiae.
    Ivana Frydlova, Petra Trachtulcova, Ivana Janatova, Jiri Hasek
    Lab. of Cell Reproduction, Institute of Microbiology ASCR, Videnska 1083, Prague 4, 142 20, Czech Republic (frydlova@biomed.cas.cz)

  • 3-7
    •   Interaction between a Ras-family and Rho-family GTPase couples selection of a growth site to the development of cell polarity.
    Keith G. Kozminski (1), Laure Beven (2), Elizabeth Angerman (2), Amy Tong (3), Charles Boone (3), Ira Herskowitz (4), Hay-Oak Park (2)
    (1) Depts of Biology & Cell Biology, University of Virginia, PO Box 400328, Charlottesville, VA 22904, USA (kkoz@virginia.edu); (2) Dept. of Molecular Genetics, Ohio State University, Columbus, OH 43210, USA; (3) Banting & Best Dept. of Medical Research, University of Toronto, Toronto, ON M5S 1A8, Canada; (4) Dept. of Biochemistry & Biophysics, University of California, San Francisco, CA 94143, USA

  • 3-8
    •   Mobility and polarization of plasma membrane proteins in Saccharomyces cerevisiae.
    Javier Valdez, Hugh Pelham
    Cell Biology, MRC Lab. of Molecular Biology, Hills Road, Cambridge, CB2 2QH, United Kingdom (jvaldez@mrc-lmb.cam.ac.uk)

  • 3-9
    •   Gyf1, a novel filamentous protein in yeast.
    Alexander G. Georgiev, Jean-Marc Berrez, Ann Mutvei
    Dept. of Natural Sciences, Södertörns högskola, Alfred Nobels allé 3, Huddinge, 14189, Sweden (alexander.georgiev@sh.se)

  • 3-10
    •   The deletions of the ISW2 and ITC1 genes cause changes in the cell morphology and a defect of the cell wall integrity.
    Petra Trachtulcova, Ivana Frydlova, Ivana Janatova, Jiri Hasek
    Lab. Cell Reproduction, Institute of Microbiology, Videnska 1083, Prague, 14220, Czech Republic (petra@biomed.cas.cz)

  • 3-11
    •   Structural and functional analysis of Bud8p and Bud9p.
    Anne-Brit Obermayer, Hans-Ulrich Mösch
    Mol. Microbiology and Genetics, Georg-August-University, Grisebachstr. 8, Göttingen, D-37077, Germany (aoberma1@gwdg.de)

  • 3-12
    •   The cell polarity kinase Ssp1 acts through Cmk3 kinase to control mitosis onset.
    Sandra López-Avilés, Maribel Grande, Marta González, Oriol Bachs, Rosa Aligue
    Cell Biology, University of Barcelona, C/ Casanova 143, Barcelona, 08036, Spain (slopez@medicina.ub.es)

  • 3-13
    •   Characterization of new cdc24 yeast mating mutants.
    Sophie Barale, Robert A. Arkowitz
    Signaling, Dev. Biol. & Cancer, CNRS UMR6543, Parc Valrose, Nice, 06108, France (arkowitz@unice.fr)

  • 3-14
    •   A meiotic lipid signaling system regulating nuclear divisions and spore formation in budding yeast.
    Gela Tevzadze, Jessica Pierce, Rochelle Easton Esposito
    Mol Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA (getevzad@midway.uchicago.edu)

  • 3-15
    •   Characteristics of the transcriptional response to cell wall stress in Saccharomyces cerevisiae.
    André Boorsma (1), Hans de Nobel (2), Bas ter Riet (1), Bastiaan Bargmann (1), Stanley Brul (1), Klaas J Hellingwerf (1), Frans M Klis (1)
    (1) SILS-Microbiology, University of Amsterdam, Nwe achtergracht 166, Amsterdam, 1018 WV, Netherlands (boorsma@science.uva.nl); (2) R&D Gene Discovery, Genencor International B.V., Postbus 218,2300 AE Leiden, Netherlands

  • 3-16
    •   Functional inter-dependence between yeast cis-prenyltransferases.
    Kariona Grabinska (1), Grazyna Sosinska (1), Ewa Swiezewska (2), Thierry Berges (3), Francis Karst (3), Grazyna Palamarczyk (1)
    (1) Lab. of Fungal Glycobiology, IBB PAS, Pawinskiego 5a, Warsaw, 02-106, Poland (grazynas@ibb.waw.pl); (2) Department of Lipid Biochemistry, IBB PAS; (3) Department of Yeast Genetics, University of Poitiers, France

  • 3-17
    •   RAM: a conserved signaling network that regulates Ace2p transcription factor and polarized morphogenesis.
    Bryce Nelson (1), Cornelia Kurischko (2), Joe Horecka (3), Manali Mody (2), Pradeep Nair, Lana Pratt (4), Timothy Hughes (1), Charlie Boone (1), Frank Luca (2)
    (1) Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada (fluca@vet.upenn.edu); (2) Department of Animal Biology, University of Pennsylvania, 3800 Spruce St., Philadelphia, PA 19104, USA; (3) DiscoveRx Corp., Freemont, CA 94538; (4) Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada

  • 3-18
    •   Regulation of apical growth during hyphal development in Candida albicans.
    Idit Hazan, Haoping Liu
    Biological Chemistry, UC Irvine, 19172 Jambaree Rd., Irvine, CA 97267-1700, USA (h4liu@uic.edu)

  • 3-19
    •   Scaffold-mediated symmetry breaking by Cdc42p.
    Javier Irazoqui, Daniel Lew
    Pharmacology and Cancer Bgy., Duke University Medical Center, PO Box 3813, Durham, NC 27710, USA (jei@duke.edu)

  • 3-20
    •   Inhibition of yeast morphogenesis and actin polarization by the Salmonella typhimurium phosphatydilinositol phosphatase SigD.
    Isabel Rodríguez-Escudero, Humberto Martín, César Nombela, Rafael Rotger, Victor J. Cid, María Molina
    Microbiología II Farmacia, Universidad Complutense, Pza Ramón y Cajal, Madrid, 28040, Spain (humberto@farm.ucm.es)

  • 3-21
    •   SH3 domain containing proteins in the organization, signalling and motility of the actin cytoskeleton; a proteomic approach.
    Alexandre Soulard (1), Vladyslav Spiridonov (1), Serena Paoluzi (2), Gianni Cesareni (2), Barbara Winsor (1)
    (1) MLPH, IBMC du CNRS, 15 rue Descartes, Strasbourg, 67084, France (B.Winsor@ibmc.u-strasbg.fr); (2) Dept of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica, 00133 Rome, Italy

  • 3-22
    •   Formin polarity in Ashbya gossypii.
    Hans-Peter Schmitz, Andreas Kaufmann, Michael Köhli, Peter Philippsen
    Applied Microbiology, Biozentrum University of Basel, Klingelbergstr. 50, Basel, 4056, Switzerland (h-p.schmitz@unibas.ch)

  • 3-23
    •   A non-annotated ORF, LME1, defines a new biochemical entity with potential involvement in the cell cycle.
    Elena Kisseleva-Romanova, Abdelkader Namane, Agnés Baudin-Baillieu, Carl Mann, Domenico Libri
    RNA, CGM-CNRS, av. de la Terrasse, Gif-sur-yvette, 91190, France (lena@cgm.cnrs-gif.fr)

  • 3-24
    •   Is there a role for communication between the nucleus and the cleavage apparatus in the assembly, maturation and/or function of the cytokinetic machinery?
    Caren Norden, Yves Barral
    Biology, Biochemistry, ETH Hoenggerberg, Zurich, 8093, Switzerland (Caren.Norden@bc.biol.ethz.ch)

  • 3-25
    •   Kar9 dependent guidance of a subclass of microtubules ensures proper spindle orientation in yeast.
    Justine Kusch, Yves Barral
    Biology, Biochemistry, ETH Hoenggerberg, Zurich, 8093, Switzerland (justine.kusch@bc.biol.ethz.ch)

  • 3-26
    •   GTPase activity and filament assembly of yeast septins.
    Marian Farkasovsky, Peter Herter, Alfred Wittinghofer
    Structural Biology, MPI for Molecular Physiology, Otto-Hahn-Strasse 11, Dortmund, 44227, Germany (marian.farkasovsky@mpi-dortmund.mpg.de)

  • 3-27
    •   CRH1 is involved in the S. cerevisiae compensatory response to cell wall damage.
    Estefanía Rodriguez, Jose Manuel Rodriguez, Raul García, Clara Bermejo, Gema Díaz, Rosa Pérez, Carmen Rivas, César Nombela, Javier Arroyo
    Microbiología II, Universidad Complutense, Fac. de Farmacia, Madrid, 28040, Spain (jarroyo@farm.ucm.es)

  • 3-28
    •   The role of F-actin in Cdc42p polarization.
    Javier E. Irazoqui, Daniel J. Lew
    Pharm. & Cancer Biol., Duke University Med Ctr, Research Dr., Durham, NC 27710, USA (daniel.lew@duke.edu)

  • 3-29
    •   Actin Cytoskeleton Is Required For Nuclear Localization Of Gln3 During Nitrogen Limitation in Saccharomyces cerevisiae.
    Kathleen H. Cox, Jennifer J. Tate, Terrance G. Cooper
    Molecular Sciences, University of Tennessee, 858 Madison Ave., Memphis, TN 38163, USA (khcox@utmem.edu)

  • 3-30
    •   Role(s) of Septins in Cytokinesis.
    Jeroen Dobbelaere, Yves Barral
    ETH Zürich, Biochemistry, Schafmattstrasse, Zürich, 8093, Switzerland (jeroen.dobbelaere@bc.biol.ethz.ch)

  • 3-32
    •   Dynamics of actin patch assembly in Saccharomyces cerevisiae.
    Marko Kaksonen, David Drubin
    Molecular and Cell Biology, UC Berkeley, 401 Barker Hall, Berkeley, CA 94720, USA (kaksonen@uclink.berkeley.edu)

  • 3-33
    •   Regulation and role of the flocculation-inducing protein, Fip1, in asexual flocculation of fission yeast.
    Yoon Dong Park (1), Wan-Soo Yang (1), Eun-Kyung Kim (1), Kyung-Sook Bae (2), Hee-Moon Park (1)
    (1) Microbiology, Chungnam National University, Yuseong-gu, Daejeon, 305-764, South Korea (hmpark@cnu.ac.kr); (2) KCTC, Korea Research Institiute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, South Korea

  • 3-34
    •   Subcellular localization of Dcw1, a GPI-anchored membrane protein involved in the cell wall synthesis.
    Hitoshi Shimoi, Hiroshi Kitagaki, Kiyoshi Ito
    Genetic Engineering Division, Research Institute of Brewing, 3-7-1, Kagamiyam, Higashihiroshima, 739-0046, Japan (simoi@nrib.go.jp)

  • 3-35
    •   Structural inheritance: the role of inherited structural elements in the polarisation of the fission-yeast cell.
    Matthias Sipiczki, Ida Miklos
    Department of Genetics, University of Debrecen, Hungary; Research Group of Microbial Development, Hungarian Academy of Sciences, Debrecen, Hungary (lipovy@tigris.klte.hu)

  • 3-36
    •   spl1-1 : a cytokinesis defect caused by a G-to-A transition in the D-loop of a proline-tRNA of S. pombe.
    Ida Miklos, M. Sipiczki
    Department of Genetics, University of Debrecen, Debrecen, Hungary; Research Group of Microbial Development, Hungarian Academy of Sciences, Debrecen, Hungary (lipovy@tigris.klte.hu)

  • 3-37
    •   Investigation of the mode of action of Mid2p.
    Gordon Ford, Katherine Wilson, Doug Stirling
    Division of Cell Signalling, Faculty of Life Sciences, University of Dundee, Dundee, DD1 5EH, United Kingdom (g.z.ford@dundee.ac.uk)

  • 3-38
    •   Nak1, a GC kinase is essential for growth and bipolar morphology in fission yeast.
    Timothy Huang, Dallan Young
    Biochemistry & Molecular Biol, University of Calgary, 3330 Hosptial Dr NW, Calgary, AB T2N4N1, Canada (young@ucalgary.ca)

  • 3-39
    •   The VGA3 gene from Kluyveromyces lactis affects secretion of endogenous and heterologous proteins as well as cell wall properties.
    Silvia Rufini (1), Daniela Uccelletti (1), Francesca Farina (1), Claudia Abeijon (2), Claudio Palleschi (1)
    (1) Developmental and Cell Biology, University La Sapienza, P.le Aldo Moro, 5, 00185, Rome, Italy (silvia.rufini@uniroma1.it); (2) Dpt. Molecular and Cell Biology, Boston University, Boston, MA USA.

  • 3-40
    •   The cell polarity factor, Tea1, is a potential downstream target of the Ste20/PAK homolog, Shk1, in the fission yeast, Schizosaccharomyces pombe.
    Peirong Yang, HyeWon Kim, Stevan Marcus
    Dept. of Molecular Genetics, UT M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA (smarcus@mdacc.tmc.edu)

    Poster Session 4 - Filamentous growth and pathogenesis and pseudohyphal growth

  • 4-1
    •   Investigation of the potential role of Ace2p as a virulence factor in Candida albicans.
    Mary. T Kelly (1), Frank Odds (2), Geraldine Butler (1)
    (1) Dept. of Biochemistry, DMMC University College Dublin, Merville House, Belfield, Dublin, 04, Ireland (Mary.T.Kelly@ucd.ie); (2) Dept. of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, AB25 2ZD, Scotland, United Kingdom

  • 4-2
    •   Deletion of CaPDE2, the gene encoding high-affinity cAMP phosphodiesterase, alters antifungal drug susceptibility in Candida albicans.
    Won Hee Jung, Lubomira I. Stateva
    Biomolecular Sciences, UMIST, P.O.Box 88, Manchester, M60 1QD, U.K. (W.Jung@stud.umist.ac.uk)

  • 4-3
    •   Decrease in the amount of translation termination factor eRF1 inhibits philamentous growth in Saccharomyces cerevisiae.
    Denis Kiktev (1), Svetlana Moskalenko (1), Michel Philippe (2), Galina Zhouravleva (1)
    (1) Department of Genetics, St. Petersburg State University, Universitetskaya nab, St. Petersburg, 199034, Russia (denis@btc.bio.pu.ru); (2) Université de Rennes 1, CNRS UMR 6061, IFR 97, 2 av. Pr. Léon Bernard 35043 Rennes Cedex, France

  • 4-4
    •   Identification of Trans-Regulatory Factors of the Efflux Pumps Involved in Drug Resistance in Candida albicans.
    Chia Geun Chen (1), Hsin I Shih (2), Yun Liang Yang (2), Hsiu Jung Lo (1)
    (1) Division of Clinical Research, National Health Research Inst., 128 Yen-Chiu-Yuan Rd, Taipei, 11529, Taiwan, R.O.C. (jugen@nhri.org.tw ); (2) Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, R.O.C.

  • 4-5
    •   Rme1p induces FLO11 expression through an 11 bp Rme1p response element.
    Dewald van Dyk, Florian F. Bauer, Isak S. Pretorius
    Inst. for Wine Biotechnology, Stellenbosch University, Victoria Street, Stellenbosch, 7600, South Africa (iwbt22@sun.ac.za)

  • 4-6
    •   Signalling pathways controlling the expression of hyphae-associated secreted aspartic proteinases of Candida albicans during infection.
    Peter Staib (1), Ayfer Binder (1), Marianne Kretschmar (2), Thomas Nichterlein (2), Joachim Morschhäuser (1)
    (1) Institut für Molekulare Infektionsbiologie, Universität Würzburg, Germany (joachim.morschhaeuser@mail.uni-wuerzburg); (2) Institut für Medizinische Mikrobiologie und Hygiene, Universität Heidelberg, Germany

  • 4-7
    •   The GTPase module Cdc24/Cdc42 is required for invasive growth of Candida albicans.
    Martine Bassilana, James Blyth, Robert Arkowitz
    Centre de Biochimie. UMR 6543, CNRS, Parc Valrose, Nice, 06108, France (mbassila@unice.fr)

  • 4-8
    •   Pathogenic versus non-pathogenic ascomycetes: characterisation of CaGEF1 and CaPLC2/3 in C. albicans that do not have an orthologue in S. cerevisiae.
    Philipp Knechtle (1), Sophie Brachat (2), Christophe d'Enfert (1)
    (1) Fungal Biol & Patho, Institut Pasteur, 25, R Docteur Roux, Paris, 75724, France (knechtle@pasteur.fr); (2) Applied Microbiology, Biozentrum der Universitaet Basel, Klingelbergstrasse 70,4056 Basel, Switzerland

  • 4-9
    •   In vitro reconstructed human epithelia reveal contributions of C. albicans EFG1 and CPH1 to adhesion and invasion.
    Christoph Dieterich (1), Markus Schandar (2), Franz-Josef Johannes (2), Herwig Brunner (2), Hans-Georg Eckert (2), Steffen Rupp (2)
    (1) Max-Planck-Institute for Molecular Genetics Computational Biology Group, Ihnestrasse 73; 14195 Berlin; Germany; (2) NWG I, Fraunhofer IGB, Nobelstr. 12, Stuttgart, 70569, Germany (rupp@igb.fhg.de)

  • 4-10
    •   A screen in S. cerevisiae identified CaMCM1, an essential gene in C. albicans crucial for morphogenesis.
    Matthias Rottmann, Marija Dukalska, Sonja Dieter, Herwig Brunner, Steffen Rupp
    NWG I, Fraunhofer IGB, Nobelstr. 12, Stuttgart, 70569, Germany (rupp@igb.fhg.de)

  • 4-11
    •   FLO11 is required for biofilm formation on a liquid surface by Saccharomyces cerevisiae.
    Severino Zara (1), Giacomo Zara (1), Alan T. Bakalinsky (2), Giorgia Pirino (1), Marilena Budroni (1)
    (1) DISAABA, Sez. Microbiologia, University of Sassari, Viale Italia 39, Sassari, 07100, Italy (szara@uniss.it); (2) Department of Food Science and Technology, Oregon State University, Corvallis, Oregon 97331-6602, USA

  • 4-12
    •   The LAG1 homologous genes are required for true hypha formation in Yarrowia lipolytica and Candida albicans.
    Seon Ah Cheon (1), Yunkyoung Song (1), Byung Kyu Lee (2), Heuil Kang (2), Jeong-Yoon Kim (1)
    (1) Microbiology, Chungnam National University, Yuseong, Daejeon, 305-764, Korea (sacheon@cnu.ac.kr); (2) Yuhan Research Institute, Gyeonggi-do, Korea

  • 4-13
    •   Schizosaccharomyces pombe : A Dimorphic Fission Yeast.
    Evelyn Amoah-Buahin, Ramona Ramdath, Thalia Antoniadi, Neil Bone, John Armstrong
    School of Biological Sciences, University of Sussex, Falmer, Brighton, Sussex, BN1 9QG, United Kingdom (e.amoah-Buahin@sussex.ac.uk)

  • 4-14
    •   A new class of heterotrimeric G protein governs yeast pseudohyphal differentiation.
    Toshiaki Harashima, Joseph Heitman
    Mol. Genetics and Microbiology, DUMC, HHMI, Research Drive, Durham, NC 27710, USA (t0084h@acpub.duke.edu)

  • 4-15
    •   Functional characterization of oxysterol-binding proteins in budding yeast Saccharomyces cerevisiae and pathogenic yeast Candida albicans.
    Ji-ho Ryu, Kwang-hoon Kim, Hyangsuk Huh, Jinmi Kim
    Microbiology, Chungnam National University, KungDong 220, Taejeon, 305-764, South Korea (jmkim@cnu.ac.kr)

  • 4-16
    •   Perception and transport of plant hormones in yeast.
    Reeta Prusty, Paula Grisafi, Gerry Fink
    Whitehead Institute, MIT, 9 Cambridge Center, Cambridge, MA 02142, USA (prusty@wi.mit.edu)

  • 4-17
    •   Mos10 (Vps60) is required for normal filament maturation in S. cerevisiae.
    Julia R. Köhler
    Division of Infectious Disease, Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA (julia.koehler@tch.harvard.edu)

  • 4-18
    •   Regulation of the Hyphal Transcription Program in Candida albicans.
    Prashna Pala Raniga, Maithili Shrivastava, Haoping Liu
    Biological Chemistry, UC Irvine, 19182 Jamboree Blvd, Irvine, CA 92697, USA (prashnapala@hotmail.com)

  • 4-19
    •   C. albicans requires high capacity amino acid uptake for virulent growth.
    Paula Martínez, Per O. Ljungdahl
    Yeast Molecular Biology, Ludwig Inst. Cancer Research, Box 240, Stockholm, 171 77, Sweden (pmar@licr.ki.se)

    Poster Session 5 - Nuclear structure, function and transport

  • 5-1
    •   Characterization of Nep2, a polytopic nuclear membrane protein.
    Arunas Leipus (1), Jean-Marc Berrez (2), Ann Mutvei-Berrez (2)
    (1) Department of Biochemistry, Umeå University, University Campus, Umeå, S-90187, Sweden (Arunas.Leipus@chem.umu.se); (2) Department of Natural Sciences, Södertörns University College, S-141 89 Huddinge, Sweden

  • 5-2
    •   Loss of Orc2 leads to impairment of an S phase checkpoint and induction of G2/M delay.
    Kenji Shimada (1), Philippe Pasero (2), Susan Gasser (1)
    (1) Dep. of Molecular Biology, University of Geneva, 30 Quai E-Ansermet, Geneva 4, 1211, Switzerland (Kenji.Shimada@molbio.unige.ch); (2) Institute of Molecular Genetics, Unité Mixte de Recherche 5535, CNRS, F-34033, Montpellier, France

  • 5-3
    •   The perinuclear Mlp proteins contribute to a quality control step of yeast mRNP complexes prior to nuclear export.
    Patrizia Vinciguerra, Daniel Zenklusen, Nahid Iglesias, Françoise Stutz
    Cell Biology, University of Geneva, 30 Quai E. Ansermet, Geneva 4, 1211, Switzerland (Francoise.Stutz@cellbio.unige.ch)

  • 5-4
    •   The cytoplasmic localization of the tRNA aminoacylation cofactor Arc1p depends on Xpo1p-mediated nuclear export.
    Kyriaki Galani (1), Eduard Hurt (1), George Simos (2)
    (1) Biochemie-Zentrum Heidelberg (BZH), Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany; (2) Laboratory of Biochemistry, Medical School, Univ. Thessaly, Papakyriazi 22, Larissa, GR-41222, Greece (simos@med.uth.gr)

  • 5-5
    •   Mutants of the SUMO modification system affect a specific nuclear protein import pathway in yeast.
    Katrin Stade (1), Frank Vogel (1), R. Jürgen Dohmen (2), Thomas Sommer (1)
    (1) Cell Biology Dept., Max-Delbrück-Center (MDC), Robert-Rössle-Str.10, Berlin, D-13092, Germany (kstade@mdc-berlin.de); (2) University of Cologne, Institute for Genetics, Zülpicher Str. 47, D-50674 Köln, Germany

  • 5-6
    •   Subcellular localization and characterization of the arginine methyltransferase Rmt2.
    Ida Olsson (1), Jean-Marc Berrez (1), Arunas Leipus (2), Ann Mutvei (1)
    (1) Department of Natural Scenices, Södertörns Högskola, Alfred Nobels allé 7, Huddinge, 141 89, Sweden (ida.olsson@sh.se); (2) Department of Biochemistry, Umeå University, Umeå, Sweden

  • 5-7
    •   Linker histone H1 does have a role in Saccharomyces cerevisiae chromatin structure.
    Milena Kirilova, George Miloshev
    Department Molecular Genetics, Institute of Molecular Biology, Acad. G. Bonchev 21, Sofia, 1113, Bulgaria (gam@obzor.bio21.bas.bg)

  • 5-8
    •   Coupling Transcription to Nuclear mRNA Export.
    Katja Straesser (1), Ed Hurt (2)
    (1) Gene Center, University of Munich (LMB), Feodor-Lynen-Str. 25, Munich, 81377, Germany (strasser@lmb.uni-muenchen.de); (2) Im Neuenheimer Feld 328, 69120 Heidelberg, Germany

  • 5-9
    •   In vivo interactions of nucleocytoplasmatic transport factors in yeast.
    Isabell Lobert, Iris Zengerly, Gabriel Schlenstedt
    Universität des Saarlandes, Med.Biochemie/Molekularbiolog., Gebäude 44, Homburg, D-66421, Germany (bcizen@uniklinik-saarland.de)

  • 5-10
    •   DNA damage response mediated degradation of Ho via the proteasome involves its nuclear export.
    Yelena Ivantsiv, Ludmila Kaplun, Anya Bakhrat, Dina Raveh
    Life Sciences, Ben Gurion University, P.O. Box 653, Beersheba, 84105, Israel (raveh@bgumail.bgu.ac.il)

  • 5-11
    •   Nuclear import of yeast Gcn4p requires karyopherins Srp1p and Kap95p.
    Katrin Bömeke (1), Ralph Pries (1), Oliver Draht (1), Markus Künzler (2), Gerhard Braus (1)
    (1) University Göttingen, Molecular Microbiology, Grisebachstraße 8, Göttingen, D-37077, Germany (kboemek@gwdg.de); (2) Institute of Microbiology, ETH-Zentrum LFV E22, Schmelzbergstraße 7, CH-8092 Zürich, Swizerland

  • 5-12
    •   Rpb4p, a subunit of RNA polymerase II, mediates mRNA export during stress.
    Mordechai Choder (1), Marganit Farago (2), Tal Nahari (2), Christopher Hammel (3), Charle N. Cole (3)
    (1) Microbiology, Technion, Bat-Galim, Haifa, 31096, Israel (choder@tx.technion.ac.il); (2) Department of Molecular Microbiology & Biotechnology, Tel-Aviv Univ. Israel; (3) Dartmouth Med. School, Hanover NH, USA

  • 5-13
    •   Mlp proteins regulate poly(A) RNA export from the nucleus via interactions with hnRNP proteins.
    Deanna Green, Christie Johnson, Henry Hagan, Maja Kodani, Anita Corbett
    Department of Biochemistry, Emory University, 1510 Clifton Rd., NE, Atlanta, GA 30322, USA (acorbe2@emory.edu)

  • 5-14
    •   Dissection of the karyopherin alpha NLS binding pockets: Identification of in vivo NLS cargoes.
    Sara W. Leung, J.S. Holmes, M.T. Harreman, A.E. Hodel, Anita H. Corbett
    Biochemistry, Emory University, 1510 Clifton Rd, Atlanta, GA 30322, USA (sleung@emory.edu)

  • 5-15
    •   Subnuclear localization of tRNA genes in Saccharomyces cerevisiae.
    Martin Thompson, Paul Good, Li Wang, David Engelke
    Biological Chemistry, University of Michigan, 1150 W. Medical Ctr, Ann Arbor, MI 48109-0606, USA (proteios@umich.edu)

  • 5-16
    •   Suppressors of tRNA gene mediated silencing suggest an intimate link between the tRNA and rRNA biosynthetic pathways.
    Paul Good, Li Wang, Martin Thompson, David Engelke
    Department of Biological Chem., University of Michigan, 1301 E. Catherine St, Ann Arbor, MI 48109-0606, USA (paulgood@umich.edu)

  • 5-17
    •   Quality control of the mRNA and the integration of nuclear activities.
    Domenico Libri (1), Jocelyne Boulay (1), Rune Thomsen (2), Torben Heick Jensen (2)
    (1) CGM, CNRS, av de la Terrasse, Gif-sur-Yvette, 91190, France (libri@cgm.cnrs-gif.fr); (2) Department of Molecular Biology, Aarhus University, 8000 Aarhus C., Denmark

  • 5-18
    •   Getting to the heart of tRNA export.
    Rebecca Hurto (1), Amy Tong (2), Charlie Boone (2), Anita K Hopper (1)
    (1) Biochemistry and Molecular Biol., Pennsylvania State Coll. of Medicine, 500 University Drive, Hershey, PA 17078, USA (rlh223@psu.edu); (2) Banting & Best Dept. of Medical Research, Univerity of Toronto, 112 College Street, Toronto, Ontario, Canada M5G 1L6

  • 5-19
    •   Structural and dynamic functions establish chromatin domains.
    Kojiro Ishii (1), Clayton Lin (2), Ghislaine Arib (2), Ulrich K. Laemmli (2)
    (1) Division of Cell Biology, Institute of Life Science, Kurume University, 2432-3 Aikawa-machi, Kurume, Fukuoka 839-0861, Japan; (2) Molecular Biology, University of Geneva, 30 Quai Ansermet, Geneva, 1211, Switzerland (clayton.lin@molbio.unige.ch)

    Poster Session 6 - Chromosomes: replication, recombination, repair

  • 6-1
    •   The checkpoint protein Rad24p of Saccharomyces cerevisiae is involved in processing Double-Strand Break ends, recombination partner choice and recovery after repair.
    Yael Aylon, Martin Kupiec
    Mol. Micro. and Biotech., Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel (ayalo@post.tau.ac.il)

  • 6-2
    •   ELG1, a RFC-related gene, is involved in telomere length regulation.
    Sarit Smolikov, Anat Krauskopf
    Molecular Microbiology, Tel-Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel (saritsmol@hotmail.com)

  • 6-3
    •   Novel genes involved in telomere length regulation in Saccharomyces cerevisiae.
    Tal Yehuda, Anat Krauskopf
    Molecular Microbiology, Tel-Aviv University, Ramat Aviv, Tel-Aviv, 69978, Israel (talye@tau.post.ac.il)

  • 6-4
    •   Reversible inactivation of TRP5, CYH2 and MET13 dominate alleles in heterozygous yeast cells.
    Maxim Ivanov, Anna Aksenova, Ludmila Mironova
    Department of Genetics, St. Petersburg State University, Universitetskaya nab, Saint-Petersburg, 199034, Russia (aranarh@btc.bio.pu.ru)

  • 6-5
    •   A look at yeast telomeres.
    Lubomir Tomaska (1), Smaranda Willcox (2), Judita Sadovska (1), Jozef Nosek (3), Jack D. Griffith (2)
    (1) Department of Genetics, Comenius University, Faculty of Natural Sciences, Mlynska dolina B-1, 842 15 Bratislava, Slovakia (sadovska@fns.uniba.sk); (2) Lineberger Comprehensive Cancer Center and Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599, USA; (3) Department of Biochemistry, Comenius University, Faculty of Natural Sciences, Mlynska dolina CH-1, 842 15 Bratislava, Slovakia

  • 6-6
    •   The contribution of Pol Zeta, Rev1p and Pol Eta in UV induced mutagenesis in Saccharomyces cerevisiae.
    Ana I. Belo (1), Tineke de Ruijter (1), Hans den Dulk (1), Marcel Tijsterman (1), Leon Mullenders (2), Jaap Brouwer (1)
    (1) Molecular Genetics, Leiden Institute of Chemistry, Einsteinweg 55, Leiden, 2333 CC, The Netherlands (a.i.van.wijk@chem.leidenuniv.nl); (2) Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands

  • 6-7
    •   Genome-wide screen for genes controlling sensitivity to mutagenic base analogs in yeast.
    Elena Stepchenkova (1), Vladimir Alenin (1), Youri Pavlov (2)
    (1) Department of Genetics, St-Petersburg State University, Universitetskaja 7/9, St-Petersburg, 199034, Russia (stepchenkova@yahoo.com); (2) Laboratory of Molecular Genetics, NIEHS, NIH, P.O. Box 12233, Research Triangle Park NC, 27709, USA

  • 6-8
    •   Nuclear organization of DNA double-strand break repair.
    Michael Lisby (1), Jacqueline Barlow (1), Uffe Mortensen (2), Rodney Rothstein (1)
    (1) Department of Genetics & Development, Columbia University, College of Physicians & Surgeons, 701 West 168th Street, New York, NY 10032-2704, USA (ml676@columbia.edu); (2) Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Bldg. 223, DK-2800 Lyngby, Denmark

  • 6-9
    •   Segmental duplications in Saccharomyces cerevisiae.
    Joseph Schacherer, Jacky de Montigny, Jean-Luc Souciet, Serge Potier
    Groupe levure, Lab.de Microbio. et Génétique, rue Goëthe, Strasbourg, 67083, France (schacherer@gem.u-strasbg.fr)

  • 6-10
    •   Repair of UV induced DNA-damage in centromeres of S. cerevisiae.
    Christoph Capiaghi, Fritz Thoma
    Departement Biologie, Institut für Zellbiologie, ETH Hönggerberg, Zürich, 8093, Switzerland (christoph.capiaghi@cell.biol.ethz.ch)

  • 6-11
    •   The Csm1 protein of Saccharomyces cerevisiae is responsible for correct chromosome segregation during meiosis.
    Monika Wysocka, Anna Kurlandzka
    Department Genetics, IBB, PAS, Pawinskiego 5A, Warszawa, 02-106, Poland (monikaw@poczta.ibb.waw.pl)

  • 6-12
    •   The RAD9 and RAD24 signalling pathway is required for induction of RAD54 transcription in response to DNA damage by oxidising agents but not by alkylating agents.
    Salah Abu-Jadallah, Richard Walmsley
    Biomolecular Sciences, UMIST, Sackville Street, Manchester, M60 1QD, UK (s.abu-jadallah@student.umist.ac.uk)

  • 6-13
    •   Mapping of DNA double-strand break points at the meiotic ura4A hotspot of recombination in Schizosaccharomyces pombe.
    Benjamin Sakem, Micheal Baur, Edgar Hartsuiker, Elizabeth Lehman, Katja Ludin, Jürg Kohli
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, Bern, 3012, Switzerland (benjamin.sakem@izb.unibe.ch)

  • 6-14
    •   ELG1, a novel yeast gene required for genome stability, forms a complex related to Replication Factor C.
    Shay Ben-Aroya, Amnon Koren, Batia Liefshitz, Rivka Steinlauf, Martin Kupiec
    Mol Micro&Biotech, Tel-Aviv university, Ramat-Aviv, Tel-Aviv, 69978, Israel (shayben@post.tau.ac.il)

  • 6-15
    •   Functional characterization of the Ctf19 central kinetochore complex of budding yeast.
    Isabelle Pot (1), Nicolas Szapiel (2), Amy Tong (3), Victoria Aneliunas (1), Michael Snyder (4), Charlie Boone (3), Jackie Vogel (2), Philip Hieter (1)
    (1) Dpt. of Biochemistry, Univ. of British Columbia, 980 W. 28th Avenue, Vancouver, BC V5Z 4H4, Canada (pot@cmmt.ubc.ca); (2) Dpt. of Biology, McGill Univ., 1205 Dr. Penfield Ave, Montreal, QC H3A 1B1, Canada; (3) Banting & Best Dpt. of Medical Research, Univ. of Toronto, 112 College Street, Toronto, ON M5G 1L6, Canada; (4) Dpt. of Molecular, Cellular and Developmental Biology, Yale Univ., 266 Whitney Ave, New Haven, CT 06520, USA

  • 6-16
    •   Distinct mutants define separate functions of the Spc24 kinetochore protein.
    Vivien Measday (1), Irene Barrett (1), Kim Andrews (2), Ben Monpetit (1), Brenda Andrews (2), Phil Hieter (1)
    (1) Medical Genetics, CMMT, University of British Columbia, 950 West 28th Avenue, Vancouver, B.C., V5Z 4H4, Canada (vmeasday@cmmt.ubc.ca); (2) Dept. of Medical Genetics and Microbiology, University of Toronto, Rm. 4285 Medical Sciences Building, 1 Kings College Circle, Toronto ON M5S 1A8, Canada

  • 6-17
    •   Position, formation and stability of a site and strand specific DNA break at the mat1 locu in fission yeast.
    Atanas Kaykov, Allyson Holmes, Benoit Arcangioli
    Jacques Monod, Pasteur Institue, 25 rue du Dr Roux, Paris, 75724, France (barcan@pasteur.fr)

  • 6-18
    •   Isolation and Characterization of novel genes required for DNA double strand breaks in Schizosaccharomyces pombe.
    Hideo Shinagawa (1), Takashi Morishita (1), Yasuhiro Tsutsui (1), Shiho Horiuchi (1), Hirofumi Morikawa (1), Fumiko Furukawa (1), Hiroshi Iwasaki (2)
    (1) Res. Inst. Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, 565-0871, Japan (shinagaw@biken.osaka-u.ac.jp); (2) Graduate School of Integrative Science, Yokohama City University, Tsurumi, Yokohama 230-0045, Japan

  • 6-19
    •   The silent information regulators SIR2-4 negatively regulate chromosomal DNA replication.
    Donald Pappas, Michael Weinreich
    Chromosome Replication Lab, Van Andel Research Institute, 333 Bostwick Ave. NE, Grand Rapids, MI 49503, USA (don.pappas@vai.org)

  • 6-20
    •   Equal sister-chromatid exchange is a major mechanism of double-strand-break repair in yeast.
    Felipe Cortés-Ledesma, Sergio González-Barrera, Ralf E. Wellinger, Andrés Aguilera
    Dpto. Genética, Fac. Biología, Universidad de Sevilla, Reina Mercedes 6, Sevilla, 41012, Spain (fcortes@us.es)

  • 6-21
    •   Impairment of chromatin assembly confers hyperrecombination.
    Felix Prado, Andres Aguilera
    Genetica, Uni. Sevilla, Reina Mercedes, 6, Sevilla, 41012, Spain (fprado@us.es)

  • 6-22
    •   Does the ongoing DNA replication trigger checkpoint responses?
    Maria M. Magiera, Alain Devault, Etienne Schwob
    IGM, CNRS, 1919 route de Mende, Montpellier, 34293, France (magiera@igm.cnrs-mop.fr)

  • 6-23
    •   Searching for chromosome fragile sites in budding yeast sic1 mutants.
    Audrey Ceschia, Léon Dirick, Etienne Schwob
    Inst. Molecular Genetics, CNRS, 1919 rte de Mende, Montpellier, 34293, France (schwob@igm.cnrs-mop.fr)

  • 6-24
    •   Genetic and structural characterization of spontaneous karyotype rearrangements in a natural yeast strain.
    Benjamin Piña (1), Enric Bartra (2), David Carro (1), Jose Garcia-Martinez (3), Jose Enrique Perez-Ortin (3)
    (1) Molecular and Cellular Biology, IBMB-CSIC, Jordi Girona, 18, Barcelona, 08034, Spain (bpcbmc@cid.csic.es); (2) Institut Català de la Vinya i el Vi. Amàlia Soler 29, 08720 Vilafranca del Penedès, Barcelona, Spain; (3) Departamento de Bioquímica y Biología Molecular. Facultad de Biológicas, Universitat de València. Dr. Moliner 50. 46100 Burjassot, Valencia, Spain.

  • 6-25
    •   Contractions and Expansions of CAG/CTG Trinucleotide Repeats occur during Ectopic Gene Conversion in Yeast, by a MUS81-independent Mechanism.
    Guy-Franck Richard, Bernard Dujon
    Structure/ Dynamics of Genomes, Institut Pasteur, 25 rue du Dr Roux, Paris, 75015, France (gfrichar@pasteur.fr)

  • 6-26
    •   Genome instability and population dynamics in natural wine yeasts.
    Manuel Ramírez (1), Jesés Ambrona (1), Antonia Vinagre (1), Felipe Molina (2), José E. Rebollo (2)
    (1) Microbiología (Ant Rectorado), Universidad de Extremadura, Avda. de Elvas s/n, Badajoz, 06071, Spain (mramirez@unex.es); (2) Departamento de Genética. Facultad de Ciencias. Universidad de Extremadura. 06071 Badajoz. Spain.

  • 6-27
    •   Experimental evidence for spontaneous formation of large segmental duplications in the yeast genome.
    Romain Koszul, Bernard Dujon, Gilles Fischer
    Gen. Mol. Des Levures, Institut Pasteur, 25, rue du Dr Roux, Paris cedex 15, 75724, France (koszul@pasteur.fr)

  • 6-28
    •   The transcriptional response to DNA damage in Saccharomyces cerevisiae.
    Lindsey Walsh (1), Andrew Hayes (1), Stephen Oliver (1), Richard Walmsley (2)
    (1) School of Biological Sciences, University of Manchester, Oxford Rd, Manchester, M13 9PT, UK (lindsey.walsh@gentronix.co.uk); (2) Gentronix Ltd, The Fairbairn Building, Sackville St, Manchester, M60 1QD, UK

  • 6-29
    •   Nuclear architecture and double strand breaks repair in yeast subtelomeres.
    Emmanuelle Fabre, Cécile Fairhed, Bernard Dujon
    Structure/Dynamics of Genomes, Institut Pasteur, 25 rue du Dr. Roux, Paris, 75015, France (efabre@pasteur.fr)

  • 6-30
    •   DNA polymerase epsilon — the leading strand DNA polymerase?
    Olga Chilkova, Nasim Sabouri, Isabelle Isoz, Erik Johansson
    Dept of Medical Biochemistry, Umeå University, Umeå University, Umeä, SE-901 87, Sweden (erik.johansson@medchem.umu.se)

  • 6-31
    •   Sequences required for chromosomal replicator function in Kluyveromyces lactis.
    Carmela Irene (1), Clelia Maciariello (1), Francesco Cioci (2), Giorgio Camilloni (2), Carol S. Newlon (3), Lucia Fabiani (1)
    (1) Dipartimento Biologia Cellulare e Sviluppo, Università La Sapienza, P.le A. Moro, 5, 00185 Roma, Italy (carmelairene@hotmail.com); (2) Dipartimento Genetica e Biologia Molecolare, Università La Sapienza, P.le A. Moro, 5, 00185 Roma, Italy; (3) Dept. Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, NJ 07103 USA

  • 6-32
    •   Selection and molecular studies of chromosomal rearrangements in diploid Saccharomyces cerevisiae strains.
    Yves Tourrette, Joseph Schacherer, Jacky de Montigny, Serge Potier, Jean-Luc Souciet
    Lab. de Microbio & Génétique, Université Louis Pasteur, 28, rue Goethe, Strasbourg, 67000, France (tourrette@gem.u-strasbg.fr)

  • 6-33
    •   A specific role for the evolutionarily conserved S. cerevisiae Spt4p in specialized chromatin at kinetochores and silenced heterochromatin regions.
    Munira Basrai, Luciana Espinoza
    Genetics, National Cancer Institute, 8901 Wisconsin Avenu, Bethesda, MD 20889, USA (basraim@nih.gov)

  • 6-34
    •   Genomic dissection of the yeast kinetochore: a three pronged approach.
    Kristin Baetz (1), Vivien Measday (1), Julie Guzzo (2), Karen Yuen (1), Phil Hieter (1), Brenda Andrews (2)
    (1) CMMT-Dept. of Medical Genetics, University of British Columbia, 980 West 28th Ave, Vancouver, BC V5Z 4H4, Canada (kbaetz@cmmt.ubc.ca); (2) Dept. of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Toronto ON, M5S 1A8, Canada

  • 6-35
    •   Cyclin B-Cdk activity stimulates meiotic re-replication in budding yeast.
    Randy Strich (1), Michael Mallory (1), Katrina Cooper (2)
    (1) Cell and Developmental Biology, Fox Chase Cancer Center, 7701 Burholme Ave, Philadelphia, PA 19111, USA (r_strich@fccc.edu); (2) Drexel University College of Medicine, 245 N. 15th St. Philadelphia, PA 19102, USA

  • 6-36
    •   Characterization of a Trf4/Pol sigma complex from S. cerevisiae.
    Dena R. Carson, Carrie Adams, Jonathan P. Carson, Anupriya Dutta, Michael F. Christman
    Genetics and Genomics, Boston University, 715 Albany St. E-604, Boston, MA 02118, USA (drcarson@bu.edu)

  • 6-37
    •   Microarray-based analysis of DNA replication dynamics in a replication-defective mutant.
    Heather J. McCune, M.K. Raghuraman, Walton L. Fangman, Bonita J. Brewer
    Department of Genome Sciences, University of Washington, Box 357730, Seattle, WA 98195, USA (heather@genetics.washington.edu)

  • 6-38
    •   Identification of the fission yeast nbs1 + gene involved in DNA double-strand break repair.
    Tomofumi Nakazaki (1), Yufuko Akamatsu (1), Hideo Shinagawa (2), Masaru Ueno (3), Hiroshi Iwasaki (1)
    (1) Intergrated Science, Yokohama City University, Tsurumi, Yokohama, 230-0045, Japan (iwasaki@tsurumi.yokohama-cu.ac.jp); (2) Research Institute for microbial Diseases, Osaka University, Suita Osaka, 565-0871 Japan; (3) Department of Chemistry, Faculty of Science, Shizuoka University 836 OYA, Shizuoka 422-8529, Japan

  • 6-39
    •   Requirement of HSM3 for spontaneous mutagenesis in Saccharomyces cerevisiae
    Vladimir Korolev, Irina Fedorova, Svetlana Kovaltzova, Ludmila Gracheva, Tatiana Evstuhina
    Laboratory of Eucaryote Genetics, Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, RAS, 188350 Orlova Roscha, Gatchina, Leningrad distr., Russia (lge@omrb.pnpi.spb.ru)

  • 6-40
    •   The importance of Sgs1p and the MRX-complex in regulating the terminal DNA at telomeres.
    Raymund Wellinger, Catherine LeBel, Michel Larrivée
    Microbiology, Université de Sherbrooke, 3001 12 Ave N, Sherbrooke, J1H 5N4, Canada (Raimund.Wellinger@USherbrooke.ca)

  • 6-41
    •   Elg1 forms a novel RFC-like complex that is important for replication fork integrity.
    Mohammed Bellaoui (1), Michael Chang (1), Jiongwen Ou (1), Hong Xu (2), Charles Boone (3), Grant W. Brown (1)
    (1) Department of Biochemistry, University of Toronto, 1 King's College Cir, Toronto, ON M5S 1A8, Canada (grant.brown@utoronto.ca); (2) Department of Medical Genetics and Microbiology, University of Toronto, 112 College Street, Rm 411, Toronto, ON, Canada, M5G 1L6; (3) Department of Medical Genetics and Microbiology, 112 College Street, Rm 411, Toronto, ON, Canada, M5G 1L6

  • 6-42
    •   Proteins necessary for the structural integrity of the genome: their role in DNA repair and recombination.
    Lena Ström, Hanna Betts Lindroos, Camilla Sjögren
    Cell and Molecular Biology, Karolinska Institutet, Berzelius väg 35, Stockholm, 171 77, Sweden (lena.strom@cmb.ki.se)

  • 6-43
    •   Genetic interactions of mph1 mutants from Saccharomyces cerevisiae imploy an involvement in error free bypass of DNA lesions.
    K. Anke Schürer, Christian Rudolph, Wilfried Kramer
    Inst. f. Mikrobiologie/Genetik, Universität Göttingen, Grisebachstr. 8, Göttingen, D-37077, Germany (aschuer@gwdg.de)

  • 6-44
    •   Involvement of RSC nucleosome-remodeling complex of Saccaromyces cerevisiae in DNA repair.
    Hirofumi Koyama, Ken-ichi Nakamura, Eiko Tsuchiya
    Molecular Biotechnology, Hiroshima University, 1-3-1, Kagamiyama, Higashi-Hiroshima, 739-8530, Japan (etsuchi@hiroshima-u.ac.jp)

  • 6-45
    •   Transcription-dependent recombination in yeast rDNA.
    Yasushi Takeuchi, Takashi Horiuchi, Takehiko Kobayashi
    Lab. of Gene Exp. and Reg., Natl. Inst. for Basic Biology, Myodaiji-cho, Okazaki, 444-8585, Japan (koba@nibb.ac.jp)

  • 6-46
    •   The Rad53-mediated checkpoint response is required to stabilize replisome-fork association when DNA replication is pausing.
    Chiara Lucca (1), Fabio Vanoli (1), Cecilia Cotta-Ramusino (1), Hiroyuki Araki (2), Marco Foiani (1)
    (1) IFOM, FIRC, Via Adamello 16, Milano, 20139, Italy (lucca@ifom-firc.it); (2) National Institute of Genetics, Yata 1111, Mishima, Shizuoka, Japan.

  • 6-47
    •   Targeted Stimulation of Meiotic Recombination in S. cerevisiae.
    Valérie Borde (1), Christine Mézard (1), Hajime Murakami (2), Ana Pecina (1), Kathleen Smith (1), Norio Uematsu (1), Michèle Vedel (1), Kunihiro Ohta (2), Alain Nicolas (1)
    (1) UMR 144 CNRS-IC, Institut Curie-Sect Recherche, 26 rue d'Ulm, Paris Cedex 05, 75248, France (alain.nicolas@curie.fr); (2) The Graduate School of Science and Engineering, Saitama University, Urawa-shi, Saitama 338-8570, Japan

  • 6-48
    •   A dissection of the molecular mechanisms behind DNA double strand break repair.
    Tanja Thybo (1), Michael Lisby (2), Rodney Rothstein (2), Uffe Mortensen (1)
    (1) BioCentrum-DTU, Technical Univ. of Denmark, Building 223, Lyngby, 2800, Denmark (ttf@biocentrum.dtu.dk); (2) Department of Genetics and Development, Columbia University, USA

  • 6-49
    •   Localization of recombination intermediates in S. cerevisiae using GFP-based variants in vivo.
    Christian Müller (1), Michael Lisby (2), Rodney Rothstein (2), Uffe H. Mortensen (1)
    (1) Center of Process Biotechnology, Biocentrum-DTU, Søltofts Plads, Building 223, Technical University of Denmark (cm@biocentrum.dtu.dk); (2) Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, New York 10032-2704, USA.

  • 6-50
    •   Characterisation of the checkpoint and repair functions of two Saccharomyces cerevisiae FHA/RING finger containing proteins.
    Serge Gravel, Stephen Jackson
    Wellcome/CRUK Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom (sg339@cam.ac.uk)

  • 6-51
    •   Analysis of replication fork movement in mutants defective in RNA polymerase II transcription elongation.
    Ralf E. Wellinger, Andrés Aguilera
    Dpto de Genetica, Universidad de Sevilla, Avd Reina Mercedes 6, Sevilla, 41012, Spain (wellinger@us.es)

  • 6-52
    •   Investigation of Rad52 self-association and Rad52 nuclear transport in Saccharomyces cerevisiae.
    Iben Plate (1), Michael Lisby (2), Rodney Rothstein (2), Uffe H. Mortensen (1)
    (1) BioCentrum, DTU, Technical Univ. of Denmark, Building 223, Kgs. Lyngby, 2800, Denmark (ipl@biocentrum.dtu.dk); (2) Department of Genetics and Development, Columbia University, USA.

  • 6-53
    •   Characterization of the SMC5/SMC6 complex involved in chromosome maintenance and stability.
    Hanna Betts Lindroos, Lena Ström, Camilla Sjögren
    Cellular and molecular biology, Cellular and molecular biology, Berzelius v. 35, Stockholm, 171 77, Sweden (hanna.betts.lindroos@cmb.ki.se)

  • 6-54
    •   Effect of prion [ PSI + ] on specific starvation-associated mutagenesis in Saccharomyces cerevisiae.
    Tomás Cápal, Vladimír Vondrejs
    Dpt. of Genetics and Microbiol, Charles University, Vinicná 5, Prague 2, 12843, Czech Republic (ctomas@emai.cz)

  • 6-55
    •   Replication of UV-damaged templates can activate a Tel1/MRX-dependent checkpoint response.
    Veronica Baldo, Michela Clerici, Giovanna Lucchini, Maria Pia Longhese
    Biotecnologie e Bioscienze, Università di Milano-Bicocca, P.za della Scienza,2, Milano, 20126, Italy (veronica.baldo@unimib.it)

  • 6-56
    •   A high molecular weight protein complex containing Srs2, Sgs1 and Mre11 repair factors is reorganised in response to DNA damage and checkpoint activation.
    Irene Chiolo (1), Walter Carotenuto (1), Giulio Maffioletti (1), Michele Giannattasio (2), Marco Muzi-Falconi (2), John H. Petrini (3), Marco Foiani (1), Giordano Liberi (1)
    (1) IFOM, FIRC, Via Adamello 16, Milano, 20139, Italy (chiolo@ifom-firc.it); (2) DGBM, Universita' degli Studi di Milano, Milan, Italy; (3) University of Wisconsin Medical School, New York, USA

  • 6-57
    •   Different impacts of nonhomologous end-joining on gene-targeting in Saccharomyces and Klyveromyces.
    Andreas Kegel, Göran Bylund, Jimmy Sjöstrand, Stefan Äström
    Wenner-Gren Institute, Stockholm University, Svante Arrheniusv 18, Stockholm, 10691, Sweden (andreas.kegel@devbio.su.se)

  • 6-58
    •   Mre11 nuclease activity is required for the repair of topoisomerase II mediated DNA damage.
    John Nitiss, Alexandre Stepanov, Karin Nitiss
    Molecular Pharmacology, St. Jude Children's Res. Hosp., 332 N. Lauderdale, Memphis, TN 38015, USA (john.nitiss@stjude.org)

  • 6-60
    •   Mechanism of telomerase recruitment at a double-strand break site.
    Alessandro Bianchi, Simona Negrini, Isabelle Howald, David Shore
    Dept. of Molecular Biology, University of Geneva, 30 quai Ansermet, Geneva, CH-1211 G4, Switzerland (Alessandro.Bianchi@molbio.unige.ch)

  • 6-61
    •   Survival and adaptation to DNA damage mediated by DNA topoisomerases in S. pombe : Checkpoints and the roles of non-homologous end-joining pathway components.
    Mobeen Malik, John L. Nitiss
    Dept. Molecular Pharmacology, St Jude Children Research Hosp, 322 N. Lauderdale, Memphis, TN 38105, USA (Noor-E-Mobeen.Malik@stjude.org)

  • 6-62
    •   DNA double-strand break repair pathways regulate a morphogenetic switch in yeast.
    Adrian Halme, Cora Styles, Kexin Yu, Gerald Fink
    Whitehead Institute, M.I.T., 9 Cambridge Center, Cambridge, MA 02142, USA (halme@wi.mit.edu)

  • 6-63
    •   Characterization of Sph2p, a fission yeast member of SP-RING family of SUMO-Ligases.
    Blerta Xhemalce (1), Genevieve Thon (2), Benoit Arcangioli (1)
    (1) Struct. and Dynamic of Genomes, Pasteur Institute, rue du Dr.Roux, Paris, 75015, France (blerta@pasteur.fr); (2) Department of Genetics, Institute of Molecular Biology, University of Copenhagen, OEster Farimagsgade 2A DK-1353 Copenhagen K Denmark.

  • 6-64
    •   Roles of DNA topoisomerases in cell survival following exposure to DNA damage.
    Karin Nitiss, Alexandre Stepanov, John Nitiss
    Molecular Pharmacology, St. Jude Children's Res. Hosp., 332 N. Lauderdale, Memphis, TN 38105, USA (karin.sykes@stjude.org)

  • 6-65
    •   Analysis of transcription termination sequences and transcription levels in relation to ARS localization and efficiency on Chromosome III in Saccharomyces cerevisiae.
    Yves Sucaet, Christi Magrath
    Biology/Environmental Science, Troy State University, Math/Science Complex, Troy, AL 36082, USA (yves.sucaet@usa.net)

  • 6-66
    •   Carbon-source dependent changes in spontaneous mutation rate are not a function of generation time.
    Michael Hamilton, Nicholas Fairbridge, R. C. von Borstel
    Dept of Biological Sciences, University of Alberta, BSB, Edmonton, Alberta, T6G 2E9, Canada (rc.von-borstel@ualberta.ca)

  • 6-67
    •   The DNA damage checkpoint and PKA pathways converge on APC substrates and Cdc20 to regulate mitotic progression.
    Jennifer S. Searle (1), Kaila L. Schollaert (1), Benjamin J. Wilkins (2), Yolanda Sanchez (1)
    (1) Molecular Genetics, Biochem, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267-0524, USA (searlej@email.uc.edu); (2) Division of Molecular Cardiovascular Biology, Department of Pediatrics, Children's Hospital Medical Center Cincinnati, OH 45267-0524, USA

  • 6-68
    •   Functional analysis of the fission yeast swi5 + gene, which is involved in mating-type switching and recombination repair.
    Yufuko Akamatsu (1), Dorota Dziadkowiec (2), Hideo Shinagawa (3), Hiroshi Iwasaki (1)
    (1) Grad. Sch. of Integrated Sci., Yokohama City University, 1-7-29, Suehiro-cho, Tsurumi, Yokohama, 230-0045, Japan (yufuko@tsurumi.yokohama-cu.ac.jp); (2) Institute of Microbiology, Wroclaw University, Przybyszewskiego 63,51-148, Wroclaw, Poland; (3) Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan

  • 6-69
    •   UBC9 functions to protect cells from DNA topoisomerase I poisons.
    Herve R. Jacquiau (1), Robert C. A. M. van Waardenburg (1), Robert J. D. Reid (2), Mary-Ann Bjornsti (1)
    (1) Department of Molecular Pharmacology, St. Jude Children's Research Hospital, 332 N. Lauderdale St., Memphis, TN 38105 USA (herve.jacquiau@stjude.org); (2) Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107 USA

  • 6-70
    •   Alteration in TAH11 function adversely affect cell viability in response to replication-induced DNA damage.
    Nabil Matmati, Hong Guo, Mary-Ann Bjornsti
    Molecular Pharmacology, St. Jude Children's Res. Hosp., 332 N. Lauderdale, Memphis, TN 38105, USA (nabil.matmati@stjude.org)

  • 6-71
    •   The transcriptional regulator and mRNA deadenylase Ccr4 is a novel component of the DNA damage response.
    Robert N. Woolstencroft, Mike Tyers, Daniel Durocher
    Samuel Lunenfeld Research Inst, Mount Sinai Hospital, 600 University Ave, Toronto, M5G 1X5, Canada (rob@mshri.on.ca)

  • 6-72
    •   The new link between proteasome 20S and post-replication DNA repair pathway in Saccharomyces cerevisiae.
    Adrianna Skoneczna, Agnieszka Podlaska, Justyna McIntyre, Ewa Sledziewska-Gojska
    Mutagenesis & DNA Repair Lab, IBB PAS, Pawinskiego 5a, Warszawa, 02-106, Poland (ada@ibb.waw.pl)

  • 6-73
    •   Telomere binding and regulation by RAP1 homologs.
    Johan Wahlin, Monika Rosén, Marita Cohn
    Cell and Organism Biology, Lund University, Solvegatan 35, Lund, S-22362, Sweden (marita.cohn@cob.lu.se)

  • 6-74
    •   Regulation of the G1/S transition in fission yeast.
    Esben Are Nilsen, Marianne Synnes, Tonje Tvegård, Heidi Vebø, Alfredo Diez, Beata Grallert, Erik Boye
    Cell Biology, Cancer research, The Norwegian Radium Hospital, Montebello, Oslo, N-0310, Norway (alfredo.diez@klinmed.uio.no)

  • 6-75
    •   Involvement of RVB1 and RVB2 in DNA double-strand break repair and UV damage response in Saccharomyces cerevisiae.
    Slobodanka Radovic, Viviana Rapisarda, Carlo V. Bruschi
    Microbiology, ICGEB, AREA Science Park, Trieste, I-34012, Italy (radovic@icgeb.org)

  • 6-76
    •   Hyper-recombination and hyper-resistance to DNA damage in fission yeast defective in ubiquitin-conjugating enzyme Ubc7.
    Fumiaki Yamao (1), Joon-Hyun Park (1), Hiroshi Iwasaki (2), Yufuko Akamatsu (2)
    (1) Molecular Genetics, Natl. Inst. Genetics, 1111 Yata, Mishima, 411-8540, Japan (fyamao@lab.nig.ac.jp); (2) Graduate School of Integrated Science, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.

  • 6-77
    •   Aphidicolin Sensitivity of Yeast and Neurospora DNA polymerases.
    Nawin Mishra (1)
    (1) Dept. Biological Sciences, University of South Carolina, Columbia, SC, USA (mishra@biol.sc.edu)

    Poster Session 7 - Transcription: RNA polymerase, chromatin, factors

  • 7-1
    •   Localication of cis-acting regulatory elements in promoter region of SUP35 gene of Saccharomyces cerevisiae and phenotypic analysis of mutants with their disruptions.
    Natalia A. Ryabinkova, Andrey S. Borchsenius, Sergey G. Inge-Vechtomov
    Genetic and Breeding, St. Petersburg State Universit, Universytetskaya 7/9, St. Petersburg, 199034, Russian Federation (nriabinkova@yahoo.com)

  • 7-2
    •   Characterisation of the Ssn6-Tup1 corepressor in Schizosaccharomyces pombe.
    Fredrik Fagerström-Billai, Anthony Wright
    Natural Science Department, Sodertorn University / KI, Alfred Nobel Alle 3, Huddinge, 14152, Sweden (fredrik.fagerstrom-billai@cbt.ki.se)

  • 7-3
    •   Structural and functional interactions between RNA polymerase II and its elongation factors.
    Elena Shematorova (1), Pierre Thuriaux (1), Maxime Wéry (2), Benoît Van Driessche (2), Jean Vandenhaute (2), Vincent Van Mullem (2)
    (1) Département de SBGM, CEA/Saclay, Bât 144, Gif-sur-Yvette, 91191, France (elenashe@matthieu.saclay.cea.fr); (2) Laboratoire de Génétique Moléculaire (URBM), Facultés Universitaires Notre-Dame de la Paix. 61, rue de Bruxelles, B-5000 Namur (Belgique).

  • 7-4
    •   Dicer is required for chromosome segregation and gene silencing in fission yeast cells.
    Ingela Djupedal (1), Patrick Provost (2), Rebecca Silverstein (1), David Dishart (2), Julian Walfridsson (1), Barbara Kniola (1), Bengt Samuelsson Olof Rådmark (2), Karl Ekwall (1)
    (1) Department of Natural Sciences, University College Sodertorn, Alfred Nobels Allé 3, Huddinge, 14189, Sweden (ingela.djupedal@sh.se); (2) Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institute, Stockholm, S-17177, Sweden

  • 7-5
    •   Modulation of transcription factor function by an amino acid: activation of Put3p by proline.
    Christopher A. Sellick, Richard J. Reece
    School of Biological Sciences, The University of Manchester, Oxford Road, Manchester, M13 9PT, UK (christopher.a.sellick@stud.man.ac.uk)

  • 7-6
    •   Complementation analysis and nuclear targeting of Saccharomyces cerevisiae FUN20/PRP45, the homolog of the coactivator SKIP.
    Katerina Martinkova, Michal Skruzny, Pavel Lebduska, Iva Fukova, Petr Folk, Frantisek Puta
    Physiol. Dev. Biology, Charles University, Vinicna 7, Prague, 12800, Czech Republic (k_martinkova@hotmail.com)

  • 7-7
    •   Connecting transcription to chromatin and mRNA processing.
    Stephen Buratowski
    Biol. Chemistry and Mol. Pharm, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA (steveb@hms.harvard.edu)

  • 7-8
    •   Gcn4 participates in the nitrogen discrimination pathway in Saccharomyces cerevisiae.
    Alicia Gonzalez (1), Lina Riego (1), Eduardo Sosa (1), Cristina Aranda (1), Lourdes Valenzuela (1), Alexander DeLuna (1), Jose M. Cantu (2)
    (1) Molecular Genetics, National University of Mexico, Av Universidad S/N, Mexico City, 04510, Mexico (amanjarr@ifisiol.unam.mx); (2) Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, 44034 Guadalajara, Jalisco, Mexico

  • 7-9
    •   Characterization of jumonji-domain interacting proteins in yeast.
    Susanna Tronnersjö (1), Darius Balciunas (2), Guo-Zhen Hu (3), Christine Hanefalk (3), Hans Ronne (3)
    (1) Department of Plant Biology and Forrest Genetics, Swedish University of Agricultural Sciences, Box 7080, 750 07 Uppsala, Sweden (Susanna.Tronnersjo@vbsg.slu.se); (2) Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA; (3) Dep of Plantbiol & Forrestgen, SLU, Box 7080, Uppsala, 750 07, Sweden

  • 7-10
    •   Regulation of gene expression and chromatin structure.
    Claudia Wagner (1), Oliver Valerius (2), Gerhard H. Braus (2)
    (1) (cwagner2@gwdg.de); (2) Georg-August-University, Microbiology and Genetics, Grisebachstr. 8, Göttingen, 37077, Germany

  • 7-11
    •   Iron homeostasis in Saccharomyces cerevisiae : studies on the relationships between the transcription factors Aft1 and Aft2 by identification of target genes.
    Maïté Courel, Jean Michel Camadro, Pierre Louis Blaiseau
    Institut Jacques Monod, CNRS/ Universités Pari6/7, 2 place Jussieu, Paris, 75251, France (blaiseau@ijm.jussieu.fr)

  • 7-12
    •   Transcriptional coordination of metabolic pathways.
    Kenneth Dombek (1), Chris Tachibana (1), Trey Ideker (2), Nataly Kacherovsky (1), David Fox (1), Elton T. Young (1)
    (1) Dept of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195, USA (cxt@u.washington.edu); (2) Whitehead Institute, 9 Cambridge Center, Cambridge MA, USA

  • 7-13
    •   G2/M specific transcription - a tango between Ndd1 and Sin3 on and around the FHA domain of Fkh2.
    Helene Klug, Jiri Veis, Manfred Koranda, Gustav Ammerer
    Biochemistry & Mol. Cell. Biol, University of Vienna, Dr. Bohrgasse 9, Vienna, 1030, Austria (hel@abc.univie.ac.at)

  • 7-14
    •   Eukarya use a subset of all possible tRNA sequences to facilitate the recognition of their tRNA genes by the TFIIIC transcription factor.
    Christian Marck (1), Henri Grosjean (2)
    (1) Service de Biochimie et de Génétique Moléculaire, Bât 144, CEA/Saclay, 91191, Gif-sur-Yvette, France (christian.marck@cea.fr); (2) Laboratoire d'Enzymologie et de Biologie Structurale, Bât 34, CNRS, 91198, Gif-sur-Yvette, France

  • 7-15
    •   Domain characterization of Pdc2.
    Dominik Mojzita (1), Stefan Hohmann (2)
    (1) Department of Cell and Molecular Biology/Microbiology, Medicinaregatan 9C, S-405 30 Göteborg, Sweden (gmmdm@lundberg.gu.se); (2) CMB/Microbiology, Göteborg University, Medicinaregatan 9C, Göteborg, 40530, Sweden

  • 7-16
    •   Characterisation of HAT/HDAC interactions in fission yeast gene expression networks.
    Anna Johnsson (1,2), Yongtao Xue (1,2), Eliana Gómez (3), Susan Forsberg (3), Maria Lundin (1,2), Anthony Wright (1,2)
    (1) Södertörn University College, Natural Science Section, Alfred Nobels Allé 3, Huddinge, 141 89, Sweden (anna.johnsson@sh.se); (2) Department of Biosciences, Karolinska Institute, Novum, S-141 57 Huddinge, Sweden; (3) Molecular and Cell Biology Laboratory, The Salk Institute, La Jolla California 92037, USA

  • 7-17
    •   RSC loss of function results in genome transmission failure and gene repression relief.
    Colin Logie, Coen Campsteijn, Anil Ozdemir, Anne-Marie Collin
    Molecular Biology, Nijmegen University, PO Box 9101, Nijmegen, 6500-HB, The Netherlands (c.logie@ncmls.kun.nl)

  • 7-18
    •   Silencing of the cryptic mating type loci in Kluyveromyces lactis.
    Jimmy Sjöstrand, Andreas Kegel, Stefan Äström
    Developmental Biology, Wenner Greens Institute, Svante Arrheniusv 16, Stockholm 10691, Sweden (jimmy.sjostrand@devbio.su.se)

  • 7-19
    •   The mechanisms by which Gal3/KlGal1p activate Gal4p in response to galactose differ between S. cerevisae and K. lactis.
    Alexander Anders, Lutz Kapp, Karin D. Breunig
    Institute of Genetics, University of Halle, Weinbergweg 10, Halle, D-06099, Germany (breunig@genetik.uni-halle.de)

  • 7-20
    •   Ssn6 co-repressor and Nhp6A/B architectural factors are cooperatively responsible for Aft1-mediated transcriptional activation in Saccharomyces cerevisiae.
    George S. Fragiadakis (1), Dimitris Tzamarias (2), Despina Alexandraki (1)
    (1) FORTH/IMBB and Univ. of Crete, Biology, P.O. Box 1527, Heraklion, Crete, 71110, Greece (alexandr@imbb.forth.gr); (2) FORTH/Institute of Molecular Biology & Biotechnology and Hellenic Open University, School of Science and Technology

  • 7-21
    •   Structure-function comparison of nuclear RNA polymerases I and III of Schizosaccharomyces pombe and Saccharomyces cerevisiae.
    G.V. Shpakovski, E.K. Shematorova, G.M. Proshkina, N.A. Potapenko, T.V. Ovchinnikova, S.A. Proshkin
    Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia (gvs@mail.ibch.ru)

  • 7-22
    •   Regulation of ribosome biosynthesis.
    Jon Warner, Dipayan Rudra, Yu Zhao
    Cell Biology, Albert Einstein Col. Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA (warner@aecom.yu.edu)

  • 7-23
    •   Involvement of the pol II Mediator and 20 S catalytic subunit of the proteasome in regulating the yeast heat shock response.
    David S. Gross, Harpreet Singh, Donnie A. Davis, Geraldine Alba, Rachel R. Gross, Alexander M. Erkine
    Biochemistry & Mol. Biology, Louisiana State University HSC, 1501 Kings Highway, Shreveport, LA 71130-3932, USA (dgross@lsuhsc.edu)

  • 7-24
    •   Genome-wide localization of RNA polymerase III and its transcription factors : the RNA polymerase III genome.
    Olivier Harismendy (1), Gabrielle Gendrel-Lefeuvre (1), Christian Marck (1), Pascal Soularue (2), Xavier Gidrol (2), André Sentenac (1), Michel Werner (1), Olivier Lefebvre (1)
    (1) Service de Biochimie et de Génétique Moléculaire, bât 144, CEA-Saclay, 91191 Gif-sur-Yvette cedex, France (haris@matthieu.saclay.cea.fr); (2) Service de Génomique Fonctionnelle, CEA-Evry, 2 rue Gaston Crémieux CP22, 91057 Evry cedex, France

  • 7-25
    •   Srb10/Srb11 phosphorylates the Med2 mediator subunit both in vitro and in vivo.
    Magnus Hallberg, Gennady Polozkov, Stefan Bjorklund
    Medical Biochemistry, Umea University, n.a., Umea, S-901 87, Sweden (gennady.polozkov@medchem.umu.se)

  • 7-26
    •   The role of Set1p-catalyzed Histone H3K4 methylation.
    Vincent Geli (1), Merce Pamblanco (2), Pierre-Marie Dehe (1), Pierre Luciano (1), julie Sollier (1), Vicente Tordera (2), Daniele Monnier (1), Regine Lebrun (1)
    (1) LISM, CNRS, 31 ch Joseph Aiguier, Marseille, 13402, France (geli@ibsm.cnrs-mrs.fr); (2) University of Valencia, Faculty of Biology 46100 Burjassot, Spain

  • 7-27
    •   The role of UV-induced de novo protein synthesis in nucleotide excision repair is transcriptional-dependent in S. cerevisiae.
    Nisreen Al-Moghrabi, Ibtehagj Al-Sharif, Abdelilah Aboussekhra
    BMR, KFSH and RC, Takassosy, Riyadh, 11211, Saudi Arabia (nisreen_al_moghrabi@hotmail.com)

  • 7-28
    •   The nuclear actin-related proteins Arp7 and Arp9 form a dimeric module that cooperates with architectural proteins for chromatin remodeling.
    Heather Szerlong, Anjanabha Saha, Bradley R. Cairns
    Oncological Sciences, University of Utah, 2000 Circle of Hope, Salt Lake City, UT 84112, USA (Heather.Szerlong@HCI.Utah.edu)

  • 7-29
    •   Mechanistic analysis of RNA polymerase III hyper-processivity.
    Roberto Ferrari, Claudio Rivetti, Giorgio Dieci
    Dept. Biochem. and Mol. Biol., University of Parma, Area delle Scienze, Parma, 43100, Italy (gdieci@unipr.it)

  • 7-30
    •   A two-hybrid interaction map of the Mediator of transcription activation.
    Benjamin Guglielmi (1), Nynke van Berkum (2), Franck Holstege (2), Michel Werner (1)
    (1) Laboratoire de Physiogénomique, Bât. 144, CEA/Saclay, F-91191 Gif-sur-Yvette cedex, France (guglielm@mattieu.saclay.cea.fr); (2) Department of Physiological chemistry, UMC Utrecht, PO box 85060, 3508 AB Utrecht, Netherlands.

  • 7-31
    •   Iwr1: A novel, conserved RNAPII-interacting protein implicated in transcriptional elongation in Saccharomyces cerevisiae and Drosophila melanogaster.
    Nira Datta (1), Nevan Krogan (1), Gerard Cagney (1), Andrew Emili (1), Jack Greenblatt (1), Ali Shilatifard (2), Steve Buratowski (3)
    (1) Medical Genetics, University of Toronto, 112 College Street, Toronto, M5G 1L6, Canada (niradatta@hotmail.com); (2) St. Louis University, Biochemistry Room 157, 1402 S. Grand Blvd., St. Louis, MO, USA, 63104; (3) Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Bldg. C1, Room 210, 240 Longwood Avenue, Boston, MA 02115, USA.

    Poster Session 8 - RNA maturation and function

  • 8-1
    •   Evolution of the NMD pathway.
    Michael Culbertson, Amanda Ford, Eric Neeno-eckwall, Robert Zinkel, Wei Zheng, Qiaoning Guan
    Genetics and Molecular Biology, University of Wisconsin, 1525 Linden Dr., Madison, WI 53706, USA (mrculber@wisc.edu)

  • 8-2
    •   Mutations in genes of Saccharomyces cerevisiae encoding pre-mRNA splicing factors cause cell cycle arrest.
    Orna Dahan, Martin Kupiec
    Mol. Micro. and Biotech., Tel Aviv University, Ramat Aviv, Tel Aviv, 69978, Israel (ornad@post.tau.ac.il)

  • 8-3
    •   5' end formation of the linear plasmid mRNAs.
    Vaclav Vopalensky, Martin Pospisek
    Dep. Genetics and Microbiology, Charles University, Vinicna 5, Prague 2, 128 44, Czech Republic (vasek@natur.cuni.cz)

  • 8-4
    •   mRNA stability in yeast strains impaired in 5'-mRNA cap synthesis.
    Michaela Svobodova, Tomas Masek, Martin Pospisek
    Dept. Genetics & Microbiology, Charles University, Vinicna 5, Prague 2, 128 44, Czech Republic (svmichaela@hotmail.com)

  • 8-5
    •   Transfer RNA modifications that alter +1 frameshifting in general fail to affect -1 frameshifting.
    Jaunius Urbonavicius (1), Guillaume Stahl (2), Jerome Durand (1), Samia Ben Salem (3), Qiang Qian (1), Philip Farabaugh (3), Glenn Björk (1)
    (1) Dept. of Molecular Biology, Umeå University, University Campus, Umeå, 90781, Sweden (Jaunius.Urbonavicius@molbiol.umu.se); (2) CNRS, LMGM - IBCG, 118 route de Narbonne, 31062 Toulouse, France; (3) Department of Biological Sciences and Program in Molecular and Cell Biology, University of Maryland Baltimore County, Baltimore, MD 21250, USA

  • 8-6
    •   Addressing of RNA into yeast and human mitochondria: mechanisms and biomedical significance.
    Ivan Tarassov (1), Olga Kolesnikova (2), Francine Goltzene (1), Clarisse Becker (1), Robert Martin (1), Nina Entelis (1)
    (1) FRE 2375 CNRS, 21 rue Rene Descartes, 67084 Strasbourg, France (i.tarassov@ibmc.u-strasbg.fr); (2) Department of Molecular Biology, Moscow State University, Moscow 119899, Russia

  • 8-7
    •   Analysis of newly discovered ORFs interacting with KRR1.
    Iwona Karkusiewicz, Robert Gromadka, Joanna Rytka, Bozenna Rempola
    Genetics, IBB PAS, Pawinskiego 5A, Warsaw, 02-106, Poland (iwonak@ibb.waw.pl)

  • 8-8
    •   The tRNA 3' processing endonuclease of S. cerevisiae.
    Bettina Spaeth, Ingrid Schleyer, Anita Marchfelder
    Molecular Botany, Universitiy of Ulm, A.-Einstein-Allee 11, Ulm, 89069, Germany (bettina.spaeth@biologie.uni-ulm.de)

  • 8-9
    •   Molecular genetic analysis of candidate S. pombe pre-mRNA splicing factors.
    Piyush Khandelia, Usha Vijayraghavan
    Microbiology and Cell Biology, Indian Institute of Science, Dr. C.V. Raman Avenue, Bangalore, 560012, India (piyush@mcbl.iisc.ernet.in)

  • 8-10
    •   The multifunctional protein encoded by the mobile group II intron cob I1 in fission yeast mitochondria.
    Bernd Schaefer (1), Philip S. Perlman (2)
    (1) Biology IV (Microbiology), Aachen Technical University, Worringer Weg 1, Aachen, D-52056, Germany (bernd.schaefer@rwth-aachen.de); (2) Department of Molecular Biology, University of Texas, Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, USA

  • 8-11
    •   Searching the yeast genome for -1 frameshifting sites.
    Michaël Bekaert (1), Laure Bidou (1), Guillemette Duchateau-Nguyen (1), Isabelle Hatin (1), Jean-Pierre Rousset (1), Michel Termier (1), Alain Denise (2), Jean-Paul Forest (2) and Christine Froidevaux (2)
    (1) Institut de Génétique et Microbiologie, UMR CNRS 8621, Université Paris-Sud, 91405 Orsay Cedex, France (rousset@igmors.u-psud.fr); (2) Laboratoire de Recherche en Informatique, UMR CNRS 8623, Université Paris-Sud, 91405 Orsay Cedex, France

  • 8-12
    •   Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in pre-rRNA processing.
    Markus Buchhaupt, Dietmar Eschrich, Peter Kötter, Karl-Dieter Entian
    Institute of Microbiology, University of Frankfurt, Marie-Curie-Str. 9, Frankfurt am Main, 60439, Germany (buchhaupt@em.uni-frankfurt.de)

  • 8-13
    •   Functional characterization of 90S pre-ribosome components.
    Mercedes Dosil, Xose R. Bustelo
    Centro del Cancer, University of Salamanca/CSIC, Campus Unamuno, Salamanca, 37007, Spain (mdosil@usal.es)

  • 8-14
    •   Role of upstream AUGs and exoribonuclease Kem1p in the translational regulation.
    Jinmi Kim, Soonmee Jeon, Jeahee Kim
    Microbiology, Chungnam National University, Kung Dong 220, Taejeon, 305-764, South Korea (jmkim@cnu.ac.kr)

  • 8-15
    •   Use of the TAP method to characterise yeast splicing factors.
    Andrzej Dziembowski, Bertrand Seraphin
    CGM, CNRS, Avenue de la Terrass, Gif sur Yvette, 91198, France (Andrzej.Dziembowski@cgm.cnrs-gif.fr)

  • 8-16
    •   The rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes.
    Michael McAlear, Cristopher Wade, Mark Umbarger
    Mol. Biol. & Biochem., Wesleyan University, 237 Church St., Middletown, CT 06459, USA (mmcalear@wesleyan.edu)

  • 8-17
    •   A panoramic view of yeast non-coding RNA processing.
    Wen-Tao Peng, Mark Robinson, Sanie Mnaimneh, Nevan Krogan, Ger Cagney, Andrew Emili, Charlie Boone, Timothy Hughes
    BBDMR, University of Toronto, 112 College St., Toronto, ON M5G 1L6, Canada (t.hughes@utoronto.ca)

  • 8-18
    •   Functional Dissection of the Pop1p Subunit of Nuclear RNase P.
    Shaohua Xiao, Paul Good, Felicia Scott, Rebecca Nugent, Chatchawan Srisawat, David Engelke
    Dept. of Biological Chemistry, University of Michigan, 1301 E. Catherine St, Ann Arbor, MI 48109-0606, USA (paulgood@umich.edu)

  • 8-19
    •   Identification of genes important for biogenesis of tRNA Ser/CGA.
    Marcus J. O. Johansson, Anders S. Byström
    Dept. of Molecular Biology, Umeå University, Building 6L, Umeå, 901 87, Sweden (anders.bystrom@molbiol.umu.se)

  • 8-20
    •   RNA branching and debranching in the yeast Ty1 retrotransposon.
    Thomas Menees, Zhi Cheng
    Cell Biology and Biophysics, University of Missouri-KC, 5007 Rockhill Road, Kansas City, MO 64110, USA (meneest@umkc.edu)

  • 8-21
    •   Programmed frameshifting in Saccharomyces species: frameshifting evolved over 50 million years ago.
    Ana Raman, Emily Kramer, Carla Vesay, Philip Farabaugh
    Dept. of Biological Sciences, U of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA (farabaug@umbc.edu)

  • 8-22
    •   Transfer RNA modification enzymes in yeast: How many enzymes?
    Henri Grosjean
    CNRS, Laboratory of Structural Enzymology and Biochemistry, 1 ave de la Terrasse, Blg 34, F-91198 Gif-sur-Yvette, France (Grosjean@lebs.cnrs-gif.fr)

  • 8-23
    •   A nuclear protein in Schizosaccharomyces pombe with homology to the human tumour suppressor Fhit has decapping activity.
    Zivar Salehi (1), Lars Geffers (2), Cristina Vilela (2), Marina Ptushkina (2), Ralf Birkenhager (2), Karine Bertelot (2), Ian Hagan (3), John McCarthy (2)
    (1) Biology, The University of Guilan, Namjo, Rasht, 41335, Iran (geneticzs@yahoo.co.uk); (2) Department of Biomolecular Sciences Department of BMS UMIST, PO Box 88, Manchester M60 1QD, UK; (3) Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK

    Poster Session 9 - Protein biosynthesis, maturation, modification, degradation

  • 9-1
    •   Proteasome deubiquitinating activity - Intrinsic or associated?
    Adi Guterman, Michael Glickman
    Dept. of Biology, The Technion, I.T.T., Haifa, 32000, Israel (adigute@tx.technion.ac.il)

  • 9-2
    •   Lipid remodeling of glycosylphosphatidylinositol anchors in yeast Saccharomyces cerevisiae.
    Régine Bosson, Isabelle Guillas, Andreas Conzelmann
    Institute of Biochemistry, University of Fribourg, Ch. du Musée 5, Fribourg, 1700, Switzerland (regine.bosson@unifr.ch)

  • 9-3
    •   Nonsense mutations in the essential gene SUP35 Saccharomyces cerevisiae.
    Svetlana Chabelskaia (1), Michel Philippe (2), Galina Zhouravleva (1)
    (1) Department of Genetics and Selection, St. Petersburg State University, Universitetskaja nab., 7/9 199034, St. Petersburg, Russia (sveta@btc.bio.pu.ru); (2) UMR6061 CNRS Génétique et Développement, Université de Rennes I, 2 av. Pr. Léon Bernard, 35043 Rennes cedex, France

  • 9-4
    •   N-terminal domain of yeast eRF3 chain release factor is a negative cis-regulator of C-domain function.
    Kirill Osipov (1), Kirill Volkov (1), Igor Valouev (2), Ludmila Mironova (1)
    (1) Department of Genetics, St. Petersburg State University, Universitetskaya nab, St. Petersburg, 199034, Russia (kvom@mail.ru); (2) Institute of Experimental Cardiology, Cardiology Research Center, 3rd Cherepkovskaya Street, Moscow, 121552, Russia

  • 9-5
    •   Viable nonsense mutants for the essential gene SUP45 of Saccharomyces cerevisiae.
    Svetlana Moskalenko (1), Sergei Inge-Vechtomov (1), Michel Philippe (2), Galina Zhouravleva (1)
    (1) Genetics, St. Petersburg State University, Universitetskaya nab, St. Petersburg, 199034, Russian Federation (smoskalenko@hotmail.com); (2) Université de Rennes 1, CNRS UMR 6061, IFR 97, 2 av. Pr. Léon Bernard 35043 Rennes Cedex, France

  • 9-6
    •   Frameshift suppression by eRF3 inactivation in Saccharomyces cerevisiae.
    Sergey G. Inge-Vechtomov, Natalia A. Ryabinkova, Gennadiy V. Polozkov, Julia V. Sopova, Maria Savelova
    Genetics and Breeding, St. Petersburg State Universit, Universitetskaya 7/9, St. Petersburg, 199034, Russian Federation (inge@mailru.com)

  • 9-7
    •   Expression of K+-channel subunits in yeast and analysis of the subcellular distribution.
    Sarah Schwarzer, Hella Lichtenberg-Fraté, Jost Ludwig
    AG Molekulare Bioenergetik, Universität Bonn, Kirschallee 1, Bonn, 53115, Germany (uzscsl@uni-bonn.de)

  • 9-8
    •   An intron as a translational terminator in a prokaryotic/eukaryotic shuttle vector.
    Peter Bergquist, Moreland Gibbs, Rosalind Reeves
    Biological Sciences, Macquarie University, Herring Road, Sydney, 2109, Australia (peter.bergquist@mq.edu.au)

  • 9-9
    •   Glutathione synthetase is functional both as a homodimer and as a heterotetramer in the fission yeast Schizosaccharomyces pombe .
    Nadine Phlippen (1), Kurt Hoffmann (2), Klaus Wolf (1), Martin Zimmermann (1)
    (1) Institute of Biology IV, Aachen University, Worringer Weg, Aachen, 52056, Germany (Nadine.Phlippen@epost.de); (2) Institute of Biology VII, Aachen University, Worringer Weg, Aachen, 52056, Germany

  • 9-10
    •   The Npr1p kinase phosphorylates the N-terminal domain of amino acid permease Bap2p in response to rapamycin.
    Fumihiko Omura (1), Yukiko Kodama (2)
    (1) Institute for Liquor Products, Suntory Limited, 1-1-1, Wakayamadai, Shimamoto, Osaka, 618-8503, Japan (Fumihiko_Omura@suntory.co.jp); (2) Institute for Advanced Technology, Suntory Limited

  • 9-11
    •   Independent roles of free and proteasome-incorporated Rpn10.
    Noa Reis, Yulia Matyuhin, Arun Dakshinamurthy, Michael Glickman
    Biology, Technion, Kiryat hatechnion, Haifa, 32000, Israel (noareis@tx.technion.ac.il)

  • 9-12
    •   Members of the evolutionarily conserved PMT family of protein O-mannosyltransferases form distinct protein complexes among themselves.
    Verena Girrbach, Sabine Strahl
    Dep. of Cell Biology, University of Regensburg, Universitaetsstr. 31, Regensburg, D-93053, Germany (sabine.strahl-bolsinger@biologie.uni-regensburg.de)

  • 9-13
    •   Disulfide-bond formation in Schizosaccharomyces pombe : characterization of homologues to Saccharomyces cerevisiae Ero1p.
    Karina Kettner, Gerhard Rödel
    Institute for Genetics, Technische Universität Dresden, Mommsenstraße 13, Dresden, 01062, Germany (K_Kettner@hotmail.com)

  • 9-14
    •   HEL genes causing high expression lethality in proteasome mutant background are involved in apoptotic cell death, transcriptional activation and cell cycle control.
    Harish Karnam, Stefan Haefner, Iris Velten, Martin Ligr, Wolfgang Hilt
    Institut für Biochemie, Universität Stuttgart, Pfaffenwaldring 55, Stuttgart, 70569, Germany (hilt@po.uni-stuttgart.de)

  • 9-15
    •   The ubiquitin domain protein Ddi1 functions in DNA damage response mediated degradation of Ho endonuclease of yeast.
    Regina Tzirkin, Yelena Ivantsiv, Emilia Klyman, Dina Raveh
    Life Sciences, Ben Gurion University, P.O. Box 653, Beersheba, 84105, Israel (tzirkin@bgumail.bgu.ac.il)

  • 9-16
    •   Characterization of the 12 kDa acidic P1/P2 protein interaction sites in the yeast ribosomal stalk.
    Jorge Perez Fernandez, De-Yi Qiu, Miguel Remacha, Juan P.G. Ballesta
    Centro de Biologia Molecular, Universidad Autonoma de Madrid, Canto Blanco, Madrid, 28049, Spain (jpgballesta@cbm.uam.es)

  • 9-17
    •   The role of the Pre7 propeptide remnant in 20S proteasome assembly.
    Saravanakumar Iyappan, Wolfgang Heinemeyer
    Institut fuer Biochemie, Universitaet Stuttgart, Pfaffenwaldring 55, Stuttgart, 70569, Germany (iysara@po.uni-stuttgart.de)

  • 9-18
    •   Autophagy and intravacuolar membrane lysis in Saccharomyces cerevisiae.
    Ulrike D. Epple (1), Eeva-Liisa Eskelinen (2), Michael Thumm (1)
    (1) Institute of Biochemistry, University of Stuttgart, Pfaffenwaldring 55, Stuttgart, 70569, Germany (ulrike.epple@web.de); (2) Institute of Biochemistry, University of Kiel, Olshausenstr. 40, 24098 Kiel, Germany

  • 9-19
    •   Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae : a genome-wide screen identifies eight novel GID-genes and indicates the existence of two degradation pathways.
    Jochen Regelmann (1), Thomas Schüle (1), Frank S. Josupeit (1), Jaroslav Horak (2), Matthias Rose (3), Karl-Dieter Entian (3), Michael Thumm (1), Dieter H. Wolf (1)
    (1) Institute of Biochemistry, University of Stuttgart, Pfaffenwaldring 55, Stuttgart, 70569, Germany (jochen.regelmann@brs-concepts.de); (2) Czech Academy of Sciences, Institute of Physiology, Videnska 1083, 14220 Prague, Czech Republic; (3) Institute of Microbiology, Johann Wolfgang Goethe-University Frankfurt, Marie-Curie-Straße 9, 60439 Frankfurt, Germany

  • 9-20
    •   Analysis of the dynamics of protein turnover in the yeast proteome.
    June Petty (1), Julia Hayter (2), Duncan H. L. Robertson (2), Julie Pratt (2), Deborah Ward (2), Andrew Hayes (1), Simon Gaskell (3), Robert J. Beynon (2), Stephen G. Oliver (1)
    (1) School of Biological Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK (j.petty@man.ac.uk); (2) Department of Veterinary Preclinical Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZJ UK; (3) Michael Barber Centre for Mass Spectrometry, Department of Chemistry, UMIST, Manchester M60 1QD, UK.

  • 9-21
    •   Nat3p and Mdm20p are required for function of yeast NatB N-terminal acetyltransferase and of actin and tropomyosin.
    Bogdan Polevoda (1), Thomas Cardillo (1), Timothy Doyle (2), Gurrinder Bedi (3), Fred Sherman (1)
    (1) Biochemistry and Biophysics, University of Rochester, 601 Elmwood Ave., Rochester, NY 14642, USA (Bogdan_Polevoda@urmc.rochester.edu); (2) Xenogen Corporation, Alameda, CA 94501 USA; (3) Center for Oral Biology, University of Rochester, Rochester, NY 14642 USA

  • 9-22
    •   RAD6 dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO.
    Carsten Hoege, Boris Pfander, George-Lucian Moldovan, George Pyrowolakis, Stefan Jentsch
    Dept of Molecular Cell Biology, Max Planck Inst. of Biochem., Am Klopferspitz 18a, Martinsried, 82152, Germany (pfander@biochem.mpg.de)

  • 9-23
    •   Characterization of genes involved in N-glycosylation in Yarrowia lipolytica.
    Yunkyoung Song (1), Hyun Ah Kang (2), Jeong-Yoon Kim (1)
    (1) Microbiology, Chungnam National University, Yuseong, Daejeon, 305-764, Korea (songyunkyoung@hanmail.net); (2) Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea

  • 9-24
    •   Molecular analysis of HpOCH1 and HpHOC1, two novel genes involved in cell wall integrity and N-linked glycosylation in the methylotrophic yeast Hansenula polymorpha.
    Moo Woong Kim (1), Jeong-Yoon Kim (2), Yun Wi Oh (1), Sang Ki Rhee (1), Hyun Ah Kang (1)
    (1) Mebatolic Engineering Lab, KRIBB, Yusong-gu, Daejeon, 305-600, Korea (hyunkang@kribb.re.kr); (2) Dep. of Microbiology, Chungnam National University, Daejeon 305-764, Korea

  • 9-25
    •   Rpn7p is required for the assembly of the lid of the yeast 26S proteasome.
    Erika Isono (1), Yasushi Saeki (2), Hideyoshi Yokosawa (2), Akio Toh-e (1)
    (1) Dept. of Biological Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-0033, Japan (ss16353@mail.ecc.u-tokyo.ac.jp); (2) Grad. School of Pharmaceutical Sciences, Hokkaido University, N-12, W-6, Kita-Ku, Sapporo, Hokkaido 060-0812, Japan

  • 9-26
    •   Characterization of the N-terminal acetyl transferase NatB.
    Robert Svensson, Anders Blomberg
    Gsteborg University, CMB-Microbiology, Box 462, Gsteborg, 405 30, Sweden (robert.svensson@gmm.gu.se)

  • 9-27
    •   Determination of chain-types of polyubiquitin chains tagged to proteins.
    Yasushi Saeki (1), Yoko Tayama (1), Akio Toh-e (2), Hideyoshi Yokosawa (1)
    (1) Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan (saeki@pharm.hokudai.ac.jp); (2) Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan

  • 9-28
    •   Identification of a new UDP-Glc:glycoprotein glucosyltransferase in the yeast Yarrowia lipolytica.
    Anna Babour, Jean marie Beckerich, Claude Gaillardin
    Génétique cellulaire, INA /INRA/CNRS, Ina-PG, Thiverval Grignon, 78850, France (babour@grignon.inra.fr)

  • 9-29
    •   Analysis of two autophagy deficient mutants of the yeast Yarrowia lipolytica.
    Iryna Parshyna, Carola Hoffmann, Gerold Barth
    Institut für Mikrobiologie, Technische Universität Dresden, Mommsenstr. 13, Dresden, 01062, Germany (ip3@rcs.urz.tu-dresden.de)

  • 9-30
    •   Budding yeast Fdr2 and Fdr3, two novel FHA-domain containing RING-finger proteins, form a potential E3 ubiquitin ligase complex involved in cytokinesis.
    Michael Schwab, Mike Tyers
    S Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Ave, Toronto, ON M5G 1X5, Canada (schwab@mshri.on.ca)

  • 9-31
    •   In vivo evidence that translation initiation factor 3 functions in ribosomal scanning and GCN4 translational control.
    Klaus H. Nielsen, Bela Szamecz, Antonina Jivotovskaya, Leos Valasek, Alan G. Hinnebusch
    LGRD, NIH, 6 Center Drive, Bethesda, Md 20892, USA (nielsenk@mail.nih.gov)

  • 9-32
    •   Solid-state fermentation specific induction of two protease genes under substrate limitation in Aspergillus Oryzae.
    Rob te Biesebeke (1), Nick van Biezen (2), Willem de Vos (1), Kees van den Hondel (2), Peter Punt (2)
    (1) WCFS, Wageningen Centre for Food Sciences, P.O. Box 557, 6700AN Wageningen The Netherlands; (2) TNO Nutrition and Food Research Institute, P.O. Box 360, Utrechtseweg 48, 3700AJ Zeist The Netherlands (biesebeke@voeding.tno.nl)

  • 9-33
    •   On the engineering of a genetic code change in yeast.
    Raquel Silva (1), Ana C. Gomes (1), Jorge S. Santos (1), Michel Perrot (2), Hélian Boucherie (2), Manuel A. S. Santos (1)
    (1) Center for Cell Biology, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal (rsilva@bio.ua.pt); (2) Institut de Biochimie et Génétique Cellulaires, UPR CNR S9026, 1, Rue Camille Saint-Saëns, 33700, Bordeaux Cedex, France

  • 9-34
    •   Proteome-wide biochemical screen to identify ubiquitination targets of yeast ubiquitin ligase enzymes.
    Bart Kus, Catherine Caldon, Aled Edwards
    Medical Biophysics, University of Toronto, 112 College, Toronto, M5W1L6, Canada (bart.kus@utoronto.ca)

  • 9-35
    •   Cym1p, a novel endoprotease in Saccharomyces cerevisiae involved in the biosynthesis of CCK-22.
    Lars Jønson, Jens F. Rehfeld, Anders H. Johnsen
    Dept. of Clinical Biochemistry, Rigshospitalet, Blegdamsvej 9, Copenhagen, DK-2100, Denmark (lajo@biobase.dk)

  • 9-36
    •   Selectivity of benzimidzaole nucleoside analogues in regulation of protein kinases phosphorylating yeast's 60S ribosomal proteins.
    Piotr Zien (1), Olga Abramczyk (1), Maria Bretner (2), David Shugar (2), Ryszard Szyszka (1)
    (1) Molecular Biology, Catholic University of Lublin, Al. Krasnicka 102, Lublin, 20-718, Poland (piotr.zien@kul.lublin.pl); (2) Institute of Biochemistry and Biophysics Polish Academy of Science, Pawinskiego 5a, 02-106 Warszawa, Poland

  • 9-37
    •   Yeast Cu-Zn superoxide dismutase as an inhibitor of ribosomal P proteins phosphorylation.
    Rafal Zielinski, Marek Pilecki, Konrad Kubinski, Piotr Zien, Ryszard Szyszka
    Mollecular Biology, Catholic University of Lublin, Al. Krasnicka 102, Lublin, 20-718, Poland (rziel@kul.lublin.pl)

  • 9-38
    •   Different sets of tRNA are required for decoding the 62 sense codons of mRNA of Eukarya, Bacteria and Archaea.
    Henri Grosjean (1), Christian Marck (2)
    (1) Laboratoire d'Enzymologie et Biochimie Structurales, 1 ave de la Terrasse, Bat.34, F-91198 Gif-sur-Yvette, France. (Grosjean@lebs.cnrs-gif.fr); (2) Service de Biochimie et de Génétique Moléculaire, Bat.144, CEA/Saclay, F-91191, Gif-sur-Yvette, France.

  • 9-39
    •   Divergent co-evolution of a CDK substrate and its cyclin.
    Tsvia Gildor, Revital Shemer, Daniel Kornitzer
    Dept. of Microbiology, Technion Faculty of Medicine, 2, Efron St., Haifa, 31096, Israel (Gildor@tx.technion.ac.il)

  • 9-40
    •   Characterization of the regulatory pathway of Gcn4 degradation in C. albicans.
    Avigail Atir-Lande, Tsvia Gildor, Daniel Kornitzer
    Dept. of Microbiology, Technion Faculty of Medicine, 2, Efron St., Haifa, 31096, Israel (Lande@tx.technion.ac.il)

  • 9-41
    •   Glucose dependant negative translational control of the heterologous expression of the preS2 HBV antigen in yeast.
    Istabrak Borchani-Chabchoub, Raja Mokdad-Gargouri, Ali Gargouri
    Lab. Génét. Mol. Eucaryotes, Centre Biotech. de Sfax, Sokra, Sfax, K3038, Tunisia (Faouzi.Gargouri@cbs.rnrt.tn)

  • 9-42
    •   Lhs1p - an assistance partner for Kar2p in translocation and protein refolding in the endoplasmic reticulum.
    Anton Shmelev, and Marja Makarow
    Institute of Biotechnology, University of Helsinki, PL56, Helsinki, HY-00014, Finland (anton.shmelev@helsinki.fi, marja.makarow@helsinki.fi)

    Poster Session 10 - Molecular dissection of signal transduction systems

  • 10-1
    •   New monomeric variants of DsRed.
    Daniel E. Strongin, Brooke J. Bevis, Benjamin S. Glick
    Molec. Genetics and Cell Bio., University of Chicago, 920 E. 58th St., Chicago, IL 60637, USA (destrong@uchicago.edu)

  • 10-2
    •   Nbs1 is required for the S-phase DNA damage checkpoint in fission yeast.
    Sasirekha Sivakumar, Nick Rhind
    Biochemitry, UMass Med School, 364 Plantation, Worcester, MA 01605, USA (nick.rhind@umassmed.edu)

  • 10-3
    •   Connection between RNA processing and amino acid signalling in the FGM pathway in Saccharomyces cerevisiae.
    Ole Lagatie, Inge Holsbeeks, Lisbeth Maurissen, Johan M. Thevelein
    Lab. of Molecular Cell Biology, KULeuven, Kasteelpark Arenberg 31, Leuven, 3001, Belgium (ole.lagatie@bio.kuleuven.ac.be)

  • 10-4
    •   Characterization of Krh1, a novel signal transduction component of the protein kinase A pathway in Saccharomyces cerevisiae.
    Ruud Gelade, David Nauwelaers, Johan M. Thevelein
    Lab of Molecular Cell Biology, Katholieke Universiteit Leuven, Kasteelpark Arenb 31, Leuven-Heverlee, 3001, Belgium (ruud.gelade@bio.kuleuven.ac.be)

  • 10-5
    •   Activation-dependent degradation of the MEKK Ste11p.
    Nicolas Dard (1), Frank van Drogen (2), Matthias Peter (1)
    (1) Institute of Biochemistry, ETH Honggerberg, Building HPM, Zurich, 8093, Switzerland (nicolas.dard@bc.biol.ethz.ch); (2) The Scripps Research Institute, Department of Molecular Biology, La Jolla, CA 92037 USA

  • 10-6
    •   Ste5: the history of a protein that wanted to be more than a simple scaffold.
    Alma Savinon-Tejeda, Lindsay Garrenton, Jeremy Thorner
    Molecular and Cell Biology, University of California, Room 455 LSA, Berkeley, CA 94720-3200, USA (asavinon@uclink.berkeley.edu)

  • 10-7
    •   Methylglyoxal levels: an internal signal for the genetic response?
    Jaime Aguilera, Jose Antonio Prieto
    Biotechnology, IATA-CSIC, Pol., Paterna, Valencia Spain (jaguilera@iata.csic.es)

  • 10-8
    •   Effects of the PHO pathway on phosphate-regulation of PKA targets in Saccharomyces cerevisiae.
    Erwin Swinnen, Joris Winderickx, Johan Thevelein
    department of Biology, Katholieke Universiteit Leuven, Kasteelpark Arenb 31, Heverlee-Leuven, 3001, Belgium (erwin.swinnen@bio.kuleuven.ac.be)

  • 10-9
    •   Regulation of Ca 2+ -dependant flocculation by the Saccharomyces cerevisiae transcriptional regulator Mss11p.
    Michael C. Bester, Cornelius J. Franken, Florian F. Bauer
    Department of Agriculture, Inst. for Wine Biotechnology, Victoriastreet, Stellenbosch, 7600, South Africa (iwbt32@maties.sun.ac.za)

  • 10-10
    •   Identification of new transcription factors specifically regulated by PKA and the Sch9 protein kinase in Saccharomyces cerevisiae.
    Johnny Roosen (1), Elisabetta Cameroni (2), Claudio De Virgilio (2), Harmen J. Bussemaker (3), Johan M. Thevelein (4), Joris Winderickx (1)
    (1) Molecular Cell Biology, K.U. Leuven, Kasteelpark Arenberg, Leuven, 3001, Belgium (johnny.roosen@bio.kuleuven.ac.be); (2) Département de Biochimie Médicale, Centre Médical Universitaire, 1, rue Michel-Servet, CH - 1211 Genève 4, Switzerland; (3) Department of Biological Sciences and Columbia Genome Center, Columbia University, 1212 Amsterdam Avenue, MC 2441 - New York, NY 10027, USA; (4) Departement Moleculaire Microbiologie, Vlaams Interuniversitair Instituut voor Biotechnologie, Kasteelpark Arenberg 31, B-3001, Leuven-Heverlee, Flanders, Belgium

  • 10-11
    •   Proteins of the PalA/Rim20/AIP1/Alix family bind to a YPXL/I motif.
    Olivier Vincent
    Dept of Molecular Microbiology, C.I.B.- C.S.I.C., Velázquez, 144, Madrid, 28006, Spain (ovincent@cib.csic.es)

  • 10-12
    •   Signal propagation in the Saccharomyces cerevisiae invasive growth pathway.
    Dagmar M. Truckses, Joshua E. Bloomekatz, Jeremy Thorner
    Molecular & Cell Biology, Univ. of California, Berkeley, Rm. 401, Barker Hall, Berkeley, CA 94720-3202, USA (jeremy@socrates.berkeley.edu)

  • 10-13
    •   A MAP kinase docking Motif is required for Rck2p to relay lethality of PBS2DD and for multicopy suppressor activity of Rck2p K201R.
    Linghuo Jiang (1), Shuang Niu (1), Katrina Clines (1), Carol Chrestensen (1), Daniel Burke (2), Thomas Sturgill (1)
    (1) Pharmacology, University of Virginia, Jefferson Park Ave, Charlottesville, VA 22908, USA (tws7w@virginia.edu); (2) Molecular Genetics and Biochemistry, University of Virginia, Jefferson Park Ave, Charlottesville, VA 22908, USA

  • 10-14
    •   Is there a role for PP1 and/or PP2A in glucose induced signaling in Saccharomyces cerevisiae?
    Ils Somers, Stefaan Wera, Johan M. Thevelein
    Biology, Botany and Microbiology, Kasteelpark Arenberg, Heverlee, 3001, Belgium (ils.somers@bio.kuleuven.ac.be)

  • 10-15
    •   Gap1: novel sensor for amino acids in Saccharomyces cerevisiae?
    Inge Holsbeeks, Griet Van Zeebroeck, Ole Lagatie, Monica C.V. Donaton, Johan M. Thevelein
    Lab of Molecular Cell Biology, K.U.Leuven, Kasteelpark Arenb 31, Leuven, 3001, Belgium (Inge.Holsbeeks@bio.kuleuven.ac.be)

  • 10-16
    •   Active Snf1 protein kinase inhibits expression of the Saccharomyces cerevisae HXT1 glucose transporter gene.
    Lidia Tomás-Cobos, Pascual Sanz
    Insto. Biomedicina de Valencia, CSIC, Jaume Roig,11, Valencia, E-46010, Spain (ltomas@ibv.csic.es)

  • 10-17
    •   Glucose-induced cAMP signalling and its sugar phosphorylation requirement in Saccharomyces cerevisiae.
    Christine Oesterhelt (*, 1), Katrien Pardons (*, 1), Joris Winderickx (1), Johan Thevelein (2)
    (*) Equally contributing authors; (1) Physiology and Biochemistry of Plants and Micro-organisms, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31, Heverlee, Belgium (katrien.pardons@bio.kuleuven.ac.be); (2) Molecular Microbiology, VIB, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31, Heverlee, Belgium

  • 10-18
    •   Tfs1p, a member of the PEBP family, inhibits the Ira2p but not the Ira1p Ras GTPase activating protein in Saccharomyces cerevisiae.
    Hélène Bénédetti (1), Hélène Chautard (1), Gaelle Massard (1), Françoise Schoentgen (1), Michel Jacquet (2), Nicole Bureaud (1)
    (1) CBM- UPR4301, CNRS, Rue Charles Sadron, Orléans, 45071, France (benedett@cnrs-orleans.fr); (2) Laboratoire Information Génétique et Développement, Institut de Génétique et Microbiologie, UMR CNRS-Université 8621, Bât 400, Université Paris-Sud, 91405 Orsay Cedex, France

  • 10-19
    •   Saccharomyces cerevisiae Cdc25: a still puzzling GEF.
    Renata Tisi, Fiorella Belotti, Francesco Brunetti, Sonia Colombo, Enzo Martegani
    Biotecnologie e Bioscienze, Università di Milano Bicocca, P.zza della Scienza, Milano, 20126, Italy (enzo.martegani@unimib.it)

  • 10-20
    •   Is inositol triphosphate a second messenger in yeast?
    Renata Tisi (1), Stefaan Wera (2), Joris Winderickx (2), Johan M. Thevelein (2), Enzo Martegani (1)
    (1) Dip. Biotecnol. e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza, Milan, 20126, Italy (renata.tisi@unimib.it); (2) Plantkunde en Microbiologie, K.U. Leuven, Kasteelpark Arenberg 31, B-3001 Heverlee, Belgium

  • 10-21
    •   Gis4p is a novel component of ion homeostasis in the yeast Saccharomyces cerevisiae.
    Ye Tian, Xiaoming Bao, Markus J. Tamas, Stefan Hohmann
    CMB, Göteborg University, Medicinaregatan 9E, Göteborg, 41390, Sweden (tian.ye@gmm.gu.se)

  • 10-22
    •   Ammonia, a pivotal S. cerevisiae nutrient, positively and negatively regulates retrograde and NAD-glutamate dehydrogenase gene expression, respectively.
    Jennifer J. Tate, Terrance G. Cooper
    Molecular Sciences, University of Tennessee, 858 Madison Ave., Memphis, TN 38163, USA (jtate@utmem.edu)

  • 10-23
    •   Pak1 associates with and activates Snf1 kinase.
    Martin C. Schmidt, Nandita Nath, Rhonda McCartney
    Mol. Genetics & Biochemistry, Univ. of Pittsburgh, School of Medicine, Pittsburgh, PA 15261, USA (mcs2@pitt.edu)

  • 10-24
    •   Molecular mechanisms governing oxygen sensing and heme signaling in Saccharomyces cerevisiae.
    Li Zhang, Thomas Hon, Hee Chul Lee, Changgui Lan
    Biochemistry, NYU School of Medicine, MSB329, 550 1st Ave., New York, NY 10016, USA (li.zhang@med.nyu.edu)

  • 10-25
    •   Regulation of Tec1p by Kss1p and Fus3p.
    Stefan Brückner, Tim Köhler, Gerhard Braus, Hans-Ulrich Mösch
    Mol. Microbiology and Genetics, Georg-August-University, Grisebachstr. 8, Göttingen, D-37077, Germany (sbrueck@gwdg.de)

  • 10-27
    •   Role played by Ras2 in the regulation of the cAMP/PKA pathway.
    Sonia Colombo (1), Daniela Ronchetti (1), Joris Winderickx (2), Johan Thevelein (2), Enzo Martegani (1)
    (1) Biotecnologie e Bioscienze, Università Milano Bicocca, Piazza della Scienza, Milano, 20126, Italy (Sonia.Colombo@unimib.it); (2) Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Kasteelpark Arenberg 31 B-3001 Leuven-Heverlee Flanders, Belgium

  • 10-28
    •   The Schizosaccharomyces pombe git1 gene of the glucose/cAMP signaling pathway.
    Richard Kao, Eric Morreale, Charles S. Hoffman
    Biology Department, Boston College, 140 Commonwealth Ave, Chestnut Hill, MA 02467, USA (hoffmacs@bc.edu)

  • 10-29
    •   The role of phosphoribosyl pyrophosphate synthetase (Prs) in cell wall integrity signalling transduction in Saccharomyces cerevisiae.
    Ke Wang (1), Lilian M. Schweizer (1), Jürgen J Heinisch (2), Michael Schweizer (1)
    (1) School of Life Sciences, Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS, United Kingdom (Ke.Wang@hw.ac.uk); (2) Fachbereich Biologie/Chemie, Universität Osnabrück, D-49076 Osnabrück, Germany

  • 10-30
    •   Dynamics of the components in the amino acid sensing pathway of Saccharomyces cerevisiae.
    Nadine Eckert-Boulet (1), Birgitte Regenberg (1), Allan Stensballe (2), Ole Nørregaard Jensen (2), Jens Nielsen (1)
    (1) CPB, building 223, BioCentrum-DTU, Søltofts Plads, Kgs Lyngby, 2800, Denmark (neb@biocentrum.dtu.dk); (2) Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark

  • 10-31
    •   Tap42 mediates rapamycin-induced transcriptional effects by activating PP2A phosphates.
    Katrin Duevel, Lisa Schneper, James Broach
    Molecular Biology, Princeton University, Washington Road, Princeton, NJ 08544, USA (kduvel@molbio.princeton.edu)

  • 10-32
    •   The role of the Puf-protein Mpt5 in HSP12 expression.
    Joakim Norbeck
    Swegene Proteomics Centre, Göteborg University, Medicinaregatan 7B, Göteborg, 41390, Sweden (joakim.norbeck@sahlgrenska.gu.se)

  • 10-33
    •   Glucose regulation of HXT gene expression by the transcription factor Rgt1.
    Sabire Ozcan, Amber L. Mosley, Jaganathan Lakshmanan, Bishwa K. Aryal
    Mol. & Cellular Biochemistry, University of Kentucky, 800 Rose Street, Lexington, KY 40536, USA (sozcan@uky.edu)

  • 10-34
    •   Inositol pyrophosphates control nitrogen, phosphate and salt-regulated genes.
    Evelyne Dubois, Fabienne Vierendeels, Mohamed El Alami, Francine Messenguy
    Microbiology, Institut de Recherches IRMW, Av. Emile Gryzon, 1, Brussels, 1070, Belgium (fanarg@ulb.ac.be)

  • 10-35
    •   Functional study of fungal HOG1 and human related p38 alpha, JNK1, and ERK5 genes in Saccharomyces cerevisiae.
    Tomas Ureña-Muñoz, Blanca Eisman, Federico Navarro-Garcia, Rebeca Alonso-Monge, Cesar Nombela, Jesus Pla
    Dpt. Microbiologia II, Univ. Complutense de Madrid, Pz. de Ramon y Cajal, Madrid, E-28040, Spain (fnavarro@farm.ucm.es)

  • 10-36
    •   Protease regulation in the yeast Yarrowia lipolytica.
    Sylvie Blanchin-Roland, Brigitte Tréton, Claude Gaillardin
    Génétique moléculaire, INRA/CNRS/INA-PG, CBAI, Thiverval-Grignon, F-78850, France (blanchin@grignon.inra.fr)

  • 10-37
    •   Mkp1 and Mkp2, two MAPKAP-kinase homologues in Schizosaccharomyces pombe, interact with the MAP kinase Sty1.
    Eva Asp, Per Sunnerhagen
    Cell and Molecular Biology, Göteborgs Universitet, Medicinaregatan 9C, Göteborg, 405 30, Sweden (eva.asp@molbio.gu.se)

  • 10-38
    •   Study of the temperature sensitivity and respiratory deficiency of pho85 mutants of Saccharomyces cerevisiae.
    Yulia Popova (1), Anastasiya Fisikova (2), Elena Sambuk (2)
    (1) Molecular Microbiology, VIB, KUL, Kasteelpark Arenberg, Leuven-Heverlee, B-3001, Belgium (yulia.popova@bio.kuleuven.ac.be); (2) Biological Research Institute of St.-Petersburg State University, Laboratory of Biochemical Genetics, St. Petersburg, Russia

  • 10-39
    •   Deficiency of PKC1 activity affects glycerol metabolism in Saccharomyces cerevisiae.
    Rogelio L. Brandão, Luciano G. Fietto, Kátia N. Gomes, Ieso M. Castro, Suzy M. A. C. Freitas, Thiago M. Pais
    DEFAR/EF/NUPEB, Federal University Ouro Preto, Campus M. Cruzeiro, Ouro Preto, MG 35.400-000, Brazil (rlbrand@nupeb.ufop.br)

  • 10-40
    •   Yeast osmosensor Sln1 and plant cytokinin receptor Cre1 respond to changes in turgor pressure.
    Vladimir Reiser, Desmond Raitt, Haruo Saito
    Dana-Farber Cancer Institute, Harvard Medical School, 44 Binney St., Boston, MA 02115, USA (vladimir_reiser@dfci.harvard.edu)

  • 10-41
    •   Intracellular inorganic phosphate and polyphosphate are the signals of the PHO pathway in Saccharomyces cerevisiae.
    Choowong Auesukaree (1), Tomoyuki Homma (1), Hidehito Tochio (2), Masahiro Shirakawa (2), Yoshinobu Kaneko (1), Satoshi Harashima (1)
    (1) Department of Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan (choo2@bio.eng.osaka-u.ac.jp); (2) Department of Science of Biological Supramolecular Systems, Yokohama City University, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan

  • 10-42
    •   Mutant MRT4 is capable of restoring normal growth to the yvh1 protein phosphatase disruptant mutant of Saccharomyces cerevisiae.
    Satya Nugroho, Naoko Sakumoto, Satoshi Harashima
    Department of Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan (satya2@bio.eng.osaka-u.ac.jp)

  • 10-43
    •   Reciprocal regulation between the dual-specificity protein phosphatase Msg5 and the MAP kinase Slt2 in the yeast cell integrity pathway.
    Marta Flández, Inmaculada Cosano, Eva Tapia, César Nombela, Humberto Martín, María Molina
    Microbiología II Farmacia, Universidad Complutense, Pza Ramón y Cajal, Madrid, 28040, Spain (humberto@farm.ucm.es)

  • 10-44
    •   Downregulation of the HOG MAP kinase pathway during yeast osmotic adaptation.
    Bodil Nordlander (1), Peter Gennemark (2), Markus J Tamás (1), Dag Wedelin (2), Stefan Hohmann (1)
    (1) CMB/Microbiology, Göteborg University, Box 462, Göteborg, 40530, Sweden (bodil.nordlander@gmm.gu.se); (2) Computing Science, Chalmers University of Technology, Eklandagatan 86, 412 96 Göteborg, Sweden

  • 10-45
    •   Investigation of the link between the osmotic stress response and control of energy metabolism in baker's yeast, Saccharomyces cerevisiae.
    Marcus Krantz, Stefan Hohmann
    CMB/Microbiology, Göteborg University, Box 462, Göteborg, 40530, Sweden (marcus.krantz@gmm.gu.se)

  • 10-46
    •   Analysis of Ste50 function in cell signalling between the activated G-protein and the MAPKK kinase Ste11 of S. cerevisiae.
    Claudia Hopp, Cornelis P. Hollenberg, Massoud Ramezani-Rad
    Institute for Microbiology, Heinrich-Heine University, Universitätsstr.1, Düsseldorf, 40225, Germany (claudia.hopp@uni-duesseldorf.de)

  • 10-47
    •   Cyclophilin A is nuclear localized and controls the sporulation gene program of Saccharomyces cerevisiae.
    Miguel Arévalo-Rodríguez, Joseph Heitman
    Mol. Genetics and Microbiology, Duke University Medical Center, Research Drive, Durham, 27710, USA (miguel@duke.edu)

  • 10-48
    •   Phosphorylation-regulated degradation of the yeast Ssk1p response regulator by the ubiquitin-proteasome system.
    Naoto Sato (1), Hiroyuki Kawahara (2), Akio Toh-e (3), Tatsuya Maeda (1)
    (1) IMCB, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo, 113-0032, Japan (ss97200@mail.ecc.u-tokyo.ac.jp); (2) Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan; (3) Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan

  • 10-49
    •   Osmostress induced transcription by Hog1-mediated recruitment of the RNA Pol II.
    Francesc Posas (1), Paula M. Alepuz (1), Eulàlia de Nadal (1), Meritxell Zapater (1), Gustav Ammerer (2)
    (1) CEXS, Universitat Pompeu Fabra, Dr. Aiguader 80, Barcelona, 08003, Spain (francesc.posas@cexs.upf.es); (2) Department of Biochemistry and Molecular Cell Biology, Ludwig Boltzmann-Forschungsstelle. University of Vienna, A1030 Vienna, Austria.

  • 10-50
    •   Mechanism of signal transduction by the Snf3 and Rgt2 glucose sensors.
    Hisao Moriya, Mark Johnston
    Genetics, Washington University, 660 S. Euclid, St. Louis, MO 63119, USA (hisaom@genetics.wustl.edu)

  • 10-51
    •   Asi1p, Asi2p, and Asi3p are integral nuclear membrane proteins that negatively modulate transcription of amino acid permease genes.
    Arezou Zargari, Mirta Boban, Claes Andréasson, Per O. Ljungdahl
    Yeast Molecular Biology, Ludwig Inst. Cancer Research, BOX 240, Stockholm, 17177, Sweden (azar@licr.ki.se)

  • 10-52
    •   Identification of Elm1p as one of three upstream kinase for the Saccharomyces cerevisiae SNF1 complex.
    Catherine M. Sutherland (1), Simon A. Hawley (1), Rhonda R. McCartney (2), Anna Leech (2), Michael J. R. Stark (1), Martin C. Schmidt (1), D. Grahame Hardie (2)
    (1) School of Life Sciences, University of Dundee, MSI/WTB Complex, Dundee, DD1 5EH, UK (m.j.r.stark@dundee.ac.uk); (2) Department of Molecular Genetics and Biochemistry, University of Pittsburgh, Pennsylvania 15261, USA

  • 10-53
    •   Gpr1, a sugar sensing G protein coupled receptor.
    Sam Van de Velde, Katleen Lemaire, Johan Thevelein
    Molecular Cell Biology, KU Leuven, Kasteelpark Arenberg, Heverlee, 3001, Belgium

  • 10-54
    •   Quantitative RT-PCR as a tool to study dynamic expression response after glucose pulse to ethanol grown cells.
    Karin Otterstedt (1), Anders Stålberg (1), Christer Larsson (1), Stefan Hohmann (2), Lena Gustafsson (1)
    (1) Molecular Biotechnology, Chalmers University, Box 462, Göteborg, 405 30, Sweden (karin.otterstedt@molbiotech.chalmers.se); (2) Department of Cell and Molecular Biology, Göteborg University, Sweden

    Poster Session 11 - Surviving in lab and nature: stress responses

  • 11-1
    •   Molecular mechanisms regulating cadmium detoxification in Saccharomyces cerevisiae.
    Elis Eleutherio (1), Paula Adamis (2), Debora Gomes (2), Lilian Fragoso (2), Cristiano Riger (2), Anita Panek (2)
    (1) Department of Biochemistry, Institut of Chemistry, UFRJ, 21949-900, Rio de Janeiro, Brazil (eliscael@iq.ufrj.br); (2) Biochemistry -Inst. Chemistry, UFRJ, Brig. Trompowysk s/n, Rio de Janeiro RJ, 21949-900, Brazil

  • 11-2
    •   Protection against oxidation during dehydration of yeast.
    Elenilda Pereira (1), Anita Panek (1), Elis Eleutherio (2)
    (1) Biochemistry - Inst. Chemistry, UFRJ, Brig. Trompowysk s/n, Rio de Janeiro RJ, 21949-900, Brazil; (2) Department of Biochemistry, Institut of Chemistry, UFRJ, 21949-900, Rio de Janeiro, Brazil (eliscael@iq.ufrj.br)

  • 11-3
    •   Targeted inhibition of proteasome activity is essential for survival of yeast during stationary phase.
    Monika Bajorek (1), Dan Finley (2), Michael Glickman (1)
    (1) Biology, The Technion, I.I.T., Haifa, 32000, Israel (glickman@tx.technion.ac.il); (2) Dept. of Cell biology, Harvard Med. School 200 Longwood Ave. Boston MA 02115 USA

  • 11-4
    •   Pra1, a novel protein in S. cerevisiae, that regulates GCN4 levels independent of GCN2.
    Brian Luke (1), Matthias Gstaiger (2), Wilhelm Krek (2), Matthias Peter (1)
    (1) Biochemistry, ETH-Zürich, Hönggerberg, Zürich, 8093, Switzerland (brian.luke@bc.biol.ethz.ch); (2) Friedrich Miescher Institute, Maulbeerstrasse 66, 4058 Basel, Switzerland

  • 11-5
    •   Combination of Two Activating Mutations in One HOG1 Gene Forms Hyperactive Enzymes that Induce Growth Arrest.
    Gilad Yaakov, Michal Bell, David Engelberg
    Dept. of Biological Chemistry, Hebrew University of Jerusalem, Givat Ram, Jerusalem, 91904, Israel (gyaakov@pob.huji.ac.il)

  • 11-6
    •   Revealing the mechanism of HSP104 transcription initiation in the yeast S. cerevisiae.
    Melanie R. Grably, Ariel Stanhill, Osnat Tell, David Engelberg
    Dept. of Biological Chemistry, Hebrew University of Jerusalem, Givat Ram, Jerusalem, 91904, Israel (msgrably@mail.ls.huji.ac.il)

  • 11-7
    •   Osmotic stress resistant yeast Zygosaccharomyces rouxii - isolation of auxotrophic mutants and efficient transformation by electroporation.
    Lenka Pribylova, Hana Sychrova
    Dept. Membrane Transport, Inst. Physiology CzAcadSci, Videnska 1083, Prague 4, 142 20, Czech Republic (pribyll@biomed.cas.cz)

  • 11-8
    •   Yeast responses to stress induced by the herbicide 2,4-D: role of Pdr1p/Pdr3p- and Msn2p/Msn4p-regulated genes.
    Miguel Cacho Teixeira, Tânia Simões, Pedro Miguel Santos, Alexandra R. Fernandes, Isabel Sá-Correia
    CEBQ/Biological Sciences, Instituto Superior Técnico, Av. Rovisco Pais, Lisboa, 1049-001, Portugal (mnpct@ist.utl.pt)

  • 11-9
    •   Activation and significance of vacuolar H+-ATPase during yeast adaptation to stressful concentrations of the herbicide 2,4-D.
    Alexandra R. Fernandes, Paulo J. Durão, Pedro M. Santos, Isabel Sá-Correia
    Centre Biological Chem Eng, Instituto Superior Técnico, Av. Rovisco Pais, Lisbon, 1049-001, Portugal (pcxana@popsrv.ist.utl.pt)

  • 11-10
    •   HPV16-E6 expression in Saccharomyces cerevisiae: interaction with intrinsic proteins that alter normal cell functioning.
    Ana Pinto de Oliveira, Teresa Gonçalves
    Microbiology, Faculty of Medicine, Rua Larga, Coimbra, 3004-504, Portugal (apintooliveira@hotmail.com)

  • 11-11
    •   The Saccharomyces cerevisiae response to oxidative stress: integrative study on the role of the glyoxalase pathway.
    Ana Margarida Martins, Pedro Mendes
    Virginia Bioinf. Institute, Virginia Tech, 1880 Pratt Drive, Blacksburg, VA 24061, USA (amartins@vbi.vt.edu)

  • 11-12
    •   Effect of Rck2p on translation during oxidative stress in Sacharomyces cerevisiae.
    Tomas Masek (1), Swarnalatha Swaminathan (2), Martin Pospisek (1), Per Sunnerhagen (2)
    (1) Genetics and Microbiology, Faculty of Science, Vinicna 5, Prague, 128 44, Czech Republic (masek@natur.cuni.cz); (2) Department of Cell and Molecular Biology, Lundberg Laboratory, Göteborg University, Box 462, S-405 30 Göteborg, Sweden

  • 11-13
    •   Characterization of the function of YAP family members: involvement of Yap8p with arsenite- and arsenate-resistance mechanism.
    Regina Menezes, Catarina Amaral, Claudina Rodrigues-Pousada
    ITQB, Universidade Nova de Lisboa, Av. Republica - ITQB, Oeiras, 2781-901, Portugal (rmenezes@itqb.unl.pt)

  • 11-14
    •   Different colony morphotypes and domestication of wild Saccharomyces cerevisiae strains.
    Martin Kuthan, Vratislav Stovicek, Zdena Palkova
    Genetics and Microbiology, Charles University, Vinicna 5, Prague, 12844, Czech Republic (kuty@natur.cuni.cz)

  • 11-15
    •   Functional divergence of monothiol and dithiol glutaredoxins in Saccharomyces cerevisiae.
    María Micaela Molina (1), Gemma Bellí (1), María Teresa Rodríguez-Manzaneque (1), Per Sunnerhagen (2), Enrique Herrero (1)
    (1) Dept. Basic Medical Sciences, University of Lleida, Rovira Roure 44, Lleida, 25198, Spain (micaela.molina@cmb.udl.es); (2) Department of Cell and Molecular Biology, Göteborg University, S-40530 Göteborg, Sweden

  • 11-16
    •   Oxidative stress induces PKC1-MAP kinase activity which is required for cell survival.
    Felipe Vilella, Jordi Torres-Rosell, Enrique Herrero, María Angeles de la Torre-Ruiz
    Dept. Basic Medical Sciences, University of Lleida, Rovira Roure 44, Lleida, 25198, Spain (felip.vilella@cmb.udl.es)

  • 11-17
    •   A thiol-peroxydase is an H2O2 Receptor and Redox-Transducer in Gene Activation.
    Agnès Delaunay (1), Delphine Pflieger (2), Marie-Bénédicte Barrault (1), Joelle Vinh (2), Michel B. Toledano (1)
    (1) Laboratoire Stress Oxydants et Cancers, SBGM/DBJC, CEA-Saclay, 91191 Gif-sur Yvette. France (delaunay@jonas.saclay.cea.fr); (2) Laboratoire de Neurobiologie et Diversité Cellulaire, CNRS-UMR 7637, ESPCI, 10 rue Vauquelin, 75231 Paris Cedex 05. France

  • 11-18
    •   Regulation of YAP4 in response to osmotic and oxidative stress.
    Tracy Nevitt (1), Jorge Pereira (1), Dulce Azevedo (1), Paulo Guerreiro (2), Claudina Rodrigues-Pousada (1)
    (1) Stress and Genomics, ITQB, Av da Republica, Lisbon, 2780Oeiras, Portugal (tracy@itqb.unl.pt); (2) Escola Superior de Tecnologia da Saéde de Lisboa, R. José Carlos dos Santos 7, P-1700-256 Lisboa, Portugal

  • 11-19
    •   Trans-dominant mutations in two members of the GPR1/FUN34/YAAH protein family (YNR002C, YCR010C) effect acetic acid sensitivity in Saccharomyces cerevisiae.
    Margret Kuschel (1), Kristina Creuzburg (1), Stefan Hohmann (2), Gerold Barth (1)
    (1) Institute of Microbiology, Technical University Dresden, Mommsenstr. 13, Dresden, 01062, Germany (Kuschel@rcs.urz.tu-dresden.de); (2) Department of Cell and Molecular Biology/ Microbiology, Göteborg University, Box 462, S-40530 Göteborg, Sweden, Phone: +46-31-7732595, Fax: +46-31-7732599, (hohmann@gmm.gu.se)

  • 11-20
    •   A new yeast antioxidant co-factor of peroxiredoxins.
    Benoit Biteau, Michel B. Toledano
    LSOC, SBGM, DBJC, DSV, CEA Saclay, Gif sur Yvette, 91191, France (biteau@jonas.saclay.cea.fr)

  • 11-21
    •   The role of GSH1/MET1 and GSH2 genes in cadmium, chromate and methanol tolerance in methylotrophic yeast Hansenula polymorpha.
    Oleksandra Mahola (1), Vera Ubiyvovk (1), Taras Nazarko (1), Andrei Sibirny (1), Min Jeong Sohn (2), Hyun Ah Kang (1)
    (1) Molecular genetics and biotech, Institute of Cell Biology, Drahomanov St.,14/16, Lviv, 79005, Ukraine (o_mahola@yahoo.com); (2) Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea

  • 11-22
    •   Yeast as Biosensors. Using the Stress Response Elements to monitor water toxicity.
    Irit Marbach, Tamar Gilon, Ruth Cohen, David Engelberg
    Biological Chemistry, The Hebrew University, Givat Ram, Jerusalem, 91904, Israel (marbach@mail.ls.huji.ac.il)

  • 11-23
    •   Identification of salt stress responsive genes, encoding glycerol-3-phosphate dehydrogenase and putative ENA-ATPase in halophilic black yeast Hortaea werneckii.
    Alenka Gorjan, Ana Plemenitas
    Institute of Biochemistry, Medical Faculty, Vrazov trg 2, Ljubljana, SI-1000, Slovenia (alenka.gorjan@mf.uni-lj.si)

  • 11-24
    •   Control of gene expression by the yeast Hog1 MAPK.
    Eulàlia de Nadal (1), Paula M. Alepuz (1), Meritxell Zapater (1), Laura Casadomé (1), Gustav Ammerer (2), Francesc Posas (1)
    (1) Cell Signaling Unit, Universitat Pompeu Fabra (UPF), C/ Aiguader 80, Barcelona, 08003, Spain (eulalia.nadal@cexs.upf.es); (2) Department of Biochemistry and Molecular Cell Biology, Lutwig Boltzmann-Forschungsstelle. University of Vienna. Dr. Bohrgasse 9, 1030 Vienna, Austria

  • 11-25
    •   Role of the molecular chaperones Hsp82 and Cdc37p in the regulation of osmotic stress response.
    Xiaoxian Yang, Kick T.C. Maurer, Michiel Molanus, Willem H. Mager, Marco Siderius, Saskia M. van der Vies
    Dept. of Biochem. & Mol. Biol., Vrije Universiteit, De Boelelaan 1083, Amsterdam, 1081 HV, The Netherlands (xian@chem.vu.nl)

  • 11-26
    •   Isolation and characterisation of six new open reading frames that are important for stress resistance during fermentation in S. cerevisiae.
    Barbara Leyman (1), Sonia Colombo (2), Johan M. Thevelein (1), Patrick Van Dijck (1)
    (1) Lab. Mol. Cell Biology, K.U.L., Flemish Inst. Biotechnol. VIB, Kasteelp. Arenberg31, Leuven, B-3001, Belgium (Flanders) (patrick.vandijck@bio.kuleuven.ac.be); (2) present address: Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi Milano-Bicocca, Italy

  • 11-27
    •   Large-scale analysis of transcription factors localization under environmental stresses.
    Rosa M. Marion (1), Erin K. O'Shea (2)
    (1) Biochemistry and Biophysics, UCSF, 600 16th Street, San Francisco, CA 94143, USA (rmarion@itsa.ucsf.edu); (2) Howard Hughes Medical Institute, University of California - San Francisco, Department of Biochemistry & Biophysics, 600 16th Street, San Francisco, CA 94143-2240, USA

  • 11-28
    •   Evidence for the Yeast High Osmolarity Glycerol Mitogen Activated Protein Kinase Pathway (MAPK) regulating adaption to citric acid stress.
    Clare Lawrence, Peter Coote
    Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK (cll6@st-andrews.ac.uk)

  • 11-29
    •   Genomic analysis of the regulation of sorbic acid-inducible protein expression in spoilage yeast.
    Vasso Makrantoni, Peter Coote
    Biomolecular Sciences, University of St. Andrews, North Haugh, St. Andrews, KY169ST, U.K. (vm14@st-andrews.ac.uk)

  • 11-30
    •   SRG1, a Torulaspora delbrueckii gene that regulates growth and stress tolerance in yeast.
    Elena Aller-Arranz, Francisca Randez-Gil, Jose Antonio Prieto
    Biotechnology, Ito. de Agroquímica (CSIC), P.O.Box 73, Burjassot, 46100, Valencia, Spain (allere@iata.csic.es)

  • 11-31
    •   Organizing MAPK signaling in the osmostress response.
    Marco Siderius, Xiaoxian Yang, JanPaul Bebelman, Saskia vd Vies
    Biochemistry/Molecular Biology, De Vrije Universiteit, De Boelelaan 1083, Amsterdam, 1081HV, The Netherlands (Siderius@chem.vu.nl)

  • 11-32
    •   Molecular and physiological differences in wine yeast strains with different fermentative behaviour.
    Aurora Zuzuarregui, Marcel.lí Del Olmo
    Dept. Biochem. and Mol. Biol., Universitat de València, C/Dr. Moliner, 50, Burjassot (Val), 46100, Spain (Aurora.Zuzuarregui@uv.es)

  • 11-33
    •   Transcriptional response to acetaldehyde stress.
    Agustín Aranda, Marcel-lí Del Olmo
    Dept. Bioquímica i Biol. Mol., Universitat de València, Dr. Moliner, 50, Burjassot (Val), 46100, Spain (agustin.aranda@uv.es)

  • 11-34
    •   Osmotically induced GPD1 expression and glycerol translocation in Saccharomyces species.
    Philip White (1), Katherine Smart (1), Alan Kennedy (2)
    (1) School of BMS, Oxford Brookes University, Gipsy Lane, Headington, Oxford, OX3 0BP, UK (pawhite@brookes.ac.uk); (2) Scottish Courage Brewing Limited, Technical Centre, Sugar House Close, 160 Canongate, Edinburgh EH8 8DY, UK

  • 11-35
    •   The transcriptional response of ethanol-stressed yeast to the presence of acetaldehyde.
    Idris Mohammed (1), Grant Stanley (1), Paul Chambers (1), Peter Rogers (2)
    (1) School of Molecular Sciences, Victoria University, Hoppers Lane, Melbourne, 3030, Australia (Idris.Mohammed@research.vu.edu.au); (2) Carlton & United Breweries, 4-6 Southampton Crescent, Abbotsford, Vic., Australia, 3001

  • 11-36
    •   The global response to the antifungal drug caspofungin in yeast requires the cell integrity signalling pathway.
    Cristina Reinoso, Christoph Schüller, Manuela Schützer-Mühlbauer, Karl Kuchler
    Medical Biochemistry, Campus Vienna Biocenter, Dr. Bohr-Gasse 9/2, Vienna, A-1030, Austria (kaku@mol.univie.ac.at)

  • 11-37
    •   Deciphering the drug resistance regulation network in yeast with artificial transcription activators.
    Frédéric Devaux, Anca Lucau-Danila, Thierry Delaveau, Vivienne Fardeau, Gaelle Lelandais, Véronique Tanty, Immrich Hikkel, Claude Jacq
    LGM, ENS/CNRS UMR 8541, 46 rue d'Ulm, Paris, 75005, France (devaux@biologie.ens.fr)

  • 11-38
    •   Mechanism of UV sensitivity of Saccharomyces cerevisiae deleted in LSM1 gene.
    Tatana Spicakova, James Brown, Martin Brown
    Radiation Oncology, Stanford University, 269 Campus Drive W, Stanford, CA 94305, USA (tatanas@stanford.edu)

  • 11-39
    •   Chronological aging and protein oxidative damage.
    Gemma Reverter, Elisa Cabiscol, Jordi Tamarit, Joaquim Ros
    Ciencies Mediques Basiques, Facultat de Medicina, Rovira Roure, 44, Lleida, 25198, Spain (gemma.reverter@cmb.udl.es)

  • 11-40
    •   Functional analysis of the DAN gene family in Saccharomyces species.
    Stephen J. Lawrence (1), Alan I. Kennedy (2), Katherine A. Smart (1)
    (1) BMS, Oxford Brookes University, Gipsy Lane, Oxford, OX3 0BP, UK (Stephenjlawrence@hotmail.com); (2) Scottish Courage Brewing Limited, Technical Centre, Sugar House Close, 160 Canongate, Edinburgh EH8 8DY, UK

  • 11-41
    •   The response of Saccharomyces cerevisiae to heat stress.
    Femke I. C. Mensonides (1), J. Merijn Schuurmans (2), M. Joost Teixeira de Mattos (2), Klaas J. Hellingwerf (2), Stanley Brul (1)
    (1) Department of Molecular Biology & Microbial Food Safety, University of Amsterdam, Nwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands (mensonides@science.uva.nl); (2) Department of Microbiology, University of Amsterdam, Nwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands

  • 11-42
    •   Genome-Wide Characterisation of Tolerance of Oxidative Damage in Saccharomyces cerevisiae.
    Geoffrey W. Thorpe, Chii S. Fong, Chong Han Ng, Vincent J. Higgins, Ian W. Dawes
    Biotech. and Biomol. Sciences, University of NSW, Anzac Pde, Sydney, 2052, Australia (gorillainthemirror@yahoo.com)

  • 11-43
    •   Three protein-tyrosine phosphatase-like proteins are required for resistance to a lipid hydroperoxide.
    Nazif Alic (1), Vincent J. Higgins (1), Michael Breitenbach (2), Ian W. Dawes (1)
    (1) Ramaciotti Centre for Gene Function Analysis and School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia (n.alic@student.unsw.edu.au); (2) Departement of Genetics, University of Salzburg, Hellbrunnersts. 34, A-5020, Salzburg, Austria

  • 11-44
    •   A genetic approach to define factors influencing glutathione homeostasis in Saccharomyces cerevisiae.
    Gabriel G. Perrone (1), Chris M. Grant (2), Ian W. Dawes (1)
    (1) Ramaciotti Centre for Gene Function Analysis and School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia (g.perrone@unsw.edu.au); (2) Department of Biomolecular Sciences, UMIST M60 1QD, United Kingdom.

  • 11-45
    •   eIF2B is a target for fusel alcohol-dependent translational control.
    John Slaven (1), Christian Griffiths (1), Salma Ibrahimo (1), Alan Sachs (2), Mark Ashe (1)
    (1) Biomolecular Sciences, UMIST, Sackville Street, Manchester, M14 6UF, UK (J.Slaven@umist.ac.uk); (2) Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720. USA

  • 11-46
    •   A comprehensive approach to the study of salt tolerance in Debaryomyces hansenii.
    Jose Ramos (1), Vera Montiel (1), Catarina Prista (2), Raul Garcia-Salcedo (1), Maria C. Loureiro-Dias (2)
    (1) Microbiology, University of Cordoba, Campus Rabanales.C6, Cordoba, 14071, Spain (mi1raruj@uco.es); (2) DBEB, Instituto Superior de Agronomia, UTL, 1349-017 Lisbon, Portugal

  • 11-47
    •   Intracellular Sodium distribution in D. hansenii. Identification of the gene DhNHX1.
    Vera Montiel, Jose Ramos
    Microbiology, University of Cordoba, Campus Rabanales.C6, Cordoba, 14071, Spain (g52modiv@uco.es)

  • 11-48
    •   A comprehensive approach to the study of salt tolerance in Debaryomyces hansenii.
    Jose Ramos (1), Vera Montiel (1), Catarina Prista (2), Raul Garcia-Salcedo (1), Maria C. Loureiro-Dias (2)
    (1) Microbiology, University of Cordoba, campus Rabanales.C6, Cordoba, 14071, Spain (mi1raruj@uco.es); (2) DBEB Instituto Superior de Agronomia 1349-017 Lisboa Portugal

  • 11-49
    •   Intracellular sodium distribution in the halotolerant yeast Debaryomyces hansenii. Identification of the DhNHX1 gene.
    Vera Montiel, Jose Ramos
    Microbiology, University of Cordoba, Campus Rabanales C6, Cordoba, 14071, Spain (g52modiv@uco.es)

  • 11-50
    •   Trehalose metabolism in Saccharomyces cerevisiae during rehydration and wine fermentation.
    Maite Novo, Gemma Beltran, M. Jesés Torija, Montse Poblet, J. Manuel Guillamón, Nicolas Rozès, Albert Mas
    Biochemistry and Biotechnology, Universitat Rovira i Virgili, Ramon y Cajal 70, Tarragona, 43005, Spain (manm@fq.urv.es)

  • 11-51
    •   The Pkc1p MAP kinase pathway triggers oxidative stress-induced destruction of the yeast C-type cyclin.
    Randy Strich (1), Todd Cohen (2), Elizabeth Krasely (1)
    (1) Cell and Developmental Biology, Fox Chase Cancer Center, 7701 Burholme Ave, Philadelphia, PA 19111, USA (r_strich@fccc.edu); (2) Department of Molecular Biology, Duke Univ. Durham, NC, USA

  • 11-52
    •   NADP-Glutamate Dehydrogenase activity in the Halotolerant Yeast Debaryomyces hansenii under hyperosmotic conditions.
    Luisa Alba-Lois (1), René Cárdenas (1), Claudia Segal (1), Beatriz Rodarte (1), Victor Valdés (1), Alexander De Luna (2), Alicia González (2)
    (1) Biología Celular, Facultad de Ciencias, U.N.A.M., Circuito Exterior, CU, México, DF, 04510, Mexico (lal@hp.fciencias.unam.mx); (2) Instituto de Fisiología Celular, U.N.A.M.

  • 11-53
    •   Yeast response to chromium.
    Yannick Pereira, Gilles Lagniel, Jean Labarre
    S.B.G.M., CEA-Centre de Saclay, Bat. 144, Gif-sur-Yvette, 91191, France (pereira@matthieu.saclay.cea.fr)

  • 11-54
    •   Transcriptome and proteome analysis of Saccharomyces cerevisiae under amino acid starvation.
    Malte Kleinschmidt, Oliver Valerius, Olav Grundmann, Gerhard Braus
    Molecular Microbiology & Genet, Microbiology & Genetics, Grisebachstr. 8, Goettingen, 37077, Germany (ovaleri@gwdg.de)

  • 11-55
    •   Functional analysis of the middle segment of Hsp90.
    Bin Hu (1), Philippe Meyer (2), Chrisostomos Prodromou (2), Chunyan Liao (1), Barry Panaretou (1), Peter W. Piper (3), Laurence H. Pearl (2)
    (1) Division of Life Sciences, King's College London, 150 Stamford Street, London, SE1 9NN, UK (bin.hu@kcl.ac.uk); (2) Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London SW3 6JB, UK; (3) Department of Biochemistry and Molecular Biology, University College London, WC1E 6BT, UK

  • 11-56
    •   Osmotolerance - a biotechnologically important characteristic of the non-conventional yeast Arxula adeninivorans.
    Erik Böer, Peggy Knobloch, Karen Dlubatz, Thomas Wartmann, Gotthard Kunze
    Molekulare Zellbiologie, IPK-Gatersleben, Corrensstr. 3, Gatersleben, D-06466, Gatersleben, Germany (boeer@ipk-gatersleben.de)

  • 11-57
    •   The Hog1 MAP kinase is activated by oxidative stress in the pathogenic fungus Candida albicans.
    Federico Navarro-Garcia, Rebeca Alonso-Monge, Cesar Nombela, Jesus Pla
    Microbiologia II, Univ. Complutense de Madrid, Pz. de Ramon y Cajal, Madrid, E-28040, Spain (fnavarro@farm.ucm.es)

  • 11-58
    •   Expression of a mammalian aquaporin confers osmosensitivity on the yeast Saccharomyces cerevisiae.
    Johan Hagström (1), Roslyn Bill (2), Kristina Hedfalk (2), Stefan Hohmann (1)
    (1) Department of Cell and Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden (johan.hagstrom@gmm.gu.se); (2) CMB, Microbiology, Medecinaregatan 9C, Göteborg, 41390, Sweden

  • 11-59
    •   Regulation of redox homeostasis in yeast.
    Eleanor W. Trotter, Chris M. Grant
    Biomoecular Sciences, UMIST, Sackville Street, Manchester, M60 1QD, UK (e.trotter@umist.ac.uk)

  • 11-60
    •   The role of Met4 in maintaining glutathione homeostasis via the regulation of glutathione biosynthesis.
    Glen L. Wheeler, Chris M. Grant
    Biomolecular Sciences, UMIST, The Mill, Manchester, M60 1QD, UK (G.Wheeler@umist.ac.uk)

  • 11-61
    •   Transcriptional response during recovery of Saccharomyces cerevisiae cells from hydrogen peroxide stress.
    Marta Marques (1), Vítor Costa (1), Dominik Mojzita (2), Maria Amélia Amorim (1), Teresa Almeida (1), Stefan Hohmann (2), Pedro Moradas-Ferreira (1)
    (1) MCA, IBMC, R. Campo Alegre, 823, Porto, 4150-180, Portugal (vcosta@ibmc.up.pt); (2) Department of Cell and Molecular Biology, Goteborg University, Sweden

  • 11-62
    •   Occurrence and Functionality of Cold Shock Genes In Saccharomyces species.
    Jessica P.R. Leclaire (1), Alan Kennedy (2), Katherine A. Smart (1)
    (1) School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Oxford, OX3 0BP, UK; (2) Scottish Courage Brewing Limited, Technical Centre, Sugarhouse Close, 160 Canongate, Edinburgh, UK. (Jleclaire@brookes.ac.uk)

  • 11-63
    •   Differential transcription activation patterns of QDR2 (ORF YIL121w) and QDR1, encoding yeast plasma membrane proteins of the DHA12 drug efflux family required for quinidine and ketoconazole resistance.
    Rita C. Vargas, Miguel C. Teixeira, Alexandra R. Fernandes, Sandra Tenreiro, Isabel Sá-Correia
    Centro de Eng. Biol. Química, Instituto Superior Técnico, Av. Rovisco Pais, Lisbon, 1049-001, Portugal (ritacv@ist.utl.pt)

  • 11-64
    •   Sphingolipid signalling in stress resistance and chronological life-span in Saccharomyces cerevisiae : the role of Isc1p.
    Teresa Almeida (1), Marta Marques (1), Maria Amélia Amorim (2), Paula Ludovico (3), Pedro Moradas-Ferreira (2), Manuela Côrte-Real (4), Vítor Costa (2)
    (1) MCA, IBMC, R. Campo Alegre, 823, Porto, 4150-180, Portugal (trcunha@ibmc.up.pt); (2) IBMC and ICBAS, Universidade do Porto; (3) Instituto de Ciências da Vida e da Saéde, Universidade do Minho; (4) Centro de Biologia, Universidade do Minho

  • 11-65
    •   Identification and characterization of novel calcineurin substrates.
    Victoria L. Heath, Nicola Burrows-Brown, Martha S. Cyert
    Biological Sciences, Stanford University, 371 Serra Mall, Palo Alto, CA 94305, USA (vheath@stanford.edu)

  • 11-66
    •   Long-term development of Saccharomyces cerevisiae colonies: Changes in stress factors.
    Libuse Vachova (1), Frederic Devaux (2), Helena Kucerova (1), Marketa Ricicova (3), Zdena Palkova (3)
    (1) Institute of Microbiology, CAS, Videnska 1083, Prague 4, Czech Republic; (2) Laboratoire de Génétique Moléculaire, CNRS 8541, Ecole Normale Supérieure, 46 rue d'Ulm, Paris, France; (3) Dept. Genet. Microbiol., Charles University, Vinicna 5, Prague, 12844, Czech Republic (zdenap@natur.cuni.cz)

  • 11-67
    •   A role of multidrug resistence transporters in elimination of intracellular stresses.
    Libuse Vachova, Karel Sigler, Helena Kucerova
    Cell. Mol. Microbiol., Institute of Microbiology CAS, Videnska 1083, Prague, 14220, Czech Republic (vachova@biomed.cas.cz)

  • 11-68
    •   Characterization of the yeast stress response to chitosan by genomic transcript analysis.
    Anna Zakrzewska, Andre Boorsma, Stanley Brul, Klaas Hellingwerf, Frans M. Klis
    SILS Microbiology, University of Amsterdam, Nieuwe Achtergracht, Amsterdam, 1018WV, The Netherlands (zakrzews@science.uva.nl)

  • 11-69
    •   Activation of Heat shock trnacription factor Hsf1 by Snf1-dependent phosphorylation under glucose starvation conditions.
    Ji-Sook Hahn, Dennis Thiele
    Biological Chemistry, University of Michigan, 1301 Catherine Rd, Ann Arbor, MI 48109, USA (hahnjs@umich.edu)

  • 11-70
    •   TOR and PKA signalling pathways converge on a new PAS kinase family member.
    Ivo Pedruzzi, Frédérique Dubouloz, Elisabetta Cameroni, Nicolas Hulo, Claudio De Virgilio
    Dép. de Biochimie Médicale, CMU, 1 rue Michel-Servet, Geneva, CH-1211, Switzerland (Ivo.Pedruzzi@medecine.unige.ch)

  • 11-71
    •   Molecular analysis of host-fungal interactions during Candida albicans infections.
    Caroline Barelle, Claire Manson, Susan Budge, Neil Gow, Alistair Brown
    Aberdeen Fungal Group, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK (c.j.barelle@abdn.ac.uk)

  • 11-72
    •   Molecular analysis of the hypoxia induced ADH2-promoter in the respiratory yeast Pichia stipitis.
    Volkmar Passoth (1), Marita Cohn (2), Bernd Schäfer (3), Bärbel Hahn-Hägerdal (4), Ulrich Klinner (3)
    (1) Department of Microbiology, SLU, Box 7025, Uppsala, S-750 07, Sweden (Volkmar.Passoth@mikrob.slu.se); (2) Department of Cell and Organism Biology (Molecular Genetics), Lund University, Sölvegatan 35, S-223 62 Lund, Sweden; (3) Institut für Biologie IV (Mikrobiologie), RWTH Aachen, Worringer Weg, D-56056 Aachen, Germany; (4) Applied Microbiology, Lund University, P.O. Box 124, S-221 00 Lund, Sweden

  • 11-73
    •   Mechanisms of heavy metal tolerance in the fission yeast Schizosaccharomyces pombe.
    Claudia Simm, Stephan Clemens
    Stress and Developmental Biol., Institute of Plant Biochem., Weinberg 3, Halle/Saale, 06120, Germany (csimm@ipb-halle.de)

  • 11-74
    •   Role of carotenoids in stress response of red yeast.
    Jana Pokorna, Radka Koci, Ivana Marova, Michaela Drabkova, Monika Knoppova
    Faculty of Chemistry, Brno Technical University, Purkynova 118, Brno, 612 00, Czech Republic (janca.p@post.cz)

  • 11-75
    •   Kluyveromyces lactis killer protein (zymocin) is activated by calcium and induces calcium influx in Saccharomyces cerevisiae.
    Hiroko K. Kitamoto (1), Andreea Cunita (2), Tokichi Miyakawa (2)
    (1) National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602 (kitamoto@affrc.go.jp); (2) Dept. of Molecular Biotechnology, Grad. School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8526, Japan

  • 11-76
    •   Comprehensive expression analysis of genetic responses in yeast cells to low temperature using microarray.
    Takehiko Sahara, Takako Goda, Satoru Ohgiya
    Res. Inst. Biol. Res. Funct., Nat. Inst. Adv. Ind. Sci. Tech, 2-17-2-1 Tsukisamu, Sapporo, 062-8517, Japan (t-sahara@aist.go.jp)

  • 11-77
    •   Dihydroxyacetone kinases in Saccharomyces cerevisiae are involved in detoxification of dihydroxyacetone.
    Mikael Molin (1), Joakim Norbeck (2), Anders Blomberg (1)
    (1) CMB Microbiology, Göteborg University, Medicinaregatan 9C, Göteborg, S-413 90, Sweden (mikael.molin@gmm.gu.se); (2) Swegene Proteomics Center, Göteborg University, Medicinaregatan 7B, S-413 90 Göteborg, Sweden

  • 11-78
    •   The killer toxin of the halotolerant yeast Candida nodaensis.
    Sónia Silva (1), Cristina Aguiar (1), Paula Veríssimo (2), Euclides Pires (2), Cândida Lucas (1)
    (1) Biologia, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal (clucas@bio.uminho.pt); (2) CNC - Lab. de Biologia Molecular e Biotecnologia, Universidade de Coimbra, Portugal

  • 11-79
    •   Identification of regulatory elements in the Saccharomyces cerevisiae GPD1 promoter with distinct function during adaptation to or steady-state growth in NaCl.
    Homan Alipour, Peter Eriksson, Anders Blomberg
    Microbiology, Cell and Molecular Biology, Medicinaregatan 9C, Göteborg, SE-405 30, Sweden (homan.alipour@gmm.gu.se)

  • 11-80
    •   FK506 sensitizes yeast and mammalian cells to high osmolarity through inactivation of the Hog1 and p38 MAP kinases: Involvement of the GCN pathway.
    Carlos J Rodriguez-Hernandez (1), Isabel Sanchez-Perez (2), Rosario Gil-Mascarell (1), Abigail Rodriguez-Afonso (1), Armando Torres (1), Rosario Perona (2), Jose R. Murguia (1)
    (1) Research Unit, Hospital Universitario Canaria, Ofra s/n-La Cuesta, La Laguna, 38320, Spain (carodri@ull.es); (2) Instituto de investigaciones Biomédicas (CSIC), C/ Arturo Duperier 4, 28029-Madrid, Spain

  • 11-81
    •   The Schizosaccharomyces pombe LYS7 gene is involved in copper metabolism.
    Aysegul Topal Sarikaya, Cagatay Tarhan
    Biology, Istanbul University, Vezneciler, Istanbul, 34459, Turkey (atopal@istanbul.edu.tr)

  • 11-82
    •   Whole genome transcription analysis and metabolome analysis of yeast stress response during very high gravity ethanol fermentation.
    Rasmus Devantier (1), Britta Scheithauer (2), Lisbeth Olsson (2)
    (1) Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby and Starch & Brewing, Applied Discovery, Research & Development, Novozymes A/S, Laurentsvej 51-53, DK-2880 Bagsvaerd, Denmark (rd@biocentrum.dtu.dk); (2) Center for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Lyngby

  • 11-83
    •   A Saccharomyces cerevisiae mutant, tolerant to multiple stress conditions affected in the general stress response pathway.
    Adriana Garay-Arroyo, Isadora Clark, Ricardo Peimbert, Jose Luis Reyes, Fernando Lledias, Alejandra A. Covarrubias
    Plant Molecular Biology, UNAM, Av. Universidad#2001, Cuernavaca, 62100, Mexico (adriana@ibt.unam.mx)

  • 11-84
    •   Comparative study of the adaptation of Saccharomyces cerevisiae to weak acids and membrane perturbing agents employed as food preservatives.
    Catarina Resende, André Boorsma, László van den Hoek, Frans Klis, Stanley Brul
    SILS, University of Amsterdam, Nw Achtergracht 166, Amsterdam, 1018 WV, The Netherlands (resende@science.uva.nl)

  • 11-85
    •   Rck1 and Rck2 MAPKAP kinases mediate oxidative stress resistance from the HOG pathway.
    Elizabeth Bilsland-Marchesan, Swarnalatha Swaminathan, Claes Molin, Anna Ramne, Per Sunnerhagen
    Cell and Molecular Biology, Göteborg University, P.O. Box 462, Göteborg , S-405 30, Sweden (Per.Sunnerhagen@molbio.gu.se)

  • 11-86
    •   The Cu,Zn-superoxide dismutase of Sacharomyces cerevisiae is required for resistance to hyperosmosis.
    Fernando Lledías (1), Adriana Garay-Arroyo (1), Wilhelm Hansberg (2), Alejandra A. Covarrubias (1)
    (1) Biología Molecular de Plantas, IBT/UNAM, Av. Universidad 2001, Cuernavaca, Mor., 62250, Mexico (flledias@ibt.unam.mx); (2) Departamento de Bioquímica, IFC/UNAM, Ciudad Universitaria, México D.F., Mexico

  • 11-87
    •   Role of cAMP signalling in yeast antifungal tolerance.
    Pooja Jain, Thomas Edlind
    Microbiology and Immunology, Drexel Univ Col of Med, 2900 Queen Lane, Philadelphia, PA 19129, USA (edlind@drexel.edu)

  • 11-88
    •   A complex network of efflux pumps and transcription factors is reponsible for diazaborine resistance in yeast.
    Eva Wehrschütz-Sigl, Helmut Jungwirth, Hermine Schein, Sandra Schmid, Helmut Bergler, Gregor Högenauer
    IMBM/Mikrobiologie, Karl-Franzens Universität, Universitätsplatz 2, Graz, 8010, Austria (sigle@uni-graz.at)

  • 11-89
    •   DNA microarray analysis reveals new aspects in diazaborine action and resistance.
    Brigitte Pertschy, Claudia Mikula, Helmut Bergler, Gregor Högenauer
    IMBM, Karl-Franzens-University Graz, Universitätsplatz 2, Graz, 8010, Austria (brigitte.pertschy@uni-graz.at)

  • 11-90
    •   Genomics-based identification of a new yeast mitochondrial heat shock-induced protein that is a functional ortholog to a mammalian kidney disease gene.
    Amy Trott, Kevin Morano
    Microbiology/Molecular Genetics, University of Texas-Houston, 6431 Fannin St., Houston, TX 77030, USA (Amy.Trott@uth.tmc.edu)

  • 11-91
    •   Salt sensitivity in respiratory deficient mutants of Kluyveromyces lactis.
    Barbara Neglia, Emmanuela Marchi, Iliana Ferrero, Paola Goffrini
    Department of Genetics, University of Parma, Parco Scienze 11/A, Parma, 43100, Italy (iferrero@unipr.it)

  • 11-92
    •   Resistance of Saccharomyces cerevisiae to toxic lignocellulose-derived aromatic compounds.
    Linda Björklund (1), Simona Larsson (2), Carlos Martín (3), Ilona Sárvári Horváth (4), Björn Alriksson (5), Leif J. Jönsson (5)
    (1) AstraZeneca R&D Mölndal, SE-431 83 Mölndal, Sweden; (2) STFI, P.O. Box 5604, SE-114 86 Stockholm, Sweden; (3) Department of Chemistry and Chemical Engineering, University of Matanzas, 44 740 Matanzas, Cuba; (4) Department of Chemical Reaction Engineering, Chalmers University of Technology, SE-412 96 Göteborg, Sweden and Biochemistry, Division for Chemistry, Karlstad University, SE-651 88 Karlstad, Sweden; (5) Biochemistry, Division for Chemistry, Karlstad University, SE-651 88 Karlstad, Sweden (Leif.Jonsson@kau.se)

  • 11-93
    •   Acidic ribosomal proteins under stress conditions.
    Samuel Zinker, Alicia Ochoa, Ivonne Olivares, Lilia Palma
    Genética y Biología Molecular, CINVESTAV (IPN), Av. Ins. Polite. Nac, Distrito Federal, 07360, México (szruzal@hotmail.com)

  • 11-94
    •   Regulation of D-arabitol and glycerol accumalation by Candida albicans in response to environmental stresses.
    Gerald Kayingo, Brian Wong
    Internal Medicine, Yale University, 950 Campbell Ave, West Haven, CT 06516, USA (gerald.kayingo@yale.edu)

  • 11-95
    •   HOG signaling pathway in halophilic black yeast Hortaea werneckii.
    Ana Plemenitas (1), Alenka Gorjan (1), Nina Gunde-Cimerman (2), Martina Turk (3)
    (1) Institute of Biochemistry, Madical Faculty, UL, Vrazov trg 2, Ljubljana, SI-1000, Slovenia (ana.plemenitas@mf.uni-lj.si); (2) Biotechical Faculty, Department of Biology, Vecna pot 111, SI-1000 Ljubljana, Slovenia; (3) Biotecnical Faculty, Department of Biology, Vecna pot 111, SI-1000 Ljubljana, Slovenia

  • 11-96
    •   Galactose uptake deficiency of a Kluyveromyces lactis snf1 mutant is suppressed by KHT3, a new galactose transporter gene.
    Christian Wiedemuth, Karin D. Breunig
    Institute of Genetics, University of Halle, Weinbergweg 10, Halle, D-06099, Germany (breunig@genetik.uni-halle.de)

  • 11-97
    •   Transcriptional activation of metalloid tolerance genes requires the yeast AP-1-like proteins Yap1p and Yap8p.
    Markus J. Tamás (1), Robert Wysocki (2), Dindial Ramotar (3)
    (1) Cell and Molecular Biology, Göteborg University, Box 462, Göteborg, 405 30, Sweden (markus.tamas@gmm.gu.se); (2) Institute of Microbiology, Wroclaw University, Poland; (3) Guy-Bernier Research Centre, University of Montreal, Quebec, Canada

  • 11-98
    •   Saccharomyces cerevisiae Dlt1/Ymr126c is required for growth at low temperature.
    Marie celeste De Jesus Ferreira, Stefan Hohmann
    Cell and Mol Bio/Microbiology, Göteborg University, Box 462, Göteborg, 40530, Sweden (rubief@hotmail.com)

  • 11-99
    •   The phospholipase B homolog, Plb1, is a mediator of osmotic stress response and nutrient-dependent repression of sexual differentiation in the fission yeast, Schizosaccharomyces pombe.
    Peirong Yang (1), Hongyan Du (1), Charles Hoffman (2), Stevan Marcus (1)
    (1) Dept. of Molecular Genetics, UT M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA (smarcus@mdacc.tmc.edu); (2) Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467 USA

  • 11-100
    •   Cellular Response to Arsenic.
    Michael Thorsen, Markus J. Tamás
    Cell and Molecular Biology, Göteborg University, Medicinaregatan 9C, Göteborg, 41390, Sweden (michael.thorsen@gmm.gu.se)

  • 11-101
    •   Investigation of the molecular function of YPL201c in the regulation of glycerol production in Saccharomyces cerevisiae.
    Katarina Granath, Lennart Adler
    CMB-Microbiology, University of Göteborg, Medicinaregatan 9E, Göteborg, S-413 90, Sweden (katarina.granath@gmm.gu.se)

  • 11-102
    •   Yeast response to chromium on genome and proteome level.
    Peter Raspor (1), Borut Poljsak (2), Miklos Pesti (3), Stojan Plesnièar (2)
    (1) Food Science and Technology Department, Chair of Biotechnology, Biotechnical faculty, Jamnikarjeva 101, 1000 Ljubljana, Slovenia (peter.raspor@bf.uni-lj.si); (2) Polytechnic Nova Gorica, School of Environmental Science, Vipavska 13, 5000 Nova Gorica, Slovenia; (3) Department of General and Environmental Microbiology, Faculty of Sciences, University of Pecs, P.O.B. 266, H-7601 Pecs, Hungary

  • 11-103
    •   Expression of two dithiol glutaredoxins, Grx1 and Grx2, in Schizosaccharomyces pombe.
    Woo-Hyun Chung, Jung-Hye Roe
    School of Biological Sciences, Institute of Microbiology, Seoul National Univ., Seoul, 151-742, Korea (gsheph23@hanmir.com)

  • 11-104
    •   Comparative analysis of cadmium tolerance and glutathione biosynthesis in different strains of the metylotrophic yeast Hansenula polymorpha.
    Min Jeong Sohn (1), Se-Jeong Kim (1), Chul Ho Kim (1), Sang Ki Rhee (1), Vira M. Ubiyvovk (2), Andrei A. Sibirny (2), Hyun Ah Kang (1)
    (1) Metabolic Engineering, KRIBB, Yuseong-gu, Daejeon, 305-600, Korea (picorna@mail.kribb.re.kr); (2) Institute of Cell Biology, Drahomanov Str. 14/16, Lviv 79005, Ukraine.

    Poster Session 12 - Control and engineering of cellular metabolism

  • 12-1
    •   Loss of fermentative capacity in baker's yeast can be partly explained by reduced glucose uptake activity.
    Sergio Rossell, Coen C. van der Weijden, Arthur L. Kruckeberg, Barbara M. Bakker, Hans V. Westerhoff
    Molecular Cell Physiology, Vrije Universiteit, de Boelelaan 1085, Amsterdam, 1081 HV, The Netherlands (sergio@bio.vu.nl)

  • 12-2
    •   Metabolic regulation of heme biosynthesis.
    Piotr Pawlowski, Marta Hoffman, Monika Gora, Joanna Rytka, Piotr Zielenkiewicz
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland (martah@ibb.waw.pl)

  • 12-3
    •   Influence of separate genome element on cell metabolism in yeast Debaryomyces hansenii.
    Inna Sydorovych, Daria Fedorovych
    Departm.of Regulatory Systems, Institute of Cell Biology, Dragomanov 14/16, Lviv, 79005, Ukraine (inn_bohdan@yahoo.com)

  • 12-4
    •   Comparative analysis of glycerol metabolism in yeasts.
    Annamaria Merico, Ileana Vigentini, Carlo Spinelli, Bianca Maria Ranzi, Concetta Compagno
    Fisiologia e Biochimica, Università di Milano, via Celoria 26, Milano, 20133, Italy (annamaria.merico@unimi.it)

  • 12-5
    •   Role of Bat2p in Saccharomyces cerevisiae grown on non-fermentable carbon source.
    Sung Schoondermark-Stolk (1), Maria Tabernero (2), John Chapman (2), Theo Verrips (1), Arie Verkleij (1), Johannes Boonstra (1)
    (1) Molecular Cell Biology, Utrecht University, Padualaan 8, Utrecht, 3584 CH, The Netherlands (S.A.Schoondermark@bio.uu.nl); (2) Unilever Research Vlaardingen, Olivier van Noortlaan 120, 3133 AT Vlaardingen, The Netherlands

  • 12-6
    •   Ras regulates the carnitine shuttle.
    Jan H. Swiegers, Isak S. Pretorius, Florian F. Bauer
    Wine Biotechnology, Stellenbosch University, Victoria, Stellenbosch, 7600, South Africa (jhs2@sun.ac.za)

  • 12-7
    •   Transcriptome and proteome analysis of Saccharomyces cerevisiae metabolic response to glucose and ethanol as carbon sources.
    Pascale Daran-Lapujade, Mariela Serrano-Davalos, Marijke A.H. Luttik, Walter van Gulik, Jack T. Pronk
    Kluyver Lab of Biotechnology, TU Delft, Julianalaan, 67, Delft, 2628 BC, The Netherlands (p.lapujade@tnw.tudelft.nl)

  • 12-8
    •   Construction of an L-arabinose fermenting Saccharomyces cerevisiae strain by genetic engineering and evolutive screening.
    Eckhard Boles, Jessica Becker
    Institut fuer Mikrobiologie, Goethe-Universitaet Frankfurt, Marie-Curie-Str. 9, Frankfurt / Main, 60439, Germany (e.boles@em.uni-frankfurt.de)

  • 12-9
    •   Uncoupling of glucose sensitivity and deregulation of glycolysis in Saccharomyces cerevisiae tps1 mutants expressing trehalose-6-phosphate insensitive hexokinases.
    Beatriz M. Bonini, Martien De Bus, Patrick Van Dijck, Johan M. Thevelein
    Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Katholieke Universiteit Leuven and Department of Molecular Microbiology, Flemish Interuniversity Institute of Biotechnology (VIB), Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flandres, Belgium (beatriz.bonini@bio.kuleuven.ac.be)

  • 12-10
    •   TFIIB and subunits of the SAGA complex are involved in transcriptional activation of phospholipid biosynthetic genes by the regulatory protein Ino2 of the yeast Saccharomyces cerevisiae.
    Martin Dietz, Willm-Thomas Heyken, Jens Hoppen, Susanne Geburtig, Hans-Joachim Schueller
    Dept Genetics and Biochemistry, University of Greifswald, Jahnstrasse 15a, Greifswald, D-17487, Germany (schuell@biologie.uni-greifswald.de)

  • 12-11
    •   A gene of Candida albicans distantly related to OPI1 can functionally repress structural genes of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.
    Willm-Thomas Heyken, Christian Wagner, Juergen Wittmann, Antje Albrecht, Hans-Joachim Schueller
    Dept Genetics and Biochemistry, University of Greifswald, Jahnstrasse 15a, Greifswald, D-17487, Germany (heyken@uni-greifswald.de)

  • 12-12
    •   Analysis of Spsgt1, an essential orthologue of hSGT1 gene in S. pombe.
    Hiroshi Uemura (1), Tomohiro Kainou (2), Kentaro Sasaki (3), Yoji Mitsui (1), Yuko Giga-Hama (4), Hiromichi Kumagai (4)
    (1) Inst. for Biol. Resources, AIST, Higashi 1-1-1, Tsukuba-shi, 305-8566, Japan (hiroshi-uemura@aist.go.jp); (2) AIST, Japan and Asahi Glass Co., LTD., Japan; (3) Tsukuba Univ., Japan; (4) Asahi Glass Co., LTD., Japan

  • 12-13
    •   Effects of the alcohol dehydrogenases disruption on physiology and morphology in fission yeast Schizosaccharomyces pombe.
    Masao Sakurai, Hideki Tohda, Hiromichi Kumagai, Yuko Giga-Hama
    ASPEX Division, Asahi Glass Co., Ltd., 1150 Hazawa-cho, Yokohama, 221-8755, Japan (msakurai@agc.co.jp)

  • 12-14
    •   Intracellular regulation of the cysteine pool and its effect on regulation of the sulfur assimilation genes.
    Britt Bramsted (1), Jørgen Hansen (2)
    (1) Carlsberg Research Laboratory, Carlsberg Research Center, GamleCarlsbergVej 10, Copenhagen, Valby, 2500, Denmark (bb@crc.dk); (2) Biologic A/S, Thorvaldsensvej 40, 1871 Frederiksberg C

  • 12-15
    •   The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon: towards the purification and kinetic characterisation of the constituent enzymes and their complex.
    Katia Pauwels, Agnes Abadjieva, Marjolaine Crabeel
    Microbiology, Vrije Universiteit Brussel, Emile Gryson av.,1, Brussels, B-1070, Belgium (mcrabeel@vub.ac.be)

  • 12-16
    •   Duplicated genes in amino acid biosynthetic pathways have diverged towards the specialization for respiratory and fermentative growth.
    Víctor H. Anaya, Alexander DeLuna, Héctor Quezada, Alicia González
    Molecular Genetics, National University of México, Circuito Int. UNAM, Mexico City, 04510, Mexico (vanaya@ifisiol.unam.mx)

  • 12-17
    •   Genetic and biochemical characterisation of 6-phosphofructokinase from the yeast Pichia pastoris.
    Anke Edelmann, Jürgen Kirchberger, Jörg Bär
    Institute of Biochemistry, University of Leipzig, Liebigstrasse 16, Leipzig, 04103, Germany (scha@medizin.uni-leipzig.de)

  • 12-18
    •   Analysis of the One-Carbon Metabolism Transcriptional Regulon.
    Cristy L. Gelling (1), Geoffrey D. Kornfeld (2), Mathew D. W. Piper (3), Ian W. Dawes (2)
    (1) School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia; (2) Ramaciotti Centre for Gene Function Analysis and School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia (cristygelling@hotmail.com); (3) Kluyver Laboratory of Biotechnology, Technical University of Delft, Julianalaan 67, 2628BC Delft, The Netherlands

  • 12-19
    •   Cloning and stable expression of the alpha-acetolactate decarboxylase gene from Bacillus Licheniformis in yeast.
    Xiaoming Bao (1), Yujing Qin (2), Huajun Zheng (2), Aihua Hou (2), Guoliang Yang (2), Dong Gao (2)
    (1) State Key Laboratory of Microbial Technology, College of Life Sciences, Shandong University, Jinan City, 250100, P.R. China (bxm@sdu.edu.cn); (2) Shandong University, State Key Lab. of Microb Techn, Shandananlu 27#, Jinan, 250100, P. R. China

  • 12-20
    •   The fungal L-arabinose catabolic pathway.
    Peter Richard, Ritva Verho, Mikko Putkonen, John Londesborough, Merja Penttilä
    Cell Factory, VTT Biotechnology, Tietotie 2, Espoo, 02044 VTT, Finland (Peter.Richard@vtt.fi)

  • 12-21
    •   Mutations derepressing riboflavin biosynthesis suppress GTP cyclohydrolase deficiency and lead to defects in regulation of iron acquisition in the flavinogenic yeast Pichia guilliermondii.
    Kostiantyn Y. Kapustiak, Mykola M. Stenchuk, Yuriy R. Boretsky, Oleh V. Stasyk, Vasyl I. Kutsiaba, Andrei A. Sibirny
    Molecular Genetics Biotechnol., Institute of Cell Biology, Drahomanov St. 14/16, Lviv, 79005, Ukraine (sibirny@biochem.lviv.ua)

  • 12-22
    •   Buffering ATP levels in permeabilised yeast cells.
    Daniel Bosch Ibáñez, Christer Larsson, Lena Gustafsson
    Molecular Biotechnology, Chalmers University of Tech., Medicinaregatan 9c, Göteborg, 40530, Sweden (daniel.bosch@molbiotech.chalmers.se)

  • 12-23
    •   Xylose and cellobiose fermentation to ethanol by the thermotolerant methylotrophic yeast Hansenula polymorpha and by xylose fermenting yeast Pichia stipitis.
    Andrei A. Sibirny (1), Olena B. Ryabova (2), Oksana M. Chmil (2), Vladimir Sibirny (1), Zbigniew Kotylak (1), Dorota Grabek (1)
    (1) Institute of Biotechnology, Rzeszow University, Cegelniana 12, Rzeszow, 35-310, Poland (sibirny@univ.rzeszow.pl); (2) Institute of Cell Biology, Drahomanov Street 14/16, 79005, Lviv, Ukraine

  • 12-24
    •   A metabolomic analysis of yeast deletion mutants reveals complex networks of control.
    Scott J. Harrison, J. Richard Dickinson
    Cardiff School of Biosciences, Cardiff University, PO Box 915, Cardiff, CF10 3TL, United Kingdom (dickinson@cardiff.ac.uk)

  • 12-25
    •   Engineering the redox reactions of Saccharomyces cerevisiae for improved pentose fermentation.
    Ritva Verho, Peter Richard, John Londesborough, Merja Penttilä
    VTT Biotechnology, Tietotie 2, Espoo, 02044, Finland (Ritva.Verho@vtt.fi)

  • 12-26
    •   Total biosynthesis of hydrocortisone from a simple carbon source in yeast.
    Bruno Dumas (1), Denis Pompon (2), Florence, Cathy, Coralie, Amélie, Stéphane, Catherine Ménard Szczebara, Chandelier, Villeret, Masurel, Bourot, Duport (2), Agnés, Sophie, Patricia, Eric Groisillier, Blanchard, Costaglioli, Testet (3), Gilles, Eric Cauet, Degryse (4), David, Jacques Balbuena, Winter (5), Tilman Achstetter (6), Roberto Spagnoli (7)
    (1) Functional Genomics, Aventis, 13 quai J Guesdes, Vitry sur Seine, 94400, France (bruno.dumas@aventis.com); (2) Laboratoire d'Ingénierie des Protéines Membranaires, CGM du CNRS, F-91198 Gif sur Yvette, France; (3) École Supérieure de Technologie des Biomolécules de Bordeaux 2 (ESTBB), Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, F-33076 Bordeaux, France; (4) Transgene 11 Rue de Molsheim 67082 Strasbourg France; (5) Aventis, 102 Rte de Noisy 93235 Romainville; (6) Hochschule Bremen, ISTAB, Neustadtswall 30, D-28201 Bremen, Germany; (7) Lead Discovery Technologies, Aventis, 102 Rte de Noisy, 93235 Romainville

  • 12-27
    •   Regulation of the CYS3 gene of Saccharomyces cerevisiae.
    Hiroyuki Hiraishi (1), Tsuyoshi Miyake (2), Bun-ichiro Ono (1)
    (1) Biotechnology, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, 525-8577, Japan (hiraishi@se.ritsumei.ac.jp); (2) Industrial Technology Center of Okayama Prefecture, 5301 Haga, Okayama 701-1296, Japan

  • 12-28
    •   Functional characterization of the fatty acid synthase from Saccharomyces kluyveri, in vivo.
    Takahiro Oura, Susumu Kajiwara
    Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226-8501, Japan (toura@bio.titech.ac.jp)

  • 12-29
    •   Genetic engineering of a yeast strain for hyperproduction of glycerol.
    Hélène Cordier, Jean M. François
    Centre Bioing. Gilbert Durand, UMR CNRS 5504/INRA 792/INSA, 135 av. de Rangueil, Toulouse, 31077, France (helene.cordier@insa-tlse.fr)

  • 12-30
    •   A genome-wide analysis of a tps1 mutant and a molecular explanation of the stimulation of glycogen deposition in this strain.
    Jean-Luc Parrou, Marie-Ange Teste, Jean François
    Centre Bioing. Gilbert Durand, UMR CNRS5504 / INRA792/INSA, 135 ave de Rangeuil, Toulouse, 31077, France (parrou@insa-tlse.fr)

  • 12-31
    •   Cloning of structural and regulatory genes of riboflavin synthesis in the industrial flavinogenic yeast Candida famata.
    Andrei A. Sibirny (1), Kostyantyn V. Dmytruk (1), Olena P. Ishchuk (1), Andriy Y. Voronovsky (2), Charles A. Abbas (2)
    (1) Molecular Genetics Biotechnol., Institute of Cell Biology, Drahomanov St. 14/16, Lviv, 79005, Ukraine (sibirny@biochem.lviv.ua); (2) Archer Daniels Midland Company, 1001 Brush College Road, Decatur IL 62521 USA

  • 12-32
    •   Phytase production and P i-repression of the Saccharomyces cerevisiae PHO system.
    Jenny Veide, Thomas Andlid
    Chem.and Biosci./Food Science, Chalmers University of Technol, PO Box 5401, Göteborg, 40229, Sweden (jv@fsc.chalmers.se)

  • 12-33
    •   Comparative expression of novel beta-glucosidases in Saccharomyces cerevisiae.
    Ronél Van Rooyen (1), Bärbel Hahn-Hägerdal (1), Daniël C. La Grange (2), Willem H. Van Zyl (2)
    (1) Applied Microbiology, Lund University, Getingevägen 60, Lund, S-22100, Sweden (ronel.van_rooyen@tmb.lth.se); (2) Department of Microbiology, University of Stellenbosch, 7600 Stellenbosch, South Africa

  • 12-34
    •   Chimeric glucose transporters with altered transport capacity conferrespiratory glucose metabolism on Saccharomyces cerevisiae.
    Karin Otterstedt (1), Christer Larsson (2), Roslyn Bill (2), Eckhard Boles (3), Stefan Hohmann (4), Lena Gustafsson (2)
    (1) Department of Molecular Biotechnology, Chalmers University of Technology, Box462, S-405 30 Göteborg, Sweden (Karin.Otterstedt@molbiotech.chalmers.se); (2) Molecular Biotechnology, Chalmers, BOX, Göteborg, 405 30, Sweden; (3) Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätsstrasse1, Geb. 26.12.01, D-40225 Düsseldorf, Germany; (4) Department of Cell and Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden

  • 12-35
    •   Physiological role of the second alcohol oxidase gene MOD2 in the methylotrophic growth of Pichia methanolica.
    Tomoyuki Nakagawa (1), Yasuyoshi Sakai (2), Hiroyuki Mukaiyama (2), Tatsuro Miyaji (1), Hiroya Yurimoto (2), Nobuo Kato (2), Noboru Tomizuka (1)
    (1) Dept. of food sci. and tech., Tokyo univ. of agri., Yasaka 196, Abashiri, 099-2493, Japan (t-nakaga@bioindustry.nodai.ac.jp); (2) Div. of Appl Life Sci., Kyoto Univ., Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan

  • 12-36
    •   Methylotrophic pathway particitates in pectin utilization by Candida boidinii.
    Shuki Fujimura (1), Tomoyuki Nakagawa (1), Tatsuro Miyaji (1), Hiroya Yurimoto (2), Yasuyoshi Sakai (2), Nobuo Kato (2), Noboru Tomizuka (1)
    (1) Dept. of Food Sci. and Tech., Tokyo Univ. of Agri., Yasaka 196, Abashiri, 099-2493, Japan (t-nakaga@bioindustry.nodai.ac.jp); (2) Div. of Appl Life Sci., Kyoto Univ., Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan

  • 12-37
    •   Characterisation of KlCat8p regulation in Kluyveromyces lactis.
    Godefroid Charbon (1), Isabelle Georis (2), Monique Dewez (1), Karin Breunig (3), Jean Vandenhaute (1)
    (1) Laboratoire de Génétique Moléculaire - F.U.N.D.P. U.R.B.M. - 61, rue de Bruxelles - B-5000 Namur Belgium (godefroid.charbon@fundp.ac.be); (2) Laboratoire de Chimie Biologique - U.M.H - 6, Avenue du Champ de Mars - 7000 Mons Belgium; (3) Institut für Genetik - Martin-Luther Universität Halle-Wittenberg -Domplatz, 1 - D-06099 Halle (Saale) Germany

  • 12-38
    •   Pathways for acetate formation in S. cerevisiae during glucose fermentation : role of the five ALD genes.
    Florence Saint-Prix, Sylvie Dequin
    UMR SPO - Microbiologie, INRA, 2 place Viala, Montpellier, 34060, France (stprix@ensam.inra.fr)

  • 12-39
    •   Deletion of the redox regulated GPD2 gene in Saccharomyces cerevisiae strongly alters Tdh1p expression during anaerobic growth.
    Äsa Valadi, Hadi Valadi, Ricky Ansell, Lena Gustafsson, Joakim Norbeck, Lennart Adler, Anders Blomberg
    CMB-microbiology, Göteborg University, Medicinaregatan 9C, Göteborg, 41390, Sweden (asa.valadi@gmm.gu.se)

  • 12-40
    •   Engineering of Saccharomyces cerevisiae for production of L-glycerol 3-phosphate.
    Huyen Thi Thanh Nguyen (1), Nam Hai Truong (2), Ulf Stahl (1), Elke Nevoigt (1)
    (1) Microbiology and Genetics, Berlin University of Technolog, Seestrasse 12, Berlin, 13353, Germany (E.Nevoigt@LB.TU-Berlin.de); (2) Vietnam National Centre for Natural Science and Technology, Institute of Biotechnology, Hoang Quoc Viet-Cau Giay, Hanoi, Vietnam

  • 12-41
    •   Metabolic uncoupling and glycolytic flux regulation in Saccharomyces cerevisiae.
    Elisabeth Thomsson, Lena Gustafsson, Christer Larsson
    Molecular Biotechnology, Chalmers Univ. of Technology, Medicinaregatan 9E, Göteborg, 413 90, Sweden (elisabeth.thomsson@molbiotech.chalmers.se)

  • 12-42
    •   Evolutionary engineering of Saccharomyces cerevisiae for anaerobic growth on xylose.
    Marco Sonderegger, Uwe Sauer
    Institute of Biotechnology, ETH Zurich, ETH Hoenggerberg, HPT, Zurich, 8093, Switzerland (sonderegger@biotech.biol.ethz.ch)

  • 12-43
    •   Protein Engineering for the Improvement of Enzymatic Activity: a site-specific Mutagenesis of a Lactate Dehydrogenase (LDH) expressed in Saccharomyces cerevisiae.
    Paola Branduardi, Luca De Gioia, Giuseppe Zampella, Piercarlo Fantucci, Lilia Alberghina, Danilo Porro
    Dip. Biotecnol. e Bioscienze, Università Milano-Bicocca, P.zza della Scienza, Milano, 20126, Italy (danilo.porro@unimib.it)

  • 12-44
    •   Improving stereo-selective whole cell bioreduction with recombinant Saccharomyces cerevisiae.
    Mikael Katz, Ted Johanson, Bärbel Hahn-Hägerdal, Marie Francois Gorwa Grauslund
    Applied Microbiology, Lund institute of technology, Getingevägen 60, Lund, 221 00, Sweden (Ted.Johanson@tmb.lth.se)

  • 12-45
    •   Mevalonate pathway intermediate detection in Saccharomyces cerevisiae via LC-MS and GC-MS.
    Tamay Seker, Kasper Moller, Jens Nielsen
    Center for Process Biotech., BioCentrum-DTU, Building 223, Lyngby, DK-2800, Denmark (tseker@biocentrum.dtu.dk)

  • 12-46
    •   Genetical engineering of the carotenoid biosynthetic pathway in Xanthophyllomyces dendrorhous.
    Hans Visser (1), Jan Verdoes (2), Gerhard Sandmann (3), Johan van den Berg (1)
    (1) Fungal Genomics, Wageningen University, Dreijenlaan 2, Wageningen, 6703 HA, The Netherlands (hans.visser@wur.nl); (2) GenoClipp Biotechnology BV, L.J. Zielstraweg 1, 9713 GX, Groningen, The Netherlands; (3) Botanical Institute, J.W. Goethe University, PO Box 111932, 60054 Frankfurt am Main, Germany

  • 12-47
    •   Isolation and analysis of constitutive invertase mutants resistant to high levels of glucose from Schizosaccharomyces pombe.
    Güler Temizkan (1), Cenk Kıg (1), Sezai Türkel (2)
    (1) Istanbul University, Department of Biology, Vezneciler, Box 34459, Istanbul, Turkey (temizkan@istanbul.edu.tr); (2) Uludag University, Department of Biology, Bursa, Turkey

  • 12-48
    •   Physiological effects of deleting the gene YPL201c in a glycerol overproducing strain of Saccharomyces cerevisiae during anaerobic growth.
    Tobias Modig (1), Katarina Granath (2), Lennart Adler (2), Gunnar Lidén (1)
    (1) Chemical Engineering, Lund University, Box 124, Lund, 22100, Sweden (tobias.modig@chemeng.lth.se); (2) Department of Cell and Molecular Biology, Göteborg University, Box 462, 405 30 Göteborg, Sweden

  • 12-49
    •   Construction of lactic acid producing sour dough yeast Torulaspora delbrueckii.
    Matti Korhola (1), Marja Aittamaa (1), Richard Degré (2), Hilkka Turakainen (1)
    (1) Biosciences, University of Helsinki, Viikinkaari 9, Helsinki, FIN-00014, Finland (matti.korhola@helsinki.fi); (2) Lallemand Inc., 1620 Prefontaine, Montreal, Quebec, H1W 2N8 Canada

  • 12-50
    •   Use of metabolic engineering to enhance xylose consumption rate and ethanol yield in recombinant Saccharomyces cerevisiae.
    Marie Jeppsson, Björn Johansson, Karin Träff, Bärbel Hahn-Hägerdal, Marie F. Gorwa-Grauslund
    Applied Microbiology, Lund Institute of Technology, Getingevägen 60, Lund, 221 00, Sweden (Barbel.Hahn-Hagerdal@tmb.lth.se)

  • 12-51
    •   Control of dynamic cell(-cell) signalling.
    Sergio Rossell (1), Barbara M. Bakker (1), Jacky L. Snoep (2), Karin Reijenga (3), Jasper Diderich (4), Hans V. Westerhoff (1)
    (1) Molecular Cell Physiology, CRBCS, FALW, VU, De Boelelaan 1087, Amsterdam, NL-1081 HV, The Netherlands, EU (hw@bio.vu.nl); (2) Stellenbosch University, South Africa; (3) DSM, Delft, NL. EU; (4) Technical University, Delft, NL, EU

  • 12-52
    •   Isolation of a yeast strain that overproduces threonine in a temperature-dependent manner. An aspartate kinase with thermosensitive regulation?
    Isabel Velasco (1), Miguel Arevalo-Rodriguez (2), Pablo Marina (1), Isabel L. Calderon (1)
    (1) Department of Genetics, University of Seville, Ap. 1095, Seville, E-41080, Spain (ilcalderon@us.es); (2) Present Address: The Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA

  • 12-53
    •   Participation of an nitric oxide synthase in the oxygen dependent regulation of fermentation in Pichia stipitis.
    Ulrich Klinner (1), Magnus Mergler (1), Volkmar Passoth (2)
    (1) Institut für Biologie IV, RWTH Aachen, Worringer Weg, Aachen, 52056, Germany; (2) Swedish University of Agricultural Sciences (SLU), Dept. of Microbiology, Uppsala Genetic Centre, Box 7025, S-750 07 Uppsala, Sweden (ulrich.klinner@rwth-aachen.de)

    Poster Session 13 - Diversity, regulation and signalling function of membrane transporters

  • 13-1
    •   Functional expression of the Oryza sativa NHX1 antiporter in Saccharomyces cerevisiae.
    Olga Kinclova, Hana Flegelova, Hana Sychrova
    Dept. Membrane Transport, Institute of Physiology, Videnska 1083, Prague 4 - Krc, 142 20, Czech Republic (kinclova@biomed.cas.cz)

  • 13-2
    •   Characterization of S. cerevisiae mutants lacking the KHA1 gene.
    Lydie Maresova, Hana Sychrova
    Dept. Membrane Transport, Inst. Physiology, CzAcadSci, Videnska 1083, Prague 4, 142 20, Czech Republic (lydie@biomed.cas.cz)

  • 13-3
    •   Translocation of the C-terminus of a tail-anchored protein across the ER membrane in yeast mutants defective in signal peptide-driven translocation.
    Monica Yabal (1), Nica Borgese (2), Marja Makarow (1)
    (1) Prog.of Cellular Biotechnology, Institute of Biotechnology, Viikinkaari 9, Helsinki, 00014, Finland (monica.yabal@helsinki.fi); (2) Consiglio Nazionale delle Ricerche Institute of Neuroscience, Cellular and Molecular Pharmacology Section, Via Vanvitelli 32 - 20129 Milano, Italy

  • 13-4
    •   Alteration of Saccharomyces cerevisiae plasma membrane fluidity is accompanied with the reduced sensitivity of cells to the killer toxin K1.
    Drahomíra Novotná (1), Hana Flegelová (1), Roman Chaloupka (2), Jan Malác (2), Dana Gásková (2), Blanka Janderová (1), Karel Sigler (3)
    (1) Dept. Genet. Microbiol., Fac. Sci., Charles University, Vinicná 5, Prague 2, 128 44, Czech Republic (dranov@natur.cuni.cz); (2) Institute of Physics, Charles University, Ke Karlovu 5, 121 16 Prague 2, Czech Republic; (3) Institute of Microbiology, Czech Academy of Sciences, Videnská 1083, 142 20 Prague 4, Czech Republic

  • 13-5
    •   The functioning of rat CLC-2 chloride channel in Saccharomyces cerevisiae cells requires an increased level of the Kha1 protein.
    Krzysztof Flis , Anna Kurlandzka
    Department of Genetics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland (kflis@bp.onet.pl)

  • 13-6
    •   Structure-function analysis of the major proline transporter of Aspergillus nidulans (PrnB) using Cys-scanning mutagenesis.
    Panagiota Kafasla (1), Stathis Frillingos (2), Vicky Sophianopoulou (1)
    Institute of Biology, National Center for Scientific Research Demokritos (NCSRD), Aghia Paraskevi 153 10, Athens, Greece (ykafasla@bio.demokritos.gr); (2) Laboratory of Biological Chemistry, University of Ioannina, Medical School, Ioannina 451 10, Greece

  • 13-7
    •   New putative ammonium exporters Ato1p, Ato2p and Ato3p in Saccharomyces cerevisiae colonies.
    Marketa Ricicova, Zdena Palkova
    Genetics and Microbiology, Charles University, Vinicna 5, Prague, 12844, Czech Republic (marici@email.cz)

  • 13-8
    •   The role of the Ymr212c/Efr3 membrane protein on the function of human glucose transporters in yeast.
    Doerthe Voss, Roman Wieczorke, Eckhard Boles
    Institut fuer Mikrobiologie, J.W.-Goethe-Universitaet, Marie-Curie-Str. 9, Frankfurt/Main, 60439, Germany (Voss@em.uni-frankfurt.de)

  • 13-9
    •   Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces cerevisiae.
    Youngwoo Kim (1), Cheol-Won Yun (2), Robert E. Moore (3), Caroline C. Philpott (4)
    (1) Liver Diseases Section, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA; (2) Korea Research Institute of Chemical Technology, Yusong, Taejon, 305-600, Korea; (3) Department of Chemistry, University of Wisconsin, 800 Algoma Blvd. Oshkosh, WI, USA; (4) Liver Diseases Section, NIDDK, National Institutes of Health, Bethesda, MD, USA (carolinep@intra.niddk.nih.gov)

  • 13-10
    •   A role of P-type Cta4 ATPase and calcineurin in calcium homeostasis and endoplasmic reticulum stress in fission yeast.
    Ana Cristina Lustoza (1), Karl Ekwall (2), Lev Okorokov (1), Anna Okorokova-Facanha (1)
    (1) LFBM/CBB, UENF, Alberto Lamego 2000, Campos Goytacazes, 28015-620, Brazil (anna@uenf.br); (2) Karolinska Institutet, Dept. of Biosciences Novum, University College Sodertorn, Dept. of Natural Sciences, S-141 89, Huddinge, Sweden

  • 13-11
    •   Effect of lipids on trafficking of proteins within the plasma membranes of yeast.
    Miroslava Opekarová (1), Katerina Malínská (2), Jan Malínsky (3), Widmar Tanner (2)
    (1) Cell Biology, Institute of Microbiology, Vídenská 1083, Prague, 142 20, Czech Republic (opekaro@biomed.cas.cz); (2) Universität Regensburg, Lehrstuhl für Zellbiologie und Pflanzenphysiologie, 93040 Regensburg, Germany; (3) Institute of Medicine CAS and 1st Faculty of Medicine, Charles University, Albertov 4, 12801 Prague 2, Czech Republic

  • 13-12
    •   Subcellular localisation of an active green fluorescent protein-tagged GUP1 glycerol transporter in Saccharomyces cerevisiae.
    Gianluca Bleve (1), Francesco Grieco (2), Maria Stella Cappello (2), Concetta Santonocito (2), Franco Dellaglio (1), Giuseppe Zacheo (2)
    (1) Dip. Scientifico e Tecnologico, Università di Verona, Strada Le Grazie 15, Verona, 37134, Italy (bleve@sci.univr.it); (2) Istituto di Scienze delle Produzioni Alimentari del CNR, Sezione di Lecce, Via Prov. Lecce-Monteroni, 73100 Lecce Italy

  • 13-13
    •   Expression of mammalian Na + /H + antiporters in yeast.
    Hana Flegelova, Olga Kinclova, Hana Sychrova
    Membrane Transport, Inst. Physiology CzAcadSci, Videnska 1083, Prague 4 , 142 20, Czech Republic (flegel@biomed.cas.cz)

  • 13-14
    •   The AQR1 transporter mediates amino acid excretion in Saccharomyces cerevisiae.
    Isabel Velasco (1), Isabel L. Calderón (1), Bruno André (2)
    (1) Departamento de Genética, Universidad de Sevilla, Av.Reina Mercedes, 6, Sevilla, 41012, Spain (umpier@us.es); (2) Laboratoire de Physiologie Cellulaire. Institut de Biologie et de Médecine Moléculaires. Université Libre de Bruxelles. Rue des Pr. Jeener et Brachet, 12. 6041 Gosselies. Belgium.

  • 13-15
    •   Molecular and physiological characterization of monocarboxylic acids permeases in the yeast Kluyveromyces lactis.
    Odília Queirós (1), Sandra Paiva (2), Pedro Moradas-Ferreira (3), Margarida Casal (2)
    (1) IBMC, Univ. Porto, Porto, CCA, Dep. Biologia, Univ. Minho, ISCSN, 4580 Paredes (oqueiros@ibmc.up.pt); (2) CCA, Dep. Biologia, Univ. Minho; (3) IBMC, Univ. Porto, Porto, ICBAS, Univ. Porto, 4100 Porto

  • 13-16
    •   The role of transportproteins for the heavy metal resistance of Schizosaccharomyces pombe.
    Silke Hammelmann, Klaus Wolf, Martin Zimmermann
    Biology IV, RWTH-Aachen, Worringerweg, Aachen, 52056, Germany (silkehammelmann@hotmail.com)

  • 13-17
    •   Investigation of hexose transporter interaction.
    Corinna Cappellaro, Eckhard Boles
    Mikrobiologie, Universitaet Frankfurt, Marie-Curie-Sr. 9, Frankfurt, 60439, Germany (C.Cappellaro@em.uni-frankfurt.de)

  • 13-18
    •   A molecular model describing the interactions of a purine transporter with different substrates.
    Marina Koukaki, George Diallinas
    Faculty of Biology, University of Athens, Panepistimioupolis, Athens, 15781, Greece (diallina@biol.uoa.gr)

  • 13-19
    •   Development of a model system to study the topogenesis of the nucleobase-ascorbate transporter family.
    Areti Pantazopoulou, Marina Koukaki, Anna Vlanti, George Diallinas
    Faculty of Biology, University of Athens, Panepistimioupolis, Athens, 15781, Greece (diallina@biol.uoa.gr)

  • 13-20
    •   Receptor-mediated endocytosis, retrograde transport and ER dislocation of the yeast K28 virus toxin.
    Susanne Leis, Katrin Eisfeld, Manfred J. Schmitt
    University of the Saarland, Applied Molecular Biology, Building 2, Saarbrücken, D-66041, Germany (sl@microbiol.uni-sb.de)

  • 13-21
    •   Maltotriose transport in yeast.
    Annel Smit (1), Isak S Pretorius (2), Ricardo R Cordero Otero (1)
    (1) Viticulture and Oenology, Inst. for Wine Biotechnology, Victoria street, Stellenbosch, 7600, South Africa (iwbt37@sun.ac.za); (2) The Australian Wine Research Institute, Adelaide, Australia

  • 13-22
    •   Potassium transport systems in Debaryomyces hansenii.
    Catarina Prista (1), José Ramos (2), Maria C. Loureiro-Dias (1)
    (1) DBEB, ISA-UTL, Calç. da Tapada, Lisboa, 1349-017, Portugal (cprista@isa.utl.pt); (2) Departamento de Microbiología Ed. Severo Ochoa, Campus Rabanales, Córdoba, España

  • 13-23
    •   Biochemical and functional analysis of the role of Ctr2p in copper transport in Saccharomyces cerevisiae.
    Erin M. Rees, Jaekwon Lee, Dennis J. Thiele
    Biological Chemistry, University of Michigan, 1301 Catherine, Ann Arbor, MI 48109, USA (erees@umich.edu)

  • 13-24
    •   Roles of low affinity hexose transporters in Saccharomyces cerevisiae under wine conditions.
    Christine Riou (1), Kattie Luyten (2), Sylvie Dequin (1), Bruno Blondin (1)
    (1) UMR-Sciences pour l'Oenologie, ENSAM-INRA, Bat. 28, 2, place Viala, Montpellier, F-34060, France (riou@ensam.inra.fr); (2) SYNPORE GmbH, Auf der Morgenstelle I, D-72076 Tübingen, Germany

  • 13-25
    •   Distinct amino acid residues within the pore loop determine K+-dependent gating and pore stability in the yeast K+ channel, Tok1.
    Gabriel Natura (1), Anja Roller (2), Hermann Bihler (2), Adam Bertl (2)
    (1) Institut für Physiologie I, Universität Jena, 07743 Jena, Germany; (2) Botanik I, Universität Karlsruhe, Kaiserstrasse 2, Karlsruhe, 76128, Germany (adam.bertl@chemie-bio.uni-karlsruhe.de)

  • 13-26
    •   Electrophysiological analysis of the yeast V-ATPase: delta pH-dependent coupling ratio and modulation by cytosolic ADP.
    Carsten Kettner (1), Gerhard Obermeyer (2), Adam Bertl (3), Hermann Bihler (3)
    (1) Beilstein-Institut zur Förderung der chemischen Wissenschaften, D-60487 Frankfurt, Germany; (2) Institut für Pflanzenphysiologie, Universität Salzburg, A-5020 Salzburg, Austria; (3) Botany I, University of Karlsruhe, Kaiserstrasse 2, Karlsruhe, 76131, Germany (db49@rz.uni-karlsruhe.de)

  • 13-27
    •   The first known gene encoding a plasma-membrane transporter for vitamin B-6.
    Juergen Stolz, Martin Vielreicher
    Dept. of Cell Biology, Regensburg University, Universitaetsstr. 31, Regensburg, D-93040, Germany (juergen.stolz@biologie.uni-regensburg.de)

  • 13-28
    •   The disruption of JEN1 from Candida albicans impairs the transport of lactate.
    Sandra Paiva (1), Isabel Soares-Silva (1), Peter Kötter (2), Karl-Dieter Entian (2), Margarida Casal (1)
    (1) Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal (spaiva@bio.uminho.pt); (2) Institut für Mikrobiologie, Johann Wolfgang Goethe-Universität, Marie-Curie-Strasse 9, 60439 Frankfurt/Main, Germany

  • 13-29
    •   Utilization of S. cerevisiae mutants defective in high affinity copper uptake to characterize copper transporters from plants and mammals.
    Sergi Puig (1), Vicente Sancenon (2), Lola Peñarrubia (2), Dennis J. Thiele (1)
    (1) Dept. of Biological Chemistry, University of Michigan Med. Sch, 1301 Catherine Rd, Ann Arbor, MI 48109-0606, USA (spuig@umich.edu); (2) Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, Dr. Moliner 50, Burjassot, Valencia, 46100 Spain.

  • 13-30
    •   Molecular characterization of the fructose transporter of Zygosaccharomyces bailii.
    Carlos Pina (1), Paula Gonçalves (2), Catarina Prista (1), Maria C. Loureiro-Dias (1)
    (1) DBEB, ISA-UTL, Calç. da Tapada, Lisboa, 1349-017, Portugal (mcdias@isa.utl.pt); (2) Centro de Recursos Microbiológicos, Secção Autónoma de Biotecnologia, FCT/UNL, 2825-516 Caparica, Portugal

  • 13-31
    •   FSY1, the gene encoding a specific fructose/H + carrier in Saccharomyces carlsbergensis, is differentially repressed by fructose and glucose.
    Helena Rodrigues de Sousa, Isabel Spencer-Martins, Paula Gonçalves
    CREM/SABT, FCT/UNL, Quinta da Torre, Caparica, 2828-516, Portugal (pmz@mail.fct.unl.pt)

  • 13-32
    •   Analysis of phosphate uptake kinetics in chemostat culture of Saccharomyces cerevisiae.
    Soheila Shokrollahzadeh (1), Babak Bonakdarpour (2), Farzaneh Vahabzadeh (2), Mehri Sanati (3), Bengt L. Persson (4)
    (1) Department of Chemical Engineering, Amirkabir University of Technology, Tehran-15914, Iran; School of Biosciences and Process Technology, Växjö University, SE-35195 Växjö, Sweden; Iranian Research Organization for Science and Technology (IROST), P.O. Box 15815-3538, Tehran-15819, Iran (soheila.shokrollahzadeh@ibp.vxu.se); (2) Department of Chemical Engineering, Amirkabir University of Technology, Tehran-15914, Iran; (3) School of Biosciences and Process Technology, Växjö University, SE-35195 Växjö, Sweden; (4) Department of Biochemistry and Biophysics, Wallenberg Labortory, Stockholm University, Sweden; Department of Chemistry and Biomedical Sciences, Kalmar University, 39129 Kalmar, Sweden (Bengt.Persson@hik.se)

  • 13-33
    •   Characterization of the human PiT, phosphate transport protein, using the yeast model organism Saccaromyces cerevisiae.
    Fredrik Lundh (1), Bengt L. Persson (2)
    (1) Department of Chemistry and Biomedical Science, Kalmar University, S-391 29 Kalmar (fredrik.lundh@hik.se); (2) Department of Chemistry and Biomedical Science, Kalmar University, S-391 29 Kalmar, and Department of Biochemistry and Biophysics, Stockholm University, S-106 91 Stockholm, Sweden

  • 13-34
    •   Characterisation of the Saccharomyces aquaporins.
    Frederic Sidoux-Walter (1), Vincent Laize (2), Nina Pettersson (1), Stefan Hohmann (1)
    (1) Department of Cell and Molecular Biology - Microbiology, Göteborg University, Box 462, SE-405 30 Göteborg, Sweden (frederic.sidoux-walter@gmm.gu.se); (2) University of Algarve, UCTRA - Campus de Gambelas, 8000-810 Faro, Portugal

  • 13-35
    •   Using yeast to study structure and function of mammalian aquaglyceroporins.
    Nina Pettersson, Stefan Hohmann
    Department of Cell and Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden, (nina.pettersson@gmm.gu.se)

  • 13-36
    •   Molecular characterisation of xylose transport in Candida intermedia.
    Maria J. Leandro, Paula Gonçalves, Isabel Spencer-Martins
    CREM, Biotechnology Unit, FCT/UNL, Quinta da Torre, Caparica, 2829 -516, Portugal (maria_leandro@hotmail.com)

  • 13-37
    •   Exploring genetic tools for the over-expression of the lactate permease Jen1p of Saccharomyces cerevisiae : constitutive expression in S. cerevisiae and heterologous expression in Pichia pastoris.
    Isabel Soares-Silva, Raquel P. Andrade, Dorit Schuller, Margarida Casal
    Centro de Ciências do Ambiente, Departamento de Biologia, Universidade do Minho, 4710-057 Braga, Portugal (ijoao@bio.uminho.pt)

  • 13-38
    •   Analysis of the control of the Fps1p glycerol efflux channel.
    Caroline Filipsson (1), Kristina Hedfalk (2), Jan Rydström (1), Stefan Hohmann (3), Roslyn Bill (4)
    (1) Chemistry/Biochemistry, Göteborg University, Box 462, Göteborg, 403 30, Sweden (caroline.filipsson@bcbp.gu.se); (2) Department of Chemistry and Bioscience/Molecular Biotechnology, Chalmers University of Technology, S-405 30 Göteborg, Sweden; (3) Department of Cell and Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden; (4) School of Life and Health Science, Aston University, Aston Triangle, Birmingham B4 7ET, United Kingdom

  • 13-39
    •   Polyol transport in yeast: studying transport proteins and manipulating osmotic responses.
    Sara Karlgren (1), Roslyn Bill (2), Stefan Hohmann (1)
    (1) Cell and Molecular Biology, Microbiology, Box 462, Göteborg, 405 30, Sweden (sara.karlgren@gmm.gu.se); (2) School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK

  • 13-40
    •   Functional reconstitution, regulation of expression, and targeting of Ant1p, the peroxisomal adenine nucleotide carrier.
    Hanspeter Rottensteiner (1), Luigi Palmieri (2), Ferdinando Palmieri (2), Aner Gurvitz (3), Ralf Erdmann (1)
    (1) FU Berlin, Institute of Biochemistry, Thielallee 63, Berlin, 14195, Germany (hpr@zedat.fu-berlin.de); (2) University of Bari, Department of Pharmaco-Biology, Via E. Orabona 4, 70125 Bari, Italy; (3) University of Vienna, Institute of Biochemistry and Molecular Cell Biology, Dr. Bohrgasse 9, 1030 Wien, Austria

  • 13-41
    •   Saccharomyces cerevisiae glycerol uptake: new insights.
    Luisa Neves (1), Fernanda Lages (1), Rui Oliveira (1), Anders Brandt (2), Morten Kielland-Brandt (2), Candida Lucas (1)
    (1) Biology, Minho University, Campus Gualtar, Braga, 4710-057, Portugal (luisavieiraneves@hotmail.com); (2) Department of Physiology, Carlsberg Laboratory, Gamle Carlsberg VEJ 10, DK-2500 Copehagen Valby, Denmark

  • 13-42
    •   Saccharomyces cerevisiae GUP1, a gene involved in active glycerol uptake: new phenotypes.
    Célia Ferreira (1), Cândida Lucas (1), Anders Brandt (2), Morten Kielland-Brandt (2)
    (1) Biologia, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal (celiaf@crc.dk); (2) Department of Physiology, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Copenhagen Valby, Denmark

  • 13-43
    •   Involvement of SUT1 in hypoxic-induced sterol uptake in Saccharomyces cerevisiae.
    Parissa Alimardani (1), Thierry Berges (2)
    (1) Laboratory of Yeast Genetics, 40 Avenue Recteur PINEAU, BAT GON, 86022 POITIERS (marina.alimardani@etu.univ-poitiers.fr); (2) Laboratory of Yeast Genetics, Univers of Sciences, 40 Recteur PINEAU, POITIERS, 86000, France

  • 13-44
    •   Fps1p channel is the mediator of the major part of glycerol passive diffusion in Saccharomyces cerevisiae : artefacts and re-definitions.
    Rui Oliveira, Fernanda Lages, Magda Silva-Graça, Cândida Lucas
    Departamento de Biologia, Universidade do Minho, Campus de Gualtar, Braga, 4710-057, Portugal (ruipso@bio.uminho.pt)

  • 13-45
    •   Characterisation of KlGcr1p and KlGcr2p of Kluyveromyces lactis, two transcription factors required for glycolytic gene expression and the RAG1 glucose permease gene regulation.
    Helen Neil, Marc Lemaire, Micheline Wésolowski-Louvel
    UMR5122 Microbiology & Genetic, UCB Lyon I, 10, rue Dubois, Villeurbanne, 69622, FRANCE (mlemaire@univ-lyon1.fr)

  • 13-46
    •   Amino-acid signalling in yeast : overproduction of Ssy5 factor leads to constitutive activation of AGP1 gene transcription via an upstream UASAA element.
    Fadi Abdel Sater (1), Bruno André (1)
    (1) IBMM, ULB, 12, Rue des Prf Jeener et Brachet 6041 Gosselies Belgium (fadelsa@ulb.ac.be)

  • 13-47
    •   The transcriptional regulation of the RAG1 glucose permease gene of Kluyveromyces lactis requires the enolase gene and a functional glycolytic flux.
    Marc Lemaire, Micheline Wésolowski-Louvel
    UMR5122 Microbiology & Genetic, UCB Lyon I, 10, rue Dubois, Villeurbanne, 69622, FRANCE (mlemaire@univ-lyon1.fr)

  • 13-48
    •   Cloning and characterization of a high-affinity maltose transporter from Torulaspora delbrueckii.
    Cecília Araéjo (1), Maria Jose Hernandez-Lopez (2), Maria Judite Almeida (1), Francisca Randez-Gil (2), Maria João Sousa (1)
    (1) Department of Biology, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal (cecilia.araujo@oninet.pt); (2) Department of Biotecnology. Instituto de Agroquímica y Tecnología de los Alimentos. Consejo Superior de Investigaciones Científicas, PO Box 73, 46100 Burgassot. Valencia, Spain

  • 13-49
    •   Structure, function and interaction of the Pho84 and Gtr1 proteins involved in phosphate uptake in Saccharomyces cerevisiae.
    Jens O. Lagerstedt (1), Kent I. Lundh (2), Ian Reeve (3), John C. Voss (3), Bengt L. Persson (1)
    (1) Department of Chemistry and Biomedical Science, Kalmar University, Box 905, S-391 29 Kalmar; and Department of Biochemistry and Biophysics, Stockholm University, S-106 91 Stockholm, Sweden (jens.lagerstedt@hik.se); (2) Department of Chemistry and Biomedical Science, Kalmar University, Box 905, S-391 29 Kalmar, Sweden; (3) Department of Biological Chemistry, University of California Davis, Davis, CA 95616 USA

  • 13-50
    •   Identification of two phosphate transporters in the yeast Yarrowia lipolytica.
    Johanna Pattison-Granberg (1), Renata Zwyagilskaya (2), Bengt Persson (3)
    (1) Department of Chemistry and Biomedical sciences, Kalmar University, 391 29 Kalmar, Department of Biochemistry and Biophysics, Stockholm University, 106 91 Stockholm, Sweden, Sweden (johanna.granberg@ibp.vxu.se); (2) Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia; (3) Department of Chemistry and Biomedical sciences, Kalmar University, 391 29 Kalmar, Sweden, Department of Biochemistry and Biophysics, Stockholm University, 106 91 Stockholm, Sweden

  • 13-51
    •   Functional and structural analysis of the hexose transporter chimera TM6.
    Cecilia Henricsson, Euan Gordon, Lena Gustafsson
    Molecular Biotechnology, Molecular Biotechnology, Box462, Gothenburg, 40530, Sweden (cecilia.henricsson@molbiotech.chalmers.se)

  • 13-52
    •   Magnesium uptake and homeostasis in yeast.
    Richard Gardner (1), Jo Hanley (1), Keith Richards (1), Salam Salih (1), Paul Donaldson (2), Van Kelly (1), Kate Thodey (1)
    (1) School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand (r.gardner@auckland.ac.nz); (2) School of Medicine, University of Auckland, Private Bag 92019, Auckland, New Zealand

  • 13-53
    •   A short regulatory domain restricts glycerol transport through yeast Fps1p.
    Markus J. Tamás (1), Sara Karlgren (1), Roslyn M. Bill (2), Johan M. Thevelein (3), Jan Rydström (4), Jonathan G.L. Mullins (5), Stefan Hohmann (1)
    (1) Cell and Molecular Biology, Göteborg University, Box 462, Göteborg, 405 30, Sweden (markus.tamas@gmm.gu.se); (2) School of Life and Health Sciences, Aston University, UK; (3) Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Belgium; (4) Department of Biochemistry and Biophysics, Göteborg University, Sweden; (5) Faculty of Health and Social Sciences, University of Luton, UK

  • 13-54
    •   Visualization of protein compartmentation within the plasma membrane of living yeast cells.
    Katerina Malinska (1), Jan Malinsky (2), Miroslava Opekarova (3), Widmar Tanner (1)
    (1) Lehrstuhl für Zellbiologie, Universität Regensbrg, Universitätstr. 31, Regensburg, 930 40, Germany (katerina.malinska@biologie.uni-regensburg.de); (2) Institute of Medicine, CAS, and 1st Faculty of Medicine, Charles University, Albertov 4, 12801 Prague 2, Czech Republic; (3) Institute of Microbiology, CAS, 14220 Prague 4, Czech Republic

  • 13-55
    •   Functional consequences of the in-frame insertion of a transposon into the mutated gamma amino butyric acid transporter of Saccharomyces cerevisiae.
    Fakhraddin Naghibalhossaini (1), Francine Nault (2), Uri Saragovi (3), Haynu Nedev (3), Rose M. Johnstone (2)
    (1) Biochemistry, Shiraz University of Medical Sciences, Zand Street, Shiraz, 71345-1167, Iran (fakhraddin.naghibalhossaini@elf.mcgill.ca); (2) Department of Biochemistry, McGill University, Montreal, Canada; (3) Department of Pharmacology and Therapeutics, McGill University, Montreal, Canada

  • 13-56
    •   Functional hyper-expression of membrane proteins in yeast and discovery of chemosensitizers.
    Brian Monk (1), Kyoko Niimi (1), Erwin Lamping (1), Ann Holmes (1), Richard Cannon (1), David RK Harding (2), Andre Goffeau (3), Anabelle Decottignies (3), Shun-ici Wada (4), Masakazu Niimi (4), Yoshimasa Uehara (4)
    (1) Department of Oral Sciences, University of Otago, Dunedin, New Zealand; (2) Centre for Separation Science, Institute for Fundamental Sciences, Massey University, New Zealand; (3) FYSA Unit, UCL, Croix du Sud, 2/20, Louvain-la-Neuve, 1348, Louvain-la-Neuve, Belgium (goffeau@fysa.ucl.ac.be); (4) Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo, Japan

    Poster Session 14 - Control of cell growth and cell division

  • 14-1
    •   Co-ordination of spindle orientation and mitotic progression in fission yeast.
    Sylvie Tournier (1), Yannick Gachet (2), Jeremy Hyams (2), Jonathan Millar (1)
    (1) Division of Yeast Genetics, Nat Inst for Medical Research, Mill Hill, London, NW7 1AA, England (jmillar@nimr.mrc.ac.uk); (2) Department of Biology, University College London, Gower Street, London

  • 14-2
    •   HAT Gcn5p is required for proper chromosome segregation and mitotic exit.
    Patrizia Filetici (1), Enrico Cundari (1), Emanuela De Cinti (2), Roberta Morisi (2), Prisca Ornaghi (2), Enzo Marchetti (2), Paola Ballario (2)
    (1) IBPM, CNR, c/o Dept. Genetica e Biologia Molecolare, Università La Sapienza, P.le A.Moro 5, 00185 Roma-Italy (patrizia.filetici@uniroma1.it); (2) Dept. Genetica e Biol. Molecolare, Università La Sapienza, P.le A.Moro 5, 00185 Roma-Italy

  • 14-3
    •   The protein kinase Hsl1 binds to the yeast mitotic septins.
    Björn Gullbrand (1), Matthias Versele (1), Victor Cid (2), Jeremy Thorner (1)
    (1) Molecular and Cell Biology, UC Berkeley, 401 Barker Hall, Berkeley, CA 94720 3202, USA (bjorng@uclink.berkeley.edu); (2) Departamento de Microbiologia II, Universidad Complutense de Madrid, 28040 Madrid, Spain

  • 14-4
    •   Using yeast as a model to demonstrate a new mechanism of sulfa drug action.
    Onisha Patel (1), Ross Fernley (1), Ian Macreadie (1), Peter Coloe (2), Jacquie Satchell (3), Johnathan Baell (3)
    (1) Structural biology, CSIRO Health Sciences & Nutrition, 343 Royal Parade, Melbourne, 3052, Australia (Onisha.Patel@csiro.au); (2) RMIT University, Melbourne, Australia; (3) Walter and Eliza Hall Institute, Melbourne, Australia

  • 14-5
    •   The protein kinase Ime2 and the regulation of the anaphase-promoting complex during meiosis.
    Melanie Bolte, Patrick Dieckhoff, Gerhard H. Braus, Stefan Irniger
    Moleculare Microbiology, Inst of Microbiology Genetics, Grisebachstr. 8, Goettingen, D-37077, Germany (mbolte@gwdg.de)

  • 14-6
    •   The effect of new methanesulfonylhydrazones on yeast Saccharomyces cerevisiae cultures.
    Ekaterina Peicheva, Nicolay Dodoff, George Miloshev
    Department Molecular Genetics, Institute of Molecular Biology, Acad. G. Bonchev 21, Sofia, 1113, Bulgaria (ekpeicheva@yahoo.com)

  • 14-7
    •   Orchestrating Chromosome Segregation During Mitosis.
    Toru Higuchi, Nadine Hornig, Matt Sullivan, Frank Uhlmann
    Lincoln's Inn Fields Laborator, Cancer Research UK, Lincoln's Inn Fields, London, WC2A 3PX, UK (frank.uhlmann@cancer.org.uk)

  • 14-8
    •   Gpr1-Gpa2 signaling pathway regulates glucose dependent cell size.
    Hisanori Tamaki, Cheol-Won Yun, Makiko Shinozaki, Hidehiko Kumagai
    Grad. Sch. of Biostudies, Kyoto University, Kitashirakawa, Kyoto, 606-8502, Japan (noritama@kais.kyoto-u.ac.jp)

  • 14-9
    •   Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast.
    Satoshi Yoshida, Ryuji Ichihashi, Akio Toh-e
    Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Tokyo, 113-0033, Japan (toh-e@biol.s.u-tokyo.ac.jp)

  • 14-10
    •   Fission yeast PI/PC transfer protein, Spo20, is required for integrity of meiotic spindle pole bodies.
    Yukiko Nakase (1), Taro Nakamura (1), Koei Okazaki (2), Chikashi Shimoda (1)
    (1) Dept. Biol., Grad. Sch. Sci., Osaka City University, Sumiyoshi-ku, Osaka, 558-8585, Japan (shimoda@sci.osaka-cu.ac.jp); (2) Kisarazu, Chiba, Japan

  • 14-11
    •   Overproduction of the Schizosaccharomyces pombe Rpg1 protein in Saccharomyces cerevisiae causes cell cycle delay.
    Ivana Janatova, Zdenek Koubek, Katerina Malinska, Radka Rakova, Jiri Hasek
    Lab. of Cell Reproduction, Institute of Microbiology, Videnska 1083, Prague 4, Cz-14220, Czech Republic (janatova@biomed.cas.cz)

  • 14-12
    •   Analysis of B-type cyclin Cdc13-binding protein Nap1 in fission yeast.
    Maribel Grande, Sandra López-Avilés, Oriol Bachs, Rosa Aligue
    Cell Biology, University of Barcelona, C/ Casanova 143, Barcelona, 08036, Sapin (migrande@medicina.ub.es)

  • 14-13
    •   Mechanism of antagonistic growth regulation by the high-osmolarity glycerol pathway and Ca 2+-signaling pathways in budding yeast.
    Atsunori Shitamukai, Toshinaga Yamaguchi, Dai Hirata, Tokichi Miyakawa
    Molecular Biotechnology, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, 739-8530, Japan (ashita@hiroshima-u.ac.jp)

  • 14-14
    •   The Saccharomyces cerevisiae Sda1p is required for the entry into a new cell cycle and is localized in the nucleololus.
    Francesca Saracino, Sarah Gobbi, Diego Muzzini, Maria Luisa Agostoni Carbone
    Dipartimento di Genetica, Università di Milano, via Celoria 26, Milano, 20133, Italy (saracino@mailserver.unimi.it)

  • 14-15
    •   Functional analysis of piD261/Bud32, a yeast atypical protein kinase conserved during evolution.
    Raffaele Lopreiato, Geppo Sartori, Sonia Facchin, Lorenzo A. Pinna, Giovanna Carignani
    Dept. of Biological Chemistry, University of Padova, via G. colombo 3, Padova, 35121, Italy (lopreiat@mail.bio.unipd.it)

  • 14-16
    •   ROT1, a gene functionally linked to the PKC pathway, is required for proper mitotic exit.
    Ethel Queralt, M. Angeles Juanes, M. Carmen Bañó, J. Carlos Igual
    Dept. Bioquimica i Biol. Mol., Universitat de València, Dr. Moliner, 50, Valencia, 46100, Spain (equeralt@uv.es)

  • 14-17
    •   Using Yeast to Dissect Sulfa Drug Resistance Mechanisms.
    Peter Iliades (1), Steven Meshnick (2), Ian Macreadie (1)
    (1) Health Sciences and Nutrition, CSIRO, 343 Royal Parade, Melbourne, 3052, Australia (peter.iliades@csiro.au); (2) University of North Carolina, Chapel Hill, North Carolina, USA

  • 14-18
    •   The 14-3-3 proteins Rad24 and Rad25 negatively regulate Byr2 by affecting its localization in Schizosaccharomyces pombe.
    Yasuyo Kobayashi, Fumiyo Ozoe, Makoto Kawamukai
    Life and Environmental Science, Shimane University, 1060, Matsue, 690-8504, Japan (kawamuka@life.shimane-u.ac.jp)

  • 14-19
    •   A novel function of Saccharomyces cerevisiae PKC1 in the regulation of polarized bud growth.
    Masaki Mizunuma, Dai Hirata, Tokichi Miyakawa
    Molecular Biotechnology, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima, 739-8530, Japan (mmizu@hiroshima-u.ac.jp)

  • 14-20
    •   Role and regulation of the Mad3 spindle assembly checkpoint protein in budding yeast.
    Giulia Rancati, Giovanna Lucchini, Simonetta Piatti
    Biotecnologie e Bioscienze, Università di Milano Bicocca, Piazza della Scienza, Milano, 20100, Italy (giulia.rancati@unimib.it)

  • 14-21
    •   Budding yeast PAK kinases regulate mitotic exit by two different mechanisms.
    Elena Chiroli, Roberta Fraschini, Giovanna Lucchini, Simonetta Piatti
    Biotecnologie e Bioscienze, Università di Milano Bicocca, piazza della Scienza, Milano, 20100, Italy (elena.chiroli@unimib.it)

  • 14-22
    •   Regulatory mechanisms of mitotic checkpoint kinases.
    Eric P. Holinger, Mark Winey
    MCDBiology, University of Colorado-Boulder, CB347, Boulder, CO 80309, USA (holinger@colorado.edu)

  • 14-23
    •   Far1 is a component of the cell sizer mechanism involved in the G1/S transition in vegetatively growing S. cerevisiae cells.
    Marco Vanoni, Lorenzo Querin, Riccardo L Rossi, Valeria Wanke, Lilia Alberghina
    Dip.Biotecnologie e Bioscienze, Univ. di Milano-Bicocca, P. della Scienza 2, Milano, 20126, Italy (marco.vanoni@unimib.it)

  • 14-24
    •   Adaptive evolution of Saccharomyces cerevisiae under nitrogen-limited conditions.
    Renata Usaite, Birgitte Regenberg, Mats Äkesson, Jens Nielsen
    CPB, BioCentrum-DTU, Technical Univ of Denmark, Building 223, Kgs. Lyngby, DK-2800, Denmark (ru@biocentrum.dtu.dk)

  • 14-25
    •   Asynchronous mitoses in a multinucleated cell.
    Amy Gladfelter, Hans Peter Helfer, Katrin Hungerbuehler, Peter Philippsen
    Molecular Microbiology, University of Basel Biozentrum, Klingelberstrasse 50, Basel, 4056, Switzerland (amy.gladfelter@unibas.ch)

  • 14-26
    •   The S. cerevisiae 14-3-3 homolog Bmh1 has multiple roles in DNA metabolism and DNA damage checkpoint response.
    Francisca Lottersberger, Fabio Rubert, Giovanna Lucchini, Maria Pia Longhese
    Biotecnologie e Bioscienze, Università di Milano-Bicocca, P.za della Scienza 2, Milano, 20126, Italy (franciscalottersberger@yahoo.com)

  • 14-27
    •   Interaction of TOR signaling and histone acetylase complexes at ribosomal protein promoters.
    Maria Cardenas, John Rohde
    Mol. Genetics & Microbiology, Duke University, Research Dr, Bx 3546, Durham, NC 27710, USA (carde004@mc.duke.edu)

  • 14-28
    •   Characterization of the Rapamycin-sensitive TOR complex 1 in S. cerevisiae.
    Anja Lorberg, Robbie Loewith, José-Luis Crespo, Wolfgang Oppliger, Thierry Mini, Paul Jenö, Michael N. Hall
    Division of Biochemistry, Biozentrum, Klingelbergstr.70, Basel, 4056, Switzerland (anja.lorberg@unibas.ch)

  • 14-29
    •   Swm1p and Mnd2p are new components of the budding yeast anaphase-promoting complex.
    Andrew M. Page (1), Vicky Aneliunas, Ben Montpetit, Karen Yuen (1), Christopher Carroll (2), Brian Snydsman (3), Eric G. Muller, Trisha N. Davis (3), John R. Lamb (4), David O. Morgan (2), Hieter Philip (1)
    (1) CMMT, University of British Columbia, 980 W. 28th Ave., Vancouver, BC V5Z4H4, Canada (apage@cmmt.ubc.ca); (2) Department of Physiology, University of California, San Francisco, CA, USA; (3) Department of Biochemistry, University of Washington, Seattle, WA; (4) Fred Hutchinson Cancer Institute, Seattle, WA, USA

  • 14-30
    •   Functional analysis of ccp1 +, a novel spindle pole body component in fission yeast Schizosaccharomyces pombe.
    Kayoko Tanaka, Nobuhiro Nonaka, Masayuki Yamamoto
    Graduate school of Science, University of Tokyo, 7-3-1 Hongo, Tokyo, 113, Japan (ktanaka@ims.u-tokyo.ac.jp)

  • 14-31
    •   Pho85 kinase is involved in the diauxic shift of the yeast Saccharomyces cerevisiae.
    Masafumi Nishizawa (1), Akio Toh-e (2)
    (1) Dept. Microbiology, Keio University Sch Med, Shinjuku, Tokyo, 160-8582, Japan (mas@sc.itc.keio.ac.jp); (2) Dept. Biological Sciences, The University of Tokyo Graduate School of Science, Bunkyo, Tokyo, Japan

  • 14-32
    •   Fission yeast meiotic regulator Mei2p forms a dot structure in association with the sme2 locus on chromosome II during meiotic prophase.
    Tadayuki Shimada (1), Akira Yamashita (2), Masayuki Yamamoto (1)
    (1) Dept Biophysics & Biochemistry, Univ of Tokyo Schl of Science, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan (myamamot@ims.u-tokyo.ac.jp); (2) Molecular Genetics Research Lab, Univ of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

  • 14-33
    •   Mes1p is a meiotic regulator that interacts with and inhibits Slp1p in fission yeast.
    Daisuke Izawa (1), Masuo Goto (1), Akira Yamashita (2), Masayuki Yamamoto (1)
    (1) Dept Biophysics & Biochemistry, Univ of Tokyo Schl of Science, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan (d-180sx@ims.u-tokyo.ac.jp); (2) Molecular Genetics Research Lab, Univ of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

  • 14-34
    •   Meiotic differentiation in yeast colonies: a model for boundary formation?
    Kedar Purnapatre, Sarah Piccirillo, Misa Gray, Jessica Kueker, Douglas Law, Saul Honigberg
    Div. Cell Biology & Biophysics, Univ. of Missouri-Kansas City, 5007 Rockhill Rd, Kansas City, MO 64110-2499, USA (honigbergs@umkc.edu)

  • 14-35
    •   mRNA stability and translational control: two new ways of keeping the Cdk inhibitor Rum1 under control.
    Rafael Daga, Pilar Bolanos, Sergio Moreno
    Cancer Research Institute CSIC, Universidad de Salamanca, Campus Miguel de Una, Salamanca, 37007, Spain (smo@usal.es)

  • 14-36
    •   Screening for upstream components of the TOR pathway using pathway-activating TAP42 mutants.
    Tatsuya Maeda, Tatsuya Umeda, Miyako Yoneyama, Masao Kamiya, Michio Hayashi
    Inst. Mol. Cell. Biosci., The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan (maeda@ims.u-tokyo.ac.jp)

  • 14-37
    •   The role of phosphorylation in glucose-induced inactivation in Saccharomyces Maltose Permease.
    Nidhi Gadura (1), Corinne A. Michels (1), Lucy C. Robinson (2)
    (1) Graduate School of CUNY, Queens College, Biology, 65-30 Kissena Blvd., Flushing, NY 11367, USA (nidhigadura@hotmail.com); (2) 1501 Kings Highway, Shreveport, LA 71130, USA

  • 14-38
    •   Ipl1p protein kinase and type 1 protein phosphatase function antagonistically in the bipolar attachment of chromosomes to the mitotic spindle.
    Najma Rachidi, Amanda J. Gardiner, Michael J. R. Stark
    School of Life Sciences, University of Dundee, MSI/WTB Complex, Dundee, DD1 5EH, United Kingdom (m.j.r.stark@dundee.ac.uk)

  • 14-39
    •   Mum2 is required for normal meiotic progression and interacts with Ime4 and Kar4, proteins with similarity to RNA methyltransferases.
    Stewart Morgan, JoAnne Engebrecht
    Pharmacology, Suny @ Stony Brook, Stony Brook Univ., Stony Brook, NY 11794-8651, USA (morgan@pharm.sunysb.edu)

    Poster Session 15 - Systematic assessment of function

  • 15-1
    •   Assessment of uncharacterised genes of Saccharomyces cerevisiae implicated in meiotic DNA processing pathways by integration of high-throughput genetic data.
    Philip Jordan
    Institute of Cellular and Molecular Biology, University of Edinburgh, Mayfield Road, EH9 3JR, Edinburgh, Scotland, (p.jordan@ed.ac.uk)

  • 15-2
    •   Genome-wide gene deletion screen for components involved in mitochondrial biogenesis.
    Benedikt Westermann (1), Kai Stefan Dimmer (1), Marlies Messerschmitt (1), Stefan Fritz (1), Nadja Weinbach (1), Florian Fuchs (1), Stefan Jakobs (2), Frank Vogel (3)
    (1) Physiologische Chemie, Universitaet Muenchen, Butenandtstr. 5, Muenchen, 81377, Germany (benedikt.westermann@bio.med.uni-muenchen.de); (2) Max-Planck-Institut fuer Biophysikalische Chemie, Goettingen, Germany; (3) Max-Delbrueck-Centrum fuer Molekulare Medizin, Berlin, Germany

  • 15-3
    •   Identification of phosphoglycerate dehydrogenases in Saccharomyces cerevisiae.
    Eva Albers (1), Vincent Laizé (2), Anders Blomberg (3), Stefan Hohmann (3), Lena Gustafsson (1)
    (1) Chemistry and Bioscience, Chalmers Univ. of Technology, Box 462, Göteborg, SE-405 30, Sweden (Eva.Albers@molbiotech.chalmers.se); (2) Universidade do Algarve, Centro de Ciências do Mar, Campus de Gambelas, P-8000-117 Faro, Portugal; (3) Department of Cell and Molecular Biology, Göteborg University, Box 462, SE-405 30 Göteborg, Sweden

  • 15-4
    •   Genomic Screen for new vacuole protein sorting mutants in Saccharomyces cerevisiae.
    Cecilia Bonangelino, Edna Chavez, Juan Bonifacino
    CBMB, NICHD, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20982, USA (cbonange@helix.nih.gov)

  • 15-5
    •   Design methods for targeted integration cassettes and autonomous plasmids in Schizosaccharomyces pombe.
    Lili Wang, Richard Kao, F. Douglas Ivey, David A. Kelly, Charles S. Hoffman
    Biology Department, Boston College, 140 Commonwealth Ave, Chestnut Hill, MA 02467, USA (hoffmacs@bc.edu)

  • 15-6
    •   Phenotype MicroArray analysis of S. cerevisiae strains with knockouts of human disease gene homologs.
    Barry Bochner
    R&D, Biolog, Inc., 3938 Trust Way, Hayward, CA 94545, USA (bbochner@biolog.com)

  • 15-7
    •   Systematic analysis of protein domain interactions in Saccharomyces cerevisiae.
    Peter Uetz, Carolina S. Vollert, Claudia Dambmann, Sebastian Heucke, Seesandra V. Rajagopala, Tanja Schüler
    Institute of Genetics, Forschungszentrum Karlsruhe, Box 3640, Karlsruhe, 76021, Germany (peter@uetz.de)

  • 15-8
    •   Using yeast to place foreign genes into different functional categories.
    Nianshu Zhang (1), Mike Osborn (2), Paul Grisham (1), Jill Wishart (1), Kuangyu Yen (1), Zorana Vujovic (1), Claire-Louise Palmer (3), Andrew M. Bailey (3), Ross Miller (2), Stephen G. Oliver (1)
    (1) School of Biological Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK (nzhang@man.ac.uk); (2) Molecular Immunology Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, UK; (3) School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK

  • 15-9
    •   Transcript profiling of peroxisome-associated genes in anaerobically grown Saccharomyces cerevisiae cells.
    Marek Skoneczny (1), Anna Chelstowska (1), Ronald A. Butow (2)
    (1) Department of Genetics, Inst of Biochem & Biophysics, Pawinskiego 5A, Warszawa, 02-106, Poland (kicia@ibb.waw.pl); (2) Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9148, USA

  • 15-10
    •   Isolation of multiprotein complex(es) made by Prs proteins of Saccharomyces cerevisiae.
    Stefano Vavassori, Lilian M. Schweizer, Michael Schweizer
    Heriot-Watt University, School of Life Sciences, Riccarton, Edinburgh, EH14 4AS, UK (S.L.Vavassori@hw.ac.uk)

  • 15-11
    •   A Bayesian networks approach to predict protein complexes from genomic data.
    Ronald Jansen (1), Haiyuan Yu (2), Dov Greenbaum (2), Yuval Kluger (2), Nevan Krogan (3), Sambath Chung (2), Michael Snyder (2), Andrew Emili (3), Jack Greenblatt (3), Mark Gerstein (2)
    (1) Computational Biology Center, Sloan-Kettering Institute, 307 East 63rd Street, New York, NY 10021, USA (jansenr@mskcc.org); (2) Department of Molecular Biophysics & Biochemistry, 266 Whitney Avenue, Yale University, PO Box 208114, New Haven, CT 06520, USA; (3) Banting and Best Department of Medical Research, Department of Molecular and Medical Research, University of Toronto, Toronto, M5G 1L6, Ontario, Canada

    Poster Session 16 - Comparing genomes

  • 16-1
    •   Taxonomic investigation of Saccharomyces boulardii.
    Laura C Edwards, Lubomira Stateva
    Biomolecular Sciences, UMIST, PO Box 88, Manchester, M60 1QD, UK (L.Edwards@postgrad.umist.ac.uk)

  • 16-2
    •   Influence of tandemly repeated sequences on genome dynamics: study of the molecular evolution of the Saccharomyces cerevisiae DUP240 family.
    Bénédicte Wirth, Laurence Despons, Véronique Leh-Louis, Serge Potier, Jean-Luc Souciet
    FRE 2326 ULP/CNRS, Institut de Botanique, 28, rue Goethe, Strasbourg, 67083, France (wirth@gem.u-strasbg.fr)

  • 16-3
    •   Characterization of the retrotransposon Ylt1 of the yeast Yarrowia lipolytica.
    Andriy Kovalchuk, Senam Senam, Gerold Barth
    Institut für Mikrobiologie, Technische Universität Dresden, Mommsenstr. 13, Dresden, D-01062, Germany (andriy.kovalchuk@mailbox.tu-dresden.de)

  • 16-4
    •   Diversity of the chromosomal structure in lager brewing yeasts.
    Yukiko Kodama (1), Yoshihiro Nakao (1), Norihisa Nakamura (1), Tomoko Fujimura (1), Katsuhiko Shirahige (2), Toshihiko Ashikari (1)
    (1) Inst. for Advanced Technology, Suntory Research Center, 1-1-1, Wakayamadai, Mishima, Osaka, 618-8503, Japan (Yukiko_Kodama@suntory.co.jp); (2) Riken Genomic Science Center,1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

  • 16-5
    •   How to use genome-wide expression data to learn from yeast about gene regulation in higher eukaryotes.
    Sven Bergmann, Jan Ihmels, Naama Barkai
    Molecular Genetics, Weizmann Institute of Science, Mayer 404, Rehovot, 76100, Israel (Sven.Bergmann@weizmann.ac.il)

  • 16-6
    •   Transfer of proteomic knowledge from Saccharomyces cerevisiae to Debaryomyces hansenii.
    Klaus Gori, Henrik Dam Mortensen, Nils Arneborg, Lene Jespersen
    Dpt. of Dairy and Food Science, Royal Vet. & Agric. University, Rolighedsvej 30, Frederiksberg C, DK-1958, Denmark (klg@kvl.dk)

  • 16-7
    •   Origins of the yeast genome.
    Jure Piskur
    BioCentrum-DTU, Danmarks Tekniske Universitet, Building 301, Lyngby, DK-2800, Denmark (jp@biocentrum.dtu.dk)

  • 16-8
    •   The dimorphic yeast Yarrowia lypolytica genome sequencing project.
    Serge Casaregola (1), Cecile Neuveglise (1), Patrick Wincker (2), Veronique Anthouard (2), Jean Weissenbach (2), Claude Gaillardin (1), The Yarrowia lipolytica genome sequencing consortium (3), The Genolevures II consortium (3)
    (1) Microbiologie Moleculaire et Cellulaire, UMR 1238 INRA, CNRS, INA-PG, 78850 Thiverval-Grignon, France (serge@grignon.inra.fr); (2) Genoscope-Centre National de Sequençage, 2 rue Gaston Crémieux, 91057 Evry, France; (3) various laboratories

  • 16-9
    •   Accelerated evolution of some gene copies after genome duplication in Saccharomyces.
    Mario A. Fares, Kenneth H. Wolfe
    Department of Genetics, Trinity College, Dublin, 2, Ireland (faresm@tcd.ie)

  • 16-10
    •   The DAL gene cluster - gathered or scattered?
    Simon Wong, Kenneth H. Wolfe
    Department of Genetics, Trinity College, Dublin, 2, Ireland (swong@tcd.ie)

  • 16-11
    •   Differential evolution of the Saccharomyces cerevisae DUP240 paralogs, implication of recombination in phylogeny reconstructions.
    Laurence Despons (1), Bénédicte Wirth (1), Véronique Leh-Louis (1), Simon Wain-Hobson (2), Serge Potier (1), Jean-Luc Souciet (1)
    (1) FRE 2326 ULP/CNRS, Institut de Botanique, 28 rue Goethe, Strasbourg, 67083, France (despons@gem.u-strasbg.fr); (2) Unité de Rétrovirologie Moléculaire, Institut Pasteur, 28 rue du Dr Roux, F-75724 Paris Cedex 15, France

  • 16-12
    •   Classification of yeasts using array-based comparative genomic hybridization.
    Hiroyuki Yoshimoto (1), Rie Ohuchi (1), Tatsuji Ishiguro (1), Keiko Tanaka (1), Satoru Mizutani (1), Kosuke Tashiro (2), Satoru Kuhara (2), Osamu Kobayashi (1)
    (1) Central Labs. for Key Tech., Kirin Brewery Co., Ltd., Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan (hyoshimoto@kirin.co.jp); (2) Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan

  • 16-13
    •   Sequencing and Analysis of the Hansenula polymorpha Genome.
    Massoud Ramezani-Rad (1), Cornelis P. Hollenberg (1), Juergen Lauber, Holger Wedler (2), Eike Griess (2), Christian Wagner, Kaj Albermann (3), Jean Hani (3), Michael Piontek (4), UlrikeDahlems, Gerd Gellissen (5)
    (1) Institute for Microbiology, Heinrich-Heine University, Universitätsstr. 1, Düsseldorf, 40225, Germany (ramezani@uni-duesseldorf.de); (2) QIAGEN Genomic Services, QIAGEN GmbH, Max-Volmer Str. 4, D-40724 Hilden, Germany; (3) Biomax Informatics AG, Lochhamer Str. 11, D-82152 Martinsried, Germany; (4) ARTES Biotechnology GmbH, Agnesstr. 8, D-45136 Essen, Germany; (5) Rhein Biotech GmbH, Eichsfelder Strasse 11, D-40595 Düsseldorf, Germany

  • 16-14
    •   Comparison of three Saccharomyces mitochondrial DNA sequences.
    Rikke B. Langkjær (1), Serge Casaregola (2), David W. Ussery (1), Claude Gaillardin (2), Jure Piskur (1)
    (1) BioCentrum-DTU, Building 301, DK-2800 Kgl. Lyngby, Denmark; (2) Microbiologie et Genetique Moleculaire, INRA CNRS INA-PG, 78850 Thiverval-Grignon, France (serge@grignon.inra.fr)

  • 16-15
    •   Analysis of the MAL and the SUC loci repeated in a bottom fermenting yeast genome.
    Osamu Kobayashi (1), Shoko Nakagawa (1), Keiko Tanaka (1), Tatsuji Ishiguro (1), Emiko Shimada (1), Satoru Mizutani (1), Naoyuki Umemoto (2)
    (1) Central Labs. for Key Tech., Kirin Brewery Co., Ltd, Fukuura, Kanazawa-ku, Yokohama-shi, 236-0004, Japan (osamuk@kirin.co.jp); (2) Plant Lab., Kirin Brewery Co., Ltd, Kitsuregawa-machi, Shioya-gun, Tchigi-ken, 329-1491 Japan

  • 16-16
    •   Defining Saccharomyces Genes.
    J. Michael Cherry, Chandra Theesfeld, Anand Sethuraman, Dianna G. Fisk, Kara Dolinski, Rama Balakrishnan, Gail Binkley, Karen R. Christie, Maria Costanzo, Stan Dong, Selina S. Dwight, Stacia Engel, Jodi Hirschman, Eurie L. Hong, Laurie Issel-Tarver, Shuai Weng, David Botstein
    Department of Genetics, Stanford University, Medical Center, Stanford, CA 94305-5120, USA (cherry@stanford.edu)

  • 16-17
    •   Inferring gene function from genome comparison.
    Jacques van Helden
    SCMBB CP263, Universite Libre de Bruxelles, bould du Triomphe, Bruxelles, B-1050, Belgium (jacques.van.helden@ulb.ac.be)

  • 16-18
    •   Evolution of superfamily of alpha-galactosidase MEL genes in the Saccharomyces sensu stricto complex.
    Elena S. Naumova, Gennadi I. Naumov
    Lab. of Yeast Genetics, GosNIIgenetika, I-Dorozhnyi 1, Moscow, 117545, Russia (gnaumov@yahoo.com)

  • 16-19
    •   Comparative evolutionary genomics of CUG reassignment in Candida species.
    Manuel Santos (1), Pedro Beltrão (2), Ricardo Almeida (2), Steven Massey (3), James Garey (2), Gabriela Moura (1)
    (1) Centre for Cell Biology, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal (msantos@bio.ua.pt); (2) Biology, University of Aveiro, Santiago Uni. Campus, Aveiro, 3810-193, Portugal; (3) Department of Biology, University of South Florida, Tampa, Florida 33620, USA

  • 16-20
    •   Comparative and evolutionary mitochondrial genomics of fission yeasts.
    Jessica Leigh, Charles Bullerwell, B. Franz Lang
    Biochemistry, Universite de Montreal, 2900, Ed. Montpetit, Montreal, H3C 3J7, Canada (Franz.Lang@Umontreal.ca)

  • 16-21
    •   Saccharomyces comparative genomics: Genes, regulatory motifs, and genome evolution.
    Manolis Kamvysselis, Nick Patterson, Bruce Birren, Eric Lander
    Center for Genome Research, MIT / Whitehead Institute, 320 Charles St, Cambridge, MA 02139, USA (manoli@mit.edu)

    Poster Session 17 - Beyond functional analysis: systems biology

  • 17-1
    •   A deterministic molecular model of the fission yeast cell cycle.
    Akos Sveiczer (1), Attila Csikasz-Nagy (1), John J. Tyson (2), Bela Novak (1)
    (1) Dept Agricult Chem Technol, Budapest University Techn Econ, Szt. Gellert ter 4, Budapest, H-1111, Hungary (asveiczer@mail.bme.hu); (2) Dept Biology, Virginia Tech, Blacksburg, VA 24061, USA

  • 17-2
    •   Metabolic engineering in Saccharomyces cerevisiae through the use of a reconstructed genome-scale metabolic network leads to improved ethanol production.
    Christoffer Bro (1), Birgitte Regenberg (1), Jochen Förster (2), Jens Nielsen (1)
    (1) Center for Process Biotechnology, BioCentrum-DTU, Building 223, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; (2) Center for Process Biotechnology, BioCentrum-DTU, Building 223, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark, Fluxome Sciences A/S, Building 223, Søltofts Plads, DK-2800 Kgs. Lyngby, Denmark. (jochen.forster@fluxome.com)

  • 17-3
    •   The weak organic acid stress response in yeast: Pdr12p and War1p are necessary and suffcient for stress adaptation.
    Karl Kuchler
    Medical Biochemistry, Campus Vienna Biocenter, Dr. Bohr-Gasse 9/2, Vienna, A-1030, Austria (kaku@mol.univie.ac.at)

  • 17-4
    •   Elucidation of the interaction network between yeast DNA processing proteins using endogenously tagged ORFs and the LiquiChip technology.
    Mike Fetchko, Ingrid Studer, Jacqueline Hort, Igor Stagljar
    I. of V. Bioch. & Mol. Biol., University of Zurich, Winterhrurerstr. 190, Zürich, 8057, Switzerland (stagljar@vetbio.unizh.ch)

  • 17-5
    •   Yeast expression at your fingertips.
    Jan Ihmels, Sven Bergmann, Naama Barkai
    Molecular Genetics, Weizmann Institute of Science, Meyer Bldg, Rehovot, 76100, Israel (jan@weizmann.ac.il)

  • 17-6
    •   Metabolic characterization of regulatory knockout mutants of Saccharomyces cerevisiae.
    Lars M. Blank, Uwe Sauer
    Institute of Biotechnology, ETH Zürich, Hönggerberg, Zürich, 8093, Switzerland (blank@biotech.biol.ethz.ch)

  • 17-7
    •   Molecular and computational analysis of a cell sizer network controlling entrance into S phase in budding yeast.
    Riccardo L. Rossi (1), Lorenzo Querin (1), Simone Paleari (2), Antonio Giorgilli (2), Marco Vanoni (1), Lilia Alberghina (1)
    (1) Dip.Biotecnologie e Bioscienze, Univ. di Milano-Bicocca, P. della Scienza 2, Milano, 20126, Italy (riccardo.rossi@unimib.it); (2) Dip. Matematica e Applicazioni, Univ. di Milano-Bicocca, P. dell'Ateneo Nuovo 1, 20126 Milano, Italy

  • 17-8
    •   A model identification algorithm for cell signalling pathways.
    Peter Gennemark (1), Bodil Nordlander (2), Per Sunnerhagen (2), Stefan Hohmann (2), Dag Wedelin (1)
    (1) Computing Science, Chalmers Univ. of Tech., Eklandagatan 86, Göteborg, SE-412 96, Sweden (peterg@cs.chalmers.se); (2) Cell and Molecular Biology, Göteborg University, Box 462, SE-405 30 Göteborg, Sweden.

  • 17-9
    •   Mathematical modeling of signal transduction in yeast.
    Edda Klipp
    Dept. Vertebrate Genomics, Max-Planck Institute MolGen, Ihnestr. 73, Berlin, 14195, Germany (klipp@molgen.mpg.de)

  • 17-10
    •   The regulatory network of glucose repression in Saccharomyces cerevisiae.
    Steen Lund Westergaard (1), Christoffer Bro (1), Steen Knudsen (2), Lisbeth Olsson (1), Jens Nielsen (1)
    (1) Process Biotechnology, Tech. University of Denmark, Soeltofts Plads 223, Kgs Lyngby, DK-2800, Denmark (slw@biocentrum.dtu.dk); (2) Center for Biological Sequence Analysis, Techn. University of Denmark, Kemitorvet 208, DK-2800 Kgs Lyngby, Denmark

  • 17-11
    •   Integrated analysis of glycolytic rate control - a systems biology approach.
    Mikael Johansson (1), Milena Anguelova (2), Bernt Wennberg (2), Carl Johan Franzén (1)
    (1) Chemical Engineering and Environmental Science, Chalmers University of Technology, SE-412 96 Göteborg, Sweden (franzen@cre.chalmers.se); (2) Mathematics, Chalmers University of Technology, SE-412 96 Göteborg, Sweden

  • 17-12
    •   Systems biology approaches in budding yeast.
    Lilia Alberghina
    Biotechnology and Biosciences, Univ. Milano-Bicocca, Pzza della Scienza 2, Milano, 20126, Italy (lilia.alberghina@unimib.it)

  • 17-13
    •   System-oriented modeling of Saccharomyces cerevisiae : coupling cell cycle progression and energy metabolism.
    Dirk Mueller, Luciano Aguilera-Vázquez, Ester Guerrero-Martín, Matthias Reuss
    Inst. of Biochem. Engineering, University of Stuttgart, Allmandring 31, Stuttgart, D-70569, Germany (Mueller@ibvt.uni-stuttgart.de)

  • 17-14
    •   Expanding the role of Reciprocal Hemizygosity Scanning (RHS).
    Jed Dean (1), John McCusker (2), Ron Davis (1), Lars Steinmetz (1)
    (1) Biochemistry, Stanford Medical Center, Beckman B400, Stanford, CA 94305-5307, USA (ejdean@stanford.edu); (2) Department of Microbiology, 3020, Duke University Medical Center, Durham, NC 27710, USA

  • 17-15
    •   Network motifs: simple building blocks of transcription networks.
    Uri Alon
    Molecular Cell Biology, Weizmann Institute of Science, Herzel 1, Rehovot, 76100, Israel (urialon@weizmann.ac.il)

    Poster Session 18 - Yeast apoptosis and ageing

  • 18-1
    •   Yeast cell - a system to study the action of a neurotoxic phospholipase A 2.
    Uros Petrovic, Igor Krizaj, Franc Gubensek
    Biochemistry and Mol. Biology, Jozef Stefan Institute, Jamova 39, Ljubljana, SI-1000, Slovenia (uros.petrovic@ijs.si)

  • 18-2
    •   Two-level structure of the [PSI+] prion particles.
    Dmitry S. Kryndushkin, Ilya M. Alexandrov, Vitaly V. Kushnirov, Michael D. Ter-Avanesyan
    Molecular Genetics Laboratory, Cardiology Research Center, 3rd Cherepkovskaya, Moscow, 121552, Russia (dkrynd@cardio.ru)

  • 18-3
    •   Prion forming abilities of interspecies recombinant Sup35p.
    Julia V. Sopova, Sergey P. Zadorsky, Sergey G. Inge-Vechtomov
    Dept. of Genetics and Breeding, St-Petersburg State University, Universitetskaya nab, St-Petersburg, 199034, Russia (sopova@hotmail.com)

  • 18-4
    •   Novel antisuppressor determinant [ ASP + ] of the yeast Saccharomyces cerevisiae.
    Veniamin A. Startsev, Julia V. Sopova, Sergey P. Zadorsky, Sergey G. Inge-Vechtomov
    Dep. of Genetics and Breeding, St-Petersburg State University, Universitetskaja nab, St-Petersburg, 199034, Russia (startsev@pochtamt.ru)

  • 18-5
    •   The use of centromeric gene library for identification of genes responsible for the [ISP +] prion-like determinant maintenance.
    Anna Aksenova, Kirill Volkov, Anton Svitin, Ludmila Mironova
    Department of Genetics, St. Petersburg University, University emb. 7/9, Saint-Petersburg, 199034, Russia (anton@btc.bio.pu.ru)

  • 18-6
    •   Identification of heterologous multicopy suppressors of the yeast msb3msb4 double mutation.
    Jean-Marc Delroisse, Martine Breban, Arsène Burny, Daniel Portetelle, Micheline Vandenbol
    Unité de Microbiologie, FUSAGx, Av. Maréchal Juin, 6, Gembloux, B-5000, Belgium (delroisse.jm@fsagx.ac.be)

  • 18-7
    •   Genetic interactions between SGS1 and SRS2, encoding DNA helicases required for maintenance of genomic stability in yeast.
    Hocine W. Mankouri (1), Alan Morgan (2), Ian D. Hickson (1)
    (1) Institute of Mol. Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 8NW, England (h.mankouri@cancer.org.uk); (2) Department of Physiology, PO Box 147, Crown Street, University of Liverpool, Liverpool, L69 3BX, England

  • 18-8
    •   Ure2, a prion precursor with homology to glutathione S-transferase, protects Saccharomyces cerevisiae cells from heavy metal ion and oxidant toxicity.
    Rajendra Rai, Jennifer J. Tate, Terrance G. Cooper
    Molecular Sciences, University of Tennessee, 858 Madison Ave., Memphis, TN 38163, USA (jtate@utmem.edu)

  • 18-9
    •   The test-system for phenotypic detection of PrP aggregation in yeast.
    Alexey Galkin (1), Alexandr Rubel (1), Viktoria Navolotskaya (1), Anton Markov (1), Yury Chernoff (2), Sergey Inge-Vechtomov (1), Alexandr Smirnov (1)
    (1) Dept. of Genetics and Breeding, St.-Petersburg University, Universitetskaya nab, Saint-Petersburg, 199034, Russia (apgalkin@hotbox.ru); (2) School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, M/C 0363, 315 Ferst Drive, Atlanta, GA 30332-0363, USA

  • 18-10
    •   Induction of the yeast Saccharomyces cerevisiae prion [ PSI + ] by chimeric protein Sup35N-Ade2.
    Andrey S. Borchsenius (1), Stanislav V. Zharkevich (1), Natalia A. Ryabinkova (1), Vladimir V. Alenin (1), Kyastas Sasnauskas (2), Gary P. Newnam (3), Yury O. Chernoff (3), Sergey G. Inge-Vechtomov (1)
    (1) Dept. of Genetics and Breeding, St.Petersburg State University, Universitetskaya 7/9, Saint-Petersburg, 199034, Russian Federarion (aborchsenius@yahoo.com); (2) Institute of Biotechnology, Vilnius, LT-2028, Lithuania; (3) School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Dr., Atlanta GA, 30332, USA.

  • 18-11
    •   Regulation of Stress Resistance and Cell Death in S. cerevisiae.
    Valter Longo
    Biological Sciences, University of Southern Califor, 3715 McClintock, Los Angeles, CA 90089, USA (vlongo@usc.edu)

  • 18-12
    •   S. cerevisiae : A system to express and analyse human G6PD variants.
    Dorota Grabowska (1), Ewa Jablonska-Skwiecinska (2), Anna Chelstowska (1), Irmina Lewandowska (1), Danuta Plochocka (1), Beata Burzynska (1)
    (1) Department of Genetics, Institute of Biochemistry and Pawinskiego 5A, Warszawa, 02-106, Poland (grab@ibb.waw.pl); (2) Department of Laboratory Diagnostics, Medical Centre of Postgraduate Education, Banacha 1a, 02-097 Warsaw, Poland

  • 18-13
    •   Expression in an msb3msb4 yeast mutant of 2 human homologues of the yeast MSB3 MSB4 genes: RN-tre and Tre2 oncogene.
    Christelle Bizimungu, Arsène Burny, Daniel Portetelle, Micheline Vandenbol
    Unité de Microbiologie, FUSAGx, Av. Maréchal Juin, 6, Gembloux, B-5030, Belgium (bizimungu.c@fsagx.ac.be)

  • 18-14
    •   Saccharomyces cerevisiae as a model system for the study of the regulation of the mammalian AMP-activated protein kinase (AMPK).
    Jose V. Gimeno-Alcañiz, Pascual Sanz
    Insto. Biomedicina de Valencia, CSIC, Jaime Roig 11, Valencia, E-46010, Spain (jgimeno@ibv.csic.es)

  • 18-15
    •   Programmed cell death in the pathogenic yeast Candida albicans.
    Andrew Phillips, Jon Crowe, Alistair Brown, Mark Ramsdale
    Molecular & Cell Biology, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom (M.Ramsdale@abdn.ac.uk)

  • 18-16
    •   The mechanisms of [URE3] elimination demonstrate that it is not an insoluble problem for the yeast cells.
    Leslie Ripaud, Laurent Maillet, Christophe Cullin
    IBGC, CNRS, 1 rue Saint-Saens, Bordeaux, 33077, France (ripaud@ibgc.u-bordeaux2.fr)

  • 18-17
    •   Conservation of [URE3] through the evolution in the hemiascomycete phylum.
    Nicolas Talarek, Michel Aigle, Christophe Cullin
    IBGC, CNRS, 1 rue C. Saint-Saëns, Bordeaux, 33077, France (talarek@ibgc.u-bordeaux2.fr)

  • 18-18
    •   Using yeast to reveal the function of the mammalian pro-apoptotic BAX molecule.
    Gal Haimovich, Atan Gross
    Biological Regulation, Weizmann Institute of Science, Herzel st., Rehovot, 76100, Israel (gal.haimovich@weizmann.ac.il)

  • 18-19
    •   Selection of longlived mutants of S. cerevisiae.
    Stefanie Jarolim (1), Gino Heeren (1), Peter Laun (1), Alena Pichova (2), Breitenbach Michael (1)
    (1) Department of Genetics, University of Salzburg, Hellbrunnerstrasse 3, Salzburg, 5020, Austria (stefanie.jarolim@sbg.ac.at); (2) Inst. of Microbiology, Czech Academy of Sciences, Prague, Czech Republic

  • 18-20
    •   Effect of mild stress on survival in stationary phase in Saccharomyces cerevisiae.
    Nadège Minois, Magdalena Frajnt, James Vaupel
    Demographic Research, Max Planck Institute, Konrad-Zuse str 1, Rostock, 18057, Germany (frajnt@demogr.mpg.de)

  • 18-21
    •   Loss of the deubiquitinating enzyme Ubp10 induces oxidative stress and apoptosis in budding yeast.
    Maurizio Bettiga, Ivan Orlandi, Lilia Alberghina, Marina Vai
    Biotecnologie e Bioscienze, Università di Milano-Bicocca, P.za della Scienza 2, Milano, 20126, Italy (maurizio.bettiga@unimib.it)

  • 18-22
    •   Synthesis of Triacylglycerols Is Required for the Fission Yeast Schizosaccharomyces pombe to Enter Stationary Phase.
    Qian Zhang, Hai Kee Chieu, Choonpei Low, Shaochong Zhang, Hongyuan Yang
    Biochemistry, National Univ. of Singapore, 10 Kent Ridge Cresce, Singapore, 119260, Republic of Singapore (bchyangr@nus.edu.sg)

  • 18-23
    •   A truncated form of KlLsm4p, a component of the Lsm complex leads to enhanced mRNA stability and apoptosis in yeast.
    Cristina Mazzoni (1), Agnese Serafini (1), Frank Madeo (2), Claudio Falcone (1)
    (1) Department of Cell and Dev, University of Rome 'La Sapienz, Piazzale Aldo Moro 5, Rome, 00185, Italy (cristina.mazzoni@uniroma1.it); (2) Physiologish-chemisches Institut, Universität Tübingen, 72076 Tübingen, Germany

  • 18-24
    •   Actin Dynamics Modulate Cell Survival.
    Campbell Gourlay, Lindsay Carpp, Paul Timpson, Kathryn Ayscough
    IBLS, Department of B and MB, Glasgow University, University Ave., Glasgow, G12 8QQ, UK (cwg2e@udcf.gla.ac.uk)

  • 18-25
    •   Deubiquitinating enzymes in the pathogenesis of neurodegenerative diseases: the view from the yeast system.
    Tatiana A. Chernova (1), Kim D. Allen (2), John R. Shanks (1), Yury O. Chernoff (2), Keith D. Wilkinson (1)
    (1) Department of Biochemistry, Emory University, 1510 Clifton Rd., Atlanta, GA 30322, USA (tcherno@emory.edu); (2) School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 225 North Av., Atlanta, GA 30332, USA

  • 18-26
    •   Cellular control of prion formation in yeast: role of Hsps, endocytic pathway and ubiquitin system.
    Yury O. Chernoff, Kim D. Allen, Kavita P. Bapat, Gary P. Newnam, Laura Ozolins, Esther Tennant-Clegg, Renee D. Wegrzyn
    School of Biology and IBB, Georgia Inst. of Technology, 310 Ferst Drive, Atlanta, GA 30332-0230, USA (yc22@prism.gatech.edu)

  • 18-27
    •   Efficient killer toxin-mediated cell killing requires yeast caspase 1 and apoptosis.
    Jochen Reiter, Manfred J. Schmitt
    Angewandte Molekularbiologie, Universitaet des Saarlandes, Am Stadtwald, Saarbruecken, 66041, Germany (jr@microbiol.uni-sb.de)

  • 18-28
    •   Search for the [ ISP + ] prion-like determinant host gene using the yeast insertion library.
    Kirill Volkov, Anna Aksenova, Sofia Rodionova, Ludmila Mironova
    Dept. of Genetics, St. Petersburg University, Universitetskaya 7/9, St. Petersburg, 199034, Russia (mila@lm2016.spb.edu)

  • 18-29
    •   Oxidative stress and mother cell-specific aging in Saccharomyces cerevisiae.
    Gino Heeren (1), Peter Laun (1), Stefanie Jarolim (1), Gabriel Perrone (2), Nazif Alic (2), Ian Dawes (2), Michael Breitenbach (1)
    (1) Genetics and General Biology, University of Salzburg, HelbrunnerstraBe, Salzburg, 5020, Austria (gino.heeren@sbg.ac.at); (2) School of Biochemistry and Molecular biology, UNSW, Sydney, Australia

  • 18-30
    •   Lethality of Escherichia coli induced by polymerization of the N-terminal region of a Saccharomyces cerevisiae prion-like factor, PSI, and isolation of mutants that escaped this lethality.
    Hiroshi Kawaminami, Bun-ichiro Ono
    Biotechnology, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, 525-8577, Japan (rb002979@se.ritsumei.ac.jp)

  • 18-31
    •   Yeast lacking the SOP1/SRO7 encoded tumor suppressor homologue shows increased susceptibility to apoptosis-like cell death on exposure to NaCl stress.
    Ingrid Wadskog (1), Corinna Maldener (2), Frank Madeo (2), Lennart Adler (1)
    (1) Cell and Molecular biology, Göteborg University, Box 462, Göteborg, 405 30, Sweden (ingrid.wadskog@gmm.gu.se); (2) Institute of Physiological Chemistry, University of Tubingen, Hoppe-Seyler Strasse 4, 720 76 Tubingen Germany

  • 18-32
    •   Is the premature aging of RAS2 Val19 cells a result of defects in oxidative management?
    Lydie Hlavatá (1), Hugo Aguilaniu (2), Alena Pichová (1), Thomas Nystrom (2)
    (1) Institute of Microbiology, Czech Academy od Sciences, Videnska 1083, Prague 4, 142 20, Czech Republic (lydie.hlavata@gmx.net); (2) Department of Cell and Molecular Biology, Göteborg University, Sweden

  • 18-33
    •   Differential pattern of oxidatively damaged proteins in young and old cells of Saccharomyces cerevisiae.
    Nika Erjavec, Thomas Nyström
    Microbiology, Cell and Molecular Biology, Medicinaregatan 9c, Göteborg, 40530, Sweden (nika.erjavec@gmm.gu.se)

  • 18-34
    •   Oxygen starvation during xylose fermentation induces cell death in Saccharomyces cerevisiae.
    Ricardo Cordero Otero
    Institute for Wine Biotechnol., Stellenbosch University, Victoria St., Stellenbosch, 7602, South Africa (rrco@sun.ac.za)

  • 18-35
    •   The S. cerevisiae HtrA-like protein, Nma111p, a nuclear protein that interacts with the nuclear pore complex, promotes cell death.
    Birthe Fahrenkrog, Ursula Sauder, Ueli Aebi
    M.E. Müller Institute, Biozentrum, Klingelbergstr. 70, Basel, 4056, Switzerland (birthe.fahrenkrog@unibas.ch)

  • 18-36
    •   Sugar-induced Apoptosis in Yeast Cells.
    David Granot, Edan Dor-Hefetz
    Institute of Field Crops, The Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel (granot@agri.huji.ac.il)

  • 18-37
    •   Prion Domains: Structure and Function.
    Lev Osherovich (1), Angela Dunn (2), Mick Tuite (2), Jonathan Weissman (1)
    (1) Cellular and Mol. Pharmacology, UCSF and HHMI, 600 16th St., San Francisco, CA 94143-2240, USA (lxoshe@itsa.ucsf.edu); (2) Dept. of Biosciences, University of Canterbury, Kent, UK

  • 18-38
    •   A novel cell cycle G2/M regulatory pathway modulated by HIV-1 viral protein R (Vpr) in fission yeast.
    Yuqi Zhao, Robert Elder, Zsigmond Benko, Min Yu, Dong Liang, Karen Chiu, Kefeng Qin
    Feinberg School of Medicine, Northwestern University, 2430 N Halsted St., Chicago, IL 60614, USA (yzhao@northwestern.edu)

  • 18-39
    •   Asymmetric inheritance of oxidatively damaged proteins during cytokinesis.
    Hugo Aguilaniu (1), Lena Gustafsson (1), Michel Rigoulet (2), Thomas Nyström (3)
    (1) Molecular Biotechnology, Chalmers University, Box 462, Göteborg, 40530, Sweden (hugo.aguilaniu@molbiotech.chalmers.se); (2) Institut de Biochimie et de Génétiques Cellulaires du CNRS, Université Victor Segalen, Bordeaux, France; (3) Department of Cell and Molecular Biology - Microbiology, Göteborg University, Göteborg, Sweden

  • 18-40
    •   Mitochondrial Biogenesis, Aging and Oxidative Stress in Saccharomyces cerevisiae: Investigation on the Function of Uth1p.
    Ingrid Kissova, Gisele Velours, Stephen Manon, Nadine Camougrand
    UMR5095, CNRS, 1 rue Camille StSaëns, Bordeaux, 33077, France (n.camougrand@ibgc.u-bordeaux2.fr)

  • 18-41
    •   Contributions to the physiology, the genetics and genomics of yeast mother cell-specific aging.
    Michael Breitenbach (1), Peter Laun (1), Gino Heeren (1), Stefanie Jarolim (1), Alena Pichova (2), Frank Madeo (3)
    (1) Dept. of Genetics, University of Salzburg, Hellbrunnerstr. 34, Salzburg, A-5020, Austria (Michael.Breitenbach@sbg.ac.at); (2) Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic; (3) Department of Physiological Chemistry, University of Tübingen, Hoppe-Seyler-Str. 4, D-72076 Tübingen, Germany

  • 18-42
    •   Oxygen concentration and ascorbate modulate life span and some other physiological consequences of superoxide dismutase (CuZnSOD) deficiency in yeast.
    Tomasz Bilinski (1), Jaroslaw Wawryn (2), Anna Krzepilko (2), Agata Swiecilo (2), Renata Zadrag (1), Ewa Zyracka (1), Grzegorz Bartosz (1)
    (1) Biochemistry, University of Rzeszow, Rejtana 16, Rzeszow, 35-605, Poland (bilinski@univ.rzeszow.pl); (2) Zamosc College of Agriculture, Agricultural University of Lublin, Szczebrzeska 102 Zamosc, 22-400 Poland

  • 18-43
    •   Analysis of the cell death induced by different values of Double-strand DNA breaks in Saccharomyces cerevisiae.
    Aouida Mustapha (1), Tounekti Omar (2), Belhadj Omrane (3), Mir Lluis (1)
    (1) Laboratoire Vectorologie et Transfert de Gènes, UMR 8121 CNRS, Institut Gustave-Roussy, 39 rue Camille Desmoulins, 94805 Villejuif Cédex, France; (2) The Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital 3755 Chemin Cote Ste-Catherine, Montreal, QC, H3T 1E2, Canada.; (3) Laboratoire de Biochimie et Technobiologie, Faculté des Sciences de Tunis, Campus universitaire, 1060 Tunis, Tunisie (maouida@webmails.com)

  • 18-44
    •   Search for substrates of Cdk5 kinase and factors that regulate Cdk5-p35 activity using the yeast Saccharomyces cerevisiae.
    Youko Horiuchi (1), Akio Toh-e (2), Shin-ichi Hisanaga (3), Masafumi Nishizawa (1)
    (1) Dept. Microbiology, Keio University Medical School, Shinjuku, Tokyo, 160-8582, Japan (youko-h@jk9.so-net.ne.jp); (2) Dept. Biological Sciences, The University of Tokyo Graduate School of Science, Bunkyo, Tokyo, Japan; (3) Dept. Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Tokyo, Japan

  • 18-45
    •   Functional relationship between the Saccharomyces cerevisiae Hsp40 Ydj1 and the human homologue Hdj-2.
    Ilaria Malanchi (1), Maria Scarselli (2), Michela Bernardini (2), Neri Niccolai (2), Cesira L. Galeotti (1)
    (1) Antimicrobial and Biocomputing, IRIS, Chiron Srl, Via Fiorentina 1, Siena, 53100, Italy (cesira_galeotti@chiron.it); (2) Department of Molecular Biology, University of Siena, Via Fiorentina 1, 53100 Siena, Italy

  • 18-46
    •   Age-related hotspots of oxidative damage in yeast genomic and mitochondrial DNA.
    Lynne Cheng (1), Damian Parry (1), Peter Strike (2), Lesley Iwanejko (1)
    (1) School of Biological Sciences, University of Liverpool, Crown Street, Liverpool, L69 7ZB, United Kingdom (iwanejko@liv.ac.uk); (2) Faculty of Health and Life Sciences, Napier University, 74 Canaan Lane, Edinburgh, EH10 4TB UK.

  • 18-47
    •   Mechanism of elimination of the yeast [PSI +] prion by overexpression of the molecular chaperone Hsp104.
    Frederique NESS, Brian S COX, Mick F TUITE
    Department of Biosciences, University of Kent, Gile Lane, Canterbury, CT2 7NJ, UK (F.Ness@ukc.ac.uk)

  • 18-48
    •   Receptor tyrosine kinase assay system in yeast.
    Tea Gunde, Alcide Barberis
    University of Zurich, ESBATech AG, Wagistrasse 21, Schlieren, 8952, Switzerland (gunde@esbatech.com)

  • 18-49
    •   Post-Translational Activation of SOD1 Regulated by Oxygen and CCS.
    Nina Brown, Andrew Torres, Yoshiaki Furukawa, Thomas O'Halloran
    Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, U.S.A. (nmbrown@chem.northwestern.edu)

  • 18-50
    •   High sugar concentrations trigger Saccharomyces cerevisiae into a programmed cell death process.
    Roberto Sotoca (1), Rui Silva (2), Paula Ludovico (3), Filipe Sansonetty (3), José Martinez-Peinado (1), Manuela Côrte-Real (2)
    (1) Department of Microbiology, Faculty of Biology, Universidad Complutense, 28040 Madrid, Spain; (2) Departamento de Biologia-Centro de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal (mcortereal@bio.uminho.pt); (3) Escola de Ciências da Saúde, Instituto de Ciências da Vida e da Saúde, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal

  • 18-51
    •   A new two-hybrid technique for screening of cytochrome C-protein interactions.
    Björn Johansson, Manuela Corte-Real
    Dept. of Biology, CCAB, Campus de Gualtar, Braga, 4710-057, Portugal (bjorn_johansson@bio.uminho.pt)

    Poster Session 19 - New concepts in yeast biotechnology

  • 19-1
    •   A cellular system for positive selection of beta-secretase inhibitors.
    Oliver Middendorp, Urs Luethi, Alcide Barberis
    ESBATech AG, Protease Unit, Wagistr. 21, Zürich-Schlieren, 8952, Switzerland (middendorp@esbatech.com)

  • 19-2
    •   Study of protein and gene expression during fermentation and in response to stress using 2D and Microarrays technologies.
    Dominique Kobi, Laurence Gutfreund
    TEPRAL research center, Brasseries Kronenbourg, Oberhausbergen, Strasbourg, 67037, France (dominique.kobi@tepral-fr.com)

  • 19-3
    •   Differentiation of yeast isolates from Polish brewery by PCR-RFLP of rDNA.
    Wojciech Barszczewski (1), Malgorzata Robak (2)
    (1) Kat. Biotechnologii i Mikrobiologii Zywnosci, AR Wroclaw, Norwida 25, 50-375 Wroclaw, Poland (barszcz3@wp.pl); (2) (mrob@ozi.ar.wroc.pl)

  • 19-4
    •   A role for aquaporins in yeast freeze tolerance revealed by genome-wide gene expression analysis.
    An Tanghe (1), Patrick Van Dijck (1), Aloys Teunissen (2), Stefan Hohmann (3), Johan Thevelein (1)
    (1) Lab. of Molecular Cell Biology, Katholieke Universiteit Leuven, Kasteelp. Arenb. 31, Heverlee, 3001, Belgium (An.Tanghe@bio.kuleuven.ac.be); (2) Department of Pharmacology, ErasmusMC, Box 1738, 3000 DR-Rotterdam, The Netherlands; (3) Department of Cell and Molecular Biology, Göteborg University, Box 462, S-40530 Göteborg, Sweden.

  • 19-5
    •   Mouse polyomavirus large T antigen inhibits growth of Saccharomyces cerevisiae cell and alters cell and colony morphology.
    Tomas Adamec, Zdena Palkova, Jitka Forstova
    Dpt. Genetics and Microbiology, Charles University, Vinicna 5, Prague 2, 128 44, Czech Republic (tomged@natur.cuni.cz)

  • 19-6
    •   Protein profile of yeasts under cold stress.
    Sonia Rodríguez-Vargas (1), Anders Blomberg (2), Francisca Randez-Gil (1)
    (1) Biotechnology, IATA (CSIC), Box. 73, Burjassot, 46100, Valencia, Spain (soniarv@iata.csic.es); (2) Department of Cell and Molecular Biology, Göteborg University, Box462, S-40530 Göteborg, Sweden

  • 19-7
    •   Ag+ specifically affects heterologously expressed Kir2.1 in Saccharomyces cerevisiae.
    Guido Hasenbrink, Jost Ludwig, Hella Lichtenberg-Fraté
    AG Molekulare Bioenergetik, Universität Bonn, Kirschallee 1, Bonn, 51335, Germany (G_Hasenbrink@gmx.de)

  • 19-8
    •   Production of Arthromyces ramosus peroxidase by three eukaryotic expression hosts: Aspergillus awamori is the preferred host compared to Saccharomyces cerevisiae and Pichia pastoris.
    B. Christien Lokman (1), Vivi Joosten (1), Robin J. Gouka (2), C. Theo Verrips (2), Cees A.M.J.J. van den Hondel (1)
    (1) Appl Microbiol and GeneTechnol, Nutrition and Food Research, P.O. Box 360, Zeist, 3700 AJ, The Netherlands (lokman@voeding.tno.nl); (2) Unilever Research Vlaardingen, P.O. Box 114, 3130 AC Vlaardingen, The Netherlands

  • 19-9
    •   Genetic and biochemical stability of wine yeast.
    Alina Kunicka, Katarzyna Rajkowska
    Inst. Ferm. Techn. & Microbiol, Technical University of Lodz, Wolczanska 171/173, Lodz, 90-924, Poland (akunicka@ck-sg.p.lodz.pl)

  • 19-10
    •   Adaptation of aprotinin producing Saccharomyces cerevisiae strains to glucose limited conditions.
    Astrid Mørkeberg (1), Kirsten Shin Nielsen (1), Ivan Diers (2), Lars Højlund Christensen (2), Kasper Møller (1), Lisbeth Olsson (1)
    (1) Center for Process Biotechnology, Technical University of Denmark, Building 223, DK-2800 Lyngby (ast@biocentrum.dtu.dk); (2) Novo Nordisk A/S, Bagsværd, DK-2880 Bagsværd

  • 19-11
    •   A cell growth selection system to detect extracellular and transmembrane protein interactions.
    David Urech, Peter Lichtlen, Alcide Barberis
    Technology, ESBATech AG, Wagistr. 21, Zuerich-Schlieren, 8952, Switzerland (urech@esbatech.com)

  • 19-12
    •   New concepts in wine yeast biotechnology.
    Isak Pretorius
    Director of Research, Australian Wine Research Inst, Waite Road, Urrbrae, Adelaide, SA 5064, Australia (Sakkie.Pretorius@awri.com.au)

  • 19-13
    •   A modular system for adding multiple epitope tags to recombinant proteins in Yarrowia lipolytica.
    Christof Gysler, Lars Pampel, Olivia Morandi, Sophie Meier, Ingrid Klenner
    Bioscience, Nestle Research Centre, Vers-chez-les-blanc, Lausanne 26, CH-1000, Switzerland (christof.gysler@rdls.nestle.com)

  • 19-14
    •   Verification of a high-sugar liquid dough model system and its application to the genome-wide expression analysis of industrial baker's yeast strains.
    Joaquin Panadero, Francisca Randez-Gil, Jose Antonio Prieto
    Biotechnology, Ito. Agroquímica (CSIC), P.O. Box 73, Burjassot, 46100, Valencia, Spain (btcpar@iata.csic.es)

  • 19-15
    •   GLP-1 engineering in yeast for the Type 2 diabetes treatment.
    Michi Egel-Mitani (1), Per Franklin Nielsen (2)
    (1) Biotechnology, Novo Nordisk A/S, Novo Alle, Bagsvaerd, 2880, Denmark (mem@novonordisk.com); (2) Protein Science, Novo Nordisk A/S, Novo Alle, 2880 Bagsvaerd, Denmark

  • 19-16
    •   Expression of stress response genes along a simulation of the industrial process of yeast biomass production.
    Roberto Pérez-Torrado (1), Crister Larson (2), Lena Gustafsson (2), Emilia Matallana (1)
    (1) Bioquímica y Biol. Molecular, Universitat de Valencia, Polígono La Coma s/n, Paterna, Valencia, 46980, Spain (rober@iata.csic.es); (2) Departament of Molecular Biotechnology, Lundberg Laboratory, Chalmers University of Technology, Box 462, SE-40530 Göteborg, Sweden

  • 19-17
    •   Production of the recombinant human fibroblast interferon in Pichia pastoris.
    Lyubov Parfenova, Marina V. Padkina
    Biochemistry, Institute of Biology, SPbSU, Oranienbaumskoe sh.2, St-Petersburg, 198904, Russia (lyuba_par@mail.ru)

  • 19-18
    •   Genome-wide Analysis of yeast gene expression during wine fermentation.
    Tristan Rossignol (1), Laurent Dulau (2), Bruno Blondin (1)
    (1) UMR SPO, INRA, 2 place viala, Montpellier, 34060, France (rossignt@ensam.inra.fr); (2) Lallemand SA, Toulouse, France

  • 19-19
    •   Prolonged chemostat cultivation of Saccharomyces cerevisiae under glucose limitation leads to a partial loss of fermentative capacity.
    Mickel Jansen (1), Adham Hassane (1), Jack Pronk (1), Han de Winde (2)
    (1) Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands; (2) DSM Life Sciences, Bakery Ingredients Division, Technology Cluster, PO Box 1, 2600 MA Delft, The Netherlands (j.h.dewinde@tnw.tudelft.nl)

  • 19-20
    •   Maltose hypersensitivity of Saccharomyces cerevisiae as a result of prolonged chemostat cultivation under maltose limitation.
    Mickel Jansen (1), Jack Pronk (1), Han de Winde (2)
    (1) Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands; (2) DSM Life Sciences, Bakery Ingredients Division, Technology Cluster, PO Box 1, 2600 MA Delft, The Netherlands (j.h.dewinde@tnw.tudelft.nl)

  • 19-21
    •   Genomics and biochemical analyses reveal functional adaptation of hexose transport in industrial strains of baker's yeast.
    Han de Winde (1), Gabby Krens (1), Coen van der Weijden (2), Barbara Bakker (2), Thomas Petit (1)
    (1) DSM Life Sciences, Bakery Ingredients Division, Technology Cluster, PO Box 1, 2600 MA Delft, The Netherlands (j.h.dewinde@tnw.tudelft.nl); (2) Molecular Cell Physiology, Free University Amsterdam, De Boelelaan 1083, 1081 HV, Amsterdam, The Netherlands

  • 19-22
    •   Hepatitis B surface antigene (HBsAg) expression system based on the glucose repression deficient mutants of the methylotrophic yeast Hansenula polymorpha.
    Andrei A. Sibirny (1), Olena S. Krasovska (1), Oleh V. Stasyk (1), Vitali A. Kordium (2), Oleksandr F. Vozianov (3)
    (1) Molecular Genetics Biotechnol., Institute of Cell Biology, Drahomanov St. 14/16, Lviv, 79005, Ukraine (sibirny@biochem.lviv.ua); (2) Institute of Molecular Biology and Genetics, Zabolotny Str. 150, Kyiv 03627, Ukraine; (3) Ukrainian Academy of Medical Sciences, Gertsen Str. 12, Kyiv 04050, Ukraine

  • 19-23
    •   Factors that affect the consumption of fructose by Saccharomyces cerevisiae wine strains.
    Nele J. Berthels (1), Ricardo R. Cordero Otero (2), Florian F. Bauer (2), Johan M. Thevelein (1), Isak S. Pretorius (3)
    (1) Lab for Molecular Cell Biology, K.U.Leuven and V.I.B., Kasteelpark Arenberg, Heverlee-Leuven, B-3001, Flanders, Belgium (iwbt29@sun.ac.za); (2) Institute for Wine Biotechnology, Stellenbosch University, Private Bag X1, 7602 Stellenbosch, South Africa; (3) The Australian Wine Research Institute, Waite Road, Urrbrae Glen Osmond, Adelaide SA 5064, Australia

  • 19-24
    •   Analysis of variables that influence methanol synthesis in tequila production (stages: cooking and fermentation).
    Patricia Tellez (1), Miguel Cedeño (2), Humberto Gutiérrez (3), Jaime Alvarez (1)
    (1) Coordinación Académica, CIATEJ, Normalistas 800 SH, Guadalajara, 44270, México (ptellez@ciatej.net.mx); (2) Tequila Herradura, S.A. de C.V., Guadalajara, 44270, México; (3) University of Guadalajara, Guadalajara, 44270, México

  • 19-25
    •   Isolation, selection and influence of yeast on tequila quality.
    Patricia Tellez (1), Alfredo Feria (1), Fernando Peraza (2)
    (1) Coordinacion Académica, CIATEJ, Av Normalistas 800, Guadalajara, 44270, México (ptellez@ciatej.net.mx); (2) ITA 26, km 10 carr. a San Miguel Cuyutlán, Tlajomulco de Zéñiga, Jal.

  • 19-26
    •   Investigating the link between yeast genes and wine flavour.
    Jeff Eglinton, Anthony Heinrich, Alan Pollnitz, Paul Henschke, Miguel de Barros Lopes
    The Australian Wine Research Institute, PO Box 197, Glen Osmond SA 5064, Australia (Jeff.Eglinton@awri.com.au)

  • 19-27
    •   Polygalacturonase production in wine yeast strains and its carbon source regulation.
    Florentina Radoi, Masao Kishida, Haruhiko Kawasaki
    Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka, 599-8531, Japan (masyksd@biochem.osakafu-u.ac.jp)

  • 19-28
    •   Application of exogenous stress factors to higher production of carotenoids by industrial red yeast.
    Radka Koci, Ivana Marova, Ondrej Koutny, Jana Pokorna
    Faculty of Chemistry, Brno Technical University, Purkynova 118, Brno, 612 00, Czech Republic (radkakoci@seznam.cz)

  • 19-29
    •   Influence of flocculation on the ethanol production by recombinant Saccharomyces cerevisiae expressing amylolytic genes from Lipomyces kononenkoae.
    Ricardo Cordero Otero, Nivetha Ramachandran, Sakkie Pretorius
    Institute for Wine Biotechnol., Stellenbosch University, Victoria street, Stellenbosch, 7602, Republic of South Africa (iwbt25@sun.ac.za)

  • 19-30
    •   Molecular genotyping of film-forming wine yeast strains.
    Mónika Kovács, Anna Maráz
    Dep. of Microb. and Biotech., Szent Istvan University, Somloi ut 14-16, Budapest, 1118, Hungary (monika_ranga@yahoo.com)

  • 19-31
    •   Target identification and metabolic engineering driven by genome-wide transcription profiling.
    Jürgen Bauer, Sonja Fiesel, Sandra Bettermann, Sandra Weich, Oliver Sorgenfrei
    Professional Services, Axaron Bioscience AG, INF 515, Heidelberg, 69120, Germany (bauer@axaron.com)

  • 19-32
    •   Genome-wide expression analysis of genes affected by heterologous protein production in fission yeast.
    Hideki Tohda, Hiromichi Kumagai, Yuko Giga-Hama
    ASPEX Division, Asahi Glass Co., Ltd., Kanagawa-ku, Yokohama, 221-8755, Japan (htohda@agc.co.jp)

  • 19-33
    •   Protease-deficient strains of fission yeast Schizosaccharomyces pombe for heterologous protein production.
    Bi Kewei, Hideki Tohda, Hiromichi Kumagai, Yuko Giga-Hama
    ASPEX Division, Asahi Glass Co., Ltd., Kanagawa-ku, Yokohama-shi, 221-8755, Japan (hama@agc.co.jp)

  • 19-34
    •   Role of Gpd1p and of the HOG pathway in glycerol formation during wine fermentation.
    Fabienne Remize, Brigitte Cambon, Sylvie Dequin
    UMR SPO, Microbiologie, INRA, 2, place Viala, Montpellier, 34060, France (dequin@ensam.inra.fr)

  • 19-35
    •   Carbon dioxide and its relevance in Baker's yeast fermentation.
    Thomas Petit, Han de Winde, Andre Terwisscha van Scheltinga, Herve Hartmann
    Bakery Ingredients Division, DSM Life Sciences, PO Box 1, Delft, 2600 MA, The Netherlands (thomas.petit@dsm.com)

  • 19-36
    •   Nitrogen- and oxygen-limited recirculation cultivation for ethanol production by Saccharomyces cerevisiae.
    Tomas Brandberg (1), Lena Gustafsson (2), Carl Johan Franzén (1)
    (1) Chemical Engineering and Environmental Science, Chalmers University of Technology, SE-412 96 Göteborg, Sweden (tbrand@cre.chalmers.se); (2) Chemistry and Bioscience, Molecular Biotechnology, Chalmers University of Technology, Box 462, SE-405 30 Göteborg, Sweden

  • 19-37
    •   Roles of the TCA reductive pathway during yeast alcoholic fermentation.
    Carole Camarasa, Virginie Faucet, Sylvie Dequin
    UMR-Sciences pour l'OEnologie, INRA, 2 Place Viala, Montpellier, 34060, France (camarasa@ensam.inra.fr)

  • 19-38
    •   Cloning and expression of human Enamelin in Saccharomyces cerevisiae.
    Boaz Laadan, Marie Jeppsson, Bärbel Hahn-Hägerdal
    Applied Microbiology, Lund university, Getingevagen 60, Lund, 22100, Sweden (Boaz.Laadan@tmb.lth.se)

  • 19-39
    •   Genetic and enological analyses of a genetically enhanced malolactic wine yeast strain: A new era in wine making.
    Hennie J.J. van Vuuren, George K. van der Merwe, John Husnik
    Wine Research Centre, University of British Columbia, 2205 East Mall, Vancouver, V6T 1Z4, Canada (hjjvv@interchange.ubc.ca)

  • 19-40
    •   Intracellular Expression of Human Calcitonin Gene in Yeast Cells: Potential Application as Functional Food.
    Hamideh Ofoghi (1), Farzin Rouhvand (2)
    (1) Biotechnology, IROST, Forsat, Tehran-15819, 15815/3538, Iran (hamideh2@yahoo.com); (2) Molecular Biology Unit, Pasteur Institute of Iran

  • 19-41
    •   Improvement of Wine Aroma by Overproduction of Isoamyl Acetate in Wine Yeasts.
    Genoveva Uber-García (1), Daniel Ramón (1), Emilia Matallana (2)
    (1) Biotechnology, I.A.T.A(CSIC), Poligono La Coma s/n, Paterna, 46100, Spain (guber@iata.csaic.es); (2) Departamento de Bioquímica y Biología Molecular. Universitat de València, Spain.

  • 19-42
    •   Expression of a single-chain antibody in Kluyveromyces lactis.
    Jorrit-Jan Krijger, Karin D. Breunig
    Molecular Genetics, Institute of Genetics, Weinbergweg 10, Halle/Saale, D-06120, Germany (krijger@genetik.uni-halle.de)

    Poster Session 20 - Beyond yeast: other model organisms studied by yeast researchers

  • 20-1
    •   Genetic transformation of the pathogenic yeast Candida parapsilosis.
    Julia Zemanova (1), Jozef Nosek (1), Lubomir Tomaska (2)
    (1) Department of Biochemistry, Comenius Univ., Fac. Nat. Sci., Mlynska dolina CH-1, Bratislava, 84215, Slovakia (Julia.Zemanova@fns.uniba.sk); (2) Department of Genetics, Comenius Univ., Fac. Nat. Sci., Mlynska dolina B-1, 84215 Bratislava, Slovakia

  • 20-2
    •   Resistance to phleomycin-type antibiotics as a dominant selective marker in Schwanniomyces occidentalis.
    Andriy Dorosh, Jiri Hasek, Ivana Janatova
    Lab. of Cell Reproduction, Institute of Microbiology, Videnska 1083, Prague 4, 14220, Czech Republic (andrei@biomed.cas.cz)

  • 20-3
    •   Cytoplasmic helicase encoded by pGKL plasmids.
    Martina Pejznochova, Vaclav Vopalensky, Martin Pospisek
    Dep. Genetics and Microbiology, Charles University, Vinicna 5, Prague 2, 128 44, Czech Republic (maary@centrum.cz)

  • 20-4
    •   Protein ensemble of yeast linear plasmids.
    Petra Sekyrova, Vaclav Vopalensky, Martin Pospisek
    Dept. Genetics & Microbiology, Charles University, Vinicna 5, Prague 2, 128 44, Czech Republic (dendrobates@centrum.cz)

  • 20-5
    •   Overproduction of proteins with prion-like domains can cure cells of the [ PSI + ] prion.
    Sviatoslav Bagriantsev (1), Joo Hong (1), Irina Derkatch (2), Susan Liebman (1)
    (1) Biological Sciences, University of Illinois, 900 S Ashland, Chicago, IL 60607, USA (sbagri2@uic.edu); (2) New York University, School of Medicine, 550 First Avenue, New York, NY 10016, USA

  • 20-6
    •   Integration mutagenesis in the respiratory yeast Pichia stipitis.
    Barbara Schruff, Nicole Maaßen, Ulrich Klinner
    Microbiology and Genetics, Aachen University, Worringer Weg, Aachen, 52056, Germany (Barbaraschruff@hotmail.com)

  • 20-7
    •   Transcriptional regulation of heme biosynthesis in Kluyveromyces lactis : the HEM1, HEM12 and HEM13 genes.
    Laura Néñez, Moisés Blanco, Manuel Becerra, M. Angeles Freire, M. Isabel González-Siso, M. Esperanza Cerdán
    Dpto. Biol. Cel. Mol., Univ. La Coruña, Campus La Zapateira, La Coruña, 15071, SPAIN (bmanamrt@udc.es)

  • 20-8
    •   High-level production and secretion of recombinant proteins by Arxula adeninivorans.
    Thomas Wartmann (1), Erik Böer (1), Almudena H. Pico (1), Heike Sieber (2), Oliver Bartelsen (2), Gerd Gellissen (2), Gotthard Kunze (1)
    (1) Molekulare Zellbiologie, IPK-Gatersleben, Corrensstr. 3, Gatersleben, D-06466, Germany (kunzeg@ipk-gatersleben.de); (2) Rhein Biotech GmbH, Eichsfelder Str. 11, D-40595 Düsseldorf, Germany

  • 20-9
    •   Genetic suppression of cell cycle G2 arrest induced by HIV-1 viral protein R (Vpr) in fission yeast.
    Zsigmond Benko, Dong Liang, Karen Chiu, Csaba Fenyvesvolgyi, Min Yu, Yuqi Zhao
    Feinberg School of Medicine, Northwestern University, 2430 N Halsted St., Chicago, IL 60614, USA (zbenko@childrensmemorial.org)

  • 20-10
    •   Saccharomyces cerevisiae D7 as a model system for study of antimutagenic effects of plant foods.
    Ivana Marova, Radka Hladikova, Petr Ptacek, Andrea Mikulcova
    Faculty of Chemistry, Brno Technical University, Purkynova 118, Brno, 612 00, Czech Republic (marova@fch.vutbr.cz)

  • 20-11
    •   Glucose regulation of histone acetylation in yeast and mammalian cells.
    Amber Mosley, Sabire Ozcan
    Department of Biochemistry, University of Kentucky, 800 Rose St MN663, Lexington, KY 40517, USA (almosl2@uky.edu)

    Poster Session 21 - Yeast microscopy: how to study the subcellular structure of yeast

  • 21-1
    •   Ultrastructrural Hxt5p localization upon repression of transcription.
    Denise van Suylekom, Rene Verwaal, Bruno Humbel, Johannes Boonstra
    Molecular Cell Biology, Utrecht University, Padualaan 8, Utrecht, 3584 CH, The Netherlands (g.p.vansuylekom@bio.uu.nl)

  • 21-2
    •   Budding yeast Pds5p plays an essential role in meiotic prophase and is required for sister chromatid cohesin.
    Zhaojie Zhang (1), Qun Ren (1), Michael N. Conrad (2), Vincent Guacci (3), Michael E. Dresser (2)
    (1) Zoology and Physiology, University of Wyoming, 16th and Gibbon, Laramie, WY 82071, USA (zzhang@uwyo.edu); (2) Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA; (3) Fox Chase Cancer Center, Basic Science Division, Philadelphia, Pennsylvania 19111, USA

  • 21-3
    •   YPL.db - The Yeast Protein Localization Database.
    Mathias Kals (1), Klaus Natter (1), Georg Habeler (2), Gerhard Thallinger (2), Zlatko Trajanoski (2), Sepp D. Kohlwein (1)
    (1) IMBM-Biochemistry, University Graz, Schubertstr.1, Graz, A8010, Austria (sepp.kohlwein@uni-graz.at); (2) Institute of Medical Engineering, Technical University Graz, Austria

  • 21-4
    •   The spatial organization of lipid synthesis in yeast.
    Klaus Natter (1), Iskandar Dib (1), Peter Leitner (1), Stanley Fields (2), Stephen McCraith (2), Heimo Wolinski (1), Sepp D. Kohlwein (1)
    (1) IMBM-Biochemistry, University of Graz, Schubertstr.1, Graz, A8010, Austria (sepp.kohlwein@uni-graz.at); (2) Howard Hughes Medical Institute, Department of Genetics, University of Washington, Seattle, USA

  • 21-5
    •   Spatial and temporal dynamics of fission deficient mitochondria.
    Stefan Jakobs, Nadia Martini, Alexander Egner, Astrid C. Schauss, Stefan W. Hell
    Dept. NanoBiophotonics, MPI Biophysical Chemistry, Am Fassberg 11, Goettingen, 37077, Germany (sjakobs@gwdg.de)

    Poster Session 22 - Genomics, databases and bioinformatics

  • 22-1
    •   A complete phylogenetic tree of Saccharomyces sensu stricto species based on genotypic microarray analysis.
    Laura C. Edwards (1), Manda E. Gent (2), David C. Hoyle (3), Andrew Hayes (2), Lubomira Stateva (1), Stephen G. Oliver (2)
    (1) Biomolecular Sciences, UMIST, PO Box 88, Manchester, M60 1QD, UK (L.Edwards@postgrad.umist.ac.uk); (2) School of Biological Sciences, University of Manchester, Manchester, M13 9PT, UK; (3) Department of Computer Science, University of Manchester, Manchester, M13 9PL, UK

  • 22-2
    •   Genome-wide analysis of the response to cell wall mutations in the yeast S. cerevisiae.
    Arnaud Lagorce (1), Nicole Hauser (2), Delphine Labourdette (1), Cristina Rodriguez (3), Hélène Martin-Yken (1), Javier Arroyo (3), Jörg Hoheisel (2), Jean François (1)
    (1) INSA / GBA, UMR-CNRS 5504 & INRA 792, 135 av de Rangueil, Toulouse cedex 4, 31400, France (lagorce@insa-tlse.fr); (2) Division of Functional Genome Analysis, DKFZ, 69120 Heidelberg, Germany; (3) Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain

  • 22-3
    •   Transcriptional responses of Saccharomyces cerevisiae to limitations of carbon, nitrogen, phosphorus or sulfur.
    Viktor Boer, Johannes de Winde, Jack Pronk, Matthew Piper
    Department of Biotechnology, Technical University of Delft, Julianalaan 67, Delft, 2628 BC, The Netherlands (v.m.boer@tnw.tudelft.nl)

  • 22-4
    •   A DNA microarray for genome-wide gene expression analysis in fission yeast.
    Yongtao Xue (1), Stefan Haas (2), Laurent Brino (3), Arief Gusnanto, Mark Riemers (4), Driss Talibi (3), Martin Vingron (2), Karl Ekwall (1), Anthony P H Wright (1)
    (1) Natural Science Section, Södertörns University College, Box 4101, Huddinge, S-141 04, Sweden (yongtao.xue@sh.se); (2) Department of Computational Molecular Biology, Max-Plank Institute for Molecular Genetics, Ihnestrasse, D-14195 Berlin, Germany; (3) Eurogentec SA, Parc Scientifique du Sart Tilman, 4102 Seraing, Belgium; (4) Center for Genomics and Bioinformatics, Karolinska Institute, S-171 77, Stockholm, Sweden

  • 22-5
    •   On track to provide convenient genome data: The MIPS Comprehensive Yeast Genome Database.
    Ulrich Gueldener (1), Martin Muensterkoetter (2), Volker Stuempflen (2), Gabi Kastenmueller (2), Hans-Werner Mewes (2)
    (1) (u.gueldener@gsf.de); (2) Institute for Bioinformatics, GSF, Ingolstaedter Landst, Neuherberg, 85764, Germany

  • 22-6
    •   Genomic Run-On: a method to evaluate transcription rates and mRNA half-lives for all yeast genes.
    José García-Martínez, Agustín Aranda, José E. Pérez-Ortín
    Bioquímica y Biol. Molecular, Universitat de València, Dr. Moliner 50, Burjassot, E46100, Spain (jose.e.perez@uv.es)

  • 22-7
    •   Constructing a collection of Tet-promoter alleles for all essential genes in S. cerevisiae.
    Sanie Mnaimneh, Armaity Davierwala, Wen Zhang, Stuart Yang, Jeff Pootoolal, Timothy Hughes
    Banting and Best Medical Res., University of Toronto, 112 College Street, Toronto, ON M5G 1L6, Canada (sanie.mnaimneh@utoronto.ca)

  • 22-8
    •   Metabolic footprinting: a high-throughput, high-information approach to cellular characterisation and functional genomics.
    Douglas B. Kell (1), Jess K. Allen (2), Hazel M. Davey (2), David I. Broadhurst (2), Jem J. Rowland (3), Stephen G. Oliver (4)
    (1) Chemistry, UMIST, Sackville St, Manchester, M60 1QD, UK (dbk@umist.ac.uk); (2) Institute of Biological Sciences, Cledwyn Building, University of Wales, Aberystwyth, Aberystwyth SY23 3DD, UK; (3) Dept of Computer Science, University of Wales, Aberystwyth, Aberystwyth SY23 3DB, UK; (4) School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Rd, Manchester M13 9PT, UK

  • 22-9
    •   Proteomic analysis of the Gcn4-mediated response in Saccharomyces cerevisiae and Candida albicans.
    Z. Yin (1), D. Stead (1), L. Selway (1), J. Walker (1), G. Tripathi (1), T. Mclnerney (2), P. Cash (3), A.J.P. Brown (1)
    (1) Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK (z.yin@abdn.ac.uk); (2) Nonlinear Dynamics Ltd, Tyne House, 26 Side, Newcastle upon Tyne NE1 3JA, UK; (3) Aberdeen Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK

  • 22-10
    •   Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and prediction.
    Hyun Kim, Karin Melén, Gunnar von Heijne
    Biochemistry and Biophysics, Stockholm University, Svante Arrhenius Lab, Stockholm, SE 10609, Sweden (hkim@dbb.su.se)

  • 22-11
    •   Influence of growth rate on the patterns of gene expression and metabolic profiles in yeast chemostat cultures.
    Juan I Castrillo (1), Andrew Hayes (1), David J Gardner (1), June Petty (1), Simon J Gaskell (2), Stephen G Oliver (1)
    (1) School of Biological Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, United Kingdom (Juan.I.Castrillo@man.ac.uk); (2) Michael Barber Centre for Mass Spectrometry, Department of Chemistry, UMIST, Manchester, M60 1QD, UK

  • 22-12
    •   Analysis of diversity in unicellular fungi.
    Ian N. Roberts, Stephen A. James
    NCYC, Institute of Food Research, Colney, Norwich, NR4 7UA, UK (ian.roberts@bbsrc.ac.uk)

  • 22-13
    •   Comparison of gene expression and regulation in budding and fission yeasts.
    Katja Kivinen, Alvis Brazma
    Microarray Informatics, EBI, Hinxton, Cambridge, CB10 1SD, United Kingdom (kivinen@ebi.ac.uk)

  • 22-14
    •   Assessing contributions to fitness of individual genes via genome-wide competition analysis.
    Daniela Delneri (1), Hui Sun Leong (1), Andrew Hayes (1), Hazel M Davey (2), Doug B Kell (3), Stephen G Oliver (1)
    (1) School of Biological Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK (ddelneri@man.ac.uk); (2) Cledwyn Building, Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DD, UK; (3) Department of Chemistry, UMIST, PO Box 88, Sackville St, Manchester M60 1QD, UK.

  • 22-15
    •   High-throughput transformation of multiple yeast deletion strains and its use for genome-wide suppressor analysis.
    Hiroshi Yamamura, Hirotake Fujiwara, Kaori Shinagawa, Hisashi Hoshida, Rinji Akada
    Dept. Appl. Chem. & Chem. Eng., Fac. Eng., Yamaguchi Univ., Tokiwadai, Ube, 755-8611, Japan (rinji@yamaguchi-u.ac.jp)

  • 22-16
    •   The Ashbya Genome Database (AGD) provides information about highly accurate annotated fungus genes and their synteny between Ashbya and Saccharomyces.
    Sophie Brachat (1), Leandro Hermida (1), Fred Dietrich (2), S. Voegeli (1), A. Lerch (1), Christa Gates (3), Tom Gaffney (3), Peter Philippsen, and Michael Primig (1)
    (1) Biozentrum, University of Basel, Klingelbergstrasse, Basel, CH-4056, Switzerland (leandro.hermida@unibas.ch); (2) Duke University, Durham, NC USA; (3) Syngenta Corporation, Wilmington, DE USA

  • 22-17
    •   Yeast phenomics on a genome-wide scale.
    Jonas Warringer, Elke Ericson, Luciano Fernandez, Olle Nerman, Anders Blomberg
    Göteborg University, CMB-microbiology, Medicinaregatan 9c, Göteborg, 41390, Sweden (Jonas.warringer@gmm.gu.se)

  • 22-18
    •   Model organisms Saccharomyces cerevisiae and Schizosaccharomyces pombe in the Swiss-Prot database.
    Kati K.M. Laiho, Rolf Apweiler
    Sequence Database Group, EMBL-EBI, Genome Campus, Cambridge, CB101SD, United Kingdom (kati@ebi.ac.uk)

  • 22-19
    •   YETI: Yeast Exploration Tool Integrator.
    Richard J. Orton (1), William I. Sellers (2), Dietlind L. Gerloff (1)
    (1) ICMB, University of Edinburgh, Kings Buildings, Edinburgh, EH9 3JR, UK (orton@bch.ed.ac.uk); (2) Department of Human Sciences, University of Loughborough, Loughborough, LE11 3TU, UK

  • 22-20
    •   Genomic analysis of biofilm formation in Saccharomyces cerevisiae.
    Maria A. Demontis (1), Marilena Budroni (2), Giovanni A. Farris (2), Gerald R. Fink (1)
    (1) Whitehead Institute, 9 Cambridge Center, Cambridge MA 02142, USA (mademo@uniss.it); (2) Di.S.A.A.B.A., Università di Sassari, Viale Italia 39, Sassari, 07100, Italy

  • 22-21
    •   Roles of Sth1 in the RSC complex as shown by a novel algorithm for optimised clustering of large gene expression data sets.
    Birgitte Regenberg (1), Thomas Grotkjær (1), Ole Winther (2), Lars Kai Hansen (2), Steen Holmberg (3), Jens Nielsen (1)
    (1) BioCentrum-DTU, Tech. University of Denmark, Søltofts Plads, Kgs. Lyngby, 2800, Denmark (tg@biocentrum.dtu.dk); (2) Informatics and Mathematical Modelling, Tech. University of Denmark, 2800 Kgs. Lyngby; (3) Institute of Molecular Biology, University of Copenhagen, 1353 Copenhagen K

  • 22-22
    •   GermOnline is a knowledgebase about meiosis and gametogenesis designed, curated and updated by biologists.
    Christa Wiederkehr, Michael Primig
    Bioinformatics, Biozentrum, Klingelbergstrasse, Basel, CH-4056, Switzerland (christa.wiederkehr@unibas.ch)

  • 22-23
    •   The south-Paris yeast structural genomics project.
    S. Quevillon-Cheruel, H. van Tilbeurgh, N. Leulliot, L. Trésaugues, D. Liger, C.-Z. Zhou, A. Poupon, J. Janin
    IBBMC, Université de Paris-sud, bat430, Orsay, 91405, France (sophie.cheruel@egm.u-psud.fr)

  • 22-24
    •   Finding Metabolic Paths Between Pairs of Interacting Proteins.
    Didier Croes, Fabian Couche, Shoshana Wodak, Jacques van Helden
    SCMBB, cp263, Université libre de Bruxelles, Bld. du Triomphe, Bruxelles, 1050, Belgium (didier@scmbb.ulb.ac.be)

  • 22-25
    •   Transcriptional Regulation of Protein Complexes in Yeast.
    Nicolas Simonis (1), Shoshana Wodak (1), Georges Cohen (2), Jacques van Helden (1)
    (1) SCMBB, Universite libre de Bruxelles, bld du Triomphe, Brussels, 1050, Belgium (nicolas@ucmb.ulb.ac.be); (2) Institut Pasteur, Unité d'Expression des Gènes Eucaryotes, Institut Pasteur, 28, rue du Docteur Roux, 75524 Paris Cedex 15. France

  • 22-26
    •   Identification of metabolic modules from gene expression data.
    Joseph Tran, Shoshana Wodak, Jacques van Helden
    SCMBB, universite Libre de Bruxelles, Bld. du Triomphe, Brussels, 1050, Belgium (jtran@ucmb.ulb.ac.be)

  • 22-27
    •   COGEME- Consortium for the Functional Genomics of Microbial Eukaryotes: Facilities For The Functional Analysis of Microbial Genomes.
    Cristina Merlotti (1), Isabel Riba-Garcia (2), Simon J Gaskell (2), Zhikang Yin (3), Alistair Brown (3), Phil Cash (3), Andrew Hayes (1), Nicholas J Talbot (4), Andrew Brass (5), Norman Paton (5), Stephen G Oliver (1)
    (1) School of Biological Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK (merlotti@man.ac.uk); (2) Michael Barber Centre for Mass Spectrometry, Department of Chemistry, UMIST, Manchester, M60 1QD, UK; (3) Institute of Medical Sciences, University of Aberdeen, Foresterhill Aberdeen AB25 2ZD, UK; (4) School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road Exeter EX4 4QG, UK; (5) Department of Computer Science, University of Manchester, Oxford Road Manchester M13 9PL, UK.

  • 22-28
    •   Functional genomics of wine yeast strain during industrial fermentation process at low temperature.
    Gemma Beltran (1), Maite Novo (1), Adilia Dagkesamansakaia (2), Veronique Leberre (3), J.Manuel Guillamón (1), Albert Mas (1), Jean François (3), Nicolas Rozès (1)
    (1) Biochemistry and Biotechnology, Universitat Rovira i Virgili, Ramon y Cajal, 70, Tarragona, 43005, Spain (gbc@fq.urv.es); (2) Transcriptome and Biochips Plateforme of Genopole Midi-Pyrénées, 135 Av. Rangueil, 31077 Toulouse cedex 04, France; (3) Transcriptome and Biochips Plateforme of Genopole Midi-Pyrénées, Centre de Bioingénierie Gilbert Durand, UMD-CNRS 5504, UMR-INRA 792, INSA, 135 Av. Rangueil, 31077 Toulouse cedex 04, France

  • 22-29
    •   Whole genome screen for ldb mutants in Saccharomyces cerevisiae.
    Isaac Corbacho, Isabel Olivero, Luis M. Hernández
    Department of Microbiology, University of Extremadura, Avda. Elvas s/n, Badajoz, 06071, Spain (icorbacho@unex.es)

  • 22-30
    •   A Genomic Analysis of Quiescence and Exit from the Quiescent State in S. cerevisiae.
    Juanita Martinez, Anthony Aragon, Amanda Archuletta, Angelina Rodriguez, Sushmita Roy, Margaret Werner-Washburne
    Department of Biology, University of New Mexico, Redondo, Albuquerque, NM 87131, USA (mjmartin@unm.edu)

  • 22-31
    •   PROPHECY-a database with standardized high precision quantitative phenotypic information of yeast deletion strains.
    Luciano Fco. Fernandez Ricaud (1), Jonas Warringer (1), Elke Erikson (1), Illona Pylvänäinen (2), Olle Nerman (2), Anders Blomberg (1)
    (1) Cell and Molecular Biology, Göteborg University, Medicinaregatan 9C, Göteborg, 405 30, Sweden (gmmlf@gmm.gu.se); (2) Chalmers university of technology, SE-41296, Göteborg, Sweden.

  • 22-32
    •   The yeast strain collection EUROSCARF: A source for useful tools in yeast genetics and molecular biology.
    Matthias Rose (1), Peter Kötter (1), Jörg Hauf (2), Karl-Dieter Entian (1)
    (1) EUROSCARF, Universität Frankfurt, Marie-Curie-Str. 9, Frankfurt / M., 60439, Germany (Rose@em.uni-frankfurt.de); (2) Scientific Research and Development GmbH, Köhlerweg 20, 61440 Oberursel, Germany

  • 22-33
    •   Predicting synthetic lethality from a diverse collection of gene and protein relationships.
    Sharyl L. Wong (1), Lan O. Zhang (1), Debra S. Goldberg (1), Amy H. Tong (2), Guillaume Lesage (3), Marc Vidal (4), Brenda Andrews (2), Howard Bussey (3), Charles Boone (2), Frederick P. Roth (1)
    (1) Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Ave, Boston, MA, 02115 USA (fritz_roth@hms.harvard.edu); (2) Banting and Best Dept. of Medical Research and Dept. of Medical Genetics and Microbiology, University of Toronto, Toronto ON, Canada M5G 1L6; (3) Dept. of Biology, McGill University, Montreal PQ, Canada H3A 1B1 (4) Dept. of Cancer Biology, Dana-Farber Cancer Institute and Dept. of Genetics, Harvard Medical School, Smith 858, 1 Jimmy Fund Way, Boston, MA 02115, USA;

  • 22-34
    •   Global analysis of nutrient control of gene expression in Saccharomyces cerevisiae during growth and starvation.
    Jian Wu (1), Nianshu Zhang (1), Andrew Hayes (1), Kalliope Panoutsopoulou (2), Stephen Oliver (1)
    (1) School of Biological Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK (Jian.Wu@man.ac.uk); (2) Department of Biomolecular Sciences, UMIST, Manchester M60 1QD, UK.

  • 22-35
    •   The Community Annotation system at the Saccharomyces Genome Database (SGD).
    Chandra L. Theesfeld, Stan Dong, Dianna G. Fisk, Rama Balakrishnan, Karen R. Christie, Maria C. Costanzo, Kara Dolinski, Selina S. Dwight, Stacia R. Engel, Jodi E. Hirschman, Eurie L. Hong, Laurie Issel-Tarver, Anand Sethuraman, Gail Binkley, Shuai Weng, David Botstein, and J. Michael Cherry
    Department of Genetics, Stanford University, Stanford, CA 94305-5120, U.S.A. (yeast-curator@genome.stanford.edu)

  • 22-36
    •   Analysis of mRNA export pathways by genome-wide studies of mRNA export mutants.
    Jurgi Camblong, Françoise Stutz
    Dept. of Cell Biology, University of Geneva, 30 Quai E. Ansermet, Geneva, 1211, Switzerland (Jurgi.Camblong@medecine.unige.ch)

  • 22-37
    •   Learning rules for predicting homologues in hemiascomycetous yeasts using Genolevures manually-curated alignments.
    Macha Nikolski (1), Emmanuelle Beyne (1), Pascal Durrens (2), David J. Sherman (1)
    (1) Bioinformatics Group, LaBRI (CNRS UMR 5800), 351 cr. Libération, Talence, F-33405, France (sherman@labri.fr); (2) Centre de Bioinformatique de Bordeaux, 146, rue Leo Saignat, F-33076 Bordeaux Cedex, France

  • 22-38
    •   Does homologous mitotic recombination occur at specific DNA sites in the Saccharomyces cerevisiae genome?
    Valentina Tosato, Carlo V. Bruschi
    Microbiology Group, ICGEB, AREA Science Park, Trieste, I-34012, Italy (tosato@icgeb.org)

  • 22-39
    •   Yarrowia lipolytica consortium -- development of a physical map as a framework for sequencing of the genome.
    David Ogrydziak (1), Gerold Barth (2), Angel Dominguez (3), Claude Gaillardin (4), Stefan Kerscher (5), Jeong-Yoon Kim (6), Richard Rachubinski (7), Rod Wing (8)
    (1) Food Science, University of California, One Shields Avenue, Davis, CA 95616, USA (dmogrydziak@ucdavis.edu); (2) Insitute of Microbiology, Dresden University of Technology, Mommenstr. 13, D-01062 Dresden, Germany; (3) Departmento de Microbiologia y and Genetica, IMB/CSIC, Plaza de los Doctores de la Reina s/n, Universidad de Salamanca, 37007 Salamanca, Spain; (4) Laboratoire de Genetique Moleculaire et Cellulaire, INRA/CNRS/INA-PG, BP 01, F-78850 Thiverval-Grignon, France; (5) Institut fur Biochemie I, Universitatsklinikum Frankfurt am Main, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany; (6) Department of Microbiology, Chungnam National University, Daejon, 305-764, Korea; (7) Department of Cell Biology, University of Alberta, Medical Sciences Building 5-14, Edmonton, Alberta, T6G 2H7, Canada; (8) Clemson University Genomics Institute (CUGI), 100 Jordan Hall, Clemson, SC, 29634-5727 USA

  • 22-40
    •   Tools for S. cerevisiae and S. pombe transcriptomes mining.
    Philippe Marc (1), Gaëlle Lelandais (2), Frédéric Devaux (2), George M. Church (1), Claude Jacq (2)
    (1) Lipper Center for Computational Genetics and Department of Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115, USA (pmarc@genetics.med.harvard.edu); (2) Laboratoire de Génétique Moléculaire, CNRS UMR8541, Ecole Normale Supérieure, 46 Rue d'Ulm, 75005 Paris, France

  • 22-41
    •   Development of Robust Methods for cDNA Microarrays of Neurospora crassa.
    Gilbert Harrison, Gabriel Quinones, Gary Montry, Mary Anne Nelson
    Department of Biology, University of New Mexico, Castetter Hall, 207, Albuquerque, NM 87131, USA (gilh@unm.edu)

  • 22-42
    •   A Virtual System for Global and Dynamic Analysis of Protein Complex Networks.
    Ting-Fang Wang
    Inst. of Biological Chemistry, Academia Sinica, Nankang, Taipei, 11529, Taiwan (tfwang@gate.sinica.edu.tw)

  • 22-43
    •   The fission yeast genome status.
    Valerie Wood
    PSU, Sanger Institute, Welcome Trust Genome Campus, Cambridge, CB101SA, UK (val@sanger.ac.uk)

  • 22-44
    •   Small is beautiful and meaningful: Identification and characterization of Non-Annotated ORFs (NORFs) in S. cerevisiae.
    Munira Basrai, James Kastenmayer, Carole Carter
    Genetics, National Cancer Institute, 8901 Wissonsin Ave, Bethesda, MD 20889, USA

    Poster Session 23 - New developments and miscellaneous

  • 23-1
    •   Secreted Expression of Fungal Chitin Deacetylase into Yeast, Pichia pastoris.
    Binesh Shrestha (1), Willem F. Stevens (1), Françoise Le Hégarat (2)
    (1) Bioprocess Technology/PT, Asian Institute of Technology, Thailand (st018568@ait.ac.th); (2) Institut de Génétique et Microbiologie, Universite Paris-Sud, France.

  • 23-2
    •   Candida albicans biofilms: a transcript profiling approach.
    Susana García Sánchez (1), Sylvie Aubert (1), Jean-Marc Ghigo (2), Christophe d'Enfert (1)
    (1) Fungal Biology & Pathogenicity, Institut Pasteur, 25 Rue Dr. Roux, Paris, 75075, France (sgarcia@pasteur.fr); (2) Genetics of Biofilms

  • 23-3
    •   The impact of replicative cell age on the flocculation and cell surface characteristics in Saccharomyces species.
    Steven C. Davy (1), Gavin Hulse (2), Katherine A. Smart (1)
    (1) Brewing Yeast Research, BMS, Oxford Brookes University, Gipsy Lane, Oxford, OX3 0BP, UK (sdavy@brookes.ac.uk); (2) South African Breweries, 65 Park Lane, Sandown, Sandton, PO Box 782178, Sandton 2146, South Africa.

  • 23-4
    •   Identification of membrane protein interactions using the transactivator-based membrane yeast two-hybrid technology (TAMYTH).
    Safia Thaminy (1), Daniel Auerbach (2), Anthony Arnoldo (1), Igor Stagljar (1)
    (1) I. of Vet. Biochem. & Mol.Bio., University of Zurich, Winterthurerstr. 190, Zurich, 8057, Switzerland (stagljar@vetbio.unizh.ch); (2) Dualsystems Biotech Inc., Zurich, Switzerland

  • 23-5
    •   Cysteine proteinase inhibitors in yeast.
    Katarzyna Gawlik, Wojciech Poreba, Jan Gutowicz
    Department of Physical Chemistry of Microorganisms, Institute of Genetics and Microbiology, Wroclaw University, Przybyszewskiego 63, Wroclaw, 51-148, Poland (katgaw@microb.uni.wroc.pl)

  • 23-6
    •   Development of a more sensitive test system for detecting genotoxins.
    Milena Kirilova, George Miloshev
    Department Molecular Genetics, Institute of Molecular Biology, Acad. G. Bonchev 21, Sofia, 1113, Bulgaria (Kirilova_mimmi@hotmail.com)

  • 23-7
    •   Cyanide-resistant respiration in Debaryomyces hansenii. The AOX gene.
    Alexandra Veiga, João D. Arrabaça
    Centro de Engenharia Biológica, Faculdade de Ciências, Campo Grande, C2, Lisboa, 1749-016, Portugal (aveigabarros@clix.pt)

  • 23-8
    •   Yeast vector systems for high throughput multiplex expression of heterologous proteins.
    Stephane Leclair (1), Rainer Niedenthal (2), Martin Funk (1)
    (1) Research, MediGene AG, Lochhamerstr. 11, Martinsried, D-82152, germany (m.funk@medigene.com); (2) Institut für Physiologische Chemie;Medizinische Hochschule Hannover; Carl-Neuberg Straße 1; D-30625 Hannover, Germany

  • 23-9
    •   New insight into the production and secretion of heterologous proteins from the 'non conventional' yeast Zygosaccharomyces bailii.
    Paola Branduardi, Minoska Valli, Lilia Alberghina, Danilo Porro
    Dip. Biotecnol. e Bioscienze, Università Milano-Bicocca, P.zza della Scienza, Milano, 20126, Italy (paola.branduardi@unimib.it)

  • 23-10
    •   Interaction of Histoplasma capsulatum yeasts with different phagocytic cells of bats, through in situ adherence assays.
    Maria Lucia Taylor (1), Roberto Oswaldo Suárez-Alvarez (1), Armando Pérez (2), Esperanza Duarte-Escalante (1)
    (1) Microbiología-Parasitología, FM, UNAM, Ciudad Universitaria, Mexico DF, 04510, Mexico (emello@servidor.unam.mx); (2) Biología Celular y Tisular, FM, UNAM, Ciudad Universitaria, Mexico DF, 04510, Mexico

  • 23-11
    •   Yeast-like cells at 37°C of the entomopathogen Metarhizium anisopliae isolated from locusts (Schistocerca piceifrons).
    Conchita Toriello (1), Patricia Jiménez (1), Elena Montoya (1), Claudia Cano-Ramírez (1), Armando Pérez (2), Victor Hernández (3)
    (1) Microbiología y Parasitología, UNAM, Facultad de Medicina, Mexico DF, 04510, Mexico (toriello@servidor.unam.mx); (2) Biología Celular y Tisular, Facultad de Medicina, UNAM, Mexico DF, 04510, Mexico; (3) Centro Nacional de Referencia de Control Biológico, DGSV-SAGARPA, Colima 28130, Mexico

  • 23-12
    •   Duplication of a large segment of the right arm of Saccharomyces cerevisiae chromosome II detected by disruption and linkage analysis of ATP3.
    Katsunori Ohnishi (1), Atsuko Nakai (1), Masaharu Takeda (2), Bun-ichiro Ono (1)
    (1) Department of Biotechnology, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, 525-8577, Japan (ono@se.ritsumei.ac.jp); (2) Department of Applied Life Science, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan

  • 23-13
    •   Development of high-efficient chromosome splitting technology towards large-scale chromosome manipulation in Saccharomyces cerevisiae.
    Minetaka Sugiyama, Shigehito Ikushima, Toshimasa Nakazawa, Yoshinobu Kaneko, Satoshi Harashima
    Biotechnology, Osaka University, 2-1 Yamadaoka, Suita-shi, 565-0871, Japan (sugi2@bio.eng.osaka-u.ac.jp)

  • 23-14
    •   The correlation of oxygen and sugar dependent regulation of glycolysis to the biocontrol activity of the yeast Pichia anomala.
    Elisabeth Fredlund, Ulrika Ãdel Druvefors, Volkmar Passoth, Johan Schnürer
    Department of Microbiology, SLU, Box 7025, Uppsala, S-750 07, Sweden (Volkmar.Passoth@mikrob.slu.se)

  • 23-15
    •   Detection of viable fungal contaminants in heat-treated foods by RT-PCR.
    Gianluca Bleve, Lucia Rizzotti, Franco Dellaglio, Sandra Torriani
    Dip. Scientifico e Tecnologico, Università di Verona, strada Le Grazie 15, Verona, 37134, Italy (bleve@sci.univr.it)

  • 23-16
    •   Production of fungal secondary metabolites by sold-state fermentation: molecular strain improvement.
    Javier Barrios-González, Juan C. Leyva, Edgar C. Sierra, Julio C. Brito, Francisco J. Fernandez, Armando Mejia
    Depto. de Biotecnologia, UAM-Iztapalapa, San Rafael Atlixco, Mexico, D.F., 09340, Mexico (jbg@xanum.uam.mx)

  • 23-17
    •   Development of a method to split yeast chromosome using the PI-SceI endonuclease.
    Eriko Tao (1), Minetaka Sugiyama (2), Satoshi Harashima (2), Masafumi Nishizawa (1)
    (1) Dept. Microbiology, Keio University Medical School, Shinjuku, Tokyo, 160-8582, Japan (teriko@sc.itc.keio.ac.jp); (2) Department of Biotechnology, Graduate School of Engineering. Osaka University, Yamadaoka 2-1, Suita-shi, Osaka

  • 23-18
    •   Expression of Na, K-ATPase in P. pastoris. Fe2+-catalysed cleavage of the recombinant enzyme.
    David Strugatsky, Rivka Goldshleger, Eitan Bibi, Steven J.D. Karlish
    Biological Chemistry, Weizmann Institute of Science, Herzel, Rehovot, 76100, Israel (david.strugatsky@weizmann.ac.il)

  • 23-19
    •   An optimized split ubiquitin system for systematic identification and characterization of membrane protein interactions.
    Petr Obrdlik (1), Mohamed El Bakkoury (2), Tanja Hamacher (3), Corinna Cappellaro (3), Binghua Wu (1), Eckhard Boles (3), Bruno André (2), Wolf Bernd Frommer (1)
    (1) ZMBP, Pfanzenphysiologie, Universitaet Tuebingen, Auf der Morgenstelle, Tuebingen, 72076, Germany (petr.obrdlik@zmbp.uni-tuebingen.de); (2) Laboratoire de Physiologie Cellulaire, Universite Libre de Bruxelles, IBMM, Rue des Prof. Jeener et Brachet 10, 6041 Gosseiles, Belgium; (3) Institut für Mikrobiologie, Goethe-Universitaet Frankfurt, Marie-Curie-Str. 9, D-60439 Frankfurt am Main, Germany

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