GENETIC interaction network from large-scale synthetic lethality
screens.
Amy Hin Yan Tong (1), Brenda Andrews (2), Mike Tyers (3),
Charlie Boone (1)
(1) Banting&Best Medical Research, Best Institute, 112 College St,
Toronto, ON M5G 1L6 , Canada;
(2) Dept of Medical Genetics and Microbiology, University of Toronto,
Toronto ON, Canada M5S 1A8;
(3) Program in Molecular Biology and Cancer, Samuel Lunenfeld Research
Institute, Mt. Sinai Hospital, Toronto ON, Canada M5G 1X5
We are applying synthetic genetic array (SGA) methodology for the
systematic construction of double mutants with the set of ~5000 viable
gene deletions [Tong et al., Science 294: 2364-2368 (2001)]. Large-scale
analysis of synthetic lethal interactions provides an opportunity for
functionally classifying genes and should generate a network of genetic
interactions that connects processes and pathways critical for fitness
of the organism. We will present the results of a number of screens
derived from query genes whose products participate in the establishment
of cell polarity and chromosome dynamics. Bioinformatics tools, such as
the Pajek program for Network display and hierarchical clustering allow
visualization of the topology of these interactions, functional
classification of genes of uncharacterized function, and comparison to
orthogonal data sets, such as the yeast protein-protein interaction data
set.
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