Analysis of gene function using Phenotype Microarrays.
Richard Kostriken
Research and Development, Biolog Inc., 3938 Trust Way, Hayward, CA
94545, USA
The Biolog Phenotype Microarray (PM) technology has been employed to
measure the effect of gene disruptions on the metabolism of the yeast
Saccharomyces cerevisiae under thousands of growth conditions. We have
focused our attention primarily on two classes of genes. The first class
is composed of relatively well studied genes-- these serve to establish a
phenotypic baseline. The second class is composed of all the non
essential genes from chromosome I-- these represent a random selection of
non essential genes. Comparison of the PM data obtained from these two
classes of genes provides us with a better understand the relationship
between genotype and phenotype and has allowed us to discover subtle,
previously unrecognized phenotypes in genes defined solely by their open
reading frames.
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