DENDRICHIPS: a New Chip Design based on a novel chemical
functionnalisation, which allows higher sensitivity and reusability of
DNA-arrays.
Veronique Leberre-Anton (1), Emmanuelle Trevisiol (2), Adilia
Dagkessamanskaia (1), Bernard Meunier (3), Jean François (1)
(1) CBGD & Genomic Plateform, INSA/University, 135 Av Rangeuil,
Toulouse, 31077, FRANCE;
(2) Laboratoire de Chimie de Coordination, 205 Rte de
Narbonne,Toulouse.;
(3) Laboratoire de Chimie de Coordination, 205 Rte de Narbonne,
Toulouse
One of our Research project at the Genomic Platform in Toulouse is
concerning the design of better grafting of the DNA on the glass slides
to increase both the ratio signal/noise, to obtain higher
reproducibility and to be able to reuse the arrays (at least for
research purposes). To this end, we developed a new chemical method (the
dendrimer technology), whose a EP patent is being obtained Basically,
the principle is to bind successively, and in a covalent way, first the
dendrimer on a functionalised slide (dendrislide), then the nucleic
acids (PCR or oligonucleotides) on the dendrislide to make the
dendrichip. As compared to other homemade or commercially available
arrays, the main advantages of our technology are as follows. (1)
Increase of the sensitivity (signal ratio/noise) by more than 10-fold.
(2) Reduction by 10-50-fold of the amount of targets (PCR and oligos)
spotted on the dendrislides to reach a equivalent signal as with other
DNA microarrays. (3) Ability of re-using at least 10-fold the same
dendrichips without decreasing quality and sensitivity of hybridisation.
This gives the possibility to repeat the experiment several times in
order to obtain statistically reliable results without significant
increase of the cost. We will provide comparative results with
experiments carried on with yeastgenome.
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