KRE5/YOR336W Single Page Format

This page provides an alternative format to the SGD Locus Summary Page. Note that additional information may be available on or linked from the standard format SGD Locus Summary page.

Contents
Names and Identifiers

GO Annotations

Pathways

Summary Paragraph

Mutant Phenotypes

Interactions

Homologs

Protein Info (physical properties, transcript info)

PDB Homologs (protein structure info)

Motifs

Genome-wide Expression
(and other large-scale analyses)

Locus History (misc. notes)

Sequence Retrieval and Analysis

Map and Displays

Localization

Community Annotation

Literature Guide

Sequence Coordinates

ChrXV: 949770 to 953867
CDS: 949770 - 953867

Click on map for expanded view

SGD ORF map GBrowse
SGD Locus Page

Names and Identifiers [TOP] [NEXT]
Standard Name Systematic Name Alias Feature Type SGDID

KRE5 YOR336W ORF, Verified S000005863

Description
Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects

GO Annotations [TOP] [NEXT]
Molecular Function
Annotation(s) Reference(s) Evidence Assigned By
UDP-glucose:glycoprotein glucosyltransferase activity Shahinian S, et al. (1998) Involvement of protein N-glycosyl chain glucosylation and processing in the biosynthesis of cell wall beta-1,6-glucan of Saccharomyces cerevisiae. Genetics 149(2):843-56
       IDA : Inferred from Direct Assay
Assigned on 2002-09-27 SGD
DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR009448
Assigned on 2007-05-23 UniProtKB
Biological Process
Annotation(s) Reference(s) Evidence Assigned By
1,6-beta-glucan biosynthetic process Yabe T, et al. (1996) HKR1 encodes a cell surface protein that regulates both cell wall beta-glucan synthesis and budding pattern in the yeast Saccharomyces cerevisiae. J Bacteriol 178(2):477-83
       TAS : Traceable Author Statement
Assigned on 2002-09-27 SGD
cellular cell wall organization GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0961
Assigned on 2008-02-14 UniProtKB
protein amino acid glycosylation DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR009448
Assigned on 2007-05-23 UniProtKB
Cellular Component
Annotation(s) Reference(s) Evidence Assigned By
endoplasmic reticulum Montijn RC, et al. (1999) Localization of synthesis of beta1,6-glucan in Saccharomyces cerevisiae. J Bacteriol 181(24):7414-20
       TAS : Traceable Author Statement
Assigned on 2002-09-27 SGD
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0256
Assigned on 2009-10-01 UniProtKB
endoplasmic reticulum lumen GOA curators and UniProt curators (2007) Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping.
     IEA : Inferred from Electronic Annotation with EBI:SL-0096
Assigned on 2008-02-13 UniProtKB

Pathways [TOP] [NEXT]
No pathways available

Summary Paragraph [TOP] [NEXT]
No summary paragraph available

Basic References [TOP]
BASIC INFORMATION REFERENCES forKRE5/YOR336W for KRE5
1) Meaden P, et al. (1990) The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1----6)-beta-D-glucan synthesis and normal cell growth. Mol Cell Biol 10(6):3013-9

2) Al-Aidroos K and Bussey H (1978) Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor. Can J Microbiol 24(3):228-37

Mutant Phenotypes [TOP] [NEXT]
Phenotype page for KRE5/YOR336W

Interactions: genetic, physical, and other gene-gene links. [TOP] [NEXT]
Interaction page for KRE5/YOR336W

Homologs [TOP] [NEXT]

Comparison Resources

Physical Properties and Transcript Information: predicted from sequence [TOP] [NEXT]
Protein Sequence Calculations
from Predicted Full length Translation

N-term MRLLALV

C-term APLHDEL

Length(aa) 1,365

MW(Da) 156,476

pI 4.88

Amino Acid Composition (full length)

GCG tools: PepPlot, Helical Wheel, PepStruct

Transcript Translation Calculations

Codon Bias 0.042

Codon Adaptation Index 0.146

Frequency of Optimal Codons 0.437

Hydropathicity of Protein -0.266

Aromaticity Score 0.099

10 20 30 40 50 | | | | | MRLLALVLLLLCAPLRAWTYSLRYGIPESAQVWSILVHLLGDVDNQLLTN LYPLVTGLDDEIDIQENLVALTSNVLRERYDKEDVADLLELYASLYPMGM IQHDISSNAEQDDANSSYFVLNGNRYEKPDDVFYLKSKDLTIQQKVPDVD VIQPYDVVIGTNSEAPILILYGCPTVIDSDFEEFNRNLFMEAMNGEGKFR FIWRSTCSLDGKSVEYPLTHPLEITLQNGSRMSSIPQLKKILYTVPKEIL VGADNDDQLHDLEPEELRELDLRVTSLISEFYQYKKDITATLNFTKSIVN NFPLISKQLIKVSSVNKDIITSNEELNSKGFDYNMLGLYINGQNWKITSL TPYNLLTALKTEYQSLLKITNLLQELEPSKCILDSKFLLNKFSQFSLGKL QNLQPIKMDLHTIPGFSESVIYFNDIESDPQYDELVNSVQAFFDKSKFGE LPEIKQNWSEIIFVIDFARLEDSEVKEALGGLVRAVNVVSQGYPQRVGLL PFSSDSDKSVVNKIYELKNSTDNLTELKSFLETMLLADGLSANAKHSKHI PVPDVFHLLDELQIDETSIIINGEIYPFRKNAWNYLIAKVIKKDTEFIRK ELSNSSPKNKQISVRDLLHYKSANLRHNKYTPNYFADSVYSSVNNTALES VCSERIGYYTKNEEYNLLHTITLVDDFGSIHALKRLRNLLHTSFVGVRIR IIHVGDISDIWYQLRGSLSQKDPIGSINTFIDALKLKKVKSHTYKKSGLN QLGLHKWLPDIPLFELQKGSFIALNGRFIHLDQNEVPETEHFEAIIKREA LRTIDSVFALDLLFPGFSQEIINPDLIEMISSILTRLFYQGTHIYNNGID YTTESSLPRMDLSEFFRPNNLTMFEDGKSASIDLLLILDPLEERTQMILS LVEQFRPLKFVNIQVILMPTLELNIVPIRRIYVDDADIVKSITSEDSRSD PEVDIEMDVPNSFIVDNNYRIKKLLIELHSFSSKTVLSTGNIDGMGGVCL ALVDSAGNIIDKTTTMKTFGYGQFHTDKFLKGCYIKSCDSRYTVQSFSTD GHPDFIPSDSLDILSYNPQKIAVKISEEPTHEEEYEEGRNNDTIINIFTI LESGPDEEERYMQMILSILSKCPETQKVNFFILDQPFISDTLRKSCEYIN SSDEMRGNVIFLNYEWPQWLRPQRFSSRRRDVSRFLFLDVLLPQNISKVL YMSPTEVPLDPFDIFQFQGLKRAPLGLFRMSGDGYWKEGYWEKMLRENNL EFYSTEPAFLVNLERFRELDAGDKYRIHYQRISTDAMSLVNIGQDLVNNL QLEVPIRFLKGSYKKKLVINDECVSEWKKKINKFASSPGDEDVPGESVSS KYQDSDNAAPLHDEL*

Protein Structures from PDB: proteins of known structure with sequence similarity to KRE5/YOR336W, based on Smith-Waterman analysis. [TOP] [NEXT]
No protein structure information available.

Genome-wide Expression and Other Large-Scale Analyses [TOP] [NEXT]

Functional Analysis
You can also search multiple datasets simultaneously using Expression Connection for expression studies or Function Junction for other large scale analyses.

Locus History (misc. notes) [TOP] [NEXT]
Nomenclature History
Standard Name Reference
KRE5 Meaden P, et al. (1990) The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1----6)-beta-D-glucan synthesis and normal cell growth. Mol Cell Biol 10(6):3013-9

Standard Name	Reference
KRE5	*Meaden P, et al.* (1990)** The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1----6)-beta-D-glucan synthesis and normal cell growth. Mol Cell Biol 10(6):3013-9

Sequence Retrieval [TOP] [NEXT]
Sequence Type Output Format

Genomic DNA GCG | FASTA | NoHeader

Genomic DNA with 1 kb up and downstream GCG | FASTA | NoHeader

DNA coding sequence
(without introns, without flanking regions) GCG | FASTA | NoHeader

Protein Translation of ORF GCG | FASTA | NoHeader

6-Frame Translation(with Restriction Map) GCG

Restriction Fragment Sizes GCG

Sequence Analysis Tools
Sequence from other databases

Sequence ID Source

YOR336W SGD Systematic Sequence

854514 NCBI: Gene ID

NP_014981.1 NCBI: RefSeq protein version ID

NP_014981.1 NCBI: RefSeq protein version ID

6324912 NCBI: NCBI protein GI

Sequence from other databases
Sequence ID	Source
YOR336W	SGD Systematic Sequence
854514	NCBI: Gene ID
NP_014981.1	NCBI: RefSeq protein version ID
NP_014981.1	NCBI: RefSeq protein version ID
6324912	NCBI: NCBI protein GI

Map and Displays [TOP] [NEXT]
Physical, Genetic Maps: Chromosomal Feature Map GBrowse Combined Physical and Genetic Map Genetic Distance vs. Physical Distance Ratios
Similarity Viewers: Synteny Viewer Genomic Stripe View SAGE Results Map

Localization [TOP] [NEXT]

Localization Resources

Community Annotation [TOP] [NEXT]
No community annotation available.

Literature Guide: papers categorized by topic. [TOP]
Topics Reference Other Genes Addressed
24 curated references; 0 references not yet curated
Mutants/Phenotypes
Strains/Constructs
Aimanianda V, et al. (2009) Cell Wall {beta}-(1,6)-Glucan of Saccharomyces cerevisiae: STRUCTURAL CHARACTERIZATION AND IN SITU SYNTHESIS. J Biol Chem 284(20):13401-12
       |KRE6 |KRE9
Reviews
Joshi AS, et al. (2009) Cellular functions of cardiolipin in yeast. Biochim Biophys Acta 1793(1):212-8
       |COX4 |CRD1 |ISC1 |PGS1 |SLT2 |TAZ1
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Zhou J, et al. (2009) Loss of cardiolipin leads to longevity defects that are alleviated by alterations in stress response signaling. J Biol Chem 284(27):18106-14
       |CRD1 |HSP12 |MSN2 |PBS2 |PGS1 |SHO1 |SSK2 |SSK22 |STE50 |YAP1
Mutants/Phenotypes
Strains/Constructs
Breslow DK, et al. (2008) A comprehensive strategy enabling high-resolution functional analysis of the yeast genome. Nat Methods 5(8):711-8
       |AAR2 |ABD1 |ABF1 |ACC1 |ACP1 |ADE13 |AFG2 |ALA1 |ALG1 |ALG13 |ALG14 |ALG2 |ALG7 |ALR1 |MORE
Genomic expression study
RNA Levels and Processing
Del Vescovo V, et al. (2008) Role of Hog1 and Yaf9 in the transcriptional response of Saccharomyces cerevisiae to cesium chloride. Physiol Genomics 33(1):110-20
       |AGA1 |ALD3 |ARO10 |ATG1 |BRL1 |CAR2 |CMK2 |COX5B |CPR1 |CPR7 |CWP1 |CYC7 |DAN4 |DDR48 |MORE
Cellular Location
Protein Processing/Modification/Regulation
Protein-protein Interactions
Regulation of
Strains/Constructs
Takeuchi M, et al. (2008) Saccharomyces cerevisiae Rot1 Is an Essential Molecular Chaperone in the Endoplasmic Reticulum. Mol Biol Cell 19(8):3514-25
       |DRS2 |GUP1 |KAR2 |KRE6 |ROT1 |UBC7
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Zhong Q, et al. (2007) Up-regulation of the cell integrity pathway in saccharomyces cerevisiae suppresses temperature sensitivity of the pgs1Delta mutant. J Biol Chem 282(22):15946-53
         |BCK1 |FKS1 |GSC2 |MKK1 |PGS1 |PKC1 |RHO1 |ROM2 |SLT2
Reviews
Lesage G and Bussey H (2006) Cell wall assembly in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 70(2):317-43
       |ANP1 |BGL2 |BIG1 |CDA1 |CDA2 |CHS1 |CHS2 |CHS3 |CHS6 |CHS7 |CNE1 |CRH1 |CRR1 |CTS1 |MORE
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Zhong Q, et al. (2005) Loss of function of KRE5 suppresses temperature sensitivity of mutants lacking mitochondrial anionic lipids. Mol Biol Cell 16(2):665-75
         |PGS1
Cross-species Expression
Function/Process
Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Herrero AB, et al. (2004) KRE5 gene null mutant strains of Candida albicans are avirulent and have altered cell wall composition and hypha formation properties. Eukaryot Cell 3(6):1423-32

Mutants/Phenotypes
Machi K, et al. (2004) Rot1p of Saccharomyces cerevisiae is a putative membrane protein required for normal levels of the cell wall 1,6-beta-glucan. Microbiology 150(Pt 10):3163-73
       |BIG1 |FLO1 |ROT1
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Chavan M, et al. (2003) Genetic, biochemical, and morphological evidence for the involvement of N-glycosylation in biosynthesis of the cell wall beta1,6-glucan of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 100(26):15381-6
       |KRE9 |STT3
Function/Process
Genetic Interactions
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Azuma M, et al. (2002) Saccharomyces cerevisiae Big1p, a putative endoplasmic reticulum membrane protein required for normal levels of cell wall beta-1,6-glucan. Yeast 19(9):783-93
       |BIG1
Cellular Location
Function/Process
Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Levinson JN, et al. (2002) Functional, comparative and cell biological analysis of Saccharomyces cerevisiae Kre5p. Yeast 19(14):1243-59
       |KRE6
DNA/RNA Sequence Features
Davis CA, et al. (2000) Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast. Nucleic Acids Res 28(8):1700-6
       |AIM11 |AMA1 |AML1 |APE2 |APS3 |ARP9 |ASC1 |BET1 |BIG1 |BOS1 |CDC21 |COF1 |DCN1 |DID4 |MORE
RNA Levels and Processing
Braley R and Chaffin WL (1999) Variations in mRNA transcript levels of cell wall-associated genes of Saccharomyces cerevisiae following spheroplasting. FEMS Microbiol Lett 181(1):177-85
       |CRH1 |EGT2 |EXO1 |FLO1 |HKR1 |KRE1 |KRE11 |MSN1 |MUC1 |PMT2 |SEC53 |SED1 |SKT5 |TIR1
Fungal Related Genes/Proteins
Mutants/Phenotypes
Other Features
Strains/Constructs
Castro O, et al. (1999) Uridine diphosphate-glucose transport into the endoplasmic reticulum of Saccharomyces cerevisiae: in vivo and in vitro evidence. Mol Biol Cell 10(4):1019-30
       |ALG5 |ALG6
Cellular Location
Function/Process
Montijn RC, et al. (1999) Localization of synthesis of beta1,6-glucan in Saccharomyces cerevisiae. J Bacteriol 181(24):7414-20
       |KRE6 |SEC1 |SKN1
Function/Process
Shahinian S, et al. (1998) Involvement of protein N-glycosyl chain glucosylation and processing in the biosynthesis of cell wall beta-1,6-glucan of Saccharomyces cerevisiae. Genetics 149(2):843-56
       |CNE1 |CWH41
DNA/RNA Sequence Features
Parle-McDermott AG, et al. (1996) Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces cerevisiae chromosome XV: similarity to part of chromosome I. Yeast 12(10B Suppl):999-1004
     |ALA1 |FRT1 |FRT2 |FUN19 |MIP1 |MRS2 |MYO2 |PDR10 |PDR5 |SCD5 |SNC2 |TEA1 |VMA4 |YOR338W |MORE
DNA/RNA Sequence Features
Function/Process
Fungal Related Genes/Proteins
Yabe T, et al. (1996) HKR1 encodes a cell surface protein that regulates both cell wall beta-glucan synthesis and budding pattern in the yeast Saccharomyces cerevisiae. J Bacteriol 178(2):477-83
       |HKR1
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Brown JL and Bussey H (1993) The yeast KRE9 gene encodes an O glycoprotein involved in cell surface beta-glucan assembly. Mol Cell Biol 13(10):6346-56
     |KRE1 |KRE11 |KRE6 |KRE9 |SKN1
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Boone C, et al. (1990) Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly. J Cell Biol 110(5):1833-43
     |KRE1 |KRE6
Cellular Location
Function/Process
Genetic Interactions
Mutants/Phenotypes
Protein Physical Properties
Protein Sequence Features
Strains/Constructs
Meaden P, et al. (1990) The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1----6)-beta-D-glucan synthesis and normal cell growth. Mol Cell Biol 10(6):3013-9
     |KRE6

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