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PRM6/YML047C Single Page Format

This page provides an alternative format to the SGD Locus Summary Page. Note that additional information may be available on or linked from the standard format SGD Locus Summary page.

Contents

Names and Identifiers GO Annotations Pathways Summary Paragraph Mutant Phenotypes Interactions Homologs Protein Info (physical properties, transcript info) PDB Homologs (protein structure info) Motifs Genome-wide Expression (and other large-scale analyses) Locus History (misc. notes) Sequence Retrieval and Analysis Map and Displays Localization Community Annotation Literature Guide

Sequence Coordinates   ChrXIII: 181075 to 180017 CDS: 181075 - 180017 Click on map for expanded view SGD ORF map GBrowse

SGD Locus Page

Names and Identifiers [TOP] [NEXT]
Standard Name	Systematic Name	Alias	Feature Type	SGDID
PRM6	YML047C		ORF, Verified	S000004510
This name is reserved with SGD according to the Gene Naming Guidelines agreed upon by the yeast community. Gene name reserved on: 2000-09-05. Gene name expires on 2001-09-05.
Description
Pheromone-regulated protein, predicted to have 2 transmembrane segments; regulated by Ste12p during mating

GO Annotations [TOP] [NEXT]
Molecular Function Annotation(s) Reference(s) Evidence Assigned By molecular_function unknown SGD (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD    ND : No Biological Data Available Assigned on 2001-01-19 SGD
Biological Process Annotation(s) Reference(s) Evidence Assigned By biological_process unknown SGD (2002) Use of the ND evidence code for Gene Ontology (GO) terms in SGD    ND : No Biological Data Available Assigned on 2009-08-25 SGD sexual reproduction Huttenhower C and Troyanskaya OG (2009) Prediction of Gene Ontology annotations by integrating high-throughput datasets    RCA : Reviewed Computational Analysis Assigned on 2009-08-06 bioPIXIE_MEFIT
Cellular Component Annotation(s) Reference(s) Evidence Assigned By integral to membrane Heiman MG and Walter P (2000) Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating. J Cell Biol 151(3):719-30        ISS : Inferred from Sequence or structural Similarity Assigned on 2001-01-18 SGD GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.      IEA : Inferred from Electronic Annotation with EBI:KW-0812 Assigned on 2007-05-23 UniProtKB GOA curators and UniProt curators (2007) Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping.      IEA : Inferred from Electronic Annotation with EBI:SL-9909 Assigned on 2009-10-01 UniProtKB membrane GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.      IEA : Inferred from Electronic Annotation with EBI:KW-0472 Assigned on 2009-06-29 UniProtKB

Pathways [TOP] [NEXT]
No pathways available

Summary Paragraph [TOP] [NEXT]
No summary paragraph available

Basic References [TOP]
BASIC INFORMATION REFERENCES forPRM6/YML047C for PRM6 1) Heiman MG and Walter P (2000) Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating. J Cell Biol 151(3):719-30        2) White JM and Rose MD (2001) Yeast mating: getting close to membrane merger. Curr Biol 11(1):R16-20

Mutant Phenotypes [TOP] [NEXT]
Phenotype page for PRM6/YML047C

Interactions: genetic, physical, and other gene-gene links. [TOP] [NEXT]
Interaction page for PRM6/YML047C

Homologs [TOP] [NEXT]
Comparison Resources PSI-BLAST Results Ashbya Homologs (AGD) BLASTN vs. fungi BLASTP at NCBI BLASTP vs. fungi Fungal Alignment Fungal Orthogroups Repository Ortholog Search (P-POD) PhylomeDB Synteny Viewer Yeast Gene Order Browser (YGOB) eukarYotic OrtholoGY (YOGY)

Physical Properties and Transcript Information: predicted from sequence [TOP] [NEXT]
Protein Sequence Calculations from Predicted Full length Translation N-term MESSLQK C-term KYKHKYI Length(aa) 352 MW(Da) 41,372 pI 8.77 Amino Acid Composition (full length) GCG tools: PepPlot, Helical Wheel, PepStruct Transcript Translation Calculations Codon Bias -0.044   Codon Adaptation Index 0.113   Frequency of Optimal Codons 0.409   Hydropathicity of Protein 0.076   Aromaticity Score 0.148	10 20 30 40 50 | | | | | MESSLQKLKFQDIDINLIPTAKWTTKLQYILYTWCQSILHVAMFFSDIYT CIKLLAFNTWSNNIIQPFLEFRISKWLFSGCILCSSLILIWELVIGLRVY RKKEITSNYMNGISRLINCLFNFKKYQIFELIVLTDEKKFSKWLFFSYFE ISGCLRLLFGDSPRQIINGLTLWSVLLTVSNETSSGTHSTQSLGNLDDLN GIINKIKHIAKTNYEESVILSFMLFSFIIWVILISKLILSIIIFIIFIRP RFLSSKRKVKGYELKLRKYVSKVIDENLSRTVYELGILIDDEEEGTICGD NKTQKKFDYDSPDYGDESTIPSYYCYSDVETYERVYTPIKAYFPQKYKHK YI*

Protein Structures from PDB: proteins of known structure with sequence similarity to PRM6/YML047C, based on Smith-Waterman analysis. [TOP] [NEXT]
No protein structure information available.

Genome-wide Expression and Other Large-Scale Analyses [TOP] [NEXT]
Functional Analysis Expression Connection Summary Cell cycle and metabolic oscillation Cell cycle transcript profile Cyclebase Genevestigator GermOnline SPELL Microarray Search YGAC Triples Yeast Microarray Global Viewer YeastFunc	You can also search multiple datasets simultaneously using Expression Connection for expression studies or Function Junction for other large scale analyses.

Locus History (misc. notes) [TOP] [NEXT]

Nomenclature History

Standard Name	Date Standardized	Reference
PRM6	2000-10-30	Heiman MG and Walter P (2000) Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating. J Cell Biol 151(3):719-30

Sequence Retrieval [TOP] [NEXT]
Sequence Type	Output Format
Genomic DNA	GCG | FASTA | NoHeader
Genomic DNA with 1 kb up and downstream	GCG | FASTA | NoHeader
DNA coding sequence (without introns, without flanking regions)	GCG | FASTA | NoHeader
Protein Translation of ORF	GCG | FASTA | NoHeader
6-Frame Translation(with Restriction Map)	GCG
Restriction Fragment Sizes	GCG
Sequence Analysis Tools BLASTP BLASTN Design Primers FASTA aa FASTA nt Restriction Fragment Map Restriction Fragment Sizes Six-Frame Translation

Sequence from other databases
Sequence ID	Source
YML047C	SGD Systematic Sequence
854959	NCBI: Gene ID
NP_013666.1	NCBI: RefSeq protein version ID
NP_013666.1	NCBI: RefSeq protein version ID
6323595	NCBI: NCBI protein GI

Map and Displays [TOP] [NEXT]
Physical, Genetic Maps:	Chromosomal Feature Map	GBrowse	Combined Physical and Genetic Map	Genetic Distance vs. Physical Distance Ratios
Similarity Viewers:	Synteny Viewer	Genomic Stripe View	SAGE Results Map

Localization [TOP] [NEXT]
Localization Resources YGAC Triples YeastGFP DB (UCSF) at SGD

Community Annotation [TOP] [NEXT]
No community annotation available.

Literature Guide: papers categorized by topic. [TOP]
Topics	Reference	Other Genes Addressed
10 curated references; 0 references not yet curated
RNA Levels and Processing Regulation of Transcription	Kitchen CM, et al. (2009) The mating response cascade does not modulate changes in the steady-state level of target mRNAs through control of mRNA stability. Yeast 26(5):261-72	|BAR1 |ECM18 |FIG1 |FIG2 |FUS1 |FUS2 |PRM2 |RPO21 |STE12
Regulation of Transcription	Rintala E, et al. (2009) Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiae. BMC Genomics 10():461	|AAT2 |ACO1 |ACS1 |ADH1 |ADH2 |AFR1 |AGA1 |AGA2 |ALD4 |ALD6 |ANT1 |ARE1 |ARN1 |ASH1 |MORE
Genetic Interactions Mutants/Phenotypes Strains/Constructs	Hausmann S, et al. (2008) Genetic and Biochemical Analysis of Yeast and Human Cap Trimethylguanosine Synthase: FUNCTIONAL OVERLAP OF 2,2,7-TRIMETHYLGUANOSINE CAPS, SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENTS, PRE-mRNA SPLICING FACTORS, AND RNA DECAY PATHWAYS. J Biol Chem 283(46):31706-18	|BRR1 |BUD13 |CWC21 |HTZ1 |IST3 |ISY1 |LEA1 |LSM1 |MRM2 |MUD1 |MUD2 |NAM8 |PAP2 |PAT1 |MORE
Mutants/Phenotypes Strains/Constructs Techniques and Reagents	Butcher RA, et al. (2006) Microarray-based method for monitoring yeast overexpression strains reveals small-molecule targets in TOR pathway. Nat Chem Biol 2(2):103-9	|BMH1 |BRE5 |BUD27 |BUD8 |CAJ1 |DCR2 |DGK1 |ELP2 |FET4 |FPR1 |FRE5 |FSP2 |GEP3 |GIC2 |MORE
DNA/RNA Sequence Features Regulation of	Chou S, et al. (2006) Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae. Mol Cell Biol 26(13):4794-805	|AFR1 |AGA1 |ASG7 |BAR1 |CHS7 |CIK1 |CLN1 |CWP1 |DDR48 |DIG1 |DIG2 |FIG1 |FUS1 |FUS2 |MORE
Genetic Interactions Strains/Constructs	Tong AH, et al. (2004) Global mapping of the yeast genetic interaction network. Science 303(5659):808-13	|AAD4 |AAH1 |ABF2 |ACE2 |ADH6 |AEP2 |AFG1 |AGP1 |AHC1 |AHC2 |AIM21 |AIM22 |AIM26 |AIM29 |MORE
Regulation of	Zeitlinger J, et al. (2003) Program-specific distribution of a transcription factor dependent on partner transcription factor and MAPK signaling. Cell 113(3):395-404	|ACT1 |ADE1 |AFR1 |AGA1 |ASG7 |BEM1 |BEM2 |BMH1 |BUD14 |BUD8 |CHS1 |CHS7 |CIK1 |CLA4 |MORE
RNA Levels and Processing Regulation of	Harris K, et al. (2001) Role of scaffolds in MAP kinase pathway specificity revealed by custom design of pathway-dedicated signaling proteins. Curr Biol 11(23):1815-24	|AFR1 |AGA1 |ARI1 |ASG7 |CIK1 |CRG1 |CWP1 |DAK1 |DSF1 |FUS1 |FUS2 |GPD1 |GRE2 |HYM1 |MORE
Reviews	White JM and Rose MD (2001) Yeast mating: getting close to membrane merger. Curr Biol 11(1):R16-20	|PRM1 |PRM2 |PRM3 |PRM4 |VAM3 |VAM7
Function/Process Protein Sequence Features Regulation of	Heiman MG and Walter P (2000) Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating. J Cell Biol 151(3):719-30	|AGA1 |PRM1 |PRM10 |PRM2 |PRM3 |PRM4 |PRM5 |PRM7 |PRM8 |PRM9

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