ERG6/YML008C Single Page Format

This page provides an alternative format to the SGD Locus Summary Page. Note that additional information may be available on or linked from the standard format SGD Locus Summary page.

Contents
Names and Identifiers

GO Annotations

Pathways

Summary Paragraph

Mutant Phenotypes

Interactions

Homologs

Protein Info (physical properties, transcript info)

PDB Homologs (protein structure info)

Motifs

Genome-wide Expression
(and other large-scale analyses)

Locus History (misc. notes)

Sequence Retrieval and Analysis

Map and Displays

Localization

Community Annotation

Literature Guide

Sequence Coordinates

ChrXIII: 252990 to 251839
CDS: 252990 - 251839
Genetic position: 2 cM
Genetic Map Data

Click on map for expanded view

SGD ORF map GBrowse
SGD Locus Page

Names and Identifiers [TOP] [NEXT]
Standard Name Systematic Name Alias Feature Type SGDID

ERG6 YML008C ISE1, LIS1, SED6, VID1 ORF, Verified S000004467

Description
Delta(24)-sterol C-methyltransferase, converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to both lipid particles and mitochondrial outer membrane

GO Annotations [TOP] [NEXT]
Molecular Function
Annotation(s) Reference(s) Evidence Assigned By
methyltransferase activity DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR013216 , EBI:IPR013705
Assigned on 2007-05-23 UniProtKB
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0489
Assigned on 2007-05-23 UniProtKB
sterol 24-C-methyltransferase activity GOA curators and MGI curators (2001) Gene Ontology annotation based on Enzyme Commission mapping.
     IEA : Inferred from Electronic Annotation with IUBMB:2.1.1.41
Assigned on 2007-05-23 UniProtKB
Jayasimha P and Nes WD (2008) Photoaffinity Labeling and Mutational Analysis of 24-C-Sterol Methyltransferase Defines the AdoMet Binding Site. Lipids 43(8):681-93
     IDA : Inferred from Direct Assay
Assigned on 2008-07-18 SGD
transferase activity GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0808
Assigned on 2007-05-23 UniProtKB
Biological Process
Annotation(s) Reference(s) Evidence Assigned By
alcohol metabolic process Huttenhower C and Troyanskaya OG (2009) Prediction of Gene Ontology annotations by integrating high-throughput datasets
   RCA : Reviewed Computational Analysis
Assigned on 2009-08-06 bioPIXIE_MEFIT
cellular metabolic compound salvage Huttenhower C and Troyanskaya OG (2009) Prediction of Gene Ontology annotations by integrating high-throughput datasets
   RCA : Reviewed Computational Analysis
Assigned on 2009-08-06 bioPIXIE_MEFIT
ergosterol biosynthetic process Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
   TAS : Traceable Author Statement
Assigned on 2001-01-19 SGD
lipid biosynthetic process GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0444
Assigned on 2007-05-23 UniProtKB
metabolic process DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR013216
Assigned on 2007-05-23 UniProtKB
steroid biosynthetic process DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR013705
Assigned on 2009-01-12 UniProtKB
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0752
Assigned on 2007-05-23 UniProtKB
sterol biosynthetic process GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0756
Assigned on 2007-05-23 UniProtKB
Cellular Component
Annotation(s) Reference(s) Evidence Assigned By
endoplasmic reticulum Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
   TAS : Traceable Author Statement
Assigned on 2001-01-19 SGD
lipid particle Athenstaedt K, et al. (1999) Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae. J Bacteriol 181(20):6441-8
       IDA : Inferred from Direct Assay
Assigned on 2001-01-18 SGD
mitochondrial outer membrane Zahedi RP, et al. (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50
         IDA : Inferred from Direct Assay
Assigned on 2006-03-17 SGD
mitochondrion Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
         IDA : Inferred from Direct Assay
Assigned on 2004-09-28 SGD
Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
           IDA : Inferred from Direct Assay
Assigned on 2006-12-12 SGD

Pathways [TOP] [NEXT]
ergosterol biosynthesis

Summary Paragraph [TOP] [NEXT]
SUMMARY PARAGRAPH for ERG6/YML008C for ERG6
ERG6 encodes delta(24)-sterol C-methyltransferase, which converts zymosterol to fecosterol by methylating C-24 in the ergosterol biosynthetic pathway (1, 2, 3, 4). Cells lacking ERG6 are viable but unable to methylate sterols on C-24, and exhibit pleiotropic growth phenotypes (1). erg6 mutants show increased cation uptake (5), and are permeable and therefore sensitive to brefeldin A, a drug that disrupts secretion (6, 7). Enzymes similar to Erg6p have been identified in other organisms; a homolog from Candida albicans complements the S. cerevisiae erg6 null phenotype, and a homologous enzyme from Arabidopsis is active in yeast (8, 9).
Last Updated: 2000-09-05

Basic References [TOP]
BASIC INFORMATION REFERENCES forERG6/YML008C for ERG6
1) Gaber RF, et al. (1989) The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol Cell Biol 9(8):3447-56

2) Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press

3) Lees ND, et al. (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6

4) Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30

5) Welihinda AA, et al. (1994) Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae. Biochim Biophys Acta 1193(1):107-17

6) Graham TR, et al. (1993) Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway. EMBO J 12(3):869-77

7) Shah N and Klausner RD (1993) Brefeldin A reversibly inhibits secretion in Saccharomyces cerevisiae. J Biol Chem 268(8):5345-8

8) Jensen-Pergakes KL, et al. (1998) Sequencing, disruption, and characterization of the Candida albicans sterol methyltransferase (ERG6) gene: drug susceptibility studies in erg6 mutants. Antimicrob Agents Chemother 42(5):1160-7

9) Husselstein T, et al. (1996) Transformation of Saccharomyces cerevisiae with a cDNA encoding a sterol C-methyltransferase from Arabidopsis thaliana results in the synthesis of 24-ethyl sterols. FEBS Lett 381(1-2):87-92

10) Zahedi RP, et al. (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50

Mutant Phenotypes [TOP] [NEXT]
Phenotype page for ERG6/YML008C

Interactions: genetic, physical, and other gene-gene links. [TOP] [NEXT]
Interaction page for ERG6/YML008C

Homologs [TOP] [NEXT]

Comparison Resources

Physical Properties and Transcript Information: predicted from sequence [TOP] [NEXT]
Protein Sequence Calculations
from Predicted Full length Translation

N-term MSETELR

C-term TSQEATQ

Length(aa) 383

MW(Da) 43,431

pI 5.6

Amino Acid Composition (full length)

GCG tools: PepPlot, Helical Wheel, PepStruct

Transcript Translation Calculations

Codon Bias 0.340

Codon Adaptation Index 0.308

Frequency of Optimal Codons 0.608

Hydropathicity of Protein -0.550

Aromaticity Score 0.123

10 20 30 40 50 | | | | | MSETELRKRQAQFTRELHGDDIGKKTGLSALMSKNNSAQKEAVQKYLRNW DGRTDKDAEERRLEDYNEATHSYYNVVTDFYEYGWGSSFHFSRFYKGESF AASIARHEHYLAYKAGIQRGDLVLDVGCGVGGPAREIARFTGCNVIGLNN NDYQIAKAKYYAKKYNLSDQMDFVKGDFMKMDFEENTFDKVYAIEATCHA PKLEGVYSEIYKVLKPGGTFAVYEWVMTDKYDENNPEHRKIAYEIELGDG IPKMFHVDVARKALKNCGFEVLVSEDLADNDDEIPWYYPLTGEWKYVQNL ANLATFFRTSYLGRQFTTAMVTVMEKLGLAPEGSKEVTAALENAAVGLVA GGKSKLFTPMMLFVARKPENAETPSQTSQEATQ*

Protein Structures from PDB: proteins of known structure with sequence similarity to ERG6/YML008C, based on Smith-Waterman analysis. [TOP] [NEXT]
PDB protein structure(s) homologous to ERG6 Homolog Source (per PDB) Protein Alignment: ERG6 vs. Homolog External Links
P-Value %Identical %Similar Alignment
3bus ( Chain: B, A)
Crystal structure of rebm
PDB_Info
PDB_Structure
Lechevalieria aerocolonigenes Chain B = 1.8e-12 26 31 View alignment SCOP
MMDB
CATH
Chain A = 1.8e-12 26 31 View alignment
2o57 ( Chain: C, A, B, D)
Crystal structure of a putative sarcosine dimethylglycine methyltransferase from galdieria sulphuraria
PDB_Info
PDB_Structure
Galdieria sulphuraria Chain C = 6.0e-10 27 30 View alignment SCOP
MMDB
CATH
Chain A = 6.0e-10 27 30 View alignment
Chain B = 6.0e-10 27 30 View alignment
Chain D = 6.0e-10 27 30 View alignment
1ve3 ( Chain: A, B)
Crystal structure of ph0226 protein from pyrococcus horikoshii ot3
PDB_Info
PDB_Structure
Pyrococcus horikoshii Chain A = 3.1e-08 38 30 View alignment SCOP
MMDB
CATH
Chain B = 3.1e-08 38 30 View alignment
1wzn ( Chain: C, A, B)
Crystal structure of the sam-dependent methyltransferase from pyrococcus horikoshii ot3
PDB_Info
PDB_Structure
Pyrococcus horikoshii OT3 Chain C = 4.8e-05 35 33 View alignment SCOP
MMDB
CATH
Chain A = 4.8e-05 35 33 View alignment
Chain B = 4.8e-05 35 33 View alignment
2p8j ( Chain: A, B)
Crystal structure of s-adenosylmethionine-dependent methyltransferase (np_349143.1) from clostridium acetobutylicum at 2.00 a resolution
PDB_Info
PDB_Structure
Clostridium acetobutylicum Chain A = 0.000149 29 32 View alignment SCOP
MMDB
CATH
Chain B = 0.000149 29 32 View alignment
3e7p ( Chain: A)
Crystal structure of of putative methyltransferase from bacteroides vulgatus atcc 8482
PDB_Info
PDB_Structure
Bacteroides vulgatus ATCC 8482 0.001100 27 31 View alignment SCOP
MMDB
CATH
3gdh ( Chain: C, A, B)
Trimethylguanosine synt
PDB_Info
PDB_Structure
Unknown Chain C = 0.002099 30 38 View alignment SCOP
MMDB
CATH
Chain A = 0.002099 30 38 View alignment
Chain B = 0.002099 30 38 View alignment
3egi ( Chain: B, A, C, D)
Methyltransferase domain of human trimethylguanosine synthase tgs1 bound to m7gpppa (inactive form)
PDB_Info
PDB_Structure
Homo sapiens Chain B = 0.005097 32 35 View alignment SCOP
MMDB
CATH
Chain A = 0.005097 32 35 View alignment
Chain C = 0.005097 32 35 View alignment
Chain D = 0.005097 32 35 View alignment

Genome-wide Expression and Other Large-Scale Analyses [TOP] [NEXT]

Functional Analysis
You can also search multiple datasets simultaneously using Expression Connection for expression studies or Function Junction for other large scale analyses.

Locus History (misc. notes) [TOP] [NEXT]
Nomenclature History
Standard Name Reference
ERG6 SGD (2007) Information without a citation in SGD

Alias Name(s) Reference
VID1 Shieh HL, et al. (2001) Biochemical analysis of fructose-1,6-bisphosphatase import into vacuole import and degradation vesicles reveals a role for UBC1 in vesicle biogenesis. J Biol Chem 276(13):10398-406

Mapping Notes
Date Note
1989-10-01 Edition 10: erg6 was mapped near trp1 on the 1985 map, and this assignment has been shown to be incorrect. It has recently been remapped on chromosome XIIIR
Mortimer RK, et al. (1989) Genetic map of Saccharomyces cerevisiae, edition 10. Yeast 5(5):321-403

Standard Name	Reference
ERG6	SGD (2007) Information without a citation in SGD

Alias Name(s)	Reference
VID1	*Shieh HL, et al.* (2001)** Biochemical analysis of fructose-1,6-bisphosphatase import into vacuole import and degradation vesicles reveals a role for UBC1 in vesicle biogenesis. J Biol Chem 276(13):10398-406

Date	Note
1989-10-01	Edition 10: erg6 was mapped near trp1 on the 1985 map, and this assignment has been shown to be incorrect. It has recently been remapped on chromosome XIIIR *Mortimer RK, et al.* (1989)** Genetic map of Saccharomyces cerevisiae, edition 10. Yeast 5(5):321-403

Sequence Retrieval [TOP] [NEXT]
Sequence Type Output Format

Genomic DNA GCG | FASTA | NoHeader

Genomic DNA with 1 kb up and downstream GCG | FASTA | NoHeader

DNA coding sequence
(without introns, without flanking regions) GCG | FASTA | NoHeader

Protein Translation of ORF GCG | FASTA | NoHeader

6-Frame Translation(with Restriction Map) GCG

Restriction Fragment Sizes GCG

Sequence Analysis Tools
Sequence from other databases

Sequence ID Source

YML008C SGD Systematic Sequence

855003 NCBI: Gene ID

NP_013706.1 NCBI: RefSeq protein version ID

NP_013706.1 NCBI: RefSeq protein version ID

6323635 NCBI: NCBI protein GI

Sequence from other databases
Sequence ID	Source
YML008C	SGD Systematic Sequence
855003	NCBI: Gene ID
NP_013706.1	NCBI: RefSeq protein version ID
NP_013706.1	NCBI: RefSeq protein version ID
6323635	NCBI: NCBI protein GI

Map and Displays [TOP] [NEXT]
Physical, Genetic Maps: Chromosomal Feature Map GBrowse Combined Physical and Genetic Map Genetic Distance vs. Physical Distance Ratios
Similarity Viewers: Synteny Viewer Genomic Stripe View SAGE Results Map

Localization [TOP] [NEXT]

Localization Resources

Community Annotation [TOP] [NEXT]
No community annotation available.

Literature Guide: papers categorized by topic. [TOP]
Topics Reference Other Genes Addressed
171 curated references; 0 references not yet curated
Genetic Interactions
Mutants/Phenotypes
Hodg CA, et al. (2010) Integral membrane proteins Brr6 and Apq12 link assembly of the nuclear pore complex to lipid homeostasis in the endoplasmic reticulum. J Cell Sci 123(Pt 1):141-151
       |ACC1 |APQ12 |ARE1 |ARE2 |ARV1 |BRR6 |DGA1 |ELO1 |ERG2 |ERG3 |ERG4 |ERG5 |FEN1 |LRO1 |MORE
Regulation of
Zeng T and Li J (2010) Maximization of negative correlations in time-course gene expression data for enhancing understanding of molecular pathways. Nucleic Acids Res 38(1):e1
       |ACO2 |ADR1 |CAB2 |CAT8 |CIT1 |ERG2 |ERG26 |ERG27 |ERG4 |ERG5 |GCN4 |GTT1 |HAP1 |HAP3 |MORE
Mutants/Phenotypes
Strains/Constructs
Abe F and Hiraki T (2009) Mechanistic role of ergosterol in membrane rigidity and cycloheximide resistance in Saccharomyces cerevisiae. Biochim Biophys Acta 1788(3):743-52
       |ERG2 |ERG3 |ERG4 |ERG5 |PDR5 |UPC2
Mutants/Phenotypes
Strains/Constructs
Banuelos MG, et al. (2009) Genomic analysis of severe hypersensitivity to hygromycin B reveals linkage to vacuolar defects and new vacuolar gene functions in Saccharomyces cerevisiae. Curr Genet
     |ARF1 |BUD32 |BUR2 |CHC1 |CIN5 |CPA1 |DBP7 |DHH1 |DRS2 |GET2 |GLN3 |HHY1 |ISM1 |MCT1 |MORE
Regulation of
Bruckmann A, et al. (2009) Proteome analysis of aerobically and anaerobically grown Saccharomyces cerevisiae cells. J Proteomics 71(6):662-9
       |ACH1 |ACS1 |ACS2 |ADE17 |ADH1 |ADH2 |ALD4 |ALD6 |APE2 |ARP3 |ASC1 |ASN1 |ATP1 |ATP2 |MORE
Other Features
Connerth M, et al. (2009) Analysis of lipid particles from yeast. Methods Mol Biol 579:359-74
       |AYR1 |ERG1 |ERG7 |POR1 |PRC1 |SEC61 |WBP1
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Daicho K, et al. (2009) Sorting defects of the tryptophan permease Tat2 in an erg2 yeast mutant. FEMS Microbiol Lett 298(2):218-27
       |ERG2 |ERG3 |ERG4 |ERG5 |TAT2 |TRP1
Reviews
Ding J, et al. (2009) Tolerance and stress response to ethanol in the yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol 85(2):253-63
       |ADA2 |ASR1 |ATP1 |BEM2 |CHD1 |CTT1 |DDR2 |ERO1 |FEN1 |GCN5 |GIM4 |GIM5 |HFI1 |HMI1 |MORE
Reviews
Goodman JM (2009) Demonstrated and inferred metabolism associated with cytosolic lipid droplets. J Lipid Res 50(11):2148-56
       |AYR1 |DGA1 |EHT1 |ERG1 |ERG26 |ERG27 |ERG7 |FAA1 |FAA4 |FAT1 |SLC1 |TGL1 |TGL3 |TGL4 |MORE
Mutants/Phenotypes
Strains/Constructs
Jones L, et al. (2009) Cdc42p is activated during vacuole membrane fusion in a sterol-dependent subreaction of priming. J Biol Chem
       |CDC42 |ERG2 |ERG3 |ERG4 |ERG5 |RDI1 |SEC17 |SEC18 |STE20
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Muthusamy BP, et al. (2009) Control of protein and sterol trafficking by antagonistic activities of a type IV P-type ATPase and oxysterol binding protein homologue. Mol Biol Cell 20(12):2920-31
       |CPS1 |DNF1 |DNF2 |DNF3 |DRS2 |GGA1 |GGA2 |KES1 |PHO8 |PRC1 |SAC1 |SNC1
RNA Levels and Processing
Pedroso N, et al. (2009) Modulation of plasma membrane lipid profile and microdomains by H(2)O(2) in Saccharomyces cerevisiae. Free Radic Biol Med 46(2):289-98
       |ACC1 |ELO1 |ERG1 |ERG25 |ERG3 |ERG7 |FAS1 |FEN1 |GPT2 |LAC1 |LIP1 |OLE1 |RAD27 |SUR4
Reviews
Petrossian T and Clarke S (2009) Bioinformatic identification of novel methyltransferases Epigenomics 1(1):163-175
   |ABD1 |ABP140 |AML1 |BIO2 |BUD23 |CHO2 |COQ3 |COQ5 |CRG1 |CTM1 |DIM1 |DOT1 |DPH5 |ELP3 |MORE
Protein Sequence Features
Petrossian TC and Clarke SG (2009) Multiple Motif Scanning to identify methyltransferases from the yeast proteome. Mol Cell Proteomics 8(7):1516-26
       |COQ3 |COQ5 |CRG1 |MAM3 |OMS1 |PPM2 |RSM22 |SEE1 |SPE3 |SPE4 |TMT1 |TRM12 |TRM9 |TYW3 |MORE
Regulation of
Transcription
Rintala E, et al. (2009) Low oxygen levels as a trigger for enhancement of respiratory metabolism in Saccharomyces cerevisiae. BMC Genomics 10():461
       |AAT2 |ACO1 |ACS1 |ADH1 |ADH2 |AFR1 |AGA1 |AGA2 |ALD4 |ALD6 |ANT1 |ARE1 |ARN1 |ASH1 |MORE
Function/Process
Protein Processing/Modification/Regulation
Rossignol T, et al. (2009) The proteome of a wine yeast strain during fermentation, correlation with the transcriptome. J Appl Microbiol 107(1):47-55
       |ACT1 |ADE5,7 |ADH1 |AHP1 |ARO9 |ASC1 |ASN1 |CDC48 |CYS3 |EFT1 |ENO1 |ENO2 |FBA1 |GDH1 |MORE
Large-scale phenotype analysis
Tan SX, et al. (2009) Cu, Zn superoxide dismutase and NADP(H) homeostasis are required for tolerance of endoplasmic reticulum stress in Saccharomyces cerevisiae. Mol Biol Cell 20(5):1493-508
       |AAT2 |ADO1 |AIM26 |ALG7 |ARO2 |BCK1 |BUR2 |CCS1 |CNB1 |CNM67 |CRZ1 |CSG2 |ELP2 |ERG2 |MORE
Mutants/Phenotypes
Yoshikawa K, et al. (2009) Comprehensive phenotypic analysis for identification of genes affecting growth under ethanol stress in Saccharomyces cerevisiae. FEMS Yeast Res 9(1):32-44
       |ALD6 |ARO1 |ARO2 |ARO7 |COQ10 |COQ5 |COQ9 |COX11 |COX12 |COX14 |COX16 |COX18 |COX23 |COX7 |MORE
Cell Growth and Metabolism
Function/Process
Strains/Constructs
Zhang Z, et al. (2009) [Regulation role of sterol C-24 methyltransferase and sterol C-8 isomerase in the ergosterol biosynthesis of Saccharomyces cerevisiae] Wei Sheng Wu Xue Bao 49(8):1063-8
     |ERG2
Mutants/Phenotypes
Strains/Constructs
de Graaf B, et al. (2009) Cellular pathways for DNA repair and damage tolerance of formaldehyde-induced DNA-protein crosslinks. DNA Repair (Amst) 8(10):1207-14
       |ADH1 |ARP5 |BEM4 |CDC26 |CDC50 |CTF4 |DAL81 |ECM30 |ERG3 |ERG5 |LSM1 |MED1 |MGM101 |MMS22 |MORE
Mutants/Phenotypes
Folmer V, et al. (2008) H(2)O(2) induces rapid biophysical and permeability changes in the plasma membrane of Saccharomyces cerevisiae. Biochim Biophys Acta 1778(4):1141-7
     |ERG3
Function/Process
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Protein Sequence Features
Strains/Constructs
Ganapathy K, et al. (2008) Molecular probing of the Saccharomyces cerevisiae sterol 24-C methyltransferase reveals multiple amino acid residues involved with C(2)-transfer activity. Biochim Biophys Acta 1781(6-7):344-51

Mutants/Phenotypes
Strains/Constructs
Grossmann G, et al. (2008) Plasma membrane microdomains regulate turnover of transport proteins in yeast. J Cell Biol 183(6):1075-88
       |CAN1 |CAX4 |COG1 |ELP6 |ERG2 |ERG24 |ERG5 |FUR4 |FYV6 |GOS1 |HNT3 |MDG1 |MNN10 |MNN11 |MORE
Fungal Related Genes/Proteins
Iwaki T, et al. (2008) Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe. Microbiology 154(Pt 3):830-41
       |ERG2 |ERG3 |ERG4 |ERG5
Function/Process
Fungal Related Genes/Proteins
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Protein Physical Properties
Protein Sequence Features
Strains/Constructs
Substrates/Ligands/Cofactors
Jayasimha P and Nes WD (2008) Photoaffinity Labeling and Mutational Analysis of 24-C-Sterol Methyltransferase Defines the AdoMet Binding Site. Lipids 43(8):681-93

Genetic Interactions
Mutants/Phenotypes
Jin H, et al. (2008) Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast. J Cell Biol 180(4):813-26
       |ERG2 |ERG3 |LCB1 |PRM1 |STE5
Mutants/Phenotypes
Protein-protein Interactions
Strains/Constructs
Khoury CM, et al. (2008) A TSC22-like motif defines a novel antiapoptotic protein family. FEMS Yeast Res 8(4):540-63
       |ACA1 |ARR1 |CAD1 |CIN5 |CST6 |FYV10 |GCN4 |HAC1 |KCS1 |MCA1 |MET28 |NKP1 |RTG1 |RTG3 |MORE
Translational Regulation
Melamed D, et al. (2008) Yeast translational response to high salinity: global analysis reveals regulation at multiple levels. RNA 14(7):1337-51
       |ADK1 |AIM17 |ALD3 |AQR1 |ARG2 |ARG3 |ARG5,6 |ARG7 |ARG80 |ARG81 |ARG82 |AST1 |BCY1 |BTN2 |MORE
Function/Process
Mutants/Phenotypes
Protein Physical Properties
Protein Processing/Modification/Regulation
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Substrates/Ligands/Cofactors
Nes WD, et al. (2008) Yeast sterol C24-methyltransferase: role of highly conserved tyrosine-81 in catalytic competence studied by site-directed mutagenesis and thermodynamic analysis. Arch Biochem Biophys 477(2):313-23

Mutants/Phenotypes
Strains/Constructs
Rojas M, et al. (2008) Genomewide expression profiling of cryptolepine-induced toxicity in Saccharomyces cerevisiae. Antimicrob Agents Chemother 52(11):3844-50
       |AFT1 |ALD3 |ARN1 |ARN2 |CCP1 |CDC19 |CIS3 |CTT1 |DAN1 |ECM33 |EGT2 |ENO2 |ERV1 |EXG2 |MORE
Mutants/Phenotypes
Strains/Constructs
Ruotolo R, et al. (2008) Membrane transporters and protein traffic networks differentially affecting metal tolerance: a genomic phenotyping study in yeast. Genome Biol 9(4):R67
       |AAT2 |AFT1 |ALF1 |APL5 |APL6 |APM3 |APS3 |ARO2 |BCK1 |BUB3 |CCC2 |CCR4 |CDC10 |CLC1 |MORE
Mutants/Phenotypes
Strains/Constructs
Serero A, et al. (2008) Yeast genes involved in cadmium tolerance: Identification of DNA replication as a target of cadmium toxicity. DNA Repair (Amst) 7(8):1262-75
       |ADA2 |ARP5 |ATP1 |BRO1 |BUR2 |CCR4 |CCS1 |CTF4 |CTR1 |CUP2 |CYS4 |DID4 |DNA2 |DOA4 |MORE
Mutants/Phenotypes
Shima J, et al. (2008) Possible roles of vacuolar H(+)-ATPase and mitochondrial function in tolerance to air-drying stress revealed by genome-wide screening of Saccharomyces cerevisiae deletion strains. Yeast 25(3):179-90
       |ADA2 |ADK1 |ADO1 |AEP2 |AFG3 |ANP1 |APQ13 |ARF1 |ARG82 |ARV1 |ATG17 |ATP17 |BDF1 |BEM1 |MORE
Cellular Location
Other Features
Strains/Constructs
Takeda Y and Nakano A (2008) In vitro Formation of a Novel Type of Membrane Vesicles Containing Dpm1p: Putative Transport Vesicles for Lipid Droplets in Budding Yeast. J Biochem 143(6):803-11
     |DPM1
Mutants/Phenotypes
Strains/Constructs
Tang F, et al. (2008) A life-span extending form of autophagy employs the vacuole-vacuole fusion machinery. Autophagy 4(7):874-86
       |ATG1 |ATG10 |ATG11 |ATG15 |ATG17 |ATG22 |ATG7 |ATG8 |AVT3 |AVT4 |ERG2 |ERG28 |ERG5 |GSG1 |MORE
Mutants/Phenotypes
Ulanovskaya OA, et al. (2008) Synthesis enables identification of the cellular target of leucascandrolide A and neopeltolide. Nat Chem Biol 4(7):418-24
       |ATG11 |COB |COR1 |CYT1 |ERG3 |HOM3 |KCS1 |PPA1 |QCR10 |QCR2 |QCR6 |QCR7 |QCR8 |QCR9 |MORE
Cellular Location
Strains/Constructs
Vorvis C, et al. (2008) Photoactivatable GFP tagging cassettes for protein-tracking studies in the budding yeast Saccharomyces cerevisiae. Yeast 25(9):651-9
       |NUM1
Genetic Interactions
Infection and Antifungals
Mutants/Phenotypes
Strains/Constructs
Welscher YM, et al. (2008) Natamycin Blocks Fungal Growth by Binding Specifically to Ergosterol without Permeabilizing the Membrane. J Biol Chem 283(10):6393-401
       |ERG2 |ERG3 |ERG4 |ERG5
Cross-species Expression
Disease Gene Related
Mutants/Phenotypes
Strains/Constructs
Zabrocki P, et al. (2008) Phosphorylation, lipid raft interaction and traffic of alpha-synuclein in a yeast model for Parkinson. Biochim Biophys Acta 1783(10):1767-80
       |CKA1 |CKA2 |CKB1 |CKB2 |DOA4 |ERG24 |ERG28 |MAK3 |MDM20 |NAT3 |PEP12 |PKH1 |PKH2 |RVS161 |MORE
Mutants/Phenotypes
Strains/Constructs
Zhang M, et al. (2008) Deletion of yeast CWP genes enhances cell permeability to genotoxic agents. Toxicol Sci 103(1):68-76
       |CWP1 |CWP2 |PDR5
Mutants/Phenotypes
Burgis NE and Samson LD (2007) The Protein Degradation Response of Saccharomyces cerevisiae to Classical DNA-Damaging Agents. Chem Res Toxicol 20(12):1843-53
       |MAG1 |MEC1 |PEP3 |RAD23 |RAD6 |RPN10 |RPN4 |UBI4 |VAM3 |YDJ1
Mutants/Phenotypes
Strains/Constructs
Daicho K, et al. (2007) The ergosterol biosynthesis inhibitor zaragozic acid promotes vacuolar degradation of the tryptophan permease Tat2p in yeast. Biochim Biophys Acta 1768(7):1681-1690
       |ERG1 |ERG11 |ERG9 |PEP12 |PEP4 |RSP5 |TAT2 |VPS1 |VPS27 |VPS45
Mutants/Phenotypes
Fairn GD, et al. (2007) A chemogenomic screen in Saccharomyces cerevisiae uncovers a primary role for the mitochondria in farnesol toxicity and its regulation by the Pkc1 pathway. J Biol Chem 282(7):4868-74
         |AIM21 |ARG4 |BCK1 |CBP1 |CLA4 |COR1 |COX11 |DAL5 |ECM33 |EOS1 |FSF1 |FUI1 |FUR4 |FYV6 |MORE
Mutants/Phenotypes
Strains/Constructs
Ito-Harashima S, et al. (2007) Translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast. Genes Dev 21(5):519-24

Mutants/Phenotypes
Strains/Constructs
Pagani MA, et al. (2007) Disruption of iron homeostasis in Saccharomyces cerevisiae by high zinc levels: a genome-wide study. Mol Microbiol 65(2):521-37
       |ACO1 |ACO2 |ADE1 |ADE12 |ADE13 |ADE17 |ADE2 |ADE4 |ADE5,7 |ADE6 |ADK1 |AFT1 |AKR1 |ARN1 |MORE
Reviews
Schulz TA and Prinz WA (2007) Sterol transport in yeast and the oxysterol binding protein homologue (OSH) family. Biochim Biophys Acta 1771(6):769-80
       |AUS1 |CAT5 |DAN1 |ERG1 |ERG11 |ERG27 |ERG3 |ERG5 |ERG7 |HES1 |KES1 |MGM101 |NCR1 |NPC2 |MORE
Genetic Interactions
Strains/Constructs
Shah Alam Bhuiyan M, et al. (2007) Synthetically lethal interactions involving loss of the yeast ERG24: the sterol C-14 reductase gene. Lipids 42(1):69-76
         |ERG2 |ERG24 |ERG28 |ERG3 |SUR4
Large-scale phenotype analysis
Yadav J, et al. (2007) A phenomics approach in yeast links proton and calcium pump function in the Golgi. Mol Biol Cell 18(4):1480-9
           |CUP5 |ERG2 |ERG3 |ERG4 |GAL11 |GCN4 |GCN5 |HFI1 |NHP10 |OPI1 |PMR1 |RPN4 |SAC1 |SET3 |MORE
Mutants/Phenotypes
Strains/Constructs
Das-Bradoo S, et al. (2006) Interaction between PCNA and diubiquitinated Mcm10 is essential for cell growth in budding yeast. Mol Cell Biol 26(13):4806-17
       |MCM10 |POL1 |POL30
Mutants/Phenotypes
Techniques and Reagents
Freimoser FM, et al. (2006) Systematic screening of polyphosphate (poly P) levels in yeast mutant cells reveals strong interdependence with primary metabolism. Genome Biol 7(11):R109
       |ADE1 |ADE3 |AKR1 |ALT2 |APL5 |APL6 |APM3 |APS3 |ARO1 |ARP5 |ATG12 |ATG20 |ATP15 |AVT6 |MORE
Function/Process
Mutants/Phenotypes
Strains/Constructs
Grossmann G, et al. (2006) Lipid raft-based membrane compartmentation of a plant transport protein expressed in Saccharomyces cerevisiae. Eukaryot Cell 5(6):945-53

Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Okamoto M, et al. (2006) Glycosylphosphatidylinositol-anchored proteins are required for the transport of detergent-resistant microdomain-associated membrane proteins Tat2p and Fur4p. J Biol Chem 281(7):4013-23
       |BUL1 |EAF3 |FUR4 |GWT1 |SSB1 |TAT2 |UBP5 |YDR266C
Mutants/Phenotypes
Rand JD and Grant CM (2006) The thioredoxin system protects ribosomes against stress-induced aggregation. Mol Biol Cell 17(1):387-401
       |AAT2 |ADH1 |ADK1 |AFR1 |AKR1 |ANP1 |APQ13 |ARO2 |ARP8 |ARV1 |ARX1 |ATP12 |ATP2 |BEM1 |MORE
Cellular Location
Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
           |AAC1 |AAC3 |AAT1 |ABC1 |ABF2 |ACC1 |ACH1 |ACK1 |ACN9 |ACO1 |ACO2 |ACP1 |ACS1 |ADH3 |MORE
Mutants/Phenotypes
Strains/Constructs
Sharma SC (2006) Implications of sterol structure for membrane lipid composition, fluidity and phospholipid asymmetry in Saccharomyces cerevisiae. FEMS Yeast Res 6(7):1047-51
       |ERG2 |ERG3
Function/Process
Mutants/Phenotypes
Strains/Constructs
Shobayashi M, et al. (2006) A new method for isolation of S-adenosylmethionine (SAM)-accumulating yeast. Appl Microbiol Biotechnol 69(6):704-10
       |ERG4
Mutants/Phenotypes
Strains/Constructs
Simons V, et al. (2006) Dual effects of plant steroidal alkaloids on Saccharomyces cerevisiae. Antimicrob Agents Chemother 50(8):2732-40
         |ERG2 |ERG3
Protein Processing/Modification/Regulation
Techniques and Reagents
Tagwerker C, et al. (2006) A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking. Mol Cell Proteomics 5(4):737-48
         |AAC1 |AAC3 |ACB1 |ACC1 |ACS2 |ADE3 |ADE5,7 |ADE6 |ADH4 |ADO1 |AGP1 |AHA1 |AHP1 |ALA1 |MORE
Genetic Interactions
Mutants/Phenotypes
Valachovic M, et al. (2006) Cumulative mutations affecting sterol biosynthesis in the yeast Saccharomyces cerevisiae result in synthetic lethality that is suppressed by alterations in sphingolipid profiles. Genetics 173(4):1893-908
       |BCK2 |ECM22 |ERG2 |ERG28 |ERG4 |ERG5 |GDA1 |HAP1 |HDA3 |IES1 |RDN25-1 |RDN37-1 |RPN9 |SSN2 |MORE
Transcription
Warringer J and Blomberg A (2006) Involvement of yeast YOL151W/GRE2 in ergosterol metabolism. Yeast 23(5):389-98
       |BGL2 |ENO1 |ERG10 |ERG11 |ERG2 |ERG24 |GRE2 |HMG1 |HMG2 |HXK1 |MVD1 |RNR4 |TDH1
Cellular Location
Zahedi RP, et al. (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50
         |AFG1 |AIM18 |ALD4 |ALO1 |ATP15 |ATP2 |ATP3 |ATP5 |AYR1 |BNA4 |CBR1 |CIR1 |COR1 |CYB2 |MORE
Non-Fungal Related Genes/Proteins
Zhou W, et al. (2006) Mechanistic analysis of a multiple product sterol methyltransferase implicated in ergosterol biosynthesis in Trypanosoma brucei. J Biol Chem 281(10):6290-6

Mutants/Phenotypes
van Voorst F, et al. (2006) Genome-wide identification of genes required for growth of Saccharomyces cerevisiae under ethanol stress. Yeast 23(5):351-9
       |ASR1 |ATP1 |BEM2 |FAB1 |FEN1 |GCN5 |GIM4 |GIM5 |HMI1 |IMG1 |KAR3 |MSK1 |MSN2 |MTF2 |MORE
Infection and Antifungals
Mutants/Phenotypes
Strains/Constructs
Cowen LE and Lindquist S (2005) Hsp90 potentiates the rapid evolution of new traits: drug resistance in diverse fungi. Science 309(5744):2185-9
       |CKA2 |CNA1 |CNB1 |CPR1 |ERG3 |HSC82 |LCL1 |PDR1 |PDR5 |RTC2 |SCS2 |YGR283C |YLR407W |YMR099C |MORE
Reviews
Henneberry AL and Sturley SL (2005) Sterol homeostasis in the budding yeast, Saccharomyces cerevisiae. Semin Cell Dev Biol 16(2):155-61
         |NCR1
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Kishimoto T, et al. (2005) Defects in structural integrity of ergosterol and the Cdc50p-Drs2p putative phospholipid translocase cause accumulation of endocytic membranes, onto which actin patches are assembled in yeast. Mol Biol Cell 16(12):5592-609
       |ABP1 |ACT1 |BNI1 |CDC42 |CDC50 |DRS2 |ERG2 |ERG3 |ERG4 |ERG5 |GIC1 |LAS17 |SLA2 |SNC1
RNA Levels and Processing
Regulation of
Lai LC, et al. (2005) Dynamical remodeling of the transcriptome during short-term anaerobiosis in Saccharomyces cerevisiae: differential response and role of Msn2 and/or Msn4 and other factors in galactose and glucose media. Mol Cell Biol 25(10):4075-91
         |AAC1 |ADE1 |ADE12 |ADE17 |ADE4 |AFR1 |AIM17 |AIM3 |AKR1 |AKR2 |ALA1 |ALD5 |ARE1 |ARN1 |MORE
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Liu Z, et al. (2005) A novel degron-mediated degradation of the RTG pathway regulator, Mks1p, by SCFGrr1. Mol Biol Cell 16(10):4893-904
       |BMH1 |GRR1 |MKS1 |RTG2
Protein-protein Interactions
Techniques and Reagents
Mo C and Bard M (2005) A systematic study of yeast sterol biosynthetic protein-protein interactions using the split-ubiquitin system. Biochim Biophys Acta 1737(2-3):152-60
       |ERG1 |ERG11 |ERG2 |ERG24 |ERG25 |ERG26 |ERG27 |ERG28 |ERG3 |ERG4 |ERG5 |ERG7 |ERG9
Protein-protein Interactions
Mo C and Bard M (2005) Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex. J Lipid Res 46(9):1991-8
       |ERG1 |ERG11 |ERG25 |ERG26 |ERG27 |ERG28
Mutants/Phenotypes
Ott RG, et al. (2005) Flux of sterol intermediates in a yeast strain deleted of the lanosterol C-14 demethylase Erg11p. Biochim Biophys Acta 1735(2):111-8
       |ERG11 |ERG3 |ERG7
Function/Process
Mutants/Phenotypes
Strains/Constructs
Proszynski TJ, et al. (2005) A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast. Proc Natl Acad Sci U S A 102(50):17981-6
       |AYR1 |BUD27 |CCZ1 |CHS5 |ERG4 |FAB1 |GIM3 |KES1 |LCB1 |MCH5 |MON1 |OPI9 |PAC10 |RVS161 |MORE
Mutants/Phenotypes
Sano T, et al. (2005) Regulation of the sphingoid long-chain base kinase Lcb4p by ergosterol and heme: studies in phytosphingosine-resistant mutants. J Biol Chem 280(44):36674-82
       |DPL1 |ERG2 |ERG3 |ERG4 |ERG5 |HAP1 |HEM14 |HMG1 |KES1 |LCB3 |LCB4 |PBP1 |PDR5 |TPS1 |MORE
RNA Levels and Processing
Shobayashi M, et al. (2005) Effects of Culture Conditions on Ergosterol Biosynthesis by Saccharomyces cerevisiae. Biosci Biotechnol Biochem 69(12):2381-8
       |ERG13 |ERG2 |ERG20 |ERG24 |ERG26 |ERG28 |ERG3 |ERG5 |HMG1
Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
Walsh L, et al. (2005) Genetic modification and variations in solvent increase the sensitivity of the yeast RAD54-GFP genotoxicity assay. Mutagenesis 20(5):317-27
         |PDR5 |RAD54 |SNQ2 |YOR1
Function/Process
Mutants/Phenotypes
Strains/Constructs
Branco MR, et al. (2004) Decrease of H2O2 plasma membrane permeability during adaptation to H2O2 in Saccharomyces cerevisiae. J Biol Chem 279(8):6501-6
         |CCP1 |CTT1 |ERG3
Mutants/Phenotypes
Strains/Constructs
Chang HJ, et al. (2004) Role of the unfolded protein response pathway in secretory stress and regulation of INO1 expression in Saccharomyces cerevisiae. Genetics 168(4):1899-913
       |HAC1 |INO1 |IRE1
RNA Levels and Processing
Regulation of
Jones DL, et al. (2004) Genome-Wide Analysis of the Effects of Heat Shock on a Saccharomyces cerevisiae Mutant With a Constitutively Activated cAMP-Dependent Pathway. Comp Funct Genomics 5(5):419-31
               |BTN2 |COX6 |DCS1 |ENO1 |ERG10 |ERG11 |ERG12 |ERG13 |ERG2 |ERG20 |ERG24 |ERG26 |ERG27 |ERG3 |MORE
RNA Levels and Processing
Techniques and Reagents
Krantz M, et al. (2004) Anaerobicity prepares Saccharomyces cerevisiae cells for faster adaptation to osmotic shock. Eukaryot Cell 3(6):1381-90
           |ALD2 |ALD3 |ALD4 |ALD6 |CTT1 |ERG1 |ERG10 |ERG11 |ERG12 |ERG13 |ERG2 |ERG20 |ERG24 |ERG25 |MORE
Function/Process
Mutants/Phenotypes
Strains/Constructs
Markovich S, et al. (2004) Genomic approach to identification of mutations affecting caspofungin susceptibility in Saccharomyces cerevisiae. Antimicrob Agents Chemother 48(10):3871-6
       |BCK1 |CCR4 |CHC1 |CHS3 |CHS7 |CKA2 |CSG2 |ERG3 |ERG5 |FKS1 |ILM1 |MID2 |MNN10 |NPL3 |MORE
Function/Process
Protein-protein Interactions
Strains/Constructs
Mo C, et al. (2004) The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p). Biochim Biophys Acta 1686(1-2):30-6
       |ERG11 |ERG28 |ERG3 |ERG7
Cellular Location
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Mullner H, et al. (2004) Targeting of proteins involved in sterol biosynthesis to lipid particles of the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 1663(1-2):9-13
       |ERG1 |ERG7
Mutants/Phenotypes
Pannunzio VG, et al. (2004) A Simple Chemical Method for Rendering Wild-Type Yeast Permeable to Brefeldin A That Does Not Require the Presence of an erg6 Mutation. J Biomed Biotechnol 2004(3):150-155

Genomic expression study
RNA Levels and Processing
Regulation of
Parveen M, et al. (2004) Response of Saccharomyces cerevisiae to a monoterpene: evaluation of antifungal potential by DNA microarray analysis. J Antimicrob Chemother 54(1):46-55
               |AAD6 |ADH5 |ADH7 |ADY2 |AGA2 |AMS1 |ARG1 |ARN2 |ATF2 |ATX1 |BCK1 |BFR1 |BSC1 |BUD7 |MORE
Cross-species Expression
Function/Process
Genetic Interactions
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Pourshafie M, et al. (2004) Cloning of S-adenosyl-L-methionine:C-24-Delta-sterol-methyltransferase (ERG6) from Leishmania donovani and characterization of mRNAs in wild-type and amphotericin B-Resistant promastigotes. Antimicrob Agents Chemother 48(7):2409-14

Mutants/Phenotypes
Serrano R, et al. (2004) Copper and iron are the limiting factors for growth of the yeast Saccharomyces cerevisiae in an alkaline environment. J Biol Chem 279(19):19698-704
           |AFT1 |ATX1 |BEM1 |BSD2 |BUD25 |CCC2 |CCS1 |COX17 |CTR1 |CUP5 |DIA2 |ERG2 |FET3 |FET4 |MORE
Cellular Location
Genetic Interactions
Sorger D, et al. (2004) A yeast strain lacking lipid particles bears a defect in ergosterol formation. J Biol Chem 279(30):31190-6
       |ARE1 |ARE2 |AYR1 |DGA1 |ERG1 |ERG7 |LRO1
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Tedrick K, et al. (2004) Enhanced membrane fusion in sterol-enriched vacuoles bypasses the Vrp1p requirement. Mol Biol Cell 15(10):4609-21
         |ACT1 |ARC35 |HUL4 |LAS17 |MIG1 |MKK1 |RRN5 |TOS2 |VAM3 |VPS33 |VPS5 |VRP1 |YPT7
Regulation of
Transcription
Agarwal AK, et al. (2003) Genome-wide expression profiling of the response to polyene, pyrimidine, azole, and echinocandin antifungal agents in Saccharomyces cerevisiae. J Biol Chem 278(37):34998-5015
         |AGA1 |AMS1 |ATF2 |AUS1 |BAG7 |BAP2 |BAP3 |CRH1 |CTR1 |CWP1 |CYB5 |DAN1 |DAN4 |ELO1 |MORE
Function/Process
Mutants/Phenotypes
Strains/Constructs
Anderson JB, et al. (2003) Mode of selection and experimental evolution of antifungal drug resistance in Saccharomyces cerevisiae. Genetics 163(4):1287-98
         |CKA2 |ERG11 |ERG28 |ERG3 |LCL1 |PDR1 |PDR5 |RTC2 |SCS2 |SML1 |SNQ2 |SWH1 |YGR283C |YLR407W |MORE
Function/Process
Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
Clark DD and Peterson BR (2003) Analysis of protein tyrosine kinase inhibitors in recombinant yeast lacking the ERG6 gene. Chembiochem 4(1):101-7

Function/Process
Mutants/Phenotypes
Strains/Constructs
Gupta SS, et al. (2003) Antifungal activity of amiodarone is mediated by disruption of calcium homeostasis. J Biol Chem 278(31):28831-9
         |CCH1 |CUP5 |ERG24 |ERG3 |MID1 |PDR5 |PMC1 |PMR1 |PTC1 |RCY1 |SSC1 |VCX1 |VPS45 |YPK1 |MORE
Cellular Location
Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
         |AAC1 |AAC3 |AAT1 |ABC1 |ABF2 |ACC1 |ACH1 |ACK1 |ACO1 |ACO2 |ACP1 |ACS1 |ADH3 |ADK2 |MORE
Function/Process
Mutants/Phenotypes
Strains/Constructs
Umebayashi K and Nakano A (2003) Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J Cell Biol 161(6):1117-31
     |TAT2
Function/Process
Mutants/Phenotypes
Strains/Constructs
Valdez-Taubas J and Pelham HR (2003) Slow diffusion of proteins in the yeast plasma membrane allows polarity to be maintained by endocytic cycling. Curr Biol 13(18):1636-40
       |ACT1 |SNC1 |SSO1 |YBR016W
Fungal Related Genes/Proteins
Young LY, et al. (2003) Disruption of ergosterol biosynthesis confers resistance to amphotericin B in Candida lusitaniae. Antimicrob Agents Chemother 47(9):2717-24
     |ERG3
Regulation of
Transcription
Zhang W, et al. (2003) Microarray analyses of the metabolic responses of Saccharomyces cerevisiae to organic solvent dimethyl sulfoxide. J Ind Microbiol Biotechnol 30(1):57-69
         |AAH1 |ABP140 |ACS2 |ADE1 |ADE12 |ADE17 |ADE4 |ADE8 |ADH2 |ADH4 |ADH5 |ADK1 |ADO1 |ALD3 |MORE
Function/Process
Mutants/Phenotypes
Strains/Constructs
Bagnat M and Simons K (2002) Cell surface polarization during yeast mating. Proc Natl Acad Sci U S A 99(22):14183-8
       |BUL1 |BUL2 |FIG1 |FUS1 |FUS2 |GAP1 |HXT2 |LCB1 |LCB3 |PRM1 |SHO1 |STE6 |TRE1
Mutants/Phenotypes
Strains/Constructs
Desmoucelles C, et al. (2002) Screening the yeast "disruptome" for mutants affecting resistance to the immunosuppressive drug, mycophenolic acid. J Biol Chem 277(30):27036-44
       |ANP1 |BUD30 |CTK1 |CTK3 |DAL81 |DST1 |ERG24 |ERG3 |ERG4 |ERG5 |GCN5 |HOM2 |HPR1 |HTZ1 |MORE
Function/Process
Mutants/Phenotypes
Dimmer KS, et al. (2002) Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae. Mol Biol Cell 13(3):847-53
       |ABF2 |ACO1 |ADA2 |ADK1 |AEP1 |AEP2 |AEP3 |AFG3 |AFT1 |AIM10 |AIM22 |ALY1 |APQ13 |ARG82 |MORE
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Eisenkolb M, et al. (2002) A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast. Mol Biol Cell 13(12):4414-28
         |GAS1 |PMA1 |SUR4
Function/Process
Emter R, et al. (2002) ERG6 and PDR5 regulate small lipophilic drug accumulation in yeast cells via distinct mechanisms. FEBS Lett 521(1-3):57-61
       |PDR5
Function/Process
Regulation of
Transcription
Hongay C, et al. (2002) Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae. EMBO J 21(15):4114-24
       |ERG2 |ERG9 |MOT3 |PAN1 |VPS41
Mutants/Phenotypes
Strains/Constructs
Marin S, et al. (2002) Promoter-specific inhibition of transcription by daunorubicin in Saccharomyces cerevisiae. Biochem J 368(Pt 1):131-6
       |GAL4 |PDR1 |PDR3 |PDR5
Function/Process
Mutants/Phenotypes
Protein Physical Properties
Protein Sequence Features
Strains/Constructs
Substrates/Ligands/Cofactors
Nes WD, et al. (2002) Active site mapping and substrate channeling in the sterol methyltransferase pathway. J Biol Chem 277(45):42549-56

Function/Process
Mutants/Phenotypes
Plowright AT, et al. (2002) Transcriptional response pathways in a yeast strain sensitive to saframycin a and a more potent analog: evidence for a common basis of activity. Chem Biol 9(5):607-18
           |PDR1 |PDR3
Function/Process
Mutants/Phenotypes
Strains/Constructs
Kato M and Wickner W (2001) Ergosterol is required for the Sec18/ATP-dependent priming step of homotypic vacuole fusion. EMBO J 20(15):4035-40
       |ERG3 |ERG4 |ERG5 |SEC17 |SEC18
Cellular Location
Function/Process
Pichler H, et al. (2001) A subfraction of the yeast endoplasmic reticulum associates with the plasma membrane and has a high capacity to synthesize lipids. Eur J Biochem 268(8):2351-61
       |ERG1 |ERG9
Techniques and Reagents
Sato M, et al. (2001) Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis. Genes Cells 6(6):495-506
       |RER2 |SRT1
Alias
Shieh HL, et al. (2001) Biochemical analysis of fructose-1,6-bisphosphatase import into vacuole import and degradation vesicles reveals a role for UBC1 in vesicle biogenesis. J Biol Chem 276(13):10398-406
       |FBP1 |RPL40A |RPL40B |RPS31 |UBC1 |UBI4 |VID24
Mutants/Phenotypes
Strains/Constructs
Sievi E, et al. (2001) Proteolytic function of GPI-anchored plasma membrane protease Yps1p in the yeast vacuole and Golgi. Traffic 2(12):896-907
       |HSP150 |PEP4 |SEC7 |YPS1
Mutants/Phenotypes
Strains/Constructs
Bammert GF and Fostel JM (2000) Genome-wide expression patterns in Saccharomyces cerevisiae: comparison of drug treatments and genetic alterations affecting biosynthesis of ergosterol. Antimicrob Agents Chemother 44(5):1255-65
       |ERG2 |ERG25 |ERG5 |RGI1 |TOS6
Mutants/Phenotypes
Strains/Constructs
Inoue T, et al. (2000) Cloning and characterization of a gene complementing the mutation of an ethanol-sensitive mutant of sake yeast. Biosci Biotechnol Biochem 64(2):229-36

Reviews
Nes WD (2000) Sterol methyl transferase: enzymology and inhibition. Biochim Biophys Acta 1529(1-3):63-88

Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Sitcheran R, et al. (2000) A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae. Genetics 156(3):963-72
       |LEM3 |PDR5
Reviews
Sturley SL (2000) Conservation of eukaryotic sterol homeostasis: new insights from studies in budding yeast. Biochim Biophys Acta 1529(1-3):155-63
       |ARE1 |ARE2 |ARH1 |ARV1 |ECM22 |ERG10 |ERG11 |ERG9 |HMG1 |HMG2 |HMS1 |LRO1 |NCR1 |ROX1 |MORE
Mutants/Phenotypes
Zweytick D, et al. (2000) Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, erg4p, from the yeast saccharomyces cerevisiae. FEBS Lett 470(1):83-7
       |ERG4
Reviews
Zweytick D, et al. (2000) Intracellular lipid particles of eukaryotic cells. Biochim Biophys Acta 1469(2):101-20
       |AYR1 |EHT1 |ERG1 |ERG7 |FAA1 |FAA4 |FAT1 |NUS1 |PET10 |SLC1 |TDH1 |TDH2 |TDH3 |TGL1 |MORE
Cellular Location
Athenstaedt K, et al. (1999) Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae. J Bacteriol 181(20):6441-8
       |EHT1 |ERG1 |ERG7 |FAA1 |FAA4 |FAT1 |NUS1 |PET10 |TGL1 |TGL3 |YJU3 |YOR059C
Genomic expression study
Regulation of
Dimster-Denk D, et al. (1999) Comprehensive evaluation of isoprenoid biosynthesis regulation in Saccharomyces cerevisiae utilizing the Genome Reporter Matrix. J Lipid Res 40(5):850-60
       |ARE1 |ARE2 |BEM1 |BET2 |BET4 |BTS1 |CAT5 |CDC43 |COQ1 |COQ2 |COQ3 |COQ6 |COX10 |ERG1 |MORE
Mutants/Phenotypes
Strains/Constructs
Kaur R and Bachhawat AK (1999) The yeast multidrug resistance pump, Pdr5p, confers reduced drug resistance in erg mutants of Saccharomyces cerevisiae. Microbiology 145 ( Pt 4)():809-18
       |CHO1 |ERG2 |ERG3 |ERG4 |PDR5
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Munn AL, et al. (1999) Specific sterols required for the internalization step of endocytosis in yeast. Mol Biol Cell 10(11):3943-57
       |ERG2
Non-Fungal Related Genes/Proteins
Protein Sequence Features
Niewmierzycka A and Clarke S (1999) S-Adenosylmethionine-dependent methylation in Saccharomyces cerevisiae. Identification of a novel protein arginine methyltransferase. J Biol Chem 274(2):814-24
       |ABD1 |ABP140 |BDH2 |BUD23 |COQ3 |COQ5 |COX1 |CRG1 |DIM1 |GCD14 |HMT1 |MET1 |MNI1 |MTQ2 |MORE
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Chabane S, et al. (1998) Over-expression of the yeast BFR2 gene partially suppresses the growth defects induced by Brefeldin A and by four ER-to-Golgi mutations. Curr Genet 33(1):21-8
       |BFR2 |SEC13 |SEC16 |SEC23 |YPT1
Reviews
Daum G, et al. (1998) Biochemistry, cell biology and molecular biology of lipids of Saccharomyces cerevisiae. Yeast 14(16):1471-510
       |ACC1 |ACP1 |AUR1 |CDS1 |CEM1 |CHO1 |CHO2 |CKI1 |CPT1 |CRD1 |CSG2 |DPL1 |DPP1 |EPT1 |MORE
Genetic Interactions
Strains/Constructs
Jensen-Pergakes KL, et al. (1998) Sequencing, disruption, and characterization of the Candida albicans sterol methyltransferase (ERG6) gene: drug susceptibility studies in erg6 mutants. Antimicrob Agents Chemother 42(5):1160-7

Cellular Location
Leber R, et al. (1998) Dual localization of squalene epoxidase, Erg1p, in yeast reflects a relationship between the endoplasmic reticulum and lipid particles. Mol Biol Cell 9(2):375-86
       |ERG1 |KAR2
Function/Process
Protein Physical Properties
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Techniques and Reagents
Nes WD, et al. (1998) Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine: delta 24(25)- to delta 24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae. Arch Biochem Biophys 353(2):297-311

Function/Process
Substrates/Ligands/Cofactors
Acuna-Johnson AP, et al. (1997) Stereochemistry of yeast delta 24-sterol methyl transferase. Bioorg Med Chem 5(5):821-32

Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Gachotte D, et al. (1997) A yeast sterol auxotroph (erg25) is rescued by addition of azole antifungals and reduced levels of heme. Proc Natl Acad Sci U S A 94(21):11173-8
       |ERG11 |ERG25 |HEM2 |HEM4
Cross-species Expression
Non-Fungal Related Genes/Proteins
Strains/Constructs
Grebenok RJ, et al. (1997) Characterization of Zea mays endosperm C-24 sterol methyltransferase: one of two types of sterol methyltransferase in higher plants. Plant Mol Biol 34(6):891-6

Function/Process
Protein Processing/Modification/Regulation
Protein-protein Interactions
Nes WD, et al. (1997) Substrate-based inhibitors of the (S)-adenosyl-L-methionine:delta24(25)- to delta24(28)-sterol methyl transferase from Saccharomyces cerevisiae. Arch Biochem Biophys 342(1):68-81

Genetic Interactions
Mutants/Phenotypes
Regulatory Role
Strains/Constructs
Arthington-Skaggs BA, et al. (1996) Positive and negative regulation of a sterol biosynthetic gene (ERG3) in the post-squalene portion of the yeast ergosterol pathway. FEBS Lett 392(2):161-5
       |ARE1 |ARE2 |ERG2 |ERG3 |ERG4 |ERG5
Genetic Interactions
Fang M, et al. (1996) Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis. EMBO J 15(23):6447-59
     |ERG3 |HES1 |HMG1 |HMG2 |KES1 |SEC14 |SWH1
Function/Process
Genetic Interactions
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Hemenway CS and Heitman J (1996) Immunosuppressant target protein FKBP12 is required for P-glycoprotein function in yeast. J Biol Chem 271(31):18527-34
       |CNB1 |CPR1 |FPR1 |RAD52 |STE6 |VMA22
Alias
Function/Process
Mutants/Phenotypes
Strains/Constructs
Hoffman M and Chiang HL (1996) Isolation of degradation-deficient mutants defective in the targeting of fructose-1,6-bisphosphatase into the vacuole for degradation in Saccharomyces cerevisiae. Genetics 143(4):1555-66
     |FBP1
Non-Fungal Related Genes/Proteins
Strains/Constructs
Husselstein T, et al. (1996) Transformation of Saccharomyces cerevisiae with a cDNA encoding a sterol C-methyltransferase from Arabidopsis thaliana results in the synthesis of 24-ethyl sterols. FEBS Lett 381(1-2):87-92

Alias
Function/Process
Mutants/Phenotypes
Strains/Constructs
Lee DH and Goldberg AL (1996) Selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in Saccharomyces cerevisiae. J Biol Chem 271(44):27280-4

Function/Process
Protein Physical Properties
Substrates/Ligands/Cofactors
Venkatramesh M, et al. (1996) Mechanism and structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase from Saccharomyces cerevisiae. Biochim Biophys Acta 1299(3):313-24
     |ERG7
Regulation of
Substrates/Ligands/Cofactors
Techniques and Reagents
Venkatramesh M, et al. (1996) Sterol specificity of the Saccharomyces cerevisiae ERG6 gene product expressed in Escherichia coli. Lipids 31(4):373-7

Mutants/Phenotypes
Strains/Constructs
Espinet C, et al. (1995) An efficient method to isolate yeast genes causing overexpression-mediated growth arrest. Yeast 11(1):25-32
     |ACT1 |AFT1 |ARF2 |ATE1 |AUA1 |HSF1 |MCM1 |NHP6A |NSR1 |NTH1 |RHO1 |SEC17 |SHE1 |SHE10 |MORE
Reviews
Lees ND, et al. (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
     |ERG1 |ERG11 |ERG2 |ERG24 |ERG3 |ERG4 |ERG5 |ERG7 |ERG9 |FEN1 |FEN2
Reviews
Parks LW and Casey WM (1995) Physiological implications of sterol biosynthesis in yeast. Annu Rev Microbiol 49:95-116
       |ERG1 |ERG10 |ERG11 |ERG12 |ERG13 |ERG2 |ERG20 |ERG24 |ERG25 |ERG3 |ERG4 |ERG5 |ERG7 |ERG8 |MORE
Reviews
Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
     |ERG1 |ERG10 |ERG11 |ERG12 |ERG2 |ERG20 |ERG24 |ERG3 |ERG4 |ERG5 |ERG7 |ERG8 |ERG9 |HMG1 |MORE
Cell Cycle Phase Involved
Mutants/Phenotypes
Strains/Constructs
Prendergast JA, et al. (1995) Mutations sensitizing yeast cells to the start inhibitor nalidixic acid. Yeast 11(6):537-47
     |ARO7 |ERG3
Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
Rambourg A, et al. (1995) Effects of brefeldin A on the three-dimensional structure of the Golgi apparatus in a sensitive strain of Saccharomyces cerevisiae. Anat Rec 241(1):1-9
     |ARF1
Alias
Genetic Interactions
Mapping
Mutants/Phenotypes
Protein Sequence Features
Hardwick KG and Pelham HR (1994) SED6 is identical to ERG6, and encodes a putative methyltransferase required for ergosterol synthesis. Yeast 10(2):265-9
     |ERD2
Cellular Location
Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
Leber R, et al. (1994) Characterization of lipid particles of the yeast, Saccharomyces cerevisiae. Yeast 10(11):1421-8

Alias
Mutants/Phenotypes
Strains/Constructs
Welihinda AA, et al. (1994) Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae. Biochim Biophys Acta 1193(1):107-17

Alias
Mutants/Phenotypes
Strains/Constructs
Graham TR, et al. (1993) Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway. EMBO J 12(3):869-77
     |PRC1
Mutants/Phenotypes
Strains/Constructs
Shah N and Klausner RD (1993) Brefeldin A reversibly inhibits secretion in Saccharomyces cerevisiae. J Biol Chem 268(8):5345-8

Mutants/Phenotypes
Strains/Constructs
Vogel JP, et al. (1993) Brefeldin A causes a defect in secretion in Saccharomyces cerevisiae. J Biol Chem 268(5):3040-3

Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
Heiderpriem RW, et al. (1992) A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae. J Steroid Biochem Mol Biol 43(7):741-3
     |ERG2
Reviews
Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
   |ACC1 |ACH1 |CDS1 |CHO1 |CHO2 |CKI1 |CTR1 |ERG1 |ERG10 |ERG11 |ERG12 |ERG13 |ERG2 |ERG20 |MORE
Cell Cycle Phase Involved
Function/Process
Mapping
Mutants/Phenotypes
Strains/Constructs
Gaber RF, et al. (1989) The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol Cell Biol 9(8):3447-56
     |SEC59
Mutants/Phenotypes
Strains/Constructs
Substrates/Ligands/Cofactors
Lorenz RT, et al. (1989) Structural discrimination in the sparking function of sterols in the yeast Saccharomyces cerevisiae. J Bacteriol 171(11):6169-73
     |ERG7 |HEM1
Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
Xu SH and Nes WD (1988) Biosynthesis of cholesterol in the yeast mutant erg6. Biochem Biophys Res Commun 155(1):509-17

Cell Cycle Phase Involved
Cellular Location
Mapping
Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
McCammon MT, et al. (1984) Sterol methylation in Saccharomyces cerevisiae. J Bacteriol 157(2):475-83
     |ERG3
Function/Process
Protein Processing/Modification/Regulation
Substrates/Ligands/Cofactors
Oehlschlager AC, et al. (1984) Azasterol inhibition of delta 24-sterol methyltransferase in Saccharomyces cerevisiae. Biochemistry 23(16):3582-9

Function/Process
Protein Physical Properties
Substrates/Ligands/Cofactors
Hata S, et al. (1982) Effect of detergents on sterol synthesis in a cell-free system of yeast. J Lipid Res 23(6):803-10
       |ERG1 |ERG9
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Substrates/Ligands/Cofactors
Techniques and Reagents
Kleinhans FW, et al. (1979) ESR determinations of membrane permeability in a yeast sterol mutant. Chem Phys Lipids 23(2):143-54
     |ERG2
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Substrates/Ligands/Cofactors
Lees ND, et al. (1979) ESR determination of membrane order parameter in yeast sterol mutants. Biochim Biophys Acta 553(3):469-75
     |ERG2
Function/Process
Pierce AM, et al. (1979) Azasterol inhibitors in yeast. Inhibition of the delta 24-sterol methyltransferase and the 24-methylene sterol delta 24(28)-reductase in sterol mutants of Saccharomyces cerevisiae. Can J Biochem 57(3):201-8
     |ERG2 |ERG5
Regulation of
Parks LW, et al. (1978) Sterols in yeast subcellular fractions. Lipids 13(10):730-5
     |ERG24 |ERG4
Function/Process
Fungal Related Genes/Proteins
Mutants/Phenotypes
Strains/Constructs
Pierce AM, et al. (1978) Metabolism of delta24-sterols by yeast mutants blocked in removal of the C-14 methyl group. Can J Biochem 56(8):794-800

Function/Process
Protein Processing/Modification/Regulation
Pierce HD Jr, et al. (1978) Azasterol inhibitors in yeast. Inhibition of the 24-methylene sterol delta24(28)-reductase and delta24-sterol methyltransferase of Saccharomyces cerevisiae by 23-azacholesterol. Biochim Biophys Acta 529(3):429-37
     |ERG4
Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
Bard M, et al. (1977) Sterol mutants of Saccharomyces cerevisiae: chromatographic analyses. Lipids 12(8):645-54
     |ERG2 |ERG3 |ERG5
Function/Process
Bailey RB, et al. (1976) Homoazasterol-mediated inhibition of yeast sterol biosynthesis. J Bacteriol 128(3):730-4

Cellular Location
Function/Process
Protein Processing/Modification/Regulation
Substrates/Ligands/Cofactors
Bailey RB and Parks LW (1975) Potassium translocation in yeast mitochondria and its relationship to ergostrol biosynthesis. J Bacteriol 122(2):606-9

Regulation of
Substrates/Ligands/Cofactors
Moore JT Jr and Gaylor JL (1970) Investigation of an S-adenosylmethionine: delta 24-sterol methyltransferase in ergosterol biosynthesis in yeast. Specificity of sterol substrates and inhibitors. J Biol Chem 245(18):4684-8

Alias
Function/Process
Protein Physical Properties
Substrates/Ligands/Cofactors
Moore JT and Gaylor JL (1969) Isolation and purification of an S-adenosylmethionine: delta 24-sterol methyltransferase from yeast. J Biol Chem 244(23):6334-40

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SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.