SUR4/YLR372W Single Page Format

This page provides an alternative format to the SGD Locus Summary Page. Note that additional information may be available on or linked from the standard format SGD Locus Summary page.

Contents
Names and Identifiers

GO Annotations

Pathways

Summary Paragraph

Mutant Phenotypes

Interactions

Homologs

Protein Info (physical properties, transcript info)

PDB Homologs (protein structure info)

Motifs

Genome-wide Expression
(and other large-scale analyses)

Locus History (misc. notes)

Sequence Retrieval and Analysis

Map and Displays

Localization

Community Annotation

Literature Guide

Sequence Coordinates

ChrXII: 867353 to 868390
CDS: 867353 - 868390

Click on map for expanded view

SGD ORF map GBrowse
SGD Locus Page

Names and Identifiers [TOP] [NEXT]
Standard Name Systematic Name Alias Feature Type SGDID

SUR4 YLR372W ELO3, SRE1, VBM1, APA1 ORF, Verified S000004364

See Nomenclature Note.

Description
Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis

GO Annotations [TOP] [NEXT]
Molecular Function
Annotation(s) Reference(s) Evidence Assigned By
fatty acid elongase activity Rossler H, et al. (2003) Functional differentiation and selective inactivation of multiple Saccharomyces cerevisiae genes involved in very-long-chain fatty acid synthesis. Mol Genet Genomics 269(2):290-8
       IMP : Inferred from Mutant Phenotype
Assigned on 2003-07-08 SGD
Biological Process
Annotation(s) Reference(s) Evidence Assigned By
fatty acid biosynthetic process Oh CS, et al. (1997) ELO2 and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J Biol Chem 272(28):17376-84
       IMP : Inferred from Mutant Phenotype
Assigned on 2001-01-18 SGD
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0275
Assigned on 2007-05-23 UniProtKB
fatty acid elongation Oh CS, et al. (1997) ELO2 and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J Biol Chem 272(28):17376-84
       IMP : Inferred from Mutant Phenotype
Assigned on 2002-09-23 SGD
lipid biosynthetic process GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0444
Assigned on 2007-05-23 UniProtKB
peptidyl-amino acid modification Huttenhower C and Troyanskaya OG (2009) Prediction of Gene Ontology annotations by integrating high-throughput datasets
   RCA : Reviewed Computational Analysis
Assigned on 2009-08-06 bioPIXIE_MEFIT
post-Golgi vesicle-mediated transport David D, et al. (1998) Involvement of long chain fatty acid elongation in the trafficking of secretory vesicles in yeast. J Cell Biol 143(5):1167-82
       IGI : Inferred from Genetic Interaction
Assigned on 2001-01-18 SGD
sphingolipid biosynthetic process Oh CS, et al. (1997) ELO2 and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J Biol Chem 272(28):17376-84
       IMP : Inferred from Mutant Phenotype
Assigned on 2002-09-23 SGD
Cellular Component
Annotation(s) Reference(s) Evidence Assigned By
endoplasmic reticulum Han G, et al. (2002) The Saccharomyces cerevisiae YBR159w gene encodes the 3-ketoreductase of the microsomal fatty acid elongase. J Biol Chem 277(38):35440-9
       IDA : Inferred from Direct Assay
Assigned on 2002-11-20 SGD
integral to membrane DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR002076
Assigned on 2007-05-23 UniProtKB
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0812
Assigned on 2007-05-23 UniProtKB
GOA curators and UniProt curators (2007) Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping.
     IEA : Inferred from Electronic Annotation with EBI:SL-9909
Assigned on 2009-10-01 UniProtKB
membrane GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0472
Assigned on 2009-06-29 UniProtKB

Pathways [TOP] [NEXT]
very long chain fatty acid biosynthesis

Summary Paragraph [TOP] [NEXT]
No summary paragraph available

Basic References [TOP]
BASIC INFORMATION REFERENCES forSUR4/YLR372W for SUR4
1) Oh CS, et al. (1997) ELO2 and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J Biol Chem 272(28):17376-84

2) Kobayashi SD and Nagiec MM (2003) Ceramide/long-chain base phosphate rheostat in Saccharomyces cerevisiae: regulation of ceramide synthesis by Elo3p and Cka2p. Eukaryot Cell 2(2):284-94

3) Desfarges L, et al. (1993) Yeast mutants affected in viability upon starvation have a modified phospholipid composition. Yeast 9(3):267-77

Mutant Phenotypes [TOP] [NEXT]
Phenotype page for SUR4/YLR372W

Interactions: genetic, physical, and other gene-gene links. [TOP] [NEXT]
Interaction page for SUR4/YLR372W

Homologs [TOP] [NEXT]

Comparison Resources

Physical Properties and Transcript Information: predicted from sequence [TOP] [NEXT]
Protein Sequence Calculations
from Predicted Full length Translation

N-term MNTTTST

C-term KVSSRKA

Length(aa) 345

MW(Da) 39,465

pI 10.13

Amino Acid Composition (full length)

GCG tools: PepPlot, Helical Wheel, PepStruct

Transcript Translation Calculations

Codon Bias 0.490

Codon Adaptation Index 0.372

Frequency of Optimal Codons 0.700

Hydropathicity of Protein 0.441

Aromaticity Score 0.168

10 20 30 40 50 | | | | | MNTTTSTVIAAVADQFQSLNSSSSCFLKVHVPSIENPFGIELWPIFSKVF EYFSGYPAEQFEFIHNKTFLANGYHAVSIIIVYYIIIFGGQAILRALNAS PLKFKLLFEIHNLFLTSISLVLWLLMLEQLVPMVYHNGLFWSICSKEAFA PKLVTLYYLNYLTKFVELIDTVFLVLRRKKLLFLHTYHHGATALLCYTQL IGRTSVEWVVILLNLGVHVIMYWYYFLSSCGIRVWWKQWVTRFQIIQFLI DLVFVYFATYTFYAHKYLDGILPNKGTCYGTQAAAAYGYLILTSYLLLFI SFYIQSYKKGGKKTVKKESEVSGSVASGSSTGVKTSNTKVSSRKA*

Protein Structures from PDB: proteins of known structure with sequence similarity to SUR4/YLR372W, based on Smith-Waterman analysis. [TOP] [NEXT]
No protein structure information available.

Genome-wide Expression and Other Large-Scale Analyses [TOP] [NEXT]

Functional Analysis
You can also search multiple datasets simultaneously using Expression Connection for expression studies or Function Junction for other large scale analyses.

Locus History (misc. notes) [TOP] [NEXT]
Nomenclature History
Standard Name Reference
SUR4 SGD (2007) Information without a citation in SGD

Nomenclature History Notes
Date Note
2004-11-19 APA1/YCL050C that encodes a diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I and SUR4/YLR372W that encodes a fatty acid elongase have both been referred to as APA1 in the literature.
Garcia-Arranz M, et al. (1994) Transcriptional control of yeast plasma membrane H(+)-ATPase by glucose. Cloning and characterization of a new gene involved in this regulation. J Biol Chem 269(27):18076-82

Plateau P, et al. (1989) Isolation, characterization, and inactivation of the APA1 gene encoding yeast diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase. J Bacteriol 171(12):6437-45

Standard Name	Reference
SUR4	SGD (2007) Information without a citation in SGD

Date	Note
2004-11-19	APA1/YCL050C that encodes a diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I and SUR4/YLR372W that encodes a fatty acid elongase have both been referred to as APA1 in the literature. *Garcia-Arranz M, et al.* (1994)** Transcriptional control of yeast plasma membrane H(+)-ATPase by glucose. Cloning and characterization of a new gene involved in this regulation. J Biol Chem 269(27):18076-82 *Plateau P, et al.* (1989)** Isolation, characterization, and inactivation of the APA1 gene encoding yeast diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase. J Bacteriol 171(12):6437-45

Sequence Retrieval [TOP] [NEXT]
Sequence Type Output Format

Genomic DNA GCG | FASTA | NoHeader

Genomic DNA with 1 kb up and downstream GCG | FASTA | NoHeader

DNA coding sequence
(without introns, without flanking regions) GCG | FASTA | NoHeader

Protein Translation of ORF GCG | FASTA | NoHeader

6-Frame Translation(with Restriction Map) GCG

Restriction Fragment Sizes GCG

Sequence Analysis Tools
Sequence from other databases

Sequence ID Source

YLR372W SGD Systematic Sequence

851087 NCBI: Gene ID

NP_013476.1 NCBI: RefSeq protein version ID

NP_013476.1 NCBI: RefSeq protein version ID

6323404 NCBI: NCBI protein GI

Sequence from other databases
Sequence ID	Source
YLR372W	SGD Systematic Sequence
851087	NCBI: Gene ID
NP_013476.1	NCBI: RefSeq protein version ID
NP_013476.1	NCBI: RefSeq protein version ID
6323404	NCBI: NCBI protein GI

Map and Displays [TOP] [NEXT]
Physical, Genetic Maps: Chromosomal Feature Map GBrowse Combined Physical and Genetic Map Genetic Distance vs. Physical Distance Ratios
Similarity Viewers: Synteny Viewer Genomic Stripe View SAGE Results Map

Localization [TOP] [NEXT]

Localization Resources

Community Annotation [TOP] [NEXT]
No community annotation available.

Literature Guide: papers categorized by topic. [TOP]
Topics Reference Other Genes Addressed
66 curated references; 0 references not yet curated
Genetic Interactions
Mutants/Phenotypes
Hodg CA, et al. (2010) Integral membrane proteins Brr6 and Apq12 link assembly of the nuclear pore complex to lipid homeostasis in the endoplasmic reticulum. J Cell Sci 123(Pt 1):141-151
       |ACC1 |APQ12 |ARE1 |ARE2 |ARV1 |BRR6 |DGA1 |ELO1 |ERG2 |ERG3 |ERG4 |ERG5 |ERG6 |FEN1 |MORE
Transcription
Momose Y, et al. (2010) Comparative analysis of transcriptional responses to the cryoprotectants, dimethyl sulfoxide and trehalose, which confer tolerance to freeze-thaw stress in Saccharomyces cerevisiae. Cryobiology
       |ACC1 |ARG1 |ARG3 |ARG4 |ARG5,6 |ARG7 |ARG8 |ATH1 |CPA1 |CPA2 |CRC1 |CYB2 |DAK1 |DAK2 |MORE
Function/Process
Mutants/Phenotypes
Brown JC and Lindquist S (2009) A heritable switch in carbon source utilization driven by an unusual yeast prion. Genes Dev 23(19):2320-32
       |HXT3 |MTH1 |PMA1 |RGT1 |SFB3 |SSA1 |SSA2 |STD1
Reviews
Ding J, et al. (2009) Tolerance and stress response to ethanol in the yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol 85(2):253-63
       |ADA2 |ASR1 |ATP1 |BEM2 |CHD1 |CTT1 |DDR2 |ERG6 |ERO1 |FEN1 |GCN5 |GIM4 |GIM5 |HFI1 |MORE
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
McCourt PC, et al. (2009) Stress-induced Ceramide-activated Protein Phosphatase Can Compensate for Loss of Amphiphysin-like Activity In Saccharomyces cerevisiae and Functions to Reinitiate Endocytosis. J Biol Chem 284(18):11930-41
       |CDC55 |PEP4 |PPH21 |PPH22 |RVS161 |RVS167 |SIT4 |STE3 |TPD3
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Morgan J, et al. (2009) Altering sphingolipid metabolism in Saccharomyces cerevisiae cells lacking the amphiphysin ortholog Rvs161 reinitiates sugar transporter endocytosis. Eukaryot Cell 8(5):779-89
       |CDC48 |DOA1 |DOA4 |GAL2 |HXT1 |HXT2 |HXT3 |HXT4 |MAL61 |RSP5 |RVS161 |SHP1 |SUC2 |UFD1
RNA Levels and Processing
Pedroso N, et al. (2009) Modulation of plasma membrane lipid profile and microdomains by H(2)O(2) in Saccharomyces cerevisiae. Free Radic Biol Med 46(2):289-98
       |ACC1 |ELO1 |ERG1 |ERG25 |ERG3 |ERG6 |ERG7 |FAS1 |FEN1 |GPT2 |LAC1 |LIP1 |OLE1 |RAD27
Cross-species Expression
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Quist TM, et al. (2009) HOS3, an ELO-Like Gene, Inhibits Effects of ABA and Implicates a S-1-P/Ceramide Control System for Abiotic Stress Responses in Arabidopsis thaliana. Mol Plant 2(1):138-151
     |FEN1
Genetic Interactions
Addinall SG, et al. (2008) A Genomewide Suppressor and Enhancer Analysis of cdc13-1 Reveals Varied Cellular Processes Influencing Telomere Capping in Saccharomyces cerevisiae. Genetics 180(4):2251-66
       |APQ12 |ARC18 |ARV1 |ASC1 |ASE1 |BFA1 |BIM1 |BMH1 |BRE2 |BUB1 |BUB2 |BUB3 |BUD27 |CCS1 |MORE
Reviews
Dickson RC (2008) Thematic Review Series: Sphingolipids. New insights into sphingolipid metabolism and function in budding yeast. J Lipid Res 49(5):909-21
       |AUR1 |CSG2 |CSH1 |DPL1 |FEN1 |IFA38 |IPT1 |ISC1 |LCB1 |LCB2 |LCB3 |LCB4 |LCB5 |LIP1 |MORE
Mutants/Phenotypes
Strains/Constructs
Grossmann G, et al. (2008) Plasma membrane microdomains regulate turnover of transport proteins in yeast. J Cell Biol 183(6):1075-88
       |CAN1 |CAX4 |COG1 |ELP6 |ERG2 |ERG24 |ERG5 |ERG6 |FUR4 |FYV6 |GOS1 |HNT3 |MDG1 |MNN10 |MORE
Genetic Interactions
Mutants/Phenotypes
Gustavsson M, et al. (2008) Functional genomics of monensin sensitivity in yeast: implications for post-Golgi traffic and vacuolar H(+)-ATPase function. Mol Genet Genomics 280(3):233-48
       |AKR1 |APL2 |ARC18 |ARF1 |BRE5 |BTS1 |CCZ1 |CDC50 |CHC1 |COG8 |EAF1 |EOS1 |ERG28 |FAB1 |MORE
Mutants/Phenotypes
Huang B, et al. (2008) A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA 14(10):2183-94
       |ADO1 |ARX1 |ATS1 |BUD19 |BUD21 |BUD28 |CFD1 |CHS3 |CHS7 |CIA1 |CKA2 |CST6 |CUP5 |DBP3 |MORE
Mutants/Phenotypes
Loukin S, et al. (2008) A genome-wide survey suggests an osmoprotective role for vacuolar Ca2+ release in cell wall-compromised yeast. FASEB J 22(7):2405-15
       |ALG5 |ALG6 |ALG8 |BST1 |CSF1 |DIE2 |GAS1 |GDA1 |GUP1 |HHY1 |HUR1 |KRE1 |KRE6 |LAS21 |MORE
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
McCue PP and Phang JM (2008) Identification of Human Intracellular Targets of the Medicinal Herb St. John's Wort by Chemical-Genetic Profiling in Yeast. J Agric Food Chem 56(22):11011-11017
       |ABD1 |ACS1 |ADP1 |AHC1 |APN2 |ASR1 |ATP5 |BCK2 |BFR1 |CBP6 |CHK1 |CIN8 |CRR1 |CTF19 |MORE
Function/Process
Genetic Interactions
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Ponnusamy S, et al. (2008) Regulation of Telomere Length by Fatty Acid Elongase 3 in Yeast: INVOLVEMENT OF INOSITOL PHOSPHATE METABOLISM AND Ku70/80 FUNCTION. J Biol Chem 283(41):27514-24
       |ARG82 |ELO1 |EST1 |FEN1 |IPK1 |ISC1 |KCS1 |LAG1 |RAD50 |TEL1 |YKU70 |YKU80
Alias
Fungal Related Genes/Proteins
Sakuradani E, et al. (2008) Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S-4. Appl Microbiol Biotechnol 81(3):497-503
       |ELO1 |FEN1
Function/Process
Soong TT, et al. (2008) Physical protein-protein interactions predicted from microarrays. Bioinformatics 24(22):2608-14
       |CTK3 |ERV25 |FEN1 |GCS1 |MEX67 |NPL3 |POB3 |PUB1 |SEC27 |VAC8 |YPT1
Mutants/Phenotypes
Strains/Constructs
Tang F, et al. (2008) A life-span extending form of autophagy employs the vacuole-vacuole fusion machinery. Autophagy 4(7):874-86
       |ATG1 |ATG10 |ATG11 |ATG15 |ATG17 |ATG22 |ATG7 |ATG8 |AVT3 |AVT4 |ERG2 |ERG28 |ERG5 |ERG6 |MORE
Computational analysis
RNA Levels and Processing
Alvarez-Vasquez F, et al. (2007) Coordination of the dynamics of yeast sphingolipid metabolism during the diauxic shift. Theor Biol Med Model 4:42
       |AUR1 |DPP1 |ELO1 |FAS1 |FAS2 |FEN1 |GPT2 |IPT1 |LAC1 |LAG1 |LCB1 |LCB2 |LCB4 |LCB5 |MORE
Reviews
Cowart LA and Obeid LM (2007) Yeast sphingolipids: recent developments in understanding biosynthesis, regulation, and function. Biochim Biophys Acta 1771(3):421-31
       |AUR1 |CSG2 |CSH1 |DPL1 |FEN1 |ISC1 |LAC1 |LAG1 |LCB1 |LCB2 |LCB3 |LCB4 |LCB5 |SUR1 |MORE
Function/Process
Mutants/Phenotypes
Strains/Constructs
Haass FA, et al. (2007) Identification of yeast proteins necessary for cell-surface function of a potassium channel. Proc Natl Acad Sci U S A 104(46):18079-18084
       |BST1 |CSG2 |EMP24 |ERV14 |ERV25 |TED1
Alias
Cross-species Expression
Fungal Related Genes/Proteins
Mutants/Phenotypes
Strains/Constructs
Prasitchoke P, et al. (2007) Functional analysis of very long-chain fatty acid elongase gene, HpELO2, in the methylotrophic yeast Hansenula polymorpha. Appl Microbiol Biotechnol 76(2):417-27
       |FEN1
Fungal Related Genes/Proteins
Prasitchoke P, et al. (2007) Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha. Gene 391(1-2):16-25
         |ELO1 |FEN1
Cross-species Expression
Genetic Interactions
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Qin YM, et al. (2007) Genetic and biochemical studies in yeast reveal that the cotton fibre-specific GhCER6 gene functions in fatty acid elongation. J Exp Bot 58(3):473-81
       |FEN1
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Shah Alam Bhuiyan M, et al. (2007) Synthetically lethal interactions involving loss of the yeast ERG24: the sterol C-14 reductase gene. Lipids 42(1):69-76
         |ERG2 |ERG24 |ERG28 |ERG3 |ERG6
Reviews
Tehlivets O, et al. (2007) Fatty acid synthesis and elongation in yeast. Biochim Biophys Acta 1771(3):255-70
       |ACC1 |ACP1 |BPL1 |CEM1 |ELO1 |ETR1 |FAS1 |FAS2 |FEN1 |HFA1 |HTD2 |IFA38 |MCT1 |OAR1 |MORE
Reviews
Toulmay A and Schneiter R (2007) Lipid-dependent surface transport of the proton pumping ATPase: A model to study plasma membrane biogenesis in yeast. Biochimie 89(2):249-54
       |AST1 |AUR1 |BUL1 |BUL2 |PMA1 |RSP5 |SFB3 |SOP4
Reviews
Dickson RC, et al. (2006) Functions and metabolism of sphingolipids in Saccharomyces cerevisiae. Prog Lipid Res 45(6):447-65
       |ARV1 |AUR1 |CSH1 |DPL1 |FEN1 |IPT1 |LCB1 |LCB2 |LCB3 |LCB4 |LCB5 |NCR1 |PKH1 |PKH2 |MORE
Cross-species Expression
Genetic Interactions
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Paul S, et al. (2006) Members of the Arabidopsis FAE1-like 3-ketoacyl-CoA synthase gene family substitute for the Elop proteins of Saccharomyces cerevisiae. J Biol Chem 281(14):9018-29
       |FEN1
Genetic Interactions
Tabuchi M, et al. (2006) The phosphatidylinositol 4,5-biphosphate and TORC2 binding proteins Slm1 and Slm2 function in sphingolipid regulation. Mol Cell Biol 26(15):5861-75
             |AUR1 |BCK1 |CMP2 |CNB1 |CSG2 |FEN1 |GAS1 |GIM3 |INO2 |INO4 |ISC1 |KRE1 |LDB16 |MSS4 |MORE
Mutants/Phenotypes
van Voorst F, et al. (2006) Genome-wide identification of genes required for growth of Saccharomyces cerevisiae under ethanol stress. Yeast 23(5):351-9
       |ASR1 |ATP1 |BEM2 |ERG6 |FAB1 |FEN1 |GCN5 |GIM4 |GIM5 |HMI1 |IMG1 |KAR3 |MSK1 |MSN2 |MORE
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Gaigg B, et al. (2005) Synthesis of sphingolipids with very long chain fatty acids but not ergosterol is required for routing of newly synthesized plasma membrane ATPase to the cell surface of yeast. J Biol Chem 280(23):22515-22
       |ACB1 |ACC1 |ARE1 |ARE2 |AYR1 |CSG2 |DPL1 |ELO1 |ERG24 |ERG3 |ERG4 |ERG5 |FEN1 |HEM1 |MORE
Genetic Interactions
Mutants/Phenotypes
Protein-protein Interactions
Strains/Constructs
Germann M, et al. (2005) Characterizing the sphingolipid signaling pathway that remediates defects associated with loss of the yeast amphiphysin-like orthologs, Rvs161p and Rvs167p. J Biol Chem 280(6):4270-8
       |ABP1 |ACF2 |ACT1 |AIM3 |FUS2 |GDH3 |LAS17 |RVS161 |RVS167 |SLA1
Alias
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Kvam E, et al. (2005) Targeting of Tsc13p to nucleus-vacuole junctions: a role for very-long-chain fatty acids in the biogenesis of microautophagic vesicles. Mol Biol Cell 16(9):3987-98
       |NVJ1 |TSC13 |VAC8
Function/Process
Mutants/Phenotypes
Strains/Constructs
Proszynski TJ, et al. (2005) A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast. Proc Natl Acad Sci U S A 102(50):17981-6
       |AYR1 |BUD27 |CCZ1 |CHS5 |ERG4 |ERG6 |FAB1 |GIM3 |KES1 |LCB1 |MCH5 |MON1 |OPI9 |PAC10 |MORE
Function/Process
Mutants/Phenotypes
Strains/Constructs
Zaremberg V, et al. (2005) Cytotoxicity of an anti-cancer lysophospholipid through selective modification of lipid raft composition. J Biol Chem 280(45):38047-58
       |ERG3 |FEN1 |LCB1 |PCT1 |PMA1 |SCS7
Mutants/Phenotypes
Strains/Constructs
Askree SH, et al. (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
           |ADE12 |ADO1 |AGP2 |APE3 |ARG2 |ARV1 |ASC1 |ATG11 |BEM4 |BRE2 |BRO1 |BUD16 |BUD30 |CAX4 |MORE
Genomic expression study
RNA Levels and Processing
Kim HJ, et al. (2004) A yeast DNA microarray for the evaluation of toxicity in environmental water containing burned ash. Environ Monit Assess 92(1-3):253-72
               |AAH1 |ATC1 |ATO2 |BOP2 |CAD1 |CCL1 |CCP1 |CDC42 |CRS5 |CSR2 |CTA1 |CTT1 |CUP1-1 |CUP1-2 |MORE
Cross-species Expression
Mietkiewska E, et al. (2004) Seed-specific heterologous expression of a nasturtium FAE gene in Arabidopsis results in a dramatic increase in the proportion of erucic acid. Plant Physiol 136(1):2665-75
           |FEN1
Alias
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Valachovic M, et al. (2004) A mutation in sphingolipid synthesis suppresses defects in yeast ergosterol metabolism. Lipids 39(8):747-52
     |ECM22 |ERG2 |FEN1 |UPC2
Function/Process
Mutants/Phenotypes
Strains/Constructs
Chung JH, et al. (2003) Sphingolipid requirement for generation of a functional v1 component of the vacuolar ATPase. J Biol Chem 278(31):28872-81
       |CUP5 |FEN1 |PPA1 |TFP1 |TFP3 |VMA10 |VMA2 |VMA5 |VMA6 |VMA7 |VPH1
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Kobayashi SD and Nagiec MM (2003) Ceramide/long-chain base phosphate rheostat in Saccharomyces cerevisiae: regulation of ceramide synthesis by Elo3p and Cka2p. Eukaryot Cell 2(2):284-94
       |CKA2 |LCB3
Function/Process
Mutants/Phenotypes
Strains/Constructs
Substrates/Ligands/Cofactors
Rossler H, et al. (2003) Functional differentiation and selective inactivation of multiple Saccharomyces cerevisiae genes involved in very-long-chain fatty acid synthesis. Mol Genet Genomics 269(2):290-8
       |ELO1 |FEN1 |IFA38
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Balguerie A, et al. (2002) Rvs161p and sphingolipids are required for actin repolarization following salt stress. Eukaryot Cell 1(6):1021-31
       |ACT1 |FEN1 |IPT1 |RVS161 |SLG1 |SUR1 |SUR2
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Eisenkolb M, et al. (2002) A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast. Mol Biol Cell 13(12):4414-28
         |ERG6 |GAS1 |PMA1
Alias
Reviews
Funato K, et al. (2002) Biosynthesis and trafficking of sphingolipids in the yeast Saccharomyces cerevisiae. Biochemistry 41(51):15105-14
     |ACB1 |AUR1 |CCC2 |CSG2 |DPL1 |ELO1 |FEN1 |FMP45 |IFA38 |IPT1 |ISC1 |LAC1 |LAG1 |LCB1 |MORE
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Gurunathan S, et al. (2002) t-SNARE phosphorylation regulates endocytosis in yeast. Mol Biol Cell 13(5):1594-607
         |SIT4 |SNC1 |STE2 |TLG1 |TLG2
Alias
Function/Process
Han G, et al. (2002) The Saccharomyces cerevisiae YBR159w gene encodes the 3-ketoreductase of the microsomal fatty acid elongase. J Biol Chem 277(38):35440-9
       |AYR1 |IFA38 |IRC24 |OAR1 |TMA29 |TSC13 |YDL114W
Genetic Interactions
Strains/Constructs
Huang D, et al. (2002) Dissection of a complex phenotype by functional genomics reveals roles for the yeast cyclin-dependent protein kinase Pho85 in stress adaptation and cell integrity. Mol Cell Biol 22(14):5076-88
           |ANP1 |BCK1 |BEM1 |BEM2 |BEM4 |BNI1 |BRE1 |CHO2 |CLA4 |CLG1 |EFR3 |ERD1 |FKS1 |FKS3 |MORE
Reviews
Obeid LM, et al. (2002) Yeast sphingolipids: metabolism and biology. Biochim Biophys Acta 1585(2-3):163-71
         |CSG2 |FEN1 |LCB4 |LCB5 |SUR1 |SUR2
Alias
Function/Process
Baudry K, et al. (2001) The effect of the erg26-1 mutation on the regulation of lipid metabolism in Saccharomyces cerevisiae. J Biol Chem 276(16):12702-11
       |ERG25 |ERG26 |ERG27 |FEN1
Alias
Genetic Interactions
Protein-protein Interactions
Kohlwein SD, et al. (2001) Tsc13p is required for fatty acid elongation and localizes to a novel structure at the nuclear-vacuolar interface in Saccharomyces cerevisiae. Mol Cell Biol 21(1):109-25
       |CSG2 |FEN1 |TSC13
Alias
Computational analysis
Radivoyevitch T (2001) Sphingoid base metabolism in yeast: mapping gene expression patterns into qualitative metabolite time course predictions. Comp Funct Genomics 2(5):289-94
             |AUR1 |DPL1 |FAS1 |FAS2 |FEN1 |LCB1 |LCB2 |LCB4 |LCB5
Techniques and Reagents
Chung N and Obeid LM (2000) Use of yeast as a model system for studies of sphingolipid metabolism and signaling. Methods Enzymol 311():319-31
     |FEN1 |LCB4 |LCB5 |SUR2
Alias
Genetic Interactions
Mutants/Phenotypes
Other Features
Strains/Constructs
Stock SD, et al. (2000) Syringomycin E inhibition of Saccharomyces cerevisiae: requirement for biosynthesis of sphingolipids with very-long-chain fatty acids and mannose- and phosphoinositol-containing head groups. Antimicrob Agents Chemother 44(5):1174-80
       |CSG2 |FEN1 |IPT1 |SEC14 |SUR1
Alias
Cell Cycle Phase Involved
Function/Process
Genetic Interactions
Mutants/Phenotypes
RNA Levels and Processing
Reviews
Strains/Constructs
Substrates/Ligands/Cofactors
Dickson RC and Lester RL (1999) Metabolism and selected functions of sphingolipids in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 1438(3):305-21
       |AUR1 |CSG2 |DPL1 |ERG2 |FAS2 |FEN1 |IPT1 |LCB1 |LCB2 |LCB3 |LCB4 |LCB5 |MSN2 |MSN4 |MORE
Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
Schneiter R, et al. (1999) Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane. J Cell Biol 146(4):741-54

Reviews
Daum G, et al. (1998) Biochemistry, cell biology and molecular biology of lipids of Saccharomyces cerevisiae. Yeast 14(16):1471-510
       |ACC1 |ACP1 |AUR1 |CDS1 |CEM1 |CHO1 |CHO2 |CKI1 |CPT1 |CRD1 |CSG2 |DPL1 |DPP1 |EPT1 |MORE
Alias
Function/Process
Mutants/Phenotypes
Strains/Constructs
David D, et al. (1998) Involvement of long chain fatty acid elongation in the trafficking of secretory vesicles in yeast. J Cell Biol 143(5):1167-82
       |FEN1 |SEC6 |SNC1 |SNC2
Alias
Function/Process
Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Oh CS, et al. (1997) ELO2 and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J Biol Chem 272(28):17376-84
       |ELO1 |FEN1
Function/Process
Mutants/Phenotypes
Strains/Constructs
Silve S, et al. (1996) The immunosuppressant SR 31747 blocks cell proliferation by inhibiting a steroid isomerase in Saccharomyces cerevisiae. Mol Cell Biol 16(6):2719-27
       |ERG2 |FEN1
DNA/RNA Sequence Features
Function/Process
Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Protein Sequence Features
Strains/Constructs
Revardel E, et al. (1995) Characterization of a new gene family developing pleiotropic phenotypes upon mutation in Saccharomyces cerevisiae. Biochim Biophys Acta 1263(3):261-5
     |FEN1 |RVS161 |RVS167
Alias
Function/Process
Mutants/Phenotypes
Protein Sequence Features
RNA Levels and Processing
Regulatory Role
Strains/Constructs
Garcia-Arranz M, et al. (1994) Transcriptional control of yeast plasma membrane H(+)-ATPase by glucose. Cloning and characterization of a new gene involved in this regulation. J Biol Chem 269(27):18076-82
     |GCR1 |HXT3 |PMA1 |RAP1 |SNF3
Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Desfarges L, et al. (1993) Yeast mutants affected in viability upon starvation have a modified phospholipid composition. Yeast 9(3):267-77
     |RVS161 |RVS167 |SUR1 |SUR2 |SUR3
Alias
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Naumovski L and Friedberg EC (1987) The RAD3 gene of Saccharomyces cerevisiae: isolation and characterization of a temperature-sensitive mutant in the essential function and of extragenic suppressors of this mutant. Mol Gen Genet 209(3):458-66
     |RAD3

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