NSP1/YJL041W Single Page Format

This page provides an alternative format to the SGD Locus Summary Page. Note that additional information may be available on or linked from the standard format SGD Locus Summary page.

Contents
Names and Identifiers

GO Annotations

Pathways

Summary Paragraph

Mutant Phenotypes

Interactions

Homologs

Protein Info (physical properties, transcript info)

PDB Homologs (protein structure info)

Motifs

Genome-wide Expression
(and other large-scale analyses)

Locus History (misc. notes)

Sequence Retrieval and Analysis

Map and Displays

Localization

Community Annotation

Literature Guide

Sequence Coordinates

ChrX: 365778 to 368367
intron: 365779 - 365896
CDS: 365778 - 365778, 365897 - 368367

Click on map for expanded view

SGD ORF map GBrowse
SGD Locus Page

Names and Identifiers [TOP] [NEXT]
Standard Name Systematic Name Alias Feature Type SGDID

NSP1 YJL041W ORF, Verified S000003577

See Nomenclature Note.

Description
Essential component of the nuclear pore complex, which mediates nuclear import and export, found in both the Nup82 and Nic96 complexes

GO Annotations [TOP] [NEXT]
Molecular Function
Annotation(s) Reference(s) Evidence Assigned By
structural constituent of nuclear pore DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR007758
Assigned on 2007-05-23 UniProtKB
structural molecule activity Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
Biological Process
Annotation(s) Reference(s) Evidence Assigned By
NLS-bearing substrate import into nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
RNA export from nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     IPI : Inferred from Physical Interaction
IGI : Inferred from Genetic Interaction
Assigned on 2001-01-18 SGD
cell cycle GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0131
Assigned on 2007-05-23 UniProtKB
establishment of protein localization Huttenhower C and Troyanskaya OG (2009) Prediction of Gene Ontology annotations by integrating high-throughput datasets
   RCA : Reviewed Computational Analysis
Assigned on 2009-08-06 bioPIXIE_MEFIT
mRNA export from nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
mRNA transport GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0509
Assigned on 2007-05-23 UniProtKB
mRNA-binding (hnRNP) protein import into nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
nuclear pore organization Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     IGI : Inferred from Genetic Interaction
IPI : Inferred from Physical Interaction
IMP : Inferred from Mutant Phenotype
Assigned on 2001-01-18 SGD
protein export from nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
protein import into nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     IMP : Inferred from Mutant Phenotype
Assigned on 2001-01-18 SGD
protein transport GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0653
Assigned on 2007-05-23 UniProtKB
rRNA export from nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
ribosomal large subunit export from nucleus Hurt E, et al. (1999) A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants. J Cell Biol 144(3):389-401
       IMP : Inferred from Mutant Phenotype
Assigned on 2001-01-18 SGD
ribosomal protein import into nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
snRNA export from nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
snRNP protein import into nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
tRNA export from nucleus Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     TAS : Traceable Author Statement
Assigned on 2001-01-18 SGD
transmembrane transport GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0811
Assigned on 2009-10-01 UniProtKB
transport GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0813
Assigned on 2007-05-23 UniProtKB
Cellular Component
Annotation(s) Reference(s) Evidence Assigned By
Nic96 complex Lutzmann M, et al. (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
       IDA : Inferred from Direct Assay
Assigned on 2009-10-14 SGD
Nup82 complex Lutzmann M, et al. (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
       IDA : Inferred from Direct Assay
Assigned on 2009-10-14 SGD
membrane GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0472
Assigned on 2008-06-16 UniProtKB
nuclear pore Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     IDA : Inferred from Direct Assay
Assigned on 2001-01-18 SGD
DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR007758
Assigned on 2007-05-23 UniProtKB
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0906
Assigned on 2007-05-23 UniProtKB
GOA curators and UniProt curators (2007) Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping.
     IEA : Inferred from Electronic Annotation with EBI:SL-0185
Assigned on 2009-05-06 UniProtKB
nucleus GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0539
Assigned on 2009-06-29 UniProtKB

Pathways [TOP] [NEXT]
No pathways available

Summary Paragraph [TOP] [NEXT]
SUMMARY PARAGRAPH for NSP1/YJL041W for NSP1
NSP1 encodes an essential nuclear pore protein (1, 2, 3, 4). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 4, 3, 5, 6). The structure of the vertebrate NPC has been studied extensively; recent reviews include 7, 8, 9, and 10. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (11, 12). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 4, 3, 13, 14, 15).
Numerous lines of evidence suggest that Nsp1p plays a key role in nucleocytoplasmic transport. Conditional mutations in NSP1 or depletion of Nsp1p cause defects in protein import into the nucleus (16), and Nsp1p depletion also reduces the number of NPCs (17). nsp1 mutants are also defective in export of ribosome subunits from the nucleus (18). Further, several other nucleoporin genes have been identified in yeast by screening for mutations that are synthetically lethal with conditional nsp1 mutations. (4)
Nsp1p is found in a subcomplex of the NPC that also contains Nup49p, Nup57p, and Nic96p (4, 19, 20). This complex has been detected at both the nuclear and cytoplasmic periphery of the NPC central channel (21). A second complex containing Nsp1p and Nup82p (22) is found only at the cytoplasmic periphery of the channel (21). Nsp1p also binds to the small GTPase Gsp1p in vitro (23), and can associate with the importin Pse1p (24).
Nsp1p is one of a group of nucleoporins that contain multiple repeats of the amino acids FXFG; deletion of the FXFG repeats has no effect on cell viability (2, 3, 4). Nsp1p shows sequence similarity to a nucleoporin, p62, found in NPCs from higher eukaryotes such as human (25) and rat (4).
Last Updated: 1999-06-25

Basic References [TOP]
BASIC INFORMATION REFERENCES forNSP1/YJL041W for NSP1
1) Hurt EC (1988) A novel nucleoskeletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae. EMBO J 7(13):4323-34

2) Nehrbass U, et al. (1990) NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain. Cell 61(6):979-89

3) Wente SR, et al. (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press

4) Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313

5) Pemberton LF, et al. (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9

6) Izaurralde E and Adam S (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64

7) Hinshaw JE (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20

8) Pante N and Aebi U (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99

9) Davis LI (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96

10) Pante N and Aebi U (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89

11) Rout MP and Blobel G (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83

12) Yang Q, et al. (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34

13) Doye V and Hurt E (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11

14) Doye V and Hurt EC (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41

15) Newmeyer DD (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407

16) Nehrbass U, et al. (1993) Analysis of nucleo-cytoplasmic transport in a thermosensitive mutant of nuclear pore protein NSP1. Eur J Cell Biol 62(1):1-12

17) Mutvei A, et al. (1992) NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation. Eur J Cell Biol 59(2):280-95

18) Hurt E, et al. (1999) A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants. J Cell Biol 144(3):389-401

19) Schlaich NL, et al. (1997) In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p. Mol Biol Cell 8(1):33-46

20) Grandi P, et al. (1993) Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J 12(8):3061-71

21) Fahrenkrog B, et al. (1998) Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol 143(3):577-88

22) Grandi P, et al. (1995) A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J Cell Biol 130(6):1263-73

23) Stochaj U, et al. (1998) The small GTPase Gsp1p binds to the repeat domain of the nucleoporin Nsp1p. Biochem J 330 ( Pt 1)():421-7

24) Seedorf M, et al. (1999) Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase. Mol Cell Biol 19(2):1547-57

25) Carmo-Fonseca M, et al. (1991) Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Eur J Cell Biol 55(1):17-30

26) Bailer SM, et al. (2001) The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport. Mol Cell Biol 21(23):7944-55

27) Lutzmann M, et al. (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51

Mutant Phenotypes [TOP] [NEXT]
Phenotype page for NSP1/YJL041W

Interactions: genetic, physical, and other gene-gene links. [TOP] [NEXT]
Interaction page for NSP1/YJL041W

Homologs [TOP] [NEXT]

Comparison Resources

Physical Properties and Transcript Information: predicted from sequence [TOP] [NEXT]
Protein Sequence Calculations
from Predicted Full length Translation

N-term MNFNTPQ

C-term QINSIKK

Length(aa) 823

MW(Da) 86,515

pI 6.13

Amino Acid Composition (full length)

GCG tools: PepPlot, Helical Wheel, PepStruct

Transcript Translation Calculations

Codon Bias 0.095

Codon Adaptation Index 0.165

Frequency of Optimal Codons 0.463

Hydropathicity of Protein -0.966

Aromaticity Score 0.083

10 20 30 40 50 | | | | | MNFNTPQQNKTPFSFGTANNNSNTTNQNSSTGAGAFGTGQSTFGFNNSAP NNTNNANSSITPAFGSNNTGNTAFGNSNPTSNVFGSNNSTTNTFGSNSAG TSLFGSSSAQQTKSNGTAGGNTFGSSSLFNNSTNSNTTKPAFGGLNFGGG NNTTPSSTGNANTSNNLFGATANANKPAFSFGATTNDDKKTEPDKPAFSF NSSVGNKTDAQAPTTGFSFGSQLGGNKTVNEAAKPSLSFGSGSAGANPAG ASQPEPTTNEPAKPALSFGTATSDNKTTNTTPSFSFGAKSDENKAGATSK PAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEK NNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKPDENKASATSKPAFSF GAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNET SKPAFSFGAKSDEKKDGDASKPAFSFGAKSDEKKDSDSSKPAFSFGTKSN EKKDSGSSKPAFSFGAKPDEKKNDEVSKPAFSFGAKANEKKESDESKSAF SFGSKPTGKEEGDGAKAAISFGAKPEEQKSSDTSKPAFTFGAQKDNEKKT EESSTGKSTADVKSSDSLKLNSKPVELKPVSLDNKTLDDLVTKWTNQLTE SASHFEQYTKKINSWDQVLVKGGEQISQLYSDAVMAEHSQNKIDQSLQYI ERQQDELENFLDNFETKTEALLSDVVSTSSGAAANNNDQKRQQAYKTAQT LDENLNSLSSNLSSLIVEINNVSNTFNKTTNIDINNEDENIQLIKILNSH FDALRSLDDNSTSLEKQINSIKK*

Protein Structures from PDB: proteins of known structure with sequence similarity to NSP1/YJL041W, based on Smith-Waterman analysis. [TOP] [NEXT]
PDB protein structure(s) homologous to NSP1 Homolog Source (per PDB) Protein Alignment: NSP1 vs. Homolog External Links
P-Value %Identical %Similar Alignment
1o6o ( Chain: E, F, D)
Importin beta aa1-442 bound to five fxfg repeats from yeast nsp1p. second crystal form
PDB_Info
PDB_Structure
Homo sapiens | Saccharomyces cerevisiae Chain E = 4.5e-34 100 0 View alignment SCOP
MMDB
CATH
Chain F = 4.5e-34 100 0 View alignment
Chain D = 4.5e-34 100 0 View alignment
1wxr ( Chain: A)
Crystal structure of heme binding protein, an autotransporter hemoglobine protease from pathogenic escherichia coli
PDB_Info
PDB_Structure
Escherichia coli 0.000539 21 34 View alignment SCOP
MMDB
CATH
3hqv ( Chain: A, C)
Collagen alpha-1(I) ch
PDB_Info
PDB_Structure
Unknown Chain A = 0.009803 22 28 View alignment SCOP
MMDB
CATH
Chain C = 0.009803 22 28 View alignment
3hr2 ( Chain: A, C)
Collagen alpha-1(I) ch
PDB_Info
PDB_Structure
Unknown Chain A = 0.009803 22 28 View alignment SCOP
MMDB
CATH
Chain C = 0.009803 22 28 View alignment

Genome-wide Expression and Other Large-Scale Analyses [TOP] [NEXT]

Functional Analysis
You can also search multiple datasets simultaneously using Expression Connection for expression studies or Function Junction for other large scale analyses.

Locus History (misc. notes) [TOP] [NEXT]
Nomenclature History
Standard Name Reference
NSP1 SGD (2007) Information without a citation in SGD

Nomenclature History Notes
Date Note
2003-12-09 Both YJL041W/NSP1 and YLR178C/TFS1 have been called NSP1
Mapping Notes
Date Note
1998-11-10 Edition 15: The name NSP1 has also been used to refer to TFS1/YLR178C [Tripp et al. [1989] Mol Microbiol 3:1319-1327]. Do not confuse TFS1/NSP1/YLR178C with NSP1/YJL041W, a nuclear pore protein on chromosome 10.
Cherry JM, et al. (1998) "Genetic and Physical Maps of Saccharomyces cerevisiae (Edition 15)". Pp. 414-420 in 1998 Yeast Genetics and Molecular Biology Meeting Program and Abstracts. Bethesda, MD: The Genetics Society of America

Standard Name	Reference
NSP1	SGD (2007) Information without a citation in SGD

Date	Note
2003-12-09	Both YJL041W/NSP1 and YLR178C/TFS1 have been called NSP1

Date	Note
1998-11-10	Edition 15: The name NSP1 has also been used to refer to TFS1/YLR178C [Tripp et al. [1989] Mol Microbiol 3:1319-1327]. Do not confuse TFS1/NSP1/YLR178C with NSP1/YJL041W, a nuclear pore protein on chromosome 10. *Cherry JM, et al.* (1998)** "Genetic and Physical Maps of Saccharomyces cerevisiae (Edition 15)". Pp. 414-420 in 1998 Yeast Genetics and Molecular Biology Meeting Program and Abstracts. Bethesda, MD: The Genetics Society of America

Sequence Retrieval [TOP] [NEXT]
Sequence Type Output Format

Genomic DNA GCG | FASTA | NoHeader

Genomic DNA with 1 kb up and downstream GCG | FASTA | NoHeader

DNA coding sequence
(without introns, without flanking regions) GCG | FASTA | NoHeader

Protein Translation of ORF GCG | FASTA | NoHeader

6-Frame Translation(with Restriction Map) GCG

Restriction Fragment Sizes GCG

Sequence Analysis Tools
Sequence from other databases

Sequence ID Source

YJL041W SGD Systematic Sequence

853409 NCBI: Gene ID

NP_012494.1 NCBI: RefSeq protein version ID

NP_012494.1 NCBI: RefSeq protein version ID

6322420 NCBI: NCBI protein GI

Sequence from other databases
Sequence ID	Source
YJL041W	SGD Systematic Sequence
853409	NCBI: Gene ID
NP_012494.1	NCBI: RefSeq protein version ID
NP_012494.1	NCBI: RefSeq protein version ID
6322420	NCBI: NCBI protein GI

Map and Displays [TOP] [NEXT]
Physical, Genetic Maps: Chromosomal Feature Map GBrowse Combined Physical and Genetic Map Genetic Distance vs. Physical Distance Ratios
Similarity Viewers: Synteny Viewer Genomic Stripe View SAGE Results Map

Localization [TOP] [NEXT]

Localization Resources

Community Annotation [TOP] [NEXT]
No community annotation available.

Literature Guide: papers categorized by topic. [TOP]
Topics Reference Other Genes Addressed
89 curated references; 0 references not yet curated
Protein-protein Interactions
Luo Y, et al. (2010) Resolving the Composition of Protein Complexes Using a MALDI LTQ Orbitrap. J Am Soc Mass Spectrom 21(1):34-46
       |APL1 |APL3 |APM4 |APS2 |ASM4 |BMH2 |EDE1 |FKS1 |NIC96 |NUP120 |NUP133 |NUP145 |NUP159 |NUP170 |MORE
Protein-protein Interactions
Strains/Constructs
Alberti S, et al. (2009) A systematic survey identifies prions and illuminates sequence features of prionogenic proteins. Cell 137(1):146-58
       |AIM3 |AKL1 |ASM4 |AZF1 |CAF40 |CBK1 |CCR4 |CLA4 |CYC8 |DDR48 |DEF1 |ENT1 |ENT2 |EPL1 |MORE
Mutants/Phenotypes
Protein Sequence Features
Protein-protein Interactions
Strains/Constructs
Beliakova-Bethell N, et al. (2009) Ty3 nuclear entry is initiated by viruslike particle docking on GLFG nucleoporins. J Virol 83(22):11914-25
       |NUP100 |NUP116 |NUP120 |NUP133 |NUP145 |NUP159 |NUP42 |NUP49 |NUP57 |YGR109W-A |YGR109W-B |YIL080W |YIL082W |YIL082W-A
Protein Sequence Features
Protein-protein Interactions
Hobeika M, et al. (2009) Structural Requirements for the Ubiquitin-associated Domain of the mRNA Export Factor Mex67 to Bind Its Specific Targets, the Transcription Elongation THO Complex Component Hpr1 and Nucleoporin FXFG Repeats. J Biol Chem 284(26):17575-83
       |HPR1 |MEX67
Other Features
Protein-protein Interactions
Wang Y, et al. (2009) Abnormal proteins can form aggresome in yeast: aggresome-targeting signals and components of the machinery. FASEB J 23(2):451-63
       |ADH1 |ATP2 |BMH1 |CDC48 |DSK2 |ENO2 |FBA1 |HSP42 |HSP60 |HSP82 |ILV5 |IPP1 |MDR1 |NOG2 |MORE
Fungal Related Genes/Proteins
Dunn B and Sherlock G (2008) Reconstruction of the genome origins and evolution of the hybrid lager yeast Saccharomyces pastorianus. Genome Res 18(10):1610-23
       |APS3 |BET1 |BET4 |DBP2 |IMD4 |MOB1 |PRE3 |REC107 |TEF4 |YJR079W
Cellular Location
Strains/Constructs
Harper NC, et al. (2008) Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae. Curr Genet 53(2):95-105
       |BFA1 |BUB2 |DBF2 |LTE1 |NIC96 |NUD1 |NUP1 |NUP170 |NUP60 |SWI5
Genetic Interactions
Mutants/Phenotypes
Protein-protein Interactions
Strains/Constructs
Hung NJ, et al. (2008) Arx1 Is a Nuclear Export Receptor for the 60S Ribosomal Subunit in Yeast. Mol Biol Cell 19(2):735-44
       |ARX1 |CRM1 |GLE2 |MEX67 |MTR2 |NIC96 |NMD3 |NUP100 |NUP116 |NUP120 |NUP133 |NUP42 |NUP57 |NUP82 |MORE
Protein Sequence Features
Protein-protein Interactions
Strains/Constructs
Substrates/Ligands/Cofactors
Patel SS and Rexach MF (2008) Discovering novel interactions at the nuclear pore complex using bead halo: a rapid method for detecting molecular interactions of high and low affinity at equilibrium. Mol Cell Proteomics 7(1):121-31
       |ASM4 |GLE1 |KAP95 |NDC1 |NIC96 |NUP1 |NUP100 |NUP116 |NUP53 |NUP60 |NUP84 |POM34 |SEH1 |SRM1 |MORE
Protein Processing/Modification/Regulation
Shen Y, et al. (2008) Mass spectrometry analysis of proteome-wide proteolytic post-translational degradation of proteins. Anal Chem 80(15):5819-28
       |ACT1 |BFR1 |COF1 |FBA1 |HSP10 |KAR2 |NUP2 |OM45 |PGK1 |PRE2 |PRE3 |PUP1 |RPL3 |SRV2 |MORE
Protein-protein Interactions
Tarassov K, et al. (2008) An in vivo map of the yeast protein interactome. Science 320(5882):1465-70
       |ACT1 |ARC40 |ARP2 |ATG27 |BEM1 |CDC11 |CHC1 |DHH1 |FMP42 |GYL1 |KEL1 |KEL2 |LAS17 |LSM4 |MORE
Cellular Location
Computational analysis
Protein Physical Properties
Protein-protein Interactions
Protein/Nucleic Acid Structure
Alber F, et al. (2007) Determining the architectures of macromolecular assemblies. Nature 450(7170):683-94
           |ASM4 |GLE1 |GLE2 |NDC1 |NIC96 |NUP1 |NUP100 |NUP116 |NUP120 |NUP133 |NUP145 |NUP157 |NUP159 |NUP170 |MORE
Cellular Location
Evolution
Protein/Nucleic Acid Structure
Alber F, et al. (2007) The molecular architecture of the nuclear pore complex. Nature 450(7170):695-701
           |ASM4 |GLE1 |GLE2 |NDC1 |NIC96 |NUP1 |NUP100 |NUP116 |NUP120 |NUP133 |NUP145 |NUP157 |NUP159 |NUP170 |MORE
Evolution
Protein Sequence Features
Protein/Nucleic Acid Structure
Denning DP and Rexach MF (2007) Rapid evolution exposes the boundaries of domain structure and function in natively unfolded FG nucleoporins. Mol Cell Proteomics 6(2):272-82
         |ASM4 |NUP1 |NUP100 |NUP116 |NUP159 |NUP2 |NUP42 |NUP49 |NUP53 |NUP57 |NUP60
Reviews
Kohler A and Hurt E (2007) Exporting RNA from the nucleus to the cytoplasm. Nat Rev Mol Cell Biol 8(10):761-73
       |ARC1 |ARX1 |CDC31 |CEX1 |CRM1 |DBP5 |GLE1 |GLE2 |HTZ1 |LOS1 |MEX67 |MLP1 |MLP2 |MTR2 |MORE
Protein-protein Interactions
Techniques and Reagents
Oeffinger M, et al. (2007) Comprehensive analysis of diverse ribonucleoprotein complexes. Nat Methods 4(11):951-6
       |ACB1 |ACT1 |ADE3 |AIM34 |ASH1 |ASM4 |BEM2 |BRR2 |BRX1 |BUD8 |CBC2 |CBF5 |CDC3 |CDC31 |MORE
Cellular Location
Function/Process
Protein Sequence Features
Protein-protein Interactions
Patel SS, et al. (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
         |GLE1 |GLE2 |KAP95 |NDC1 |NIC96 |NUP1 |NUP100 |NUP116 |NUP120 |NUP157 |NUP159 |NUP170 |NUP192 |NUP2 |MORE
RNA Levels and Processing
Pleiss JA, et al. (2007) Rapid, transcript-specific changes in splicing in response to environmental stress. Mol Cell 27(6):928-37
           |ARP9 |DCN1 |GCN2 |HIS4 |KEI1 |KEM1 |NAM7 |NOG2 |PRP8 |RAI1 |RPL14A |RPL14B |RPL17B |RPL18B |MORE
Protein Processing/Modification/Regulation
Regulation of
Smolka MB, et al. (2007) Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases. Proc Natl Acad Sci U S A 104(25):10364-9
       |CEP3 |CPR5 |CTF4 |DEF1 |DUN1 |EXO1 |HPC2 |ITC1 |MEC1 |MLP1 |NET1 |NPL3 |NUP1 |NUP2 |MORE
Genetic Interactions
Mutants/Phenotypes
Protein-protein Interactions
Stelter P, et al. (2007) Molecular basis for the functional interaction of dynein light chain with the nuclear-pore complex. Nat Cell Biol 9(7):788-96
       |DYN1 |DYN2 |NUP133 |NUP159 |NUP82 |PEX14
Reviews
Taddei A (2007) Active genes at the nuclear pore complex. Curr Opin Cell Biol 19(3):305-310
       |ASM4 |CDC31 |DBP5 |GAL1 |GFD1 |GLE1 |GLE2 |HSP104 |HTZ1 |INO1 |KAP120 |KAP122 |KAP123 |KAP95 |MORE
Function/Process
Genetic Interactions
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Terry LJ and Wente SR (2007) Nuclear mRNA export requires specific FG nucleoporins for translocation through the nuclear pore complex. J Cell Biol 178(7):1121-32
       |KAP104 |MEX67 |NUP1 |NUP100 |NUP116 |NUP145 |NUP2 |NUP49 |NUP57 |NUP60 |PSE1
Evolution
Fungal Related Genes/Proteins
De Hertogh B, et al. (2006) Emergence of species-specific transporters during evolution of the hemiascomycete phylum. Genetics 172(2):771-81
       |AAC1 |AAC3 |ACS2 |ADP1 |ADY2 |AGC1 |AGP1 |AGP2 |AGP3 |ALP1 |ALR1 |ALR2 |ANT1 |AQR1 |MORE
Computational analysis
Non-Fungal Related Genes/Proteins
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Devos D, et al. (2006) Simple fold composition and modular architecture of the nuclear pore complex. Proc Natl Acad Sci U S A 103(7):2172-7
       |ASM4 |NDC1 |NIC96 |NUP1 |NUP100 |NUP116 |NUP120 |NUP133 |NUP145 |NUP157 |NUP159 |NUP170 |NUP188 |NUP192 |MORE
Function/Process
Genetic Interactions
Mutants/Phenotypes
Protein Physical Properties
Protein Sequence Features
Protein-protein Interactions
Strains/Constructs
Frey S, et al. (2006) FG-rich repeats of nuclear pore proteins form a three-dimensional meshwork with hydrogel-like properties. Science 314(5800):815-7
       |NUP116 |NUP145 |NUP49 |NUP57
Cellular Location
Miao M, et al. (2006) The integral membrane protein pom34p functionally links nucleoporin subcomplexes. Genetics 172(3):1441-57
         |ASM4 |GLE2 |NUP100 |NUP116 |NUP120 |NUP159 |NUP170 |NUP188 |NUP2 |NUP49 |NUP57 |NUP82 |POM152 |POM34 |MORE
Protein Physical Properties
Protein-protein Interactions
Strains/Constructs
Lutzmann M, et al. (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
       |GLE2 |KAP123 |MEX67 |MLP1 |NIC96 |NUP116 |NUP120 |NUP133 |NUP145 |NUP157 |NUP159 |NUP170 |NUP49 |NUP57 |MORE
Non-Fungal Related Genes/Proteins
Protein Processing/Modification/Regulation
Mason DA, et al. (2005) Increased nuclear envelope permeability and Pep4p-dependent degradation of nucleoporins during hydrogen peroxide-induced cell death. FEMS Yeast Res 5(12):1237-51
       |MCA1 |NUP1 |NUP100 |NUP116 |NUP159 |NUP53 |NUP84 |NUP85 |PAI3 |PEP4 |POM152 |PRB1 |SEH1
Function/Process
Mutants/Phenotypes
Protein Physical Properties
Protein-protein Interactions
Kiseleva E, et al. (2004) Yeast nuclear pore complexes have a cytoplasmic ring and internal filaments. J Struct Biol 145(3):272-88
               |NUP116 |NUP159 |NUP49 |NUP57
Protein Physical Properties
Protein Sequence Features
Denning DP, et al. (2003) Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded. Proc Natl Acad Sci U S A 100(5):2450-5
         |ASM4 |CDC31 |GLE1 |GLE2 |NDC1 |NIC96 |NUP1 |NUP100 |NUP116 |NUP120 |NUP133 |NUP145 |NUP157 |NUP159 |MORE
Cellular Location
Protein-protein Interactions
Bailer SM, et al. (2001) The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport. Mol Cell Biol 21(23):7944-55
       |NIC96 |NUP49 |NUP57 |NUP82
Function/Process
Mutants/Phenotypes
Strains/Constructs
Gleizes PE, et al. (2001) Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex. J Cell Biol 155(6):923-36
       |DBP5 |GLE1 |GSP1 |NUP159 |NUP82 |NUP84
Cellular Location
Function/Process
Grosshans H, et al. (2001) Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export. J Cell Biol 153(4):745-62
       |CRM1 |DIS3 |KAP123 |MEX67 |MTR10 |NUP159 |NUP85 |PSE1 |RNA1 |RRP4 |SCR1 |SEC65 |SRM1 |SRP1 |MORE
Reviews
Vasu SK and Forbes DJ (2001) Nuclear pores and nuclear assembly. Curr Opin Cell Biol 13(3):363-75
       |ASM4 |CDC31 |GLE1 |GLE2 |MLP1 |MLP2 |NDC1 |NIC96 |NUP1 |NUP100 |NUP116 |NUP120 |NUP133 |NUP145 |MORE
Cellular Location
Genetic Interactions
Mutants/Phenotypes
Protein-protein Interactions
Strains/Constructs
Bailer SM, et al. (2000) Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex. J Biol Chem 275(31):23540-8
       |GLE2 |NUP116 |NUP159 |NUP82
Protein Sequence Features
Protein-protein Interactions
Protein/Nucleic Acid Structure
Bayliss R, et al. (2000) Crystallization and initial X-ray diffraction characterization of complexes of FxFG nucleoporin repeats with nuclear transport factors. J Struct Biol 131(3):240-7
                 |NTF2
Protein/Nucleic Acid Structure
Bayliss R, et al. (2000) Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking. Cell 102(1):99-108

DNA/RNA Sequence Features
Davis CA, et al. (2000) Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast. Nucleic Acids Res 28(8):1700-6
       |AIM11 |AMA1 |AML1 |APE2 |APS3 |ARP9 |ASC1 |BET1 |BIG1 |BOS1 |CDC21 |COF1 |DCN1 |DID4 |MORE
Cellular Location
Function/Process
Strains/Constructs
Fahrenkrog B, et al. (2000) Comparative spatial localization of protein-A-tagged and authentic yeast nuclear pore complex proteins by immunogold electron microscopy. J Struct Biol 129(2-3):295-305
       |NIC96
Cellular Location
Protein-protein Interactions
Ho AK, et al. (2000) Assembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p. Mol Cell Biol 20(15):5736-48
       |GLE1 |NUP116 |NUP159 |NUP82
Function/Process
Protein-protein Interactions
Techniques and Reagents
Jones AL, et al. (2000) SAC3 may link nuclear protein export to cell cycle progression. Proc Natl Acad Sci U S A 97(7):3224-9
       |CRM1 |SAC3 |SDS23 |SDS24 |YRB2
Cellular Location
Protein Physical Properties
Strains/Constructs
Techniques and Reagents
Rout MP, et al. (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
       |AIM4 |ASM4 |BMS1 |BUD20 |CTR9 |GLE1 |GLE2 |HAS1 |KAP120 |KAP122 |KAP123 |KAP95 |KRR1 |MEX67 |MORE
Reviews
Ryan KJ and Wente SR (2000) The nuclear pore complex: a protein machine bridging the nucleus and cytoplasm. Curr Opin Cell Biol 12(3):361-71
       |CRM1 |GLE1 |KAP104 |KAP123 |KAP95 |LOS1 |MSN5 |MTR10 |NIC96 |NMD5 |NUP116 |NUP159 |NUP170 |NUP82 |MORE
Function/Process
Mutants/Phenotypes
Strains/Constructs
Substrates/Ligands/Cofactors
Stage-Zimmermann T, et al. (2000) Factors affecting nuclear export of the 60S ribosomal subunit in vivo. Mol Biol Cell 11(11):3777-89
       |CRM1 |CSE1 |DBP5 |FAL1 |GLE1 |GLE2 |KAP104 |KAP120 |KAP123 |KAP95 |MEX67 |MSN5 |MTR10 |MTR2 |MORE
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Strasser K, et al. (2000) Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export. J Cell Biol 150(4):695-706
       |GLE2 |MEX67 |MTR2 |NUP116 |NUP159 |NUP42
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Tan Q, et al. (2000) Multiple mechanisms of suppression circumvent transcription defects in an RNA polymerase mutant. Mol Cell Biol 20(21):8124-33
         |GAL1 |HSP26 |INO1 |MSN2 |PHO5 |RPB4 |RPB7 |SRO9 |SSA1 |SSA3
Function/Process
Protein-protein Interactions
Bayliss R, et al. (1999) Interaction between NTF2 and xFxFG-containing nucleoporins is required to mediate nuclear import of RanGDP. J Mol Biol 293(3):579-93
       |NTF2
Genetic Interactions
Mutants/Phenotypes
Hurt E, et al. (1999) A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants. J Cell Biol 144(3):389-401
       |GSP1 |LOS1 |NUP49 |RNA1 |RPL25 |SRM1
RNA Levels and Processing
Regulation of
Jelinsky SA and Samson LD (1999) Global response of Saccharomyces cerevisiae to an alkylating agent. Proc Natl Acad Sci U S A 96(4):1486-91
             |AAD14 |AAD16 |AAD4 |AAD6 |ABF1 |ADP1 |AGX1 |AMD2 |ARG1 |ARG3 |ARG4 |ARG5,6 |ARG7 |ARO1 |MORE
Protein-protein Interactions
Seedorf M, et al. (1999) Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase. Mol Cell Biol 19(2):1547-57
       |CRM1 |GLE2 |GSP1 |KAP95 |NUP1 |NUP116 |NUP145 |NUP159 |NUP82 |POM152 |PSE1 |RNA1 |SRM1 |SRP1 |MORE
Reviews
Strasser K and Hurt E (1999) Nuclear RNA export in yeast. FEBS Lett 452(1-2):77-81
     |GLE1 |MEX67 |MTR10 |MTR2 |NPL3 |NUP120 |NUP145 |NUP159 |NUP82 |NUP85
Reviews
Wolin SL and Matera AG (1999) The trials and travels of tRNA. Genes Dev 13(1):1-10
       |ARC1 |GCD11 |GSP1 |LHP1 |LOS1 |PUS1 |TRM1
Protein-protein Interactions
Belgareh N, et al. (1998) Functional characterization of a Nup159p-containing nuclear pore subcomplex. Mol Biol Cell 9(12):3475-92
       |NIC96 |NUP159 |NUP82
Cellular Location
Function/Process
Protein Processing/Modification/Regulation
Techniques and Reagents
Bucci M and Wente SR (1998) A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly. Mol Biol Cell 9(9):2439-61
       |GLE2 |NIC96 |NUP116 |NUP120 |NUP145 |NUP159 |NUP49 |NUP57 |NUP82 |POM152
Cellular Location
Function/Process
Strains/Constructs
Techniques and Reagents
Fahrenkrog B, et al. (1998) Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol 143(3):577-88
       |NIC96 |NUP49 |NUP57 |NUP82
Protein-protein Interactions
Hellmuth K, et al. (1998) Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA. Mol Cell Biol 18(11):6374-86
       |GCD11 |GSP1 |GSP2 |LOS1 |MEX67 |NUP2 |YRB1
Function/Process
Protein Sequence Features
Protein-protein Interactions
Stochaj U, et al. (1998) The small GTPase Gsp1p binds to the repeat domain of the nucleoporin Nsp1p. Biochem J 330 ( Pt 1)():421-7
       |GSP1
Cellular Location
Function/Process
Techniques and Reagents
Vodicka MA, et al. (1998) HIV-1 Vpr interacts with the nuclear transport pathway to promote macrophage infection. Genes Dev 12(2):175-85
       |KAP95 |SRP1
Non-Fungal Related Genes/Proteins
Techniques and Reagents
Yang Q, et al. (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
       |GLE1 |NIC96 |NUP1 |NUP100 |NUP116 |NUP120 |NUP133 |NUP145 |NUP157 |NUP159 |NUP170 |NUP188 |NUP2 |NUP42 |MORE
Reviews
Fabre E and Hurt E (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
     |ACC1 |ASM4 |CBC2 |GLE1 |GLE2 |GSP1 |HRP1 |KAP104 |KAP123 |KAP95 |LOS1 |MEX67 |MTR2 |MTR3 |MORE
Non-Fungal Related Genes/Proteins
Protein-protein Interactions
Grandi P, et al. (1997) Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly. Mol Biol Cell 8(10):2017-38
       |NIC96 |NUP192
Cellular Location
Protein Sequence Features
Protein-protein Interactions
Strains/Constructs
Techniques and Reagents
Schlaich NL, et al. (1997) In vitro reconstitution of a heterotrimeric nucleoporin complex consisting of recombinant Nsp1p, Nup49p, and Nup57p. Mol Biol Cell 8(1):33-46
     |NIC96 |NUP49 |NUP57
Reviews
Wente SR, et al. (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
   |GLE1 |GLE2 |GSP1 |GSP2 |KAP95 |MTR2 |NIC96 |NPL3 |NTF2 |NUP1 |NUP100 |NUP116 |NUP120 |NUP133 |MORE
Cell Cycle Phase Involved
Cellular Location
Winey M, et al. (1997) Nuclear pore complex number and distribution throughout the Saccharomyces cerevisiae cell cycle by three-dimensional reconstruction from electron micrographs of nuclear envelopes. Mol Biol Cell 8(11):2119-32
       |NIC96 |NUP1 |NUP100 |NUP116 |NUP120 |NUP133 |NUP145 |NUP157 |NUP159 |NUP170 |NUP188 |NUP2 |NUP42 |NUP49 |MORE
Function/Process
Other Features
Protein Sequence Features
Protein-protein Interactions
Barth W and Stochaj U (1996) The yeast nucleoporin Nsp1 binds nuclear localization sequences in vitro. Biochem Cell Biol 74(3):363-72

Cross-species Expression
Clarkson WD, et al. (1996) Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p. J Mol Biol 263(4):517-24
                 |NTF2
Reviews
Pante N and Aebi U (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
     |ASM4 |NUP1 |NUP100 |NUP116 |NUP133 |NUP157 |NUP159 |NUP170 |NUP188 |NUP2 |NUP49 |NUP60 |POM152 |POM34
Mutants/Phenotypes
Strains/Constructs
Sharma K, et al. (1996) Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol Cell Biol 16(1):294-301
       |NUP1 |NUP100 |NUP116 |NUP133 |NUP145 |NUP49 |NUP85
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Simos G, et al. (1996) Nuclear pore proteins are involved in the biogenesis of functional tRNA. EMBO J 15(9):2270-84
     |LOS1 |NUP116 |NUP133 |NUP85 |PUS1 |PUS2
Reviews
Davis LI (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
     |ASM4 |NUP1 |NUP100 |NUP116 |NUP133 |NUP157 |NUP159 |NUP170 |NUP188 |NUP2 |NUP49 |NUP60 |POM152 |POM34
Protein-protein Interactions
Grandi P, et al. (1995) A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J Cell Biol 130(6):1263-73
     |NIC96 |NUP49 |NUP57 |NUP82
Cellular Location
Genetic Interactions
Mutants/Phenotypes
Protein-protein Interactions
Strains/Constructs
Grandi P, et al. (1995) Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p. EMBO J 14(1):76-87
     |NIC96 |NUP49 |NUP57
Function/Process
Mutants/Phenotypes
Strains/Constructs
Lim CR, et al. (1995) Thermosensitivity of green fluorescent protein fluorescence utilized to reveal novel nuclear-like compartments in a mutant nucleoporin NSP1. J Biochem 118(1):13-7

Fungal Related Genes/Proteins
Mutants/Phenotypes
Strains/Constructs
Schlaich NL and Hurt EC (1995) Analysis of nucleocytoplasmic transport and nuclear envelope structure in yeast disrupted for the gene encoding the nuclear pore protein Nup1p. Eur J Cell Biol 67(1):8-14
     |NUP1
Function/Process
Genetic Interactions
Fabre E, et al. (1994) Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif. Cell 78(2):275-89
       |NUP100 |NUP116 |NUP145
Cellular Location
Techniques and Reagents
Copeland CS and Snyder M (1993) Nuclear pore complex antigens delineate nuclear envelope dynamics in vegetative and conjugating Saccharomyces cerevisiae. Yeast 9(3):235-49
     |CDC10 |CDC11 |CDC12 |CDC14 |CDC15 |CDC3
Protein-protein Interactions
Grandi P, et al. (1993) Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J 12(8):3061-71
     |NIC96 |NUP49 |NUP57
Function/Process
Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Loeb JD, et al. (1993) NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex. Mol Biol Cell 4(2):209-22
     |NUP1 |NUP2
Function/Process
Mutants/Phenotypes
Strains/Constructs
Nehrbass U, et al. (1993) Analysis of nucleo-cytoplasmic transport in a thermosensitive mutant of nuclear pore protein NSP1. Eur J Cell Biol 62(1):1-12
     |HMLALPHA2 |MATALPHA2 |NOP1 |PHO2
Function/Process
Mutants/Phenotypes
Other Features
Strains/Constructs
Techniques and Reagents
Schlenstedt G, et al. (1993) Reconstitution of nuclear protein transport with semi-intact yeast cells. J Cell Biol 123(4):785-98
     |NPL3 |NUP49
Cellular Location
Mutants/Phenotypes
Strains/Constructs
Mutvei A, et al. (1992) NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation. Eur J Cell Biol 59(2):280-95
     |PHO2
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Wimmer C, et al. (1992) A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1. EMBO J 11(13):5051-61
     |NUP116 |NUP49
Non-Fungal Related Genes/Proteins
Carmo-Fonseca M, et al. (1991) Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Eur J Cell Biol 55(1):17-30

Fungal Related Genes/Proteins
Non-Fungal Related Genes/Proteins
Starr CM and Hanover JA (1991) A common structural motif in nuclear pore proteins (nucleoporins) Bioessays 13(3):145-6

Fungal Related Genes/Proteins
Davis LI and Fink GR (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
       |NUP1
Cellular Location
Protein Sequence Features
Strains/Constructs
Hurt EC (1990) Targeting of a cytosolic protein to the nuclear periphery. J Cell Biol 111(6 Pt 2):2829-37

Cellular Location
Function/Process
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Nehrbass U, et al. (1990) NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain. Cell 61(6):979-89
       |NOP1
Cell Cycle Phase Involved
Cellular Location
Mutants/Phenotypes
Strains/Constructs
Hurt EC (1989) NSP1, a yeast protein located at the nuclear periphery, is required for the cell cycle of Saccharomyces cerevisiae. J Cell Sci Suppl 12:243-52

Cell Cycle Phase Involved
Cellular Location
Protein Physical Properties
Protein Sequence Features
Techniques and Reagents
Hurt EC (1988) A novel nucleoskeletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae. EMBO J 7(13):4323-34

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SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.