SEC20/YDR498C Single Page Format |
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Contents
- Names and Identifiers
- GO Annotations
- Pathways
- Summary Paragraph
- Mutant Phenotypes
- Interactions
- Homologs
- Protein Info (physical properties, transcript info)
- PDB Homologs (protein structure info)
- Motifs
- Genome-wide Expression
(and other large-scale analyses)- Locus History (misc. notes)
- Sequence Retrieval and Analysis
- Map and Displays
- Localization
- Community Annotation
- Literature Guide
Sequence Coordinates
  ChrIV: 1446987 to 1445836
CDS: 1446987 - 1445836Click on map for expanded view
SGD ORF map GBrowse SGD Locus Page
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| Standard Name | Systematic Name | Alias | Feature Type | SGDID | ||||
| SEC20 | YDR498C |   | ORF, Verified | S000002906 | ||||
| Description | ||||||||
| Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p | ||||||||
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| No pathways available | |||||||||
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| No summary paragraph available | |||||||||
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| Phenotype page for SEC20/YDR498C | |||||||
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| Interaction page for SEC20/YDR498C | |||||||||
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MVVTFLQDLEVLQDALLNNLQKLSAISRRKESGESKHDNKDSFAAIANEH
NDEEEEIEFEDLVNIIESKVSDFESVLKCSIVEMTYKYPELKLQWEKSPR
YDQCDKLHIVKLDKQMNEDIYAQLVEELDFVLQFVDWFYCYRLKVKEILR
QHHKRDLAWNDEKRDRAIKFHAVDYDKLHQGTSSSSSLTSTSMEKASTRE
KLLSKTKQLTNNLVRGNQILQSGILQSDLNLDELRAQTNSLTQIDDKYTQ
FETVFKKTADLVKVLENASHQEKRDVYLSLGFLLCCVSWVLWRRIFKLPV
KLGLWLLFKFFKGILVTLGLVKSYAGSSSSLQAPSLVLNAPILATTTTSS
ATSVEPFASVSAVSSIQRAVDEAVDRIVSHDEL*
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| No protein structure information available. | |||||||||
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| You can also search multiple datasets simultaneously using Expression Connection for expression studies or Function Junction for other large scale analyses. | |||||
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| Nomenclature History |
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| Standard Name | Reference |
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| SEC20 | Novick P, et al. (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15 |
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| Sequence Type | Output Format | ||||
| Genomic DNA | GCG | FASTA | NoHeader | ||||
| Genomic DNA with 1 kb up and downstream | GCG | FASTA | NoHeader | ||||
| DNA coding sequence (without introns, without flanking regions) | GCG | FASTA | NoHeader | ||||
| Protein Translation of ORF | GCG | FASTA | NoHeader | ||||
| 6-Frame Translation(with Restriction Map) | GCG | ||||
| Restriction Fragment Sizes | GCG | ||||
| Sequence from other databases | |
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| Sequence ID | Source |
| YDR498C | SGD Systematic Sequence |
| 852109 | NCBI: Gene ID |
| NP_010786.1 | NCBI: RefSeq protein version ID |
| NP_010786.1 | NCBI: RefSeq protein version ID |
| 6320706 | NCBI: NCBI protein GI |
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| Physical, Genetic Maps: | Chromosomal Feature Map | GBrowse | Combined Physical and Genetic Map | Genetic Distance vs. Physical Distance Ratios | ||||
| Similarity Viewers: | Synteny Viewer | Genomic Stripe View | SAGE Results Map |   | ||||
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| No community annotation available. | |||||||||
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| Topics | Reference | Other Genes Addressed | 44 curated references; 0 references not yet curated | |||
| Reviews | Nagotu S, et al. (2010) Divide et impera: the dictum of peroxisomes. Traffic 11(2):175-84 | |ARF1 |ARF3 |CAF4 |DNM1 |DSL1 |EMP24 |FIS1 |MDV1 |PEX11 |PEX2 |PEX3 |PEX30 |PEX31 |SEC39 |MORE | ||||
| Cellular Location Function/Process Mutants/Phenotypes Protein Processing/Modification/Regulation Strains/Constructs | Perry RJ, et al. (2009) Endoplasmic Reticulum-Associated Secretory Proteins Sec20p, Sec39p, and Dsl1p Are Involved in Peroxisome Biogenesis. Eukaryot Cell 8(6):830-843 | |BOS1 |COP1 |DSL1 |PEX3 |POT1 |SEC1 |SEC11 |SEC12 |SEC13 |SEC14 |SEC17 |SEC18 |SEC21 |SEC26 |MORE | ||||
| Protein Sequence Features Protein-protein Interactions | Ren Y, et al. (2009) A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1. Cell 139(6):1119-29 ![]() | |DSL1 |EXO70 |SEC17 |SEC22 |SEC39 |TIP20 |UFE1 |USE1 | ||||
| Reviews | Saito C and Ueda T (2009) Chapter 4 Functions of RAB and SNARE Proteins in Plant Life. Int Rev Cell Mol Biol 274:183-233 | |MSO1 |PEP12 |PEP3 |PEP5 |PEP7 |SAR1 |SEC1 |SEC2 |SEC4 |SEC9 |SFT1 |SNC1 |SSO1 |USE1 |MORE | ||||
| Reviews | Schmitt HD and Jahn R (2009) A tethering complex recruits SNAREs and grabs vesicles. Cell 139(6):1053-5 | |BET3 |BET5 |DSL1 |GSG1 |SEC1 |SEC23 |SEC39 |TIP20 |TRS20 |TRS23 |TRS31 |TRS33 |USE1 | ||||
| Mutants/Phenotypes Strains/Constructs | Singh J and Tyers M (2009) A Rab escort protein integrates the secretion system with TOR signaling and ribosome biogenesis. Genes Dev 23(16):1944-58 | |AKR1 |AVL9 |DPM1 |ERG5 |ERV29 |GAB1 |GPI16 |MRS6 |MSB4 |MSN5 |PKC1 |ROT1 |SEC17 |SEC23 |MORE | ||||
| Genetic Interactions Mutants/Phenotypes Protein-protein Interactions Strains/Constructs | Tripathi A, et al. (2009) Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol 16(2):114-23 | |DSL1 |EXO70 |EXO84 |SEC15 |SEC39 |SEC6 |TIP20 |USE1 | ||||
| Mutants/Phenotypes Strains/Constructs | Ungar L, et al. (2009) A genome-wide screen for essential yeast genes that affect telomere length maintenance. Nucleic Acids Res 37(12):3840-9 | |AAR2 |ALA1 |APC4 |ARC15 |ARC35 |ARP2 |ARP3 |BBP1 |CBF5 |CDC13 |CDC16 |CDC19 |CDC34 |CDC36 |MORE | ||||
| Mutants/Phenotypes Strains/Constructs | Breslow DK, et al. (2008) A comprehensive strategy enabling high-resolution functional analysis of the yeast genome. Nat Methods 5(8):711-8 | |AAR2 |ABD1 |ABF1 |ACC1 |ACP1 |ADE13 |AFG2 |ALA1 |ALG1 |ALG13 |ALG14 |ALG2 |ALG7 |ALR1 |MORE | ||||
| Genetic Interactions | Higashio H, et al. (2008) Smy2p Participates in COPII Vesicle Formation Through the Interaction with Sec23p/Sec24p Subcomplex. Traffic 9(1):79-93 | |BET1 |COG2 |COG3 |RET1 |RET3 |SAR1 |SEC12 |SEC13 |SEC16 |SEC17 |SEC18 |SEC21 |SEC22 |SEC23 |MORE | ||||
| Evolution Fungal Related Genes/Proteins Non-Fungal Related Genes/Proteins | Koumandou VL, et al. (2007) Control systems for membrane fusion in the ancestral eukaryote; evolution of tethering complexes and SM proteins. BMC Evol Biol 7():29 | |BET3 |BET5 |COG1 |COG2 |COG3 |COG4 |COG5 |COG6 |COG7 |COG8 |DSL1 |EXO70 |EXO84 |GSG1 |MORE | ||||
| Fungal Related Genes/Proteins | Kuratsu M, et al. (2007) Systematic analysis of SNARE localization in the filamentous fungus Aspergillus oryzae. Fungal Genet Biol 44(12):1310-23 | |BET1 |BOS1 |GOS1 |NYV1 |PEP12 |SEC22 |SEC9 |SED5 |SFT1 |SNC1 |SNC2 |SPO20 |SSO1 |SSO2 |MORE | ||||
| Function/Process Mutants/Phenotypes Strains/Constructs | Altmann K and Westermann B (2005) Role of essential genes in mitochondrial morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell 16(11):5410-7 | |ARC35 |ARC40 |ARP2 |BFR2 |CCT4 |CCT6 |CDC34 |CDC53 |COF1 |DSL1 |ERG1 |ERG10 |ERG12 |ERG13 |MORE | ||||
| Reviews | Hong W (2005) SNAREs and traffic. Biochim Biophys Acta 1744(2):120-44 | |BET1 |BOS1 |GOS1 |NYV1 |PEP12 |SEC22 |SEC9 |SED5 |SFT1 |SNC1 |SNC2 |SPO20 |SSO1 |SSO2 |MORE | ||||
| Cellular Location Protein-protein Interactions | Kraynack BA, et al. (2005) Dsl1p, Tip20p, and the novel Dsl3(Sec39) protein are required for the stability of the Q/t-SNARE complex at the endoplasmic reticulum in yeast. Mol Biol Cell 16(9):3963-77 | |DSL1 |SEC39 |TIP20 |UFE1 |USE1 | ||||
| Genetic Interactions Mutants/Phenotypes Protein-protein Interactions Strains/Constructs | Li Y, et al. (2005) Structure-based functional analysis reveals a role for the SM protein Sly1p in retrograde transport to the endoplasmic reticulum. Mol Biol Cell 16(9):3951-62 | |BOS1 |DSL1 |SEC22 |SLY1 |TIP20 |UFE1 |USE1 | ||||
| Genetic Interactions Mutants/Phenotypes Strains/Constructs | Routt SM, et al. (2005) Nonclassical PITPs activate PLD via the Stt4p PtdIns-4-kinase and modulate function of late stages of exocytosis in vegetative yeast. Traffic 6(12):1157-72 | |CSR1 |LSB6 |MSS4 |PDR16 |PDR17 |PIK1 |PSD1 |SAC1 |SEC1 |SEC10 |SEC12 |SEC13 |SEC14 |SEC15 |MORE | ||||
| Cellular Location Function/Process Fungal Related Genes/Proteins Protein Sequence Features Reviews | Burri L and Lithgow T (2004) A complete set of SNAREs in yeast. Traffic 5(1):45-52 | |BET1 |BOS1 |FRT1 |FRT2 |GOS1 |NYV1 |PEP12 |SEC22 |SEC9 |SED5 |SFT1 |SNC1 |SNC2 |SSO1 |MORE | ||||
| Non-Fungal Related Genes/Proteins | Nakajima K, et al. (2004) Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion. EMBO J 23(16):3216-26 | |||||
| Function/Process Protein Sequence Features Protein-protein Interactions | Burri L, et al. (2003) A SNARE required for retrograde transport to the endoplasmic reticulum. Proc Natl Acad Sci U S A 100(17):9873-7 | |SEC22 |UFE1 |USE1 |YKT6 | ||||
| Function/Process Genetic Interactions Mutants/Phenotypes Protein-protein Interactions Strains/Constructs | Dilcher M, et al. (2003) Use1p is a yeast SNARE protein required for retrograde traffic to the ER. EMBO J 22(14):3664-74 | |BET1 |BOS1 |SEC22 |SED5 |UFE1 |USE1 | ||||
| Fungal Related Genes/Proteins | Weber Y, et al. (2002) Sec20p-interacting proteins (Tip20p, Ufe1p) in the retrograde secretory pathway of the fungal pathogen Candida albicans. Mol Genet Genomics 268(4):468-76 | |TIP20 |UFE1 | ||||
| Cellular Location Function/Process Genetic Interactions | Andag U, et al. (2001) The coatomer-interacting protein Dsl1p is required for Golgi-to-endoplasmic reticulum retrieval in yeast. J Biol Chem 276(42):39150-60 | |COP1 |DSL1 |ERD2 |KAR2 |SEC22 |TIP20 | ||||
| Cellular Location Function/Process Protein Physical Properties Protein-protein Interactions | Reilly BA, et al. (2001) Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit. Mol Biol Cell 12(12):3783-96 | |DSL1 |KAR2 |RET2 |SLY1 |TIP20 |UFE1 | ||||
| Function/Process Mutants/Phenotypes Strains/Constructs | Schleip I, et al. (2001) The yeast SEC20 gene is required for N- and O-glycosylation in the Golgi. Evidence that impaired glycosylation does not correlate with the secretory defect. J Biol Chem 276(31):28751-8 | |||||
| Fungal Related Genes/Proteins Protein Sequence Features | Weber Y, et al. (2001) Divergence of eukaryotic secretory components: the Candida albicans homolog of the Saccharomyces cerevisiae ++Sec20 protein is N terminally truncated, and its levels determine antifungal drug resistance and growth. J Bacteriol 183(1):46-54 | |TIP20 | ||||
| Function/Process Genetic Interactions Mutants/Phenotypes Strains/Constructs | Finger FP and Novick P (2000) Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics 156(3):943-51 | |ACT1 |CDC12 |CDC24 |CDC28 |CDC42 |DSS4 |GDI1 |MYO2 |SEC1 |SEC10 |SEC12 |SEC13 |SEC14 |SEC15 |MORE | ||||
| Reviews | Pelham HR (1999) SNAREs and the secretory pathway-lessons from yeast. Exp Cell Res 247(1):1-8 | |GOS1 |NYV1 |SEC22 |SEC9 |SED5 |SNC1 |SNC2 |SPO20 |SSO1 |SSO2 |TLG1 |TLG2 |UFE1 |VAM3 |MORE | ||||
| Function/Process Genetic Interactions Mutants/Phenotypes Strains/Constructs Substrates/Ligands/Cofactors | Ballensiefen W, et al. (1998) Recycling of the yeast v-SNARE Sec22p involves COPI-proteins and the ER transmembrane proteins Ufe1p and Sec20p. J Cell Sci 111 ( Pt 11):1507-20 | |SEC21 |SEC22 |SEC27 |UFE1 | ||||
| Cellular Location Function/Process Genetic Interactions Mutants/Phenotypes Protein-protein Interactions Strains/Constructs | Frigerio G (1998) The Saccharomyces cerevisiae early secretion mutant tip20 is synthetic lethal with mutants in yeast coatomer and the SNARE proteins Sec22p and Ufe1p. Yeast 14(7):633-46 | |RET2 |SEC21 |SEC22 |SEC27 |TIP20 |UFE1 | ||||
| Mutants/Phenotypes Protein-protein Interactions Strains/Constructs | Patel SK, et al. (1998) Organelle membrane fusion: a novel function for the syntaxin homolog Ufe1p in ER membrane fusion. Cell 92(5):611-20 | |CDC48 |TIP20 |UFE1 | ||||
| Function/Process Protein-protein Interactions | Cosson P, et al. (1997) The Sec20/Tip20p complex is involved in ER retrieval of dilysine-tagged proteins. Eur J Cell Biol 73(2):93-7 | |EMP47 |TIP20 | ||||
| Cellular Location Function/Process Genetic Interactions Mutants/Phenotypes | Lewis MJ, et al. (1997) A novel SNARE complex implicated in vesicle fusion with the endoplasmic reticulum. EMBO J 16(11):3017-24 | |SEC22 |TIP20 |UFE1 | ||||
| Function/Process Mutants/Phenotypes Strains/Constructs | Shimma Y, et al. (1997) A defect in GTP synthesis affects mannose outer chain elongation in Saccharomyces cerevisiae. Mol Gen Genet 256(5):469-80 | |ALG1 |GUK1 |PSA1 | ||||
| Protein-protein Interactions | Voet M, et al. (1997) The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames. Yeast 13(2):177-82 | |ARO2 |FLC3 |GPI10 |HUL5 |MRF1 |ROG1 |RPL9A |RRT6 |TIP20 |YGL140C | ||||
| Mutants/Phenotypes Regulatory Role | Lewis MJ and Pelham HR (1996) SNARE-mediated retrograde traffic from the Golgi complex to the endoplasmic reticulum. Cell 85(2):205-15 | |ERD2 |SEC21 |UFE1 | ||||
| Function/Process Genetic Interactions Protein-protein Interactions Regulatory Role | Sweet DJ and Pelham HR (1993) The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kDa cytoplasmic protein that interacts with the cytoplasmic domain of Sec20p. EMBO J 12(7):2831-40 | |TIP20 | ||||
| Reviews | Pryer NK, et al. (1992) Vesicle-mediated protein sorting. Annu Rev Biochem 61:471-516 | |ARF1 |ARF2 |BET1 |BET2 |BOS1 |CHC1 |CLC1 |ERD1 |ERD2 |SAR1 |SEC1 |SEC12 |SEC13 |SEC14 |MORE | ||||
| Cellular Location DNA/RNA Sequence Features Function/Process Protein Processing/Modification/Regulation Protein Sequence Features Regulatory Role Strains/Constructs | Sweet DJ and Pelham HR (1992) The Saccharomyces cerevisiae SEC20 gene encodes a membrane glycoprotein which is sorted by the HDEL retrieval system. EMBO J 11(2):423-32 | |||||
| Function/Process Mutants/Phenotypes | Puoti A, et al. (1991) Biosynthesis of mannosylinositolphosphoceramide in Saccharomyces cerevisiae is dependent on genes controlling the flow of secretory vesicles from the endoplasmic reticulum to the Golgi. J Cell Biol 113(3):515-25 | |SEC12 |SEC13 |SEC14 |SEC16 |SEC17 |SEC18 |SEC21 |SEC22 |SEC23 |SEC6 |SEC7 | ||||
| Reviews | Newman AP and Ferro-Novick S (1990) Defining components required for transport from the ER to the Golgi complex in yeast. Bioessays 12(10):485-91 | |ARF1 |ARF2 |BET1 |BET2 |BOS1 |SAR1 |SEC12 |SEC13 |SEC16 |SEC17 |SEC18 |SEC21 |SEC22 |SEC23 |MORE | ||||
| Function/Process Mutants/Phenotypes Strains/Constructs | Ramirez RM, et al. (1983) Plasma membrane expansion terminates in Saccharomyces cerevisiae secretion-defective mutants while phospholipid synthesis continues. J Bacteriol 154(3):1276-83 | |GDI1 |SEC1 |SEC10 |SEC11 |SEC12 |SEC13 |SEC14 |SEC15 |SEC16 |SEC17 |SEC18 |SEC2 |SEC21 |SEC22 |MORE | ||||
| Function/Process Genetic Interactions Mutants/Phenotypes Strains/Constructs | Novick P, et al. (1981) Order of events in the yeast secretory pathway. Cell 25(2):461-9 | |GDI1 |SEC1 |SEC12 |SEC14 |SEC15 |SEC16 |SEC18 |SEC2 |SEC21 |SEC22 |SEC23 |SEC3 |SEC4 |SEC5 |MORE | ||||
| Function/Process Genetic Interactions Mutants/Phenotypes Strains/Constructs | Novick P, et al. (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15 | |GDI1 |SEC1 |SEC10 |SEC11 |SEC12 |SEC13 |SEC14 |SEC15 |SEC16 |SEC17 |SEC18 |SEC2 |SEC21 |SEC22 |MORE | ||||
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