PET9/YBL030C Single Page Format

This page provides an alternative format to the SGD Locus Summary Page. Note that additional information may be available on or linked from the standard format SGD Locus Summary page.

Contents
Names and Identifiers

GO Annotations

Pathways

Summary Paragraph

Mutant Phenotypes

Interactions

Homologs

Protein Info (physical properties, transcript info)

PDB Homologs (protein structure info)

Motifs

Genome-wide Expression
(and other large-scale analyses)

Locus History (misc. notes)

Sequence Retrieval and Analysis

Map and Displays

Localization

Community Annotation

Literature Guide

Sequence Coordinates

ChrII: 164000 to 163044
CDS: 164000 - 163044
Genetic position: -20 cM
Genetic Map Data

Click on map for expanded view

SGD ORF map GBrowse
SGD Locus Page

Names and Identifiers [TOP] [NEXT]
Standard Name Systematic Name Alias Feature Type SGDID

PET9 YBL030C AAC2, ANC2, OP1 ORF, Verified S000000126

Description
Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; required for viability in many common lab strains carrying a mutation in the polymorphic SAL1 gene

GO Annotations [TOP] [NEXT]
Molecular Function
Annotation(s) Reference(s) Evidence Assigned By
ATP:ADP antiporter activity Brandolin G, et al. (1993) Biochemical characterisation of the isolated Anc2 adenine nucleotide carrier from Saccharomyces cerevisiae mitochondria. Biochem Biophys Res Commun 192(1):143-50
     IDA : Inferred from Direct Assay
Assigned on 2001-02-13 SGD
binding DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR001993 , EBI:IPR002067
Assigned on 2007-05-23 UniProtKB
substrate-specific transmembrane transporter activity Tian W, et al. (2008) Combining guilt-by-association and guilt-by-profiling to predict Saccharomyces cerevisiae gene function. Genome Biol 9 Suppl 1:S7
             RCA : Reviewed Computational Analysis
Assigned on 2009-09-03 YeastFunc
transporter activity DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR002113
Assigned on 2007-05-23 UniProtKB
Biological Process
Annotation(s) Reference(s) Evidence Assigned By
ADP transport Brandolin G, et al. (1993) Biochemical characterisation of the isolated Anc2 adenine nucleotide carrier from Saccharomyces cerevisiae mitochondria. Biochem Biophys Res Commun 192(1):143-50
     IDA : Inferred from Direct Assay
Assigned on 2007-03-08 SGD
ATP transport Brandolin G, et al. (1993) Biochemical characterisation of the isolated Anc2 adenine nucleotide carrier from Saccharomyces cerevisiae mitochondria. Biochem Biophys Res Commun 192(1):143-50
     IDA : Inferred from Direct Assay
Assigned on 2007-03-08 SGD
aerobic respiration Gawaz M, et al. (1990) Structure-function studies of adenine nucleotide transport in mitochondria. II. Biochemical analysis of distinct AAC1 and AAC2 proteins in yeast. J Biol Chem 265(24):14202-8
     IMP : Inferred from Mutant Phenotype
Assigned on 2004-02-26 SGD
anaerobic respiration Drgon T, et al. (1991) ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae. FEBS Lett 289(2):159-62
     IGI : Inferred from Genetic Interaction with SGD:AAC3
Assigned on 2004-02-26 SGD
apoptosis Pereira C, et al. (2007) ADP/ATP carrier is required for mitochondrial outer membrane permeabilization and cytochrome c release in yeast apoptosis. Mol Microbiol 66(3):571-82
         IMP : Inferred from Mutant Phenotype
Assigned on 2009-10-09 SGD
heme biosynthetic process Azuma M, et al. (2008) Adenine nucleotide translocator transports haem precursors into mitochondria. PLoS ONE 3(8):e3070
         IGI : Inferred from Genetic Interaction with SGD:AAC1, SGD:AAC3
Assigned on 2008-09-09 SGD
ion transport Huttenhower C and Troyanskaya OG (2009) Prediction of Gene Ontology annotations by integrating high-throughput datasets
   RCA : Reviewed Computational Analysis
Assigned on 2009-08-06 bioPIXIE_MEFIT
mitochondrial transport Gawaz M, et al. (1990) Structure-function studies of adenine nucleotide transport in mitochondria. II. Biochemical analysis of distinct AAC1 and AAC2 proteins in yeast. J Biol Chem 265(24):14202-8
     IMP : Inferred from Mutant Phenotype
Assigned on 2004-02-26 SGD
nucleotide metabolic process Huttenhower C and Troyanskaya OG (2009) Prediction of Gene Ontology annotations by integrating high-throughput datasets
   RCA : Reviewed Computational Analysis
Assigned on 2009-08-06 bioPIXIE_MEFIT
oxidation reduction Huttenhower C and Troyanskaya OG (2009) Prediction of Gene Ontology annotations by integrating high-throughput datasets
   RCA : Reviewed Computational Analysis
Assigned on 2009-08-06 bioPIXIE_MEFIT
transmembrane transport Brandolin G, et al. (1993) Biochemical characterisation of the isolated Anc2 adenine nucleotide carrier from Saccharomyces cerevisiae mitochondria. Biochem Biophys Res Commun 192(1):143-50
     IDA : Inferred from Direct Assay
Assigned on 2008-12-09 SGD
DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR002067 , EBI:IPR002113 , EBI:IPR001993
Assigned on 2009-10-01 UniProtKB
transport DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR001993 , EBI:IPR002067 , EBI:IPR002113
Assigned on 2007-05-23 UniProtKB
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0813
Assigned on 2007-05-23 UniProtKB
Cellular Component
Annotation(s) Reference(s) Evidence Assigned By
integral to membrane GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0812
Assigned on 2007-05-23 UniProtKB
GOA curators and UniProt curators (2007) Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping.
     IEA : Inferred from Electronic Annotation with EBI:SL-9909
Assigned on 2009-10-01 UniProtKB
membrane DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR001993
Assigned on 2007-05-23 UniProtKB
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0472
Assigned on 2007-05-23 UniProtKB
mitochondrial inner membrane Lauquin G, et al. (1976) Effect of genetic and physiological manipulations onthe kinetic and binding parameters of the adenine nucleotide translocator in Saccharomyces cervisiae and Candida utilis. Biochimie 58(10):1213-20
     IDA : Inferred from Direct Assay
Assigned on 2001-01-18 SGD
DDB, et al. (2001) Gene Ontology annotation through association of InterPro records with GO terms.
     IEA : Inferred from Electronic Annotation with EBI:IPR002067 , EBI:IPR002113
Assigned on 2009-06-17 UniProtKB
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0999
Assigned on 2009-06-29 UniProtKB
mitochondrion Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
           IDA : Inferred from Direct Assay
Assigned on 2006-12-12 SGD
Brandina I, et al. (2006) Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta 1757(9-10):1217-1228
       IPI : Inferred from Physical Interaction with SGD:ENO1, SGD:ENO2
Assigned on 2007-03-05 SGD
GOA curators (2000) Gene Ontology annotation based on Swiss-Prot keyword mapping.
     IEA : Inferred from Electronic Annotation with EBI:KW-0496
Assigned on 2007-05-23 UniProtKB

Pathways [TOP] [NEXT]
No pathways available

Summary Paragraph [TOP] [NEXT]
No summary paragraph available

Basic References [TOP]
BASIC INFORMATION REFERENCES forPET9/YBL030C for PET9
1) Adrian GS, et al. (1986) Sequences required for delivery and localization of the ADP/ATP translocator to the mitochondrial inner membrane. Mol Cell Biol 6(2):626-34

2) Chen XJ (2004) Sal1p, a calcium-dependent carrier protein that suppresses an essential cellular function associated With the Aac2 isoform of ADP/ATP translocase in Saccharomyces cerevisiae. Genetics 167(2):607-17

3) Reinders J, et al. (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906

Mutant Phenotypes [TOP] [NEXT]
Phenotype page for PET9/YBL030C

Interactions: genetic, physical, and other gene-gene links. [TOP] [NEXT]
Interaction page for PET9/YBL030C

Homologs [TOP] [NEXT]

Comparison Resources

Physical Properties and Transcript Information: predicted from sequence [TOP] [NEXT]
Protein Sequence Calculations
from Predicted Full length Translation

N-term MSSNAQV

C-term LFGKKFK

Length(aa) 318

MW(Da) 34,426

pI 10.61

Amino Acid Composition (full length)

GCG tools: PepPlot, Helical Wheel, PepStruct

Transcript Translation Calculations

Codon Bias 0.622

Codon Adaptation Index 0.538

Frequency of Optimal Codons 0.764

Hydropathicity of Protein 0.086

Aromaticity Score 0.113

10 20 30 40 50 | | | | | MSSNAQVKTPLPPAPAPKKESNFLIDFLMGGVSAAVAKTAASPIERVKLL IQNQDEMLKQGTLDRKYAGILDCFKRTATQEGVISFWRGNTANVIRYFPT QALNFAFKDKIKAMFGFKKEEGYAKWFAGNLASGGAAGALSLLFVYSLDY ARTRLAADSKSSKKGGARQFNGLIDVYKKTLKSDGVAGLYRGFLPSVVGI VVYRGLYFGMYDSLKPLLLTGSLEGSFLASFLLGWVVTTGASTCSYPLDT VRRRMMMTSGQAVKYDGAFDCLRKIVAAEGVGSLFKGCGANILRGVAGAG VISMYDQLQMILFGKKFK*

Protein Structures from PDB: proteins of known structure with sequence similarity to PET9/YBL030C, based on Smith-Waterman analysis. [TOP] [NEXT]
PDB protein structure(s) homologous to PET9 Homolog Source (per PDB) Protein Alignment: PET9 vs. Homolog External Links
P-Value %Identical %Similar Alignment
1okc ( Chain: A)
Structure of mitochondrial adp/atp carrier in complex with carboxyatractyloside
PDB_Info
PDB_Structure
Bos taurus 1.0e-65 53 22 View alignment SCOP
MMDB
CATH
2c3e ( Chain: A)
The bovine mitochondrial adp-atp carrier
PDB_Info
PDB_Structure
Bos taurus 1.0e-65 53 22 View alignment SCOP
MMDB
CATH

Genome-wide Expression and Other Large-Scale Analyses [TOP] [NEXT]

Functional Analysis
You can also search multiple datasets simultaneously using Expression Connection for expression studies or Function Junction for other large scale analyses.

Locus History (misc. notes) [TOP] [NEXT]
Nomenclature History
Standard Name Reference
PET9 SGD (2007) Information without a citation in SGD

Alias Name(s) Reference
AAC2 Lawson JE and Douglas MG (1988) Separate genes encode functionally equivalent ADP/ATP carrier proteins in Saccharomyces cerevisiae. Isolation and analysis of AAC2. J Biol Chem 263(29):14812-8

ANC2 Brandolin G, et al. (1993) Biochemical characterisation of the isolated Anc2 adenine nucleotide carrier from Saccharomyces cerevisiae mitochondria. Biochem Biophys Res Commun 192(1):143-50

OP1 Kovac L, et al. (1967) Biochemical genetics of oxidative phosphorylation. Science 158(808):1564-7

Mapping Notes
Date Note
1998-11-10 Edition 15: The name ANC2, a non-standard name for pet9, has also been used to refer to CCT4, the cytoplasmic chaperonin subunit that was originally identified as an actin non-complement mutant (ANC).
Cherry JM, et al. (1998) "Genetic and Physical Maps of Saccharomyces cerevisiae (Edition 15)". Pp. 414-420 in 1998 Yeast Genetics and Molecular Biology Meeting Program and Abstracts. Bethesda, MD: The Genetics Society of America

1997-10-20 Edition 14: pet9 is often referred to as op1
Cherry JM, et al. (1997) Genetic and physical maps of Saccharomyces cerevisiae. Nature 387(6632 Suppl):67-73

Standard Name	Reference
PET9	SGD (2007) Information without a citation in SGD

Alias Name(s)	Reference
AAC2	Lawson JE and Douglas MG (1988) Separate genes encode functionally equivalent ADP/ATP carrier proteins in Saccharomyces cerevisiae. Isolation and analysis of AAC2. J Biol Chem 263(29):14812-8
ANC2	*Brandolin G, et al.* (1993)** Biochemical characterisation of the isolated Anc2 adenine nucleotide carrier from Saccharomyces cerevisiae mitochondria. Biochem Biophys Res Commun 192(1):143-50
OP1	*Kovac L, et al.* (1967)** Biochemical genetics of oxidative phosphorylation. Science 158(808):1564-7

Date	Note
1998-11-10	Edition 15: The name ANC2, a non-standard name for pet9, has also been used to refer to CCT4, the cytoplasmic chaperonin subunit that was originally identified as an actin non-complement mutant (ANC). *Cherry JM, et al.* (1998)** "Genetic and Physical Maps of Saccharomyces cerevisiae (Edition 15)". Pp. 414-420 in 1998 Yeast Genetics and Molecular Biology Meeting Program and Abstracts. Bethesda, MD: The Genetics Society of America
1997-10-20	Edition 14: pet9 is often referred to as op1 *Cherry JM, et al.* (1997)** Genetic and physical maps of Saccharomyces cerevisiae. Nature 387(6632 Suppl):67-73

Sequence Retrieval [TOP] [NEXT]
Sequence Type Output Format

Genomic DNA GCG | FASTA | NoHeader

Genomic DNA with 1 kb up and downstream GCG | FASTA | NoHeader

DNA coding sequence
(without introns, without flanking regions) GCG | FASTA | NoHeader

Protein Translation of ORF GCG | FASTA | NoHeader

6-Frame Translation(with Restriction Map) GCG

Restriction Fragment Sizes GCG

Sequence Analysis Tools
Sequence from other databases

Sequence ID Source

YBL030C SGD Systematic Sequence

852250 NCBI: Gene ID

NP_009523.1 NCBI: RefSeq protein version ID

NP_009523.1 NCBI: RefSeq protein version ID

6319441 NCBI: NCBI protein GI

Sequence from other databases
Sequence ID	Source
YBL030C	SGD Systematic Sequence
852250	NCBI: Gene ID
NP_009523.1	NCBI: RefSeq protein version ID
NP_009523.1	NCBI: RefSeq protein version ID
6319441	NCBI: NCBI protein GI

Map and Displays [TOP] [NEXT]
Physical, Genetic Maps: Chromosomal Feature Map GBrowse Combined Physical and Genetic Map Genetic Distance vs. Physical Distance Ratios
Similarity Viewers: Synteny Viewer Genomic Stripe View SAGE Results Map

Localization [TOP] [NEXT]

Localization Resources

Community Annotation [TOP] [NEXT]
No community annotation available.

Literature Guide: papers categorized by topic. [TOP]
Topics Reference Other Genes Addressed
145 curated references; 0 references not yet curated
Techniques and Reagents
Amutha B, et al. (2009) Chapter 14 Nucleotide-dependent iron-sulfur cluster biogenesis of endogenous and imported apoproteins in isolated intact mitochondria. Methods Enzymol 456:247-66
       |ACO1 |GGC1 |NFS1 |YAH1
Large-scale protein detection
Techniques and Reagents
Braun RJ, et al. (2009) 16-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis. Methods Mol Biol 528:83-107
       |ACO1 |ALO1 |ATP1 |ATP2 |ATP7 |HSP60 |IDH2 |ILV5 |KGD2 |NDI1 |PDB1 |POR1 |RIP1 |SSC1
Alias
Cross-species Expression
Genetic Interactions
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Feng J, et al. (2009) Tyrosine phosphorylation by Src within the cavity of the adenine nucleotide translocase 1 regulates ADP/ATP exchange in mitochondria. Am J Physiol Cell Physiol
       |AAC1 |AAC3
Disease Gene Related
Techniques and Reagents
Fontanesi F, et al. (2009) Evaluation of the mitochondrial respiratory chain and oxidative phosphorylation system using yeast models of OXPHOS deficiencies. Curr Protoc Hum Genet Chapter 19:Unit19.5
       |ATM1 |ATP6 |BCS1 |COX12 |EMI5 |ERV1 |LSC1 |MEF1 |MIP1 |MIR1 |ORT1 |PIC2 |SDH2 |SOD1 |MORE
Cell Cycle Phase Involved
Regulation of
Goldberg AA, et al. (2009) Effect of calorie restriction on the metabolic history of chronologically aging yeast. Exp Gerontol 44(9):555-71
       |ABF2 |ACO1 |ACT1 |ADH1 |ADH2 |ALD4 |ARE2 |ATH1 |ATP1 |ATP2 |AYR1 |CAF4 |CCP1 |CIT1 |MORE
Techniques and Reagents
Gubbens J, et al. (2009) Photocrosslinking and click chemistry enable the specific detection of proteins interacting with phospholipids at the membrane interface. Chem Biol 16(1):3-14
       |ACO1 |ALD4 |ATP1 |ATP17 |ATP18 |ATP2 |ATP4 |ATP7 |CHC1 |COR1 |COX2 |COX4 |COX9 |COY1 |MORE
Function/Process
Mutants/Phenotypes
Protein Physical Properties
Protein Sequence Features
Ohkura K, et al. (2009) Role of C-terminal region of yeast ADP/ATP carrier 2 protein: dynamics of flexible C-terminal arm. Anticancer Res 29(11):4897-900

Function/Process
Olsen LF, et al. (2009) Regulation of glycolytic oscillations by mitochondrial and plasma membrane H+-ATPases. Biophys J 96(9):3850-61
       |ATP1 |ATP14 |ATP15 |ATP16 |ATP17 |ATP18 |ATP19 |ATP2 |ATP20 |ATP3 |ATP4 |ATP5 |ATP6 |ATP7 |MORE
Regulation of
Transcription
Peiro-Chova L and Estruch F (2009) The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes. J Biol Chem 284(42):28958-67
       |ARG1 |ARG5,6 |ATP15 |ATP16 |ATP17 |ATP19 |ATP3 |ATP7 |BUR6 |COX13 |COX7 |GDH1 |HAP4 |HSP78 |MORE
Strains/Constructs
Techniques and Reagents
Stuart RA (2009) Chapter 11 Supercomplex organization of the yeast respiratory chain complexes and the ADP/ATP carrier proteins. Methods Enzymol 456:191-208
       |COR1 |COX14 |COX2 |COX5A |QCR2 |SHY1 |TIM17 |TIM23
Evolution
Non-Fungal Related Genes/Proteins
Szklarczyk R and Huynen MA (2009) Expansion of the human mitochondrial proteome by intra- and inter-compartmental protein duplication. Genome Biol 10(11):R135
       |AAC1 |AAC3 |AAT2 |ABC1 |ACN9 |ACO1 |ACP1 |ADK1 |ADK2 |AGC1 |AHP1 |ALD4 |ALD5 |ALD6 |MORE
Reviews
Traba J, et al. (2009) Transport of adenine nucleotides in the mitochondria of Saccharomyces cerevisiae: Interactions between the ADP/ATP carriers and the ATP-Mg/Pi carrier. Mitochondrion 9(2):79-85

Protein-protein Interactions
Alcock FH, et al. (2008) Conserved substrate binding by chaperones in the bacterial periplasm and the mitochondrial intermembrane space. Biochem J 409(2):377-87
       |POR1 |TIM10 |TIM9 |TOM40
Alias
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Azuma M, et al. (2008) Adenine nucleotide translocator transports haem precursors into mitochondria. PLoS ONE 3(8):e3070
         |AAC1 |AAC3 |ATP3 |CTA1
Mutants/Phenotypes
Strains/Constructs
Breslow DK, et al. (2008) A comprehensive strategy enabling high-resolution functional analysis of the yeast genome. Nat Methods 5(8):711-8
       |AAR2 |ABD1 |ABF1 |ACC1 |ACP1 |ADE13 |AFG2 |ALA1 |ALG1 |ALG13 |ALG14 |ALG2 |ALG7 |ALR1 |MORE
Alias
Function/Process
Genetic Interactions
Mutants/Phenotypes
Protein-protein Interactions
Regulation of
Strains/Constructs
Claypool SM, et al. (2008) Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane. J Cell Biol 182(5):937-50
       |AAC1 |ADH3 |COB |COR1 |COX12 |COX13 |COX2 |COX4 |COX5A |COX6 |CRD1 |CYC1 |CYT1 |DIC1 |MORE
Protein-protein Interactions
Strains/Constructs
Claypool SM, et al. (2008) The cardiolipin transacylase, tafazzin, associates with two distinct respiratory components providing insight into Barth syndrome. Mol Biol Cell 19(12):5143-55
       |ATP1 |ATP2 |CRD1 |TAZ1
Function/Process
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Substrates/Ligands/Cofactors
Clemencon B, et al. (2008) Structure-Function Relationships of the C-Terminal End of the Saccharomyces cerevisiae ADP/ATP Carrier Isoform 2. J Biol Chem 283(17):11218-11225

Alias
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Protein Physical Properties
Protein Processing/Modification/Regulation
Protein/Nucleic Acid Structure
Strains/Constructs
Substrates/Ligands/Cofactors
David C, et al. (2008) Two residues of a conserved aromatic ladder of the mitochondrial ADP/ATP carrier are crucial to nucleotide transport. Biochemistry 47(50):13223-31

Protein-protein Interactions
Strains/Constructs
Dienhart MK and Stuart RA (2008) The Yeast Aac2 Protein Exists in Physical Association with the Cytochrome bc1-COX Supercomplex and the TIM23 Machinery. Mol Biol Cell 19(9):3934-43
       |COR1 |COX2 |PAM16 |QCR2 |QCR6 |QCR7 |TIM17 |TIM23 |TIM44 |TIM50
Genetic Interactions
Mutants/Phenotypes
Hlavata L, et al. (2008) Elevated Ras/protein kinase A activity in Saccharomyces cerevisiae reduces proliferation rate and lifespan by two different reactive oxygen species-dependent routes. Aging Cell 7(2):148-57
         |BCY1 |RAS2 |SOD1
Alias
Cross-species Expression
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Protein Sequence Features
Strains/Constructs
Iwahashi A, et al. (2008) Functionally important conserved length of C-terminal regions of yeast and bovine ADP/ATP carriers, identified by deletion mutants studies, and water accessibility of the amino acids at the C-terminal region of the yeast carrier. Mitochondrion 8(2):196-204

Non-Fungal Related Genes/Proteins
Protein Sequence Features
Strains/Constructs
Kihira Y (2008) Functional Interactions between Transmembrane Regions and Their C-Terminal Adjoining Loop in Mitochondrial ADP/ATP Carrier. Biol Pharm Bull 31(11):2001-6

Alias
Function/Process
Genetic Interactions
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Kucejova B, et al. (2008) Pleiotropic effects of the yeast Sal1 and Aac2 carriers on mitochondrial function via an activity distinct from adenine nucleotide transport. Mol Genet Genomics 280(1):25-39
       |AAC1 |GGC1 |MTG1 |SAL1 |SHY1
Alias
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Mroczek S and Kufel J (2008) Apoptotic signals induce specific degradation of ribosomal RNA in yeast. Nucleic Acids Res 36(9):2874-88
       |AAC1 |AAC3 |AHP1 |AIF1 |DOT5 |GPX1 |GPX2 |GRX1 |GRX2 |GSH1 |HTB1 |HTB2 |HYR1 |MCA1 |MORE
Genetic Interactions
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Smith CP and Thorsness PE (2008) The Molecular Basis for Relative Physiological Functionality of the ADP/ATP Carrier Isoforms in Saccharomyces cerevisiae. Genetics 179(3):1285-99
       |AAC1 |AAC3 |SAL1
Reviews
Stuart RA (2008) Supercomplex organization of the oxidative phosphorylation enzymes in yeast mitochondria. J Bioenerg Biomembr 40(5):411-7
       |ATP1 |ATP14 |ATP15 |ATP16 |ATP17 |ATP18 |ATP19 |ATP2 |ATP20 |ATP3 |ATP4 |ATP5 |ATP6 |ATP7 |MORE
Alias
Function/Process
Genetic Interactions
Strains/Constructs
Substrates/Ligands/Cofactors
Traba J, et al. (2008) Yeast mitochondria import ATP through the calcium-dependent ATP-Mg/Pi carrier Sal1p, and are ATP consumers during aerobic growth in glucose. Mol Microbiol 69(3):570-85
       |AAC1 |MRS2 |SAL1
Disease Gene Related
Function/Process
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Protein Sequence Features
Strains/Constructs
Wang X, et al. (2008) Dominant membrane uncoupling by mutant adenine nucleotide translocase in mitochondrial diseases. Hum Mol Genet 17(24):4036-44

Alias
Disease Gene Related
Function/Process
Genetic Interactions
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Wang X, et al. (2008) Reduced cytosolic protein synthesis suppresses mitochondrial degeneration. Nat Cell Biol 10(9):1090-7
     |ATP1 |COX2 |COX4 |CYT1 |FOB1 |GPR1 |HSP60 |PHB1 |PHB2 |REI1 |RPD3 |RPL31A |RPL6B |SCH9 |MORE
Alias
Cellular Location
Function/Process
Mutants/Phenotypes
Protein Processing/Modification/Regulation
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Bamber L, et al. (2007) The yeast mitochondrial ADP/ATP carrier functions as a monomer in mitochondrial membranes. Proc Natl Acad Sci U S A 104(26):10830-4

Function/Process
Strains/Constructs
Substrates/Ligands/Cofactors
Techniques and Reagents
Bamber L, et al. (2007) Yeast Mitochondrial ADP/ATP Carriers Are Monomeric in Detergents as Demonstrated by Differential Affinity Purification. J Mol Biol 371(2):388-95

Alias
Genetic Interactions
Protein-protein Interactions
Strains/Constructs
Buttner S, et al. (2007) Endonuclease g regulates budding yeast life and death. Mol Cell 25(2):233-46
       |AAC1 |AAC3 |AIF1 |HHF1 |HHF2 |HTB1 |HTB2 |KAP123 |MCA1 |NUC1 |POR1
Protein-protein Interactions
Davis AJ, et al. (2007) The Tim9p/10p and Tim8p/13p complexes bind to specific sites on Tim23p during mitochondrial protein import. Mol Biol Cell 18(2):475-86
     |TIM10 |TIM13 |TIM23 |TIM8 |TIM9
Alias
Non-Fungal Related Genes/Proteins
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Substrates/Ligands/Cofactors
Kihira Y, et al. (2007) Difference between Yeast and Bovine Mitochondrial ADP/ATP Carriers in Terms of Conformational Properties of the First Matrix Loop as Deduced by Use of Copper-o-phenanthroline. Biol Pharm Bull 30(5):885-90
       |AAC1
Protein-protein Interactions
Mick DU, et al. (2007) Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly. EMBO J 26(20):4347-58
       |COA1 |COR1 |COX1 |COX14 |COX2 |COX3 |COX4 |COX5A |CYT1 |MSS51 |QCR2 |QCR6 |RIP1 |SHY1
Function/Process
Mutants/Phenotypes
Strains/Constructs
Pereira C, et al. (2007) ADP/ATP carrier is required for mitochondrial outer membrane permeabilization and cytochrome c release in yeast apoptosis. Mol Microbiol 66(3):571-82
         |AAC1 |AAC3 |ATP2 |CPR3 |MCA1 |POR1
Protein Processing/Modification/Regulation
Protein Sequence Features
Reinders J, et al. (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
       |AAC1 |ACH1 |ACO1 |ADY2 |ALD4 |ATO2 |ATO3 |ATP1 |ATP15 |ATP16 |ATP2 |ATP20 |ATP4 |ATP5 |MORE
Function/Process
Strains/Constructs
Techniques and Reagents
Rey M, et al. (2007) The Saccharomyces cerevisiae ADP/ATP carrier-iso 1 cytochrome c fusion protein: One-step purification and functional analysis in vitro. Biochimie 89(9):1070-1079
       |CYC1
Regulation of
de Groot MJ, et al. (2007) Quantitative proteomics and transcriptomics of anaerobic and aerobic yeast cultures reveals post-transcriptional regulation of key cellular processes. Microbiology 153(Pt 11):3864-3878
         |ACO1 |ACO2 |ACS1 |ACS2 |ADE13 |ADE17 |ADE2 |ADE3 |ADE4 |ADE5,7 |ADE6 |ADH1 |ADH2 |ADH5 |MORE
Cellular Location
Protein-protein Interactions
Techniques and Reagents
Brandina I, et al. (2006) Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta 1757(9-10):1217-1228
       |ACO1 |ATP1 |ATP2 |CDC19 |CIT1 |CIT3 |COX2 |ENO1 |ENO2 |FBA1 |GPM1 |HXK1 |HXK2 |MDH1 |MORE
Evolution
Fungal Related Genes/Proteins
De Hertogh B, et al. (2006) Emergence of species-specific transporters during evolution of the hemiascomycete phylum. Genetics 172(2):771-81
       |AAC1 |AAC3 |ACS2 |ADP1 |ADY2 |AGC1 |AGP1 |AGP2 |AGP3 |ALP1 |ALR1 |ALR2 |ANT1 |AQR1 |MORE
Cross-species Expression
Fungal Related Genes/Proteins
Fontanesi F, et al. (2006) Heterologous complementation of the Klaac null mutation of Kluyveromyces lactis by the Saccharomyces cerevisiae AAC3 gene encoding the ADP/ATP carrier. FEMS Yeast Res 6(3):414-20
       |AAC1 |AAC3
Function/Process
Mutants/Phenotypes
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Iwahashi A, et al. (2006) The structure of the second cytosolic loop of the yeast mitochondrial ADP/ATP carrier AAC2 is dependent on the conformational state. Mitochondrion 6(5):245-51

Function/Process
Mutants/Phenotypes
Protein Sequence Features
Kihira Y, et al. (2006) Roles of adjoining Asp and Cys residues of first matrix-facing loop in transport activity of yeast and bovine mitochondrial ADP/ATP carriers. J Biochem 139(3):575-82

Alias
Cross-species Expression
Disease Gene Related
Non-Fungal Related Genes/Proteins
Strains/Constructs
Lodi T, et al. (2006) Mutation D104G in ANT1 gene: complementation study in Saccharomyces cerevisiae as a model system. Biochem Biophys Res Commun 341(3):810-5
           |AAC1
Regulation of
Transcription
Mojzita D and Hohmann S (2006) Pdc2 coordinates expression of the THI regulon in the yeast Saccharomyces cerevisiae. Mol Genet Genomics 276(2):147-61
         |ARO10 |FSH1 |INO1 |IPP1 |ITR1 |KGD2 |MDH1 |NRT1 |OLE1 |PDC1 |PDC2 |PDC5 |PET18 |PHO3 |MORE
Reviews
Palmieri F, et al. (2006) Identification of mitochondrial carriers in Saccharomyces cerevisiae by transport assay of reconstituted recombinant proteins. Biochim Biophys Acta 1757(9-10):1249-62
       |AAC1 |AAC3 |AGC1 |ANT1 |CAT2 |CRC1 |CTP1 |DIC1 |FLX1 |GGC1 |LEU5 |MIR1 |MRS3 |MRS4 |MORE
Cellular Location
Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
           |AAC1 |AAC3 |AAT1 |ABC1 |ABF2 |ACC1 |ACH1 |ACK1 |ACN9 |ACO1 |ACO2 |ACP1 |ACS1 |ADH3 |MORE
Fungal Related Genes/Proteins
Mutants/Phenotypes
Snoek IS and Steensma HY (2006) Why does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome. FEMS Yeast Res 6(3):393-403
       |ACP1 |ARB1 |ARG82 |ARH1 |ARV1 |ATM1 |BTS1 |BUR6 |CAB2 |CAX4 |CDC40 |CNM67 |CTF4 |DBP7 |MORE
Protein Processing/Modification/Regulation
Protein-protein Interactions
Techniques and Reagents
Tagwerker C, et al. (2006) A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking. Mol Cell Proteomics 5(4):737-48
         |AAC1 |AAC3 |ACB1 |ACC1 |ACS2 |ADE3 |ADE5,7 |ADE6 |ADH4 |ADO1 |AGP1 |AHA1 |AHP1 |ALA1 |MORE
Reviews
Arco AD and Satrustegui J (2005) New mitochondrial carriers: an overview. Cell Mol Life Sci 62(19-20):2204-27
       |AAC1 |AGC1 |ANT1 |CRC1 |CTP1 |DIC1 |FLX1 |GGC1 |LEU5 |MIR1 |MRS3 |MRS4 |MTM1 |OAC1 |MORE
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Cavero S, et al. (2005) The calcium-dependent ATP-Mg/Pi mitochondrial carrier is a target of glucose-induced calcium signalling in Saccharomyces cerevisiae. Biochem J 392(Pt 3):537-44
       |AAC1 |AAC3 |SAL1
Cellular Location
Function/Process
Mutants/Phenotypes
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Dahout-Gonzalez C, et al. (2005) Conformation-dependent swinging of the matrix loop m2 of the mitochondrial Saccharomyces cerevisiae ADP/ATP carrier. Biochemistry 44(49):16310-20

Function/Process
Strains/Constructs
Dassa EP, et al. (2005) Functional characterization and purification of a Saccharomyces cerevisiae ADP/ATP carrier-iso 1 cytochrome c fusion protein. Protein Expr Purif 40(2):358-69
         |CYC1
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Dzugasova V and Subik J (2005) Synthetic lethal interaction between the pel1 and op1 mutations in Saccharomyces cerevisiae. Folia Microbiol (Praha) 50(4):293-9
     |CRD1 |PGS1
Function/Process
Mutants/Phenotypes
Protein Physical Properties
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Kihira Y, et al. (2005) Cysteine labeling studies detect conformational changes in region 106-132 of the mitochondrial ADP/ATP carrier of Saccharomyces cerevisiae. Biochemistry 44(1):184-92

Cross-species Expression
Function/Process
Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Mentel M, et al. (2005) Triplicate genes for mitochondrial ADP/ATP carriers in the aerobic yeast Yarrowia lipolytica are regulated differentially in the absence of oxygen. Mol Genet Genomics 273(1):84-91

Cross-species Expression
Mutants/Phenotypes
Protein Sequence Features
Monne M, et al. (2005) Functional expression of eukaryotic membrane proteins in Lactococcus lactis. Protein Sci 14(12):3048-56
       |AAC1 |AAC3 |DIC1 |GGC1 |MIR1 |OAC1 |ODC1 |ODC2 |PET8
Disease Gene Related
Function/Process
Mutants/Phenotypes
Strains/Constructs
Palmieri L, et al. (2005) Complete loss-of-function of the heart/muscle-specific adenine nucleotide translocator is associated with mitochondrial myopathy and cardiomyopathy. Hum Mol Genet 14(20):3079-88

Function/Process
Strains/Constructs
Substrates/Ligands/Cofactors
Postis V, et al. (2005) Subunits of the yeast mitochondrial ADP/ATP carrier: cooperation within the dimer. Biochemistry 44(45):14732-40
       |MIR1
RNA Levels and Processing
Regulation of
Transcription
Puig S, et al. (2005) Coordinated remodeling of cellular metabolism during iron deficiency through targeted mRNA degradation. Cell 120(1):99-110
             |ACO1 |AFT1 |AFT2 |CCC1 |CCP1 |CIR2 |CIT1 |CIT3 |COQ6 |COR1 |COX4 |COX6 |COX8 |COX9 |MORE
Cellular Location
Protein Sequence Features
Vergnolle MA, et al. (2005) A cryptic matrix targeting signal of the yeast ADP/ATP carrier normally inserted by the TIM22 complex is recognized by the TIM23 machinery. Biochem J 385(Pt 1):173-80
       |TIM10 |TIM12 |TIM22 |TIM23
Function/Process
Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Chen XJ (2004) Sal1p, a calcium-dependent carrier protein that suppresses an essential cellular function associated With the Aac2 isoform of ADP/ATP translocase in Saccharomyces cerevisiae. Genetics 167(2):607-17
       |AAC1 |AAC3 |SAL1
Alias
Disease Gene Related
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Fontanesi F, et al. (2004) Mutations in AAC2, equivalent to human adPEO-associated ANT1 mutations, lead to defective oxidative phosphorylation in Saccharomyces cerevisiae and affect mitochondrial DNA stability. Hum Mol Genet 13(9):923-34

Reviews
Reichert AS and Neupert W (2004) Mitochondriomics or what makes us breathe. Trends Genet 20(11):555-62
       |ATM1 |FZO1 |HSP60 |ISC1 |MGM1 |OXA1 |POR1 |SSC1 |TIM22 |TIM23 |TIM8 |YFH1
Fungal Related Genes/Proteins
Zhang Z and Kishino H (2004) Genomic background predicts the fate of duplicated genes: evidence from the yeast genome. Genetics 166(4):1995-9
           |AAC1 |ALD4 |ALD5 |BDH1 |BDH2 |CAM1 |CCT4 |CCT6 |CDC19 |CLB3 |CLB4 |CYS3 |DCS1 |DCS2 |MORE
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Dunn CD and Jensen RE (2003) Suppression of a defect in mitochondrial protein import identifies cytosolic proteins required for viability of yeast cells lacking mitochondrial DNA. Genetics 165(1):35-45
     |ATP2 |CCT6 |COQ4 |CYT1 |ICY1 |PBP1 |SSB1 |TIM10 |TIM18 |TIM22 |TIM54 |TIP41 |TOM70 |YME1
Cellular Location
Mutants/Phenotypes
Protein Sequence Features
Protein-protein Interactions
Strains/Constructs
Dyall SD, et al. (2003) The dynamic dimerization of the yeast ADP/ATP carrier in the inner mitochondrial membrane is affected by conserved cysteine residues. J Biol Chem 278(29):26757-64
       |AAC1 |TIM10 |TIM22
Strains/Constructs
Techniques and Reagents
Kunji ER, et al. (2003) Lactococcus lactis as host for overproduction of functional membrane proteins. Biochim Biophys Acta 1610(1):97-108

Protein Physical Properties
Protein/Nucleic Acid Structure
Lorenz-Fonfria VA, et al. (2003) Structural and functional implications of the instability of the ADP/ATP transporter purified from mitochondria as revealed by FTIR spectroscopy. Biophys J 85(1):255-66

Cellular Location
Protein Sequence Features
Protein/Nucleic Acid Structure
Techniques and Reagents
Panneels V, et al. (2003) Choline head groups stabilize the matrix loop regions of the ATP/ADP carrier ScAAC2. Biochem Biophys Res Commun 300(1):65-74

Cellular Location
Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12
         |AAC1 |AAC3 |AAT1 |ABC1 |ABF2 |ACC1 |ACH1 |ACK1 |ACO1 |ACO2 |ACP1 |ACS1 |ADH3 |ADK2 |MORE
Function/Process
Mutants/Phenotypes
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Zeman I, et al. (2003) Four mutations in transmembrane domains of the mitochondrial ADP/ATP carrier increase resistance to bongkrekic acid. J Bioenerg Biomembr 35(3):243-56

Regulation of
Transcription
Zhang W, et al. (2003) Microarray analyses of the metabolic responses of Saccharomyces cerevisiae to organic solvent dimethyl sulfoxide. J Ind Microbiol Biotechnol 30(1):57-69
         |AAH1 |ABP140 |ACS2 |ADE1 |ADE12 |ADE17 |ADE4 |ADE8 |ADH2 |ADH4 |ADH5 |ADK1 |ADO1 |ALD3 |MORE
Function/Process
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Chen XJ (2002) Induction of an unregulated channel by mutations in adenine nucleotide translocase suggests an explanation for human ophthalmoplegia. Hum Mol Genet 11(16):1835-43

Cellular Location
Jensen RE and Dunn CD (2002) Protein import into and across the mitochondrial inner membrane: role of the TIM23 and TIM22 translocons. Biochim Biophys Acta 1592(1):25-34
         |TIM22 |TIM23 |TIM54
RNA Levels and Processing
Regulation of
Palkova Z, et al. (2002) Ammonia pulses and metabolic oscillations guide yeast colony development. Mol Biol Cell 13(11):3901-14
           |AAC3 |AAT1 |ACO1 |ACS1 |ADY2 |ALD4 |ALD5 |ATO2 |ATO3 |ATP15 |ATP2 |CAT2 |CHA1 |CIT3 |MORE
Cellular Location
Truscott KN, et al. (2002) Mitochondrial import of the ADP/ATP carrier: the essential TIM complex of the intermembrane space is required for precursor release from the TOM complex. Mol Cell Biol 22(22):7780-9
       |MGE1 |TIM10 |TIM12 |TIM13 |TIM17 |TIM18 |TIM22 |TIM23 |TIM44 |TIM54 |TIM8 |TIM9 |TOM20 |TOM22 |MORE
Alias
Cellular Location
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Substrates/Ligands/Cofactors
Techniques and Reagents
Hatanaka T, et al. (2001) Characterization of loops of the yeast mitochondrial ADP/ATP carrier facing the cytosol by site-directed mutagenesis. Biochem Biophys Res Commun 286(5):936-42

Alias
DNA/RNA Sequence Features
Function/Process
Non-Fungal Related Genes/Proteins
Protein Sequence Features
Strains/Constructs
Hatanaka T, et al. (2001) Significant effect of the N-terminal region of the mitochondrial ADP/ATP carrier on its efficient expression in yeast mitochondria. J Biol Chem 276(31):28881-8

Non-Fungal Related Genes/Proteins
Heimpel S, et al. (2001) Expression of the mitochondrial ADP/ATP carrier in Escherichia coli. Renaturation, reconstitution, and the effect of mutations on 10 positive residues. J Biol Chem 276(15):11499-506
       |AAC1
Alias
Cellular Location
DNA/RNA Sequence Features
Function/Process
Mutants/Phenotypes
Protein Physical Properties
Substrates/Ligands/Cofactors
Huang SG, et al. (2001) Chimers of two fused ADP/ATP carrier monomers indicate a single channel for ADP/ATP transport. Arch Biochem Biophys 394(1):67-75

Cellular Location
Protein-protein Interactions
Regulation of
Strains/Constructs
Wiedemann N, et al. (2001) The three modules of ADP/ATP carrier cooperate in receptor recruitment and translocation into mitochondria. EMBO J 20(5):951-60
       |AAC1 |AAC3 |TOM70
Reviews
Chen XJ and Clark-Walker GD (2000) The petite mutation in yeasts: 50 years on. Int Rev Cytol 194:197-238
     |PGS1 |YME1
Function/Process
Protein Sequence Features
Strains/Constructs
Substrates/Ligands/Cofactors
Techniques and Reagents
Dianoux AC, et al. (2000) Two distinct regions of the yeast mitochondrial ADP/ATP carrier are photolabeled by a new ADP analogue: 2-azido-3'-O-naphthoyl-[beta-32P]ADP. Identification of the binding segments by mass spectrometry. Biochemistry 39(37):11477-87

Function/Process
Non-Fungal Related Genes/Proteins
Other Features
Dyall SD, et al. (2000) Presence of a member of the mitochondrial carrier family in hydrogenosomes: conservation of membrane-targeting pathways between hydrogenosomes and mitochondria. Mol Cell Biol 20(7):2488-97
       |AAC1 |AAC3 |TIM10 |TIM9
Alias
Function/Process
Substrates/Ligands/Cofactors
Techniques and Reagents
Fiore C, et al. (2000) Purification of histidine-tagged mitochondrial ADP/ATP carrier: influence of the conformational states of the C-terminal region. Protein Expr Purif 19(1):57-65
       |AAC1 |AAC3
Mutants/Phenotypes
Other Features
Strains/Constructs
Harris MH, et al. (2000) Role of oxidative phosphorylation in Bax toxicity. Mol Cell Biol 20(10):3590-6
       |ATP4 |COX4 |COX7 |CYC3 |NDI1 |POR1 |QCR7 |SDH3
Function/Process
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Kissova I, et al. (2000) The cytotoxic action of Bax on yeast cells does not require mitochondrial ADP/ATP carrier but may be related to its import to the mitochondria. FEBS Lett 471(1):113-8
       |AAC1 |AAC3
Function/Process
Mutants/Phenotypes
Substrates/Ligands/Cofactors
Techniques and Reagents
Lunde CS and Kubo I (2000) Effect of polygodial on the mitochondrial ATPase of Saccharomyces cerevisiae. Antimicrob Agents Chemother 44(7):1943-53
       |PMA1
Function/Process
Protein Sequence Features
Protein-protein Interactions
Substrates/Ligands/Cofactors
Techniques and Reagents
Trezeguet V, et al. (2000) A covalent tandem dimer of the mitochondrial ADP/ATP carrier is functional in vivo. Biochim Biophys Acta 1457(1-2):81-93

Function/Process
Other Features
Chen XJ and Clark-Walker GD (1999) Alpha and beta subunits of F1-ATPase are required for survival of petite mutants in Saccharomyces cerevisiae. Mol Gen Genet 262(4-5):898-908
     |ATP1 |ATP15 |ATP16 |ATP2 |PGS1 |YME1
RNA Levels and Processing
Regulation of
Ferea TL, et al. (1999) Systematic changes in gene expression patterns following adaptive evolution in yeast. Proc Natl Acad Sci U S A 96(17):9721-6
           |ACO1 |ACS1 |ADE5,7 |ADH1 |ADH2 |ALD4 |ALD6 |ATP1 |ATP14 |ATP16 |ATP17 |ATP3 |ATP4 |ATP5 |MORE
Cellular Location
Function/Process
Protein-protein Interactions
Hatanaka T, et al. (1999) Functional expression of the tandem-repeated homodimer of the mitochondrial ADP/ATP carrier in Saccharomyces cerevisiae. Biochem Biophys Res Commun 262(3):726-30

Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Nebohacova M, et al. (1999) Isolation and expression of the gene encoding mitochondrial ADP/ATP carrier (AAC) from the pathogenic yeast Candida parapsilosis. Yeast 15(12):1237-42
       |AAC3
Protein Processing/Modification/Regulation
Protein-protein Interactions
Ryan MT, et al. (1999) Functional staging of ADP/ATP carrier translocation across the outer mitochondrial membrane. J Biol Chem 274(29):20619-27
       |AAC1 |AAC3 |SAM37 |TIM10 |TOM20 |TOM40 |TOM70
Alias
Fungal Related Genes/Proteins
Viola AM, et al. (1999) A Klaac null mutant of Kluyveromyces lactis is complemented by a single copy of the Saccharomyces cerevisiae AAC1 gene. Curr Genet 36(1-2):29-36
       |AAC1 |HAP2
Cellular Location
Protein Processing/Modification/Regulation
Kubrich M, et al. (1998) The import route of ADP/ATP carrier into mitochondria separates from the general import pathway of cleavable preproteins at the trans side of the outer membrane. J Biol Chem 273(26):16374-81
       |TOM22 |TOM7 |TOM70
Alias
Mutants/Phenotypes
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Nelson DR, et al. (1998) Highly conserved charge-pair networks in the mitochondrial carrier family. J Mol Biol 277(2):285-308

Function/Process
Fungal Related Genes/Proteins
Mutants/Phenotypes
Strains/Constructs
Joseph-Liauzun E, et al. (1997) The Mr 18,000 subunit of the peripheral-type benzodiazepine receptor exhibits both benzodiazepine and isoquinoline carboxamide binding sites in the absence of the voltage-dependent anion channel or of the adenine nucleotide carrier. J Biol Chem 272(44):28102-6
       |AAC1 |AAC3 |POR1
Alias
Function/Process
Mutants/Phenotypes
Protein Sequence Features
Protein/Nucleic Acid Structure
Strains/Constructs
Substrates/Ligands/Cofactors
Techniques and Reagents
Muller V, et al. (1997) Mutagenesis of some positive and negative residues occurring in repeat triad residues in the ADP/ATP carrier from yeast. Biochemistry 36(50):16008-18

Alias
Mutants/Phenotypes
Strains/Constructs
Polcic P, et al. (1997) Fatty acids induced uncoupling of Saccharomyces cerevisiae mitochondria requires an intact ADP/ATP carrier. FEBS Lett 412(1):207-10

Alias
Mutants/Phenotypes
Strains/Constructs
Roucou X, et al. (1997) Conditions allowing different states of ATP- and GDP-induced permeability in mitochondria from different strains of Saccharomyces cerevisiae. Biochim Biophys Acta 1324(1):120-32

Cellular Location
Function/Process
Fungal Related Genes/Proteins
Protein Physical Properties
Protein Sequence Features
Protein/Nucleic Acid Structure
el Moualij B, et al. (1997) Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae. Yeast 13(6):573-81
       |AAC1 |AAC3 |AGC1 |ANT1 |CRC1 |CTP1 |DIC1 |FLX1 |GGC1 |LEU5 |MIR1 |MRS3 |MRS4 |MTM1 |MORE
Alias
Cross-species Expression
Fungal Related Genes/Proteins
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Couzin N, et al. (1996) Cloning of the gene encoding the mitochondrial adenine nucleotide carrier of Schizosaccharomyces pombe by functional complementation in Saccharomyces cerevisiae. Gene 171(1):113-7
       |AAC1 |AAC3
Alias
Cellular Location
Function/Process
Fungal Related Genes/Proteins
Gavurnikova G, et al. (1996) Transcription of the AAC1 gene encoding an isoform of mitochondrial ADP/ATP carrier in Saccharomyces cerevisiae is regulated by oxygen in a heme-independent manner. Eur J Biochem 239(3):759-63
     |AAC1 |AAC3
Alias
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Substrates/Ligands/Cofactors
Le Saux A, et al. (1996) Conformational changes of the yeast mitochondrial adenosine diphosphate/adenosine triphosphate carrier studied through its intrinsic fluorescence. 1. Tryptophanyl residues of the carrier can be mutated without impairing protein activity. Biochemistry 35(50):16116-24

Alias
Function/Process
Fungal Related Genes/Proteins
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Protein/Nucleic Acid Structure
Strains/Constructs
Techniques and Reagents
Muller V, et al. (1996) Probing the role of positive residues in the ADP/ATP carrier from yeast. The effect of six arginine mutations of oxidative phosphorylation and AAC expression. Biochemistry 35(50):16132-43

Alias
Regulation of
Nebohacova M, et al. (1996) Oxygen- and carbon source-dependent transactivation effect of ABF1 on the expression of the AAC2 gene encoding mitochondrial ADP/ATP carrier. Folia Microbiol (Praha) 41(1):115-6
     |ABF1 |CHA4
Alias
Protein Physical Properties
Protein Sequence Features
Protein/Nucleic Acid Structure
Substrates/Ligands/Cofactors
Roux P, et al. (1996) Conformational changes of the yeast mitochondrial adenosine diphosphate/adenosine triphosphate carrier studied through its intrinsic fluorescence. 2. Assignment of tryptophanyl residues of the carrier to the responses to specific ligands. Biochemistry 35(50):16125-31

Mapping
Song JM, et al. (1996) Organization and characterization of the two yeast ribosomal protein YL19 genes. Curr Genet 30(4):273-8
       |RPL19A |RPL19B |YBL028C |YBL029W
Alias
DNA/RNA Sequence Features
Fungal Related Genes/Proteins
Mutants/Phenotypes
Protein Processing/Modification/Regulation
RNA Levels and Processing
Regulation of
Strains/Constructs
Betina S, et al. (1995) Expression of the AAC2 gene encoding the major mitochondrial ADP/ATP carrier in Saccharomyces cerevisiae is controlled at the transcriptional level by oxygen, heme and HAP2 factor. Eur J Biochem 229(3):651-7
     |AAC1 |AAC3 |ABF1 |HAP2 |HAP3
Cellular Location
Protein Processing/Modification/Regulation
Haucke V, et al. (1995) The yeast mitochondrial protein import receptor Mas20p binds precursor proteins through electrostatic interaction with the positively charged presequence. J Biol Chem 270(10):5565-70
       |HSP10 |HSP60 |RIP1 |SAM37 |TOM20 |TOM22 |TOM70
Alias
Cross-species Expression
Fungal Related Genes/Proteins
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Viola AM, et al. (1995) A kluyveromyces lactis gene homologue to AAC2 complements the Saccaromyces cerevisiae op1 mutation. Curr Genet 27(3):229-33
     |AAC1
Alias
Function/Process
Mutants/Phenotypes
Protein Physical Properties
Protein/Nucleic Acid Structure
Strains/Constructs
Techniques and Reagents
Hoffmann B, et al. (1994) The reconstituted ADP/ATP carrier activity has an absolute requirement for cardiolipin as shown in cysteine mutants. J Biol Chem 269(3):1940-4
     |AAC1
Mutants/Phenotypes
Strains/Constructs
Kule C, et al. (1994) Doxorubicin, daunorubicin, and mitoxantrone cytotoxicity in yeast. Mol Pharmacol 46(6):1234-40

Alias
Mapping
Van Dyck L, et al. (1994) Analysis of a 17.4 kb DNA segment of yeast chromosome II encompassing the ribosomal protein L19 as well as proteins with homologies to components of the hnRNP and snRNP complexes and to the human proliferation-associated p120 antigen. Yeast 10(12):1663-73
     |HEK2 |MCM2 |SHE1
Alias
Genetic Interactions
Mutants/Phenotypes
Protein Physical Properties
Strains/Constructs
Substrates/Ligands/Cofactors
Techniques and Reagents
Brandolin G, et al. (1993) Biochemical characterisation of the isolated Anc2 adenine nucleotide carrier from Saccharomyces cerevisiae mitochondria. Biochem Biophys Res Commun 192(1):143-50
     |AAC1 |AAC3
Mutants/Phenotypes
Non-Fungal Related Genes/Proteins
Strains/Constructs
Brandolin G, et al. (1993) Chemical, immunological, enzymatic, and genetic approaches to studying the arrangement of the peptide chain of the ADP/ATP carrier in the mitochondrial membrane. J Bioenerg Biomembr 25(5):459-72

Mutants/Phenotypes
Strains/Constructs
Meunier B, et al. (1993) Genetic screening in Saccharomyces cerevisiae for large numbers of mitochondrial point mutations which affect structure and function of catalytic subunits of cytochrome-c oxidase. Eur J Biochem 213(1):129-35
     |COX1 |COX2 |COX3
Alias
DNA/RNA Sequence Features
Genetic Interactions
Mutants/Phenotypes
Protein/Nucleic Acid Structure
Strains/Constructs
Nelson DR and Douglas MG (1993) Function-based mapping of the yeast mitochondrial ADP/ATP translocator by selection for second site revertants. J Mol Biol 230(4):1171-82
     |AAC1
Alias
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Nelson DR, et al. (1993) Site-directed mutagenesis of the yeast mitochondrial ADP/ATP translocator. Six arginines and one lysine are essential. J Mol Biol 230(4):1159-70

Alias
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Drgon T, et al. (1992) Yeast ADP/ATP carrier (AAC) proteins exhibit similar enzymatic properties but their deletion produces different phenotypes. FEBS Lett 304(2-3):277-80
     |AAC1 |AAC3
Alias
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Drgon T, et al. (1991) ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae. FEBS Lett 289(2):159-62
     |AAC1 |AAC3
Mapping
Preston RA, et al. (1991) Map positions of pet9, pep1 and pdr4 with respect to CEN2. Yeast 7(8):857-8
     |CEN2 |PEP1
Alias
Function/Process
Genetic Interactions
Mutants/Phenotypes
Protein Physical Properties
Strains/Constructs
Techniques and Reagents
Gawaz M, et al. (1990) Structure-function studies of adenine nucleotide transport in mitochondria. II. Biochemical analysis of distinct AAC1 and AAC2 proteins in yeast. J Biol Chem 265(24):14202-8
     |AAC1
Alias
Function/Process
Fungal Related Genes/Proteins
Genetic Interactions
Mutants/Phenotypes
Protein Sequence Features
RNA Levels and Processing
Regulation of
Strains/Constructs
Kolarov J, et al. (1990) A third ADP/ATP translocator gene in yeast. J Biol Chem 265(21):12711-6
     |AAC1 |AAC3
Alias
Genetic Interactions
Mutants/Phenotypes
Protein Sequence Features
Strains/Constructs
Lawson JE, et al. (1990) Structure-function studies of adenine nucleotide transport in mitochondria. I. Construction and genetic analysis of yeast mutants encoding the ADP/ATP carrier protein of mitochondria. J Biol Chem 265(24):14195-201
     |AAC1
Alias
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Hapala L (1989) Growth defects in intramitochondrial energy depleted cells: role of mitochondrial biogenesis. Biochem Biophys Res Commun 159(2):612-7

Alias
Other Features
Substrates/Ligands/Cofactors
Techniques and Reagents
Knirsch M, et al. (1989) The isolation and reconstitution of the ADP/ATP carrier from wild-type Saccharomyces cerevisiae. Identification of primarily one type (AAC-2). FEBS Lett 244(2):427-32

Alias
Function/Process
Protein Sequence Features
Substrates/Ligands/Cofactors
Mayinger P, et al. (1989) The ADP/ATP carrier from yeast (AAC-2) is uniquely suited for the assignment of the binding center by photoaffinity labeling. FEBS Lett 244(2):421-6

Alias
DNA/RNA Sequence Features
Fungal Related Genes/Proteins
Genetic Interactions
Non-Fungal Related Genes/Proteins
Protein Sequence Features
Strains/Constructs
Lawson JE and Douglas MG (1988) Separate genes encode functionally equivalent ADP/ATP carrier proteins in Saccharomyces cerevisiae. Isolation and analysis of AAC2. J Biol Chem 263(29):14812-8
     |AAC1
Cellular Location
Protein Sequence Features
Smagula C and Douglas MG (1988) Mitochondrial import of the ADP/ATP carrier protein in Saccharomyces cerevisiae. Sequences required for receptor binding and membrane translocation. J Biol Chem 263(14):6783-90

Cellular Location
DNA/RNA Sequence Features
Fungal Related Genes/Proteins
Non-Fungal Related Genes/Proteins
Protein Physical Properties
Protein Sequence Features
Adrian GS, et al. (1986) Sequences required for delivery and localization of the ADP/ATP translocator to the mitochondrial inner membrane. Mol Cell Biol 6(2):626-34

Mapping
Subik J, et al. (1986) Genetic mapping of nuclear mucidin resistance mutations in Saccharomyces cerevisiae. A new pdr locus on chromosome II. Curr Genet 10(9):665-70
     |LYS2 |PDR3
Alias
Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Techniques and Reagents
McEwen JE, et al. (1985) Rapid method for isolation and screening of cytochrome c oxidase-deficient mutants of Saccharomyces cerevisiae. J Bacteriol 161(3):831-5
     |COX6
Alias
Cellular Location
Genetic Interactions
Mutants/Phenotypes
Protein Physical Properties
Strains/Constructs
Techniques and Reagents
O'Malley K, et al. (1982) Selection of the nuclear gene for the mitochondrial adenine nucleotide translocator by genetic complementation of the op1 mutation in yeast. J Biol Chem 257(4):2097-103

Genetic Interactions
Mutants/Phenotypes
Strains/Constructs
Sigler K, et al. (1981) Factors governing substrate-induced generation and extrusion of protons in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 643(3):572-82

Alias
Mutants/Phenotypes
Strains/Constructs
Horak J, et al. (1978) Stimulation of amino acid transport in Saccharomyces cerevisiae by metabolic inhibitors. Folia Microbiol (Praha) 23(4):286-91
     |AGP1 |AGP3 |ALP1 |BAP2 |BAP3 |CAN1 |DIP5 |GNP1 |PMA1
Alias
Mutants/Phenotypes
Strains/Constructs
Lauquin G, et al. (1976) Effect of genetic and physiological manipulations onthe kinetic and binding parameters of the adenine nucleotide translocator in Saccharomyces cervisiae and Candida utilis. Biochimie 58(10):1213-20

Alias
Mutants/Phenotypes
Strains/Constructs
Ulaszewski S and Lachowicz TM (1975) Specificity and origin of the Mod2- mutation which restores respiratory sufficient phenotype in some pet mutants in Saccharomyces cerevisiae. Acta Microbiol Pol [A] 7(2):77-85

Alias
Function/Process
Mutants/Phenotypes
Strains/Constructs
Beck JC, et al. (1968) Genetic modification of energy-conserving systems in yeast mitochondria. Proc Natl Acad Sci U S A 60(1):186-93

Function/Process
Mutants/Phenotypes
Strains/Constructs
Kovacova V, et al. (1968) Oxidative phosphorylatiion in yeast. IV. Combination of a nuclear mutation affecting oxidative phosphorylation with cytoplasmic mutation to respiratory deficiency. Biochim Biophys Acta 162(2):157-63

Alias
Function/Process
Mutants/Phenotypes
Strains/Constructs
Kovac L, et al. (1967) Biochemical genetics of oxidative phosphorylation. Science 158(808):1564-7

Return to SGD

SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.