Bergkessel M and Guthrie C (2013) Chemical transformation of yeast. Methods Enzymol 529():311-20
Abstract: Transformation of chemically competent yeast cells is a method for introducing exogenous DNA into living cells. Typically, the DNA is either a plasmid carrying an autonomous replication sequence that allows for propagation or a linear piece of DNA to be integrated into the genome. The DNA usually also carries a marker that allows for selection of successfully transformed cells by plating on the appropriate selective media.
|Status: Published||Type: Journal Article||PubMed ID: 24011057|