Sousa CA, et al. (2013) Saccharomyces cerevisiae Mutants Affected in Vacuole Assembly or Vacuolar H-ATPase are Hypersensitive to Lead (Pb) Toxicity. Curr Microbiol ()
Abstract: Lead is an important environmental pollutant. The role of vacuole, in Pb detoxification, was studied using a vacuolar protein sorting mutant strain (vps16Delta), belonging to class C mutants. Cells disrupted in VPS16 gene, did not display a detectable vacuolar-like structure. Based on the loss of cell proliferation capacity, it was found that cells from vps16Delta mutant exhibited a hypersensitivity to Pb-induced toxicity, compared to wild type (WT) strain. The function of vacuolar H+-ATPase (V-ATPase), in Pb detoxification, was evaluated using mutants with structurally normal vacuoles but defective in subunits of catalytic (vma1Delta or vma2Delta) or membrane domain (vph1Delta or vma3Delta) of V-ATPase. All mutants tested, lacking a functional V-ATPase, displayed an increased susceptibility to Pb, comparatively to cells from WT strain. Modification of vacuolar morphology, in Pb-exposed cells, was visualized using a Vma2p-GFP strain. The treatment of yeast cells with Pb originated the fusion of the medium size vacuolar lobes into one enlarged vacuole. In conclusion, it was found that vacuole plays an important role in the detoxification of Pb in Saccharomyces cerevisiae; in addition, a functional V-ATPase was required for Pb compartmentalization.
|Status: Epub ahead of print||Type: Journal Article||PubMed ID: 24013611|
Topics addressed in this paper
Number of different genes curated to this paper: 5
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