Ibanez AJ, et al. (2013) Mass spectrometry-based metabolomics of single yeast cells. Proc Natl Acad Sci U S A 110(22):8790-4
Abstract: Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-d-glucose) and genetically (?PFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.
|Status: Published||Type: Journal Article||PubMed ID: 23671112|
Topics addressed in this paper
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|Topics||Topics not linked to Genes|