Chang JS and Winston F (2013) Cell-Cycle Perturbations Suppress the Slow-Growth Defect of spt10Delta Mutants in Saccharomyces cerevisiae. G3 (Bethesda) 3(3):573-83
Abstract: Spt10 is a putative acetyltransferase of that directly activates the transcription of histone genes. Deletion of causes a severe slow growth phenotype, showing that Spt10 is critical for normal cell division. To gain insight into the function of Spt10, we identified mutations that impair or improve the growth of null mutants. Mutations that cause lethality in combination with include particular components of the SAGA complex as well as and . Partial suppressors of the growth defect include mutations that perturb cell-cycle progression through the G1/S transition, S phase, and G2/M. Consistent with these results, slowing of cell-cycle progression by treatment with hydroxyurea or growth on medium containing glycerol as the carbon source also partially suppresses the slow-growth defect. In addition, mutations that impair the Lsm1-7-Pat1 complex, which regulates decapping of polyadenylated mRNAs, also partially suppress the growth defect. Interestingly, suppression of the growth defect is not accompanied by a restoration of normal histone mRNA levels. These findings suggest that Spt10 has multiple roles during cell division.
|Status: Published||Type: Journal Article | Research Support, N.I.H., Extramural||PubMed ID: 23450643|
Topics addressed in this paper
Number of different genes curated to this paper: 16
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