SGD Paper Help


Page Contents:
Huang J, et al.  (2012) Lis1 Acts as a "Clutch" between the ATPase and Microtubule-Binding Domains of the Dynein Motor. Cell 150(5):975-86

Abstract: The lissencephaly protein Lis1 has been reported to regulate the mechanical behavior of cytoplasmic dynein, the primary minus-end-directed microtubule motor. However, the regulatory mechanism remains poorly understood. Here, we address this issue using purified proteins from Saccharomyces cerevisiae and a combination of techniques, including single-molecule imaging and single-particle electron microscopy. We show that rather than binding to the main ATPase site within dynein's AAA+ ring or its microtubule-binding stalk directly, Lis1 engages the interface between these elements. Lis1 causes individual dynein motors to remain attached to microtubules for extended periods, even during cycles of ATP hydrolysis that would canonically induce detachment. Thus, Lis1 operates like a "clutch" that prevents dynein's ATPase domain from transmitting a detachment signal to its track-binding domain. We discuss how these findings provide a conserved mechanism for dynein functions in living cells that require prolonged microtubule attachments.CI - Copyright (c) 2012 Elsevier Inc. All rights reserved.

Status: Published Type: Journal Article PubMed ID: 22939623

Topics addressed in this paper

Number of different genes curated to this paper: 5

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
DYN1 NDL1 NIP100 PAC1 PAC11
Additional Literature blue ball blue ball
Function/Process blue ball blue ball
Fungal Related Genes/Proteins blue ball
Mutants/Phenotypes blue ball blue ball blue ball
Non-Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball
Protein Sequence Features blue ball blue ball
Protein-protein Interactions blue ball blue ball
Protein/Nucleic Acid Structure blue ball blue ball
Strains/Constructs blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball
Techniques and Reagents blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: