SGD Paper Help


Page Contents:
Lee JS, et al.  (2012) Codependency of H2B monoubiquitination and nucleosome reassembly on Chd1. Genes Dev 26(9):914-9

Abstract: Monoubiquitination of histone H2B on Lys 123 (H2BK123ub) is a transient histone modification considered to be essential for establishing H3K4 and H3K79 trimethylation by Set1/COMPASS and Dot1, respectively. Here, we identified Chd1 as a factor that is required for the maintenance of high levels of H2B monoubiquitination, but not for H3K4 and H3K79 trimethylation. Loss of Chd1 results in a substantial loss of H2BK123ub levels with little to no effect on the genome-wide pattern of H3K4 and H3K79 trimethylation. Our data show that nucleosomal occupancy is reduced in gene bodies in both chd1Delta and, as has been shown, K123A mutant backgrounds. We also demonstrated that Chd1's function in maintaining H2BK123ub levels is conserved from yeast to humans. Our study provides evidence that only small levels of H2BK123ub are necessary for full levels of H3K4 and H3K79 trimethylation in vivo and points to a possible role for Chd1 in positively regulating gene expression through promoting nucleosome reassembly coupled with H2B monoubiquitination.

Status: Published Type: Journal Article PubMed ID: 22549955

Topics addressed in this paper

Number of different genes curated to this paper: 8

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Topics not linked to Genes Genes linked to topics
ARN2 BRE1 CHD1 HHT1 HHT2 HOM3 HTB1 HTB2
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball
Genomic co-immunoprecipitation study yg ball
Genomic expression study yg ball
Mutants/Phenotypes blue ball blue ball
Non-Fungal Related Genes/Proteins blue ball blue ball
Omics yg ball
Primary Literature blue ball
Protein Processing/Modification/Regulation blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball
Regulatory Role blue ball
Strains/Constructs blue ball blue ball blue ball blue ball
Transcription blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: