SGD Paper Help


Page Contents:
Zunder RM, et al.  (2012) Two surfaces on the histone chaperone Rtt106 mediate histone binding, replication, and silencing. Proc Natl Acad Sci U S A 109(3):E144-53

Abstract: The histone chaperone Rtt106 binds histone H3 acetylated at lysine 56 (H3K56ac) and facilitates nucleosome assembly during several molecular processes. Both the structural basis of this modification-specific recognition and how this recognition informs Rtt106 function are presently unclear. Guided by our crystal structure of Rtt106, we identified two regions on its double-pleckstrin homology domain architecture that mediated histone binding. When histone binding was compromised, Rtt106 localized properly to chromatin but failed to deliver H3K56ac, leading to replication and silencing defects. By mutating analogous regions in the structurally homologous chromatin-reorganizer Pob3, we revealed a conserved histone-binding function for a basic patch found on both proteins. In contrast, a loop connecting two beta-strands was required for histone binding by Rtt106 but was dispensable for Pob3 function. Unlike Rtt106, Pob3 histone binding was modification-independent, implicating the loop of Rtt106 in H3K56ac-specific recognition in vivo. Our studies described the structural origins of Rtt106 function, identified a conserved histone-binding surface, and defined a critical role for Rtt106:H3K56ac-binding specificity in silencing and replication-coupled nucleosome turnover.

Status: Published Type: Journal Article | Research Support, N.I.H., Extramural | Research Support, U.S. Gov't, Non-P.H.S. PubMed ID: 22198837

Topics addressed in this paper

Number of different genes curated to this paper: 5

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Topics not linked to Genes Genes linked to topics
HHT1 HHT2 POB3 RLF2 RTT106
Additional Literature blue ball blue ball blue ball
Cellular Location blue ball
Fungal Related Genes/Proteins blue ball blue ball
Genetic Interactions blue ball blue ball
Genomic co-immunoprecipitation study blue ball
Mutants/Phenotypes blue ball blue ball
Omics yg ball
Primary Literature blue ball blue ball
Protein Processing/Modification/Regulation blue ball blue ball
Protein Sequence Features blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball
Protein/Nucleic Acid Structure blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: