Dai J and Boeke JD (2012) Strain construction and screening methods for a yeast histone h3/h4 mutant library. Methods Mol Biol 833():1-14
Abstract: A mutant library consisting of hundreds of designed point and deletion mutants in the genes encoding Saccharomyces cerevisiae histones H3 and H4 is described. Incorporation of this library into a suitably engineered yeast strain (e.g., bearing a reporter of interest), and the validation of individual library members is described in detail.
|Status: Published||Type: Journal Article||PubMed ID: 22183584|
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