Chinen T, et al. (2011) Construction of multidrug-sensitive yeast with high sporulation efficiency. Biosci Biotechnol Biochem 75(8):1588-93
Abstract: Budding yeast is often used in chemical genetics for screening, target identification, and compound verification, but its high-level drug resistance has made the analysis of compounds difficult. Here we report the construction of 12gene?0HSR, a strain that lacks eight efflux pumps located on the plasma membrane and four transcription factors involved in expression of efflux pumps, and contains the RME1(ins-308A) mutation. This strain retained sufficient transformation, mating, and sporulation efficiency for genetic analysis in addition to hypersensitivity against several compounds. 12gene?0HSR is a useful tool for chemical biology, not only in chemical screening but in target identification and verification of bioactive compounds.
|Status: Published||Type: Journal Article||PubMed ID: 21821930|
Topics addressed in this paper
Number of different genes curated to this paper: 16
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|Topics||Genes (#1 - 10 )|
|Topics||Genes (#11 - 16 )|