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Schwer B, et al.  (2011) Composition of yeast snRNPs and snoRNPs in the absence of trimethylguanosine caps reveals nuclear cap binding protein as a gained U1 component implicated in the cold-sensitivity of tgs1? cells. Nucleic Acids Res 39(15):6715-28

Abstract: Small nuclear and nucleolar RNAs that program pre-mRNA splicing and rRNA processing have a signature 5'-trimethylguanosine (TMG) cap. Whereas the mechanism of TMG synthesis by Tgs1 methyltransferase has been elucidated, we know little about whether or how RNP biogenesis, structure and function are perturbed when TMG caps are missing. Here, we analyzed RNPs isolated by tandem-affinity purification from TGS1 and tgs1? yeast strains. The protein and U-RNA contents of total SmB-containing RNPs were similar. Finer analysis revealed stoichiometric association of the nuclear cap-binding protein (CBP) subunits Sto1 and Cbc2 with otherwise intact Mud1- and Nam8-containing U1 snRNPs from tgs1? cells. CBP was not comparably enriched in Lea1-containing U2 snRNPs from tgs1? cells. Moreover, CBP was not associated with mature Nop58-containing C/D snoRNPs or mature Cbf5- and Gar1-containing H/ACA snoRNPs from tgs1? cells. The protein composition and association of C/D snoRNPs with the small subunit (SSU) processosome were not grossly affected by absence of TMG caps, nor was the composition of H/ACA snoRNPs. The cold-sensitive (cs) growth defect of tgs1? yeast cells could be suppressed by mutating the cap-binding pocket of Cbc2, suggesting that ectopic CBP binding to the exposed U1 m(7)G cap in tgs1? cells (not lack of TMG caps per se) underlies the cs phenotype.

Status: Published Type: Journal Article PubMed ID: 21558325

Topics addressed in this paper

Number of different genes curated to this paper: 102

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Topics Genes linked to topics (#1 - 10 )
AAR2 BRE5 BRR1 BRR2 BUD13 CBC2 CBF5 CDC40 CEF1 CLF1
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Topics Genes linked to topics (#11 - 20 )
CUS1 CWC15 CWC2 CWC21 CWC22 CWC23 CWC25 CWC27 DED1 DIB1
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Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#21 - 30 )
EBS1 ECM2 HSH155 HSH49 ISY1 KAP95 LEA1 LHP1 LIN1 LSM12
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Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
LSM2 LSM3 LSM4 LSM5 LSM6 LSM7 LSM8 LSR1 LUC7 MSL1
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Topics Genes linked to topics (#41 - 50 )
MUD1 NAM8 NOP58 NTC20 NTR2 PAB1 PBP1 PBP4 PRP19 PRP21
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Topics Genes linked to topics (#51 - 60 )
PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46
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Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#61 - 70 )
PRP6 PRP8 PRP9 RSE1 SMB1 SMD1 SMD2 SMD3 SME1 SMX2
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Topics Genes linked to topics (#71 - 80 )
SMX3 SNP1 SNR11 SNR14 SNR19 SNR31 SNR33 SNR35 SNR42 SNR6
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Topics Genes linked to topics (#81 - 90 )
SNR7-L SNR7-S SNR8 SNT309 SNU114 SNU13 SNU23 SNU56 SNU66 SNU71
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Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Regulation of blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#91 - 100 )
SPP381 SPP382 SRP1 STO1 SYF1 SYF2 TGS1 UBP3 YHC1 YJU2
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Genetic Interactions blue ball
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Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#101 - 102 )
YRA1 YSF3
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