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Forsmark A, et al.  (2011) Quantitative proteomics of yeast post-Golgi vesicles reveals a discriminating role for Sro7p in protein secretion. Traffic 12(6):740-53

Abstract: We here report the first comparative proteomics of purified yeast post-Golgi vesicles (PGVs). Vesicle samples isolated from PGV-accumulating sec6-4 mutants were treated with isobaric tags (iTRAQ) for subsequent quantitative tandem mass spectrometric analysis of protein content. After background subtraction, a total of 66 vesicle-associated proteins were identified, including known or assumed vesicle residents as well as a fraction not previously known to be PGV associated. Vesicles isolated from cells lacking the polarity protein Sro7p contained essentially the same catalogue of proteins but showed a reduced content of a subset of cargo proteins, in agreement with a previously shown selective role for Sro7p in cargo sorting.

Status: Published Type: Journal Article PubMed ID: 21477180

Topics addressed in this paper

Number of different genes curated to this paper: 58

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
AGP1 ATR1 BGL2 CDC42 CRH1 CWP1 DNF1 ECM33 EHT1 ENA1
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Topics Genes linked to topics (#11 - 20 )
ERG6 FAA1 FKS1 FKS3 GAS1 GAS5 GCN1 GNP1 GRX2 HXT5
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Topics Genes linked to topics (#21 - 30 )
HXT7 KIC1 KRE2 KTR1 KTR3 MRH1 MSC1 PDR5 PHM7 PIR1
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Topics Genes linked to topics (#31 - 40 )
PMA1 PNS1 PRC1 PST1 PTR2 RHO1 RTN1 SAC1 SEC4 SLK19
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Topics Genes linked to topics (#41 - 50 )
SNC2 SNQ2 SPF1 SRO7 SSS1 STE24 STL1 TSC13 VMA13 VPH1
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Topics Genes linked to topics (#51 - 58 )
VPS21 YGP1 YJL171C YNL194C YPT1 YPT31 YPT7 YRO2
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