Will CL and Luhrmann R (2010) Spliceosome Structure and Function.LID - cshperspect.a003707v1 [pii]LID - 10.1101/cshperspect.a003707 [doi] Cold Spring Harb Perspect Biol ()
Abstract: Pre-mRNA splicing is catalyzed by the spliceosome, a multimegadalton ribonucleoprotein (RNP) complex comprised of five snRNPs and numerous proteins. Intricate RNA-RNA and RNP networks, which serve to align the reactive groups of the pre-mRNA for catalysis, are formed and repeatedly rearranged during spliceosome assembly and catalysis. Both the conformation and composition of the spliceosome are highly dynamic, affording the splicing machinery its accuracy and flexibility, and these remarkable dynamics are largely conserved between yeast and metazoans. Because of its dynamic and complex nature, obtaining structural information about the spliceosome represents a major challenge. Electron microscopy has revealed the general morphology of several spliceosomal complexes and their snRNP subunits, and also the spatial arrangement of some of their components. X-ray and NMR studies have provided high resolution structure information about spliceosomal proteins alone or complexed with one or more binding partners. The extensive interplay of RNA and proteins in aligning the pre-mRNA's reactive groups, and the presence of both RNA and protein at the core of the splicing machinery, suggest that the spliceosome is an RNP enzyme. However, elucidation of the precise nature of the spliceosome's active site, awaits the generation of a high-resolution structure of its RNP core.
Status: Epub ahead of print
Type: Journal Article
PubMed ID: 21441581
Topics addressed in this paper
Number of different genes curated to this paper: 86
Jump to Summary Chart for:
To find other papers on a gene and topic, click on the colored ball in the appropriate box.
displays other papers with information about that topic for that gene.
displays other papers in SGD that are associated with that topic. The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
To go to the Locus page for a gene, click on the gene name.