Li Z, et al. (2011) Systematic exploration of essential yeast gene function with temperature-sensitive mutants. Nat Biotechnol 29(4):361-7
Abstract: Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 ( approximately 45%) of the 1,101 essential yeast genes, with approximately 30% of the genes represented by multiple alleles. All of the alleles are integrated into their native genomic locus in the S288C common reference strain and are linked to a kanMX selectable marker, allowing further genetic manipulation by synthetic genetic array (SGA)-based, high-throughput methods. We show two such manipulations: barcoding of 440 strains, which enables chemical-genetic suppression analysis, and the construction of arrays of strains carrying different fluorescent markers of subcellular structure, which enables quantitative analysis of phenotypes using high-content screening. Quantitative analysis of a GFP-tubulin marker identified roles for cohesin and condensin genes in spindle disassembly. This mutant collection should facilitate a wide range of systematic studies aimed at understanding the functions of essential genes.
|Status: Published||Type: Journal Article||PubMed ID: 21441928|
Topics addressed in this paper
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|Topics||Topics not linked to Genes||Genes linked to topics (#1 - 10 )|
|Large-scale genetic interaction|
|Large-scale phenotype analysis|
|Topics||Genes linked to topics (#11 - 20 )|
|Topics||Genes linked to topics (#21 - 25 )|