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Vaudano E, et al.  (2011) Identification of reference genes suitable for normalization of RT-qPCR expression data in Saccharomyces cerevisiae during alcoholic fermentation. Biotechnol Lett 33(8):1593-9

Abstract: Expression data from RT-qPCR (reverse transcription quantitative PCR) needs to be normalized to account for experimental variability among samples caused by differential yields of the transcripts in RNA extraction or in the reverse transcription. The most common method is to normalize against one or more reference genes (RG). We have selected RGs suitable for normalization of RT-qPCR raw data in Saccharomyces cerevisiae during alcoholic fermentation. The RGs were evaluated by three different statistical methods. The suitability of the selected RG sets was compared with ACT1, a commonly used non-validated single RG, by normalizing the expression of two target genes. Expression profiles of the target genes revealed the risk of misleading interpretation of expression data due to an unreliable RG.

Status: Published Type: Journal Article PubMed ID: 21452013

Topics addressed in this paper

Number of different genes curated to this paper: 13

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Topics Genes linked to topics (#1 - 10 )
ACT1 ADH1 GPD2 LSC2 PGK1 QCR9 RDN18-1 RDN18-2 TAF10 TDH2
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Topics Genes linked to topics (#11 - 13 )
TFC1 TUB2 UBC6
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