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Kang JJ, et al.  (2000) Transcript quantitation in total yeast cellular RNA using kinetic PCR. Nucleic Acids Res 28(2):e2

Abstract: Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and SAGE technologies for certain applications. The reproducibility, sensitivity and accuracy of the kRT-PCR were assessed for yeast transcripts previously quantitated by a variety of methods including SAGE analysis. Changes in transcript levels between different genetic or physiological cell states were reproducibly quantitated with an accuracy of +/-20%. The assay was sufficiently sensitive to quantitate yeast transcripts over a range of more than five orders of magnitude, including low abundance transcripts encoding cell cycle and transcriptional regulators.

Status: Published Type: Journal Article PubMed ID: 10606670

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ABF1 ACT1 ADH1 ADH2 CDC19 CLB1 CLB2 CYC1 ENO1 ENO2
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FBA1 FBP1 GCR1 GLK1 GPD1 GPD2 GPM1 GUT1 HXK1 HXK2
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PCK1 PDC1 PDC5 PFK1 PFK2 PGI1 PGK1 TDH1 TDH2 TDH3
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