Lam YT, et al. (2011) Changes in reactive oxygen species begin early during replicative aging of Saccharomyces cerevisiae cells. Free Radic Biol Med 50(8):963-70
Abstract: Increased reactive oxygen species (ROS) are a feature of aging cells, but little is known about when ROS generation begins as cells age. Here we show how ROS change in Saccharomyces cerevisiae cells throughout their early replicative lifespan using the fluorescent ROS indicator, dihydroethidium (DHE), which has some specificity for the superoxide anion. Cells in a particular age range were heterogeneous with respect to their ROS burden. Surprisingly, some cells as young as 5-7 generations acquired a greatly increased level of ROS detected by DHE relative to virgin cells. By 12 generations 50% of cells had a substantial ROS burden despite being only halfway through their lifespan. In contrast to the wild type, cells of a sir2 mutant had lower levels of ROS reacting with DHE. Daughter from older mothers had low ROS levels, and this asymmetric distribution of ROS was SIR2-independent. Mitochondrial fragmentation also began to occur in cells after 4 generations and increased markedly as cells aged. Daughter cells regenerated normal tubular mitochondria despite the fragmentation of mitochondria in the mother cells, while daughter of the sir2 mutant had fragmented mitochondria at all ages.CI - Copyright A(c) 2010. Published by Elsevier Inc.
|Status: Published||Type: Journal Article||PubMed ID: 21255640|
Topics addressed in this paper
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