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Briones C and Ballesta JP  (2000) Conformational changes induced in the Saccharomyces cerevisiae GTPase-associated rRNA by ribosomal stalk components and a translocation inhibitor. Nucleic Acids Res 28(22):4497-505

Abstract: The yeast ribosomal GTPase associated center is made of parts of the 26S rRNA domains II and VI, and a number of proteins including P0, P1alpha, P1beta, P2alpha, P2beta and L12. Mapping of the rRNA neighborhood of the proteins was performed by footprinting in ribosomes from yeast strains lacking different GTPase components. The absence of protein P0 dramatically increases the sensitivity of the defective ribosome to degradation hampering the RNA footprinting. In ribosomes lacking the P1/P2 complex, protection of a number of nucleotides is detected around positions 840, 880, 1100, 1220-1280 and 1350 in domain II as well as in several positions in the domain VI alpha-sarcin region. The protection pattern resembles the one reported for the interaction of elongation factors in bacterial systems. The results exclude a direct interaction of these proteins with the rRNA and are compatible with an increase in the ribosome affinity for EF-2 in the absence of the acidic P proteins. Interestingly, a sordarin derivative inhibitor of EF-2 causes an opposite effect, increasing the reactivity in positions protected by the absence of P1/P2. Similarly, a deficiency in protein L12 exposes nucleotides G1235, G1242, A1262, A1269, A1270 and A1272 to chemical modification, thus situating the protein binding site in the most conserved part of the 26S rRNA, equivalent to the bacterial protein L11 binding site.

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't PubMed ID: 11071938

Topics addressed in this paper

Number of different genes curated to this paper: 101

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Topics Genes linked to topics (#1 - 10 )
ARX1 ASC1 EFT1 EFT2 JJJ1 NIP7 NMD3 RDN18-1 RDN18-2 RDN25-1
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Topics Genes linked to topics (#11 - 20 )
RDN25-2 RDN5-1 RDN5-2 RDN5-3 RDN5-4 RDN5-5 RDN5-6 RDN58-1 RDN58-2 REH1
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DNA/RNA Sequence Features blue ball
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Non-Fungal Related Genes/Proteins blue ball
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Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#21 - 30 )
REI1 RPL10 RPL11A RPL11B RPL12A RPL12B RPL13A RPL13B RPL14A RPL14B
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Function/Process blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A RPL18B RPL19A RPL19B
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#41 - 50 )
RPP0 RPP1A RPP1B RPP2A RPP2B RPS0A RPS0B RPS10A RPS10B RPS11A
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Topics Genes linked to topics (#51 - 60 )
RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16A RPS16B RPS17A RPS17B
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Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#61 - 70 )
RPS18A RPS18B RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B
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Topics Genes linked to topics (#71 - 80 )
RPS22A RPS22B RPS23A RPS23B RPS24A RPS24B RPS25A RPS25B RPS26A RPS26B
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Topics Genes linked to topics (#81 - 90 )
RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30A RPS30B RPS31
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#91 - 100 )
RPS4A RPS4B RPS5 RPS6A RPS6B RPS7A RPS7B RPS8A RPS8B RPS9A
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#101 )
RPS9B
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