Schmidt S, et al. (2011) Identification of a Saccharomyces cerevisiae Glucosidase That Hydrolyzes Flavonoid Glucosides. Appl Environ Microbiol 77(5):1751-7
Abstract: Bakers yeast (Saccharomyces cerevisiae) whole-cell bioconversions of naringenin 7-O-beta- glucoside revealed considerable beta-glucosidase activity which impairs any strategy to generate or modify flavonoid glucosides in yeast transformants. Up to ten putative glycoside hydrolases annotated in the S. cerevisiae genome database were overexpressed with His-tags in yeast cells. Examination of these recombinant, partially purified polypeptides for hydrolytic activity with synthetic chromogenic alpha- or beta-glucosides identified three efficient beta-glucosidases (EXG1, SPR1 and YIR007W) which were further assayed with natural flavonoid beta-glucoside substrates and product verification by TLC or HPLC. Preferential hydrolysis of 7- or 4'-O-glucosides of isoflavones, flavonols, flavones and flavanones was observed in vitro with all three glucosidases, while anthocyanins were also accepted as substrates. The glucosidase activities of EXG1 or SPR1 were completely abolished by Val168Tyr mutation, which confirmed the relevance of this residue as reported for other glucosidases. Most importantly, biotransformation experiments with knock-out yeast strains revealed that solely EXG1 knock-out strains lost the capability of hydrolyzing flavonoid glucosides.
|Status: Published||Type: Journal Article||PubMed ID: 21216897|
Topics addressed in this paper
Number of different genes curated to this paper: 10
- To find other papers on a gene and topic, click on the colored ball in the appropriate box.
- displays other papers with information about that topic for that gene.
- displays other papers in SGD that are associated with that topic.
The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
- To go to the Locus page for a gene, click on the gene name.