SGD Paper 
Kurita T, et al. (2011) Kre6 Protein Essential for Yeast Cell Wall {beta}-1,6-Glucan Synthesis Accumulates at Sites of Polarized Growth. J Biol Chem 286(9):7429-38
Abstract: Saccharomyces cerevisiae Kre6 is a type II membrane protein with amino acid sequence homology with glycoside hydrolase and is essential for beta-1,6-glucan synthesis as revealed by the mutant phenotype, but its biochemical function is still unknown. The localization of Kre6 determined by epitope tagging is a matter of debate. We raised anti-Kre6 rabbit antiserum, and examined the localization of Kre6 and its tagged protein by immunofluorescence microscopy, subcellular fractionation in sucrose density gradients, and immunoelectron microscopy. Integration of the results indicate that the majority of Kre6 is in the endoplasmic reticulum, however, a small but significant portion is also present in the secretory vesicle-like compartments and plasma membrane. Kre6 in the latter compartments is observed as strong signals that accumulate at the sites of polarized growth by immunofluorescence. The truncated Kre6 without the N-terminal 230-amino acid cytoplasmic region did not show this polarized accumulation and had a severe defect in beta-1,6-glucan synthesis. This is the first evidence of a beta-1,6-glucan-related protein showing the polarized membrane localization that correlates with its biological function.
| Status: Published | Type: Journal Article | PubMed ID: 21193403 |
Topics addressed in this paper
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